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Physiological Ecology Of Overwintering And Cold-Adapted Arthropods, Leslie Jean Potts Jan 2020

Physiological Ecology Of Overwintering And Cold-Adapted Arthropods, Leslie Jean Potts

Theses and Dissertations--Entomology

Given their abundance and diversity, arthropods are an excellent system to investigate biological responses to winter. Winter conditions are being majorly impacted by climate change, and therefore understanding the overwintering biology of arthropods is critical for predicting ecological responses to climate change. In Chapters 2 and 3, I investigate the winter biology of a winter-active wolf spider. I show that winter-active spiders can take advantage of periodic prey resources and grown in the winter, which may allow them to get a jumpstart on spring reproduction. I also investigate spiders’ ability to track changes in their environment by quantifying low temperature …


Emerald Ash Borer Development Across A Latitudinal Gradient: Implications For Biocontrol, Sarah Pellecchia Jan 2020

Emerald Ash Borer Development Across A Latitudinal Gradient: Implications For Biocontrol, Sarah Pellecchia

Theses and Dissertations--Entomology

Emerald ash borer, EAB, (Agrilus planipennis Fairmaire) is an invasive beetle that kills ash trees. It was accidentally introduced from China, and has rapidly expanded across North America, now occupying much of the eastern US. Four classical biocontrol parasitoids have been released to help mitigate its spread and impact: Tetrastichus planipennisi Yang, Spathius agrili Yang, S. galinae Belokobylskij and Strazanac, and Oobius agrili Zhang and Huang. These parasitoids have been deployed throughout EAB’s invaded range, but there has been limited recovery of the parasitoids from their release sites in southern states. I evaluated whether this lack of establishment might …


Management Of Stink Bugs (Hemiptera: Pentatomidae) On Soybean In Kentucky, Yaziri Gonzalez Jan 2020

Management Of Stink Bugs (Hemiptera: Pentatomidae) On Soybean In Kentucky, Yaziri Gonzalez

Theses and Dissertations--Entomology

Phytophagous stink bugs (Hemiptera: Pentatomidae) are pests of significant importance to soybean, Glycine max (L.), because they can cause severe losses in seed quality, ultimately affecting yield. Damage to soybean is caused through a process called extra-oral digestion. This occurs when the adults and nymphs of stink bugs pierce plant tissues with their mandibular and maxillary stylets, insert digestive enzymes, break down tissue, and extract the digested plant fluid. Three endemic stink bug species of North America are present in Kentucky: green stink bug (Chinavia hilaris), brown stink bugs (Euschistus species complex), and red-shouldered stink bug (Thyanta custator) …


An Educational Pilot Intervention To Increase Environmental Health Literacy Among Rural Kentucky Youth Attending Summer Camp, Ashley Rose Mattingly Jan 2020

An Educational Pilot Intervention To Increase Environmental Health Literacy Among Rural Kentucky Youth Attending Summer Camp, Ashley Rose Mattingly

Theses and Dissertations--Nutrition and Food Systems

Risk factors, including exposure to environmental pollutants, poor dietary and overall health outcomes, and low environmental health literacy (EHL), place the rural Appalachian youth population at increased risk for chronic diseases. Implementation of nutrition and pollution curriculum into two week-long summer day camps for youth ages 5-12 years that focus around healthy lifestyles and environmental stewardship has the potential to increase health protective action in this community. This research utilized qualitative interviews of 30 Kids on the Creek Camp attendees to determine the efficacy of the implementation of an active learning lesson to increase EHL by explaining the link between …


Cardiac Effects Of Obesity During Pregnancy In C57bl/6j Mice, Kayla Lynn Dudick Jan 2020

Cardiac Effects Of Obesity During Pregnancy In C57bl/6j Mice, Kayla Lynn Dudick

Theses and Dissertations--Nutrition and Food Systems

Objective: Pregnancy requires profound cardiac and metabolic adaptation. Left ventricular (LV) mass is increased in response to pregnancy, but is not associated with cardiac damage. In contrast, obesity-mediated cardiac hypertrophy is pathological. Data from animal studies indicate dietary fatty acid composition may have a protective effect during states of extreme cardiac physiological adaptation. In contrast, aberrant cardiac metabolism is a hallmark of disease. Over a third of reproductive-age women in the United States are obese, but there is a paucity of data describing the effect of obesity on maternal cardiac adaptation to pregnancy. The objective of this study was to …


Performance Evaluation And Community Needs Of Spay Our Strays Organization, Elizabeth Adams Jan 2020

Performance Evaluation And Community Needs Of Spay Our Strays Organization, Elizabeth Adams

MPA/MPP/MPFM Capstone Projects

Free-roaming cat populations have been linked to public health concerns and negative impacts on animal shelter resources. Trap-neuter-return has gained popularity in the last few decades as a humane and effective approach to decrease the population of community cats and address these concerns. Spay Our Strays advocates for trap-neuter-return practices in Lexington, Kentucky, by providing services to the local community and surrounding counties. Strategic planning techniques and comparative analysis are used to develop recommended strategic actions the organization can use to improve their impact. Findings showed a need to invest in board development, diversify funding sources, focus on community-level targeting, …


Improving Kentucky's Winter Wheat And Double Crop Soybean Rotation, Katherine Susan Rod Jan 2020

Improving Kentucky's Winter Wheat And Double Crop Soybean Rotation, Katherine Susan Rod

Theses and Dissertations--Plant and Soil Sciences

The winter wheat double crop soybean rotation is an economically viable rotation for Kentucky farmers. Recent decreases in commodity prices has warranted the need to evaluate intensive management practices that can increase yields and profitability in this crop rotation. There were three goals of this dissertation: 1.) identify management practices that would decrease deoxynivalenol (DON) in harvested wheat grain and increase wheat heading and anthesis uniformity, 2.) evaluate double crop soybean planting timing and identify intensive management practices to increase seed yield, and 3.) determine the profitability of these management options. These studies were conducted in Princeton KY between 2016 …


Glyphosate Plus Dicamba Spray Solution Deposition, Coverage, And Efficacy As Influenced By Spray Nozzle Design And Weed Density, Madison Dru Kramer Jan 2020

Glyphosate Plus Dicamba Spray Solution Deposition, Coverage, And Efficacy As Influenced By Spray Nozzle Design And Weed Density, Madison Dru Kramer

Theses and Dissertations--Plant and Soil Sciences

Dicamba injury to sensitive soybean and other broadleaf crops due to drift is a major issue and label restrictions have been created to mitigate dicamba drift. One restriction is the mandated use of low drift nozzles to spray dicamba; these nozzles produce larger droplets and minimize the production of driftable fines. Experiments were conducted to evaluate herbicide coverage, deposition, and efficacy. Three spray nozzle designs and different weed densities were the main factors in the analysis. Dicamba plus glyphosate was applied to 5 to 10 cm tall weeds with a Turbo TeeJet (TT11005) nozzle and two drift reduction nozzles approved …


Modulation Of Epileptogenesis: A Paradigm For The Integration Of Enzyme-Based Microelectrode Arrays And Optogenetics, Corwin R. Butler, Jeffery A. Boychuk, Francois Pomerleau, Ramona Alcala, Peter Huettl, Yi Ai, Johan Jakobsson, Sidney W. Whiteheart, Greg A. Gerhardt, Bret N. Smith, John T. Slevin Jan 2020

Modulation Of Epileptogenesis: A Paradigm For The Integration Of Enzyme-Based Microelectrode Arrays And Optogenetics, Corwin R. Butler, Jeffery A. Boychuk, Francois Pomerleau, Ramona Alcala, Peter Huettl, Yi Ai, Johan Jakobsson, Sidney W. Whiteheart, Greg A. Gerhardt, Bret N. Smith, John T. Slevin

Physiology Faculty Publications

BACKGROUND: Genesis of acquired epilepsy includes transformations spanning genetic-to- network-level modifications, disrupting the regional excitatory/inhibitory balance. Methodology concurrently tracking changes at multiple levels is lacking. Here, viral vectors are used to differentially express two opsin proteins in neuronal populations within dentate gyrus (DG) of hippocampus. When activated, these opsins induced excitatory or inhibitory neural output that differentially affected neural networks and epileptogenesis. In vivo measures included behavioral observation coupled to real-time measures of regional glutamate flux using ceramic-based amperometric microelectrode arrays (MEAs).

RESULTS: Using MEA technology, phasic increases of extracellular glutamate were recorded immediately upon application of blue light/488 nm …


Terminal Youth: The Failure Narrative Of The Dysfunctional Family As The Non-Viability Of Capitalist Economic Liberalism In Contemporary Latin American Film, Sharrah Lane Jan 2020

Terminal Youth: The Failure Narrative Of The Dysfunctional Family As The Non-Viability Of Capitalist Economic Liberalism In Contemporary Latin American Film, Sharrah Lane

Theses and Dissertations--Hispanic Studies

This project examines the desire for national and international belonging and citizenship in the figure of the child intersectionally marked by race, class, and gender in contemporary Latin American film, a desire that is ultimately met only with precarity and violence. Chapter One analyzes the figure of the orphaned street child in terms of the desire for connection with a mother figure as a stand-in for the lack of affective community in Pixote: a lei do mais fraco (Brazil, 1981), La vendedora de rosas (Colombia, 1998), and Huelepega: ley de la calle (Venezuela, 1999) in which the protagonists either die, …


Poverty And Nonprofits: Investigating The Relationship Between Poverty And Nonprofit Activity In The United States, Megan Combs Jan 2020

Poverty And Nonprofits: Investigating The Relationship Between Poverty And Nonprofit Activity In The United States, Megan Combs

MPA/MPP/MPFM Capstone Projects

In order to better understand our society and institutions, we can look at the relationships between institutions, social infrastructure, and human conditions. Social and institutional networks can influence the flow of resources on a scale that individuals cannot. These structures can perpetuate or alleviate social and human conditions but, alternatively, social conditions can mobilize and influence institutional structures. As systemic poverty remains a social issue, various US programs and forms of aid continue to be delivered despite limiting and varying levels of success. One such mode of aid is provided by nonprofits. Studies have shown that nonprofits empirically do very …


Cost-Benefit Analysis Of Composting At The University Of Kentucky, Jennifer Sutton Jan 2020

Cost-Benefit Analysis Of Composting At The University Of Kentucky, Jennifer Sutton

MPA/MPP/MPFM Capstone Projects

This study is a cost-benefit analysis (CBA) of the University of Kentucky’s composting program. Food waste is collected from two major dining facilities on campus and composted at the university’s C. Oran Little Research Center in Woodford County. This CBA only considers the direct accounting costs of the program. Three models were developed and analyzed. Ultimately, all three models resulted in a deficit over the life of the project. However, it is important to note that benefits were likely understated because this CBA did not take into consideration the environmental or social benefits that exist from a composting program at …


Disassemble/Analyze/Assemble: How A Hands-On Engineering Project Affects High School Girls' Science/Engineering Self-Efficacy, Interest And Career Considerations, Jennifer Corbett Ferguson Jan 2020

Disassemble/Analyze/Assemble: How A Hands-On Engineering Project Affects High School Girls' Science/Engineering Self-Efficacy, Interest And Career Considerations, Jennifer Corbett Ferguson

Theses and Dissertations--Education Sciences

Engineering education as part of the K-12 curriculum can be an effective instructional tool and its benefits include improved science and mathematical achievement as well as an increased interest and understanding of the engineering field, especially for female students. However, there is a serious lack of research-based engineering curriculum being used at the middle and high school levels and lessons most often rely on building or construction competitions. Over the past decade or two, many well-known colleges have implemented a reverse engineering instructional unit known as Disassemble/Analyze/Assemble projects within their introductory engineering courses. These units have been shown to improve …


The Agricorps Experience: A Phenomenology Of International Agricultural Educators, Kendall Mallory Wright Jan 2020

The Agricorps Experience: A Phenomenology Of International Agricultural Educators, Kendall Mallory Wright

Theses and Dissertations--Education Sciences

With the growing world population, agricultural education may play a role in increasing the production of agricultural products and services in developing nations by improving knowledge and participation in the agricultural field, specifically with regard to the nations’ youth. Few researchers have sought to understand how the experience of teaching agriculture abroad impacts the agricultural educator. The purpose of this dissertation was to understand the essence of an international agricultural educator experience. In this phenomenological study, I interviewed past AgriCorps fellows to examine the lived experiences of fellows, how the fellows were impacted personally and professionally, and the transformative learning …


Muslim College Students And Civic Engagement, Aus Abdulwahhab Jan 2020

Muslim College Students And Civic Engagement, Aus Abdulwahhab

Theses and Dissertations--Educational Policy Studies and Evaluation

This study explores the nature of civic engagement of Muslim college students in the United States. The Community of Practice Theory is used as a conceptual structure to explore the relation between meaning, practice, identity and organization for Muslim students active in civic engagement through campus-based Muslim Student organizations. Pluralism, as shared values that combine different people and advocates wider civic participation, was adopted as part of the conceptual framework to see how the Muslim students think, serve, learn, and develop throughout the process of engagement on campus and in the local community. Particular attention is paid to the impacts …


Opportunity And Access To Informal Stem Learning Environments, Abigail Fowler, Caitlyn Yost, Margaret J. Mohr-Schroeder Jan 2020

Opportunity And Access To Informal Stem Learning Environments, Abigail Fowler, Caitlyn Yost, Margaret J. Mohr-Schroeder

Posters-at-the-Capitol Presentations

The University of Kentucky (UK) STEM Experiences is a collaboration amongst the UK Colleges of Education, Engineering, and Arts & Sciences. Our goal is to expose students to a variety positive learning experiences and career options in the STEM fields. Additionally, the summer experiences.


Differential Effects Of Digital Vs. Print Text On Reading Comprehension And Behaviors In Students With Adhd, Lara Nash Campbell Jan 2020

Differential Effects Of Digital Vs. Print Text On Reading Comprehension And Behaviors In Students With Adhd, Lara Nash Campbell

Theses and Dissertations--Curriculum and Instruction

This study was conducted using a qualitative single-case, embedded design in a 4th grade elementary classroom with five participants. One purpose of this inquiry was to evaluate the differential effects between digital and print text on reading comprehension of literary texts on students with ADHD. A second purpose of this study was to evaluate and determine the differential effects reading digitally and from print have on specific off and on-task reading behaviors with these same participants. The goal of this research was to generate a comprehensive case description and analysis that will contribute to the evolving theories of best practice …


Assessing Learning Efficiency In Narrative Simulation Delivered Through Interactive Multimedia, Christopher Shannon Daniel Jan 2020

Assessing Learning Efficiency In Narrative Simulation Delivered Through Interactive Multimedia, Christopher Shannon Daniel

Theses and Dissertations--Curriculum and Instruction

This study evaluated the effects of Narrative Simulation (NS) on learning and cognitive load. Specifically, it measured the potential differences in observed instructional efficiency when comparing a self-paced expository multimedia lesson to a NS lesson which involves a character-focused story with multiple decision inputs at key points.

This ex post facto design observed 119 participants consisting of preservice teachers from a large public university in the southeastern United States. They were divided into two sequence groups: (a) Expository Lesson Group; and (b) Narrative Simulation group. The Expository group received Expository Lesson One first, then Expository Lesson Two, and then Narrative …


Compositional Modeling: A Classical Imitative Pedagogy For The Modern Era, Robert Harris Jeter Jan 2020

Compositional Modeling: A Classical Imitative Pedagogy For The Modern Era, Robert Harris Jeter

Theses and Dissertations--Music

Students of composition develop two skills simultaneously: craft and creativity. Since students are more naturally inclined to focus on one of these skills over the other, finding the proper balance is an educational challenge. An effective pedagogical system for composition cultivates both of these necessary skills.

Imitation was a preferred pedagogical approach during the Classical period. In his 1848 book School of Practical Composition, Carl Czerny instructs his readers to apply the extensive rules of composition by strictly following a compositional model. Although this methodology lost favor during the nineteenth century, the imitative techniques presented by Czerny are flexible enough …


Passacaglia And Its Related Forms: Before, During, And After 18th Century Baroque And Program Notes, Austin Han Jan 2020

Passacaglia And Its Related Forms: Before, During, And After 18th Century Baroque And Program Notes, Austin Han

Theses and Dissertations--Music

The purpose of this project is to inform readers of the importance musicology has in informing one’s own learning experience as a performer and listener. As an expression of this exercise, I’ve focused on the history of the passacaglia in Europe from its origins in Spain to its culmination in the music of Paul Hindemith (1895-1963). Through the process of learning about different renditions of passacaglia through musical analysis and comparisons with contemporary art movements and their legacies, I hope the results of what are shown displays an urgent appreciation for applying a more nuanced and openminded approach to the …


Zappa’S Remembrance Of A Forgotten Style: The Relationship Between Frank Zappa And Doo-Wop As Illustrated In Cruising With Ruben & The Jets, Benjamin Adamo Jan 2020

Zappa’S Remembrance Of A Forgotten Style: The Relationship Between Frank Zappa And Doo-Wop As Illustrated In Cruising With Ruben & The Jets, Benjamin Adamo

Theses and Dissertations--Music

One of Zappa’s many cherished styles of music was the doo-wop of the mid-1950s through the early 1960s. His love for the style culminated in 1968 with a poor-selling and often forgotten doo-wop record, Cruising with Ruben & The Jets. While the record earned little praise from critics and fans, Zappa considered it as a landmark of his early career, as evidenced in his autobiography. This thesis investigates Zappa’s relationship to doo-wop and “perversion” of the style as it culminated in Cruising.

As revealed through autobiographical entries and interviews with Zappa, Ray Collins, and other collaborators, Zappa devised …


Wholeness And Belonging In Nikky Finney's Head Off & Split: An Eco-Politics Of Resilience And Resistance, Mary Rudolph Jan 2020

Wholeness And Belonging In Nikky Finney's Head Off & Split: An Eco-Politics Of Resilience And Resistance, Mary Rudolph

Theses and Dissertations--English

Nikky Finney’s Head Off & Split illuminates an urgent and radical eco-political project: the creation of whole, resilient, co-species communities capable of surviving interlocking political, social, and ecological crises. Finney foregrounds the strategic practice of belonging as a method of survival within contexts of systemic oppression and climate chaos. “Belonging,” in these terms, is not a “natural” ontological state, but a mode of co-being that is continually (re)created and (re)enacted through daily world-making practices: foodways, spatial habitation, migration and movement. Belonging is a collection of reciprocal, adaptive, situated praxes that make and sustain beings and worlds. They rely on and …


Transformative Subjects: American Children’S Periodicals, 1855-1905, Emily R. Dehaven Jan 2020

Transformative Subjects: American Children’S Periodicals, 1855-1905, Emily R. Dehaven

Theses and Dissertations--English

Through this dissertation, I explore the ways that authors and editors use the form of children’s periodicals to discuss questions of childhood independence, the structure of the family, and the balance of power between children and adults with regards to literary texts. I examine the ways in which adults and children negotiate control over the periodicals and over the images of transformation present within those texts. Periodicals offer a unique opportunity for interaction between readers and editors. Magazines and newspapers encouraged readers to write into the magazine to offer their own insights and opinions. Readers of children’s magazines even had …


Curious Natures: Constructing Queer Ecologies In Early America, Richard Lee Parmer Jr. Jan 2020

Curious Natures: Constructing Queer Ecologies In Early America, Richard Lee Parmer Jr.

Theses and Dissertations--English

This dissertation argues that early American writers often constructed queer ecologies in order to naturalize Anglo-American civilization and justify its expansion into Native American territories. Since there is so little textual evidence on the subject, the major challenge to studying sexuality in early America is approaching sexuality studies creatively—to broaden both our understanding of what counts as sexual discourse and our frameworks for analyzing it. My dissertation addresses this challenge through what many ecocritical scholars of sexuality call queer ecology. In their groundbreaking anthology on the topic, Catriona Mortimer-Sandilands and Bruce Erikson remind us that, historically and in the present, …


“We Had Become Trailer People”: Stigma, Social Boundary Making, And The Story Of The American Mobile Home Park, Katie M. Founds Jan 2020

“We Had Become Trailer People”: Stigma, Social Boundary Making, And The Story Of The American Mobile Home Park, Katie M. Founds

Theses and Dissertations--Sociology

Mobile homes and mobile home parks—still most often called trailer parks in common vernacular—occupy a particularly stigmatized position in American culture. A symbolic stand-in for a host of social ills, from bad hygiene and broken families to drug use and loose morals, mobile homes offer affordable housing at a social cost, branding their residents as likewise deficient. This piece of material culture did not come into being with such negative meanings attached. The process of becoming a symbol of stigma is an historical one, a story of meaning making in the midst of cultural shifts and changing norms. Since appearing …


Pneumovirus Infections: Understanding Rsv And Hmpv Entry, Replication, And Spread, Jonathan T. Kinder Jan 2020

Pneumovirus Infections: Understanding Rsv And Hmpv Entry, Replication, And Spread, Jonathan T. Kinder

Theses and Dissertations--Molecular and Cellular Biochemistry

Pneumoviruses including human metapneumovirus (HMPV) and respiratory syncytial virus (RSV) are significant causes of respiratory tract infections globally. Children, elderly, and immunocompromised patients are at the greatest risk for developing severe infections, which can have devastating outcomes. Although these viruses are ubiquitous with significant impacts on human health, there are no antivirals or vaccines available. The only FDA approved therapy is a monoclonal antibody for RSV, given prophylactically during the infectious season, and this treatment is only available for high risk infants. The work presented in this thesis aims to increase our understanding of how these viruses enter, replicate, and …


From Organisms To Ecosystems: Impacts Of Limb Loss And Regeneration On Crayfish Behavior, Luc Arnaud Dunoyer Jan 2020

From Organisms To Ecosystems: Impacts Of Limb Loss And Regeneration On Crayfish Behavior, Luc Arnaud Dunoyer

Theses and Dissertations--Biology

The ability of some organisms to regenerate tissues and organs has fascinated naturalists since antiquity, dating back to the earliest accounts of scientific inquiry with Aristotle in Ancient Greece. Then, Darwin’s theory invigorated some scientists’ dream of stimulating (or reactivating) regenerative capacities in human beings by showing that we are related to highly regenerative organisms. More recently, a renewed interest in discovering the molecular and genetic basis for organ and tissue regeneration has led biologists to focus more specifically on a restrictive set of model organisms.

Although the process of limb regeneration is different between invertebrate and vertebrate organisms, it …


Elucidating The Role Of Light-Induced Circadian Disruption On Atherosclerosis In Apolipoproteine-Deficient Mice, Jeffrey Chalfant Jan 2020

Elucidating The Role Of Light-Induced Circadian Disruption On Atherosclerosis In Apolipoproteine-Deficient Mice, Jeffrey Chalfant

Theses and Dissertations--Biology

Circadian rhythms are approximately 24-hour oscillations of nearly every biological process in the body. The circadian system coordinates these rhythms of physiology and behavior with environmental cycles such as the light-dark cycle. Shift workers, who experience irregular exposure to the light-dark cycle, have chronically disrupted circadian rhythms and increased risk of developing cardiovascular disease, but the mechanisms are unknown. Our studies investigated the effects of light-induced circadian disruption on atherosclerosis in ApolipoproteinE-deficient (ApoE-/-) mice. We found that male ApoE-/- mice housed in constant light for 12 weeks, which results in severe disruption of circadian rhythms …


The Roles Of Polar Cell Extensions In Drosophila Micropyle Formation, Bradford Hull Jan 2020

The Roles Of Polar Cell Extensions In Drosophila Micropyle Formation, Bradford Hull

Theses and Dissertations--Biology

The Drosophila micropyle is a conserved formation utilized to allow sperm passage past the robust eggshell structure for fertilization. Micropyle formation follows a unique acellular tubulogenesis method where it is secreted and shaped by specialized follicle cells including the border cells and polar cells. In late oogenesis, the polar cells form extensions that are necessary to create the micropyle pore through which sperm enters. Previous work established that polar cell extension presence is required for micropyle pore formation. We investigated temporal requirements of extensions throughout chorion deposition and found extensions are required during the beginning and middle of choriogenesis, but …


Evaluation Of The Influence Of Dicamba Exposure On Crop Injury And Canopy Closure Of Glufosinate Resistant Soybean, Zachary K. Perry Jan 2020

Evaluation Of The Influence Of Dicamba Exposure On Crop Injury And Canopy Closure Of Glufosinate Resistant Soybean, Zachary K. Perry

Theses and Dissertations--Plant and Soil Sciences

Dicamba-resistant soybean along with lower volatility dicamba formulations have been introduced in an attempt to control herbicide resistant weeds such as Amaranthus palmeri. This introduction has increased the amount of dicamba being applied later in the growing season increasing the prevalence of dicamba off-target movement. Dicamba damage was simulated by applying low rates of dicamba directly on soybeans at rates (0.5 g ae ha-1, 1 g ae ha-1 and 5 g ae ha-1 dicamba) and five-exposure timings from June 1 to July 10. The experimental design was a randomized complete block with four replications at …