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Comparative Mapping: Homology Within The Order Perissodactyla Of Four Genes Located On Equus Caballus Chromosome 20, Christine Marie Mains

University of Kentucky Master's Theses

Since changes in chromosome morphology contribute to the knowledge of evolution as well as to chromosome dynamics, this study looks specifically at one chromosome compared in twelve different species of Perissodactyls: Equus caballus (ECA), E. przewalskii (EPR), Equus africanus somaliensis (EAF), E. asinus (EAS), E. hemionus onager (EHO), E. h. kulan (EHK), E. h. kiang (EKI), E. zebra hartmannae (EZH), E. grevyi (EGR), E. burchelli (EBU), Tapirus indicus (TIN), and Rhinoceros unicornis (RUN). While chromosome morphology studies have been done in some of the extant equids, none have followed the evolution of this chromosome, homologous to Equus caballus chromosome 20 …

Supernatant Protein Factor: Insights Into Its Regulation And Ability To Stimulate Cholesterol Synthesis In Vitro And In Cell Culture, Vishwesh Mokashi

University of Kentucky Doctoral Dissertations

Supernatant protein factor (SPF) is a 46-kDa cytosolic protein that stimulates squalene monooxygenase, which catalyses the second committed step in cholesterol biosynthesis. The mechanism by which SPF stimulates this enzyme is not understood and the goal of these studies was to see if SPF affected cholesterol synthesis in cultured cells. Rat supernatant protein factor-like protein (SPF2) shares 77% sequence identity with human SPF. In my studies SPF2 also stimulated squalene monooxygenase in vitro and incubation of SPF2 with protein kinase A (PKA) and C increased its activity by about 2-fold, as shown earlier with SPF. GTP and GDP prevented the …

Mechanisms Of Resistance To Halogenated And Non-Halogenated Ahr Ligands In Chronically Contaminated Killifish Populations, Xabier Arzuaga

University of Kentucky Doctoral Dissertations

Chronically contaminated killifish from Newark Bay (NB) NJ, and New Bedford Harbor (NBH) MA, have developed resistance to halogenated aromatic hydrocarbons that bind to and activate the aryl hydrocarbon receptor (AHR). To study the mechanisms of resistance, adult killifish were exposed to halogenated and non-halogenated AHR ligands and enzymatic and toxicological endpoints were measured in adult and embryonic fish.

The chlorinated and non-chlorinated AHR ligands 3,34,4-tetrachlorobiphenyl (PCB77) and benzo-a-pyrene (B[a]P) induced cytochrome P450 1A (CYP1A) in reference site, but not in NB killifish. Expression of CYP3A (not part of the AHR gene battery) was inducible only in Flax Pond killifish. …

Regulation Of Concentrated Animal Feeding Operations: An Application Of Economic Theory To Firm Decision Making And Applications For Public Policy, Benjamin Gramig

University of Kentucky Master's Theses

The livestock industry in the United States has experienced significant concentration and vertical integration in recent years. This change has resulted in greater observed levels of pollution attributed to concentrated animal feeding operations (CAFO) and society has attempted to use regulation to remedy these problems. Despite regulation at the federal and local level no documented improvement in water quality has been observed to date. This thesis is concerned with the response of profit-maximizing economic agents to the form of environmental regulation adopted at the federal level. A theoretical model of firm profit is proposed and analyzed using comparative statics to …

Breeding For Fusarium Head Blight Resistance In Soft Red Winter Wheat, Virginia Laura Verges

University of Kentucky Master's Theses

Fusarium graminearum, the causative agent of Fusarium head blight, is an economically important pathogen of wheat (Triticum aestivum). Breeding Fusarium head blight (FHB) resistant wheat requires knowledge of the underlying genetic control of FHB resistance. Genetic parameters for FHB resistance and five related traits were estimated in three populations at two locations and in two years. Moderate broad sense heritabilities for FHB severity and Fusarium damaged kernels (FDK) were observed. Incidence of FHB and the toxin deoxynivalenol (DON) accumulation had low to moderate broad sense heritabilities. Correlations between FDK and severity and FDK and DON were moderate to high in …

Challenges In Estimating Size And Conservation Of Black Bear In West-Central Florida, Joshua Hager Brown

University of Kentucky Master's Theses

The Greater Chassahowitkza Ecosystem black bear (Ursus americanus floridanus) population of west-central Florida is likely to be the smallest documented population of the species. It has experienced almost no recruitment since 1997 and exhibits behavior that appears to be a response to human activities. The local diet is dominated by the fruit of saw palmetto and sabal palm, species that exhibit patchy distributions and irregular mast production. These food supplies are often separated by busy highways that have killed 6 bears since 1997, 21% of known individuals. Motion-activated camera surveys suggest that the bear population is declining in this rapidly …

Perceptions Of Quality Of Life And Use Of Human Services By Households: A Model, Baomei Zhao

University of Kentucky Doctoral Dissertations

Quality of life is a term that has been used in various ways by researchers in different fields. In regional or community research, researchers have been concerned to a large extent with a persons overall quality of life as affected by factors such as income, housing, marital status, gender, and regional/community human services. The present research concerns the relationship between perception of quality of life and the use of human services in the community. The data were from Lexington-Fayette County, Kentucky. Family systems theory served as the underlying conceptual framework for this study. Family systems theory would predict that residents …

The Social Life Of Poetry: Pluralism And Appalachia, 1937-1946, Christopher Allen Green

University of Kentucky Doctoral Dissertations

This dissertation demonstrates how poetry about Appalachia expanded American considerations of democracy, ethnicity, and cultural values. I argue that poetry is profoundly communal in its construction and investigate how the value of poetry changes based upon its transfer through varying networks of production, circulation, and reception. Informed by theories of cultural capital and rhetoric, the chapters trace three books of poetry from their composition and publication to their reception and influence, noting how central political and social institutions and individuals shaped that process. The dissertation establishes how the poets crafted their writing to sway specific interpretive communities attitudes on pluralism. …

Finite Element Analysis And Reliability Study Of Multi-Piece Rims, Sandeep Chodavarapu

University of Kentucky Master's Theses

Multi-piece wheels or rims used on large vehicles such as trucks, tractors, trailers, buses and off-road machines have often been known for their dangerous properties because of the large number of catastrophic accidents involving them. The main causes for these accidents range from dislocation of the rim components in the assembly, mismatch of the components, manufacturing tolerances, corrosion of components to tires. A finite element analysis of a two-piece rim design similar to one manufactured by some of the prominent rim manufacturers in the USA is undertaken. A linear static deformation analysis is performed with the appropriate loading and boundary …

Failure Rate Studies And Design Alternatives For Standup Forklift Trucks, Srinivas Jagarlamudi

University of Kentucky Master's Theses

Standup forklift trucks are extensively used primarily for material handling in high density warehouses. These forklifts over the years have been involved in severe accidents causing injuries and taking lives of the operator and that of people on the floor. The major accidents involving these trucks are tip-over, off the dock accidents, compartment intrusions and under the rack injuries. The objective of the work is to analyze the accident data and to provide a conceptual design to ensure safety of the operator riding the standup forklift trucks. The operator is assumed to be safe when retained within the compartment similar …

Investigation Of Characteristics Of Bounded Wall Jets In Dead End Mine Headings, Lavanya Rangubhotla

University of Kentucky Master's Theses

A comprehensive experimental study has been conducted using Particle Image Velocimetry (PIV) for a wide array of ventilation schemes and mining configurations for the purpose of examining ventilation characteristics in dead end mine headings. Flow behaviors in two basic mining sequences of box and slab cuts for 30 ft and 60 ft deep cuts were studied. The present thesis discusses the effect for various geometric and flow parameters including the variation of inlet flow velocities, entry heights, face zone widths and curtain widths on the flow behavior. The Reynolds number Re considered for this study ranges from 1 105 to …

Global Change, Domestic Policy, And Life Course Influences On Perceptions Of Health Equity Among Older Cubans, James Lester Schwar

University of Kentucky Doctoral Dissertations

Cubas provision of free health services to the entire population via neighborhood-based family doctors produced dramatic health gains and achieved a relative state of health equality. Since 1989, however, the termination of Soviet trade, a grave economic crisis, intensification of the US embargo, welfare reductions, and population aging have placed Cubas health successes and elder care services in jeopardy. Little independent research, though, has focused on the influence of post-Cold War circumstances on citizen attitudes about health programs and resources targeting Cubas older population. This research examined global and domestic factors since 1989 that have most influenced perceptions of the …

Piano Inside Out: The Expansion Of The Expressive, Technical, And Sonorous Spectrum In Selected Twentieth-Century Art-Music Repertoire For The Modern Acoustic Piano, Mira Kruja

University of Kentucky Doctoral Dissertations

Art Music for the acoustic piano has changed tremendously during the twentieth century. Some of the techniques and skills pianists need to master in order to be able to perform successfully twentieth-century art piano music include: a refined ability to discriminate varied layers of sonorities; sophisticated pedal combinations; a sometimes percussive technique; and superior control of complex metric and rhythmic passages. New combinations of patterns that require specific technical preparation pose substantial pianistic challenges. Todays pianist needs to master a variety of glissandi, chords, or single melody textures played directly on the strings inside the piano and to combine such …

Molecular Functionalization Of Carbon Nanotubes And Use As Substrates For Neuronal Growth, Mark P. Mattson, Robert C. Haddon, Apparao M. Rao

Sanders-Brown Center on Aging Faculty Patents

A cell and substrate system and nerve regeneration implant are disclosed including a carbon nanotube and a neuron growing on the carbon nanotube. Both unfunctionalized carbon nanotubes and carbon nanotubes functionalized with a neuronal growth promoting agent may be utilized in the invention. A method is also disclosed for promoting neuronal growth.

Dual Stage Microvalue And Method Of Use, Marc J. Madou, Sylvia Daunert

Chemistry Faculty Patents

A micro-machined drug delivery device and method of use for the delivery of labile drugs is disclosed. A micro-machined sensing device and method of use is also disclosed. A micro-machined drug delivery and sensing device and method of use is additionally disclosed. All three devices are intended to be inserted into a patient's body. The drug delivery devices allow for the mixing of drug components prior to the release of the mixture into the patient's body where the mixture is labile. The micro-machined sensing device is suitable for monitoring the concentration of a specific chemical in a patient's body fluids …

Impaired Antigen Receptor Induced Calcium Mobilization In A Phospholipase C-Gamma1 Deficient B Cell Line., Natarajan Muthusamy, Do Joon Park, Hei-Won Rho, Sue Goo Rhee, Subbarao Bondada

Microbiology, Immunology, and Molecular Genetics Faculty Publications

B lymphocytes express phospholipase C-gamma(1) (PLC-gamma(1)) and phospholipase C-gamma(2) (PLC-gamma(2)) isozymes. However, the relative importance of these two isozymes in B cell signaling is not known. We report here the identification and analysis of a B cell line deficient in PLC-gamma(1). Mature splenic B lymphocytes and a panel of cell lines representing pre-B, immature and mature B cell stages expressed phospholipase C-gamma (PLC-gamma), but not the beta or delta isoforms of phospholipase C (PLC). While all the tested B cell lines and primary splenic B cells expressed PLC-gamma(1) and PLC-gamma(2) isozymes, the L1.2 B cell line exclusively expressed PLC-gamma(2), but …

Heavy Equipment Owner/Operators Dying On The Job, Kentucky Injury Prevention And Research Center

Kentucky Haz Alerts--Other

To prevent heavy equipment injuries:

• Owner/operators should become familiar with and adhere to Kentucky Occupational Safety and Health guidelines.
• Manufacturers’ procedures for operation and maintenance of heavy equipment should be followed.
• Avoid potentially hazardous situations. Operators should perform a thorough hazard assessment before work is initiated.

Propietarios/Operadores De Equipo Pesado Mueren En Su Trabajo, Kentucky Injury Prevention And Research Center

Kentucky Haz Alerts--Motor Vehicle

Para prevenir lesiones al trabajar con equipo pesado:

1. Propietarios/operadores deberán familiarizarse y aplicar las normas de Seguridad y Salud Ocupacional de Kentucky.
2. Deberán ser seguidos los procedimientos indicados por el fabricante para la operación y mantenimiento de equipo pesado.
3. Evite situaciones que impliquen un riesgo potencial. Los operadores deberán llevar a cabo una valoración cuidadosa de los riesgos potenciales antes de iniciar el trabajo.

Fiscal Year 2003 Fhwa-536 Report For The Kentucky Transportation Cabinet, Steven Douglas Kreis

Kentucky Transportation Center Research Report

The Federal Highway Administration (FHWA) requires state transportation agencies to submit a biennial report on local highway finances. The purpose of these reports is to provide FHWA with the data it needs to capture the financing of highway activities at the local level. Based on this information, it can identify trends in revenue, expenditures, investments, and program development, and in turn make decisions about future investments. The report, FHWA-536, asks agencies to report on four areas of local highway finance: 1) disposition of highway-user revenues; 2) revenues used for roads and streets identified by source and funding type; 3) road …

Efficient Grazing Systems: Putting Pieces Together, Charles T. Dougherty

Kentucky Grazing Conference

Pros and cons of grazing systems are well-known to this audience. Efficient grazing systems, however, are a different story. What is an efficient grazing system? I think we could argue that topic until the cows come home and I do not believe we could come to any agreement. I am going to approach the subject of efficiency from several angles but you will probably see them differently. Economic efficiencies will not be covered.

Kentucky Goat Friendly Pasture Concepts, Terry Hutchens

Kentucky Grazing Conference

Goats are accomplished grazers of grass and browse plants. In addition, they can be managed within a grazing system both intensively or extensively as long as quality forage is made available. Goats are facultative browsers, they prefer to feed at eye level and upward and then feed on forage from the top of the plant down. Goats prefer to move freely from plant to plant-removing foliage from select portions of plants. Foliage meals are dictated by quality factors that insure adequate protein and energy levels. Each stem or leaf is clipped with precision leaving the forage residue standing equal in …

Pharmaceutical Formulation For Poorly Water Soluble Camptothecin Analogues, Tiang-Xiang Xiang, Bradley D. Anderson

Pharmaceutical Sciences Faculty Patents

The present invention provides a general method to retard the precipitation inception time for poorly water-soluble camptothecin analogues from a supersaturated solution by a chemical conversion approach via pH alteration. This method is successfully utilized to prepare stable parenteral formulations for silatecan 7-t-butyldimethylsilyl-10-hydroxycamptothecin (DB-67), a poorly water-soluble lipophilic camptothecin analogue, in aqueous solutions containing β-cyclodextrin sulfobutyl ether (SBE-CD) or other solubilizing agents. The formulations manufactured by this method are more simple and cost-effective, of higher doses and better quality in terms of manufacture loss and formulation stability, and can be free of organic solvents (e.g., DMSO or N-methyl-2-pyrrolidinone).

Synthases, Joseph Chappell, Kathleen R. Manna, Joseph P. Noel, Courtney M. Starks

Pharmaceutical Sciences Faculty Patents

Novel synthases and the corresponding nucleic acids encoding such synthases are disclosed herein. Such synthases possess an active site pocket that includes key amino acid residues that are modified to generate desired terpenoid reaction intermediates and products. Synthase modifications are designed based on, e.g., the three-dimensional coordinates of tobacco 5-epi-aristolochene synthase, with or without a substrate bound in the active site.

Protection Against Ischemia And Reperfusion Injury, Peter R. Oeltgen, Paul D. Bishop, Mark S. Kindy, Juan A. Sanchez

Molecular and Cellular Biochemistry Faculty Patents

A compound and method for using compound-D SEQ ID NO:1 to reduce injury associated with ischemia and reperfusion of mammalian organs such as the heart. The compound may be administered as part of a preconditioning strategy which reduces the extent of injury and improves organ function following cessation and restoration of blood flow. The compound may be used in preparation for planned ischemia or in a prophylactic manner in anticipation of further ischemic events.

Muriéndose Por Conducir - Colisiones Ocupacionales De Automotores En Kentucky, Kentucky Injury Prevention And Research Center

Kentucky Haz Alerts--Motor Vehicle

Para prevenir lesiones al trabajar con vehículos motorizados:

Los empleadores deben proporcionar programas de entrenamiento sobre seguridad al conducir, obligatorios y adaptados a sus empleados, que incluyan Reconocimiento de Síntomas de Distracción / Déficit de Atención del Conductor y Fatiga del Conductor.

Los empleados deben evitar distracciones y llevar siempre puesto el cinturón de seguridad.

Evite exponerse usted mismo a una situación potencialmente riesgosa. Los conductores deben siempre permanecer alerta y practicar Técnicas Defensivas de Conducción.

19th Annual Environmental Law Institute, Office Of Continuing Legal Education At The University Of Kentucky College Of Law

Continuing Legal Education Materials

Materials from the 19th Annual Environmental Law Institute held by UK/CLE in November 2003.

Dying To Drive – Occupational Motor Vehicle Collisions In Kentucky, Kentucky Injury Prevention And Research Center

Kentucky Haz Alerts--Motor Vehicle

To prevent motor vehicle injuries while working:

• Employers should provide mandatory comprehensive employee driver safety training programs including recognition of symptoms of driver distraction/inattention and driver fatigue.
• Employees should avoid distractions and wear seat belts at all times.
• Don’t place yourself in a potentially hazardous situation. Drivers should always stay alert and practice defensive driving techniques.

Content And Criterion Validity Evaluation Of National Public Health Performance Standards Measurement Instruments., Joyce Beaulieu, F. Douglas Scutchfield, Ann V. Kelly

Preventive Medicine and Environmental Health Faculty Publications

OBJECTIVE: The Centers for Disease Control and Prevention's National Public Health Performance Standards Program (NPHPSP) has developed instruments to measure the performance of local and state public health departments on the 10 "Essential Services of Public Health," which have been tested in several states. This article is a report of the evaluation of the content and criterion validity of the local public health performance assessment instrument, and the content validity of the state public health performance assessment instrument.

METHODS: Health department performance is measured using a set of indicators developed for the 10 Essential Services of Public Health and a …

Hispanic Laborer Dies From Fall Off Roof, Kentucky Injury Prevention And Research Center

Fatality Case Reports--Falls

On March 3, 2003, a 22-year-old Hispanic female laborer (decedent) died when she fell from a roof. She had been working on the roof with her brother, who was a sub-contractor for a local roofing company. The work crew consisted of two brothers and the decedent. Two days prior to the incident, the decedent had worked for her brother cleaning and organizing materials on the ground at another location. It was the decedent’s first day on this job site. It is unknown whether or not the decedent had worked on other roofing jobs. Her job was to organize materials and …

Bulldozer Owner/Operator Dies When Thrown Off Bulldozer Track, Kentucky Injury Prevention And Research Center

Fatality Case Reports--Other

On June 19, 2003, a 58-year-old bulldozer owner/operator (decedent) died when he was thrown off the track of his bulldozer. The bulldozer owner/operator and a laborer were having difficulty starting the bulldozer. Together, the two men had tried several different methods to start the bulldozer including: spraying ether into the air chamber, having the battery recharged, replacing the battery cables with new ones and tightening wires to the starter. They worked from the left side track of the bulldozer. The laborer sat towards the front of the track tightening the wires to the starter while the owner stood on the …