Digital Commons Network^™

Mechanical Properties Of Corn And Soybean Meal, Marek Molenda, Michael D. Montross, Jozef Horabik, Ira Joseph Ross

Biosystems and Agricultural Engineering Faculty Publications

Ground corn and soybean meal are common ingredients in feed mixes. The knowledge of their mechanical properties is important to the feed manufacturer and consumer. Changes in these properties can lead to abnormally high or low levels of active ingredients in finished feed, thus decreasing its quality. Mechanical properties of wheat, corn meal, and soybean meal were investigated using a modified direct shear apparatus. The moisture content (wet basis), uncompacted bulk density, and particle density were: 10.4%, 733 kg/m³, and 1410 kg/m³ for soft red winter wheat; 11.4%, 583 kg/m³, and 1350 kg/m³ for …

Methods Of Making Modified Polypeptides, Joseph Chappell, Kathleen R. Manna, Joseph P. Noel, Courtney M. Starks

Pharmaceutical Sciences Faculty Patents

Novel polypeptides and the corresponding nucleic acids encoding such polypeptides are disclosed herein. The invention provides methods of making modified polypeptides by altering one or more amino acid residues involved in the active site of a preselected polypeptide.

Topical Micronutrient Delivery System And Uses Of Thereof, Elaine L. Jacobson, Myron Jacobson, Jaber G. Qasem, Hyuntae Kim, Moonsun Kim

Graduate Center for Nutritional Sciences Faculty Patents

The invention involves methods and compositions useful in delivering micronutrients to cells. By formulating the micronutrient in the form of an ester that is convertible to the active form of the micronutrient, one can combine it with a co-ester that inhibits esterases, so that the micronutrient can reach the targeted cells prior to degradation. Both methods and compositions are described.

Regulation Of Lipoprotein Lipase By Protein Kinase Cα In 3t3-F442a Adipocytes, Gouri Ranganathan, Wei Song, Nicholas Dean, Brett Monia, Steven W. Barger, Philip A. Kern

Clinical and Translational Science Faculty Publications

Lipoprotein lipase (LPL) is an important enzyme in adipocyte and lipid metabolism with complex cellular regulation. Previous studies demonstrated an inhibition of LPL activity and synthesis following depletion of protein kinase C (PKC) isoforms with long term treatment of 3T3-F442A adipocytes with 12-O-tetradecanoylphorbol-13-acetate. To identify the specific PKC isoforms involved, we treated cells with antisense oligonucleotides that block expression of specific PKC isoforms. An antisense oligonucleotide to PKCα inhibited LPL activity by 78 ± 8%, whereas antisense oligonucleotides directed against PKCδ or PKCε had no effect on LPL activity. The change in LPL activity was maximal at 72 …

Compounds Encoding The Protective M-Like Protein Of Streptococcus Equi And Assays Therefor, John F. Timoney, Sergey Artiushin

Veterinary Science Faculty Patents

The present invention relates generally to molecular compounds which encode the protective M-like protein of Streptococcus equi (SeM), the amino acid compound which is thereby encoded, and compositions of matter which incorporate either the encoding compounds or the cellular components for which they encode. For instance, vaccines which utilize the amino acid compounds or vectors and cell lines useful to make the amino acid compounds described herein are subjects of the present invention. The present invention provides methods to stimulate S. equi-specific immune response in horses. It also provides diagnostic assays for Streptococcus equi.

Brain Uptake, Retention, And Efflux Of Aluminum And Manganese, Robert A. Yokel

Pharmaceutical Sciences Faculty Publications

My colleagues and I investigated the sites and mechanisms of aluminum (Al) and manganese (Mn) distribution through the blood-brain barrier (BBB). Microdialysis was used to sample non-protein-bound Al in the extracellular fluid (ECF) of blood (plasma) and brain. Brain ECF Al appearance after intravenous Al citrate injection was too rapid to attribute to diffusion or to transferrin-receptor-mediated endocytosis, suggesting another carrier-mediated process. The brain:blood ECF Al concentration ratio was 0.15 at constant blood and brain ECF Al concentrations, suggesting carrier-mediated brain Al efflux. Pharmacological manipulations suggested the efflux carrier might be a monocarboxylate transporter (MCT). However, the lack of Al …

Development Of A Finite-Element Stored Grain Ecosystem Model, Michael D. Montross, Dirk E. Maier, Kamyar Haghighi

Biosystems and Agricultural Engineering Faculty Publications

An axisymmetric finite–element model was developed that predicts the heat, mass, and momentum transfer that occurred in upright corrugated steel storage structures due to conduction, diffusion, and natural convection using realistic boundary conditions. Weather data that included hourly total solar radiation, wind speed, ambient temperature, and relative humidity were used to model the temperature, moisture content, dry matter loss, and maize weevil development during storage with no aeration, and with ambient and chilled aeration. Periods of aeration were simulated assuming a uniform airflow rate through the grain mass. Heat and mass balances were used to calculate the temperature and absolute …

Validation Of A Finite-Element Stored Grain Ecosystem Model, Michael D. Montross, Dirk E. Maier, Kamyar Haghighi

Biosystems and Agricultural Engineering Faculty Publications

An axisymmetric finite–element model was validated with respect to predicting the heat, mass, and momentum transfer that occurred in upright corrugated–steel storage bins due to conduction, diffusion, and natural convection using realistic boundary conditions. Hourly weather data that included hourly total solar radiation, wind speed, ambient temperature, and relative humidity were used to model the corn temperature and moisture content during storage with no aeration, and with ambient and chilled aeration. Periods of aeration were simulated assuming a uniform airflow rate through the grain mass. Sixteen bins with a capacity of 11.7 t each and instrumented with temperature cables were …

Defective Cd19-Dependent Signaling In B-1a And B-1b B Lymphocyte Subpopulations., Goutam Sen, Hsin-Jung Wu, Gabriel Bikah, Chandrasekar Venkataraman, Darrell A Robertson, E Charles Snow, Subbarao Bondada

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Peritoneal and pleural cavities in mice and humans contain a unique population of B-lymphocytes called B-1 cells that are defective in B cell antigen receptor (BCR) signaling but have an increased propensity to produce autoantibodies. Several molecules such as Btk, Vav, and CD19 known to be important for BCR signaling have been shown to be critical for the development of B-1 cells from undefined precursors. Here we demonstrate that B-1 cell unresponsiveness to BCR cross-linking is in part due to defective signaling through CD19, a molecule known to modulate signaling thresholds in B cells. The defective CD19 signaling is manifested …

Soy Products Having Improved Odor And Flavor And Methods Related Thereto, William L. Boatright

Animal and Food Sciences Faculty Patents

The present invention provides improved soy protein products, soy plants and seeds, and methods to produce the soy protein products.

Water Soluble Amine Esters Of Testosterone For Intranasal Administration, Anwar A. Hussain

Pharmaceutical Sciences Faculty Patents

The present invention provides novel water soluble testosterone analogs. These testosterone analogs are suitable for intranasal administration to patients requiring increased plasma testosterone levels. The present invention also provides pharmaceutical compositions containing the testosterone analogs of the present invention. The present invention further provides a method of increasing plasma testosterone levels in patients in need of such treatment comprising the intranasal administration of the water-soluble testosterone analogs and pharmaceutical compositions of the present invention.

Method For Analyzing Molecular Expression Or Function In An Intact Single Cell, Philip W. Landfield, Olivier Thibault, Eric M. Blalock, Kuey-Chu Chen, Patrick Kaminker

Pharmacology and Nutritional Sciences Faculty Patents

A method for obtaining molecules from a substantially intact single cell is disclosed. The method differs from prior methods that obtain a variable fraction of a cell's contents and therefore cannot quantitatively estimate the number of molecules in the cells. The present method comprises isolating and harvesting a substantially intact single cell from its organ tissue comprising the steps of subjecting a tissue mass to a dissociation method so that the cells are dissociated from the tissue to expose cell bodies or cell processes, contacting a dissociated cell with a device capable of collecting the cell from the tissue substantially …

Pumping Or Mixing System Using A Levitating Magnetic Element, Aexandre N. Terentiev

Physics and Astronomy Faculty Patents

A system capable of pumping or mixing relatively warm fluids using a rotating magnetic element or bearing levitated by a cold superconducting element is disclosed. The magnetic element or bearing carries at least one impeller and is placed in a fluid vessel positioned external to the outer wall of a cryostat or other housing for the superconducting element. A separate cooling source thermally linked to the superconducting element provides the necessary cooling to create the desired superconductive effects and induce levitation in the magnetic element or bearing. The outer wall or housing defines a chamber around the cold superconducting element …

Method And Use Of Α-Amino-Β-Mercapto-Ethane Derivatives As Dicarbonyl Scavengers For Treatment Of Conditions Resulting From Protein, Lipid, And Dna Damage, Elaine L. Jacobson, Myron Jacobson, Georg T. Wondrak, Daniel Cervantes Laurean

Graduate Center for Nutritional Sciences Faculty Patents

Methods of inhibiting damage to proteins, lipids, and DNA by the use of penicillamines and other α-amino-β,β-mercapto-β,β-dimethyl-ethane compounds as dicarbonyl scavengers is disclosed.

Spin Trapping Pharmaceutical Compositions And Methods For Use Thereof, John M. Carney, Robert A. Floyd

Pharmacology and Nutritional Sciences Faculty Patents

Spin trapping compositions in general have now been discovered to be effective in treating a variety of disorders, including disorders such as those arising from ischemia, infection, inflammation, exposure to radiation or cytotoxic compounds, not just of the central and peripheral nervous systems but of peripheral organ disease having a wide variety of etiologies. In the preferred embodiment, the compositions for treating tissue damage from ischemia contain PBN, or active derivatives thereof, in a suitable pharmaceutical carrier for intravenous, oral, topical, or nasal/pulmonary administration. Other preferred spin-trapping agents include 5,5-dimethyl pyrroline N-oxide (DMPO), α-(4-pyridyl-1-oxide)-N-tert-butylnitrone (POBN), and (TEMPO) and spin-trapping derivatives …

Genes Encoding Several Poly (Adp-Ribose) Glycohydrolase (Parg) Enzymes, The Proteins And Fragments Thereof, And Antibodies Immnoreactive Therewith, Myron Jacobson, Elaine L. Jacobson, Jean-Christoph Amé, Winston Lin

Graduate Center for Nutritional Sciences Faculty Patents

The isolation and characterization of cDNAs encoding poly(ADP-ribose) glycohydrolase (PARG) enzymes and the amino acid sequences of PARGs from several species are described. PARG is involved in the cellular response to DNA damage and its proper function is associated with the body's response to neoplastic disorder inducing agents and oxidative stress. Expression vectors containing the cDNAs and cells transformed with the vectors are described. Probes and primers that hybridize with the cDNAs are described. Expression of the cDNA in E. coli results in an enzymatically active protein of about 111 kDa and an active fragment of about 59 kDa. Methods …

Bidirectional Promoters And Methods Related Thereto, Sushen Gan, Mingtang Xie, Yuehui He

Kentucky Tobacco Research and Development Center Faculty Patents

The present invention comprises artificial nucleic acid constructs comprising a bidirectional promoter having minimal promoter and a common promoter, wherein said minimal promoters is operably linked to said common promoter, in opposite orientation to said common promoter, and 5′ to said common promoter. Those artificial nucleic acid constructs, wherein said bidirectional promoter further comprises at least one gene operably linked to said minimal promoter and said common promoter are preferred.

Cytochrome P450 Enzymes And Related Compounds And Methods, Michael Barrett

Plant and Soil Sciences Faculty Patents

The present invention provides, inter alia, nucleic acids which encode P450s in corn that, when expressed in the presence of a reductase, metabolize compounds exemplary of several distinct classes of insecticides and herbicides. The invention also includes amino acids encoded by the nucleic acids, as well as vectors, cells and eukaryotes comprising the nucleic or amino acid compounds. Also included are methods using the materials provided.

Forage News [2002-04], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• 2002 Hay Winners Recognized
• Spring Grazing School is April 17-18 in Stanford
• Distance Cattle Travel to Water Affects Pasture Utilization Rate
• Disk vs. Sicklebar Mowers
• Importance of Alfalfa
• Alfalfa Rooting Depth
• Upcoming Events

Effectiveness Of Current Therapy Of Bacterial Vaginosis, Petya M Andreeva, Hatim A. Omar

Pediatrics Faculty Publications

The study was conducted in order to evaluate effectiveness of the treatment of bacterial vaginosis (BV) with different therapeutic regimes according to recommendations of the World Health Organization (WHO). During a one-year period (February 2000-February 2001) the Sexually Transmitted Diseases (STD) Center was visited by 482 women aged 14-51. The diagnosis of BV was established by standard methods: Amsel's clinical criteria and Gram stain of vaginal discharge. The first-line treatment was oral Metronidazole 2 g single dose. Second line was Metronidazole 500 mg twice daily orally for 7 days or oral Clindamycin 300 mg twice daily for seven days. BV …

Method For Storing And Retrieving Sequential Information, Philip W. Landfield, Olivier Thibault

Pharmacology and Nutritional Sciences Faculty Patents

A method and design is provided for distributing and storing sets of temporally ordered information in a systematic and sequential fashion. This method is based on a model of how the brain functions in the distribution and storage of temporally ordered memories, but it can also be applied to the design of new biological, electronic or optical devices. These devices may be used in the testing and development of new therapeutic drugs, in the detection of toxic agents or impaired performance, or in the development of new industrial and consumer devices in which the orderly storage of sequential information is …

Methods And Compositions For The Treatment Of Psoriasis, Kenneth A. Foon, Malaya Chatterjee

Microbiology, Immunology and Molecular Genetics Faculty Patents

This invention provides methods of treating psoriasis which entail eliciting an immune response in an individual against an antigen aberrantly expressed in psoriatic tissue, such as a ganglioside, in an individual. The anti-ganglioside immune response is elicited by administration of an antigen such as a ganglioside, an anti-idiotype moiety for a ganglioside, or a polynucleotide encoding an anti-idiotype moiety. Also described is a strategy for developing additional compositions for psoriasis. The compositions elicit an immunological response against a target antigen present on psoriatic tissue, which in turn can be detected using antibody affinity-purified from the serum of the treated subject. …

Genes Encoding Several Poly (Adp-Ribose) Glycohydrolase (Parg) Enzymes, The Proteins And Fragments Thereof, And Antibodies Immunoreactive Therewith, Myron Jacobson, Elaine L. Jacobson, Jean-Christoph Amé, Winston Lin

Graduate Center for Nutritional Sciences Faculty Patents

The isolation and characterization of cDNAs encoding poly(ADP-ribose) glycohydrolase (PARG) enzymes and the amino acid sequences of PARGs from several species are described. PARG is involved in the cellular response to DNA damage and its proper function is associated with the body's response to neoplastic disorder inducing agents and oxidative stress. Expression vectors containing the cDNAs and cells transformed with the vectors are described. Probes and primers that hybridize with the cDNAs are described. Expression of the cDNA in E. coli results in an enzymatically active protein of about 111 kDa and an active fragment of about 59 kDa. Methods …

Materials And Methods Useful To Affect Growth And Development Of Lepidoptera Larvae, Douglas Lee Dahlman, Bruce Allen Webb, Indu B. Maiti

Kentucky Tobacco Research and Development Center Faculty Patents

The present invention provides materials and methods related to causing insect resistance in plants. Specifically, the present invention provides nucleic acid compounds which encode a teratocyte secreted protein (TSP) which is capable of causing inhibition of growth and developmental arrest in Lepidoptera larvae. Cells and plants (including plant parts, seeds, embryos, etc.) comprising the nucleic acid compounds are also within the scope of the present invention. The present invention also provides amino acid compounds encoded by the nucleic acid compounds of the present invention, as well as methods to induce larval developmental arrest, methods to produce the nucleic acid and …

Adolescent Substance Use And Abuse, Finn Green

Kaleidoscope

No abstract provided.

Endophyte Ergot Alkaloid Synthetic Compounds, Compounds Which Encode Therefor And Related Methods, Christopher L. Schardl, Jinhung Wang

Plant Pathology Faculty Patents

The present invention provides, inter alia, dmaW nucleic acid sequences and the proteins for which they encode. Also provided are methods for the utilization of knockout mutants of the sequences which are useful for engineering ergot alkaloid-deficient fungal symbionts (endophytes) of plants. Other methods and materials related to these sequences are also provided.

Summer Research And Creativity Grants, Uk Office Of Undergraduate Research

Kaleidoscope

No abstract provided.

The Hiv Protease Inhibitor Saquinavir Impairs Lipid Metabolism And Glucose Transport In Cultured Adipocytes, S. Ranganathan, Philip A. Kern

Clinical and Translational Science Faculty Publications

Treatment of HIV infection using protease inhibitors is frequently associated with lipodystrophy and impaired lipid and glucose metabolism. We examined the effect of saquinavir, one of the protease inhibitors, on lipid metabolism and glucose transport in cultured adipocytes. Saquinavir inhibited lipoprotein lipase (LPL) activity in 3T3-F442A and 3T3-L1 adipocytes. The inhibition of LPL was 81% at a concentration of 20 μg/ml. Another closely related drug, indinavir, had a small inhibitory effect. Saquinavir also inhibited the biosynthesis of lipids from [¹⁴C]-acetate. Saquinavir increased the lipolysis. Saquinavir had no significant effect on the cellular protein synthesis or protein content. Saquinavir …

The Federal Government's Role In Protecting Public Health, Ernie Fletcher

Kentucky Law Journal

No abstract provided.

Kentucky Water Resources Research Institute Annual Technical Report Fy 2001, Kentucky Water Resources Research Institute, University Of Kentucky

KWRRI Annual Technical Reports (USGS’s 104b Grant Program)

The FY 2001 Annual Technical Report for Kentucky consolidates reporting requirements of the Section 104(b) base grant award in a single technical report that includes: 1) a synopsis of each research project supported during the period, 2) a list of related reports, 3) a description of information transfer activities, 4) a summary of student support during the reporting period, and 5) notable achievements and awards during the year.