Digital Commons Network^™

Geologic Maps And Geologic Issues In Kentucky: A Citizen's Guide, Carol Ruthven, John D. Kiefer, Stephen F. Greb, William M. Andrews Jr.

Special Publication--KGS

Citizens in all walks of life benefit from the information on geologic maps. Geologic maps are used to:

• Identify areas prone to natural hazards, such as landslides, erosion, sinkholes, and flooding
• Select locations that are suitable for tunnels, bridges, dams, and quarries
• Identify suitable sites for safe construction of homes and commercial buildings
• Select safe places for building septic systems and landfills to minimize the risk of polluting soils and groundwater
• Help farmers, landowners, and government officials locate groundwater resources and protect water quality
• Help citizens understand geologic features (such as faults, mineral deposits, and impact structures) in the landscape …

Evaluation Of Flow Dynamics Through An Adjustable Systemic-Pulmonary Artery Shunt, Timothy Brown

University of Kentucky Master's Theses

An adjustable systemic-pulmonary artery (SPA) shunt is being developed that consists of apolytetrafluoroethylene (PTFE) graft with a screw plunger mechanism. This device would allowfull control of flow through SPA shunts used to augment pulmonary blood flow in neonates bornwith single ventricle physiology. The objective of this study is to evaluate the changes this mechanismhas on flow fields for a 4 mm and 5 mm adjustable SPA shunt. Two in vitro models wereexamined; an idealized model with an axisymmetric constriction and a model developed from 3-Dreconstruction of the actual shunt under asymmetric constriction. These models were used to measurethe instantaneous velocity …

Modification Of The Nucleotide Cofactor-Binding Site Of Cytochrome P450 Reductase To Enhance Turnover With Nadh In Vivo, Calvin Lee Elmore

University of Kentucky Doctoral Dissertations

NADPH-cytochrome P450 reductase is the electron transfer partner for the cytochromes P450, heme oxygenase, and squalene monooxygenase, and is a component of the nitric oxide synthases and methionine synthase reductase. P450 reductase shows very high selectivity for NADPH and uses NADH only poorly. Substitution of tryptophan 677 with alanine (W677A) has been shown by others to yield a 3-fold increase in turnover with NADH, but profound inhibition by NADP+ makes the enzyme unsuitable for in vivo applications. In the present study site-directed mutagenesis of amino acids in the 2'-phosphate-binding site of the NADPH domain, coupled with the W677A substitution, was …

Entrainment Of Electrical Activation By Spatio-Temporal Distributed Pacing During Ventricular Fibrillation, Yiping Gu

University of Kentucky Master's Theses

Spatio-temporal variation in action intervals during ventricular fibrillation (VF) suggestthat the excitable gap may also be distributed spatio-temporally. The observation leadus to hypothesize that distributed pacing can be used to modify and entrain electricalactivation during VF. We tested this hypothesis using simulated VF and animal studies.

We simulated VF in a 400 by 400 cell matrix. Simulation results showed that activationpattern could be entrained using spatially distributed stimulation. Up to a certain limit,increasing stimulus strength and density led to improved entrainment. Best entrainmentwas obtained by pacing at a cycle length similar to the intrinsic cycle length.

In order to verify …

Experimental Investigation Of Separation In A Low Pressure Turbine Blade Cascade Model, Brian Hollon

University of Kentucky Master's Theses

The flow field around a low pressure turbine blade is examined using smoke-wire flow visualization, static surface pressure measurements, and particle image velocimetry (PIV). The purpose of the experimental study is to investigate the transition and separation characteristics on low pressure turbine blades under low Reynolds number (Re) and varying freestream turbulence intensity (FSTI). A cascade model consisting of 6 Pratt andamp; Whitney PAK-B low pressure turbine blades was examined in a wind tunnel using PIV and flow visualization. Smoke-wire visualization was performed for test section exit angles of 93°, 95°, and 97°, in the range Re = …

At The Intersection Of Proximate Cause And Terrorism: A Contextual Analysis Of The (Proposed) Restatement Third Of Torts' Approach To Intervening And Superseding Causes, Jim Gash

Kentucky Law Journal

No abstract provided.

"Don't Show Them Where To Click And Vote:" An Assessment Of Electioneering Law In The United States As A Consideration In Implementing Internet Voting Regimes, Michael Odell Walker

Kentucky Law Journal

No abstract provided.

An Enduring Fear: Recent Limitations On The Past Persecution Ground For Asylum, Susannah C. Vance

Kentucky Law Journal

No abstract provided.

Toward A Coherent Approach To Tort Immunity In Judicially Mandated Family Court Services, Nat Stern, Karen Oehme

Kentucky Law Journal

No abstract provided.

Psychological And Sleep Quality Differences Between Chronic Daily Headache And Temporomandibular Disorders Patients, Eduardo B. Vazquez

University of Kentucky Master's Theses

The aim of this study was to investigate whether chronic daily headache (CDH) and temporomandibular disorders (TMD) patients present with different psychological and sleep quality characteristics. Sixty seven patients diagnosed with CDH according to Silberstein et al.s classification criteria were matched by age, sex, pain intensity, and pain duration with 67 patients who had a primary diagnosis of myofascial pain (MP), and 67 patients with a primary diagnosis of TMJ intracapsular pain (IC) according to the Research Diagnostic Criteria for TMD. The CDH group was comprised of three mutually exclusive diagnostic groups, that is transformed migraine (n=35), chronic tension-type headache …

"Male Wealth" And "Claims To Motherhood": Gendered Resource Access And Intergenerational Relations In The Gwembe Valley, Zambia, Lisa Cliggett

Anthropology Faculty Publications

No abstract provided.

Chronic Ethanol Consumption Inhibits Multiple Apoptotic Pathways In The Rat Pancreatic Acinar Cell, Franco Fortunato

University of Kentucky Doctoral Dissertations

Multiple lines of experimental evidence demonstrate that chronic alcoholconsumption causes mitochondrial injury, as well as acinar cell oxidative and metabolicstress. Alcoholics are more susceptible to acute and chronic pancreatitis. Despite alcoholrelatedacinar cell injury, apoptosis, or programmed cell death, appears to be reduced inacinar cells rather than increased, as is seen in the liver.This work describes the possible mechanisms through which alcohol affects theacinar cell apoptosis pathways in the rat pancreas. Two apoptotic pathways wereinvestigated: (1) receptor-mediated apoptosis via Fas/FasL and caspase-8, and (2)mitochondrial-mediated apoptosis via Bcl-2/Bax and caspase-9. Both pathways canactivate the final apoptosis executer caspase-3. Using the Lieber-DeCarli alcohol/controldiet, …

Functional Magnetic Resonance Imaging Study Of Pain And Emotion, Claude Ervin Davis

University of Kentucky Doctoral Dissertations

Neuroscience research has followed two fairly distinct paths in investigating central neural mechanisms of pain and emotion. Rarely have studies been conducted which intentionally combined painful and emotional stimulation while observing brain function. Theories of emotion and pain processing predict an interaction between pain and emotion such that emotional states may serve to both increase or decrease pain. This increase or decrease may also correspond to different effects on different dimensions of the overall pain experience as defined in pain neuromatrix theory. Theories of emotion begin with emotions as interpretations of bodily states, to more contemporary theories focusing on the …

Cellular Trafficking Properties And Physiological Functions Of The Á1-Adrenoceptor Subtypes, Dan Chalothorn

University of Kentucky Doctoral Dissertations

The 1-adrenoceptors (1-ARs) serve as an interface between the sympathetic nervous system and the cardiovascular system where they are mediators of systemic arterial blood pressure, initiators of positive inotropy, and regulators of cellular growth responses. There are three subtypes: 1A-, 1B-, and 1D-ARs. This dissertation research investigated the trafficking properties of the 1-ARs at the cellular level as well as physiological relevance of the 1-ARs at the tissue level. In vitro studies using transiently transfected 1-AR/GFP subtypes revealed distinct basal localization patterns and different agonist-mediated activation and desensitization properties. The 1A- and the 1B-AR/GFP subtypes displayed agonist-mediated receptor redistribution, in …

Coevolution And Genetic Diversity In Grass-Endophyte Symbioses, Kelly D. Craven

University of Kentucky Doctoral Dissertations

Symbioses between cool-season grasses (Subfamily Pooideae) and endophytic fungi in the genera Epichlo and Neotyphodium straddle a continuum of interactions from antagonistic to highly mutualistic. Although these two genera of endophytes are closely related, Neotyphodium endophytes are strictly seed-transmitted and provide many physiological and defensive benefits to their hosts, while Epichlo spp. have an obligately sexual contagious stage wherein host inflorescences are replaced by fungal sexual structures (stromata), effectively sterilizing the plant. Between these two extremes of interactions are Epichlo spp. with a mixed strategy, where some grass tillers are sterilized while others develop normally and yield healthy endophyte-infected seeds. …

Soil And Colloidal Phosphorus Dynamics In Three Ky Soils: Bioavailability, Transport And Water Quality Implications, Konstantinos Christos Makris

University of Kentucky Master's Theses

Particulate P constitutes a significant portion of the total P found in surface runoff water. Water dispersed P-containing particles can travel long distances via surface runoff and reach water bodies causing decrease in water quality. The main objective of the study was to evaluate the potential facilitation of P transport by the water dispersed soil colloids (WDC) using three KY soils with a long-term record of poultry manure, and fertilizer P applications. Sequential fractionation for both whole soils and colloidal samples revealed that the WDC had a greater total and labile P content than the soil as a whole. Also, …

Comparative Gene Mapping For Equus Przewalskii And E. Hemionus Onager With Investigation Of A Homologous Chromosome Polymorphism In Equidae, Jennifer Leigh Myka

University of Kentucky Doctoral Dissertations

The ten extant species in the genus Equus are separated by less than 3.7 million years of evolution. Three lines of investigation were pursued to further characterize equid genome organization. 1.) The Przewalski.s wild horse (E. przewalskii, EPR) has a diploid chromosome number of 2n=66, while the domestic horse (E. caballus, ECA) has 2n=64. A comparative gene map for E. przewalskii was constructed using 46 bacterial artificial chromosome (BAC) probes previously mapped to 38 of 44 E. caballus chromosome arms and ECAX. BAC clones were hybridized to metaphase spreads of E. przewalskii and localized by fluorescent in situ hybridization (FISH). …

A Detailed Sector Analysis Of The Holstein Beef Market, Kenneth H. Burdine

University of Kentucky Master's Theses

The Holstein beef sector is a fascinating and integral part of the United States beefsystem; however, it has been largely overlooked in academic research. Holstein beef has longsuffered from perceptions that it is of poor quality. Recent changes in slaughter industrystructure, marketing systems, and production models have made the Holstein systemunbelievably complex. Coupled with econometric modeling, this sector analysis uses a semistructuredinterview approach to evaluate the reality of these perceptions, the impact of thesechanges, and to determine what truly drives the Holstein beef market. Results suggest that manyof the perceptions of Holstein beef are inaccurate; the market for Holstein steers …

Minimum Inhibitory Concentrations Of Two Common Food Phenolic Compounds And Their Effect On The Microbial Ecology Of Swine Feces In Vitro, Jennifer I. Zaffarano

University of Kentucky Master's Theses

Feeding sub-therapeutic levels of antibiotics to livestock has been associated withdevelopment and spread of antibiotic resistant bacteria. The present experiment was conductedto investigate the effect of antibiotic alternatives (caffeic acid, chlorogenic acid, and carbadox)on the microbial ecology of swine feces in vitro.Minimum inhibitory concentrations of caffeic and chlorogenic acids were determined forseveral pathogens using macrobroth and agar dilution techniques. Gram-negative bacteria werenot inhibited. Caffeic acid inhibited four Staphylococcus aureus strains at 200 ppm or less, andtwo Clostridium perfringens strains at 300 ppm. Chlorogenic acid inhibited all S. aureus strainsat 500 ppm, and one C. perfringens strain at 400 ppm.Effects of …

Development Agents And Nomadic Agency In The Damergou, Niger: Four Perspectives In The Development "Market", Karen Marie Greenough

University of Kentucky Master's Theses

Discourse between development agents and nomads implies that development for nomadiccommunities is a contradiction in terms. A "market-oriented" subtext underscores the investors'power of international agencies, the brokers' competition of intermediate organizations, and thenomadic clients' opportunism. Interviews and participant-observation focused on relationsbetween Wo??aa??e communities, bilateral and local organizations, and government extensionservices. I compare the data obtained with historical events to illuminate current phenomena.Approaches of structuration, political ecology, and disequilibrium theory aid an examination ofcommunication and discourses, and gaps in understanding between the parties. I find thatessential pastoral livelihood strategies of mobility and customary institutions are threatened bythe development system. How will …

Assessment Of Dietary Enzyme Supplementation On Ileal And Total Tract Digestibilities In Gestating And Lactating Swine, Ana Lúcia Pozzobon De Souza

University of Kentucky Doctoral Dissertations

The objective of this dissertation was to evaluate the effects of exogenous enzymesupplementation to gestation-lactation diets on the digestibility of nutrients in mature swinefemales and the associated implications on energy metabolism during gestation and lactationphysiologic states. Three experiments were performed to evaluate the effects of commerciallyavailable enzyme products or pure enzymes on the digestibility of nutrients. Crossover designs intwo periods during gestation (early and late gestation) and lactation were studied to obtain anincreased number of observations from the surgically cannulated sows.Experiment 1 evaluated the effects of two enzyme products supplemented to a practicalcorn soybean meal diet fed to second parity …

An Approach To The Measurement Of Sexual Behavior In The Bull (Bos Taurus) Using Variable Female Stimulus Conditions, John Denver Bailey

University of Kentucky Doctoral Dissertations

Most researchers studying sexual behavior of the bull have adopted the practice of severely restraining and sedating female stimuli, utilizing so-called "service stanchions" and quantifying behavioral events expressed by each bull. Based on these observations, bulls are classified as having high, medium, or low libido and ranked according to their ability to "service" the inanimate stimuli. It has been assumed that these methods represent appropriate measurements of reproductive behavior that will be expressed in a natural mating scenario and that bull sexual behavior can be studied in a manner that is independent of the context of the female. Hence, conventional …

Pre-Copulatory Sexual Cannibalism In Fishing Spiders: The Ecology Of An Extreme Sexual Conflict, J. Chadwick Johnson

University of Kentucky Doctoral Dissertations

Pre-copulatory sexual cannibalism (pre-SC), or predation of a potential mate before sperm transfer, provides an ideal model system for behavioral ecology's current focus on inter-sexual conflict. Studying the North American fishing spider (Dolomedes triton), I tested three female-benefit hypotheses for pre-SC: indirect benefits, direct benefits, and aggressive spillover. First, pre-SC may reflect a mating bias providing females with 'good-genes' benefits. By manipulating each female's options with regard to the most cited phenotypic advantage in male spiders, body size, I show that while females exhibit no bias in their attack tendency on males of different body sizes, large males mate significantly …

The Role Of P53 In Oxidative Stress And Polyglutamine Neurotoxicity, Jay C. Dunn

University of Kentucky Doctoral Dissertations

Polyglutamine expansion disorders are progressive neurodegenerative diseasesthat are caused by the pathological expansion of polyglutamine repeats. Huntington'sdisease (HD) is a polyglutamine disorder caused by the expansion of an existingpolyglutamine tract in a novel protein, Huntingtin (Htt). Oxidative stress has beenimplicated in the neural dysfunction observed in multiple neurodegenerative conditionsincluding HD. The tumor suppressor p53 is a multifunctional protein that has roles inthe cell cycle, apoptosis and neurodevelopment. The role of p53 in HD-associatedneurodegeneration has been studied but not fully elucidated, nor has the role of p53 inoxidative stress toxicity been fully elucidated.Here I present work that demonstrates polyglutamine expansion inducedalterations …

A Spatial Model For Evaluating Variable-Rate Fertilizer Application Accuracy, John Patrick Fulton

University of Kentucky Doctoral Dissertations

The popularity of variable-rate technology (VRT) has grown. However, the limitations and errors ofthis technology are generally unknown. Therefore, a spatial data model was developed to generate "asapplied"surfaces to advance precision agricultural (PA) practices. A test methodology based on ASAEStandard S341.2 was developed to perform uniform-rate (UR) and variable-rate (VR) tests to characterizedistribution patterns testing four VRT granular applicators (two spinner spreaders and two pneumaticapplicators). Single-pass UR patterns exhibited consistent shapes for three of the applicators with patternsshifts observed for the fourth applicator. Simulated overlap analysis showed that three of the applicatorsperformed satisfactorily with most CVs less than 20% while …

Inhibitory Properties Of Microplitis Croceipes Teratocyte Secretory Products And The Recombinant Protein Tsp14 On Protein Synthesis, Francis Anthony Diluna

University of Kentucky Master's Theses

Microplitis croceipes is a solitary endoparasitic wasp that oviposits in the hemocoel of Heleothis virescens larvae. Upon parasitization, the host larvaes physiology is altered; resulting in a compromised immune system and a decrease in the production of some vital proteins resulting in a terminal post-wandering prepupal state. Teratocytes, cells derived from the extraembryonic serosa of the parasitic wasp, mimic symptoms of parasitization when injected into host larvae, independent of other factors like polydnavirus and venom. Some of the inhibition of protein synthesis can be attributed to proteins secreted by the teratocytes (teratocyte secretory proteins or TSP). A fraction of TSP …

Elk Calf Survival, Mortality, And Neonatal Habitat Use In Eastern Kentucky, Nathan W. Seward

University of Kentucky Master's Theses

I estimated survival, cause-specific mortality, and neonatal habitat use of elk (Cervus elaphus nelsoni) calves in eastern Kentucky. I also measured habitat characteristics of elk parturition sites and annual calf production . Radio-collared females were fitted with vaginal implant transmitters and monitored for parturition behavior to locate, capture, and radio-collar calves during the springs of 2001 and 2002. Thirty-seven adult females with implant transmitters were translocated from Logan, Utah, to Addington Wildlife Management Area (WMA) in eastern Kentucky. Additional females from previous releases during 1997 and 1998 were monitored for parturition behavior. Mean calf production for all females monitored (n=77) …

Identification Of Natural Attenuation Of Trichloroethene And Technetium-99 Along Little Bayou Creek, Mccracken County, Kentucky, Abhijit Mukherjee

University of Kentucky Master's Theses

Natural attenuation of trichloroethene (TCE) and technetium (99Tc) was studied for five consecutive seasons (from January 2002 to January 2003) in Little Bayou Creek. The stream receives ground water discharge from an aquifer contaminated by past waste disposal activities at the Paducah Gaseous Diffusion Plant (PGDP), a uranium enrichment facility near Paducah, Kentucky. Results from stream gaging, contaminant monitoring, tracer tests (with bromide, nitrate, rhodamine WT and propane) and simulation modeling indicate the TCE is naturally attenuated by volatilization and dilution, with volatilization rates related to the ambient temperature and surface discharge rate. The only apparent mechanism of 99Tc attenuation …

Evaluation Of Fire-Fighting Helmet Surface Technology For High Radiant Heat Applications, David L. Barnett

University of Kentucky Master's Theses

Protective helmets used by fire-fighters must be designed to minimize the amount of heat transferred to the users head while providing durability, comfort, and affordable costs. This thesis highlights the evaluation of new helmet technology specifically tailored to high radiant heat environments to advance the state-of-the-art in head protection for this application. The research focused on the assessment of the outer shells of helmets and the properties of the surfaces. The development included the evaluation of radiation heat transfer, in a laboratory environment, to various helmet shell surface constructions. Industry standards were considered, and critiqued. Experiments were designed to isolate …

Pattern Evaluation For In-Plane Displacement Measurement Of Thin Films, Phanikrishna Thota

University of Kentucky Master's Theses

The term Gossamer is used to describe ultra-lightweight spacecraft structures that solve the aerospace challenge of obtaining maximum performance while reducing the launch costs of the spacecraft. Gossamer structures are extremely compliant, which complicates control design and ground testing in full scale. One approach is to design and construct smaller test articles and verify their computational models experimentally, so that similar computational models can be used to predict the dynamic performance of full-scale structures. Though measurement of both in-plane and out-of-plane displacements is required to characterize the dynamic response of the surface of these structures, this thesis lays the groundwork …