Digital Commons Network^™

Digital (Re)Production: Spatial Mediations Through Augmented Reality Landscapes In ‘Pokemon Go’, Brigitte Pulminar Perkins

Theses and Dissertations--Geography

Since 2016, the mobile augmented reality game Pokémon GO has renewed interest within communities by mediating spatial engagement using in-game markers that must be engaged with throughout physical urban landscapes. This thesis identifies factors that influence the spatial distribution of digital markers used by this game to help inform analyses of urban gamification, urban spatial distribution, and cultural knowledge production processes. Drawing specifically on theories of urban spatialization and digital media studies, I highlight features of physical and digital legacies of redlining within Lexington, KY that are reproduced via Pokémon GO. I argue that the urban racialized landscape of Lexington …

State Governance And Less-Skilled Migrant Workers In South Korea, Weejun Park

Theses and Dissertations--Geography

This dissertation examines the migration governance of South Korea with a focus on the immigration of less-skilled migrant workers. While geographers and broader social scientists have explored this topic, previous studies have two main foci: 1) individual immigrants and their activities; and 2) migration policy development. Despite these studies’ contribution to the scholarship, there seems to be a relative lack of interest in the South Korean state as a potentially crucial actor in governing less-skilled migrant workers. To address this gap, this dissertation draws upon (political) geography, related social sciences, and interdisciplinary fields such as border studies, migration studies, and …

Polymorphism And Plasticity In The Termite Caste System, Austin Merchant

Theses and Dissertations--Entomology

Developmental plasticity, manifested as a caste system, is integral to the success of eusocial insects because of the way in which it decouples the various tasks that a single, solitary organism would normally be responsible for, allowing individuals to specialize towards their given roles while keeping vulnerable members of the colony, such as those responsible for reproduction, safe from harm. Termites exhibit particularly complex caste systems that can be divided into three types of castes: workers perform manual labor, such as foraging and brood care; soldiers defend the nest; and reproductives lay eggs. Each of these castes, as well as …

An Rnai-Based Approach To Ips Calligraphus Management: A Promising Strategy For Forest Protection, Mary Wallace

Theses and Dissertations--Entomology

As climate change leads to more frequent and severe extreme weather, host tree stress is increasing the occurrence of large-scale bark beetle outbreaks. One such beetle, Ips calligraphus (Germar), a native bark beetle in the southeastern United States, has traditionally been considered a secondary pest of pine trees. Management has relied on silvicultural practices that enhance forest resilience, enabling host trees to effectively use natural defenses to deter beetle infestation. However, during periods of forest stress caused by storms, droughts, human disturbance, and other factors, beetle outbreaks can cause considerable ecological and economic damage.

Addressing worsening I. calligraphus outbreaks will …

A Cotyledon-Based Virus-Induced Gene Silencing (Cotyledon-Vigs) Approach To Study Specialized Metabolism In Medicinal Plants, Yongliang Liu, Ruiqing Lyu, Joshua Singleton, Barunava Patra, Sitakanta Pattanaik, Ling Yuan

Plant and Soil Sciences Faculty Publications

Background Virus-induced gene silencing (VIGS) is widely used in plant functional genomics. However, the efficiency of VIGS in young plantlets varies across plant species. Additionally, VIGS is not optimized for many plant species, especially medicinal plants that produce valuable specialized metabolites.

Results We evaluated the efficacy of five-day-old, etiolated seedlings of Catharanthus roseus (periwinkle) for VIGS. The seedlings were vacuum-infiltrated with Agrobacterium tumefaciens GV3101 cells carrying the tobacco rattle virus (TRV) vectors. The protoporphyrin IX magnesium chelatase subunit H (ChlH) gene, a key gene in chlorophyll biosynthesis, was used as the target for VIGS, and we observed yellow cotyledons 6 …

Navigating The Crooked Road: A Case-Study Into The Appalachian Regional Commission’S Strategy To Promote Economic Development Through Cultural Tourism, Nicholas Relich

Gaines Fellow Senior Theses

Since 1965, the federal government’s Appalachian Regional Commission (ARC) has sought to improve Appalachia’s wellbeing by funding state and local economic development projects. One recent ARC strategy is investing in projects to develop the region’s tourism sector as a means of growth, particularly “cultural tourism,” which markets Appalachia’s cultural heritage to potential visitors. This paper seeks to evaluate the effectiveness of such ARC investments based on economic theory and by performing an empirical and qualitative case-study of one such series of projects: The Crooked Road, a music heritage trail located in Southwest Virginia. Officially organized in 2004 and receiving substantial …

Redefining Ed Utilization: A Rabies Post-Exposure Prophylaxis Perspective, David Bennington

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

Rabies, though rare in the United States, remains a lethal viral disease with significant public health implications. Post-exposure prophylaxis (PEP) through timely administration of rabies vaccines and rabies immune globulin (RIG) is critical for preventing the progression of the virus following potential exposure. However, the current system of healthcare delivery in the United States has witnessed an alarming trend: the unnecessary utilization of emergency department (ED) visits for rabies PEP, including follow-up vaccinations. This paper aims to examine and address this issue through a review of the factors contributing to the inappropriate use of ED services for rabies treatment.

Through …

Zebrafish Drug Screening Identifies Erlotinib As An Inhibitor Of Wnt/ Β-Catenin Signaling And Self-Renewal In T-Cell Acute Lymphoblastic Leukemia, Majd A. Al-Hamaly, Anna H. Cox, Meghan G. Haney, Wen Zhang, Emma C. Arvin, Shilpa Sampathi, Mary Wimsett, Chunming Liu, Jessica S. Blackburn

Markey Cancer Center Faculty Publications

The Wnt/β-catenin pathway’s significance in cancer initiation, progression, and stem cell biology underscores its therapeutic potential. However, the clinical application of Wnt inhibitors remains limited due to challenges posed by off-target effects and complex cross-talk of Wnt signaling with other pathways. In this study, we leveraged a zebrafish model to perform a robust and rapid drug screening of 773 FDA-approved compounds to identify Wnt/β-catenin inhibitors with minimal toxicity. Utilizing zebrafish expressing a Wnt reporter, we identified several drugs that suppressed Wnt signaling without compromising zebrafish development. The effi- cacy of the top hit, Erlotinib, extended to human cells, where it …

A Case Study In Prospective Program Evaluation, Sarai Rosemary Combs

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

Evaluation is a helpful tool in public health to provide transparency about community interventions and programs. The following is a prospective evaluation of a case study, looking at a recently designed program by the Lexington-Fayette County Health Department, as a capstone project for the Health Management and Policy concentration of the Masters in Public Health program of the College of Public Health. The health department developed a series of online training modules to increase knowledge of Methicillin-resistant staphylococcus aureus (MRSA) and other similar organism outbreaks and how to respond to these outbreaks. The program targets were Fayette County Public School …

Characteristics Of Congenital Syphilis Cases In Kentucky 2019-2023, Ines Roy

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

Abstract:

Background: The incidence of congenital syphilis (CS) has increased both in the United States (US) and globally and remains a public health concern. In Kentucky alone, there has been a significant increase in CS cases, from 9 cases in 2019 to 27 cases in 2023. CS is a preventable disease, but testing and immediate treatment are critical to prevent its spread. We have limited knowledge regarding Kentucky’s characteristics and behavioral factors that may be associated with this rise. The goal of this study is to identify characteristics and potential risk factors that contribute to the rise of CS in …

The Distribution Of Cp-Cre Cases From 2013-2020 In The Commonwealth Of Kentucky, Hannah Hiscox

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

Multidrug-resistant organisms (MDROs), particularly Carbapenem-Resistant Enterobacterales (CRE), pose a significant threat to public health due to their resistance to multiple antibiotics, including carbapenems. CRE infections, especially those producing carbapenemases (CP-CRE), are associated with high morbidity and mortality rates, making them a growing concern in healthcare settings. Despite the prevalence of CRE infections in the United States, limited data exist on the incidence and distribution of CP-CRE infections in specific regions, such as the Appalachian counties of Kentucky.

This study aimed to investigate the cumulative incidence and distribution of CP-CRE cases in Appalachian and non-Appalachian counties of Kentucky, as well as …

A Case Study In Program Evaluation, Iantha Wright

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

The framework for program evaluation is a systematic way of improving and accounting for public health actions by providing practical, feasible, ethical, and accurate information. As a practical, non-prescriptive tool, it guides public health professionals through the essential steps in program evaluation. The framework uses the six steps to understand how to improve and conduct assessments effectively. Additionally, it emphasizes integrating stakeholders and using ongoing evaluation strategies to plan effective interventions, improve existing programs, and demonstrate the efficient use of resources.

Demographic And Behavioral Characteristics Of People Who Help Others Inject Drugs: A Study Of People Who Inject Drugs In Appalachia Kentucky, Ryli Hockensmith

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

Aim: This study aims to understand the demographic and behavioral characteristics of people who inject drugs (PWID) who assist others with injection, hereafter “injection assistors,” and assess the characteristics of injection assistors compared to PWID who do not assist others with injection in Appalachian Kentucky.

Methods: A cross sectional secondary data analysis of data collected from 433 PWID enrolled in the ongoing Kentucky Outreach Service (KyOSK) was performed. Logistic regression analyses were performed to compare injection assistors to those who do not assist others on demographic, behavioral, service usage, and harm reduction characteristics.

Results: In total, 128 (29%) PWID reported …

Mail-Based Or Home Delivery Services: Preferences Among People Who Inject Drugs In Appalachian Kentucky, Su Su Zin

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

Aim: To explore acceptability of delivery-based syringe services program (SSP) among people who inject drug (PWID) in Eastern Kentucky and compare those who wanted to participate in delivery-based SSP to those who did not.

Methods: This was a cross-sectional analysis of data from PWID who injected drugs to get high in the past 30 days (n=246) collected during the baseline assessment administered to the Gateway2Health cohort. Chi-square tests, t-tests, univariate and multivariable logistic regressions were calculated between independent variables and the outcome variable (likelihood of participating in a delivery-based SSP).

Results: 52.9% of PWID reported being likely to participate in …

Human Papilloma Virus Type 16 Seroprevalence Among Men Living With Hiv, Ashley Duff

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

Objectives: Individuals living with HIV are at a higher risk for developing human papillomavirus-driven oropharyngeal cancer (HPV-OPC). There are no methods for early detection; however, HPV16 E6 antibodies have been identified as a promising early marker. The objective of this study was to evaluate seroprevalence and predictors of HPV16 E6 seropositivity among men living with HIV.

Methods:2,334 men aged 40+ living with HIV with blood specimens banked within the Tennessee Center for AIDS Research (TN-CFAR) biorepository were evaluated using HPV multiplex serology. Seroprevalence of HPV16 antibodies was calculated for all HPV16 proteins analyzed (L1, E1, E2, E4, E6, E7). …

A Case Study In Program Evaluation, Sierra L. Williams

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

The Lexington-Fayette County Health Department (LFCHD) developed an education program targeted toward infectious disease outbreaks. The program ensures timely reporting and action during an infectious disease outbreak within the Fayette County Public Schools (FCPS) system. I have been tasked with evaluating the program and its implementation of training modules through pre and post-surveys to determine if the program is significantly increasing the overall knowledge of program participants and make recommendations for how program aspects can be improved.

Program Evaluation Case Study, Grace Mullikin

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

Program evaluation case study utilizing Center for Disease Control and Prevention's Case Evaluation Framework.

The Health Literacy Practices Of Rehabilitation Providers Of Perioperative Orthopedic Education, Allen Keener

Theses and Dissertations--Rehabilitation Sciences

Health literacy is the ability to utilize information to make decisions about one’s health or the health of others. It is estimated that a large percentage of the population has inadequate health literacy, yet healthcare professionals, including rehabilitation providers often do not have procedures in place for assessing and addressing the health literacy of their patients. Related to orthopedic patients, a large portion of the interventions are educational in nature. Therefore, it is important that the health literacy of orthopedic patients be considered when addressing their needs perioperatively. There is little currently published related to health literacy among rehabilitation providers …

An Uncultivated Skillset: Emotional Intelligence In Healthcare Profession Students, Molly Taylor

Theses and Dissertations--Rehabilitation Sciences

Burnout and social isolation have become critical threats to optimal health and well-being, with detrimental effects specifically impacting healthcare profession (HCP) students and providers. The US Surgeon General has labelled burnout a crisis in the health workforce and named loneliness an epidemic with profound consequences for mortality and collective longevity. HCP students are heavily and disproportionately affected by stress and burnout compared to their peers, resulting in negative consequences on academic performance, mental health, empathy, and compassion satisfaction. Emotional Intelligence (EI) is a learnable skillset shown to protect against burnout and enhance relational skills, well-being, personal and professional satisfaction, and …

Equestrian Activities And Health Outcomes: A Multidimensional Analysis Of Physical Activity, Injury Patterns, And Occupational Concerns, Michaela Keener

Theses and Dissertations--Rehabilitation Sciences

Over 6 million participate in equestrian activities each year. However, research focusing on the benefits and risks around equestrian activities is limited to epidemiological research and small sample sizes. Equestrian participation ranges from recreational to occupational, and little research has evaluated how the risks of participation change based on the level of participation. Special consideration needs to be given to the occupational equestrians as equestrian participation is their livelihood. The first two aims of this study were to evaluate the physical activity engagement, perceptions of equestrian as a sport, acute injury history, and chronic pain frequency of equestrians across all …

The Effect Of Adding Perceptual-Cognitive Load To Assessments And Intervention Strategies In Healthy Adults, Ke'la H. Porter

Theses and Dissertations--Rehabilitation Sciences

In collegiate athletes, approximately one in four injuries require more than seven days before the athlete can return to full participation. Additionally, there are significant long-term consequences associated with these injuries, such as pain and osteoarthritis. Despite the role that the sensorimotor system and cognitive function play in athletic competition, the interaction between these systems in relation to sport-related injury is commonly overlooked. The sensorimotor system integrates and processes sensory, motor, and central mechanisms to produce a motor response. Further, cognition constructs, such as visual attention or processing speed, allow athletes to navigate the chaotic sporting environment. When there are …

Physical And Psychosocial Factors Related To Firefighter Musculoskeletal Disorders, Jennifer Ellen Tinsley Kubala

Theses and Dissertations--Rehabilitation Sciences

Career firefighters must perform physically and psychologically challenging duties in unpredictable and dangerous conditions. Previous investigations have found that firefighters suffer nearly 4 times the recorded musculoskeletal disorders (MSD) and workplace injuries compared to private and public sector workers, and take approximately 1.5 days longer to return to normal work. Thus, the burden of firefighter MSD experiences has been a growing area of exploration. The few investigations that have concurrently examined physical and psychosocial consequences experienced by firefighters have found a positive relationship between increased occupational stress reports and MSD experiences. However, these investigations, as well as those that individually …

The Role Of Social Determinants Of Health On Patient Recovery After Critical Illness, Lindsey E. Fresenko

Theses and Dissertations--Rehabilitation Sciences

Patients who survive critical illness have a prolonged recovery which impacts their quality of life and outcomes. The sudden life changes from being critically ill may create or exacerbate social factors or needs also known as social determinants of health. Patients’ SDOH are defined as the environments in which we live, work and play that impact health outcomes. Patients’ SDOH impact their life in early recovery from changes in previous roles to their need for increased financial, physical and emotional support. During this early recovery, patients are often recommended to participate in rehabilitation to improve their physical function. Although, accessing …

Key To Success: A Comprehensive Examination Of Support Personnel In School-Based Speech-Language Telepractice, Hannah Douglass

Theses and Dissertations--Rehabilitation Sciences

In the school setting, the telepractice team primarily consists of the clinician and a support person known as a telefacilitator. Though telepractice is an accepted method for delivering school-based speech-language therapy, many barriers to the integration of telepractice as routine care persist. A major barrier is clinicians' acceptance of telehealth. However, there has been limited investigation of factors that influence clinician acceptance. Another barrier is related to support personnel. Telefacilitators have been shown to be an integral part of the telepractice program. Yet there are significant gaps in knowledge regarding the roles and responsibilities, staffing and management, and barriers/facilitators to …

A Mixed Methods Exploration Of Occupational Therapy Pratice And Multiple Sclerosis Specialization, Dana Alghadban

Theses and Dissertations--Rehabilitation Sciences

The aim of this dissertation is to increase the body of research of how occupational therapists can address multiple sclerosis rehabilitation through occupational therapy services. Multiple sclerosis is a neurodegenerative disease that creates challenges for individuals to engage in meaningful occupations and significantly affects many areas of their lives. Occupational therapists play a role in the rehabilitation process for people with multiple sclerosis by providing occupation-focused interventions that may improve the health and well-being of clients living with the disease. As a result of the unpredictability of symptoms experienced by this population, individualized approaches to addressing participation and occupational performance …

Agn Storm 2. Ix. Studying The Dynamics Of The Ionized Obscurer In Mrk 817 With High-Resolution X-Ray Spectroscopy, Fatima Zaidouni, Erin Kara, Peter Kosec, Missagh Mehdipour, Daniele Rogantini, Gerard A. Kriss, Ehud Behar, Jelle Kaastra, Aaron J. Barth, Edward M. Cackett, Gisella De Rosa, Yasaman Homayouni, Keith Horne, Hermine Landt, Nahum Arav, Misty C. Bentz, Michael S. Brotherton, Elena Dalla Bontà, Maryam Dehghanian, Gary Ferland, Carina Fian, Jonathan Gelbord, Michael R. Goad, Diego H. González Buitrago, Catherine J. Grier, Patrick B. Hall, Chen Hu, Dragana Ilić, Shai Kaspi, Christopher S. Kochanek, Andjelka B. Kovačević, Daniel Kynoch, Collin Lewin, John Montano, Hagai Netzer, Jack M. M. Neustadt, Christos Panagiotou, Ethan R. Partington, Rachel Plesha, Luka Č. Popović, Daniel Proga, Thaisa Storchi-Bergmann, David Sanmartim, Matthew R. Siebert, Matilde Signorini, Marianne Vestergaard, Tim Waters, Ying Zu

Physics and Astronomy Faculty Publications

We present the results of the XMM-Newton and NuSTAR observations taken as part of the ongoing, intensive multiwavelength monitoring program of the Seyfert 1 galaxy Mrk 817 by the AGN Space Telescope and Optical Reverberation Mapping 2 (AGN STORM 2) Project. The campaign revealed an unexpected and transient obscuring outflow, never before seen in this source. Of our four XMM-Newton/NuSTAR epochs, one fortuitously taken during a bright X-ray state has strong narrow absorption lines in the high-resolution grating spectra. From these absorption features, we determine that the obscurer is in fact a multiphase ionized wind with an outflow velocity of …

Agn Storm 2. Viii. Investigating The Narrow Absorption Lines In Mrk 817 Using Hst-Cos Observations, Maryam Dehghanian, Nahum Arav, Gerard A. Kriss, Missagh Mehdipour, Doyee Byun, Gwen Walker, Mayank Sharma, Aaron J. Barth, Misty C. Bentz, Benjamin D. Boizelle, Michael S. Brotherton, Edward M. Cackett, Elena Dalla Bontà, Gisella De Rosa, Gary Ferland, Carina Fian, Alexei V. Filippenko, Jonathan Gelbord, Michael R. Goad, Keith Horne, Yasaman Homayouni, Dragana Ilić, Michael D. Joner, Erin A. Kara, Shai Kaspi, Christopher S. Kochanek, Kirk T. Korista, Peter Kosec, Andjelka B. Kovačević, Hermine Landt, Collin Lewin, Ethan R. Partington, Luka Č. Popović, Daniel Proga, Daniele Rogantini, Matthew R. Siebert, Thaisa Storchi-Bergmann, Marianne Vestergaard, Timothy Waters, Jian-Min Wang, Fatima Zaidouni, Ying Zu

Physics and Astronomy Faculty Publications

We observed the Seyfert 1 galaxy Mrk 817 during an intensive multiwavelength reverberation mapping campaign for 16 months. Here, we examine the behavior of narrow UV absorption lines seen in the Hubble Space Telescope/ Cosmic Origins Spectrograph spectra, both during the campaign and in other epochs extending over 14 yr. We conclude that, while the narrow absorption outflow system (at −3750 km s−1 with FWHM = 177 km s−1) responds to the variations of the UV continuum as modified by the X-ray obscurer, its total column density (log NH = 19.5 - +0.13 0.61 cm−2) did not change across all …

Agn Storm 2. Vi. Mapping Temperature Fluctuations In The Accretion Disk Of Mrk 817, Jack M. M. Neustadt, Christopher S. Kochanek, John Montano, Jonathan M. Gelbord, Aaron J. Barth, Gisella De Rosa, Gerard A. Kriss, Edward M. Cackett, Keith Horne, Erin A. Kara, Hermine Landt, Hagai Netzer, Nahum Arav, Misty C. Bentz, Elena Dalla Bontà, Maryam Dehghanian, Pu Du, Rick Edelson, Gary Ferland, Carina Fian, Travis Fischer, Michael R. Goad, Diego H. González Buitrago, Varoujan Gorjian, Catherine J. Grier, Patrick B. Hall, Yasaman Homayouni, Chen Hu, Dragana Ilić, Michael D. Joner, Jelle Kaastra, Shai Kaspi, Kirk T. Korista, Andjelka B. Kovačević, Collin Lewin, Yan-Rong Li, Ian M. Mchardy, Missagh Mehdipour, Jake A. Miller, Christos Panagiotou, Ethan Partington, Rachel Plesha, Richard W. Pogge, Luka Č. Popović, Daniel Proga, Thaisa Storchi-Bergmann, David Sanmartim, Matthew R. Siebert, Matilde Signorini

Physics and Astronomy Faculty Publications

We fit the UV/optical lightcurves of the Seyfert 1 galaxy Mrk 817 to produce maps of the accretion disk temperature fluctuations δT resolved in time and radius. The δT maps are dominated by coherent radial structures that move slowly (v = c) inward and outward, which conflicts with the idea that disk variability is driven only by reverberation. Instead, these slow-moving temperature fluctuations are likely due to variability intrinsic to the disk. We test how modifying the input lightcurves by smoothing and subtracting them changes the resulting δT maps and find that most of the temperature fluctuations exist over relatively …

Correlations Of Event Activity With Hard And Soft Processes In P + Au Collisions At √S Nn = 200 Gev At The Rhic Star Experiment, Star Collaboration

Physics and Astronomy Faculty Publications

With the STAR experiment at the BNL Relativistic Heavy Ion Collider, we characterize √s NN = 200 GeV p + Au collisions by event activity (EA) measured within the pseudorapidity range η ∈ [−5, −3.4] in the Au-going direction and report correlations between this EA and hard- and soft-scale particle production at midrapidity (η ∈ [−1, 1]). At the soft scale, charged particle production in low-EA p + Au collisions is comparable to that in p + p collisions and increases monotonically with increasing EA. At the hard scale, we report measurements of high transverse momentum (pT ) jets in …

Collision-Energy Dependence Of Deuteron Cumulanorrelations In Au+Au Collisions At Rhicts And Proton-Deuteron C, The Star Collaboration

Physics and Astronomy Faculty Publications

We report the first measurements of cumulants, up to 4𝑡ℎ order, of deuteron number distributions and proton-deuteron correlations in Au+Au collisions recorded by the STAR experiment in phase-I of Beam Energy Scan (BES) program at the Relativistic Heavy Ion Collider. Deuteron cumulants, their ratios, and proton-deuteron mixed cumulants are presented for different collision centralities covering a range of center-of-mass energy per nucleon pair √𝑠NN = 7.7 to 200 GeV. It is found that the cumulant ratios at lower collision energies favor a canonical ensemble over a grand canonical ensemble in thermal models. An anti-correlation between proton and deuteron multiplicity is …