Digital Commons Network^™

Linkage Facilitation For Opioid Use Disorder In Criminal Legal System Contexts: A Primer For Researchers, Clinicians, And Legal Practitioners, Milan F. Satcher, Steven Belenko, Anthony Coetzer-Liversage, Khirsten J. Wilson, Michael R. Mccart, Tess K. Drazdowski, Amanda Fallin-Bennett, Nickolas Zaller, Alysse M. Schultheis, Aaron Hogue, Noel Vest, Ashli J. Sheidow, Brandon Del Pozo, Dennis P. Watson, Patrick F. Hibbard, Randy Stevens, L. A. R. Stein

Nursing Faculty Publications

At the intersection of drug policy, the opioid crisis, and fragmented care systems, persons with opioid use disorder (OUD) in the United States are significantly vulnerable to contact with the criminal legal system (CLS). In CLS settings, provision of evidence-based treatment for OUD is variable and often secondary to punitive approaches. Linkage facilitation at every touch point along the CLS Sequential Intercept Model has potential to redirect persons with OUD into recovery-oriented systems of care, increase evidence-based OUD treatment connections, and therefore reduce CLS re-exposure risk. Research in this area is still nascent. Thus, this narrative review explores the state …

Evaluating The Effectiveness Of A Student-Run Clinic On Reducing Cigarette Smoking In A Homeless Population, Briana Taormina, Bryson Jones, Kiah Gledhill Md, Audrey Darville Phd, Aprn, Cpaha-Tt

ePublications of Emerging Medical Research Scholars

Objective

Cigarette smoking remains a major health crisis in the United States, claiming the lives of hundreds of thousands of tobacco users each year. Access to evidence-based smoking cessation remains a challenge for many people that use tobacco products. Prior research demonstrates that the homeless population is exceedingly vulnerable to cigarette smoking and faces more barriers to quitting than their domiciled counterparts. This study explores the effectiveness of a free Smoking Cessation Clinic (SCC) within a homeless shelter for women and children, run by a nurse practitioner and a clinical nurse specialist trained as Tobacco Treatment Specialists, along with two …

Pasture Plant Identification In The Western Queensland Arid Zone, J A. Milson, I F. Beale, J A. Reynolds, J J. Bushwell, D Phelps

IGC Proceedings (1977-2023)

Since plant species composition is an important indicator of the health or condition of pastures and therefore the land, a first step for graziers interested in conserving the total range resource is to understand vegetation species and thus be able to identify them and to recognise species changes. This project is aimed at producing and distributing colour leaflets of the major native and problem plant species (including woody weeds) in the four major community types in western Queensland. The number of colour leaflels for the series totals 60. The number of species represented is about 230. These leaflets are dislributed …

In-Clinic Versus Online Recruitment Of Women With A History Of Cervical Intraepithelial Neoplasia Or Cervical Cancer To A Smoking Cessation Trial: A Post Hoc Comparison Of Participant Characteristics, Study Retention, And Cessation Outcomes., Bethany Shorey Fennell, Sarah R. Jones, Steven K. Sutton, Charles E. Hoogland, Cherell Cottrell-Daniels, David W. Wetter, Ya-Chen Tina Shih, Vani N. Simmons, Yesenia P. Stephens, Damon J. Vidrine, Jennifer I. Vidrine

Markey Cancer Center Faculty Publications

INTRODUCTION: Recruiting special populations to smoking cessation trials is challenging and approaches beyond in-clinic recruitment may be beneficial. This secondary analysis of data from a smoking cessation RCT for individuals with a history of cervical cancer or cervical intraepithelial neoplasia (CIN) explored differences associated with in-clinic vs. online recruitment.

AIMS AND METHODS: Participants were recruited from clinics within a university-based NCI-designated cancer center (n = 87) and online nationally via Facebook (n = 115). Baseline measures included sociodemographics, smoking history, and cancer or CIN history. Study retention and smoking abstinence were assessed 12 months post-baseline. Group differences in baseline characteristics …

Characterizing Gait In Young Male 5xfad Mice, Sophiya L. Sims, Kate E. Pauss, Sami L. Case, Ruei-Lung Lin, Leopoldine B. Galopin, Nicholas A. Wright, Olivier Thibault

Journal of Pharmacology & Nutritional Sciences

Gait control represents a non-cognitive domain that is affected negatively during late stages of Alzheimer’s Disease (AD) and AD Related Dementias (ADRDs). In some cases, early identification can be predictive of worsened cognitive function. Prompt detection could help define intervention windows, perhaps even years prior to formal diagnosis of dementia. In this study, the locomotor function of 3-month-old male 5xFAD mice was evaluated using several variables (speed, deviance from center, paw precision index, stride length, stride length deviation, and stride time) to characterize the change in ambulatory behavior prior to the onset of cognitive decline. The data presented here show …

Writing For The Journal: A Guide For Community-Based Organizations, Randy Wykoff, Rachel E. Dixon

Journal of Appalachian Health

The Journal of Appalachian Health welcomes submissions from a variety of stakeholders interested in and contributing to improvement of health across the Appalachian Region. This editorial provides basic guidelines for those working in community settings who may with to make JAH (or any other journal) their publication home.

Plant List Of Accepted Nomenclature, Taxonomy And Symbols, J S. Peterson

IGC Proceedings (1977-2023)

The Plant List of Accepted Nomenclature, Taxonomy & Symbols (PLANTS) is a dynamic database providing scientific and vernacular names, distributional, growth form, origin, symbols and bibliographic standardised botanical data to US federal agencies. PLANTS encompasses the vascular plant, moss, lichen and liverwort flora of North America, north of the Mexico border, including US states and territories.

Tactical Grazing Can Help Maintain Stability Of Semi-Arid Wooded Grasslands, K C. Hodgkinson

IGC Proceedings (1977-2023)

Arid and semi-arid wooded grasslands in Australia and elsewhere are prone to soil and plant productivity decline when used for pastoral production. Typically, degradation is characterised by woody plant increase, palatable perennial grass decline, unpalatable grass increase and lowered productive capacity of the soil, New grazing management that maintains productivity of plant and soil resources and sustains rehabilitation is required. In a grazing experiment on a mulga (Acacia aneura) woodland, grazing-induced death and impaired seed productivity were observed in palatable perennial grasses. A tactical grazing management to prevent an adverse change in pasture composition and productivity was designed involving progressively …

Sustainability Of African Farming Systems With Particular Reference To Soil Fertility, Multiple Cropping Systems And Weed Ingress In Smallholder Systems In Humid Tropical Africa, Bede N. Okigbo, Elly N. Sabiti

IGC Proceedings (1977-2023)

Africa continues to face a food and agricultural crisis, the effects of which are sometimes exacerbated by drought and civil strife. The food crisis is the result of the inability of African countries in the humid tropical areas and other zones to produce enough food to satisfy escalating demand, owing largely to rapid population growth and pressures of modernisation. Furthermore, the inability of these countries to earn adequate incomes and the lack of food security, especially in some countries suffering from drought, civil strife or war, has resulted in increasing reliance on food aid to fulfil their food import requirements. …

Genetic Improvement And Nutritive Value Of Lucerne: Crude Protein Content, P Rotili, G Gnocchi, C Scotti

IGC Proceedings (1977-2023)

Stratigraphical analysis of a lucerne (Medicago saliva L.) stand was made to show the effect of leaf age and health, and of leaf-stem ratio, on crude protein content, These data show that when the analysis was made on stems of the same age and on leaves of the same age, health and physiological state, the variability of this trait is negligible, which makes it very difficult to breed for crude protein content. Nevertheless ii is possible to improve the protein content by selection for physiological trails such as leaf persistence and tolerance of early culling.

Determining The Key Education Priorities Related To Heart Failure Care In Nursing Homes: A Modified Delphi Approach, James Mcmahon, David R. Thompson, Christine Brown Wilson, Loreena Hill, Paul Tierney, Jan Cameron, Doris S. F. Yu, Debra K. Moser, Karen Spilsbury, Nittaya Srisuk, Jos M. G. A. Schols, Mariëlle Van Der Velden-Daamen, Gary Mitchell

UK CARES Faculty Publications

There is currently a limited understanding of what nurses in nursing homes view as the key education priorities to support their ability to provide the appropriate care for residents with heart failure (HF). A modified Delphi technique was utilized to gain a consensus on the key education priorities for nurses working in nursing homes in Northern Ireland. An initial list of items (n = 58), across 19 domains, was generated using the findings of a scoping review and stakeholder interviews, and a review of available clinical guidelines. Two rounds of surveys were undertaken. Items were presented using a 5-point Likert …

Forage News [2024-08], University Of Kentucky

Forage News

No abstract provided.

Asap: A Pharmacy-Level Intervention To Increase Nonprescription Syringe Sales To Reduce Bloodborne Illnesses., B. E. Meyerson, J. Agley, Richard A. Crosby, K. G. Bentele, N. Vadiei, L. B. Linde-Krieger, D. R. Russell, K. Fine, L. A. Eldridge

Health, Behavior & Society Faculty Publications

BACKGROUND: Pharmacy syringe sales are effective structural interventions to reduce bloodborne illnesses in populations, and are legal in all but two states. Yet evidence indicates reduced syringe sales in recent years. This study was designed as a feasibility test of an intervention to promote syringe sales by pharmacies in Arizona.

METHODS: A four-month pilot among three Arizona pharmacies measured feasibility and acceptability through monthly surveys to 18 enrolled pharmacy staff members.

RESULTS: Pharmacy staff reported increased ease of dispensing syringes across the study. Rankings of syringe dispensing as 'easiest' among 6 measured pharmacy practices increased from 38.9 % at baseline …

Potential Impact Of Curative And Preventive Interventions Toward Hepatitis C Elimination In People Who Inject Drugs-A Network Modeling Study., Lin Zhu, William W. Thompson, Liesl Hagan, Liisa M. Randall, Abby E. Rudolph, April M. Young, Jennifer R. Havens, Joshua A. Salomon, Benjamin P. Linas

Center on Drug and Alcohol Research Faculty Publications

BACKGROUND: Injection-equipment-sharing networks play an important role in hepatitis C virus (HCV) transmission among people who inject drugs (PWID). Direct-acting antiviral (DAA) treatments for HCV infection and interventions to prevent HCV transmission are critical components of an overall hepatitis C elimination strategy, but how they contribute to the elimination outcomes in different PWID network settings are unclear.

METHODS: We developed an agent-based network model of HCV transmission through the sharing of injection equipment among PWID and parameterized and calibrated the model with rural PWID data in the United States. We modeled curative and preventive interventions at annual coverage levels of …

Buprenorphine Prescribing Characteristics Following Relaxation Of X-Waiver Training Requirements., Paul J. Christine, Rouba A. Chahine, Simeon D. Kimmel, Nicole Mack, Christian Douglas, Thomas J. Stopka, Katherine Calver, Laura C. Fanucchi, Svetla Slavova, Michelle R. Lofwall, Daniel J. Feaster, Michael Lyons, Jerel M. Ezell, Marc R. Larochelle

Center on Drug and Alcohol Research Faculty Publications

IMPORTANCE: Local-level data are needed to understand whether the relaxation of X-waiver training requirements for prescribing buprenorphine in April 2021 translated to increased buprenorphine treatment.

OBJECTIVE: To assess whether relaxation of X-waiver training requirements was associated with changes in the number of clinicians waivered to and who prescribe buprenorphine for opioid use disorder and the number of patients receiving treatment.

DESIGN, SETTING, AND PARTICIPANTS: This serial cross-sectional study uses an interrupted time series analysis of 2020-2022 data from the HEALing Communities Study (HCS), a cluster-randomized, wait-list-controlled trial. Urban and rural communities in 4 states (Kentucky, Massachusetts, New York, and Ohio) …

Associations Between Il-1Β, Il-6, And Tnfα Polymorphisms And Longitudinal Trajectories Of Cognitive Function In Non-Demented Older Adults, Karen A. Lawrence, Elana M. Gloger, Cristina N. Pinheiro, Frederick A. Schmitt, Suzanne C. Segerstrom

Neurology Faculty Publications

Inflammation is implicated in Alzheimer’s disease (AD), and specific single nucleotide polymorphisms (SNPs) in inflammatory cytokine genes are associated with increased AD risk. Whether the same polymorphisms also predict domain-specific cognitive change in cognitively healthy older adults is unclear. Specific SNPs in three cytokine genes, IL-1β (rs16944), IL-6 (rs1800795), and TNFα (rs1800629) were assessed for association with longitudinal trajectories spanning up to 16 years of global cognitive function, episodic memory, attention and working memory, and executive function in a sample of 324 non-demented older adults. Only rs1800629 (TNFα) was associated with significant change in global cognitive function over time [γ …

The Impact Of Social Deprivation On Health Care Utilization Patterns Following Closed Reduction And Percutaneous Pinning Of Pediatric Supracondylar Humerus Fractures, Elizabeth Cinquegrani, Matthew Van Boxtel, Sergey Tarima, Jessica Hanley

Biostatistics Faculty Publications

Background: Disparities in social determinants of health have been linked to worse patient-reported outcomes, higher postoperative pain, and increased risk of revision surgery following orthopaedic procedures. Identification of perioperative predictors of health care utilization is of particular interest to mitigate cost and improve patient outcomes. The aim of this study is to elucidate the effect of social deprivation levels, using the Area Deprivation Index (ADI), on health care utilization following the pinning of supracondylar humerus (SCH) fractures. Identifying risk factors for discrepancies in health care utilization following SCH fracture fixation can help mitigate unnecessary health care spending and improve the …

A Practical Guide To Data Management And Sharing For Biomedical Laboratory Researchers., K Fouad, R Vavrek, M C Surles-Zeigler, J R Huie, H L Radabaugh, G G Gurkoff, U Visser, J S Grethe, M E Martone, A R Ferguson, John C. Gensel, A Torres-Espin

Physiology Faculty Publications

Effective data management and sharing have become increasingly crucial in biomedical research; however, many laboratory researchers lack the necessary tools and knowledge to address this challenge. This article provides an introductory guide into research data management (RDM), and the importance of FAIR (Findable, Accessible, Interoperable, and Reusable) data-sharing principles for laboratory researchers produced by practicing scientists. We explore the advantages of implementing organized data management strategies and introduce key concepts such as data standards, data documentation, and the distinction between machine and human-readable data formats. Furthermore, we offer practical guidance for creating a data management plan and establishing efficient data …

Cerebrovascular Reactivity Mri As A Biomarker For Cerebral Small Vessel Disease–Related Cognitive Decline: Multi-Site Validation In The Markvcid Consortium, Peiying Liu, Zixuan Lin, Kaisha Hazel, George Pottanat, Cuimei Xu, Dengrong Jiang, Jay J. Pillai, Emma Lucke, Christopher E. Bauer, Brian T. Gold, Steven M. Greenburg, Karl G. Helmer, Kay Jann, Gregory Jicha, Joel Kramer, Pauline Maillard, Rachel M. Mulavelil, Pavel Rodriguez, Claudia L. Satizabal, Kristin Schwab, Sudha Seshadri, Herpreet Singh, Ángel G. Velarde Dediós, Danny J. J. Wang, Rita R. Kalyani, Abhay Moghekar, Paul B. Rosenberg, Sevil Yasar, Marilyn Albert, Hanzhang Lu

Neurology Faculty Publications

INTRODUCTION: Vascular contributions to cognitive impairment and dementia (VCID) represent a major factor in cognitive decline in older adults. The present study examined the relationship between cerebrovascular reactivity (CVR) measured by magnetic resonance imaging (MRI) and cognitive function in a multi-site study, using a predefined hypothesis.

METHODS: We conducted the study in a total of three analysis sites and 263 subjects. Each site performed an identical CVR MRI procedure using 5% carbon dioxide inhalation. A global cognitive measure of Montreal Cognitive Assessment (MoCA) and an executive function measure of item response theory (IRT) score were used as outcomes.

RESULTS: CVR …

Transcriptomics Analysis Reveals Potential Regulatory Role Of Nsmase2 (Smpd3) In Nervous System Development And Function Of Middle-Aged Mouse Brains, Zhihui Zhu, Timothy S. Mcclintock, Erhard Bieberich

Markey Cancer Center Faculty Publications

Neutral sphingomyelinase-2 (nSMase2), gene name sphingomyelin phosphodiesterase-3 (Smpd3), is a key regulatory enzyme responsible for generating the sphingolipid cer- amide. The function of nSMase2 in the brain is still controversial. To better under- stand the functional roles of nSMase2 in the aging mouse brain, we applied RNA-seq analysis, which identified a total of 1462 differentially abundant mRNAs between +/fro and fro/fro, of which 891 were increased and 571 were decreased in nSMase2-deficient mouse brains. The most strongly enriched GO and KEGG annota- tion terms among transcripts increased in fro/fro mice included synaptogenesis, syn- apse development, synaptic signaling, axon development, and …

Cigarette Smoke-Induced Epithelial-To-Mesenchymal Transition: Insights Into Cellular Mechanisms And Signaling Pathways, Sarah Mohammed Alqithami, Amrita Machwe, David K. Orren

Markey Cancer Center Faculty Publications

This review delves into the molecular complexities underpinning the epithelial-to-mesenchymal transition (EMT) induced by cigarette smoke (CS) in human bronchial epithelial cells (HBECs). The complex interplay of pathways, including those related to WNT//β-catenin, TGF-β/SMAD, hypoxia, oxidative stress, PI3K/Akt, and NF-κB, plays a central role in mediating this transition. While these findings significantly broaden our understanding of CS-induced EMT, the research reviewed herein leans heavily on 2D cell cultures, highlighting a research gap. Furthermore, the review identifies a stark omission of genetic and epigenetic factors in recent studies. Despite these shortcomings, the findings furnish a consolidated foundation not only for the …

Cryo-Em Reconstruction Of Oleate Hydratase Bound To A Phospholipid Membrane Bilayer, Michael L. Oldham, M. Zuhaib Qayyum, Ravi C. Kalathur, Charles O. Rock, Christopher D. Radka

Markey Cancer Center Faculty Publications

Oleate hydratase (OhyA) is a bacterial peripheral membrane protein that catalyzes FAD-dependent water addition to membrane bilayer-embedded unsaturated fatty acids. The opportunistic pathogen Staphylococcus aureus uses OhyA to counteract the innate immune system and support colonization. Many Gram-positive and Gram-negative bacteria in the microbiome also encode OhyA. OhyA is a dimeric flavoenzyme whose carboxy terminus is identified as the membrane binding domain; however, understanding how OhyA binds to cellular membranes is not complete until the membrane-bound structure has been elucidated. All available OhyA structures depict the solution state of the protein outside its functional environment. Here, we employ liposomes to …

Nsd3::Nutm1 Fusion Sarcoma Mimicking Malignant Peripheral Nerve Sheath Tumor With Prolonged Survival, Jing Di, Ali M. Alhaidary, Chi Wang, Jinge Liu, Sainan Wei, Joseph Valentino, Therese J. Bocklage

Markey Cancer Center Faculty Publications

Nuclear Protein in Testis (NUT)-rearranged tumors comprise predominantly NUT car- cinoma but also include certain lymphomas, leukemias, skin appendage tumors, and sarcomas. Although histologically diverse, all are genetically identified by oncogenic rearrangement in the NUTM1 gene. Many fusion partners occur, and NSD3 is NUT carcinoma’s third most common partner. Herein, we present a case of a 26-year-old man with an NSD3::NUTM1 fusion sarcoma. The patient presented at the age of 13 months with a scalp nodule. Over the next 24 years, he experienced five local recurrences and ultimately expired of a rapidly progressive recurrence. His treatment included surgical resections, radiation, …

Effects Of A Proprietary Kelp Blend Product On Enteric Methane Production And Tissue Residues In Cattle, Alexander Altman, Eric Vanzant, Sydney Davis, David Harmon, Kyle R. Mcleod

Animal and Food Sciences Faculty Publications

Three experiments were performed investigating bovine enteric methane (CH₄) production inhibition using a proprietary kelp blend product (PKBP) containing a halogenated methane analog (i.e., bromoform). Calves were fed a corn-silage basal diet top-dressed with the assigned treatment, with rations provided at 1.5 × NE_m in Experiments 1 and 2 (n = 12 and 6 steers, respectively) and ad libitum in Experiment 3 (n = 9 steers). In Experiment 1, we evaluated bromoform’s potency in decreasing CH₄. Dry matter intake (DMI) was not affected by treatment (p ≥ 0.11; 0 vs. 52.5 ± 10.5 ppm bromoform), …

Adhesion G Protein-Coupled Receptor Latrophilin 1 (Adgrl1): A Novel Regulator Of Glucose And Energy Homeostasis., Kavaljit H Chhabra

Pharmacology and Nutritional Sciences Faculty Publications

No abstract provided.

Antimicrobial Resistance In Equines: A Growing Threat To Horse Health And Beyond-A Comprehensive Review., Ajran Kabir, Bibek Lamichhane, Tasmia Habib, Alexis Adams, Hossam El-Sheikh Ali, Nathan M Slovis, Mats H. T. Troedsson, Yosra A. Helmy

Veterinary Science Faculty Publications

The equine industry holds substantial economic importance not only in the USA but worldwide. The occurrence of various infectious bacterial diseases in horses can lead to severe health issues, economic losses, and restrictions on horse movement and trade. Effective management and control of these diseases are therefore crucial for the growth and sustainability of the equine industry. While antibiotics constitute the primary treatment strategy for any bacterial infections in horses, developing resistance to clinically important antibiotics poses significant challenges to equine health and welfare. The adverse effects of antimicrobial overuse and the escalating threat of resistance underscore the critical importance …

Dairy Production From Pasture In Southern China, P.N P. Matthews, J Hodgson, A.C P. Chu, W L. Jiang, C B. Luo

IGC Proceedings (1977-2023)

This paper covers the development of dairy production systems as part of the UNDP funded Agro-grasslands Systems Development Project in Guizhou Province, China. The basic philosophy for the project was for local adaptation of New Zealand technology for developing dairy production systems based on pastures. The dairy production systems were established and the outputs of the project successfully achieved. Net incomes of smallholder farmers increased from a baseline of RMB $140 to RMB $1740 per annum over a three year period. Based on the experienced gained in setting up these systems local researchers, administrators, extension personnel and smallholders were able …

Forage-Based Seasonal Dairying In South Louisiana, H A. Deramus, L Labbe, K Ingawa, W A. Atkinson, M J. Simon, J D. Roussel

IGC Proceedings (1977-2023)

The effects on the net profit and total milk production were compared using two levels of grain supplementation and forage-only feeding in a program of seasonal dairying in South Louisiana using management intensive grazing. Treatments were 8 kg grain, 4 kg grain, and 0 grain supplementation. Annual ryegrass (Lolium multiflorium) and clovers (Trifolium incarnatum, and T. Alexandrinum) were grazed intensively with 51 multiparous Jersey and Holstein cows in mid-lactation in 1993-1995. Milk production, milk fat, milk protein, and blood urea nitrogen were measured. Milk and protein models were all highly significant for all classes. Daily milk …

Elevating Plk1 Overcomes Beti Resistance In Prostate Cancer Via Triggering Brd4 Phosphorylation-Dependent Degradation In Mitosis, Yanquan Zhang, Ka-Wing Fong, Fengyi Mao, Ruixin Wang, Derek B. Allison, Dana Napier, Daheng He, Jinpeng Liu, Yeqing Zhang, Jing Chen, Yifan Kong, Chaohao Li, Guangbing Li, Jinghui Liu, Zhiguo Li, Haining Zhu, Chi Wang, Xiaoqi Liu

Markey Cancer Center Faculty Publications

Bromodomain-containing protein 4 (BRD4) has emerged as a promising therapeutic target in prostate cancer (PCa). Understanding the mechanisms of BRD4 stability could enhance the clinical response to BRD4-tar- geted therapy. In this study, we report that BRD4 protein levels are significantly decreased during mitosis in a PLK1-dependent manner. Mechanistically, we show that BRD4 is primarily phosphorylated at T1186 by the CDK1/cyclin B complex, recruiting PLK1 to phosphorylate BRD4 at S24/S1100, which are recognized by the APC/CCdh1 complex for proteasome pathway degradation. We find that PLK1 overexpression lowers SPOP mutation-stabilized BRD4, consequently rendering PCa cells re-sensitized to BRD4 inhibitors. Intrigu-ingly, we …

Effects Of Two Grazing Conditions On The Reproductive Achievement Of Male Alpaca In The Northern Peru Grasslands, C Pinares, E Malpartida, S Cortez

IGC Proceedings (1977-2023)

Alpaca have recently been introduced to the northern Peru grasslands (Jalca). Effects of two systems of grazing management, co-grazing (with sheep) under fair grassland condition (CGFC) versus exclusive grazing under good grassland condition (EGGC), upon the reproductive achievement was examined from the 6th to the 16th month of age. Each system involved 20 Huacaya animals, managed by individual herders. Availability of herbage preferred by alpaca (HMA) and animal measurements (herbage intake, liveweight, testicle size, penis release from preputial attachments, plasma testosterone) were monitored monthly. Puberty, judged by the penis release from the preputial attachments, was achieved by 7 …