Digital Commons Network^™

Experiences Of Eight Ohio Beef And Sheep Producers With Year Round Grazing, H M. Bartholomew, S L. Boyles, B Carter, E Vollborn, D Miller, R M. Sulc

IGC Proceedings (1977-2023)

Livestock producers participated in a demonstration project to extend the grazing season and evaluate dormant forage quality and yield. Differences were not detected in yields among forage species but yield differences existed among sites. Yield estimates did not change over time across all forage species but some sites were only able to participate for two months. Producers utilizing fescue beyond November had reductions in herbage mass. Crude protein, available crude protein, acid detergent fiber, and neutral detergent fiber content differed among forage species and sites. Energy and lignin content were similar for all forage species and sites. Sodium and copper …

Implementation, Enrollment, And Engagement In An Opt-Out Telehealth Pharmacist-Assisted Tobacco Treatment Program For Patients Seen In Oncology Outpatient Clinics, K. Michael Cummings, Benjamin A. Toll, Vincent Talbot, Avery Roberson, Dianne Wilson, Martha Dunlap, Emily C. Ware, Amanda M. Palmer, Asia A. Bliss, Vincent S. Anokye, Graham Warren

Markey Cancer Center Faculty Publications

OBJECTIVE: To describe the workflow, reach, cost, and self-reported quit rates for an opt-out tobacco treatment program (TTP) for patients seen in 43 oncology outpatient clinics.

METHODS: Between May 25, 2021, and December 31, 2022, adult patients (≥18 years) visiting clinics affiliated with the Medical University of South Carolina Hollings Cancer Center were screened for smoking status. Those currently smoking were referred to a telehealth pharmacy-assisted TTP. An attempt was made to contact referred patients by phone. Patients reached were offered free smoking cessation counseling and a 2-week starter kit of nicotine replacement medication. A random sample of 420 patients …

Iron Oxide Nanozymes Enhanced By Ascorbic Acid For Macrophage-Based Cancer Therapy, Zhongchao Yi, Xiaoyue Yang, Ying Liang, Sheng Tong

Markey Cancer Center Faculty Publications

In recent years, using pharmacological ascorbic acid has emerged as a promising therapeutic approach in cancer treatment, owing to its capacity to induce extracellular hydrogen peroxide (H2O 2) production in solid tumors. The H2 O 2 is then converted into cytotoxic hydroxyl free radicals (HO•) by redox-active Fe2+ inside cells. However, the high dosage of ascorbic acid required for efficacy is hampered by adverse effects such as kidney stone formation. In a recent study, we demonstrated the efficient catalytic conver- sion of H2 O2 to HO• by wüstite (Fe 1−xO) nanoparticles (WNPs) through a heterogenous Fenton reaction. Here, we explore …

Challenges Of Spatially Resolved Metabolism In Cancer Research, Andrew N. Lane, Richard M. Higashi, Teresa W-M Fan

Markey Cancer Center Faculty Publications

Stable isotope-resolved metabolomics comprises a critical set of technologies that can be applied to a wide variety of systems, from isolated cells to whole organisms, to define metabolic pathway usage and responses to perturbations such as drugs or mutations, as well as providing the basis for flux analysis. As the diversity of stable isotope-enriched compounds is very high, and with newer approaches to multiplexing, the coverage of metabolism is now very extensive. However, as the complexity of the model increases, including more kinds of interacting cell types and interorgan communication, the analytical complexity also increases. Further, as studies move further …

Native Grasslands Of Yugoslavia As A Potential For "Biologically High Quality Food" Production - To Be Preserved And Used For The Benefit Of All, Djordjevic-Milosevic Suzana, Negovanovic Dragan, Zakonovic Miodrag

IGC Proceedings (1977-2023)

Natural grasslands are the base of the livestock production in the hilly-mountainous regions of Yugoslavia. Poor economic power of the owners of these areas brought about a complete dying out of agricultural production in these regions in the last few years. In the same time, the lack of financial resources made it impossible for the farmers to use the fertilisers, protective and other chemical agents which result in a high degree of environmental preservation. Due to current economic policy at this time the population has moved out and there is no industrialisation. The revival of agriculture on the hilly-mountainous regions …

Beef Production From Grazed Grass And Grass/Clover Swards, Edward G. O'Riordan

IGC Proceedings (1977-2023)

grazed grass (fertiliser nitrogen treated) and grass/clover swards, during two grazing seasons which extended from early-April until mid-November. Both permanent (Treatments A, B and C) and reseeded (Treatment D, E and F) swards were respectively stocked with 3000, 2500 and 2000 kg liveweight/ha in Spring using fifteen steers/treatment (liveweight = 570kg/head). There were small and generally non-significant treatment effects on final liveweight in both years, but heavier carcasses were observed on the reseeded swards (P<0.05). Clover based swards (Treatments B, C and E, F) produced heavier carcasses (P<0.05) than the nitrogen fertilised grass swards (Treatments A and D). Pasture production ranged from 7540 to 10,469 and from 6360 to 11,606 kg DM/ha in year 1 and 2 respectively, with highest yields being observed on the nitrogen fertilised treatment and on the reseeded swards.

Zea Mays Grazing In Cattle Production Systems, V Mundy, B E. Anderson, T L. Gompert, T L. Mader, C A. Shapiro

IGC Proceedings (1977-2023)

Nebraska producers have used silage maize (Zea mays L.) hybrids as a grazed forage in dairy and beef production systems. In an onfarm case study, farmers and researchers collaborated to gather information on production practices, grazing management, and plant characteristics of grazing maize within three forage-based dairy and beef systems. Production practices were generally similar to conventional grain corn production, except that in one system maize was seeded directly into a suppressed grass sod. Milk production from grazing maize was 23 kg/day/animal, compared to 24 kg/day/ animal before using maize and 22 kg/day/animal after using maize. Average gain of …

Community-Level Factors And Their Associations With Changing Opioid Overdose Fatality Rates In Kentucky, 2019–2021, Shawn R. Nigam, Philip M. Westgate, Svetla Slavova, Rachel Vickers-Smith, Katherine L. Thompson

Journal of Appalachian Health

Introduction: Kentucky has one of the highest opioid overdose fatality rates in the United States, which has increased significantly from 2019 to 2020. The COVID-19 pandemic has caused lasting effects on mental health and health care, which have been linked with increased opioid overdose. These effects are exacerbated in Appalachian regions, where there is a lack of sufficient access to community pharmacies and adequate health care.

Purpose: In this study, we characterize changes in opioid overdose fatality rates in Kentucky from 2019 to 2021, with a specific focus on changes in Appalachian vs non-Appalachian counties. We aim to identify associations …

Mechanisms And Mass Transfer Kinetics Of Cr(Vi) Adsorbed By Mussel Shell Incorporated Adsorptive Membrane, Mohamad Ali Fulazzaky, Nur Atikah Abdul Salim, Mohd Hafiz Puteh, Tiffany L. Messer, Mohd Hafiz Dzarfan Othman, Juhana Jaafar, Ahmad Fauzi Ismail, Mukhlis A. Rahman

UK CARES Faculty Publications

The presence of Cr(VI) results in pollution that threatens human health and aquatic life. Therefore, there is an urgent need for Cr(VI) to be removed from contaminated water. The mussel shell incorporated polyethersulfone (MSPES) membranes designed to form the flat sheet adsorptive membrane (FSAM) crossflow treatment systems was used to remove Cr(VI) from synthetic solution. The physicochemical properties of the MSPES membrane were verified using an atomic force microscope, scanning electron microscope, and water contact angle goniometer. The performance of MSPES2.0 to remove Cr(VI) ions ranged from 15.00 to 16.05 % was higher than that of MSPES2.2 ranged from 10.00 …

Forage News [2024-07], University Of Kentucky

Forage News

No abstract provided.

Protease Expression In The Human And Rat Cumulus–Oocyte Complex During The Periovulatory Period: A Role In Cumulus–Oocyte Complex Migration, Ketan Shrestha, Muraly Puttabyatappa, Michelle Arelia Ann Wynn, Patrick R. Hannon, Linah Al-Alem, Katherine L. Rosewell, James Akin, Thomas E. Curry

UK CARES Faculty Publications

The migratory and matrix-invading capacities of the cumulus–oocyte complex have been shown to be important for the ovulatory process. In metastatic cancers, these capacities are due to increased expression of proteases, however, there is limited information on protease expression in the cumulus–oocyte complexes. The present study examined cumulus–oocyte complex expression of plasmins, matrix metalloproteases, and A Disintegrin and Metalloproteinase with Thrombospondin Motifs family members in the rat and human. In the rat, human chorionic gonadotropin (hCG) administration increased cumulus–oocyte complex expression of Mmp2, Mmp9, Mmp13, Mmp14, Mmp16, Adamts1, and the protease inhibitors Timp1, Timp3, and Serpine1 by 8–12 h. This …

Mapping Recent Timber Harvest Activity In A Temperate Forest Using Single Date Airborne Lidar Surveys And Machine Learning: Lessons For Conservation Planning, G. Bursh Fisher, Andrew J. Elmore, Matthew C. Fitzpatrick, Darin J. Mcneil, Jeff W. Atkins, Jeffery L. Larkin

Earth and Environmental Sciences Faculty Publications

Effective planning for natural resource management and wildlife conservation requires detailed information on vegetation structure at landscape scales and how structure is influenced by land- use practices. In many forested landscapes, the largest impacts of land use on forest structure are driven by forest management activities, which can include invasive species control, prescribed fire, partial harvests (e.g. shelterwood harvests) or overstory removals and clearcuts. Active timber management is often used to achieve forest conservation objectives, but to be used effectively, managers require knowledge of harvest frequency and extent in adjacent forests and at landscape scales. In this paper, we develop …

Performance And Environmental Assessment Of Biochar-Based Membranes Synthesized From Traditional And Eco-Friendly Solvents, Abelline Katusiime Fionah, Isaac Oluk, Laura Brady, Diana M. Byrne, Isabel Escobar

UK CARES Faculty Publications

Water contamination resulting from coal spills is one of the largest environmental problems affecting communities in the Appalachia Region of the United States. This coal slurry contains potentially toxic substances, such as hydrocarbons, heavy metals, and coal cleaning chemicals, and its leakage into water bodies (lakes, rivers, and aquifers) can lead to adverse health effects not only for freshwater bodies and plant life but also for humans. This study focused on two major experiments. The first experiment involved the use of biochar to create a biochar–polysulfone (BC-PSf) flat-sheet multifunctional membrane to remove organic contaminants, and the other major experiment compared …

Examining The Progress In Additive Manufacturing In Supporting Lean, Green And Sustainable Manufacturing: A Systematic Review, Catherine Maware, Rumbidzai Muvunzi, Tinotenda Machingura, Ilesanmi Daniyan

Institute for Sustainable Manufacturing Faculty Publications

The quest for waste reduction and the development of manufacturing processes that meet the economic, social and environmental requirements necessitate this study. Additive manufacturing is an emerging digital technology that can be used to seamlessly develop a product through material deposition in layers. The study aims to investigate the progress made in the development of additive manufacturing to support lean, green and sustainable manufacturing. The study employs a systematic literature review approach, specifically the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA). A total of 158 articles identified from different academic databases that detailed empirical, conceptual and theoretical findings …

A Comprehensive Study Of Library-Led Textbook Affordability Initiatives In The United States, Mitchell Scott, Rachel E. Scott

Library Faculty and Staff Publications

This study presents findings from a survey and interviews investigating library-led textbook affordability initiatives in the United States. The results document diverse considerations and divergences in workflows, challenges librarians face in establishing and maintaining textbook affordability programs, and the intersection of these initiatives with library and institutional strategies. Findings suggest that these programs have grown in number and scale over the past few years, accelerated by the COVID-19 pandemic lockdowns, and are sustained—even without permanent, designated funding—due to consistently positive perceptions about their impact on student success, just-in-time delivery, and alignment with library and institutional goals.

University Of Kentucky Libraries' Libqual+ Report 2024, Julene L. Jones

Library Faculty and Staff Publications

Report of University of Kentucky's 2024 LibQUAL+ results

Developing A Cross-Asset Allocation Mechanism, Jon Wilcoxson, Bryan Gibson, Bethany Paris

Kentucky Transportation Center Research Report

The Kentucky Transportation Cabinet (KYTC) has developed several tools and methods to compare the value of proposed projects within specific programs and asset classes, including the Enhanced Bridge Prioritization Index, Enhanced Pavement Prioritization Index, and the Strategic Highway Investment Formula for Tomorrow (SHIFT). Although immensely valuable, these methods and tools do not offer a convenient way of comparing the contributions of individual projects to meeting overall KYTC performance goals. As such, the Cabinet needs a robust mechanism for comparing the impact of projects across its capital, safety, and asset management programs. Following a review of cross-asset allocation frameworks described in …

Decentralized Clinical Trials For Medications To Reduce The Risk Of Dementia: Consensus Report And Guidance, Leanne Howard, Carla Abdelnour, Erin Abner, Ricardo F. Allegri, Hiroko H. Dodge, Serge Gauthier, Camilla M. Hoyos, Gregory Jicha, Patrick G. Kehoe, Catherine J. Mummery, Adesola Ogunniyi, Nikolaos Scarmeas, Xiaoying Chen, Jodie R. Titiner, Christopher R. Weber, Ruth Peters

Neurology Faculty Publications

INTRODUCTION: Recent growth in the functionality and use of technology has prompted an increased interest in the potential for remote or decentralized clinical trials in dementia. There are many potential benefits associated with decentralized medication trials, but we currently lack specific recommendations for their delivery in the dementia field.

METHODS: A modified Delphi method engaged an expert panel to develop recommendations for the conduct of decentralized medication trials in dementia prevention. A working group of researchers and clinicians with expertise in dementia trials further refined the recommendations.

RESULTS: Overall, the recommendations support the delivery of decentralized trials in dementia prevention …

Type-2 Diabetes, Pancreatic Amylin, And Neuronal Metabolic Remodeling In Alzheimer's Disease, Noah Leibold, James R. Bain, Florin Despa

Neurology Faculty Publications

Type-2 diabetes raises the risk for Alzheimer’s disease (AD)-type dementia and the conversion from mild cognitive impairment to dementia, yet mechanisms connecting type-2 diabetes to AD remain largely unknown. Amylin, a pancreatic 𝜷-cell hormone co-secreted with insulin, participates in the central regulation of satiation, but also forms pancreatic amyloid in persons with type-2 diabetes and synergistically interacts with brain amyloid 𝜷 (A𝜷) pathology, in both sporadic and familial Alzheimer’s disease (AD). Growing evidence from studies of tumor growth, together with early observations in skeletal muscle, indicates amylin as a potential trigger of cellular metabolic reprogramming. Because the blood, cerebrospinal fluid, …

A Mini-Review Of Strategies For Recruiting Older African Americans To Alzheimer's Disease Research., Darlingtina Esiaka, Christina C Yarborough, Bernadette A Fausto, Mark A Gluck

Behavioral Science Faculty Publications

African Americans experience higher incidence and prevalence of Alzheimer's disease (AD). Yet, they continue to be underrepresented in AD research, limiting the ability to generalize findings to the increasingly diverse US population. To reduce AD disparities, targeted efforts are needed to increase the representation of African Americans in AD research. This mini review identified evidence-based strategies that increased research participation among older African Americans. Four recruitment strategies emerged from eight published peer-reviewed studies that directly evaluated the effectiveness of strategies aimed at increasing the number of African American participants in Alzheimer's research. The strategies include community outreach and education, face-to-face …

Extended-Release 7-Day Injectable Buprenorphine For Patients With Minimal To Mild Opioid Withdrawal., Gail D'Onofrio, Andrew A. Herring, Jeanmarie Perrone, Kathryn Hawk, Elizabeth A. Samuels, Ethan Cowan, Erik Anderson, Ryan Mccormack, Kristen Huntley, Patricia Owens, Shara Martel, Mark Schactman, Michelle R. Lofwall, Sharon L. Walsh, James Dziura, David A. Fiellin

Behavioral Science Faculty Publications

IMPORTANCE: Buprenorphine is an effective yet underused treatment for opioid use disorder (OUD).

OBJECTIVE: To evaluate the feasibility (acceptability, tolerability, and safety) of 7-day injectable extended-release buprenorphine in patients with minimal to mild opioid withdrawal.

DESIGN, SETTING, AND PARTICIPANTS: This nonrandomized trial comprising 4 emergency departments in the Northeast, mid-Atlantic, and Pacific geographic areas of the US included adults aged 18 years or older with moderate to severe OUD and Clinical Opiate Withdrawal Scale (COWS) scores less than 8 (minimal to mild), in which scores range from 0 to 7, with higher scores indicating increasing withdrawal. Exclusion criteria included methadone-positive …

A Pathologic Study Of Perivascular Ptdp-43 Lin Bodies In Late-Nc, Ryan K. Shahidehpour, Peter T. Nelson, Adam D. Bachstetter

Neurology Faculty Publications

Background TAR DNA-Binding Protein 43 (TDP-43) pathological inclusions are a distinctive feature in dozens of neurodegenerative pathologies, including limbic-predominant age-related TDP-43 encephalopathy neuropathologic change (LATE-NC). Prior investigations identified vascular-associated TDP-43-positive micro-lesions, known as “Lin bodies,” located on or near the brain capillaries of some individuals with LATE-NC. This study aimed to investigate the relationship between the accumulation of Lin bodies and glial cells in LATE-NC and the potential co-localization with ferritin, a protein associated with iron storage. Using multiplexed immunohistochemistry and digital pathology tools, we conducted pathological analyses to investigate the relationship between Lin bodies and glial markers (GFAP for …

Applying Machine Learning Models Derived From Administrative Claims Data To Predict Medication Nonadherence In Patients Self-Administering Biologic Medications For Inflammatory Bowel Disease, Christian Rhudy, Courtney Perry, Michael Wesley, David Fardo, Cody Bumgardner, Syed Hassan, Terrence Barrett, Jeffery Talbert

Biostatistics Faculty Publications

BACKGROUND: Adherence to self-administered biologic therapies is important to induce remission and prevent adverse clinical outcomes in Inflammatory bowel disease (IBD). This study aimed to use administrative claims data and machine learning methods to predict nonadherence in an academic medical center test population.

METHODS: A model-training dataset of beneficiaries with IBD and the first unique dispense of a self-administered biologic between June 30, 2016 and June 30, 2019 was extracted from the Commercial Claims and Encounters and Medicare Supplemental Administrative Claims Database. Known correlates of medication nonadherence were identified in the dataset. Nonadherence to biologic therapies was defined as a …

Β-Aminopropionitrile Induces Distinct Pathologies In The Ascending And Descending Thoracic Aortic Regions Of Mice, Michael K. Franklin, Hisashi Sawada, Sohei Ito, Deborah A. Howatt, Naofumi Amioka, Ching Ling Liang, Nancy Zhang, David B. Graf, Jessica J. Moorleghen, Yuriko Katsumata, Hong S. Lu, Alan Daugherty

Biostatistics Faculty Publications

BACKGROUND: β-aminopropionitrile (BAPN) is a pharmacological inhibitor of LOX (lysyl oxidase) and LOXLs (LOX-like proteins). Administration of BAPN promotes aortopathies, although there is a paucity of data on experimental conditions to generate pathology. The objective of this study was to define experimental parameters and determine whether equivalent or variable aortopathies were generated throughout the aortic tree during BAPN administration in mice. METHODS: BAPN was administered in drinking water for a period ranging from 1 to 12 weeks. The impacts of BAPN were first assessed with regard to BAPN dose, and mouse strain, age, and sex. BAPN-induced aortic pathological characterization was …

Dual Light Emission Of Cssni3-Based Powders Synthesized Via A Mechanochemical Process, Xuan Huang, Xiaobing Tang, Xiyu Wen, Yuebin Charles Lu, Fuqian Yang

Chemical and Materials Engineering Faculty Publications

Lead toxicity has hindered the wide applications of lead halide perovskites in optoelec- tronics and bioimaging. A significant amount of effort has been made to synthesize lead-free halide perovskites as alternatives to lead halide perovskites. In this work, we demonstrate the feasibility of synthesizing CsSnI3-based powders mechanochemically with dual light emissions under ambi- ent conditions from CsI and SnI2 powders. The formed CsSnI3-based powders are divided into CsSnI3-dominated powders and CsSnI3-contained powders. Under the excitation of ultraviolet light of 365 nm in wavelength, the CsSnI3-dominated powders emit green light with a wavelength centered at 540 nm, and the CsSnI3-contained powders …

Ceramide-Mediated Orchestration Of Oxidative Stress Response Through Filopodia-Derived Small Extracellular Vesicles, Zainuddin Quadri, Ahmed Elsherbini, Simone M. Crivelli, Salim S. El-Amouri, Priyanka Tripathi, Zhihui Zhu, Xiaojia Ren, Liping Zhang, Stefka D. Spassieva, Mariana N. Nikolova‑Karakashian, Erhard Bieberich

Markey Cancer Center Faculty Publications

Extracellular vesicles (EVs) are shed from the plasma membrane, but the regulation and function of these EVs remain unclear. We found that oxidative stress induced by H 2 O 2 in Hela cells stimulated filopodia formation and the secretion of EVs. EVs were small (150 nm) and labeled for CD44, indicating that they were derived from filopodia. Filopodia-derived small EVs (sEVs) were enriched with the sphingolipid ceramide, consistent with increased ceramide in the plasma membrane of filopo- dia. Ceramide was colocalized with neutral sphingomyelinase 2 (nSMase2) and acid sphingomyelinase (ASM), two sphingomyelinases generating ceramide at the plasma membrane. Inhibition of …

Soxb1 Transcription Factors Are Essential For Initiating And Maintaining Neural Plate Border Gene Expression, Elizabeth Schock, Joshua R. York, Austin P. Li, Ashlyn Y. Tu, Carole Labonne

Markey Cancer Center Faculty Publications

SoxB1 transcription factors (Sox2/3) are well known for their role in early neural fate specification in the embryo, but little is known about functional roles for SoxB1 factors in non-neural ectodermal cell types, such as the neural plate border (NPB). Using Xenopus laevis, we set out to determine whether SoxB1 transcription factors have a regulatory function in NPB formation. Here, we show that SoxB1 factors are necessary for NPB formation, and that prolonged SoxB1 factor activity blocks the transition from a NPB to a neural crest state. Using ChIP-seq, we demonstrate that Sox3 is enriched upstream of NPB genes in …

14-3-3Ζ Suppresses Rankl Signaling By Destabilizing Traf6, R. Ayyasamy, S. Fan, P. Czernik, B. Lecka-Czernik, Somsubhra Chattopadhyay, R. Chakravarti

Markey Cancer Center Faculty Publications

Macrophages are essential regulators of inflammation and bone loss. Receptor activator of nuclear factor-κβ ligand (RANKL), a pro-inflammatory cytokine, is responsible for macrophage differentiation to osteoclasts and bone loss. We recently showed that 14-3-3ζ-knockout (Ywhaz^KO) rats exhibit increased bone loss in the inflammatory arthritis model. 14-3-3ζ is a cytosolic adaptor protein that actively participates in many signaling transductions. However, the role of 14-3-3ζ in RANKL signaling or bone remodeling is unknown. We investigated how 14-3-3ζ affects osteoclast activity by evaluating its role in RANKL signaling. We utilized 14-3-3ζ-deficient primary bone marrow–derived macrophages obtained from wildtype and Ywhaz^{KO …}

Discussions Of Cancer Survivorship Care Needs: Are There Rural Versus Urban Inequities?, Tyrone F. Borders, Lindsey R. Hammerslag

Markey Cancer Center Faculty Publications

Background: Rural cancer survivors may face greater challenges receiving survivorship care than urban cancer survivors.

Purpose: To test for rural versus urban inequities and identify other correlates of discussions about cancer survivorship care with healthcare professionals.

Methods: Data are from the 2017 Medical Expenditure Panel Survey (MEPS), which included a cancer survivorship supplement. Adult survivors were asked if they discussed with a healthcare professional 5 components of survivorship care: need for follow-up services, lifestyle/health recommendations, emotional/social needs, long-term side effects, and a summary of treatments received. The Behavioral Model of Health Services guided the inclusion of predisposing, enabling, and need …

Diffuse Reflectance Spectroscopy For Optical Characterizations Of Orthotopic Head And Neck Cancer Models In Vivo, Pranto Soumik Saha, Jing Yan, Caigang Zhu

Markey Cancer Center Faculty Publications

We demonstrated an easy-to-build, portable diffuse reflectance spectroscopy device along with a Monte Carlo inverse model to quantify tissue absorption and scattering-based parameters of orthotopic head and neck cancer models in vivo. Both tissue-mimicking phantom studies and animal studies were conducted to verify the optical spectroscopy system and Monte Carlo inverse model for the accurate extraction of tissue optical properties. For the first time, we reported the tissue absorption and scattering coefficients of mouse normal tongue tissues and tongue tumor tissues. Our in vivo animal studies showed reduced total hemoglobin concentration, lower tissue vascular oxygen saturation, and increased tissue scattering …