Digital Commons Network^™

Evaluating Methods Of Preserving Aquatic Invertebrates For Microbiome Analysis, Stephanie N. Vaughn, Colin R. Jackson

Faculty and Student Publications

Research on the microbiomes of animals has increased substantially within the past decades. More recently, microbial analyses of aquatic invertebrates have become of increased interest. The storage method used while collecting aquatic invertebrates has not been standardized throughout the scientific community, and the effects of common storage methods on the microbial composition of the organism is unknown. Using crayfish and dragonfly nymphs collected from a natural pond and crayfish maintained in an aquarium, the effects of two common storage methods, preserving in 95% ethanol and freezing at −20◦C, on the invertebrate bacterial microbiome was evaluated. We found that the bacterial …

Writing Through A Place, Elsa Nekola

Yalobusha Review

A Conversation with Elsa Nekola

Pa-La-La-La, Juliet Gelfman-Randazzo

Yalobusha Review

No abstract provided.

Providing Culturally Competent Eldercare For Asian Americans, Britney Ngo

Honors Theses

As the Asian American elderly population continues to grow, it is important to acknowledge cultural factors that may impact their behavior, attitudes, preferences, and decisions in the context of eldercare. Cultural competency acknowledges and considers these factors while providing care that includes biophysical factors of healthcare as well as non-medical dimensions of care such as language and culture.

This thesis seeks to address the applications of providing culturally competent eldercare to Asian Americans and offer future recommendations to care settings that have limited resources by utilizing a multidisciplinary review of literature accompanied by interviews with representatives from On Lok, a …

Newsletter, March 2022, Cedar Oaks Guild

Cedar Oaks Guild Newsletter

No abstract provided.

Issue 390: March 24-April 7, 2022, The Local Voice

The Local Voice

No abstract provided.

Consensus Definition Of Misophonia: A Delphi Study, Susan E. Swedo, David M. Baguley, Damiaan Denys, Laura J. Dixon, Mercede Erfanian, Alessandra Fioretti, Pawel J. Jastreboff, Sukhbinder Kumar, M. Zachary Rosenthal, Romke Rouw, Daniela Schiller, Julia Simner, Eric A. Storch, Steven Taylor, Kathy R. Vander Werff, Cara M. Altimus, Sylvina M. Raver

Faculty and Student Publications

Misophonia is a disorder of decreased tolerance to specific sounds or their associated stimuli that has been characterized using different language and methodologies. The absence of a common understanding or foundational definition of misophonia hinders progress in research to understand the disorder and develop effective treatments for individuals suffering from misophonia. From June 2020 through January 2021, the authors conducted a study to determine whether a committee of experts with diverse expertise related to misophonia could develop a consensus definition of misophonia. An expert committee used a modified Delphi method to evaluate candidate definitional statements that were identified through a …

North Carolina Author Set For Annual Howry Lecture, Lydia Lagarde

University of Mississippi News

Sharon Holland is renowned for her writings on Blackness and sexuality

Machines, Intimacy Topics For Two Hybrid Oxford Science Cafes, Edwin B. Smith

University of Mississippi News

Computer science, neuroscience professors to present during in-person and virtual events

Issue 389: March 10-24, 2022, The Local Voice

The Local Voice

No abstract provided.

In Silico Tools For Analysis Of Single-Nucleotide Polymorphisms In The Bovine Transferrin Gene, Aarif Ali, Muneeb U. Rehman, Syed Mudasir Ahmad, Tabish Mehraj

Faculty and Student Publications

Dairy cattle with a high milk yield are susceptible to many infectious diseases, such as mastitis. Subclinical mastitis (SCM) is the most prevalent form of mastitis that predominantly affects animal health, and causes adverse effects on the quality and quantity of milk. In dairy animals, subclinical mastitis often remains undetected, as no gross changes in udder characteristics are visible. In the present study, 135 Holstein Friesian dairy animals were selected and screened as healthy (n = 25) and mastitic (n = 110) based on diagnostic tests such as the California mastitis test, pH, electrical conductivity, and somatic cell count. In …

Issue 388: February 24-March 10, 2022, The Local Voice

The Local Voice

No abstract provided.

Meet Sherrie Gussow, February's Staff Member Of The Month, Inside Ole Miss

University of Mississippi News

No abstract provided.

A Multitarget Approach To Evaluate The Efficacy Of Aquilaria Sinensis Flower Extract Against Metabolic Syndrome, Hee Sung Chae, Olivia Dale, Tahir Maqbool Mir, Bharathi Avula

Faculty and Student Publications

Aquilaria sinensis (Lour.) Spreng is known for its resinous secretion (agarwood), often secreted in defense against injuries. We investigated the effects of A. sinensis flower extract (AF) on peroxisome proliferator-activated receptors alpha and gamma (PPARα and PPARγ), liver X receptor (LXR), glucose uptake, and lipid accumulation (adipogenesis). Activation of PPARα, PPARγ and LXR was determined in hepatic (HepG2) cells by reporter gene assays. Glucose uptake was determined in differentiated muscle (C2C12) cells using 2-NBDG (2-deoxy-2-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]-D-glucose). Adipogenesis was determined in adipocytes (3T3-L1 cells) by Oil red O staining. At a concentration of 50 µg/mL, AF caused 12.2-fold activation of PPARα and …

Ranking The Biases: The Choice Of Otus Vs. Asvs In 16s Rrna Amplicon Data Analysis Has Stronger Effects On Diversity Measures Than Rarefaction And Otu Identity Threshold, Marlène Chiarello, Mark Mccauley, Sébastien Villéger, Colin R. Jackson

Faculty and Student Publications

Advances in the analysis of amplicon sequence datasets have introduced a methodological shift in how research teams investigate microbial biodiversity, away from sequence identity-based clustering (producing Operational Taxonomic Units, OTUs) to denoising methods (producing amplicon sequence variants, ASVs). While denoising methods have several inherent properties that make them desirable compared to clustering-based methods, questions remain as to the influence that these pipelines have on the ecological patterns being assessed, especially when compared to other methodological choices made when processing data (e.g. rarefaction) and computing diversity indices. We compared the respective influences of two widely used methods, namely DADA2 (a denoising …

Donoghue Collection Brings Legends Of Blues To Life, Rebecca Lauck Cleary

Blog Posts

Archives and Special Collections opens exhibition of memorabilia related to Sonny Boy Williamson. Sonny Boy Williamson soap and incense, test pressings of Memphis Slim and Buddy Holly records, and a signed contract by B.B. King are only a few of the many items recently donated by the family of William "Bill" Donoghue to the J.D. Williams Library at the University of Mississippi.

Meet Charlie Miles, January's Staff Member Of The Month, Inside Ole Miss

University of Mississippi News

No abstract provided.

Issue 386: January 27-February 10, 2022, The Local Voice

The Local Voice

No abstract provided.

Donoghue Collection Brings Legends Of Blues To Life, Rebecca Lauck Cleary

University of Mississippi News

No abstract provided.

Designing A Clinical Study With Dietary Supplements: It's All In The Details, Z. Elizabeth Floyd, David M. Ribnicky, Ilya Raskin, Daniel S. Hsia

Faculty and Student Publications

A successful randomized clinical trial of the effect of dietary supplements on a chosen endpoint begins with developing supporting data in preclinical studies while paying attention to easily overlooked details when planning the related clinical trial. In this perspective, we draw on our experience studying the effect of an ethanolic extract from Artemisia dracunculus L. (termed PMI-5011) on glucose homeostasis as a potential therapeutic option in providing resilience to metabolic syndrome (MetS). Decisions on experimental design related to issues ranging from choice of mouse model to dosing levels and route of administration in the preclinical studies will be discussed in …

Issue 385: January 10-27, 2022, The Local Voice

The Local Voice

Local Favorites Awards Results

Phytochemical Investigation Of Egyptian Riverhemp: A Potential Source Of Antileukemic Metabolites, Shimaa M. Abdelgawad, Mona H. Hetta, Mohamed A. Ibrahim, Premalatha Balachandran

Faculty and Student Publications

As part of our research group's continuous efforts to find alternative treatments for cancer, the aqueous ethanol extract of Sesbania sesban L. Merr. (SS, Egyptian riverhemp) demonstrated an antileukemic activity against K562 cell line. Bioguided fractionation of SS leaves hydroethanolic extract resulted in the isolation of one new compound (33) named as hederatriol 3-O-β-D-glucuronic acid methyl ester as well as 34 known compounds. Seven compounds ((34), (22), (20), (24), (21), (19), and (35)) showed high antiproliferative effects (IC50 = 22.3, 30.8, 31.3, 33.7, 36.6, 37.5, and 41.5 μM, respectively), while four compounds ((32), (5), (29), and (1)) showed milder activities …

Age-Related Neuroendocrine, Cognitive, And Behavioral Co-Morbidities Are Promoted By Hiv-1 Tat Expression In Male Mice, Alaa N. Qrareya, Fakhri Mahdi, Marc J. Kaufman, Nicole M. Ashpole

Faculty and Student Publications

In the U.S. about half of the HIV-infected individuals are aged 50 and older. In men living with HIV, secondary hypogonadism is common and occurs earlier than in seronegative men, and its prevalence increases with age. While the mechanisms(s) are unknown, the HIV-1 trans-activator of transcription (Tat) protein disrupts neuroendocrine function in mice partly by dysregulating mitochondria and neurosteroidogenesis. We hypothesized that conditional Tat expression in middle-aged male transgenic mice [Tat(+)] would promote age-related comorbidities compared to age-matched controls [Tat(−)]. We expected Tat to alter steroid hormone milieu consistent with behavioral deficits. Middle-aged Tat(+) mice had lower circulating testosterone and …

Identification Of An Orally Bioavailable, Brain-Penetrant Compound With Selectivity For The Cannabinoid Type 2 Receptor, Meirambek Ospanov, Suresh P. Sulochana, Jason J. Paris, John M. Rimoldi

Faculty and Student Publications

Modulation of the endocannabinoid system (ECS) is of great interest for its therapeutic relevance in several pathophysiological processes. The CB2 subtype is largely localized to immune effectors, including microglia within the central nervous system, where it promotes anti-inflammation. Recently, a rational drug design toward precise modulation of the CB2 active site revealed the novelty of Pyrrolo[2,1-c][1,4]benzodiazepines tricyclic chemotype with a high conformational similarity in comparison to the existing leads. These compounds are structurally unique, confirming their chemo-type novelty. In our continuing search for new chemotypes as selective CB2 regulatory molecules, following SAR approaches, a total of 17 selected (S,E)-11-[2-(arylmethylene)hydrazono]-PBD analogs …

Dual Drug Loaded Lipid Nanocarrier Formulations For Topical Ocular Applications, Ahmed Adel Ali Youssef, Narendar Dudhipala, Soumyajit Majumdar

Faculty and Student Publications

Introduction: Untreated ocular infections can damage the unique fine structures of the eye with possible visual impairments and blindness. Ciprofloxacin (CIP) ophthalmic solution is prescribed as first-line therapy in ocular bacterial infections. Natamycin (NT) ophthalmic suspension is one of the progenitors in ocular antifungal therapy. Nanostructured lipid carriers (NLCs) have been widely examined for ocular penetration enhancement and distribution to deeper ocular tissues. The objective of the current study was to prepare NLCs loaded with a combination of CIP and NT (CIP-NT-NLCs) and embed them in an in-situ gelling system (CIP-NT-NLCs-IG). This novel formulation will target the co-delivery of CIP …

Research News, Publications, 2022, Volume 4, Soumyajit Majumdar

Publications (2015-)

Publications that appeared during the period of October 1 through December 31, 2022

Uv Exposure Causes Energy Trade-Offs Leading To Increased Chytrid Fungus Susceptibility In Green Tree Frog Larvae, Rebecca L. Cramp, Michel E. B. Ohmer, Craig E. Franklin

Faculty and Student Publications

Levels of ultraviolet (UV) radiation have increased in many parts of the world due to the anthropogenic destruction of the ozone layer. UV radiation is a potent immunosuppressant and can increase the susceptibility of animal hosts to pathogens. UV radiation can directly alter immune function via immunosuppression and photoimmunotolerance; however, UV may also influence pathogen defences by affecting the distribution of energy resources among competing physiological processes. Both defence against UV damage and repair of incurred damage, as well as the maintenance of immune defences and responding to an immune challenge, are energetically expensive. These competing demands for finite energy …

Southern Register. 2022.1 (Winter 2022), University Of Mississippi. Center For The Study Of Southern Culture

Southern Register

No abstract provided.

Good Love Is Black: Stories On Black Queer Living In The American South, Danielle Buckingham

Electronic Theses and Dissertations

Black Queer and Trans folks conjure love in their lives, by any means necessary, even in the face of terror. This is what has been at the root of their survival against the anti-Blackness and heterosexism that permeates the nation. Good Love Is Black, highlights their journeys navigating multiple marginalized identities, particularly in the American South. For this project, I spoke with folks who are natives of or currently residing in Mississippi, Alabama, South Carolina, Texas, and Kentucky. This written thesis accompanies a narrative podcast that explores the ways in which their Black, Queer, and Southern Identity informs how they …

"How It Was Made", Bethany Fitts

Electronic Theses and Dissertations

This is a meta-autoethnography describing the researcher’s unfocused, rhizomatic research process throughout the COVID19 pandemic. Themes explored are the COVID19 pandemic, family, death, climate change, queer love, represenation, academia, genre, and religion. The study shows how multiple scales of grief arise during the research process. I locate this method within postcolonial theory of autoethnography that seeks to diversify structures within the academic genre.