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Genetics, Genetic Testing, And The Specter Of Discrimination: A Discussion Using Hypothetical Cases, Richard H. Underwood, Ronald G. Cadle

Kentucky Law Journal

No abstract provided.

Cellular Basis Of Decreased Immune Responses To Pneumococcal Vaccines In Aged Mice, Manju Garg, Wei Luo, Alan M. Kaplan, Subbarao Bondada

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Previously, model systems were developed in our laboratory to study murine immune responses to the 23-valent pneumococcal polysaccharide vaccine Pnu-Imune, both in vivo and in vitro (M. Garg and B. Subbarao, Infect. Immun. 60:2329-2336, 1992; M. Garg, A. M. Kaplan, and S. Bondada, J. Immunol. 152: 1589-1596, 1994). Using these systems, we found that aged mice did not respond to the vaccine in vivo or in vitro. Cell separation studies showed that the unresponsiveness of the aged spleen cells to the vaccine was not due to an intrinsic B-cell defect or to T-cell-mediated immunosuppression but resulted from an accessory cell …

Patch Diseases In Kentucky Bluegrass Lawns, Paul C. Vincelli, A. J. Powell Jr.

Agriculture and Natural Resources Publications

“Patch diseases” can be very destructive when Kentucky bluegrass is grown under intensive management. Two patch diseases with similar symptoms can occur. Necrotic ring spot often appears in early summer. Summer patch, the more common disease in Kentucky landscapes, develops in middle to late summer.

These diseases are often a concern in lawns under intensive management for two reasons:

1. Improper application of certain lawn management practices, such as close mowing, can aggravate disease development.
2. When a lawn receives significant inputs of fertilizer and irrigation and frequent mowing, the homeowner or landscape manager usually has high aesthetic expectations and tolerates …

Guide For Control Of Annual And Perennial Flower And Ground Cover Diseases In The Landscape, John R. Hartman, Mary L. Witt

Agriculture and Natural Resources Publications

Herbaceous flowering plants are an asset to almost any landscape. Healthy plants are essential to maintaining a desirable aesthetic appearance. This publication suggests ways to control important infectious diseases of flowers and ground covers commonly grown in Kentucky.

Polyamine-Polyamine And Polyamine-Protein Transport Inhibitor Conjugates And Their Use As Pharmaceuticals And In Research Relating To Polyamine Transport, Shewan M. Aziz, Mark N. Gillespie

Pharmaceutical Sciences Faculty Patents

Novel classes of inhibitors which selectively inhibit the cellular transport of normally transported substances, specifically polyamines are taught which comprise (i) polymers of the transported substance or (ii) protein or polypeptide conjugates of the transported substance. These inhibitors may be used in vitro to assess the effect of the transported substance on cellular functions and in vivo for treating disease conditions involving transport of the particular substance, e.g., a polyamine.

A Survey Of Source And Finished Water Supplies For Giardia Cysts And Cryptosporidium Oocysts, Lisa Martin Sexton

KWRRI Research Reports

The occurrence of Giardia lamblia cysts and Cryptosporidium parvum oocysts was determined from source and treated drinking water supplies at selected water treatment facilities in Eastern Kentucky. Giardia and Cryptosporidium are two human protozoan parasites often found in water supplies due to fecal contamination. Characteristic cysts and oocysts were identified from large volume water samples, typically 100 gallons for raw samples and 1,000 gallons for finished water, using an indirect fluorescent antibody specific for Giardia and Cryptosporidium. Source water from four different water treatment facilities utilizing a reservoir, river, and two creeks were assayed. All sites exhibited Giardia lamblia …

Managing Alfalfa Diseases, Paul C. Vincelli

Kentucky Alfalfa and Stored Forage Conference

Diseases of alfalfa can limit productivity of this valuable forage crop. Alfalfa diseases can reduce forage yield, reduce forage quality, and decrease stand persistence. The effects of diseases can be dramatic, such as sudden stand loss due to Sclerotinia crown and stem rot. Often, the effects of alfalfa diseases are more subtle but are no less important. For example, alfalfa plants with Phytophthora root rot sometimes regrow slowly after cutting, resulting in a stunted stand showing no other obvious symptoms of disease.

Like most crop diseases, alfalfa diseases are most effectively managed by integrating as many control measures as practical. …

Enhanced Immunogenicity Of Protein-Dextran Conjugates: I. Rapid Stimulation Of Enhanced Antibody Responses To Poorly Immunogenic Molecules, Andrew Lees, Fred Finkelman, John K. Inman, Kim Witherspoon, Penny Johnson, Jasiri Kennedy, James J. Mond

Microbiology, Immunology, and Molecular Genetics Faculty Publications

In view of our observation that anti-immunoglobulin antibody conjugated to high-molecular-weight dextran stimulates high levels of B-cell activation (Brunswick et al. J. Immunol . 1989, 143, 1239), we coupled T cell-dependent antigens to dextran. When mice were immunized, in the absence of adjuvant, with a BSA-dextran conjugate (BSA-dex), a persistent, high-titre anti-BSA IgG1 response was induced. Titres were dose-dependent and seen with as little as 10 I~g of conjugated protein. Anti-BSA titres were detected as early as day 7, usually peaked at about day 14 and persisted for at least 4 weeks. Anti-hapten antibodies were also elicited in mice that …

Reversal Of Age-Associated Decline In Immune Response To Pnu-Imune Vaccine By Supplementation With The Steroid Hormone Dehydroepiandrosterone, Manju Garg, Subbarao Bondada

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Recently, we reported that murine antibody responses to the 23-valent pneumococcal polysaccharide (Pnu-Imune) vaccine declined with age. Here we present data to support the concept that age-associated immune defects are not only due to intrinsic defects in immune cells but are also due to extrinsic factors emanating from the neuroendocrine system. We found that supplementation with dehydroepiandrosterone, a steroid hormone known to be reduced in the aged, corrects the immune deficiency of aged mice and significantly enhanced their splenic immune responses to the Pnu-Imune vaccine.

Immune Responses Of Systemic And Mucosal Lymphoid Organs To Pnu-Imune Vaccine As A Function Of Age And The Efficacy Of Monophosphoryl Lipid A As An Adjuvant, Manju Garg, Subbarao Bondada

Microbiology, Immunology, and Molecular Genetics Faculty Publications

A murine model system was established to study immune responses to the Pnu-Imune vaccine, which is made up of 23 different pneumococcal capsular polysaccharides. In this animal model, antibody-forming cell responses to 21 of 23 individual polysaccharides in the vaccine were detected. The Pnu-Imune vaccine elicited good antibody responses from the spleens and mesenteric lymph nodes (MLN) of young mice, whereas a variety of other peripheral lymph nodes were unresponsive. The immunoglobulin M plaque-forming cell (PFC) response in the spleen to the Pnu-Imune vaccine (given intraperitoneally or subcutaneously) decreased dramatically with increasing age. However, the spleen and MLN differed in …

Commonwealth V. Wasson: Invalidating Kentucky's Sodomy Statute, Thomas P. Lewis

Kentucky Law Journal

No abstract provided.

Part Of The Moving Stream: State Constitutional Law, Sodomy, And Beyond, Shirley A. Wiegand, Sara Farr

Kentucky Law Journal

No abstract provided.

Alfalfa Disease Management, Paul C. Vincelli

Kentucky Alfalfa and Stored Forage Conference

Alfalfa can be a vigorous and productive forage crop for Kentucky farmers. Like all farm crops, however, alfalfa is subject to infectious diseases that can limit forage production. Managing these diseases is an important part of economical alfalfa production.

Primary Antibody Responses To Thymus-Independent Antigens In The Lungs And Hilar Lymph Nodes Of Mice, S. Niranjan Goud, Alan M. Kaplan, Subbarao Bondada

Sanders-Brown Center on Aging Faculty Publications

B lymphocytes from the pulmonary lymphoid tissues were stimulated with a variety of thymus-independent (TI) antigens by intratracheal (i.t.) immunization. Immune responses in the lungs and hilar lymph nodes (HLN), which are part of the localized lymphoid tissue, as well as in the spleen, the systemic lymphoid organ, were studied. Thus, primary i.t. immunization of mice with the TI-1 antigen trinitrophenyl-lipopolysaccharide (TNP-LPS) elicited both antigen-specific and polyclonal plaque-forming cell responses from HLN, lung, and splenic B lymphocytes. These responses appeared as early as 3 days after immunization and declined by day 7. Similar immunization with another TI-1 antigen, TNP-Brucella …

Managing Diseases Of Alfalfa, Paul C. Vincelli, Garry D. Lacefield, Jimmy C. Henning

Agriculture and Natural Resources Publications

Alfalfa can be a vigorous and productive forage crop for Kentucky farmers. Like all farm crops, however, alfalfa is subject to infectious diseases that can limit forage production. Managing these diseases is an important part of economical alfalfa production.

Alfalfa diseases can cause reduced forage yield, reduced forage quality, and decreased stand persistence. Sometimes, the effects of infectious diseases can be dramatic, such as sudden stand loss in a fall-seeded crop caused by Sclerotinia crown and stem rot. Often, the effects of diseases on alfalfa are more subtle but no less important. For example, alfalfa plants with Phytophthora root rot …

Disease Management In The Home Lawn, Paul C. Vincelli, A. J. Powell Jr.

Agriculture and Natural Resources Publications

Numerous infectious diseases can occur on lawns in Kentucky. Unless diagnosed and managed, these diseases can sometimes cause extensive damage. A sound disease management program benefits your lawn in two ways:

• reduces the severity of lawn diseases
• improves the lawn's recovery should a disease outbreak occur.

This publication describes lawn management practices that can help control diseases of turfgrasses commonly used in home lawns -- Kentucky bluegrass, tall fescue, and perennial ryegrass. You can control diseases of turfgrasses most effectively by using as many of the following lawn management practices as feasible.

The first step in improving your lawn is …

The Frontier Nursing Service Oral History Project: An Annotated Guide, Susan E. Allen, Terry L. Birdwhistell

Library Occasional Papers Series

No abstract provided.

Kentucky Tort Law: Defamation And The Right Of Privacy By David A. Elder, Richard C. Ausness

Kentucky Law Journal

No abstract provided.

Kentucky Tort Law: Defamation And The Right Of Privacy, Richard C. Ausness

Law Faculty Scholarly Articles

This book review examines Kentucky Tort Law: Defamation and the Right of Privacy by David A. Elder, which was published in 1983.

Role Of A Nonimmunoglobulin Cell Surface Determinant In The Activation Of B Lymphocytes By Thymus-Independent Antigens*, Subbarao Bondada, Donald E. Mosier, Aftab Ahmed, James J. Mond, Irwin Scher, William E. Paul

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Lyb 5 is a B-cell alloantigen which is expressed on 50-60% of B cells. It was defined originally on the basis of cytotoxicity. We have described a new reactivity within the anti-Lyb 5 serum on the basis of selective inhibition of antibody responses in vitro by this antiserum in the absence of complement. This inhibitory activity of anti-Lyb 5.1 serum appears to be due to recognition of antigenic determinants different from the prototype antigens detected in the cytotoxicity assay. Anti-Lyb 5 serum incorporated into spleen cell cultures selectively inhibits antibody responses to a class of thymus-independent antigens (TI-2) previously characterized …

Tritium (3h) Radiolabeling Of Protein A And Antibody To High Specific Activity: Application To Cell Surface Antigen Radioimmunoassays, Ronald L. Wilder, Catherine C. Yuen, Subbarao Bondada, Virgil L. Woods, Cornelius B. Alexander, Rose G. Mage

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Staphylococcal protein A and several different immunoglobulins have been radio-labeled to high specific activities (> 106 cpm/pg) by reductive methylation with tritiated (3H) sodium borohydride. The proteins retain excellent functional and antigenic properties. The utility of these reagents in a variety of assays for cell surface antigens is illustrated. The results indicate that this radiolabeling procedure may become the method of choice for many cell surface and solution immunoassays.

Libel Per Quod In Florida, Richard C. Ausness

Law Faculty Scholarly Articles

The purpose of this article is to trace the development of the rules of defamation with particular reference to extrinsic fact. A defamatory communication is one that tends to diminish the esteem, respect, good will, or confidence in which a person is held or to excite adverse, derogatory, or unpleasant feelings or opinions against him. To be actionable under the modem law, however, the defendant's statement must be capable of a defamatory meaning in the sense normally understood.

Defamation consists of the separate torts of libel and slander. Historically, these torts evolved independently of each other, and as a result …

Constitutional Protection For Freedom Of Movement: A Time For Decision, Sheldon Elliot Steinbach

Kentucky Law Journal

No abstract provided.

Appendicitis In Law, Kurt Garve

Kentucky Law Journal

No abstract provided.

Is Compulsory Human Sterilization The Long Sought Solution For The Problem Of Our Mental Incompetents?, Robert Edwin Hatton Jr.

Kentucky Law Journal

No abstract provided.

Compensable Aggravation And Acceleration Of Pre-Existing Infirmities Under Workmen's Compensation Act, Kurt Garve

Kentucky Law Journal

No abstract provided.

Case Comments, Kentucky Law Journal

Kentucky Law Journal

No abstract provided.

Case Comments, Kentucky Law Journal

Kentucky Law Journal

No abstract provided.

Case Comments, Kentucky Law Journal

Kentucky Law Journal

No abstract provided.

Case Comments, Kentucky Law Journal

Kentucky Law Journal

No abstract provided.