Digital Commons Network^™

Measuring The Contribution Of Bald Cypress “Knees” To Greenhouse Gas Emissions During Climate Extremes, Skylar Ross, Jessica B. Moon, Niklas Klauss, Marissa Miles, Kabi Raj Khatiwada4, Bassil El Masri, Gary Stinchcomb, Benjamin R. K. Runkle

Kentucky Water Resources Annual Symposium

No abstract provided.

Impacts Of Land Use And Groundwater Inputs On Biological Stream Health And Habitat Of Jennings Creek, Bowling Green, Kentucky, Allison Francis, Jason S. Polk

Kentucky Water Resources Annual Symposium

No abstract provided.

The Role Of Beavers In Mediating Short-Term Organic Matter Accretion In Kentucky Acep Wre Easements, Jessica B. Moon, Marissa Miles, Logan Firkins, Karen A. Baumann, Michael B. Flynn

Kentucky Water Resources Annual Symposium

No abstract provided.

Characterizing Louisville Wetlands From Above And Below To Better Predict Wetland Carbon Sink-Source Status, Andrea E. Gaughan, Andrew S. Mehring, Mark Tierney, David Brown, Kassidy Haynes, Madison Cicha

Kentucky Water Resources Annual Symposium

No abstract provided.

Temperature-Responsive Membrane With Nano-Sized Catalysts For The Treatment Of Contaminated Groundwater, Rollie Mills, Dibakar Bhattacharyya

Kentucky Water Resources Annual Symposium

No abstract provided.

Full Program Proceedings For 2023 Kentucky Water Resources Symposium, Kentucky Water Research Institute

Kentucky Water Resources Annual Symposium

No abstract provided.

Membrane Functionalization Approaches Toward Per- And Polyfluoroalkyl Substances And Selected Metal Ion Separations, Francisco Cecil Léniz-Pizarro, Holly E. Rudel, Nicolas J. Briot, Julie B. Zimmerman, Dibakar Bhattacharyya

UK CARES Faculty Publications

Adsorption and ion exchange technologies are two of the most widely used approaches to separate pollutants from water; however, their intrinsic diffusion limitations continue to be a challenge. Pore functionalized membranes are a promising technology that can help overcome these challenges, but the extents of their competitive benefits and broad applicability have not been systematically evaluated. Herein, three types of adsorptive/ion exchange (IX) polymers containing strong/weak acid, strong base, and iron-chitosan complex groups were synthesized in the pores and partially on the surface of microfiltration (MF) membranes and tested for the removal of organic and inorganic cations and anions from …

Evaluation Of Durable Pavement Striping, William Staats, Erin Lammers-Staats

Kentucky Transportation Center Research Report

Roadway pavement markings, colloquially referred to as “striping”, can be made of various combinations of paint and reflective beads. In the interest of aligning Kentucky with the state-of-the-art durable pavement marking practices of other states, the Kentucky Transportation Cabinet (KYTC) developed a research study aimed at improving pavement marking specifications and practices. This study reviewed current practices, identified potential improvements, and performed a systematic evaluation in a controlled environment on both asphalt and concrete roads. The state evaluated high-build waterborne paint, extruded and spray thermoplastic, polyurea, and preformed plastic tape, as well as several bead packages including M247, Missouri blend, …

Non-Contact Optical Spectroscopy For Tumor-Sensitive Diffuse Reflectance And Fluorescence Measurements On Murine Subcutaneous Tissue Models: Monte Carlo Modeling And Experimental Validations, Zahid Hasan, Pranto Soumik Saha, Madison O. Korfhage, Caigang Zhu

Markey Cancer Center Faculty Publications

Fiber-optic probes are commonly used in biomedical optical spectroscopy platforms for light delivery and collection. At the same time, it was reported that the inconsistent probe- sample contact could induce significant distortions in measured optical signals, which consequently cause large analysis errors. To address this challenge, non-contact optical spectroscopy has been explored for tissue characterizations. However, existing non-contact optical spectroscopy platforms primarily focused on diffuse reflectance measurements and may still use a fiber probe in which the probe was imaged onto the tissue surface using a lens, which serves as a non-contact probe for the measurements. Here, we report a …

A Lamprey Neural Cell Type Atlas Illuminates The Origins Of The Vertebrate Brain, Francesco Lamanna, Francisca Hervas-Sotomayor, A. Phillip Oel, David Jandzik, Daniel Sobrido-Cameán, Gabriel N. Santos-Durán, Megan L. Martik, Jan Stundl, Stephen A. Green, Thoomke Brüning, Katharina Mößinger, Julia Schmidt, Celine Schneider, Mari Sepp, Florent Murat, Jeramiah J. Smith, Marianne E. Bronner, María Celina Rodicio, Antón Barreiro-Iglesias, Daniel M. Medeiros, Detlev Arendt, Henrik Kaessmann

Markey Cancer Center Faculty Publications

The vertebrate brain emerged more than ~500 million years ago in common evolutionary ancestors. To systematically trace its cellular and molecular origins, we established a spatially resolved cell type atlas of the entire brain of the sea lamprey—a jawless species whose phylogenetic position affords the reconstruction of ancestral vertebrate traits—based on extensive single-cell RNA-seq and in situ sequencing data. Comparisons of this atlas to neural data from the mouse and other jawed vertebrates unveiled various shared features that enabled the reconstruction of cell types, tissue structures and gene expression programs of the ancestral vertebrate brain. However, our analyses also revealed …

Syne1 Mutation Is Associated With Increased Tumor Mutation Burden And Immune Cell Infiltration In Ovarian Cancer, Laura M. Harbin, Nan Lin, Frederick R. Ueland, Jill M. Kolesar

Markey Cancer Center Faculty Publications

SYNE1, a nuclear envelope protein critical for cellular structure and signaling, is downregulated in numerous malignancies. SYNE1 alterations are found in 10% of gynecologic malignancies and 5% of epithelial ovarian cancers. Previous studies demonstrated an association between SYNE1 mutation, increased tumor mutation burden (TMB), and immunotherapy response. This study evaluates the SYNE1 mutation frequency, association with TMB, and downstream effects of SYNE1 mutation in ovarian cancer. Genetic information, including whole-exome sequencing, RNA analysis, and somatic tumor testing, was obtained for consenting ovarian cancer patients at an academic medical center. Mutation frequencies were compared between the institutional cohort and The Cancer …

Interferon-Stimulated Gene Tdrd7 Interacts With Ampk And Inhibits Its Activation To Suppress Viral Replication And Pathogenesis, Sukanya Chakravarty, Gayatri Subramanian, Sonam Popli, Manoj Veleeparambil, Shumin Fan, Ritu Chakravarti, Saurabh Chattopadhyay

Markey Cancer Center Faculty Publications

Many viruses activate cellular autophagy in infected cells to facilitate their replication. Recently, we identified an interferon (IFN)-stimulated gene (ISG) Tudor domain containing 7 (TDRD7), which inhibits viral replication by blocking autophagy pathway. Here, we present a molecular mechanism for TDRD7 action and its relative contribution to protection against viral pathogenesis. TDRD7 inhibited the activation of adenosine monophosphate (AMP)-activated protein kinase (AMPK), a kinase required for initiating autophagy. Mechanistically, TDRD7 interacted directly with AMPK in the cytosolic compartment. Domain-mapping analyses revealed C-terminal Tudor domain of TDRD7 interacted with auto-inhibitory domain of AMPK. Deletion of Tudor domains abolished anti-AMPK and antiviral …

Unleashing The Potential Of 1,3-Diketone Analogues As Selective Lh2 Inhibitors, Juhoon Lee, Hou-Fu Guo, Shike Wang, Yazdan Maghsoud, Erik Antonio Vázquez-Montelongo, Zhifeng Jing, Rae M. Sammons, Eun Jeong Cho, Pengyu Ren, G. Andrés Cisneros, Jonathan M. Kurie, Kevin N. Dalby

Markey Cancer Center Faculty Publications

Lysyl hydroxylase 2 (LH2) catalyzes the formation of highly stable hydroxylysine aldehyde-derived collagen cross-links (HLCCs), thus promoting lung cancer metastasis through its capacity to modulate specific types of collagen cross-links within the tumor stroma. Using 1 and 2 from our previous high-throughput screening (HTS) as lead probes, we prepared a series of 1,3-diketone analogues, 1−18, and identified 12 and 13 that inhibit LH2 with IC50’s of approximately 300 and 500 nM, respectively. Compounds 12 and 13 demonstrate selectivity for LH2 over LH1 and LH3. Quantum mechanics/molecular mechanics (QM/MM) modeling indicates that the selectivity of 12 and 13 may stem from …

Kentucky Traffic Collision Facts 2022 Report, Paul Ross, Eric Green, Christopher Blackden, Kenneth R. Agent, Christopher Van Dyke

Kentucky Transportation Center Research Report

Kentucky’s Traffic Collision Facts is based on collision reports submitted to the Kentucky State Police Records Branch. As required by Kentucky Revised Statute 189.635: “Every law enforcement agency whose officers investigate a vehicle accident of which a report must be made...shall file a report of the accident...within ten days after investigation of the accident upon forms supplied by the bureau.” The stated purpose of this requirement is to utilize data on traffic collisions to improve the Commonwealth’s traffic safety program. Unless otherwise noted, data in this publication are for public roads only. Data contained in this report are based solely …

Inspection Training Course On Bridge Preventive Maintenance Activities, Danny Wells, Sudhir Palle

Kentucky Transportation Center Research Report

Despite over 30 percent of bridges in the United States having exceeded their 50-year design lives, most state departments of transportation (DOTs) lack the funding needed to replace bridges on a large scale. In response, agencies have increasingly turned to bridge preventive maintenance activities to prolong bridge service lives. These activities provide a safe and cost-effective way to slow the rate at which structures deteriorate, mitigate the effects of aging, and improve bridge functional condition. The Kentucky Transportation Cabinet (KYTC) has become increasingly reliant on preventive maintenance to preserve and extend the service lives of its steel and concrete bridges. …

2023 Safety Belt Usage Survey In Kentucky, Erin Lammers-Staats, Derek S. Young, Kenneth R. Agent, Aidan Elias

Kentucky Transportation Center Research Report

Data and results of a statewide observational survey used to establish the statewide usage rate for safety belts in Kentucky.

Analysis Of Traffic Crash Data In Kentucky 2018-2022, Paul Ross, Eric Green, Christopher Blackden, Christopher Van Dyke

Kentucky Transportation Center Research Report

This report documents analysis of traffic crash data in Kentucky. A primary objective of this study was to determine average crash statistics for Kentucky highways. Where used, rates were calculated for various highway types and for counties and cities. Difference criteria were used for exposure. Average and critical numbers, SPFs, and crash rates were calculated for various highway types in rural and urban areas. These metrics rely on crashes identified on highways where Annual Average Daily Traffic (AADT) volumes were available. Data in this report may be used to help identify problem areas. The other primary objective of this study …

Scale Model Experiments Of Toxic Gas Production From The Combustion Of Polymers When Applied With Different Droplet Sizes Of Water Mist, Nicharee Thinnakornsutubutr, Masayuki Mizuno, Kazunori Kuwana

Progress in Scale Modeling, an International Journal

This research experimentally investigated the combustion of polymeric materials with water mist application in an enclosure, with an emphasis on the production of toxic gases. Two different diameters, ~100 and ~260 μm, were tested. The experimental conditions were determined based on Froude similarity laws for low drop Reynolds number conditions. Droplets and polymers’ physical and chemical properties influence the burning/extinguishing behavior and toxic-gas evolution. In general, larger droplets can extinguish a fire in a shorter time, and toxic gas concentrations in a test chamber decreased more rapidly. However, the large droplets tended to cause the flame expansion phenomenon for thermoplastics …

Study On Transpiration Rates Of Vicia Villocea And Bromus Inermis Species, S. H. R. Sadeghi, N. Rahimzadeh

IGC Proceedings (1977-2023)

Ecohydrology is concerned with the interaction between the hydrological and plant processes. Some aspects of the hydrologic cycle, such as transpiration and interception have received little attention owing to difficulties in field measurements. Quantifying the components of water balance for a watershed is crucial for understanding the dominant hydrologic processes occurring in a basin (Flerchinger & Cooley, 2000). Water use by vegetation is controlled by the water uptake by roots, the transfer of liquid water through plants and vapour loss from the leaf surfaces by the opening and closure of the stomata (Roberts, 2000) i.e. transpiration. Comparison of transpiration of …

Mechanical Aeration And Liquid Dairy Manure: Application Impacts On Grassland Runoff Water Quality And Yield, T. J. Basden, S. B. Shah, J. L. Miller

IGC Proceedings (1977-2023)

Wet weather on heavy soils reduces oxygen availability in the root zone and reduces forage yields. Mechanical aeration can improve forage yield in these soil conditions. Research has shown that under certain conditions, mechanical aeration can increase yield by improving drainage and aeration (Davies et al., 1989); aeration can also increase depression, storage and infiltration thus reducing surface runoff and improving nutrient distribution in the root zone. Aeration on sloping, fertilised grassland can provide environmental (Douglas et al., 1995) and agronomic benefits. The objectives of this study were to evaluate the runoff water quality and agronomic impacts of …

Development Of A Toolkit For Participatory Management Of Rural Watersheds In Kenya, L. W. Chiuri, F. K. Lelo, M. W. Jenkins, S. N. Miller

IGC Proceedings (1977-2023)

Effective public participation is a foundation for sustainable watershed management, yet there are no demonstrated methods for or examples of its achievement in tropical semi-arid rural grassland watersheds of Kenya which support critical downstream water services. Within the Sustainable Management of Watersheds (SUMAWA) multidisciplinary international research project, a set of tools has been developed and tested to engage local communities and stakeholders in a dialogue and decision-making process to improve the development and management of the River Njoro Watershed in Kenya and reverse declining water quality and quantity problems. A toolkit manual based on the experience is under preparation for …

Participatory Plant Breeding In Uruguay, D. Real, G. A. Ferreira, D. F. Risso, C. Mas

IGC Proceedings (1977-2023)

The introduction of forage legumes into the native pastures of Uruguay is considered an environmentally safe method with which to increase the level of production through the addition of biologically fixed nitrogen. An integrated plant breeding programme was initiated at INIA (National Agricultural Research Institute), Uruguay, with the aim of developing forage legumes able to persist and produce in co-existence with the native (grass dominant) vegetation under cattle and sheep grazing. The programme was conducted in parallel with rhizobial strain selection. During 1998 to 2001 at Glencoe Research Station (32^o 01'32"S lat; 57^o 00^o 39"W long), 326 …

Adapting The Cropgro Model To Predict Growth And Perennial Nature Of Bahiagrass, S. J. Rymph, K. J. Boote, J. W. Jones

IGC Proceedings (1977-2023)

The objective of this research was to modify an existing crop growth model for ability to predict growth and composition of bahiagrass (Paspalm notatum Flügge) in response to daily weather and management inputs. The CROPGRO-CSM cropping systems model has a generic, process-oriented structure that allows inclusion of new species and simulating cropping sequences and crop rotations. An early adaptation of CROPGRO-CSM "species files" for bahiagrass over-predicted growth during late fall through early spring, and totally failed in re-growth if all foliage was lost from freeze damage. Revised species parameters and use of "pest damage" offered only a partial solution. …

An Interactive, Web-Based Module To Teach The Principles Of Silage Fermentation, M. H. Hall

IGC Proceedings (1977-2023)

The forage-livestock industry represents a major agricultural enterprise throughout the world. In the United States, forages comprise 40-60% of the dairy cow's diet with many producers feeding 50-100% of this forage as silage (Kempisty, 1997). Harvesting forages as silage continues to increase as the size of farms increase and the mechanisation of silage harvesting allows timely harvest of a greater area. There are many factors that affect the quality of silage and producers need to better understand these factors to optimise their silage making process. Our objective was to develop a web-based learning module to help users better understand the …

Canasta - Crop Niche Selection For Tropical Agriculture, A Spatial Decision Support System, R. O'Brien, M. Peters, R. Corner, S. Cook

IGC Proceedings (1977-2023)

Farmers in the developing world frequently find themselves in uncertain and risky environments, often having to make decisions based on very little information. Risks for smallholder farmers are often critical because of their poverty. In addition, in the tropics and subtropics, the natural environment is spatially and temporally variable and often harsh, thereby increasing the uncertainty faced by these farmers. This research aims to improve forage adoption decisions in the developing world, thereby increasing sustainable intensification and ultimately contributing to increased sustainable world food production and the alleviation of under-nutrition.

N-Mineralisation And Phosphorous: Important Elements In Decision Support For Grassland Systems, A. Lisbeth Nielsen, C. C. Hoffmann

IGC Proceedings (1977-2023)

Leaching of N and P from extensively managed grasslands on organic soils varies considerably. In environmentally sensitive areas it is important to diminish leaching by appropriate agricultural management. In Denmark low grazing intensity and management without fertilisation have been given a high priority. The type of soil has not been equally in focus, and it seems that the effect of cutting, compared to grazing, resulting in a higher removal of nutrients (e.g. Benke et al., 1992) can be used more strategically. The objective of this case study was to combine data from management strategies with data from leaching examinations …

Moving Lab-Based In-Person Training To Online Delivery: The Case Of A Continuing Engineering Education Program, Catherine Maware, David M. Parsley Ii, Kun Huang, Gerry M. Swan, Nelson Akafuah

Institute of Research for Technology Development Faculty Publications

Background: Online learning has recently surged due to the COVID-19 global pandemic. Because of the pandemic, many universities were forced to move to online learning, and various online teaching and learning environments emerged, both asynchronous and synchronous.

Objective: This study explores how a large university in the Southeastern United States of America converted an in-person Lean Manufacturing professional course into synchronous online learning for industry participants.

Method: The study analysed the performance of 212 in-person and 43 online industry participants and examined the views of online participants about the training. Paired t-tests, one-way multivariate analysis of covariance (MANCOVA), and univariate …

Activation Of The Renin–Angiotensin–Aldosterone System Is Attenuated In Hypertensive Compared With Normotensive Pregnancy, Robin C. Shoemaker, Marko Poglitsch, Hong Huang, Katherine Vignes, Aarthi Srinivasan, Cynthia Cockerham-Morris, Aric Schadler, John Anthony Bauer, John O'Brien

UK CARES Faculty Publications

Hypertension during pregnancy increases the risk of adverse maternal and fetal outcomes, but the mechanisms of pregnancy hypertension are not precisely understood. Elevated plasma renin activity and aldosterone concentrations play an important role in the normal physiologic adaptation to pregnancy. These effectors are reduced in patients with pregnancy hypertension, creating an opportunity to define the features of the renin–angiotensin–aldosterone system (RAAS) that are characteristic of this disorder. In the current study, we used a novel LC-MS/MS-based methodology to develop comprehensive profiles of RAAS peptides and effectors over gestation in a cohort of 74 pregnant women followed prospectively for the development …

Assessment Of Land And Renewable Energy Resource Potential For Regional Power System Integration With Ml Spatio-Temporal Clustering, Rosemary E. Alden, Claire Halloran, Donovin D. Lewis, Dan M. Ionel

Electrical and Computer Engineering Graduate Research

Toward the planning and development of future electric power systems with extremely large penetration of renewable resources (DERs), detailed assessment of power and energy potential is needed considering both spatial and temporal data per region. Within this paper, a methodology for spatio-temporal DER capacity potential considering land cover types and weather variation are presented using spatio-temporal data. Additionally, an application of empirical orthogonal functions (EOFs) and max-p unsupervised learning techniques is proposed for DER generation to identify zones of similar output power in space and time. A detailed case study for the example region of Kentucky, USA is completed with …

Fault Tolerant Electric Machine Concept For Aircraft Propulsion With Pm Rotor And Dc Current Stator Dual-Stage Excitation, Donovin D. Lewis, Oluwaseun A. Badewa, Ali Mohammadi, Matin Vatani, Dan M. Ionel

Electrical and Computer Engineering Graduate Research

The development of electrically propelled aircraft requires electric machines that are fault-tolerant, compact, and efficient at multiple operating points. This paper proposes a novel machine topology combining a coreless Halbach PM array axial flux unit and a PM-less synchronous hybrid excited radial flux unit. Both units are coupled on the same shaft to take advantage of the opportunities available in the unique usage profile for aviation. The machines can be used simultaneously for the take off and altitude climbing stage or used individually during the cruising stage. The coreless axial flux PM (CAFPM) motor unit consists of two Halbach array …