Digital Commons Network^™

Cyclic Di-Gmp-Dependent Signaling Pathways In The Pathogenic Firmicute Listeria Monocytogenes, Li-Hong Chen, Volkan K. Köseoğlu, Zehra T. Güvener, Tanya Myers-Morales, Joseph M. Reed, Sarah E. F. D'Orazio, Kurt W. Miller, Mark Gomelsky

Microbiology, Immunology, and Molecular Genetics Faculty Publications

We characterized key components and major targets of the c-di-GMP signaling pathways in the foodborne pathogen Listeria monocytogenes, identified a new c-di-GMP-inducible exopolysaccharide responsible for motility inhibition, cell aggregation, and enhanced tolerance to disinfectants and desiccation, and provided first insights into the role of c-di-GMP signaling in listerial virulence. Genome-wide genetic and biochemical analyses of c-di-GMP signaling pathways revealed that L. monocytogenes has three GGDEF domain proteins, DgcA (Lmo1911), DgcB (Lmo1912) and DgcC (Lmo2174), that possess diguanylate cyclase activity, and three EAL domain proteins, PdeB (Lmo0131), PdeC (Lmo1914) and PdeD (Lmo0111), that possess c-di-GMP phosphodiesterase activity. Deletion of all …

Forage News [2014-08], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Forage Tour Photos
• Kentucky Forage & Grassland Council Field Day
• Choose Varieties Wisely
• A Farmer's Creed
• Understanding the 'Intensive' in Intensive Grazing
• Minimize Alfalfa Yield Loss Due to Wheel Traffic
• Alfalfa Acres up 2.4%; All Hay Down

Closing A Liquid Manure Storage Structure, Stephen F. Higgins, Kylie Schmidt

Agriculture and Natural Resources Publications

Liquid manure storage structures, such as a lagoon, holding pond, or pit, serve an essential purpose on an active livestock operation. However, when this structure is no longer actively managed it can become a major liability to the producer because of its potential to have a discharge. The discharge from a liquid manure storage structure can contain pollutants such as nutrients, heavy metals, hormones, pathogens, and agriculture chemicals, all of which can pose serious threats to human health and aquatic ecosystems. Because of the pollution potential, livestock producers ceasing their operation are required to close their liquid manure storage structure(s) …

In Vivo Processing Of Ceria Nanoparticles Inside Liver: Impact On Free-Radical Scavenging Activity And Oxidative Stress, Uschi M. Graham, Michael T. Tseng, Jacek B. Jasinski, Robert A. Yokel, Jason M. Unrine, Burtron H. Davis, Alan K. Dozier, Sarita S. Hardas, Rukhsana Sultana, Eric A. Grulke, D. Allan Butterfield

Pharmaceutical Sciences Faculty Publications

The cytotoxicity of ceria ultimately lies in its electronic structure, which is defined by the crystal structure, composition, and size. Despite previous studies focused on ceria uptake, distribution, biopersistance, and cellular effects, little is known about its chemical and structural stability and solubility once sequestered inside the liver. Mechanisms will be presented that elucidate the in vivo transformation in the liver. In vivo processed ceria reveals a particle-size effect towards the formation of ultrafines, which represent a second generation of ceria. A measurable change in the valence reduction of the second-generation ceria can be linked to an increased free-radical scavenging …

Brassicas: Be Aware Of The Animal Health Risks, Michelle Arnold, Jeff Lehmkuhler

Agriculture and Natural Resources Publications

Winter annuals are often used to extend the grazing season. These forages can be used as supplemental feed when lower quality perennial forages dominate or to provide grazing at times when other forages are not available. On farms where row crops are grown, the combination of crop residues and fall growth of annual crops can allow livestock grazing to be extended well into the winter months. The annuals provide a cover for the soil during winter as well. Winter annuals planted in the early fall will allow grazing in late fall to early winter similar to stockpiled fescue. Based on …

Paramyxovirus Glycoprotein Incorporation, Assembly And Budding: A Three Way Dance For Infectious Particle Production, Farah El Najjar, Anthony P. Schmitt, Rebecca Ellis Dutch

Molecular and Cellular Biochemistry Faculty Publications

Paramyxoviruses are a family of negative sense RNA viruses whose members cause serious diseases in humans, such as measles virus, mumps virus and respiratory syncytial virus; and in animals, such as Newcastle disease virus and rinderpest virus. Paramyxovirus particles form by assembly of the viral matrix protein, the ribonucleoprotein complex and the surface glycoproteins at the plasma membrane of infected cells and subsequent viral budding. Two major glycoproteins expressed on the viral envelope, the attachment protein and the fusion protein, promote attachment of the virus to host cells and subsequent virus-cell membrane fusion. Incorporation of the surface glycoproteins into infectious …

Alcohol Promotes Mammary Tumor Growth Through Activation Of Vegf-Dependent Tumor Angiogenesis, Yanmin Lu, Fang Ni, Mei Xu, Jinlian Yang, Ji Chen, Zhuo Chen, Xinyi Wang, Jia Luo, Siying Wang

Internal Medicine Faculty Publications

Alcohol consumption has been recognized as a risk factor for breast cancer. Experimental studies demonstrate that alcohol exposure promotes the progression of existing mammary tumors. However, the mechanisms underlying this effect remain unclear. In the present study, the role of vascular endothelial growth factor (VEGF) in alcohol promotion of breast cancer development was investigated using a mouse xenograft model of mammary tumors and a three-dimensional (3D) tumor/endothelial cell co-culture system. For the mouse xenograft model, mouse E0771 breast cancer cells were implanted into the mammary fat pad of C57BL6 mice. These mice were exposed to alcohol in their drinking water. …

Novel Synergistic Protective Efficacy Of Atovaquone And Diclazuril On Fetal-Maternal Toxoplasmosis, Helieh S. Oz

Internal Medicine Faculty Publications

Over 1 billion people globally are estimated to be infected with Toxoplasma gondii with severe or unknown consequences and no safe and effective therapies are available against congenital or persistent chronic infection. We propose that atovaquone and diclazuril synergistically protect against fetal-maternal toxoplasmosis.

METHODS: Programmed pregnant mice were treated with atovaquone and diclazuril monotherapy, or combined (atovaquone + diclazuril) therapy and infected with tachyzoites (0, 300, 600) and the course of infection was studied.

RESULTS: Infected dams with low dose (300) developed moderate toxoplasmosis complications and treatments were similarly effective with minor differences between monotherapies. In contrast, major differences were …

Cardioprotection By Controlling Hyperamylinemia In A "Humanized" Diabetic Rat Model, Sanda Despa, Savita Sharma, Todd R. Harris, Hua Dong, Ning Li, Nipavan Chiamvimonvat, Heinrich Taegtmeyer, Kenneth B. Margulies, Bruce D. Hammock, Florin Despa

Pharmacology and Nutritional Sciences Faculty Publications

BACKGROUND: Chronic hypersecretion of the pancreatic hormone amylin is common in humans with obesity or prediabetic insulin resistance and induces amylin aggregation and proteotoxicity in the pancreas. We recently showed that hyperamylinemia also affects the cardiovascular system. Here, we investigated whether amylin aggregates interact directly with cardiac myocytes and whether controlling hyperamylinemia protects the heart.

METHODS AND RESULTS: By Western blot, we found abundant amylin aggregates in lysates of cardiac myocytes from obese patients, but not in controls. Aggregated amylin was elevated in failing hearts, suggesting a role in myocyte injury. Using rats overexpressing human amylin in the pancreas (HIP …

Rorα Binds To E2f1 To Inhibit Cell Proliferation And Regulate Mammary Gland Branching Morphogenesis, Gaofeng Xiong, Ren Xu

Markey Cancer Center Faculty Publications

Retinoic acid receptor-related orphan nuclear receptor alpha (RORα) is a potent tumor suppressor that reduces cell proliferation and inhibits tumor growth. However, the molecular mechanism by which it inhibits cell proliferation remains unknown. We demonstrate a noncanonical nuclear receptor pathway in which RORα binds to E2F1 to inhibit cell cycle progression. We showed that RORα bound to the heptad repeat and marked box region of E2F1 and suppressed E2F1-regulated transcription in epithelial cells. Binding of RORα inhibited E2F1 acetylation and its DNA-binding activity by recruiting histone deacetylase 1 (HDAC1) to the protein complexes. Knockdown of HDAC1 or inhibition of HDAC …

Persistent Hepatic Structural Alterations Following Nanoceria Vascular Infusion In The Rat, Michael T. Tseng, Qiang Fu, Khoua Lor, G. Rafael Fernandez-Botran, Zhong-Bin Deng, Uschi M. Graham, D. Allan Butterfield, Eric A. Grulke, Robert A. Yokel

Chemistry Faculty Publications

Understanding the long-term effects and possible toxicity of nanoceria, a widely utilized commercial metal oxide, is of particular importance as it is poised for development as a therapeutic agent based on its autocatalytic redox behavior. We show here evidence of acute and subacute adverse hepatic responses, after a single infusion of an aqueous dispersion of 85 mg/kg, 30 nm nanoceria into Sprague Dawley rats. Light and electron microscopic evidence of avid uptake of nanoceria by Kupffer cells was detected as early as 1 hr after infusion. Biopersistent nanoceria stimulated cluster of differentiation 3⁺ lymphocyte proliferation that intermingled with nanoceria-containing …

Neutral Sphingomyelinase-3 Mediates Tnf-Stimulated Oxidant Activity In Skeletal Muscle, Jennifer S. Moylan, Jeffrey D. Smith, Erin M. Wolf Horrell, Julie B. Mclean, Gergana M. Deevska, Mark R. Bonnell, Mariana N. Nikolova‑Karakashian, Michael B. Reid

Physiology Faculty Publications

AIMS: Sphingolipid and oxidant signaling affect glucose uptake, atrophy, and force production of skeletal muscle similarly and both are stimulated by tumor necrosis factor (TNF), suggesting a connection between systems. Sphingolipid signaling is initiated by neutral sphingomyelinase (nSMase), a family of agonist-activated effector enzymes. Northern blot analyses suggest that nSMase3 may be a striated muscle-specific nSMase. The present study tested the hypothesis that nSMase3 protein is expressed in skeletal muscle and functions to regulate TNF-stimulated oxidant production.

RESULTS: We demonstrate constitutive nSMase activity in skeletal muscles of healthy mice and humans and in differentiated C2C12 myotubes. nSMase3 ( …

Transition From An M1 To A Mixed Neuroinflammatory Phenotype Increases Amyloid Deposition In App/Ps1 Transgenic Mice, Erica M. Weekman, Tiffany L. Sudduth, Erin L. Abner, Gabriel J. Popa, Michael D. Mendenhall, Holly M. Brothers, Kaitlyn Braun, Abigail Greenstein, Donna M. Wilcock

Sanders-Brown Center on Aging Faculty Publications

BACKGROUND: The polarization to different neuroinflammatory phenotypes has been described in early Alzheimer's disease, yet the impact of these phenotypes on amyloid-beta (Aβ) pathology remains unknown. Short-term studies show that induction of an M1 neuroinflammatory phenotype reduces Aβ, but long-term studies have not been performed that track the neuroinflammatory phenotype.

METHODS: Wild-type and APP/PS1 transgenic mice aged 3 to 4 months received a bilateral intracranial injection of adeno-associated viral (AAV) vectors expressing IFNγ or green fluorescent protein in the frontal cortex and hippocampus. Mice were sacrificed 4 or 6 months post-injection. ELISA measurements were used for IFNγ protein levels and …

Maternal And Congenital Toxoplasmosis, Currently Available And Novel Therapies In Horizon, Helieh S. Oz

Internal Medicine Faculty Publications

Over one billion people worldwide are predicted to harbor Toxoplasma infection frequently with unknown lifelong health consequences. Toxoplasmosis is an important cause of foodborne, inflammatory illnesses, as well as congenital abnormalities. Ubiquitous Toxoplasma has a unique tropism for central nervous system with a mind-bugging effect and is transmitted sexually through semen. Currently available therapies are ineffective for persistent chronic disease and congenital toxoplasmosis or have severe side effects which may result in life-threatening complications. There is an urgent need for safe and effective therapies to eliminate or treat this cosmopolitan infectious and inflammatory disease. This investigation discusses pathogenesis of maternal …

Newborn Care: What We Can Learn From The Kangaroo Mother, Donald E. Greydanus, Joav Merrick

Pediatrics Faculty Publications

No abstract provided.

Cell Type-Dependent Rna Recombination Frequency In The Japanese Encephalitis Virus, Wei-Wei Chiang, Chingkai Chuang, Mei Chao, Wei-June Chen

Plant Pathology Faculty Publications

Japanese encephalitis virus (JEV) is one of approximately 70 flaviviruses, frequently causing symptoms involving the central nervous system. Mutations of its genomic RNA frequently occur during viral replication, which is believed to be a force contributing to viral evolution. Nevertheless, accumulating evidences show that some JEV strains may have actually arisen from RNA recombination between genetically different populations of the virus. We have demonstrated that RNA recombination in JEV occurs unequally in different cell types. In the present study, viral RNA fragments transfected into as well as viral RNAs synthesized in mosquito cells were shown not to be stable, especially …

Genome-Wide Determination Of Poly(A) Sites In Medicago Truncatula: Evolutionary Conservation Of Alternative Poly(A) Site Choice, Xiaohui Wu, Bobby Gaffney, Arthur G. Hunt, Qingshun Q. Li

Plant and Soil Sciences Faculty Publications

BACKGROUND: Alternative polyadenylation (APA) plays an important role in the post-transcriptional regulation of gene expression. Little is known about how APA sites may evolve in homologous genes in different plant species. To this end, comparative studies of APA sites in different organisms are needed. In this study, a collection of poly(A) sites in Medicago truncatula, a model system for legume plants, has been generated and compared with APA sites in Arabidopsis thaliana.

RESULTS: The poly(A) tags from a deep-sequencing protocol were mapped to the annotated M. truncatula genome, and the identified poly(A) sites used to update the annotations of 14,203 …

Postales Para El Olvido, Alberto Bejarano

Nomenclatura: aproximaciones a los estudios hispánicos

No abstract provided.

Neuroinflammation And Neurodegeneration In Adult Rat Brain From Binge Ethanol Exposure: Abrogation By Docosahexaenoic Acid, Nuzhath Tajuddin, Kwan-Hoon Moon, Simon Alex Marshall, Kimberly Nixon, Edward J. Neafsey, Hee-Yong Kim, Michael A. Collins

Pharmaceutical Sciences Faculty Publications

Evidence that brain edema and aquaporin-4 (AQP4) water channels have roles in experimental binge ethanol-induced neurodegeneration has stimulated interest in swelling/edema-linked neuroinflammatory pathways leading to oxidative stress. We report here that neurotoxic binge ethanol exposure produces comparable significant effects in vivo and in vitro on adult rat brain levels of AQP4 as well as neuroinflammation-linked enzymes: key phospholipase A2 (PLA2) family members and poly (ADP-ribose) polymerase-1 (PARP-1). In adult male rats, repetitive ethanol intoxication (3 gavages/d for 4 d, ∼ 9 g/kg/d, achieving blood ethanol levels ∼ 375 mg/dl; "Majchrowicz" model) significantly increased AQP4, Ca+2-dependent PLA2 GIVA (cPLA2), phospho-cPLA2 GIVA …

Method Of Using Ccr3 Binding Agents To Detect Choroidal Neovascularization, Jayakrishna Ambati, Mark E. Kleinman

Ophthalmology and Visual Science Faculty Patents

The results presented herein demonstrate the specific expression of CCR3 in CNV endothelial cells in humans with AMD, and despite the expression of its ligands, eotaxin-1, -2, and -3, neither eosinophils nor mast cells are present in human CNV. The genetic or pharmacological targeting of CCR3 or eotaxins as disclosed herein inhibited injury-induced CNV in mice. CNV suppression by CCR3 blockade was due to direct inhibition of endothelial cell proliferation, and was uncoupled from inflammation as it occurred in mice lacking eosinophils or mast cells and was independent of macrophage and neutrophil recruitment. CCR3 blockade was more effective at reducing …

Mentoring High School Students, Robin L. Cooper

Kaleidoscope

Dr. Robin Cooper is a faculty member in the Department of Biology and teaches physiology and neurobiology courses at the University of Kentucky. His research is in comparative physiology. He received a double major with a B.S. in Chemistry and Zoology from Texas Tech in 1983 and a PhD in Physiology from Texas Tech Medical School in 1989. He has had postdoctoral training in Switzerland and Canada for 7 years before joining the University of Kentucky in 1996.

Democratizing The Food System- The Food Policy Council Movement, Garrett Spear

Kaleidoscope

The increasing complexity of our modern food system has changed the landscape of American agriculture and diet, and has increasingly consolidated decision-making abilities in a concentrated group of organizations. Local food movements are re-appropriating their food supply chains through a variety of methods, including the democratic-minded food policy council organization. Food policy councils organize community stakeholders into a network of organizations which can collectively grapple with food issues affecting and on behalf of the local community. This study consisted of research on the history and methods of the food policy council movement, directed ultimately at the council-less context in Lexington …

Satire And Stoicism: Pieter Bruegel The Elder's Triumph Of Death, Susan K. Gisselberg

Kaleidoscope

In Bruegel and the Creative Process, 1559 – 1563, Margaret Sullivan explains how the religious and political disorder of the Reformation in the Netherlands influenced Pieter Bruegel’s most original works, including The Triumph of Death. During this period, Bruegel combined classical elements and vernacular traditions. As a result of this process, he was able to depict similar imagery to his contemporaries, yet convey a vastly different concept. In a review, Todd Richardson argued that her claim relied heavily on classical literary sources with inadequate visual evidence in the work itself, and her correlations to antiquity rely solely on the …

Choosing My Avatar & The Psychology Of Virtual Worlds: What Matters?, Jennifer Wu

Kaleidoscope

Avatars and virtual worlds have become commonplace across the Internet in recent years through the development of the gaming industry and social media technology. The technology involved in virtual environments is becoming more accessible to the general public, and software for creating avatars or participating in virtual worlds can be found free online. Virtual worlds are used not only for recreation, but are also increasingly used for other purposes, such as education, marketing, and meeting places. We are finding new ways to represent ourselves online for various purposes. Recent research in psychology has shown that social phenomena in virtual worlds …

Effects Of Intraguild Cues Of Ground-Dwelling And Foliage-Dwelling Spiders On Lady Beetle Oviposition And Aphid Suppression, Rebecca Wente

Kaleidoscope

No abstract provided.

Can Alpha 7 Nicotinic Agonists Reduce The Effects Of 3rd Trimester Ethanol Exposure?, Victoria Votaw

Kaleidoscope

No abstract provided.

Empowering The Oppressed In 20th Century Literature Through Magical Realism, Lindsay Oberhausen

Kaleidoscope

No abstract provided.

Fiber Type Transition In Eccentric Exercised Normal Vs. Stretch-Activated Channel Blocked New Zealand Rabbits, Ryan Decoster

Kaleidoscope

No abstract provided.

Investigating The Structure And Function Of A Phosphatase Mutated In Lafora Disease, Kathryn Brewer

Kaleidoscope

No abstract provided.

The Sum Of Its Parts: Exploring The Motivations, Actions, And Social Realities Amongst Individuals Living Green And Ethical Lifestyles In Lexington, Ky, Sara Ailshire

Kaleidoscope

No abstract provided.