Digital Commons Network^™

Complete Issue Of Volume 1

Kaleidoscope

No abstract provided.

Full Integration Of Undergraduates Into Laboratory Research, David A. Atwood, Aaron Hutchison

Kaleidoscope

No abstract provided.

Undergraduate Awards And Honors, Uk Office Of Undergraduate Research

Kaleidoscope

No abstract provided.

Information Based Regulation And International Trade In Genetically Modified Agricultural Products: An Evaluation Of The Cartagena Protocol On Biosafety, Michael P. Healy

Law Faculty Scholarly Articles

This Article considers the regulation of international trade in genetically modified agricultural products. Specifically, it addresses both products released into the environment as seeds and products intended for consumption as food. The first part of the Article describes the significance of genetically modified organisms (GMOs) in modem agriculture, especially agriculture in the United States. This discussion summarizes the risks and potential benefits associated with the use of agricultural GMOs, especially the risks and benefits related to biodiversity. The Article then briefly describes the approaches to the regulation of these products adopted in the

Cartagena Protocol to the Convention on Biological …

Oswald Research And Creativity Program, Uk Office Of Undergraduate Research

Kaleidoscope

No abstract provided.

Modulation Of High Affinity Hormone Binding To Lh/Cg Receptor, Meena Sundaramoorthy

University of Kentucky Master's Theses

Precise control of physiological phenomena is performed by various kinds of receptormediated signaling. The vast majority of receptors belong to the superfamily of G proteincoupled receptors (GPCRs), which form one of the largest protein families. In theclassical model of GPCR signaling, stimulation of seven transmembrane spanning GPCRleads to the activation of heterotrimeric G proteins, which dissociate into a and bgsubunits. The subunits activate effector molecules, which include second messengergenerating systems, giving rise to various kinds of cellular responses. The LH/CGreceptor is a member of the glycoprotein hormone receptor family along with the FSHand TSH receptors, which belongs to the GPCR …

The Jak-Stat Pathway Is Required For Multiple Early Events In Drosophila Oogenesis, Jennifer Renee Matlock

University of Kentucky Master's Theses

The Janus kinase (JAK) pathway is an integral part of signaling through a variety of ligands and receptors in mammals. The extensive reutilization and pleiotropy of this pathway in vertebrate development is conserved in other animals as well. In Drosophila melanogaster, JAK signaling is involved in embryonic pattern formation, sex determination, larval blood cell development, wing venation, planar polarity in the eye, and formation of other adult structures. Here we describe several roles for JAK signaling in Drosophila oogenesis. The gene for a JAK pathway ligand, unpaired, is expressed specifically in the polar follicle cells, two pairs of somatic cells …

The Efficiacy Of Various Salmonella Intervention Methods Applied To Pork Carcasses During Slaughter, Nathan C. Clayton

University of Kentucky Master's Theses

Twenty-one market-age swine slaughtered in the University of Kentuckyabattoir were inoculated with fecal slurry containing two strains of nalidixic acidresistant Salmonella typhimurium on the ham, belly, and jowl regions on eachside of the carcass. Trial 1 revealed that a 10 s hot water spray was just aseffective as the 20 s spray in removing S. typhimurium, as there was nodistinguishable difference in population. The shorter flame singe (10 s) was aseffective as the 20 s application and the two chlorine solutions (100, 200 ppm)had similar results. The 2% lactic acid spray reduced S. typhimurium populationssignificantly more than the 1% treatment. …

Compositional Perspectives On The Exchange Of Muna Slate Wares In The Late And Terminal Classic Northern Maya Lowlands, Christopher M. Gunn

University of Kentucky Master's Theses

This thesis presents the results of petrographic point counting analyses of Muna Slate ware, thepredominant slipped ceramic ware in the Northern Maya Lowlands during the Late and TerminalClassic Periods (600-1000 A.D.) of Maya prehistory. Recently, it was hypothesized that MunaSlate wares were centrally produced and distributed from the Puuc Hills site of Sayil (Smyth andDore 1994; Smyth et al. 1995). Given that Muna Slate wares may be considered utilitariansubsistence items (sensu Brumfiel and Earle 1987), this suggestion runs counter to severalarguments that ancient Maya utilitarian ceramics production is associated with outlyingcommunities and that their distribution is localized. In the research …

When May A State Require Teaching Alternatives To The Theory Of Evolution? Intelligent Design As A Test Case, Stephen L. Marshall

Kentucky Law Journal

No abstract provided.

Conceptualizing The Field After September 11th: Foreword To A Symposium On Public Health Law, Lawrence O. Gostin

Kentucky Law Journal

No abstract provided.

School Vaccination Requirements: Historical, Social, And Legal Perspectives, James G. Hodge Jr., Lawrence O. Gostin

Kentucky Law Journal

No abstract provided.

Public Health Law: Power, Duty, Restraint By Lawrence O. Gostin, Julia Field Costich

Kentucky Law Journal

No abstract provided.

The Organ Trail: Express Versus Presumed Consent As Paths To Blaze In Solving A Critical Shortage, Emily Denham Morris

Kentucky Law Journal

No abstract provided.

The Naked Clone, John Charles Kunich

Kentucky Law Journal

No abstract provided.

Cognitive And Somatic Item Response Pattern Of Orofacial Pain Patients Compared To Fibromyalgia Patients And A Non-Pain Control Group, Morten Hadsel

University of Kentucky Master's Theses

Previous work has suggested that chronic pain patients report psychological distress through higher endorsement of somatic rather than cognitive signs of anxiety and depression. The present study compared female Non-Pain (11=52), Orofacial Pain (n=3 17) and Fibromyalgia (n=50) groups. on SCL-90-R Somatization, Anxiety and Depression raw scores and cognitive-somatic symptom patterning of the Anxiety and Depression scales. Comparisons were also made amongst orofacial pain diagnostic subgroups and subgroups based on Multidimensional Pain Inventory (MPI) classification groups. The Somatization. Anxiety and Depression scores were higher in the OroFacial Pain and Fibromyalgia than Non-Pain group and higher in the Dysfunctional than Adaptive …

The Place Of Dietary Practices In The Lives Of Older Women, Lisa Marie Curch

University of Kentucky Doctoral Dissertations

Studies have supported the benefits of positive dietary behaviors in preventing or reducing morbidity and extending longevity, as well as the psychosocial function of dietary practices for quality of life. Research is needed though on the dietary behaviors of elders in general and older women in particular, for whom gender affects lifelong dietary patterns. Health behavior theory has typically focused on psychological factors, to the neglect of sociocultural processes. This investigation utilized a life course perspective, enhanced by social interactionist elements, to address aspects of development and change in behavior neglected by health behavior theories, such as temporal dimensions and …

Structure And Function Analysis Of Par-4, Nadia M. El-Guendy

University of Kentucky Doctoral Dissertations

Par-4 is a leucine zipper domain protein that induces apoptosis on its own in certain cancer cells and in Ras-transformed cells, but not in normal or immortalized cells. Par-4 induces apoptosis by activation of the Fas death receptor pathway and co-parallel inhibition of NF-B transcription activity. Cells that are resistant to apoptosis by Par-4 alone, however, are greatly sensitized by Par-4 to the action of other pro-apoptotic insults such as growth factor withdrawal, TNF, ionizing radiation, intracellular calcium elevation, or those involved in neuronal degeneration such as Alzheimer's, Parkinson's, Huntington's and Stroke. Previous studies have suggested that the apoptosis-sensitization potential …

Creation Of A Bacterial Mutagenicity Assay Highly Sensitive To Dialkylnitrosamines, Matthew Troy Cooper

University of Kentucky Doctoral Dissertations

Although dialkylnitrosamines are environmentally significant carcinogens, the use of short-term bioassays to assess the mutagenic potential of these compounds remains problematic. The Ames test, a mutagenicity assay based on the reversion of Salmonella typhimurium histidine auxotrophs, is the most widely used bioassay in genetic toxicology, but the traditional Ames tester strains are largely insensitive to dialkylnitrosamine mutagenicity. I have constructed several mutagenicity tester strains that co-express combinations of full-length human cytochrome P450 2E1, rat cytochrome P450 reductase, and human cytochrome b5 in S. typhimurium lacking ogt and ada methyltransferases (YG7104ER, ogt-; and YG7108ER, ogt-, ada-). These new strains are susceptible …

Roles Of The Jak Pathway In Follicular Patterning In Drosophila, Rongwen Xi

University of Kentucky Doctoral Dissertations

The JAK-STAT pathway is an intracellular signaling pathway that is found to have crucial roles in hematopoiesis, immune response and the development of many other tissues in mammals. The pathway is conserved in Drosophila melanogaster, and is much simpler: there is only one Drosophila JAK (Hopscotch, Hop) and STAT (STAT92E) respectively, while there are at least 4 JAKs and 7 STATs in mammals. The pathway has been intensively studied in Drosophila, and has been implicated in many tissue development and cellular processes. In this work, I present several roles of JAK signaling in oogenesis.First, JAK signaling is required for cell …

Identification And Characterization Of Contact Sites Between Human Chorionic Gonadotropin And The Amino Terminal Region Of The Luteinizing Hormone/Choriogonadotropin Receptor, Rebecca Mccaffrey

University of Kentucky Master's Theses

The luteinizing hormone / choriogonadotropin receptor (LH/CG-R) is a member of theG protein-coupled receptor family. The LH/CG-R has seven transmembrane helices, threeexoloops, three cytoloops, a C-terminal tail, and an extensive N-terminal exodomain. Theexodomain is capable of binding hormone with high affinity without hormone action. Previousstudies have shown that the amino-terminal region of the LH/CG receptor contacts both subunitsof human chorionic gonadotropin (hCG). In particular, three residues (Leu20, Cys22, and Gly24)were found to be crucial for hormone binding. In this thesis work, benzoylphenylalanine (Bpa),a photoactivatable reagent, was used to continue investigating the interactions of the N-terminalregion of the LH/CG-R with hCG. …

Interacting Color And Behavior Responses To Multiple Selection Pressures In The Sister Salamander Species Ambystoma Barbouri And Ambystoma Texanum., Tiffany Sacra Garcia

University of Kentucky Doctoral Dissertations

My research explores the complex strategies animals adapt to cope with multiple selection pressures. I studied the behavioral and color response of two salamander sister species, Ambystoma barbouri and A. texanum, to temperature, predation risk and ultraviolet radiation (UVR, 280-320 nm). Ambystoma barbouri undergo development in streams, while A. texanum larvae inhabit ponds. Thus, A. barbouri are exposed to increased habitat ephemerality, enhanced predation risk, and UVR exposure. I show how A. barbouri have evolved alternate coping mechanisms in response to these environmental factors, relative to A. texanum. In this comparison study, I've quantified the affects of these selection pressures …

Genes Encoding Several Poly (Adp-Ribose) Glycohydrolase (Parg) Enzymes, The Proteins And Fragments Thereof, And Antibodies Immnoreactive Therewith, Myron Jacobson, Elaine L. Jacobson, Jean-Christoph Amé, Winston Lin

Graduate Center for Nutritional Sciences Faculty Patents

The isolation and characterization of cDNAs encoding poly(ADP-ribose) glycohydrolase (PARG) enzymes and the amino acid sequences of PARGs from several species are described. PARG is involved in the cellular response to DNA damage and its proper function is associated with the body's response to neoplastic disorder inducing agents and oxidative stress. Expression vectors containing the cDNAs and cells transformed with the vectors are described. Probes and primers that hybridize with the cDNAs are described. Expression of the cDNA in E. coli results in an enzymatically active protein of about 111 kDa and an active fragment of about 59 kDa. Methods …

Protection Against Ischemia And Reperfusion Injury, Peter R. Oeltgen, Paul D. Bishop, Mark S. Kindy, Juan A. Sanchez

Molecular and Cellular Biochemistry Faculty Patents

A compound and method for using the compound to reduce injury associated with ischemia and reperfusion of mammalian organs such as the heart. The compound may be administered as part of a preconditioning strategy which reduces the extent of injury and improves organ function following cessation and restoration of blood flow. The compound may be used in preparation for planned ischemia or in a prophylactic manner in anticipation of further ischemic events.

Drug Delivery System Involving Interaction Between Protein Or Polypeptide And Hydrophobic Biodegradable Polymer, Patrick P. Deluca

Pharmaceutical Sciences Faculty Patents

A drug delivery system for controlled release of a protein or polypeptide comprising a hydrophobic biodegradable polymer and a protein or polypeptide. A physical interaction is present between the polymer and the protein or polypeptide, thus, allowing protection and controlled release of the protein or polypeptide in-vivo. The drug delivery system may be prepared by a polymer precipitation technique or a microsphere technique.

Cheese Making With Bacteriophage Resistant Bacteria, Clair D. Hicks

Animal and Food Sciences Faculty Patents

A method is provided for reducing or preventing bacteriophage attack on bacteria used in a cheese making process. The method includes (a) treating a blocker peptide precursor with a protease enzyme that hydrolyzes the precursor to produce blocker peptides; (b) collecting the blocker peptides so produced; (c) formulating a starter media with the blocker peptides; (d) growing bulk cultures of cheese making bacteria in the inoculated starter media; and (e) adding bacteria grown in the inoculated starter media to a fermentation medium for producing cheese. The present invention also includes a method of making cheese and cheese produced by the …

Water Soluble Derivatives Of Camptothecin/Homocamptothecin, Thomas Burke, Ayhan Demir, Cihangir Tanyeli, Ashok J. Chavan, Tie-Lin Wang, Yves Pommier

Pharmaceutical Sciences Faculty Patents

Camptothecin and homocamptothecin analogs and derivatives are provided incorporating alkylamine and polyalkylamine moieties.

Farnesyl Pyrophosphate Analogs, Hans Peter Spielmann, Douglas A. Andres, Kareem A.H. Chehade

Molecular and Cellular Biochemistry Faculty Patents

The post-translational addition of a farnesyl moiety to the Ras oncoprotein is essential for its membrane localization and is required for both its biological activity and ability to induce malignant transformation. The present invention describes design and synthesis of a farnesylpyrophosphate (FPP) analog, 8-anilinogeranyl pyrophosphate (AGPP) that is transferred to Ras by farnesyltransferase (FTase), in which the ω-terminal isoprene unit of the farnesyl group has been replaced with an aniline functionality. AGPP potently inhibited FTase activity in vitro (IC₅₀=0.6 μM) and is highly selective showing little inhibitory activity against either geranylgeranyl-protein transferase type I (GGTase I) (IC_{50 …}

Textualism’S Limits On The Administrative State: Of Isolated Waters, Barking Dogs, And Chevron, Michael P. Healy

Law Faculty Scholarly Articles

In Solid Waste Agency of Northern Cook County (SWANCC) v. U.S. Army Corps of Engineers, the U.S. Supreme Court recently held that the U.S. Army Corps of Engineers (the Corps) does not have authority under the Clean Water Act (the Act or the CWA) to regulate the filling of “other waters.” This decision demonstrates a major shift in the Court's approach to statutory interpretation, particularly in the context of reviewing an agency’s understanding of a statute. The significance of the case is best gauged by contrasting it with United States v. Riverside Bayview Homes, Inc. There, the Court, acting …

Tractor Driving Among Kentucky Farm Youth: Results From The Farm Family Health And Hazard Surveillance Project, Steven R. Browning, Susan C. Westneat, Raeanne Szeluga

Epidemiology and Environmental Health Faculty Publications

This article documents the extent of children's involvement in tractor operations among a representative sample of Kentucky children living and working on family farms. Specifically, we describe children's exposures to tractor-related work activities, profile their use of the tractor (number of days worked), and assess compliance with generally recommended safety measures, such as using tractors equipped with ROPS (rollover protective structures), avoiding riding as passengers on tractors, and operating tractors on public roadways. Data for this study were collected in 1994 and 1995 as part of the NIOSH-sponsored Farm Family Health and Hazard Surveillance Project (FFHHSP). Despite recognition in the …