Digital Commons Network^™

Calpain-2 Compensation Promotes Angiotensin Ii-Induced Ascending And Abdominal Aortic Aneurysms In Calpain-1 Deficient Mice, Venkateswaran Subramanian, Jessica J. Moorleghen, Anju Balakrishnan, Deborah A. Howatt, Athar H. Chishti, Haruhito A. Uchida

Saha Cardiovascular Research Center Faculty Publications

BACKGROUND AND OBJECTIVE: Recently, we demonstrated that angiotensin II (AngII)-infusion profoundly increased both aortic protein and activity of calpains, calcium-activated cysteine proteases, in mice. In addition, pharmacological inhibition of calpain attenuated AngII-induced abdominal aortic aneurysm (AA) in mice. Recent studies have shown that AngII infusion into mice leads to aneurysmal formation localized to the ascending aorta. However, the precise functional contribution of calpain isoforms (-1 or -2) in AngII-induced abdominal AA formation is not known. Similarly, a functional role of calpain in AngII-induced ascending AA remains to be defined. Using BDA-410, an inhibitor of calpains, and calpain-1 genetic deficient mice, …

Predictors Of Online And Offline Sexual Activities And Behaviors Among Adolescents, Anna Ševčíková, Alexander T. Vazsonyi, Janelle Širůček, Štěpán Konečný

Psychology Faculty Publications

Despite the fact that many adolescents spend much time on the Internet, it is unknown who engages in sexually related online activities (SROA) and how these affect adolescent sexual development. The present longitudinal study on 323 adolescents (51.1% girls) aimed to explore how peer attachment processes predicted both SROA and offline sexual behaviors at the age of 17, while also considering puberty and prior offline sexual experiences in order to elucidate potential similarities or differences. Findings based on hierarchical, binary logistic regression analyses revealed that SROA were predicted by alienation attachment to peers (OR=3.36, p<0.05), puberty (OR=1.03, p<0.05), and prior SROA (OR=0.56, p<0.001), while only previous offline sexual experiences at the age of 15 increased the likelihood of offline sexual behaviors at the age of 17 (OR=6.04, p<0.001). Study findings indicate that the Internet provides an additional context for acquiring sexual experiences during adolescence.

Autophagy Is Involved In Oligodendroglial Precursor-Mediated Clearance Of Amyloid Peptide, Wenxia Li, Yifen Tang, Zhiqin Fan, Ya Meng, Guang Yang, Jia Luo, Zun-Ji Ke

Internal Medicine Faculty Publications

BACKGROUND: Accumulation of β-amyloid peptides is an important hallmark of Alzheimer's disease (AD). Tremendous efforts have been directed to elucidate the mechanisms of β-amyloid peptides degradation and develop strategies to remove β-amyloid accumulation. In this study, we demonstrated that a subpopulation of oligodendroglial precursor cells, also called NG2 cells, were a new cell type that can clear β-amyloid peptides in the AD transgene mice and in NG2 cell line.

RESULTS: NG2 cells were recruited and clustered around the amyloid plaque in the APPswe/PS1dE9 mice, which is Alzheimer's disease mouse model. In vitro, NG2 cell line and primary NG2 cells engulfed …

Assessing The Bioconfinement Potential Of A Nicotiana Hybrid Platform For Use In Plant Molecular Farming Applications, J. Hollis Rice, Richard E. Mundell, Reginald J. Millwood, Orlando D. Chambers, C. Neal Stewart, H. Maelor Davies

Kentucky Tobacco Research and Development Center Faculty Publications

BACKGROUND: The introduction of pharmaceutical traits in tobacco for commercial production could benefit from the utilization of a transgene bioconfinement system. It has been observed that interspecific F1Nicotiana hybrids (Nicotiana tabacum × Nicotiana glauca) are sterile and thus proposed that hybrids could be suitable bioconfined hosts for biomanufacturing. We genetically tagged hybrids with green fluorescent protein (GFP), which was used as a visual marker to enable gene flow tracking and quantification for field and greenhouse studies. GFP was used as a useful proxy for pharmaceutical transgenes.

RESULTS: Analysis of DNA content revealed significant genomic downsizing of the hybrid relative to …

Bridging Storytelling Traditions With Digital Technology, Melany Cueva, Regina Kuhnley, Laura J. Revels, Katie Cueva, Mark B. Dignan, Anne P. Lanier

Internal Medicine Faculty Publications

Objective. The purpose of this project was to learn how Community Health Workers (CHWs) in Alaska perceived digital storytelling as a component of the “Path to Understanding Cancer” curriculum and as a culturally respectful tool for sharing cancer-related health messages.

Design. A pre-course written application, end-of-course written evaluation, and internet survey informed this project.

Methods. Digital storytelling was included in seven 5-day cancer education courses (May 2009–2012) in which 67 CHWs each created a personal 2–3 minute cancer-related digital story. Participant-chosen digital story topics included tobacco cessation, the importance of recommended cancer screening exams, cancer survivorship, loss, …

Sex Trafficking Of Minors In Kentucky, Jennifer Cole, Elizabeth Anderson

Center on Trauma and Children Reports

From executive summary:

Findings presented in this report are from telephone surveys conducted from July 2012 through April 2013 with 323 professionals who worked in agencies that serve at-risk youth and/or crime victims across Kentucky. Respondents were from all geographic and demographic communities in Kentucky, with the highest number serving Bluegrass metropolitan communities including Louisville, Lexington, and Northern Kentucky.

Smoking On The Margins: An Equity Analysis Of Vancouver's Outdoor Smoke-Free Policy In Parks And On Beaches, Chizimuzo T.C. Okoli, Ann Pederson

Nursing Presentations

Background: Increasingly, jurisdictions worldwide are addressing smoking restrictions in outdoor public spaces to reduce secondhand smoke exposure, discourage youth initiation, enhance cessation, and reduce environmental hazards (i.e., fire and cigarette-related litter). However, there is little research on the policy context and health-equity impact of such policies to help guide wider implementation efforts.

Objectives: On September 1, 2010, the Board of Parks and Recreation in Vancouver, Canada, introduced a smoke-free bylaw for the city’s parks and beaches. The Smoking on the Margins project is examining the policy context and potential health-equity impact of this bylaw.

Methods: Applying critical multiplism and equity-focused …

Library Services For Creating And Publishing Student Research Journals, Adrian K. Ho

Library Faculty and Staff Publications

Theme: Planning and implementing student research journals

Highlighted Services: Journal consulting, publishing and technical support

Resources: Sample journal staff organization models, checklists to support planning, staffing, and production

The Use Of Co-Culturing In Solid Substrate Cultivation And Possible Solutions To Scientific Challenges, Wanying Yao, Sue E. Nokes

Biosystems and Agricultural Engineering Faculty Publications

This perspective systematically summarizes the use of solid substrate co‐cultures in agriculture, food, plant, and industrial biotechnology applications. The summarization is organized by organism, i.e. fungus, bacteria, yeast and then co‐cultivation of either two or three organisms. Generally, in solid substrate co‐culture, the organisms synergistically penetrate and degrade the solid substrate, thereby increasing product yield and productivity over a monoculture. Efforts to increase co‐culture performance include optimizing process parameters (pH, temperature, moisture, and oxygen demand) and defining the acceptable types of substrate. Scientific challenges exist in understanding the interactions between microbial stains, such as viability, suite of products, and bio‐transformations. …

What’S In A Username? Finding Local Health Departments On Twitter, Jenine K. Harris, Ryan C. Maier, Nina Jolani

Frontiers in Public Health Services and Systems Research

Social media platforms such as Twitter may be useful for local health departments (LHDs) in providing the essential service of educating and informing constituents.¹ However, health departments have relatively few Twitter followers overall.¹ One of the challenges that may be associated with following LHDs on Twitter is knowing how to find an LHD Twitter feed. With no suggested or required conventions for LHDs adopting social media, practitioners are left to independently develop their name and description, resulting in much variety. This report examines the names and descriptions for LHDs using Twitter and uses the Twitter people search function …

Ringers Revisited, Richard H. Underwood

Law Faculty Scholarly Articles

In this short essay, Professor Underwood addresses an important development in the law dealing with eyewitness testimony and the New Jersey case of State v. Henderson. He gets at the subject by looking back to a 1950s television play starring fellow Kentucky resident, William Shatner. However, in this particular instance, William Shatner would not change the world.

Metal-Based Nanoparticle Interactions With The Nervous System: The Challenge Of Brain Entry And The Risk Of Retention In The Organism, Robert A. Yokel, Eric A. Grulke, Robert C. Macphail

Pharmaceutical Sciences Faculty Publications

This review of metal-based nanoparticles focuses on factors influencing their distribution into the nervous system, evidence they enter brain parenchyma, and nervous system responses. Gold is emphasized as a model metal-based nanoparticle and for risk assessment in the companion review. The anatomy and physiology of the nervous system, basics of colloid chemistry, and environmental factors that influence what cells see are reviewed to provide background on the biological, physical–chemical, and internal milieu factors that influence nervous system nanoparticle uptake. The results of literature searches reveal little nanoparticle research included the nervous system, which about equally involved in vitro and in …

Southeastern Law Librarian Summer 2013, Seaall

Newsletters

No abstract provided.

Global Kentucky (Summer 2013), University Of Kentucky International Center

Global Kentucky

No abstract provided.

A Collaborative Professional Development Approach To Improving Student Outcomes, Jillian Starman, Ann Larson, Eve Proffitt, Thomas R. Guskey, Xin Ma

Educational, School, and Counseling Psychology Faculty Publications

The purpose of this article is to analyze the strategy used to develop a collaborative professional development process that focused on the implementation of the common core state standards. In addition, the author will report findings, and discuss the effectiveness of the initiative for postsecondary faculty. Faculty can verify that not every student comes to a two or four year college program prepared to succeed in credit bearing coursework. To facilitate improvement of student success, states across the country collaborated with teachers, researchers and leading experts to design and develop the common core state standards. The standards were developed to …

Astrocyte-Specific Overexpression Of Insulin-Like Growth Factor-1 Protects Hippocampal Neurons And Reduces Behavioral Deficits Following Traumatic Brain Injury In Mice, Sindhu K. Madathil, Shaun W. Carlson, Jennifer M. Brelsfoard, Ping Ye, A Joseph D'Ercole, Kathryn E. Saatman

Spinal Cord and Brain Injury Research Center Faculty Publications

Traumatic brain injury (TBI) survivors often suffer from long-lasting cognitive impairment that stems from hippocampal injury. Systemic administration of insulin-like growth factor-1 (IGF-1), a polypeptide growth factor known to play vital roles in neuronal survival, has been shown to attenuate posttraumatic cognitive and motor dysfunction. However, its neuroprotective effects in TBI have not been examined. To this end, moderate or severe contusion brain injury was induced in mice with conditional (postnatal) overexpression of IGF-1 using the controlled cortical impact (CCI) injury model. CCI brain injury produces robust reactive astrocytosis in regions of neuronal damage such as the hippocampus. We exploited …

Does It Have To Taste Bad To Be Good? Leveraging Pleasure To Enhance Learning, Karen H. Dougherty M.D.

Kentucky Journal of Higher Education Policy and Practice

Emerging information from the field of neuroscience has illuminated the workings of the brain’s pleasure circuit as a powerful motivator of human behavior. While much of the research has been driven by an effort to uncover the roots of addiction, an understanding of the aspects of pleasure can be applied to the design of teaching strategies to engage college students and improve their retention and persistence.

Factors Community College Faculty Consider Important To Academic Leadership, Juston C. Pate, Lance R. Angell

Kentucky Journal of Higher Education Policy and Practice

Although many of the pressures leaders face come from external sources, the expertise of the faculty should not be ignored when preparing community college leaders. The current study analyzed survey data from community college faculty across the state of Kentucky to determine which attributes they considered important to academic leadership. The faculty members who responded to an online survey regarded most highly factors pertaining to communication, character, decision-making, teamwork, work ethic, and personal relationships.

Development Of Novel In Vivo Chemical Probes To Address Cns Protein Kinase Involvement In Synaptic Dysfunction, D Martin Watterson, Valerie L. Grum-Tokars, Saktimayee M. Roy, James P. Schavocky, Brinda Desai Bradaric, Adam D. Bachstetter, Bin Xing, Edgardo Dimayuga, Faisal Saeed, Hong Zhang, Agnieszka Staniszewski, Jeffrey C. Pelletier, George Minasov, Wayne F. Anderson, Ottavio Arancio, Linda J. Van Eldik

Sanders-Brown Center on Aging Faculty Publications

Serine-threonine protein kinases are critical to CNS function, yet there is a dearth of highly selective, CNS-active kinase inhibitors for in vivo investigations. Further, prevailing assumptions raise concerns about whether single kinase inhibitors can show in vivo efficacy for CNS pathologies, and debates over viable approaches to the development of safe and efficacious kinase inhibitors are unsettled. It is critical, therefore, that these scientific challenges be addressed in order to test hypotheses about protein kinases in neuropathology progression and the potential for in vivo modulation of their catalytic activity. Identification of molecular targets whose in vivo modulation can attenuate synaptic …

Voluntary Exercise Protects Against Methamphetamine-Induced Oxidative Stress In Brain Microvasculature And Disruption Of The Blood--Brain Barrier, Michal Toborek, Melissa J. Seelbach, Cetewayo S. Rashid, Ibolya E. András, Lei Chen, Minseon Park, Karyn A. Esser

Physiology Faculty Publications

BACKGROUND: There is no effective therapeutic intervention developed targeting cerebrovascular toxicity of drugs of abuse, including methamphetamine (METH). We hypothesize that exercise protects against METH-induced disruption of the blood--brain barrier (BBB) by enhancing the antioxidant capacity of cerebral microvessels and modulating caveolae-associated signaling. Mice were subjected to voluntary wheel running for 5 weeks resembling the voluntary pattern of human exercise, followed by injection with METH (10 mg/kg). The frequency, duration, and intensity of each running session were monitored for each mouse via a direct data link to a computer and the running data are analyzed by Clock labTM Analysis software. …

Comparative Feedstock Analysis In Setaria Viridis L. As A Model For C4 Bioenergy Grasses And Panicoid Crop Species, Carloalberto Petti, Andrew Shearer, Mizuki Tateno, Mathew J. Ruwaya, Sue E. Nokes, Tom Brutnell, Seth Debolt

Horticulture Faculty Publications

Second generation feedstocks for bioethanol will likely include a sizable proportion of perennial C4 grasses, principally in the Panicoideae clade. The Panicoideae contain agronomically important annual grasses including Zea mays L. (maize), Sorghum bicolor (L.) Moench (sorghum), and Saccharum officinarum L. (sugar cane) as well as promising second generation perennial feedstocks including Miscanthus × giganteus and Panicum virgatum L. (switchgrass). The underlying complexity of these polyploid grass genomes is a major limitation for their direct manipulation and thus driving a need for rapidly cycling comparative model. Setaria viridis (green millet) is a rapid cycling C4 panicoid grass with a relatively …

Biomarkers For Ovarian Cancer, Eric T. Fung, Frederick Ueland, J. R. Van Nagell, Paul D. Depriest, Andre T. Baron

Epidemiology and Environmental Health Faculty Patents

The present invention provides protein-based biomarkers and biomarker combinations that are useful in qualifying ovarian cancer status in a patient. In particular, the biomarkers of this invention are useful to classify a subject sample as ovarian cancer, ovarian cancer of low malignant potential, benign ovarian disease or other malignant condition. The biomarkers can be detected by SELDI mass spectrometry.

Reversion Of The Arabidopsis Rpn12a-1 Exon-Trap Mutation By An Intragenic Suppressor That Weakens The Chimeric 5' Splice Site, Jasmina Kurepa, Yan Li, Jan A. Smalle

Plant and Soil Sciences Faculty Publications

BACKGROUND: In the Arabidopsis 26S proteasome mutant rpn12a-1, an exon-trap T-DNA is inserted 531 base pairs downstream of the RPN12a STOP codon. We have previously shown that this insertion activates a STOP codon-associated latent 5' splice site that competes with the polyadenylation signal during processing of the pre-mRNA. As a result of this dual input from splicing and polyadenylation in the rpn12a-1 mutant, two RPN12a transcripts are produced and they encode the wild-type RPN12a and a chimeric RPN12a-NPTII protein. Both proteins form complexes with other proteasome subunits leading to the formation of wild-type and mutant proteasome versions. The net result …

Qualitative Data Archiving In The Digital Age: Strategies For Data Preservation And Sharing, Lisa Cliggett

Anthropology Faculty Publications

Given the combination of recent mandates from funding agencies for data management plans and data sharing, and the explosion of data in digital form over the past two decades, it is time for the qualitative social science community to embrace digital archiving as an inherent component of research methodology. Archiving digital data ensures, at the least, that an individual scholar’s data is preserved and accessible to the user many decades into his or her career. Digital archiving also has the potential to preserve for the broader scholarly community, the full range of social science knowledge far beyond an individual researcher’s …

Influence Of Media Composition On The Growth Rate Of Chlorella Vulgaris And Scenedesmus Acutus Utilized For Co2 Mitigation, Czarena L. Crofcheck, Xinyi E, Aubrey Shea, Michael D. Montross, Mark Crocker, Rodney Andrews

Biosystems and Agricultural Engineering Faculty Publications

Atmospheric carbon dioxide levels have increased since the industrial revolution due to increasing combustion of fossil fuels. One possible CO₂ mitigation strategy is the use of microalgae for mitigation of CO₂. This paper focuses on the influence of media composition on the growth rate of two microalgae strains, Chlorella vulgaris and Scenedesmus actus. A KNO₃ based medium was found to work better for Chlorella, while a urea based equivalent worked better for Scenedesmus. The urea based media investigated here resulted in growth similar to that found with previously reported KNO₃ based media. …

Envelope Determinants Of Equine Lentiviral Vaccine Protection, Jodi K. Craigo, Corin Ezzelarab, Sheila J. Cook, Chong Liu, David W. Horohov, Charles J. Issel, Ronald C. Montelaro

Veterinary Science Faculty Publications

Lentiviral envelope (Env) antigenic variation and associated immune evasion present major obstacles to vaccine development. The concept that Env is a critical determinant for vaccine efficacy is well accepted, however defined correlates of protection associated with Env variation have yet to be determined. We reported an attenuated equine infectious anemia virus (EIAV) vaccine study that directly examined the effect of lentiviral Env sequence variation on vaccine efficacy. The study identified a significant, inverse, linear correlation between vaccine efficacy and increasing divergence of the challenge virus Env gp90 protein compared to the vaccine virus gp90. The report demonstrated approximately 100% protection …

A New Approach To Light-Weight Ablators Analysis: From Micro-Tomography Measurements To Statistical Analysis And Modeling, Nagi N. Mansour, Francesco Panerai, Alexandre Martin, Dilworth Y. Parkinson, Alastair A. Macdowell, Abdelmoula Haboub, Timothy A. Sandstrom, Tony Fast, Gerard L. Vignoles, Jean Lachaud

Mechanical Engineering Faculty Publications

The morphology characteristics and ablation behavior of a highly porous carbon fiber preform are studied using a combined experimental/numerical approach. Morphological characterization of the three-dimensional structure of the material is performed by hard X-rays synchrotron micro-tomography at the Advanced Light Source of Lawrence Berkeley National Laboratory. The resulting micro-tomography voxels are used to compute geometrical properties of the carbon preform, like porosity, specific surface area and tortuosity, that are otherwise indirectly measured through experimental techniques. The reconstructed volumes are used to build a computational grid for numerical simulations of the fibers' ablation. By modeling the diffusion of oxygen through the …

Volume Averaged Modeling Of The Oxidation Of Porous Carbon Fiber Material, Alexandre Martin

Mechanical Engineering Faculty Publications

Charring ablators remain the premium choice for space exploration missions that involve atmospheric re-entry. This type of ablative material is composed of a carbon matrix, usually made of fibers, which is then impregnated with a resin. During re-entry, the high heat flux produced by convective heating causes the material to chemically react. First, the resin pyrolyzes, and is vaporized into a gas that travels through the material, and is eventually ejected at the surface. Then, as the temperature rises, the surface of the porous matrix recess through ablative processes. For re-entry conditions typical of space exploration missions, this is mainly …

Multi-Dimensional Modeling Pyrolysis Gas Flow Inside Charring Ablators, Haoyue Weng, Alexandre Martin

Mechanical Engineering Faculty Publications

Using an ablative thermal/material response code, the importance of three-dimensionality for modeling ablative test-article is addressed. In particular, the simulation of the pyrolysis gas flow inside a porous material is presented, using two different geometries. The effects of allowing the gas to flow out of the side wall are especially highlighted. Results show that the flow inside the test-article is complex, and that the 0D or 1D assumption made in most Material Response (MR) codes might not be valid for certain geometries.

Library As Open Access Publisher: An Overview For Technical Service Librarians, Adrian K. Ho, Mary Beth Thomson

Library Presentations

There is a growing trend within libraries to provide publishing services in direct support of open access to scholarly communication. Technical service librarians have a long and rich history of acquiring, providing access to and preserving a variety of resources from publishers, societies, governments and individuals. This session will provide an overview of the activities, processes, rights issues, and systems involved in a library published or hosted open access journal. Adrian Ho, Director of Digital Scholarship, and Mary Beth Thomson, Associate Dean for Collections, Digital Scholarship, and Technical Services for the University of Kentucky Libraries will share their experiences with …