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Adaptation Of Conventional Wheat Flour Mill To Refine Sorghum, Corn, And Cowpea, Michael Joseph, Sajid Alavi, Akinbode A. Adedeji, Lijia Zhu, Jeff Gwirtz, Shawn Thiele Jan 2024

Adaptation Of Conventional Wheat Flour Mill To Refine Sorghum, Corn, And Cowpea, Michael Joseph, Sajid Alavi, Akinbode A. Adedeji, Lijia Zhu, Jeff Gwirtz, Shawn Thiele

Biosystems and Agricultural Engineering Faculty Publications

This study evaluated the refinement of sorghum, corn, and cowpea grains using the processing steps and equipment originally designed for wheat milling that consists of a conventional gradual reduction system. The need to mill these grains resulted from a desire to produce alternative ingredients for developing new fortified blended extruded foods used for food aid programming. Milling of white sorghum grain resulted in a crude protein content of 7.4% (wb) for both whole and coarse-milled flour. The crude protein content in whole fine-milled sorghum was 6.8% (wb), which was significantly lower than that of whole coarse flour at 9.3% (wb). …


Design And Optimization Of High-Efficiency Coreless Pcb Stator Axial Flux Pm Machines With Minimal Eddy And Circulating Current Losses, Yaser Chulaee, Greg Heins, Ali Mohammadi, Mark Thiele, Dan M. Ionel, Ben Robinson Jan 2024

Design And Optimization Of High-Efficiency Coreless Pcb Stator Axial Flux Pm Machines With Minimal Eddy And Circulating Current Losses, Yaser Chulaee, Greg Heins, Ali Mohammadi, Mark Thiele, Dan M. Ionel, Ben Robinson

Electrical and Computer Engineering Graduate Research

This paper proposes a systematic multi-step design procedure for highly efficient printed circuit board (PCB) stator coreless axial flux permanent magnet (AFPM) machines with minimal eddy and circulating current losses. The process begins with initial sizing, providing specific coefficients based on experience with multiple design projects. It continues with the optimization of the machine envelope design using an evolutionary algorithm and computationally efficient 3D finite element analysis (FEA) models. The subsequent step focuses on the detailed design of a PCB stator, aiming to minimize eddy and circulating current losses. Several open circuit loss mitigation techniques are proposed based on analytical …


Design Optimization Of A Direct-Drive Wind Generator With A Reluctance Rotor And A Flux Intensifying Stator Using Different Pm Types, Ali Mohammadi, Oluwaseun A. Badewa, Yaser Chulaee, Donovin D. Lewis, Somasundaram Essakiappan, Madhav Manjrekar Jan 2024

Design Optimization Of A Direct-Drive Wind Generator With A Reluctance Rotor And A Flux Intensifying Stator Using Different Pm Types, Ali Mohammadi, Oluwaseun A. Badewa, Yaser Chulaee, Donovin D. Lewis, Somasundaram Essakiappan, Madhav Manjrekar

Electrical and Computer Engineering Graduate Research

This paper presents a large-scale multi-objective design optimization for a direct-drive wind turbine generator concept that is based upon an experimentally validated computational model for a small-scale prototype motor of the same type. By integrating an outer reluctance-type rotor and a segmented stator with toroidally wound single-coil modules containing spoke-type PMs, the design optimization aims to minimize losses, active mass, and torque ripple while adhering to a power factor constraint. The AC windings and PMs are positioned in the stator and this concept enhances flux concentration, enabling the use of more affordable high energy non-rare-earth (special type) magnets. The exterior …


Large-Scale Design Optimization Of An Axial-Flux Vernier Machine With Dual Stator And Spoke Pm Rotor For Ev In-Wheel Traction, Ali Mohammadi, Yaser Chulaee, Aaron M. Cramer, Ion G. Boldea, Dan M. Ionel Jan 2024

Large-Scale Design Optimization Of An Axial-Flux Vernier Machine With Dual Stator And Spoke Pm Rotor For Ev In-Wheel Traction, Ali Mohammadi, Yaser Chulaee, Aaron M. Cramer, Ion G. Boldea, Dan M. Ionel

Electrical and Computer Engineering Graduate Research

This paper presents the optimization study targeting a specific drive cycle for a MAGNUS-type axial-flux permanent magnet vernier machine (AFPMVM). The proposed MAGNUS machine has a novel design with a dual-stator configuration, where only one stator is wound, with a high-polarity spoke permanent magnet (PM) rotor. The machine topology has a 3D flux path, which necessitates the analysis of a large finite element (FE) model. However, due to the computational complexity and time required for such a large FE model, a new approach was developed. This approach involves a computationally efficient finite element analysis (CE-FEA) model combined with a single …


A Social History Of Opioids’ Crimedical Cycle, Jerel M. Ezell, Alex Rains, Babatunde Patrick Ajayi, Kyle Miller, Erin Augustine, Dawn Goddard-Eckrich, Elizabeth N. Kinnard, Michelle R. Lofwall Jan 2024

A Social History Of Opioids’ Crimedical Cycle, Jerel M. Ezell, Alex Rains, Babatunde Patrick Ajayi, Kyle Miller, Erin Augustine, Dawn Goddard-Eckrich, Elizabeth N. Kinnard, Michelle R. Lofwall

Behavioral Science Faculty Publications

Historically, public and political discourse around people who nonmedically use opioids has largely been rendered via a deficits-oriented lens, presenting this population as weak, disinterested, and deviant,and thus undesirable and ill-positioned for wellness and communal integration. These narratives, and subsequent policies used to engage people who use opioids, are intermittently formed and disrupted by geopolitical changes, as public and institutional attitudes, often driven by racial and class-related considerations, shift. As part of what we term the crimedical cycle, a population’s behaviors fluctuate between periods of medicalization and criminalization. Strengths-based theory, an offshoot of ‘positive psychology’ that helps contextualize the crimedical …


Astrocytic Mitochondrial Transfer To Brain Endothelial Cells And Pericytes In Vivo Increases With Aging, Gopal V. Velmurugan, Hemendra J. Vekaria, Samir P. Patel, Patrick G. Sullivan, W. Brad Hubbard Jan 2024

Astrocytic Mitochondrial Transfer To Brain Endothelial Cells And Pericytes In Vivo Increases With Aging, Gopal V. Velmurugan, Hemendra J. Vekaria, Samir P. Patel, Patrick G. Sullivan, W. Brad Hubbard

Neurology Faculty Publications

Intercellular mitochondrial transfer (IMT) is an intriguing biological phenomenon where mitochondria are transferred between different cells and notably, cell types. IMT is physiological, occurring in normal conditions, but also is utilized to deliver healthy mitochondria to cells in distress. Transferred mitochondria can be integrated to improve cellular metabolism, and mitochondrial function. Research on the mitochondrial transfer axis between astrocytes and brain capillaries in vivo is limited by the cellular heterogeneity of the neurovascular unit. To this end, we developed an inducible mouse model that expresses mitochondrial Dendra2 only in astrocytes and then isolated brain capillaries to remove all intact astrocytes. …


Using Digital Pathology To Analyze The Murine Cerebrovasculature, Dana M. Niedowicz, Jenna L. Gollihue, Erica M. Weekman, Panhavuth Phe, Donna M. Wilcock, Christopher M. Norris, Peter T. Nelson Jan 2024

Using Digital Pathology To Analyze The Murine Cerebrovasculature, Dana M. Niedowicz, Jenna L. Gollihue, Erica M. Weekman, Panhavuth Phe, Donna M. Wilcock, Christopher M. Norris, Peter T. Nelson

Neurology Faculty Publications

Research on the cerebrovasculature may provide insights into brain health and disease. Immunohistochemical staining is one way to visualize blood vessels, and digital pathology has the potential to revolutionize the measurement of blood vessel parameters. These tools provide opportunities for translational mouse model research. However, mouse brain tissue presents a formidable set of technical challenges, including potentially high background staining and crossreactivity of endogenous IgG. Formalin-fixed paraffin-embedded (FFPE) and fixed frozen sections, both of which are widely used, may require different methods. In this study, we optimized blood vessel staining in mouse brain tissue, testing both FFPE and frozen fixed …


Ruling In The Shadows: Analysis Of The Supreme Court’S Use Of The ‘Shadow Docket’ And Its Effects, Emilee Smart Jan 2024

Ruling In The Shadows: Analysis Of The Supreme Court’S Use Of The ‘Shadow Docket’ And Its Effects, Emilee Smart

Theses and Dissertations--Political Science

The recent increased use of the shadow docket has left the public and scholars with unanswered questions about how procedures influence outcomes and behavior. Many of these shadow docket cases have been petitioned to the justices as emergencies in very important policy areas such as immigration, abortion, elections, and transgender rights. I collect a large dataset of all outcomes of the Supreme Court's shadow docket from 2010-2022. I examine the language the justices use to justify their decisions made using alternate procedures. I find unique differences in the justifying behavior of the justices as well as significant differences in the …


Public Justice: Analyzing The Interactions Of Supreme Court Justices With The American Public, Abbie Wood Jan 2024

Public Justice: Analyzing The Interactions Of Supreme Court Justices With The American Public, Abbie Wood

Theses and Dissertations--Political Science

When and how do Supreme Court justices choose to interact with the public? What motivating factors spur a justice to make a public appearance? In the fields of political science, public law, and legal studies, research has overwhelmingly studied on-the-bench behavior. Scholars have analyzed judicial voting patterns, opinion writing, oral arguments, and more. Despite the prevalence and importance of non-decision making activities as well as the growing reporting by media outlets concerning justices' public appearances, there remains a lack of attention devoted to exploring how justices behave off-the-bench. In this dissertation, I seek to develop this neglected area within judicial …


Evaluating A High School Mrsa Prevention Program: A Case Study, Jamie Henning Jan 2024

Evaluating A High School Mrsa Prevention Program: A Case Study, Jamie Henning

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

MRSA poses a significant health risk to athletes nationwide. This case study examines the application of an online training module to address knowledge gaps regarding Methicillin-resistant Staphylococcus aureus (MRSA) among key decision-makers for high school athletes. It reviews the development and initial evaluation of a web-based training program designed to empower decision-makers with the knowledge to prevent MRSA infection and respond to suspected cases. Program evaluation recommended expanding the training module to wrestling staff with improved evaluation methods while continuing implementation and effectiveness assessment for the football staff. Despite initial data quality limitations, the online training module evaluation offered valuable …


A Case Study In Evaluation, Laura Nagle Jan 2024

A Case Study In Evaluation, Laura Nagle

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

The Lexington – Fayette County Health Department is proposing a comprehensive evaluation of programming focused on preventing MRSA infections among high school athletes within Fayette County Public Schools. This document describes the recommended evaluation process.


Team Up Against Overdose Deaths, Mary Elizabeth Pendergrass Jan 2024

Team Up Against Overdose Deaths, Mary Elizabeth Pendergrass

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

This grant proposal describes a community collaboration strategy for reducing overdose deaths. Utilizing a Community Advisory Board, the goal of the project is to increase awareness, knowledge, and usage of the Good Samaritan Law to obtain emergency medical care.


Changes In Primary Care Availability In Appalachia, Whitney Beckett Jan 2024

Changes In Primary Care Availability In Appalachia, Whitney Beckett

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

The Affordable Care Act (ACA) was a groundbreaking moment in the United States healthcare system. It affected many different aspects of the system, including access to primary care professionals. Primary care professionals provide a key role in American’s health, especially when it comes to preventative care. With one-third of Americans experiencing barriers to such access, a solution is desperately needed to increase said access. These barriers are especially profound in low-income, underserved areas such as the Appalachian regions of Georgia, Kentucky, Tennessee, and West Virginia. The ACA attempted to increase access via Medicaid expansion; however, not all States expanded their …


Novel Cannabinoid Use Among Young Adults In Lexington, Kentucky, Victoria A. Hamilton Jan 2024

Novel Cannabinoid Use Among Young Adults In Lexington, Kentucky, Victoria A. Hamilton

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

Purpose: The purpose of this study was to implement a survey to estimate the prevalence of novel cannabinoid use among young adults in Lexington, Kentucky, and to examine modes of use and motivations for novel cannabinoid use. The primary hypothesis of this study was that many respondents would indicate medical reasons for using novel cannabinoids.

Methods: A cross-sectional primary data analysis of 101 young adults in Lexington, Kentucky to determine prevalence of lifetime novel cannabinoid use, past 30-day novel cannabinoid use, mode of use, motivation for use, and related demographic characteristics. Univariate and bivariate analysis were produced using the PROC …


Mccovid Campaign – A Social Media Implementation To Decrease Vaccine Hesitancy In Rural Counties, Harper Ford Jan 2024

Mccovid Campaign – A Social Media Implementation To Decrease Vaccine Hesitancy In Rural Counties, Harper Ford

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

This capstone will explore the challenges of vaccine hesitancy, a continuously rising problem in the public health field. The focus is primarily on rural millennials in McCracken County, Kentucky. The study identifies key factors contributing to vaccine hesitancy including misinformation, trust issues, and the rapid development of the covid-19 vaccine. To address the above concerns the study proposes a targeted social media campaign using the health belief model to increase COVID-19 vaccine uptake through Facebook ads targeting millennials. The campaigns utilize the leverage of local insights and real-time data to enhance engagement and reduce hesitancy, focusing on adapting strategies based …


Improving Black Maternal Outcomes In Christian County, Ky: A Social Marketing Approach To Perinatal Provider Change, Ariel A. Arthur Jan 2024

Improving Black Maternal Outcomes In Christian County, Ky: A Social Marketing Approach To Perinatal Provider Change, Ariel A. Arthur

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

Compared to other states in the country, Kentucky has historically poor population health outcomes. While the statewide maternal mortality rate is 16.1 per 100,000 live births, this statistic belies the stark racial disparities that exist. In 2018, the pregnancy-related death rate for Black women was 40.2 per 100,000 live births compared to 13.1 for White women. This pattern is persistent even after controlling for socioeconomic status, prenatal care, and other protective factors. Research shows that institutional and interpersonal racism when receiving obstetric care contributes to poor childbirth and postpartum outcomes for Black women. Additionally, geography and rural status may compound …


Implementation Of A Postpartum Depression Program In A Rural Kentucky County, Abby Cecil Jan 2024

Implementation Of A Postpartum Depression Program In A Rural Kentucky County, Abby Cecil

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

This paper aims to describe the process of implementing a Postpartum Depression (PPD) prevention program in low-income women residing in rural communities. The paper describes the process of recruiting women in this population, where and how the program will be integrated into an existing clinic, and how the program will be sustained. PPD is a significant global public health problem that can be prevented and treated. The ROSE program (Reach Out, Stay Strong, Essentials for new mothers) is a series of classes that provide informational and social support to prevent PPD. This evidence-based program has been previously successful in reducing …


Patient Access To Glp-1 Ra’S: A Medicare Part D Policy Analysis, Celine Hummer Jan 2024

Patient Access To Glp-1 Ra’S: A Medicare Part D Policy Analysis, Celine Hummer

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

This policy analysis examines the evolving landscape of GLP-1 receptor agonists (GLP-1 RAs) and their implications for Medicare Part D coverage. Initially developed for managing type 2 diabetes, GLP-1 RAs have rapidly gained popularity due to their efficacy in treating obesity and reducing cardiovascular events, leading to broader therapeutic applications. However, access to these medications under Medicare Part D is limited by high costs and coverage restrictions, particularly when used for obesity without diabetes. This analysis evaluates current Medicare Part D policies, including specialty drug tier placement, formulary restrictions, and the potential impact of recent legislative initiatives such as the …


How Important Is Parental Involvement And Engagement In Preschool, Emilee M. Dixon Jan 2024

How Important Is Parental Involvement And Engagement In Preschool, Emilee M. Dixon

Theses and Dissertations--Early Childhood, Special Education, and Counselor Education

The purpose of this study was to evaluate preschool classrooms’ parental engagement throughout the school year. Specifically, the study aims to assess if parents felt they received information to support their child's development, if the school offered engagement opportunities, and if the parents believed that engagement impacted their child. Research studies have shown or indicated that parental engagement is a key component to a child’s success. This study highlights the importance of parental involvement and engagement for children in preschool. The study involves reviewing a survey to gain a better understanding of when, how, and why parental involvement and engagement …


Comparison Of Video Modeling And In-Vivo Language Instruction In Preschool, Jessah Marlee Hughes Jan 2024

Comparison Of Video Modeling And In-Vivo Language Instruction In Preschool, Jessah Marlee Hughes

Theses and Dissertations--Early Childhood, Special Education, and Counselor Education

Early communication and language development in young children is critically important and those identified as having a language delay, would benefit from intervention. There is a rise in screen time exposure in young children, with some watching variations of popular evidence-based practices (time delay, video modeling, etc.) Most notably, Ms. Rachel is an early childhood learning YouTube sensation. This study seeks to compare the effectiveness and efficiency of video modeling and time delay in the differential use of early sentences during play-based activities.


More Than Censorship: The Harm Of Libricide, James M. Donovan Jan 2024

More Than Censorship: The Harm Of Libricide, James M. Donovan

Law Faculty Scholarly Articles

Libricide, although often deemed an extreme instance of censorship, is altogether different. Censorship involves the suppression of particular books due to alleged inappropriate content; libricide refers to the intentional destruction of entire libraries. Understanding the differing motives recognizes that the library is more than the books it contains, and is instead an institution rooted in its history of selection and use by the local community. Over time, the library reflects the users’ identity, a reminder that any aggressor would wish to eliminate when the goal is pacification by erasure of a population’s memory and history. Prerequisites for an act of …


Tools Do Not Create: Human Authorship In The Use Of Generative Artificial Intelligence, Michael D. Murray Jan 2024

Tools Do Not Create: Human Authorship In The Use Of Generative Artificial Intelligence, Michael D. Murray

Law Faculty Scholarly Articles

Artistic tools, from brushes to complex algorithms, don’t create art; human artists do. The advent of generative AI tools like Midjourney, DALL-E, and Stable Diffusion has blurred this understanding, causing observers to believe these tools are the authors of the artworks they produce, even so far as to imagine that the artworks are “created” by the AI in the copyright sense of the word. Not so.

The U.S. Copyright Office recently issued guidance on the copyrightability of works produced using generative AI tools. The Office has accepted the narrative that AI tools perform the steps of authorship, conceiving of the …


Media + Vigilante Violence: The Formula For American Atrocity, Tiffany D. Atkins Jan 2024

Media + Vigilante Violence: The Formula For American Atrocity, Tiffany D. Atkins

Law Faculty Scholarly Articles

This Article provides an overview of the international and domestic responses to media-fueled violence and demonstrates how both legal systems have failed to provide meaningful protections for Black Americans from vigilante violence and atrocity. Part I explores the history of media as a propaganda tool against underrepresented groups, detailing how it has promoted atrocity domestically and internationally. There are parallels between the historical and contemporary use of media as a method to fuel atrocity against minority groups; illustrating these examples indicates the continued risk of violence and atrocity faced by Black Americans due to these models of persuasive communication. Part …


Deepfakes And Dog Toys: First Amendment Defenses Under The Rogers Test After Jack Daniel’S V. Vip Products, Michael D. Murray Jan 2024

Deepfakes And Dog Toys: First Amendment Defenses Under The Rogers Test After Jack Daniel’S V. Vip Products, Michael D. Murray

Law Faculty Scholarly Articles

Given that deepfakes are almost always associated with expression of some kind, any attempt to litigate or regulate deepfakes will require the consideration of the First Amendment. Deepfakes implicate so many areas of law and society that a discussion of the interface of First Amendment free speech rights and deepfakes writ large would require a treatise volume with several chapters. This article is more modest: It will examine the past, present, and future use of the Rogers test in an evaluation of the application of First Amendment protections in trademark infringement cases and in legal actions traditionally associated with the …


Program Evaluation Case Study: Infectious Disease Reporting Training Program Evaluation, Emily Sekkath Veedu Jan 2024

Program Evaluation Case Study: Infectious Disease Reporting Training Program Evaluation, Emily Sekkath Veedu

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

The Lexington-Fayette County Health Department (LFCHD) has developed an educational program aimed at Fayette County Public Schools (FCPS) health and athletics staff members to ensure timely reporting and action in the event of an infectious disease outbreak (4). The program was implemented after a methicillin-resistant staphylococcus aureus (MRSA) outbreak occurred in a Kentucky community, with a high school football team presenting several of the cases (4). It was noted by a school nurse that many people attending an informational session lacked knowledge regarding infection control, suggesting a need for intervention in this community. I have evaluated this program in the …


Recent Trends In Stimulant Prescribing In Kentucky, 2021-2023, Jordan Shannon Jan 2024

Recent Trends In Stimulant Prescribing In Kentucky, 2021-2023, Jordan Shannon

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

Abstract

Introduction

Prescription stimulants have a long history of clinical use dating as far back as the 1930s. In the United States stimulant prescribing rates have steadily climbed over the last 3 decades. The primary objective is to describe recent (2021-2023) prescribing trends of stimulant medications in the Commonwealth of Kentucky

Methods

Prescription data collected from Kentucky’s prescription drug monitoring (PDMP) database known as the Kentucky All Schedule Prescription Electronic Reporting System (KASPER). Annual and quarterly trends in stimulant prescribing between 2021-2023 were calculated, examined by age, sex, and geographic location.

Results

Stimulant prescribing increased by ~15% where the majority …


Evaluating The Effect Of Sleep Hygiene Education On Sleep Quality Among First-Year College Students, Caroline Lowe Cunningham Jan 2024

Evaluating The Effect Of Sleep Hygiene Education On Sleep Quality Among First-Year College Students, Caroline Lowe Cunningham

DNP Projects

Abstract

Background: The American Academy of Sleep Medicine recommends college students should get seven to nine hours of sleep each night. Only one-third currently meet this recommendation. Poor sleep negatively affects academic performance, mood, and interpersonal relationships. Sleep hygiene is recommended to improve sleep quality and quantity.

Purpose: The purpose of this DNP project was to determine the effect of implementing a sleep hygiene educational intervention in classes intended for first-year college students at the University of Kentucky.

Methods: This quasi-experimental project utilized the Pittsburg Sleep Quality Index to assess participants’ sleep before an in-person sleep hygiene education and after …


A Change Of Character? Exploring The Possible Effects Of Non-Tenure Track Faculty At Liberal Arts Colleges, Robert Brown Jan 2024

A Change Of Character? Exploring The Possible Effects Of Non-Tenure Track Faculty At Liberal Arts Colleges, Robert Brown

Theses and Dissertations--Educational Policy Studies and Evaluation

Research on liberal arts colleges (LACs) has yet to consider possible effects of using Non-Tenure Track Faculty (NTTF) on the character of LACs. LACs are characterized by their strong academic ethos, collegial faculty-student relationships, all at a sanctuary-like setting. Tenure Track Faculty (TTF) and administrators at three LACs were interviewed in a case study format at an urban, suburban, and rural LAC to consider whether NTTF are affecting the character of such institutions. NTTF, especially when full-time faculty, support the academic ethos, foster strong student faculty relationships, and help maintain the sanctuary like setting. NTTF use by change agents is …


Working To Learn And Learning To Work: A Comparative Exploration Of Federal Work-Study Program Implementation Across Kentucky Colleges And Universities, Alexandria Wasson Jan 2024

Working To Learn And Learning To Work: A Comparative Exploration Of Federal Work-Study Program Implementation Across Kentucky Colleges And Universities, Alexandria Wasson

Theses and Dissertations--Educational Policy Studies and Evaluation

The Federal Work-Study (FWS) program aims to connect low-income college students with paid campus employment and career opportunities. Institutions also benefit, as they are supplied with a federally subsidized workforce to staff essential entry-level jobs. However, this well-intentioned financial aid program is affected by a lack of uniform guidance at a national level. Hard working financial aid professionals, in tandem with other college and university administrators, are empowered to make FWS decisions that afford some students opportunities for advancement and constrain other students from reaching their potential.

The purpose of this study is to learn about the design and implementation …


Developing An Immunomodulatory Strategy Using Biophysical Cues To Modulate Macrophage Phenotype For Fracture Healing And Bone Regeneration, Harshini Suresh Kumar Jan 2024

Developing An Immunomodulatory Strategy Using Biophysical Cues To Modulate Macrophage Phenotype For Fracture Healing And Bone Regeneration, Harshini Suresh Kumar

Theses and Dissertations--Biomedical Engineering

Chronic inflammation is a major cause of the pathogenesis of musculoskeletal diseases such as fragility, fracture, and nonunion. Studies have shown that modulating the immune phenotype of macrophages from proinflammatory to pro-healing can heal recalcitrant bone defects. Current therapeutic strategies predominantly apply biochemical cues, which often lack target specificity, and controlling their release kinetics in vivo is challenging spatially and temporally. We have developed a magnetic iron-oxide nanocomplexes (MNC)-based therapy for resolving chronic inflammation in the context of promoting fracture healing. Here, we show that MNC internalized macrophages, when coupled with an external magnetic field, can exert an intracellular magnetic …