Digital Commons Network^™

Assessing Factors Influencing Participation And Dissemination In Community-Based Public Health Coalitions: An Exploration Of Social Change, Chad Tyler Morris

University of Kentucky Doctoral Dissertations

The community-based public health coalition has proliferated in public health practice since the 1970‘s as a favored means of achieving community participation in public health promotion. There is concern, however, that many contemporary coalitions are not particularly inclusive, and that population health indicators fail to demonstrate significant improvement in health outcomes resultant from coalition practice. This dissertation research was designed to critically examine participation and dissemination of coalition-derived ideas through ethnographic study of five community-based participatory public health coalitions in the United States. The research answers calls from public health scholars to improve upon the coalition theory base and to …

Painting The Mountains: An Investigation Of Tourist Art In North America, Kristin Mary Agnes Helen Kant

University of Kentucky Doctoral Dissertations

This study examines the use of regional cultural icons, like hillbillies, nineteenth century pioneer caricatures, and rural/wilderness landscapes, in paintings from an Appalachian tourist center. These icons, produced by the public media’s portrayal of the Appalachian region over several generations, contribute to a sense of cultural difference associated with people of Appalachia. The research question driving this project is: would cultural distinctiveness exist if cultural stereotypes were not a part of the tourist center’s local economics, politics, and social life? Building on ideas from consumption studies, this project explores consumption practices of artists and tourists as they interact with icons …

Exploring Lesbian And Gay Experiences With Individuals, Systems, And Environments: Patterns Of Response To Heterosexist Prejudice And Discrimination, James Russell Couch

University of Kentucky Doctoral Dissertations

While the general social climate in the U.S. has become more accepting and tolerant of sexual minority individuals, heterosexist discrimination, prejudice and violence continues to affect LGBT individuals, families and communities. While much research literature exists on the experience of minority stress and the psychological consequences of minority stress on sexual minorities, little research has been produced that examines sexual minority coping. Within the last decade, heteronegativity has been suggested as a possible coping response to heterosexism. The goal of the present study was to understand sexual minority responses to heterosexism (including heteronegativity) in a variety of contexts and circumstances. …

Experimental And Molecular Dynamics Simulation Studies Of Partitioning And Transport Across Lipid Bilayer Membranes, Ravindra Wadhumal Tejwani

University of Kentucky Doctoral Dissertations

Most drugs undergo passive transport during absorption and distribution in the body. It is desirable to predict passive permeation of future drug candidates in order to increase the productivity of the drug discovery process. Unlike drug-receptor interactions, there is no receptor map for passive permeability because the process of transport across the lipid bilayer involves multiple mechanisms. This work intends to increase the understanding of permeation of drug-like molecules through lipid bilayers.

Drug molecules in solution typically form various species due to ionization, complexation, etc. Therefore, species specific properties must be obtained to bridge the experiment and simulations. Due to …

Identification And Characterization Of Host Factors Involved In Tombusvirus Replication, Yi Jiang

University of Kentucky Doctoral Dissertations

Positive strand RNA viruses are intracellular parasites, and their genome replication and infection involves complex virus-host interactions. Therefore, identification of host factors and dissection of their functions during virus replication could facilitate our understanding of the mechanism of virus infection. Those host factors may also provide new targets for viral disease control. Tomato bushy stunt virus (TBSV) has recently become one of the model viruses to study positive strand RNA virus replication and hostvirus interactions. To identify host factors involved in TBSV replication we used yeast as a model host. Co-expression of the replication proteins and a replicon RNA (DI …

Investigation Of Surface Fine Grained Laminae, Streambed, And Streambank Processes Using A Watershed Scale Hydrologic And Sediment Transport Model, Joseph Paul Russo

University of Kentucky Doctoral Dissertations

Sediment transport at the watershed scale in the Bluegrass Region of Kentucky is dominated by surface fine grained laminae, streambed, and streambank erosion; high instream sediment storage; and surface erosion processes. All these processes can be impacted by agricultural, urban, and suburban land-uses as well as hydrologic forcing. Understanding sediment transport processes at the watershed scale is a need for budgeting and controlling sediment pollution, and watershed modeling enables investigation of the cumulative effect of sediment processes and the parameters controlling these processes upon the entire sediment budget for a watershed. Sediment transport is being modeled by coupling the hydrologic …

The Interplay Between The Expression And Functions Of Wnt13 Isoforms During Apoptosis In Bovine Aortic Endothelial Cells, Tao Tang

University of Kentucky Doctoral Dissertations

Wnt proteins are crucial for development/homeostasis by controlling cell fate including apoptosis (Moon RT et al. 1997). Three humanWnt13 isoforms were identified: the secreted Wnt13A, mitochondrial Wnt13B, and nuclear Wnt13C forms; and nuclear Wnt13 had an increased sensitivity to LPS/TNF-induced apoptosis in primary endothelial cells (EC); both Wnt13B and C mRNA contain two start codons (AUG+1 and +74), but the same protein encoded from AUG+74 by Wnt13C was expressed lower than Wnt13B (Struewing IT et al.2006). We hypothesize that during EC apoptosis, the nuclear Wnt13C expression is regulated translationally; nuclear Wnt13 favors apoptosis through regulating the activity/expression of apoptosis-related factors; …

Three Essays On Residential Real Estate Brokerage, Jason S. Beck

University of Kentucky Doctoral Dissertations

The first essay investigates how individual characteristics influence sales outcomes for houses they help transact. It develops hedonic housing models to assess the impacts of agent characteristics such as the level of recent and concurrent agent activity on sales outcomes (price and time on market). This is done in a Multiple Listing Service (MLS) setting using seven years of data obtained from a large Midwestern city. I find evidence that more active listing agents sell homes more quickly, though they do so to the detriment of final sales price. I also find that more listings concurrently held by agents have …

Down-Regulation Of Ppargamma1 Suppresses Cell Growth And Induces Apoptosis In Mcf-7 Breast Cancer Cells, Yekaterina Y. Zaytseva, Xin Wang, R. Chase Southard, Natalie K. Wallis, Michael W. Kilgore

Pharmacology and Nutritional Sciences Faculty Publications

BACKGROUND: Peroxisome proliferator-activated receptor gamma (PPARgamma) is a member of the nuclear hormone receptor superfamily and is highly expressed in many human tumors including breast cancer. PPARgamma has been identified as a potential target for breast cancer therapy based on the fact that its activation by synthetic ligands affects the differentiation, proliferation, and apoptosis of cancer cells. However, the controversial nature of current studies and disappointing results from clinical trials raise questions about the contribution of PPARgamma signaling in breast cancer development in the absence of stimulation by exogenous ligands. Recent reports from both in vitro and in vivo studies …

Educational Spending: Kentucky Vs. Other States, William Hoyt, Christopher Jepsen, Kenneth R. Troske

CBER Research Report

Excerpts from the executive summary:

The passage of the Kentucky Educational Reform Act (KERA) in 1990 had a dramatic impact on the funding of primary and secondary education in the state. The amount of money spent on education increased significantly with the passage of KERA with districts in rural areas of the state experiencing the largest growth in spending (Hoyt, 1999). This has led to a decline in the disparity between rural and urban districts in education spending. However, despite the increase in educational spending, Kentucky still lags behind the typical state in the U.S. in spending per student (Troske, …

Aluminum Bioavailability From Tea Infusion, Robert A. Yokel, Rebecca L. Florence

Pharmaceutical Sciences Faculty Publications

The objective was to estimate oral Al bioavailability from tea infusion in the rat, using the tracer ²⁶Al. ²⁶Al citrate was injected into tea leaves. An infusion was prepared from the dried leaves and given intra-gastrically to rats which received concurrent intravenous ²⁷Al infusion. Oral Al bioavailability (F) was calculated from the area under the ²⁶Al, compared to ²⁷Al, serum concentration × time curves. Bioavailability from tea averaged 0.37%; not significantly different from water (F = 0.3%), or basic sodium aluminum phosphate (SALP) in cheese (F = 0.1 to 0.3%), but greater than acidic SALP …

The North American Transportation Security Center, Steven Douglas Kreis, Michael M. Barclay

Kentucky Transportation Center Research Report

No abstract provided.

Manufactured Aluminum Oxide Nanoparticles Decrease Expression Of Tight Junction Proteins In Brain Vasculature, Lei Chen, Robert A. Yokel, Bernhard Hennig, Michal Toborek

Pharmaceutical Sciences Faculty Publications

Manufactured nanoparticles of aluminum oxide (nano-alumina) have been widely used in the environment; however, their potential toxicity provides a growing concern for human health. The present study focuses on the hypothesis that nano-alumina can affect the blood-brain barrier and induce endothelial toxicity. In the first series of experiments, human brain microvascular endothelial cells (HBMEC) were exposed to alumina and control nanoparticles in dose- and time-responsive manners. Treatment with nano-alumina markedly reduced HBMEC viability, altered mitochondrial potential, increased cellular oxidation, and decreased tight junction protein expression as compared to control nanoparticles. Alterations of tight junction protein levels were prevented by cellular …

Copyright And Permissions: Sometimes They're The Same, Kopana Terry

Library Presentations

No abstract provided.

Agn Dusty Tori. I. Handling Of Clumpy Media, Maia Nenkova, Matthew M. Sirocky, Željko Ivezić, Moshe Elitzur

Physics and Astronomy Faculty Publications

According to unified schemes of active galactic nuclei (AGNs), the central engine is surrounded by dusty, optically thick clouds in a toroidal structure. We have recently developed a formalism that for the first time takes proper account of the clumpy nature of the AGN torus. We now provide a detailed report of our findings in a two-paper series. Here we present our general formalism for radiative transfer in clumpy media and construct its building blocks for the AGN problem-the source functions of individual dusty clouds heated by the AGN radiation field. We show that a fundamental difference from smooth density …

Derivatives Of Mithramycin And Methods Of Making And Uses Thereof, Jürgen Rohr, Lily L. Remsing, Mohammad Ner-E-Alam, Jose A. Salas, Carmen Mendez, Alfredo F. Brana, Ana M. Gonzalez

Pharmaceutical Sciences Faculty Patents

The invention, in one aspect, generally relates to mithramycin derivatives from mutated Streptomyces argillaceus and their production. The invention also relates using the derivatives for the treatment of various diseases. Finally, the invention relates to a mutated Streptomyces argillaceus useful in the production of the mithramycin derivatives.

Forage News [2008-09], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Forage Spokesman Nomination
• KFGC Awards Nomination
• 29th Kentucky Alfalfa Conference - February 19
• How Good Is Your Hay?
• Brassicas and Cereals: High Quality Options for Fall Grazing
• Fall Fertilizer Applications to Hay Fields and Pastures
• USDA Forecasts 2% Less Alfalfa
• Switchgrass for Energy Will Take Time
• Upcoming Events

Cd5 Plays An Inhibitory Role In The Suppressive Function Of Murine Cd4(+) Cd25(+) T(Reg) Cells., Trivikram Dasu, Joseph E Qualls, Halide Tuna, Chander Raman, Donald A Cohen, Subbarao Bondada

Microbiology, Immunology, and Molecular Genetics Faculty Publications

A subset of CD4(+) T cells, the CD4(+) CD25(+) regulatory T (T(reg)) cells in the lymphoid organs and peripheral blood are known to possess suppressive function. Previous in vitro and in vivo studies have indicated that T cell receptor (TCR) signal is required for development of such 'natural regulatory (T(reg)) cells' and for activation of the effector function of CD4(+) CD25(+) regulatory T cells. CD5 is a cell surface molecule present on all T cells and a subtype of B lymphocytes, the B-1 cells, primarily localized to coelomic cavities, Peyer's patches, tonsils and spleen. CD5 acts as a negative regulator …

Cd5 Plays An Inhibitory Role In The Suppressive Function Of Murine Cd4+ Cd25+ TReg Cells, Trivikram Dasu, Joseph E. Qualls, Halide Tuna, Chander Raman, Donald A. Cohen, Subbarao Bondada

Microbiology, Immunology, and Molecular Genetics Faculty Publications

A subset of CD4⁺ T cells, the CD4⁺ CD25⁺ regulatory T (T_reg) cells in the lymphoid organs and peripheral blood are known to possess suppressive function. Previous in vitro and in vivo studies have indicated that T cell receptor (TCR) signal is required for development of such ‘natural regulatory (T_reg) cells’ and for activation of the effector function of CD4⁺ CD25⁺ regulatory T cells. CD5 is a cell surface molecule present on all T cells and a subtype of B lymphocytes, the B-1 cells, primarily localized to coelomic cavities, Peyer's patches, …

Relative Crystallinity Of Plant Biomass: Studies On Assembly, Adaptation And Acclimation, Darby Harris, Seth Debolt

Horticulture Faculty Publications

Plant biomechanical design is central to cell shape, morphogenesis, reproductive performance and protection against environmental and mechanical stress. The cell wall forms the central load bearing support structure for plant design, yet a mechanistic understanding of its synthesis is incomplete. A key tool for studying the structure of cellulose polymorphs has been x-ray diffraction and fourier transform infrared spectroscopy (FTIR). Relative crystallinity index (RCI) is based on the x-ray diffraction characteristics of two signature peaks and we used this technique to probe plant assembly, adaptation and acclimation. Confocal microscopy was used to visualize the dynamics of cellulose synthase in transgenic …

Cytochrome P450 And Uses Of Thereof, Joseph Chappell, Lyle F. Ralston

Pharmaceutical Sciences Faculty Patents

The invention features isolated cytochrome P450 polypeptides and nucleic acid molecules, as well as expression vectors and transgenic plants containing these molecules. In addition, the invention features uses of such molecules in methods of increasing the level of resistance against a disease caused by a plant pathogen in a transgenic plant, in methods for producing altered compounds, for example, hydroxylated compounds, and in methods of producing isoprenoid compounds.

Sumoylation Regulates Lamin A Function And Is Lost In Lamin A Mutants Associated With Familial Cardiomyopathies, Yu-Qian Zhang, Kevin D. Sarge

Molecular and Cellular Biochemistry Faculty Publications

Lamin A mutations cause many diseases, including cardiomyopathies and Progeria Syndrome. The covalent attachment of small ubiquitin-like modifier (SUMO) polypeptides regulates the function of many proteins. Until now, no examples of human disease-causing mutations that occur within a sumoylation consensus sequence and alter sumoylation were known. We show that lamin A is sumoylated at lysine 201 and that two lamin A mutants associated with familial dilated cardiomyopathy, E203G and E203K, exhibit decreased sumoylation. E203 occupies the conserved +2 position in the sumoylation consensus Psi KXE. Lamin A mutants E203G, E203K, and K201R all exhibit a similar aberrant subcellular localization and …

Evaluation Of Kentucky’S “Click It Or Ticket” 2008 Campaign, Kenneth R. Agent, Eric R. Green, Ronald E. Langley

Kentucky Transportation Center Research Report

The objective of this report was to document the results of the “Click It or Ticket” 2008 campaign in Kentucky. The campaign involved a combination of earned media, paid media, and enforcement. The earned media started about three weeks prior to Memorial Day 2008 and continued through the enforcement period. The paid media campaign was for the two weeks prior to Memorial Day while the enforcement period was a two-week period including the week before and the week of Memorial Day.

The evaluation of the campaign included documenting the activities associated with the program (publicity and enforcement) and evaluating the …

A Polyadenylation Factor Subunit Implicated In Regulating Oxidative Signaling In Arabidopsis Thaliana, Jingxian Zhang, Bala Subrahmanyam Addepalli, Kil-Young Yun, Arthur G. Hunt, Ruqiang Xu, Suryadevara Rao, Qingshun Q. Li, Deane L. Falcone

Kentucky Tobacco Research and Development Center Faculty Publications

BACKGROUND: Plants respond to many unfavorable environmental conditions via signaling mediated by altered levels of various reactive oxygen species (ROS). To gain additional insight into oxidative signaling responses, Arabidopsis mutants that exhibited tolerance to oxidative stress were isolated. We describe herein the isolation and characterization of one such mutant, oxt6.

METHODOLOGY/PRINCIPAL FINDINGS: The oxt6 mutation is due to the disruption of a complex gene (At1g30460) that encodes the Arabidopsis ortholog of the 30-kD subunit of the cleavage and polyadenylation specificity factor (CPSF30) as well as a larger, related 65-kD protein. Expression of mRNAs encoding Arabidopsis CPSF30 alone was able to …

Adaptation And Mal-Adaptation To Ambient Hypoxia; Andean, Ethiopian And Himalayan Patterns, Guoqiang Xing, Clifford Qualls, Luis Huicho, Maria Rivera-Ch, Tsering Stobdan, Marat Slessarev, Eitan Prisman, Shoji Ito, Hong Wu, Angchuk Norboo, Diskit Dolma, Moses Kunzang, Tsering Norboo, Jorge L Gamboa, Victoria E. Claydon, Joseph Fisher, Guta Zenebe, Amha Gebremedhin, Roger Hainsworth, Ajay Verma, Otto Appenzeller

Physiology Faculty Publications

The study of the biology of evolution has been confined to laboratories and model organisms. However, controlled laboratory conditions are unlikely to model variations in environments that influence selection in wild populations. Thus, the study of "fitness" for survival and the genetics that influence this are best carried out in the field and in matching environments.

Therefore, we studied highland populations in their native environments, to learn how they cope with ambient hypoxia. The Andeans, African highlanders and Himalayans have adapted differently to their hostile environment.

Chronic mountain sickness (CMS), a loss of adaptation to altitude, is common in the …

Compost For Steep Slope Erosion, Sudhir Palle, Stephen F. Higgins, Theodore Hopwood Ii

Kentucky Transportation Center Research Report

This study was initiated to develop guidelines for maintenance erosion control measures for steep slopes. The study focused on evaluating and monitoring KY-31 fescue germination rates using two media treatments 1) 100 percent by weight compost and 2) 70 percent compost with 30 percent soil) with different seeding rates on a pilot project to establish vegetation on slopes with grades of 3:1 or steeper.

The pilot program demonstrated that weed infestation and erosion problems on slopes can be minimized by use of properly formulated compost with sufficient seed. It also showed that the seeding rate of 1 lb/1,000 ft2 slope …

Agn Dusty Tori. Ii. Observational Implications Of Clumpiness, Maia Nenkova, Matthew M. Sirocky, Robert Nikutta, Željko Ivezić, Moshe Elitzur

Physics and Astronomy Faculty Publications

Clumpy torus models with N₀ ~ 5–15 dusty clouds along radial equatorial rays successfully explain AGN infrared observations. The dust has standard Galactic composition, with individual cloud optical depth τ_V ~ 30–100 at visual. The models naturally explain the observed behavior of the 10 μm silicate feature, in particular the lack of deep absorption features in AGNs of any type, and can reproduce the weak emission feature tentatively detected in type 2 QSOs. The clouds' angular distribution must have a soft edge, e.g., Gaussian, and the radial distribution should decrease as 1/r or 1/r^{2 …}

Arabidopsis Mrna Polyadenylation Machinery: Comprehensive Analysis Of Protein-Protein Interactions And Gene Expression Profiling, Arthur G. Hunt, Ruqiang Xu, Balasubrahmanyam Addepalli, Suryadevara Rao, Kevin P. Forbes, Lisa R. Meeks, Denghui Xing, Min Mo, Hongwei Zhao, Amrita Bandyopadhyay, Lavanya Dampanaboina, Amanda Marion, Carol Von Lanken, Qingshun Quinn Li

Plant and Soil Sciences Faculty Publications

BACKGROUND: The polyadenylation of mRNA is one of the critical processing steps during expression of almost all eukaryotic genes. It is tightly integrated with transcription, particularly its termination, as well as other RNA processing events, i.e. capping and splicing. The poly(A) tail protects the mRNA from unregulated degradation, and it is required for nuclear export and translation initiation. In recent years, it has been demonstrated that the polyadenylation process is also involved in the regulation of gene expression. The polyadenylation process requires two components, the cis-elements on the mRNA and a group of protein factors that recognize the cis-elements and …

Recombinant Stokesia Epoxygenase Gene, David Hildebrand, Tomoko Hatanaka

Plant and Soil Sciences Faculty Patents

A chimeric gene comprising an isolated nucleic acid molecule encoding a delta 12-fatty acid epoxygenase enzyme and transgenic plants containing the chimeric gene are described. Expression of the chimeric delta 12-epoxygenase gene leads to altered levels of fatty acids in transformed cells.

"Historical Forgetfulness" In Post-Unification Italy: Black African Migration In Politics And Performance, Raffaele Furno

disClosure: A Journal of Social Theory

No abstract provided.