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C And N Cycling Dynamics And Microbial Community Composition In High Tunnel Production Systems, Kavita Lakshmi Mizin

Theses and Dissertations--Plant and Soil Sciences

Across the United States, high tunnels are used as a means of season extension and control over climatic variability in fruit and vegetable production. However, high tunnel systems are an intensive form of agricultural production which may compromise soil and crop quality over time. This study examined the effects of the varying environmental conditions, created in two types of tunnel system, movable and stationary, on the flow of nitrogen from amendment to plant and impact on crop and soil quality parameters. The way nutrients cycled through these systems was different and the nutrient treatments had no consistent effects on soil …

Evaluating The Effect Maturity On The Intake And Digestibility Of Switchgrass Hay Consumed By Beef Steers, David H. Davis

Theses and Dissertations--Plant and Soil Sciences

There has been increased interest in utilizing switchgrass (Panicum virgatum) as biomass. There are several challenges to developing this industry, and these have led to the potential use of switchgrass as hay for feeding beef cattle in Kentucky. The effect of increasing maturity on crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF) and nutritive values of switchgrass hay has been well documented, but few in vivo intake and digestibility trials have been conducted to assess this effect on animal performance when feeding beef cattle. Two in vivo intake and digestibility trials were conducted in 2011 …

Bacteria In Bioethanol Fermentations, Qing Li

Theses and Dissertations--Plant and Soil Sciences

To gain a better understanding of contaminating bacteria in bioethanol industry, we profiled the bacterial community structure in corn-based bioethanol fermentations and evaluated its correlation to environmental variables. Twenty-three batches of corn-mash sample were collected from six bioethanol facilities. The V4 region of the collective bacterial 16S rRNA genes was analyzed by Illumina Miseq sequencing to investigate the bacterial community structure. Non-metric multidimensional scaling (NMDS) ordination plots were constructed to visualize bacterial community structure groupings among different samples, as well as the effects of multiple environmental variables on community structure variation. Our results suggest that bacterial community structure is facility-specific, …

Iron And Iron Oxide Functionalized Membranes With Applications To Selected Chloro-Organic And Metal Removal From Water, Minghui Gui

Theses and Dissertations--Chemical and Materials Engineering

The development of functionalized membranes with tunable pores and catalytic properties provides us an opportunity to manipulate the membrane pore structure, selectivity and reactivity. By introducing the functional groups into membrane pores, dissolved metal ions and reactive particles can be effectively immobilized within the polymer matrix for toxic chloro-organic and heavy metal remediation in water.

A polyelectrolyte functionalized membrane platform with tunable pore size and ion exchange capacity has been developed for iron/iron oxide nano-catalyst synthesis and chlorinated organic compound (trichloroethylene, TCE and polychlorinated biphenyls, PCBs) degradation. Highly robust polyvinylidene fluoride (PVDF) microfiltration membranes are used as the support with …

Addressing Public Health Risks Of Persistent Pollutants Through Nutritional Modulation And Biomimetic Nanocomposite Remediation Platforms, Bradley J. Newsome

Theses and Dissertations--Chemistry

Due to their relative chemical stability and ubiquity in the environment, chlorinated organic contaminants such as polychlorinated biphenyls (PCBs) pose significant health risks and enduring remediation challenges. Engineered nanoparticles (NPs) provide a novel platform for sensing/remediation of these toxicants, in addition to the growing use of NPs in many industrial and biomedical applications, but there remains concern for their potential long-term health effects. Research highlighted herein also represents a transdisciplinary approach to address human health challenges associated with exposure to PCBs and NPs. The objectives of this dissertation research are two-fold, 1) to develop effective methods for capture/sensing and remediation …

The Effects Of Alternate-Line Shading On Visual Search In Grid-Based Graphic Designs, Michael P. Lee

Theses and Dissertations--Psychology

Objective: The goal of this research was to determine whether alternate-line shading (zebra-striping) of grid-based displays affects the strategy (i.e., “visual flow”) and efficiency of serial search. Background: Grids, matrices, and tables are commonly used to organize information. A number of design techniques and psychological principles are relevant to how viewers’ eyes can be guided through such visual works. One common technique for grids, “zebra-striping,” is intended to guide eyes through the design, or “create visual flow” by alternating shaded and unshaded rows or columns. Method: 13 participants completed a visual serial search task. The target was embedded in a …

Inflammatory Cytokine Gene Expression In Mesenteric Adipose Tissue During Acute Experimental Colitis, William Conan Mustain, Marlene E. Starr, Joseph Daniel Valentino, Donald A. Cohen, Daiki Okamura, Chi Wang, B. Mark Evers, Hiroshi Saito

Markey Cancer Center Faculty Publications

BACKGROUND: Production of inflammatory cytokines by mesenteric adipose tissue (MAT) has been implicated in the pathogenesis of inflammatory bowel disease (IBD). Animal models of colitis have demonstrated inflammatory changes within MAT, but it is unclear if these changes occur in isolation or as part of a systemic adipose tissue response. It is also unknown what cell types are responsible for cytokine production within MAT. The present study was designed to determine whether cytokine production by MAT during experimental colitis is depot-specific, and also to identify the source of cytokine production within MAT.

METHODS: Experimental colitis was induced in 6-month-old C57BL/6 …

The Supplemental Nutrition Assistance Program And Food Insecurity, Christian Gregory, Matthew P. Rabbitt, David C. Ribar

University of Kentucky Center for Poverty Research Discussion Paper Series

This chapter reviews recent theory and empirical evidence regarding the effect of SNAP on food insecurity and replicates the modelling strategies used in the empirical literature. The authors find that recent evidence suggesting an ameliorative effect of SNAP on food insecurity may not be robust to specification choice or data. Most specifications mirror the existing literature in finding a positive association of food insecurity with SNAP participation. Two-stage least squares and control function methods do show that SNAP reduces food insecurity, but effects are not consistent across sub-populations and are not always statistically significant.

Forage News [2013-12], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Thank You Kentucky Farmers
• Forages at KCA
• Forage Variety Reports Now Available
• Southern Forages Translated Into Spanish
• Small Ruminant Grazing Conference to be held in Lexington on February 1, 2014
• AFGC Annual Conference - Memphis, TN
• 34th Kentucky Alfalfa Conference
• Updated Analysis on Fungicides and Alfalfa

Snap And The School Meal Programs, Judith Bartfeld

University of Kentucky Center for Poverty Research Discussion Paper Series

The Great Recession and its immediate aftermath have brought increasing attention both to food insecurity among children and to the associated food safety net. After a decade of largely stable food insecurity rates, the share of children living in food insecure households jumped by one-third between 2007 and 2008, and has remained stubbornly high since then. As of 2012, 21.6% of all children lived in food insecure households (Coleman-Jensen et al. 2013). The scope and reach of the food safety net for children has likewise grown – a response to rising need, efforts to reduce administrative and logistical barriers to …

Early Flowering Mutant Chia And Uses Thereof, David Hildebrand, Watchareewan Jamboonsri, Timothy Phillips

Plant and Soil Sciences Faculty Patents

The invention relates to early flowering Chia (Salvia hispanica L.) strains that are suitable for culturing in a temperate area for seed production. Mutations are introduced to wild type chia seeds. Desired mutant progeny having normal appearance and an altered flora organ development is subsequently identified.

New Health Delivery Networks: Merging Public Health And Health Care Systems, Glen P. Mays

Health Management and Policy Presentations

Incomplete coordination between medical care and public health delivery systems can result in significant missed opportunities for improving population health and constraining overall resource use. This lecture uses insight from the field of public health services and systems research (PHSSR) to examine: (1) why medical care and public health systems often fail to connect; (2) what are the potential health and economic consequences of these failures; and (3) what are the opportunities for connecting medical care and public health delivery to improve population health.

Autonomic Nervous System Function Following Prenatal Opiate Exposure, Matthew Todd Hambleton, Eric W. Reynolds, Thitinart Sithisarn, Stuart J. Traxel, Abhijit R. Patwardhan, Timothy N. Crawford, Marta S. Mendiondo, Henrietta S. Bada

Pediatrics Faculty Publications

In utero exposure to opiates may affect autonomic functioning of the fetus and newborn. We investigated heart rate variability (HRV) as a measure of autonomic stability in prenatal opiate-exposed neonates (n = 14) and in control term infants (n = 10). Electrocardiographic data during both non-nutritive and nutritive sucking were evaluated for RR intervals, heart rate (HR), standard deviation of the consecutive RR intervals (SDRR), standard deviation of the differences of consecutive RR intervals (SDDRR), and the power spectral densities in low and high frequency bands. In controls, mean HR increased significantly, 143-161 per min (p = …

Pastures For Horses, Robert Coleman

Kentucky Grazing Conference

The horse evolved as a grazing animal. In order to consume enough forage to meet the horses’ nutritional needs a significant amount of time would be spent each day grazing. The pattern of selecting forage-moving to the next area starting to graze again can easily translate into 14 to 16 hours of grazing activity each day. The level of grazing activity will also be affected by the available forage and the daily access to pasture.

Pastures For Goats And Sheep, Greg Brann

Kentucky Grazing Conference

Pasture for sheep is not that different than that for cattle but pasture for goats is very different. Sheep are grazers like cattle whereas goats are browsers like deer. Even though grass and clover are not a goats preference if all of the forage present is grass and clover goats will eat it. Due to goats and sheep being more prey animals than cattle they don’t like to wade off into tall vegetation, also it takes more energy for them to move through tall growth. Many plants that we traditionally consider weeds become desirable forbs when goats or sheep are …

(How I Think About) Kentucky's Pastures: One Of Kentucky's Untapped Resources, Jimmy C. Henning

Kentucky Grazing Conference

One of the privileges of old(er) age is the prerogative to take liberties with assigned tasks. At the risk of confusing producers and offending more scientifically-current forage scientists, the topic of pasture as an untapped resource presented an opportunity to address task from an unexpected angle. Certainly, Kentucky’s pastures are a resource with untapped potential. But the idea that their value as a resource depends on how we think about them is one worth pursuing.

Sorghum Mutant Rg Displays Antithetic Leaf Shoot Lignin Accumulation Resulting In Improved Stem Saccharification Properties, Carloalberto Petti, Anne E. Harman-Ware, Mizuki Tateno, Rekha Kushwaha, Andrew Shearer, A. Bruce Downie, Mark Crocker, Seth Debolt

Horticulture Faculty Publications

BACKGROUND: Improving saccharification efficiency in bioenergy crop species remains an important challenge. Here, we report the characterization of a Sorghum (Sorghum bicolor L.) mutant, named REDforGREEN (RG), as a bioenergy feedstock.

RESULTS: It was found that RG displayed increased accumulation of lignin in leaves and depletion in the stems, antithetic to the trend observed in wild type. Consistent with these measurements, the RG leaf tissue displayed reduced saccharification efficiency whereas the stem saccharification efficiency increased relative to wild type. Reduced lignin was linked to improved saccharification in RG stems, but a chemical shift to greater S:G ratios in RG stem …

Forage News [2013-10], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• 14th Kentucky Grazing Conference - October 10
• Kentuckians attend IGC
• Clayton Geralds elected to National Hay Association Board
• NAFA's 2013 Alfalfa Intensive Training Seminar (AITS) - Register Today!
• Prussic Acid Poisoning and Frost
• AFGC to meet in Memphis
• New Research Leader, Forage-Animal Production Research Unit, Lexington, Kentucky Announced
• NRCS Technical Service Provider Program
• Historic Agricultural Data Now Online
• Mobile App Translates Per-Bale to Per-Ton Price

Clinical Inertia In Type 2 Diabetes: A Retrospective Analysis Of Pharmacist-Managed Diabetes Care Vs. Usual Medical Care, Felix K. Yam, Aimee G. Adams, Holly Divine, Douglas Steinke, Mikael D. Jones

Pharmacy Practice and Science Faculty Publications

Background: Evidence suggests that patients with type 2 diabetes (T2DM) suffer from a high rate of “clinical inertia” or “recognition of the problem but failure to act.”

Objective: The aim of this study is to quantify the rate of clinical inertia between two models of care: Pharmacist-Managed Diabetes Clinic (PMDC) vs. Usual Medical Care (UMC).

Methods: Patients in a university based medical clinic with type 2 diabetes (T2DM) were analyzed in this retrospective cohort study. Patients were exposed to either PMDC or UMC. The difference in days to intervention in response to suboptimal laboratory values and time …

The Effect Of Snap On Poverty, Laura Tiehen, Dean Jolliffe, Timothy Smeeding

University of Kentucky Center for Poverty Research Discussion Paper Series

On signing the Food Stamp Act of 1964, President Johnson noted that “as a permanent program, the food stamp plan will be one of our most valuable weapons for the war on poverty” (Johnson, 1964). From a humble beginning of 2.9 million recipients per month and $228 million in benefits in 1969 (earliest national figures), the program has grown to serve 47 million persons with benefits of over $74.6 Billion in 2012 (USDA, 2013a). In the late 60’s and early 70’s, when the average monthly benefit was under $20 per month per month, we did not record the effects of …

The Ral Exchange Factor Rgl2 Promotes Cardiomyocyte Survival And Inhibits Cardiac Fibrosis, Rebecca L. Scotland, Leah Allen, Leah J. Hennings, Ginell R. Post, Steven R. Post

Pharmacology and Nutritional Sciences Faculty Publications

Cardiomyocytes compensate to acute cardiac stress by increasing in size and contractile function. However, prolonged stress leads to a decompensated response characterized by cardiomyocyte death, tissue fibrosis and loss of cardiac function. Identifying approaches to inhibit this transition to a decompensated response may reveal important targets for treating heart failure. The Ral guanine nucleotide disassociation (RalGDS) proteins are Ras-interacting proteins that are upregulated by hypertrophic stimuli. The Ral guanine nucleotide dissociation stimulator-like 2 (Rgl2) is a member of the RalGDS family that modulates expression of hypertrophic genes in cardiomyocytes. However, the pathophysiologic consequence of increased Rgl2 expression in cardiomyoctyes remains …

2012 Kentucky Equine Survey, College Of Agriculture, Food And Environment, University Of Kentucky, Kentucky Agricultural Development Fund, Kentucky Horse Council, Ag Equine Programs, University Of Kentucky

Kentucky Equine Survey

The last thorough equine inventory in Kentucky was undertaken in 1977, leaving the state’s equine industry with 35 years of changes, growth and con­traction that were neither measured nor analyzed.

In response to these needs, in 2011, the University of Kentucky and the Kentucky Horse Council, with support by industry organizations spearheaded by the Kentucky Quarter Horse Association, the Kentucky Thoroughbred Farm Managers’ Club and Kentucky Thoroughbred Association/Kentucky Thoroughbred Owners and Breeders, and initial support from the University of Louisville, succeeded in securing fund­ing for a large-scale, comprehensive equine survey upon which future industry plans could be built. This was …

Why Are So Many Americans On Food Stamps? The Role Of The Economy, Policy, And Demographics, James P. Ziliak

University of Kentucky Center for Poverty Research Discussion Paper Series

1 in 7 Americans received assistance from SNAP in FY2012, which is a rate 141 percent higher than in FY2000, but only 59 percent higher than in FY1980. In this chapter I describe the socioeconomic and policy climate in recent decades that had bearing on SNAP participation, along with a formal empirical analysis of those determinants and detailed simulations of the relative contributions of the economy, policy, and demographics to changes in SNAP participation over time. The results suggest that SNAP is operating effectively as an automatic fiscal stabilizer—nearly 50 percent of the increase in participation from 2007-2011 is due …

A Diffusible Mobile Dental Services Model For Prevention-Focused Outreach For Underserved Children In Rural Communities, Nikki Stone, Frances J. Feltner, Willie Bates

Center of Excellence in Rural Health Presentations

Background: In 2006 a mobile dental outreach program began operating full-time at the UK North Fork Valley Community Health Center in Hazard, Kentucky, a federally-qualified health center administered by the University of Kentucky and located at the UK Center of Excellence in Rural Health. This program continues to provide preventive dental care to children at forty different elementary schools and Head Start centers in a four county area of southeastern Kentucky located in the heart of rural Appalachia. The counties are Leslie, Knott, Letcher and Perry (LKLP), which are part of the Kentucky River Development District and encompass the LKLP …

The Influence Of Parental Aspirations, Attitudes, And Engagement On Children's Very Low Food Security, Elizabeth T. Powers

University of Kentucky Center for Poverty Research Discussion Paper Series

Survey of Income and Program Participation data are used to investigate the relationship between parenting and children’s very low food security. Parenting is characterized along five domains (emotional outlook, support, education desires, activities with the child excluding meals, and television viewing rules). Food security definitions are obtained from questions in a special SIPP module that are based on the USDA’s core food security module. Graphical evidence indicates that parenting patterns differ distinctly for households experiencing various levels of food insecurity. Descriptive regression evidence suggests that some of the parenting attributes are significantly associated with children’s food insecurity, even controlling for …

Risk And Protective Factors Associated With Prevalence Of Vlfs In Children Among Children Of Foreign-Born Mothers, John Cook

University of Kentucky Center for Poverty Research Discussion Paper Series

This research examined VLFS in children among households with foreign-born (FB) mothers compared to US-born mothers through three research questions: Is mother’s foreign-born status (FBS) associated with VLFS in children, and can association be explained by mothers’ socio-demographic characteristics? Are FB mothers more or less likely to receive nutrition or non-nutrition assistance benefits, or work for pay than US-born mothers? Do mothers’ FBS, or protective/risk factors associated with FBS, modify associations of negative economic shocks and hardships with VLFS in children? Data are on approximately 44,000 mother-child (ages<48 Mos.) dyads collected from household surveys administered under a "sentinel surveillance" system over 1998-2012 at teaching hospitals and clinics in seven US cities. Bivariate and multivariate logistic regression models tested study hypotheses. Mothers' FBS is strongly positively associated with VLFS in children after controlling for available risk and protective factors. FB mothers are less likely to receive SNAP and non-nutrition assistance (TANF, LIHEAP or housing subsidies), but more likely to receive WIC and to be employed than US-born mothers. FB mothers are no more likely to report negative reasons for not receiving SNAP or TANF, or losing jobs or decreasing work hours than US-born, and reported "immigration concerns" rarely. No need/chose not to participate are most frequently reported reasons for not receiving SNAP and TANF; pregnancy/maternity leave and "market conditions" for lost jobs and decreased work hours. Economic shocks and hardships are positively associated with VLFS in children, but Mothers' FBS does not interact with shocks and hardships to modify those associations.

Food Insecurity During Childhood: Understanding Persistence And Change Using Linked Current Population Survey Data, Sheela Kennedy, Catherine A. Fitch, John Robert Warren, Julia A. Rivera Drew

University of Kentucky Center for Poverty Research Discussion Paper Series

Our paper examines the prevalence and determinants of children’s transitions into and out of food insecurity since 2001. We use longitudinally linked data from the Food Security Supplements to the Current Population Surveys to estimate one-year transition probabilities of entry and exit from food insecurity. Our results indicate that child hunger is typically short-lived, but children experiencing very low food security frequently experience multiple consecutive years of food insecurity. We demonstrate large demographic and socioeconomic differences in rates of entry into very low food security and persistence in children's food insecurity. Income and employment shocks are important predictors of child …

Use Of Alternative Financial Services And Childhood Food Insecurity, Katie Fitzpatrick

University of Kentucky Center for Poverty Research Discussion Paper Series

Low- and moderate-income (LMI) households with children often face considerable difficulties in ensuring enough financial resources for an adequate diet. This project investigates the use of financial services and other financial decisions parents make that may affect the risk of very low food security and food insecurity of children. With households in both the December 2008 Current Population Survey (CPS) Food Security Supplement and the January 2009 CPS Unbanked and Underbanked Supplement, the project studies the relationship between bank account ownership, use of alternative financial service (AFS) providers, the organization of household finances, and the food security of children. Both …

Fecal Transplantation Does Not Transfer Either Susceptibility Or Resistance To Food Borne Listeriosis In C57bl/6 And Balb/C/By Mice, Tanya Myers-Morales, Kate M. Bussell, Sarah E. F. D'Orazio

Microbiology, Immunology, and Molecular Genetics Faculty Publications

The composition of the intestinal microbiota has wide reaching effects on the health of an individual, including the development of protective innate immune responses. In this report, a fecal transplantation approach was used to determine whether resistance to food borne listeriosis was dependent on the murine gut microbiota. Transplantation of BALB/c/By feces did not increase the susceptibility of C57BL/6 mice to Listeria monocytogenes infection. Likewise, transplantation of C57BL/6 fecal matter did not enhance the resistance of BALB/c/By mice. Thus, intestinal microbiota composition is not a key factor that confers either susceptibility or resistance to food borne listeriosis in mice.

Dose- And Time-Dependent Neuroprotective Effects Of Pycnogenol® Following Traumatic Brain Injury, Mubeen A. Ansari, Kelly N. Roberts, Stephen W. Scheff

Sanders-Brown Center on Aging Faculty Publications

After traumatic brain injury (TBI), both primary and secondary injury cascades are initiated, leading to neuronal death and cognitive dysfunction. We have previously shown that the combinational bioflavonoid, Pycnogenol® (PYC), alters some secondary injury cascades and protects synaptic proteins when administered immediately following trauma. The purpose of the present study was to explore further the beneficial effects of PYC and to test whether it can be used in a more clinically relevant fashion. Young adult male Sprague-Dawley rats were subjected to a unilateral moderate/severe cortical contusion. Subjects received a single intravenous (i.v.) injection of PYC (1, 5, or 10 mg/kg) …