Digital Commons Network^™

Neuronal Cell Model And Methods Of Use Therefor, Craig S. Miller, Robert J. Danaher, Robert J. Jacob

Microbiology, Immunology and Molecular Genetics Faculty Patents

The present invention provides, inter alia, a PC12 cell quiescently infected with a neurotropic virus. Preferred quiescently-infected neural cells are those wherein the neurotropic virus is a neurotropic herpes virus. Methods related to these and other materials are also provided.

The Toxoplasma Gondii Protein Rop2 Mediates Host Organelle Association With The Parasitophorous Vacuole Membrane, Anthony P. Sinai, Keith A. Joiner

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Toxoplasma gondii replicates within a specialized vacuole surrounded by the parasitophorous vacuole membrane (PVM). The PVM forms intimate interactions with host mitochondria and endoplasmic reticulum (ER) in a process termed PVM–organelle association. In this study we identify a likely mediator of this process, the parasite protein ROP2. ROP2, which is localized to the PVM, is secreted from anterior organelles termed rhoptries during parasite invasion into host cells. The NH₂-terminal domain of ROP2 (ROP2hc) within the PVM is exposed to the host cell cytosol, and has characteristics of a mitochondrial targeting signal. In in vitro assays, ROP2hc is …

28th Annual Midwest/Midsouth Estate Planning Institute, Office Of Continuing Legal Education At The University Of Kentucky College Of Law

Continuing Legal Education Materials

Materials from the 28th Annual Midwest/Midsouth Estate Planning Institute held by UK/CLE in July 2001.

Evaluation Of Residual Chlorothalonil Levels On Processing Tomato Foliage Using The Tom-Cast Spray Program, Jane M. Patterson, Sue E. Nokes, Mark A. Bennett, Richard E. Riedel

Biosystems and Agricultural Engineering Faculty Publications

Field tomatoes were sprayed with chlorothalonil on a fixed–interval spray program and a TOM–CAST spray program with disease severity value threshold of 18. Foliage samples from upper and lower canopy layers were collected prior to spray re–applications. Chlorothalonil residue data were compared to the chlorothalonil efficacy threshold (1.2 μg/cm²). Using a seven–day interval program, eight of the nine and seven of the nine spray intervals had chlorothalonil residues above the critical level for the upper and lower canopy layers, respectively. Using the TOM–CAST program, four of the five spray intervals had chlorothalonil residues above the critical level for …

Influence Of Inoculum Size On Phytase Production And Growth In Solid-State Fermentation By Aspergillus Niger, Chundakkadu Krishna, Sue E. Nokes

Biosystems and Agricultural Engineering Faculty Publications

Solid–state fermentation is experiencing renewed interest for industrial enzyme production. Previous studies on the effects of fungal inoculum size on product yield have focused on spore inoculum. However, some organisms require vegetative inocula. This study investigated the effects of initial inoculum colony age, vegetative inoculum size, and duration of fermentation on the production of fungal biomass and phytase in solid–state fermentation using Aspergillus niger grown on wheat bran and soy meal. Initial inocula from 7– and 14–day–old potato dextrose agar (PDA) plates were used to study the effect of inoculum colony age in liquid culture and its further influence on …

Public Involvement In Highway Improvement: A Comparison Of Three Different Visualization Modes For A Case Study In Central Kentucky, Joel Brumm, Keiron Bailey, Ted H. Grossardt

Kentucky Transportation Center Faculty and Researcher Publications

Public involvement in highway improvement presents a set of complex problems involving many stakeholders.Since visualizations are increasingly regarded as essential tools in this process, a clearer understanding of the specific merits of advanced visualization techniques and their potential contributions must be developed.As a step towards integrating visualizations into an improved public planning paradigm termed Structured Public Involvement (SPI), this paper investigates the utility and performance of three visualization modes, termed 2D, 3D and VR (Virtual Reality), for a case study highway in Central Kentucky.

Visualization scenarios were designed and engineered according to the principle of elemental decomposition.Using a combination …

The Kentucky Plan: An Innovative Approach To Professional Doctoral Education In Public Health., Joel M. Lee, F. Douglas Scutchfield

Preventive Medicine and Environmental Health Faculty Publications

The Doctor of Public Health (Dr.P.H.) degree is designed to prepare graduates for the highest levels of leadership in public health related careers in both public and private settings. The Kentucky School of Public Health has developed an innovative generalist professional degree program offering an extensive battery of courses based on the body of professional public health knowledge. The degree includes extensive supervised field experience as part of the academic training, linking theory with practice. Issues related to professional degrees, curriculum, accreditation and certification, admission, evaluation, plans for institutional cooperation, and demand are addressed.

Tetrafluoroazidoaniline And Method Of Making And Using The Same, Hans Peter Spielmann, Kareem A.H. Chehade

Molecular and Cellular Biochemistry Faculty Patents

The invention is directed to a novel compounds comprising 4-azidotetrafluoroaniline and the alkyl, acyl and sulfonamide derivatives thereof and to methods of making and using the same. The novel compounds are useful as a photoaffinity probe to study protein structure and function. Two methods for preparing 4-azidotetrafluoroaniline are disclosed, each employing a stable carbamate intermediate from which the 4-azidotetrafluoroaniline is derived.

Isolated Spinach Ribulose-1,5-Bisphosphate Carboxylase/Oxgenase Large Subunit Ε N-Methyltransferase And Method Of Inactivating Ribulose-1,5-Bishosphatase Ε N-Methyltransferase Activity, Robert L. Houtz

Horticulture Faculty Patents

The gene sequence for ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) large subunit (LS)^εN-methyltansferase (protein methylase III or Rubisco LSMT) from a plant which has a des(methyl) lysyl residue in the LS is disclosed. In addition, the full-length cDNA clones for Rubisco LSMT are disclosed. Transgenic plants and methods of producing same which have the Rubisco LSMT gene inserted into the DNA are also provided. Further, methods of inactivating the enzymatic activity of Rubisco LSMT are also disclosed.

Adolescent And Young Adult Substance Use: Association With Sensation Seeking, Self Esteem And Retrospective Report Of Early Pubertal Onset. A Preliminary Examination, Catherine A. Martin, T. K. Logan, Carl Leukefeld, Rich Milich, Hatim Omar, Richard Clayton

Psychology Faculty Publications

Structured questionnaires were administered to investigate the relationship between early pubertal onset, substance abuse, sensation seeking, and self-esteem. The current study presents data from 1,002 subjects, who were followed from the 6th to the 10th grades and again at the age of 20. In females, early pubertal onset was associated with greater cigarette use and lower self-esteem. Further the interaction of early pubertal onset and low self-esteem was predictive for cigarette use in females, as was early pubertal onset and high sensation seeking. Late pubertal onset was associated with decreased alcohol use and lower sensation seeking in males, with the …

Comparison Of The Quantification Of Caffeine In Human Plasma By Gas Chromatography And Elisa, A. B. Carregaro, W. E. Woods, Thomas Tobin, A. Queiroz-Neto

Veterinary Science Faculty Publications

In the present study we evaluated the precision of the ELISA method to quantify caffeine in human plasma and compared the results with those obtained by gas chromatography. A total of 58 samples were analyzed by gas chromatography using a nitrogen-phosphorus detector and routine techniques. For the ELISA test, the samples were diluted to obtain a concentration corresponding to 50% of the absorbance of the standard curve. To determine whether the proximity between the I₅₀ of the standard curve and that of the sample would bring about a more precise result, the samples were divided into three blocks according …

Pride Water Quality Assessment Report: Iv. Nutrient Assessment, Kentucky Water Resources Research Institute, University Of Kentucky

KWRRI Research Reports

The efficient utilization of federal funds in improving the water quality and aquatic habitat of the region requires a mechanism for assessing and evaluating the impacts of the proposed and ongoing projects as well as some mechanism for prioritizing the allocation of additional funds. In order to evaluate the effectiveness of these projects it is important to provide a formal monitoring and assessment program based on sound scientific principles. This report provides an initial 10 year baseline assessment of the existing water quality conditions in the 40 county PRIDE region for the purpose of evaluating the impacts of the PRIDE …

Use Of The Naturally-Occuring Quinones Thymoquinone And Dithymoquinone As Antineoplastic And Cytotoxic Agents, Peter A. Crooks, David R. Worthen, Omar A. Ghosheh

Pharmaceutical Sciences Faculty Patents

Nigella sativa derivatives, thymoquinone (TM) and dithymoquinone (DIM) are used in treatment of parental and multi-drug resistant human cancers.

The Many Inventions Of Childhood: A Tentative Taxonomy, Leonora Ritter

disClosure: A Journal of Social Theory

No abstract provided.

Book Review: Cyborg Babies: From Techno-Sex To Techno-Tots. Robbie Davis-Floyd And Joseph Dumit, Eds. New York: Routledge, 1998., Tobie Saad

disClosure: A Journal of Social Theory

No abstract provided.

Capturing The Child: Gothic Subject Categories And Erotic Tropes Of Appeal. Disclosure Interviews James Kincaid, Matthew Renfro-Sargent, Tobie Saad

disClosure: A Journal of Social Theory

No abstract provided.

Childhood And Child Life. Disclosure Interviews Jo Boyden, Margo Kleinfeld

disClosure: A Journal of Social Theory

No abstract provided.

Aluminium Toxicokinetics: An Updated Minireview, Robert A. Yokel, Patrick J. Mcnamara

Pharmaceutical Sciences Faculty Publications

This MiniReview updates and expands the MiniReview of aluminium toxicokinetics by Wilhelm et al. published by this journal in 1990. The use of ²⁶Al, analyzed by accelerator mass spectrometry, now enables determination of Al toxicokinetics under physiological conditions. There is concern about aluminium in drinking water. The common sources of aluminium for man are reviewed. Oral Al bioavailability from water appears to be about 0.3%. Food is the primary common source. Al bioavailability from food has not been adequately determined. Industrial and medicinal exposure, and perhaps antiperspirant use, can significantly increase absorbed aluminium. Inhalation bioavailability of airborne soluble Al …

Estrogen Protects Against The Synergistic Toxicity By Hiv Proteins, Methamphetamine And Cocaine, Jadwiga Turchan, Caroline Anderson, Kurt F. Hauser, Qinmiao Sun, Jiayou Zhang, Ying Liu, Phyllis M. Wise, Inna Kruman, William Maragos, Mark P. Mattson, Rosemarie Booze, Avindra Nath

Neurology Faculty Publications

BACKGROUND: Human immunodeficiency virus (HIV) infection continues to increase at alarming rates in drug abusers, especially in women. Drugs of abuse can cause long-lasting damage to the brain and HIV infection frequently leads to a dementing illness. To determine how these drugs interact with HIV to cause CNS damage, we used an in vitro human neuronal culture characterized for the presence of dopaminergic receptors, transporters and estrogen receptors. We determined the combined effects of dopaminergic drugs, methamphetamine, or cocaine with neurotoxic HIV proteins, gp120 and Tat.

RESULTS: Acute exposure to these substances resulted in synergistic neurotoxic responses as measured by …

Enhanced Expression Of Human Platelet-Derived Growth Factor In Pichia Pastoris, David M. Kaetzel

Pharmacology and Nutritional Sciences Faculty Patents

Enhanced yields of the platelet-derived growth factor (PDGF) B-chain are obtained using the Pichia pastoris yeast system. Yields of both wild-type and mutant human proteins are enhanced when the yeast is transformed with a vector containing the yeast mating factor promoter fused to a sequence encoding the mature protein. The secreted wild-type protein is indistinguishable from mature 29-32 kDa protein isolated from human and Chinese hamster ovary (CHO) cells. An RKK→EEE mutant exhibited reduced association with the cell surface and accumulated in the culture medium as 29-32 kDa forms. Stable transfection of U87 astrocytoma cells with RKK→EEE mutants of either …

Am-Pm Cutting Of Alfalfa, Garry D. Lacefield, Michael Collins, Jimmy C. Henning

Kentucky Alfalfa and Stored Forage Conference

Over the past twenty years of this Conference we have spent considerable time discussing "Alfalfa Harvest Management." We have discussed the importance of stage of maturity and its impact on quality on many occasions. We have presented research on many aspects of hay and haylage management. An overall theme for all the discussions has been "harvest for quality," with emphasis on saving the leaves during the entire harvesting process. Our recommendations have, are, and will continue to be centered around those management practices that will result in the greatest opportunity for preserving alfalfa quality from standing crop to feeding. This …

Hormonal And Barrier Methods Of Contraception, Oncogenic Human Papillomaviruses, And Cervical Squamous Intraepithelial Lesion Development, Ann L. Coker, Laura C. Sanders, Sharon M. Bond, Tsilya Gerasimova, Lucia Pirisi

CRVAW Faculty Journal Articles

We assessed the influence of hormonal (oral, injectable, or levonorgestrel [Norplant, Wyeth-Ayerst, Philadelphia, PA]) and barrier methods of contraception on the risk of cervical squamous intraepithelial lesions (SIL), while adjusting for high-risk (HR) HPV infection. Subjects were women receiving family planning services through the state health department clinics from 1995 to 1998. We selected 60 cases with high-grade cervical/SIL (HSIL) and 316 with low-grade cervical/SIL (LSIL) and controls (427 women with normal cervical cytology) and analyzed cervical DNA for HR-HPV, using Hybrid Capture I (Digene; Gaithersburg, MD).When assessing ever use, duration, recency, latency, and age at first use, neither oral …

Impact Of Gravel Dredging Operations On Surface Water Quality In Streams In The Upper Cumberland Basin, Jeffrey D. Jack, Arthur C. Parola, Anna L. Curini, Randall Kelley, Mark N. French, D. J. Hagerty, William M. Sampson

KWRRI Research Reports

This is a report to the USEPA, Kentucky Division of Water and the Kentucky Water Resources Research Institute, focused on the biologic and morphological impacts of gravel mining in the upper Cumberland basin.

Kentucky Water Resources Research Institute Annual Technical Report Fy 2000, Kentucky Water Resources Research Institute, University Of Kentucky

KWRRI Annual Technical Reports (USGS’s 104b Grant Program)

The FY 2000 Annual Technical Report for Kentucky consolidates the reporting requirements of the Section 104(b) base grant and previous regional competitive grant awards in a single technical report that includes: 1) a synopsis of each ongoing research project and each project completed during the period, 2) a list of related reports published, 3) a description of information transfer activities, 4) a summary of student support during the reporting period, and 5) notable achievements and awards during the year.

Designing Molecular Recognition In The Context Of Hydrogen Bonding And Molecular Dynamics, Peter G. Willis

University of Kentucky Doctoral Dissertations

The effect of hydrogen bonding on the conformation of organic moleculesunifies two projects in this thesis. In one project, the stability of the intramolecularhydrogen bond in derivatives of 2-guanidinobenzimidazole was studied bydynamic 1H NMR spectrometry. The impact that this intramolecular hydrogenbond had on the bond order of the neutral guanidino group and on the dynamicconformation of these aromatic structures was related to the concept of hydrogenbond-assisted resonance. In another project, an oligomer possessing repetitiveconformation and capable of much inter- and intramolecular hydrogen bondingwas designed and synthesized. The sensitivity of this oligomer to changes inanion concentration, as well as its own …

Characterization Of Epitheliogenesis Imperfecta In Equus Caballus, Louis D. Lieto

University of Kentucky Doctoral Dissertations

Epitheliogenesis Imperfecta (EI) is a mechanobullous disease that occurs in newborn American Saddlebred and Belgian Draft foals. Necropsy evaluations of two American Saddlebred foals revealed broad skin lesions, dental abnormalities and oral mucosa lesions. Construction of a partial pedigree showing occurrences of EI in American Saddlebred horses was consistent with a recessive pattern of inheritance. An allelic frequency of 0.04 was estimated for the EI gene. The pathological signs of EI were similar to a disease in humans known as Herlitz Junctional Epidermolysis Bullosa (HJEB). HJEB is caused by a defect in one of the three subunits of the laminin …

A "Clanging Silence": Same-Sex Couples And Tort Law, John G. Culhane

Kentucky Law Journal

No abstract provided.

Romer, Hurley, And Dale: How The Supreme Court Languishes With "Special Rights", Christopher S. Hargis

Kentucky Law Journal

No abstract provided.

A Family Affair: Constitutional And Prudential Interests Implicated When Homosexuals Seek To Preserve Or Create Parent-Child Relationships, Kif Skidmore

Kentucky Law Journal

No abstract provided.

Pharmaceutical Patent Protection: More Generic Favored Legislation May Cause Pioneer Drug Companies To Pull The Plug On Innovation, Mandy Wilson

Kentucky Law Journal

No abstract provided.