Digital Commons Network^™

Pollution Knowledge And Urban Water Politics In The Ganges River Basin (India), Sya Buryn Kedzior

University of Kentucky Doctoral Dissertations

Millions of people rely upon the Ganges River as a source of water provision and a site of disposal for sewage, solid waste, agricultural runoff and industrial effluent. The river is also a goddess in the Hindu pantheon who is worshipped for her purificatory powers, despite water quality levels that fall far short of standards for use in bathing, washing, and drinking. In recent years, a number of non-governmental organizations (NGOs) have formed to oppose both pollution of the river and the failure of state-run pollution abatement programs. They are joined by an increasingly frequent number of seemingly spontaneous protests …

Evaluation Of The Physicochemical Properties And Stability Of Solid Lipid Nanoparticles Designed For The Delivery Of Dexamethasone To Tumors, Melissa Howard

University of Kentucky Doctoral Dissertations

Pre-clinical and clinical trials suggest that pre-treatment with dexamethasone (Dex) may facilitate enhanced uptake of subsequently administered chemotherapeutic agents. To reduce the side effects associated with systemic administration of Dex, solid lipid nanoparticles (SLNs) containing dexamethasone palmitate (Dex-P) were prepared as a means of achieving tumor-targeted drug delivery. These studies were aimed at evaluating the physicochemical properties and both the physiological and storage stability of the SLNs.

SLNs were prepared using nanotemplate engineering technology. Stearyl alcohol (SA) was used as the lipid phase with Brij^® 78 and Polysorbate 60 as surfactants and PEG6000 monostearate as a long-chain PEGylating agent. …

Genotoxin-Induced Acetylation Of The Werner Syndrome Protein (Wrn) And Effect On Its Dna Metabolic Function, Enerlyn Meliza Lozada Santiago

University of Kentucky Doctoral Dissertations

Loss of function of the WRN protein causes the genetic disorder Werner Syndrome that is characterized by increased cancer and premature aging. WRN belongs to the RecQ helicase family that plays key roles in preventing genome instability. In response to treatment with genotoxins, WRN is subject to post-translational modification. The relationship of post-translational modification of WRN with its function in DNA metabolism is unknown. There is accumulating evidence suggesting that WRN contributes to the maintenance of genomic integrity through its involvement in DNA replication. Consistent with this notion, WS cells are sensitive to DNA replication inhibitors and DNA damaging agents …

The Return Of The Black Bear To Eastern Kentucky: Conflict And Tolerance Between People And Wildlife, Hannah B. Harris

University of Kentucky Doctoral Dissertations

The black bear (Ursus americanus) has returned to Kentucky and is now part of a reproducing population in the southeastern Cumberland Mountain region. The broad objective of this project was to examine the interactions between people and bears, with the ultimate goal of improving bear management in a way that addresses stakeholder concerns. Using interviews of regional stakeholders, participant observation, and media reports collected between summer 2003 and fall 2006, I investigated how the presence of black bears in Harlan and Letcher counties in Kentucky has had an impact on area residents. I complemented this information with observations …

Appalachian Bridges To The Baccalaureate: Institutional Perceptions Of Community College Transfer Success, Christopher M. Phillips

University of Kentucky Doctoral Dissertations

Statement of the problem. Appalachian community colleges are dealing with a dynamic transfer policy environment and implementing practices that either foster or impede transfer student success. The problem in this dissertation is to discern how Appalachian community colleges are making sense of transfer policy changes and conducting practices to address student transfer success. Although individual factors must be considered by community colleges, they often are out of the control of the institution. This study focused on the institutional factors, including the ways that organizational structures and transfer policies contribute to the success of a community college’s transfer program.

Design. This …

Role Of Angiotensin Converting Enzyme 2 (Ace2) In Obesity-Associated Hypertension, Manisha Gupte

University of Kentucky Doctoral Dissertations

The purpose of this research was to determine whether adipocytes express ACE2 and its role in obesity-associated hypertension with diet-induced obesity.

To determine if ACE2 was expressed in adipose tissue and its regulation in the setting of diet-induced obesity, we fed male mice either a low fat (LF) or high fat (HF) diet acutely (1 week) or chronically ( 4 months). We demonstrated that ACE2 was regulated specifically in adipose tissue with consumption of a HF diet. However, with chronic HF feeding adipose ACE2 was dysregulated resulting in activation of the systemic RAS and increased blood pressure.

To determine the …

Factors Associated With Tobacco Use Among Rural And Urban Pregnant Women, Whitney Jeanne Katirai

University of Kentucky Doctoral Dissertations

The purpose of this study was to investigate the influences of smoking on rural and urban pregnant women. More specifically, the variables of the knowledge of health effects, health provider recommendations, subscores from the Health Belief Model (HBM), and social support were explored in relation to the smoking behavior of pregnant women. A secondary purpose was to investigate the accuracy of self-reported smoking during pregnancy using biochemical validation. Pregnant women (N=71) completed an anonymous questionnaire, designed by the researcher, to identify variables that predicted smoking for urban and rural women. Participants also gave a saliva sample for cotinine testing.

Approximately …

Advanced Studies On Series Impedance In Waveguides With An Emphasis On Source And Transfer Impedance, Jinghao Liu

University of Kentucky Doctoral Dissertations

Series impedances, including source and transfer impedances, are commonly used to model a variety of noise sources and noise treatment elements in duct systems. Particle velocity is assumed to be constant on the plane where the series impedances are defined. The research reported herein details investigations into measuring source and transfer impedance. Especially, the measurement and prediction of the transfer impedance of micro-perforated panel (MPP) absorbers is considered.

A wave decomposition method for measuring source impedance and source strength was developed that was purely based on acoustic concepts instead of the equivalent circuit analysis. The method developed is a two-load …

Fabrication And Characterization Of Mesoscale Protein Patterns Using Atomic Force Microscopy (Afm), Pei Gao

University of Kentucky Doctoral Dissertations

A versatile AFM local oxidation lithography was developed for fabricating clean protein patterns ranging from nanometer to sub-millimeter scale on octadecyltrichlorosilane (OTS) layer of Si (100) wafer. This protein patterning method can generate bio-active protein pattern with a clean background without the need of the anti-fouling the surface or repetitive rinsing.

As a model system, lysozyme protein patterns were investigated through their binding reactions with antibodies and aptamers by AFM. Polyclonal anti-lysozyme antibodies and anti-lysozyme aptamer are found to preferentially bind to the lysozyme molecules on the edge of a protein pattern before their binding to the interior ones. It …

Interactions And Effects Of Biomolecules On Au Nanomaterial Surfaces, Manish Sethi

University of Kentucky Doctoral Dissertations

Au nanoparticles are increasingly being used in biological applications. Their use is of interest based upon their unique properties that are achieved at the nanoscale, which includes strong optical absorbances that are size and aggregation state dependent. Such absorbances can be used in sensitive chemical/biological detection schemes where bioligands can be directly attached to the nanoparticle surface using facile methods. Unfortunately, a number of complications persist that prevent their wide-scale use. These limitations include minimal nanoparticle stability in biological-based media of high ionic strength, unknown surface functionalization effects using simple biomolecules, and determining the binding motifs of the ligands to …

Identification Of Activities Involved In Cag/Ctg Repeat Instability, Nelson Lap Shun Chan

University of Kentucky Doctoral Dissertations

CAG/CTG repeat instability is associated with at least 14 neurological disorders, including Huntington’s disease and Myotonic dystrophy type 1. In vitro and in vivo studies have showed that CAG/CTG repeats form a stable hairpin that is believed to be the intermediate for repeat expansion and contraction.

Addition of extra DNA is essential for repeat expansion, so DNA synthesis is one of the keys for repeat expansion. In vivo studies reveal that 3’ CTG slippage with subsequent hairpin formation (henceforth called the 3’ CTG slippage hairpin) occurs during DNA synthesis. It is proposed that hairpin tolerance machinery is activated because prolonged …

Effect Of Azithromycin On Macrophage Phenotype During Pulmonary Infections And Cystic Fibrosis, Theodore James Cory

University of Kentucky Doctoral Dissertations

Azithromycin improves clinical outcomes in patients with cystic fibrosis (CF), specifically in patients infected with Pseudomonas aeruginosa. Azithromycin shifts macrophage programming away from a pro-inflammatory classical (M1) phenotype, and towards an anti-inflammatory alternative (M2) phenotype; however, little is known about this mechanism, nor of its impact upon immune response to pulmonary infection. We set out to determine the mechanism by which azithromycin is able to alter macrophage phenotype, and assess the effect of azithromycin induced macrophage polarization on inflammation during pulmonary infections.

Utilizing macrophage cell culture, we found that azithromycin increased IKKβ, a signaling molecule in the NFκB pathway, which …

Three Dimensional Modeling And Animation Of Facial Expressions, Alice J. Lin

University of Kentucky Doctoral Dissertations

Facial expression and animation are important aspects of the 3D environment featuring human characters. These animations are frequently used in many kinds of applications and there have been many efforts to increase the realism. Three aspects are still stimulating active research: the detailed subtle facial expressions, the process of rigging a face, and the transfer of an expression from one person to another. This dissertation focuses on the above three aspects.

A system for freely designing and creating detailed, dynamic, and animated facial expressions is developed. The presented pattern functions produce detailed and animated facial expressions. The system produces realistic …

Appalachian Bridges To The Baccalaureate: Mattering Perceptions And Transfer Persistence Of Low-Income, First-Generation Community College Students, Michelle Dykes

University of Kentucky Doctoral Dissertations

Too few community college students who intend to transfer and earn a baccalaureate degree actually do. Further, low-income and first-generation college students are overrepresented at community colleges. Education is considered a means of social and economic mobility for low-income, first-generation students; therefore, retaining this population through baccalaureate attainment is a critical issue. Because of the multitude of obstacles these students must conquer, it is crucial to implement effective strategies for improving transfer rates.

This dissertation has three components: (1) companion research study, (2) individual research study, and (3) reflective essay examining pretesting and telephone-administered survey methods. The companion study was …

Role Of Lipids In Tombusvirus Replication, Monika Sharma

University of Kentucky Doctoral Dissertations

Positive-strand RNA virus group are the most abundant among viruses affecting plants and animals. To successfully achieve replication, these viruses usurp or co-opt host proteins. To facilitate the discovery of host factors involved in Tomato bushy stunt virus (TBSV), yeast has been developed as a surrogate model host. Genome-wide approaches covering 95% of yeast genes, has revealed approximately hundred factors that could affect virus replication. Among the identified host factors, there are fourteen yeast genes, which affect/regulate lipid metabolism of the host.

One of the identified host gene is ERG25, which is an important factor for sterol biosynthesis pathway, affecting …

Appalachian Bridges To The Baccalaureate: How Community Colleges Affect Transfer Success, Amber K. Decker

University of Kentucky Doctoral Dissertations

Statement of the problem. Too few community college students who intend to transfer and earn a baccalaureate degree actually do. This is a problem because postsecondary education is a key factor in economic mobility, and community colleges enroll a disproportionate number of nontraditional, part-time and low-income students. Although individual factors must be considered by community colleges, they often are out of the control of the institution. This study focused on the institutional factors, including the ways that organizational structures contribute to the success of a community college’s transfer program.

Design. This companion study was conducted by a four-member research team. …

Appalachian Bridges To The Baccalaureate: The Influence Of Multiple Roles And Cultural Norms On The Baccalaureate Persistence Of Location-Bound Appalachian Women, Nancy Coldiron Preston

University of Kentucky Doctoral Dissertations

Too few Kentucky community college students transfer and persist to earn baccalaureate degrees. This is particularly true in Appalachia Kentucky which has a high rate of poverty and a low rate of baccalaureate attainment. Scholars and economists agree that the fastest way to decrease poverty within a geographical region is to increase the educational level of the citizens. Policy makers in the Commonwealth have established a goal of doubling the number of baccalaureate holders within the state by 2020.

This study is framed by a collaborative study which examined the ways in which institutional and student characteristics impact the pathway …

Disjuncture Among Classic Period Cultural Landscapes In The Tuxtla Mountains, Southern Veracruz, Mexico, Wesley Durrell Stoner

University of Kentucky Doctoral Dissertations

Teotihuacan was the most influential city in the Classic Mesoamerican worldsystem. Like other influential cities in the ancient world, however, Teotihuacan did not homogenously affect the various cultural landscapes that thrived in Mesoamerica during the Classic period (300-900 CE). Even where strong central Mexican influences appear outside the Basin of Mexico, the nature, extent, and strength of these influences are discontinuous over time and space. Every place within the Classic Mesoamerican landscape has a unique Teotihuacan story. In the Tuxtla Mountains of southern Veracruz, Mexico, Matacapan, located in the Catemaco Valley, drew heavily upon ideas and symbols fostered at Teotihuacan, …

Nation, Fantasy, And Mimicry: Elements Of Political Resistance In Postcolonial Indian Cinema, Aparajita Sengupta

University of Kentucky Doctoral Dissertations

In spite of the substantial amount of critical work that has been produced on Indian cinema in the last decade, misconceptions about Indian cinema still abound. Indian cinema is a subject about which conceptions are still muddy, even within prominent academic circles. The majority of the recent critical work on the subject endeavors to correct misconceptions, analyze cinematic norms and lay down the theoretical foundations for Indian cinema. This dissertation conducts a study of the cinema from India with a view to examine the extent to which such cinema represents an anti-colonial vision. The political resistance of Indian films to …

Androgen Increases Angiotensin Receptor Type 1a On Smooth Muscle Cells To Promote Angiotensin Ii-Induced Abdominal Aortic Aneurysms, Xuan Zhang

University of Kentucky Doctoral Dissertations

The purpose of this study was to determine whether androgen promotes AT1aR expression on smooth muscle to confer high prevalence of AngII-induced AAAs in hyperlipidemic mice. In addition, we also investigate the role of androgen in the progression of established AngII-induced AAAs.

First, we sought to examine the role of endogenous androgen in the growth of established AngII-induced AAAs. By castrating male mice, we demonstrated that removal of endogenous androgen significantly decreased the progressive lumen dilation of established AngII-induced AAAs in male ApoE-/- mice, but had no effect on external AAA diameters. These results suggest that androgen contributes to the …

The Role Of The Nr4a Orphan Nuclear Receptor Nor1 In Vascular Cells And Atherosclerosis, Yue Zhao

University of Kentucky Doctoral Dissertations

The neuron-derived orphan receptor 1 (NOR1) belongs to the NR4A nuclear receptor subfamily. As an immediate early response gene, NOR1 is rapidly induced by a broad spectrum of physiological and pathological signals. Functional studies demonstrate NOR1 as a constitutively active ligand-independent nuclear receptor whose transcriptional activity is dependent on both expression level and posttranslational modifications. To date, an increasing number of studies have demonstrated a pivotal role of NOR1 in the transcriptional control of metabolism and the development of cardiovascular diseases.

In this dissertation, we demonstrate NOR1 expression in endothelial cells and sub-endothelial cells of human atherosclerotic lesions. In response …

Technologies Of Apprehension: The Family, Law, Security, And Geopolitics In Us Noncitizen Family Detention Policy And Practice, Lauren Leigh Martin

University of Kentucky Doctoral Dissertations

This dissertation examines how US immigrant family detention policy emerged from reinvigorated border security priorities, immigration policing practices, and international migration flows. Based on a qualitative mixed methods approach, the research traces how discourses of threat, vulnerability, and safety produce detainable child and parent subjects that displace “the family” as a legal entity. I show that immigration law relies on specific kinds of geographical knowledge, producing what I call the ‘geopolitics of vulnerability.’ More broadly, I analyze how current immigration enforcement practices work at local, national, and international scales, so that detention deters future migration as much as it penalizes …

Understanding Interactive Experiences: Perceived Interactivity And Presence With And Without Other Avatars In The Online Virtual World Second Life, Jennifer Lynn Robinette

University of Kentucky Doctoral Dissertations

Interactivity research lacks consensus regarding the qualities and consequences of interactive experiences. Empirical proof is needed to substantiate the numerous interactivity theories and provide direction for new media technology developers. Specifically, there is a shortage of research on differences between user experiences of interactivity when technology enables communication versus when it does not. In addition, interactivity research is often confounded by the construct of presence.

This study’s objectives included: 1) identifying qualities associated with interactive experiences; 2) disambiguating the constructs of interactivity and presence; and 3) developing a measure of perceived interactivity for VW research. The experimental design measured perceived …

Effects Of Corticosterone And Ethanol Co-Exposure On Hippocampal Toxicity: Potential Role For The Nmda Nr2b Subunit, Tracy Renee Butler

University of Kentucky Doctoral Dissertations

Chronic ethanol (EtOH) exposure produces neuroadaptations within the NMDA receptor system and alterations in HPA axis functioning that contribute to neurodegeneration during ethanol withdrawal (EWD). Chronic EtOH exposure and EWD, as well as corticosteroids, also promote increased synthesis and release of polyamines, which allosterically potentiate NMDA receptor open-channel time at the NR2B subunit. The current studies investigated effects of 10 day EtOH and corticosterone (CORT) co-exposure on toxicity during EWD in rat organotypic hippocampal slice cultures, and alterations in function and/or density of the NR2B subunit of the NMDA receptor that may mediate CORT-potentiation of toxicity during EWD. We hypothesized …

Texting In The Presence Of Others: The Use Of Politeness Strategies In Conversation, Jennifer Ann Maginnis

University of Kentucky Doctoral Dissertations

The following study used politeness theory to explore the impact of simultaneously engaging in a face to face conversation and a text message conversation. Specifically the study used Brown and Levinson’s (1978, 1987) five original politeness strategies to see whether strategy choice (in the face to face conversation) impacts the face threat present in engaging in multiple conversations. Multivariate analysis of covariance was used to understand the impact different politeness strategies had on the following variables: conversational appropriateness, relational/social appropriateness, immediacy, attentiveness, and politeness. Findings show that when a face to face partner ignores (no verbal/nonverbal politeness) a text message …

Combinatorial Biosynthetic Derivatization Of The Antitumoral Agent Gilvocarcin V, Micah Douglas Shepherd

University of Kentucky Doctoral Dissertations

Gilvocarcin V (GV), the principal product of Streptomyces griseoflavus Gö 3592 and other Streptomyces spp., is the most prominent member of a distinct class of antitumor antibiotics that share a polyketide derived coumarin-based aromatic core. GV and other members of this class including polycarcin V from Streptomyces polyformus, often referred to as gilvocarcin-like aryl C-glycosides, are particularly interesting because of their potent bactericidal, virucidal and antitumor activities at low concentrations while maintaining low in vivo toxicity. Although the precise molecular mechanism of GV bioactivity is unknown, gilvocarcin V has been shown to undergo a photoactivated [2+2] cycloaddition of …

Changing The Way We Do Things Around Here: Strategies Presidents Use For Creating Organizational Change In Higher Education, Jared N. Tippets

University of Kentucky Doctoral Dissertations

As society changes, so must higher education. Therefore, colleges and universities and the presidents leading them must adapt to survive in today’s highly competitive marketplace. What must an institution do to stay abreast of the changes? Once the necessary changes are identified, what processes are used by a president of a college or university to go about deliberately creating change on campus? By understanding how to create planned change, leaders within higher education will be able to better ensure that their institutions remain relevant in today’s rapidly changing environment. For many colleges and universities, their ultimate survival may depend on …

Microneedle-Assisted Transdermal Delivery Of Naltrexone Species: In Vitro Permeation And In Vivo Pharmacokinetic Studies, Mikolaj Milewski

University of Kentucky Doctoral Dissertations

Naltrexone (NTX) is a drug used primarily in the management of alcohol dependence and opioid dependence. Based on several drawbacks associated with the oral and injectable intramuscular dosage forms of naltrexone currently available on the market, there is substantial interest in delivering naltrexone transdermally. Although naltrexone does not permeate skin at the rate sufficient to reach therapeutic plasma concentrations in humans, novel flux enhancement methods such as microneedles help address this challenge. Earlier work in humans has demonstrated that the use of microneedles achieves plasma concentrations in the lower end of expected therapeutic values. Further flux enhancement is desired to …

Strategies For Targeting Lentiviral Vectors, Christopher Matthew Trimby

University of Kentucky Doctoral Dissertations

Lentiviral gene therapy has held great promise for treating a wide range of neurological disorders due to its ability to stably integrate into the genome of nondividing cells like neurons, in addition to dividing cells. The nervous system is a complex and highly heterogeneous system, and while a therapeutic intervention may have beneficial effects in one population of cells it may have severe side effects in another. For this reason, specific targeting of lentiviral vectors is crucial for their ultimate utility for research and clinical research use.

Two different approaches for focusing the targeting of lentiviral vectors were employed in …

Solution Phase And Membrane Immobilized Iron-Based Free Radical Reactions: Fundamentals And Applications For Water Treatment, Scott Romak Lewis

University of Kentucky Doctoral Dissertations

Membrane-based separation processes have been used extensively for drinking water purification, wastewater treatment, and numerous other applications. Reactive membranes synthesized through functionalization of the membrane pores offer enhanced reactivity due to increased surface area at the polymer-solution interface and low diffusion limitations. Oxidative techniques utilizing free radicals have proven effective for both the destruction of toxic organics and non-environmental applications. Most previous work focuses on reactions in the homogeneous phase; however, the immobilization of reactants in membrane pores offers several advantages. The use of polyanions immobilized in a membrane or chelates in solution prevents ferric hydroxide precipitation at near-neutral pH, …