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Method For Treating A Viral Infection Related Or A Chemical Toxin Related Hepatic Injury With Deltorphin D, Peter R. Oeltgen, Paul D. Bishop, Craig J. Mcclain, Shirish Barve

Pathology and Laboratory Medicine Faculty Patents

A method of modulating cytokine mediated hepatic injury by administering compound-D SEQ ID NO:1 to a mammal. A concentration of the compound in the range of about 0.5 mg/kg to about 20 mg/kg in a physiologically acceptable formulation blocks a cytokine cascade. A therapeutic method of modulating cytokine mediated acute inflammatory, trauma induced and toxin induced hepatic injury, particularly via tumor necrosis factor modulation, is thus disclosed.

Alfalfa Hay And Balage: Testing For Quality, Kimberly Field

Kentucky Alfalfa and Stored Forage Conference

Why Test Your Hay?

• By knowing the percentages and protein levels you can increase/decrease the amount of supplements needed from your analysis report and will help the producer set feeding rations
• If you do not know what you have in your forage you are over or under feeding your livestock – therefore affecting the health of your animal and your pocket book

Alfalfa: Forage Crop Of The Future, Neal P. Martin, David Mertens, Ronald Hatfield, Hans Jung

Kentucky Alfalfa and Stored Forage Conference

In 2007, U.S. farmers harvested 23.6 million acres of alfalfa. Alfalfa harvested as hay and haylage produced 82.8 million tons valued at approximately $9.4 billion, ranking behind only corn and soybeans. Alfalfa hay supports dairy, beef, sheep, and horse production in the U.S. as well as a growing export market.

Haymaking: A Trip Down Memory Lane, Garry D. Lacefield, John E. Baylor

Kentucky Alfalfa and Stored Forage Conference

On a hot day in August, I climbed into the dust-free air-conditioned cab of a new tractor to assist in a hay baling demonstration during a field day. Hay yield was low as a result of the drought, so it was important that I get everything from the small windrow. I adjusted the volume on the surround sound stereo where I had just inserted a CD of the Eagles Greatest Hits. Now we’re ready to bale – first let me check the GPS and get a check on the moisture of the hay coming into the bale chamber – moisture …

Primers And Probe To Identify Mycobacterium Tuberculosis Complex, Subodh M. Lele, Manjiri S. Lele, Nada H. Khattar

Pathology and Laboratory Medicine Faculty Patents

Methods and nucleic acids for rapid, reliable and sensitive detection of Mycobacterium tuberculosis (MTB) complex pathogen in a biological sample. Oligonucleotides are provided which amplify MTB DNA and which are useful in carrying out real time PCR of DNA obtained from formalin-fixed and paraffin-embedded tissue samples.

Recycling Of Dolichyl Monophosphate To The Cytoplasmic Leaflet Of The Endoplasmic Reticulum After The Cleavage Of Dolichyl Pyrophosphate On The Lumenal Monolayer., Jeffrey S Rush, Ningguo Gao, Mark A Lehrman, Charles J Waechter

Molecular and Cellular Biochemistry Faculty Publications

During protein N-glycosylation, dolichyl pyrophosphate (Dol-P-P) is discharged in the lumenal monolayer of the endoplasmic reticulum (ER). Dol-P-P is then cleaved to Dol-P by Dol-P-P phosphatase (DPPase). Studies with the yeast mutant cwh8Delta, lacking DPPase activity, indicate that recycling of Dol-P produced by DPPase contributes significantly to the pool of Dol-P utilized for lipid intermediate biosynthesis on the cytoplasmic leaflet. Whether Dol-P formed in the lumen diffuses directly back to the cytoplasmic leaflet or is first dephosphorylated to dolichol has not been determined. Incubation of sealed ER vesicles from calf brain with acetyl-Asn-Tyr-Thr-NH(2), an N-glycosylatable peptide, to generate Dol-P-P in …

Effect Of Thyroid Hormone Concentration On The Transcriptional Response Underlying Induced Metamorphosis In The Mexican Axolotl (Ambystoma), Robert B. Page, Stephen R. Voss, Amy K. Samuels, Jeramiah J. Smith, Srikrishna Putta, Christopher K. Beachy

Biology Faculty Publications

BACKGROUND: Thyroid hormones (TH) induce gene expression programs that orchestrate amphibian metamorphosis. In contrast to anurans, many salamanders do not undergo metamorphosis in nature. However, they can be induced to undergo metamorphosis via exposure to thyroxine (T4). We induced metamorphosis in juvenile Mexican axolotls (Ambystoma mexicanum) using 5 and 50 nM T4, collected epidermal tissue from the head at four time points (Days 0, 2, 12, 28), and used microarray analysis to quantify mRNA abundances.

RESULTS: Individuals reared in the higher T4 concentration initiated morphological and transcriptional changes earlier and completed metamorphosis by Day 28. In contrast, initiation of metamorphosis …

Forage News [2008-02], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Kentucky Alfalfa Conference: 28 Years and Going Strong
• Forages at KCA
• AFGC/SRM Conference a Huge Success
• KY Producer and Students Win National Forage Awards
• Heart of America Grazing Conference
• Roundup Ready Alfalfa Update
• Can You Establish Clover and Suppress Winter Annual Weeds
• AVAIL Polymer Applied to Phosphorous Fertilizer
• Restricting Intake of Forages: An Alternative Feeding Strategy for Wintering Beef Cows
• Restricting Time of Access to Large Round Bales of Hay Affects Hay Waste and Cow Performance
• Upcoming Events

Thrombospondin-1 Is An Adipokine Associated With Obesity, Adipose Inflammation, And Insulin Resistance, Vijayalakshmi Varma, Aiwei Yao-Borengasser, Angela M. Bodles, Neda Rasouli, Bounleut Phanavanh, Greg T. Nolen, Emily M. Kern, Radhakrishnan Nagarajan, Horace J. Spencer, Mi-Jeong Lee, Susan K. Fried, Charlotte A. Peterson, Philip A. Kern

Clinical and Translational Science Faculty Publications

OBJECTIVE-We examined the relationship between the expression of thrombospondin (TSP)1, an antiangiogenic factor and regulator of transforming growth factor-β activity, obesity, adipose inflammation, and insulin resistance.

RESEARCH DESIGN AND METHODS-TSP1 gene expression was quantified in subcutaneous adipose tissue (SAT) of 86 nondiabetic subjects covering a wide range of BMI and insulin sensitivity, from visceral adipose (VAT) and SAT from 14 surgical patients and from 38 subjects with impaired glucose tolerance randomized to receive either pioglitazone or metformin for 10 weeks. An adipocyte culture system was also used to assess the effects of pioglitazone and coculture with macrophages on …

The Expression Of Microrna Mir-107 Decreases Early In Alzheimer's Disease And May Accelerate Disease Progression Through Regulation Of Β-Site Amyloid Precursor Protein-Cleaving Enzyme 1, Wang-Xia Wang, Bernard W. Rajeev, Arnold J. Stromberg, Na Ren, Guiliang Tang, Qingwei Huang, Isidore Rigoutsos, Peter T. Nelson

Sanders-Brown Center on Aging Faculty Publications

MicroRNAs (miRNAs) are small regulatory RNAs that participate in posttranscriptional gene regulation in a sequence-specific manner. However, little is understood about the role(s) of miRNAs in Alzheimer's disease (AD). We used miRNA expression microarrays on RNA extracted from human brain tissue from the University of Kentucky Alzheimer's Disease Center Brain Bank with near-optimal clinicopathological correlation. Cases were separated into four groups: elderly nondemented with negligible AD-type pathology, nondemented with incipient AD pathology, mild cognitive impairment (MCI) with moderate AD pathology, and AD. Among the AD-related miRNA expression changes, miR-107 was exceptional because miR-107 levels decreased significantly even in patients with …

Pioglitazone Inhibition Of Lipopolysaccharide-Induced Nitric Oxide Synthase Is Associated With Altered Activity Of P38 Map Kinase And Pi3k/Akt, Bin Xing, Tao Xin, Randy Lee Hunter, Guoying Bing

Neuroscience Faculty Publications

BACKGROUND: Previous studies have suggested that peroxisome proliferator activated receptor-gamma (PPAR-gamma)-mediated neuroprotection involves inhibition of microglial activation and decreased expression and activity of inducible nitric oxide synthase (iNOS); however, the underlying molecular mechanisms have not yet been well established. In the present study we explored: (1) the effect of the PPAR-gamma agonist pioglitazone on lipopolysaccharide (LPS)-induced iNOS activity and nitric oxide (NO) generation by microglia; (2) the differential role of p38 mitogen-activated protein kinase (p38 MAPK), c-Jun NH(2)-terminal kinase (JNK), and phosphoinositide 3-kinase (PI3K) on LPS-induced NO generation; and (3) the regulation of p38 MAPK, JNK, and PI3K by pioglitazone. …

Interspecific Hybridization Yields Strategy For South Pacific Filariasis Vector Elimination, Corey L. Brelsfoard, Yves Séchan, Stephen L. Dobson

Entomology Faculty Publications

BACKGROUND: Lymphatic filariasis (LF) is a leading cause of disability in South Pacific regions, where >96% of the 1.7 million population are at risk of LF infection. As part of current global campaign, mass drug administration (MDA) has effectively reduced lymphatic filiariasis prevalence, but mosquito vector biology can complicate the MDA strategy. In some regions, there is evidence that the goal of LF elimination cannot be attained via MDA alone. Obligate vector mosquitoes provide additional targets for breaking the LF transmission cycle, but existing methods are ineffective for controlling the primary vector throughout much of the South Pacific, Aedes polynesiensis. …

Making Forages Work Down On The Farm, Todd Clark

Forage Symposium at the Kentucky Cattlemen’s Convention

No abstract provided.

Making Forages Work Down On The Farm, Russell C. Hackley

Forage Symposium at the Kentucky Cattlemen’s Convention

No abstract provided.

Making Forages Work Down On The Farm, Jason Sandefur

Forage Symposium at the Kentucky Cattlemen’s Convention

No abstract provided.

Making Forages Work Down On The Farm, Lowell J. Clifford

Forage Symposium at the Kentucky Cattlemen’s Convention

No abstract provided.

Kentucky's Forage Resources, Garry D. Lacefield

Forage Symposium at the Kentucky Cattlemen’s Convention

Kentucky is a FORAGE state with approximately one-half of the total farmland occupied by forage grasses and legumes. We are very fortunate that we can grow many different species successfully. We have the natural resources and climate to grow most cool-season and warm-season species. We are fortunate that we usually have a relatively long growing season. We are usually blessed with rain, especially in spring (2007 was a major exception) with our annual rainfall approximately fifty inches. We are also fortunate that we have some of the absolute best forage-livestock producers in the World.

Foreword [2008], Garry D. Lacefield, Christi L. Forsythe

Forage Symposium at the Kentucky Cattlemen’s Convention

No abstract provided.

Genome-Wide Analysis Of The Interaction Between The Endosymbiotic Bacterium Wolbachia And Its Drosophila Host, Zhiyong Xi, Laurent Gavotte, Yan Xie, Stephen L. Dobson

Entomology Faculty Publications

BACKGROUND: Intracellular Wolbachia bacteria are obligate, maternally-inherited, endosymbionts found frequently in insects and other invertebrates. The success of Wolbachia can be attributed in part to an ability to alter host reproduction via mechanisms including cytoplasmic incompatibility (CI), parthenogenesis, feminization and male killing. Despite substantial scientific effort, the molecular mechanisms underlying the Wolbachia/host interaction are unknown.

RESULTS: Here, an in vitro Wolbachia infection was generated in the Drosophila S2 cell line, and transcription profiles of infected and uninfected cells were compared by microarray. Differentially-expressed patterns related to reproduction, immune response and heat stress response are observed, including multiple genes that have …

Alabama Summer: Suite For Orchestra With Structural And Harmonic Analysis, David Frank Mckee

University of Kentucky Doctoral Dissertations

An original composition in three movements for large orchestra. Following the score is a detailed analysis of the work, consisting of an introduction, three sections of analysis devoted to each of the three movements of the composition, and a short conclusion summarizing the analysis.

The Effect Of Pparγ Activation By Pioglitazone On The Lipopolysaccharide-Induced Pge2 And No Production: Potentialunderlying Alteration Of Signaling Transduction, Bin Xing

University of Kentucky Doctoral Dissertations

Microglia-mediated neuroinflammation plays an important role in the pathogenesis of Parkinson's disease (PD). Uncontrolled microglia activation produces major proinflammatory factors including cyclooxygenase 2 (COX-2) and inducible nitric oxide synthase (iNOS) that may cause dopaminergic neurodegeneration. Peroxisome proliferator-activated receptor γ (PPARγ) agonist pioglitazone has potent antiinflammatory property. We hypothesize pioglitazone protects dopaminergic neuron from lipopolysaccharide (LPS)-induced neurotoxicity by interacting with relevant signal pathways, inhibiting microglial activation and decreasing inflammatory mediators.

First, the neuroprotection of pioglitazone was explored. Second, the signaling transductions such as jun N-terminal kinase (JNK) and the interference with these pathways by pioglitazone were investigated. Third, the effect of …

The Mobility Of Fecal Indicator Microorganisms Within A Karst Groundwater Basin In The Inner Bluegrass Region, Kentucky, James Wade Ward

University of Kentucky Doctoral Dissertations

This project implemented novel approaches to assess the source, age, concentration and mobility of fecal indicator microorganisms within a karst groundwater system. Research was conducted in the well-characterized Blue Hole Spring karst groundwater basin in Versailles, Woodford County, Kentucky. At this site the AC/TC ratio and fecal coliform (FC) bacteria counts were used to delineate sources of fecal inputs and determine relative age of the fecal matter. An aging experiment using indicator bacteria (total coliform (TC) and atypical colonies (AC)), which approximated subsurface conditions, indicated that changes in the AC/TC ratio are likely to be retarded during bacterial transport through …

Map-Based Cloning Of An Anthracnose Resistance Gene In Medicago Truncatula, Shengming Yang

University of Kentucky Doctoral Dissertations

Anthracnose, caused by the fungal pathogen Colletotrichum trifolii, is one of the most destructive diseases of alfalfa worldwide. Cloning and characterization of the host resistance (R) genes against the pathogen will improve our knowledge of molecular mechanisms underlying host resistance and facilitate the development of resistant alfalfa cultivars. However, the intractable genetic system of cultivated alfalfa, owing to its tetrasomic inheritance and outcrossing nature, limits the ability to carry out genetic analysis in alfalfa. Nonetheless, the model legume Medicago truncatula, a close relative of alfalfa, provides a surrogate for cloning the counterparts of many agronomically important genes in …

Forage News [2008-01], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Heart of America Grazing Conference to be Held in Missouri
• Forage Producers to be Featured at KCA
• Plan to Attend the AFGC/SRM Forage Conference in Louisville January 26-31
• 2007 Variety Test Reports
• Clearing Up the Confusion Between Eating Meat, Cancer, and Obesity
• Teff Looks Good in Pennsylvania Trials
• Kentucky Facts
• Effect of Hay Feeding Methods on Cow Performance, Hay Waste, and Wintering Cost
• Kentucky's Agricultural Economy
• Upcoming Events

Packing Factors Of Feed Products In Storage Structures, Samuel G. Mcneill, Michael D. Montross, Sidney A. Thompson, I. Joseph Ross, Thomas C. Bridges

Biosystems and Agricultural Engineering Faculty Publications

Experiments were conducted to measure the changes in bulk density of cracked corn, corn meal, soybean meal, cotton seed meal, and distillers dried grain (without solubles) when subjected to simulated overburden pressures. All materials were tested at two moisture content levels (approximately 8% and 12% w.b.) and seven pressures between 0 and 69 kPa (0 and 10 psi). A mathematical model was fitted to the data to predict the bulk density of each feed ingredient as a function of pressure and moisture content. These relationships were inserted into a previously developed computer model to predict ingredient packing within conventional storage …

A History Of Art In Africa, Monica Blackmun Visonà, Robin Poynor, Herbert M. Cole

Art and Visual Studies Textbook Gallery

This is an adapted version of the second edition of A History of Art in Africa, published in 2008 by Pearson/Prentice Hall. It has been scanned and digitized with the original graphics and illustrations redacted for Monica Blackmun Visonà, who now holds copyright to the entire text. The text is complete, and chapters 1-5 include thumbnails with weblinks to alternate illustrations. Dr. Visonà is preparing a third edition of A History of Art in Africa for University of Michigan Publishing Services, which will appear both online and in print. As this textbook remains the most comprehensive art historical survey of …

State Regulation Of Complementary And Alternative Veterinary Therapies: Defining The Practice Of Veterinary Medicine In The 21st Century, Milton C. Toby

Kentucky Journal of Equine, Agriculture, & Natural Resources Law

No abstract provided.

The Pending Farmers' Market Fiasco: Small-Time Farmers, Part-Time Shoppers, And A Big-Time Problem, Brandon Baird

Kentucky Journal of Equine, Agriculture, & Natural Resources Law

No abstract provided.

Reining In The Horse Racing Industry: A Proposal For Federal Regulation Of Steroid Use In Racehorses, Jennifer M. Jabroski

Kentucky Journal of Equine, Agriculture, & Natural Resources Law

No abstract provided.

The Role Of Administrative Law In Regulating "Mad Cow Disease" As Explained In Creekstone Farms Premium Beef, Llc V. Department Of Agriculture, Courtney E. Ross

Kentucky Journal of Equine, Agriculture, & Natural Resources Law

No abstract provided.