Digital Commons Network^™

Kentucky Traffic Collision Facts 2008 Report, Kentucky Transportation Center, Kentucky State Police

Kentucky Transportation Center Research Report

KENTUCKY’S TRAFFIC COLLISION FACTS report for 2008 is based on collision reports submitted to the Kentucky State Police Records Branch. As required by Kentucky Revised Statutes 189.635, “every law enforcement agency whose officers investigate a vehicle accident of which a report must be made...shall file a report of the accident...within ten days after investigation of the accident upon forms supplied by the bureau.” The stated purpose of this requirement is to utilize data on traffic collisions for such purposes as will improve the traffic safety program in the Commonwealth. Data contained in this report are based solely on the observations …

Suicides Among Farmers In Three Southeastern States, 1990-1998, Steven R. Browning, Susan C. Westneat, Robert H. Mcknight

Epidemiology and Environmental Health Faculty Publications

Several studies have documented higher rates of suicide among farmers in comparison to other occupational groups, both in the U.S. and internationally. The purpose of this study is to describe the epidemiology of farmer suicides in three southeastern states (Kentucky, North Carolina, and South Carolina) during the nine-year period 1990-1998. Electronic death certificate data were obtained from the National Center for Health Statistics. Over the nine-year period, there were 590 deaths related to suicide (E-codes: 950-959) among farmers (occupation codes: 473, 474, 475, 477, and 479) in the states of Kentucky, North Carolina, and South Carolina. The results of this …

Assessment Of Water Quality Trends In The North Fork Of Kentucky River Basin: Focus On Pathogen Impairment, Kentucky Water Resources Research Institute

KWRRI Research Reports

This project consisted of analyzing existing water quality data for streams in the Upper Forks of Kentucky River Basin, with a particular focus on pathogen trends. According to the most recent 303(d) list of Kentucky waters, the majority of streams assessed in the Upper Forks of Kentucky River Basin are impaired for primary recreational contact as a result of pathogens. The state pathogen standard for primary contact recreation is expressed in a dual form which specifies that the 30-day geometric mean of fecal coliform counts not exceed 200 colonies per 100 mL (on a minimum of five samples) and not …

Summary Of Kentucky River Watershed Watch 2008 Water Samplig Results, Kentucky Water Resources Research Institute, Kentucky River Authority

Kentucky River Watershed Watch

No abstract provided.

“There's Danger Here, Cherie!”: Liability For The Promotion And Marketing Of Drugs And Medical Devices For Off-Label Uses, Richard C. Ausness

Law Faculty Scholarly Articles

Physicians often prescribe prescription drugs and other medications for uses that are not approved by the Food and Drug Administration ("FDA"), and such "off label" prescription is widely accepted within the medical community as a legitimate form of treatment. However, the federal government discourages off-label prescription and use in various ways. For example, the FDA restricts the dissemination of information by drug companies about potential off-label therapies. In addition, federally funded health insurance programs such as Medicaid do not reimburse health care providers for off-label uses. Because drug companies make large profits from off-label prescriptions, they are often tempted to …

Groundwater Quality In Watersheds Of The Big Sandy River, Little Sandy River, And Tygarts Creek (Kentucky Basin Management Unit 5), R. Stephen Fisher, Bart Davidson, Peter T. Goodmann

Report of Investigations--KGS

The Kentucky Geological Survey and the Kentucky Division of Water are evaluating groundwater quality throughout the commonwealth to determine regional conditions, assess impacts of nonpoint-source contaminants, provide a baseline for tracking changes, and provide essential information for environmental-protection and resource-management decisions. These evaluations include summarizing existing regional groundwater-quality data and reporting the results of expanded, focused groundwater collection programs in specific areas. This report summarizes groundwater sampling and analysis in Kentucky basin management unit 5 (watersheds of the Big Sandy River, Little Sandy River, and Tygarts Creek in eastern Kentucky).

Thirty wells and springs were sampled quarterly between the fall …

Assessment Of Synaptic Function During Short-Term Facilitation In Motor Nerve Terminals In The Crayfish, Mohati Desai-Shah, Kert Viele, G. Sparks, J. Nadolski, B. Hayden, V. K. Srinivasan, Robin L. Cooper

Biology Faculty Publications

An enhanced buildup of [Ca²⁺]i occurs during short-term facilitation (STF) at the crayfish neuromuscular junction (NMJ). As a model system, this NMJ allows discrete postsynaptic quantal events to be counted and characterized in relation to STF. Providing 10 pulses, at 20 and 40Hz, we monitored postsynaptic quantal events over a discrete region of a nerve terminal with a focal macropatch electrode. Characteristics of quantal events were clustered into groups by peak amplitude and time to the peak amplitude. Since the synapses at this NMJ have varied spacing of active zones, number of active zones and synaptic size, the …

Presynaptic Depression In Phasic Motor Nerve Terminals And Influence Of 5-Ht On Vesicle Dynamics, A. F. M. Johnstone, S. S. Kellie, Robin L. Cooper

Biology Faculty Publications

Synaptic depression that is induced by electrical stimulation of the glutamatergic neuromuscular junction (NMJ) of the crayfish can be offset by recruitment of vesicles from a presynaptic reserve pool. This recruitment occurs following treatment of the NMJ with serotonin (5-HT), which results in a delay in the onset of synaptic depression induced by high frequency stimulation. The results of this study demonstrate that the releasable vesicles are insufficiently replenished during high frequency stimulation and that the readily releasable pool of vesicles (RRP) can be enhanced by the reserve pool (RP) in the presence of 5-HT. Anatomical visualization of vesicular pools …

Color Multiplexed Single Pattern Sli, Neelima Mandava

University of Kentucky Master's Theses

Structured light pattern projection techniques are well known methods of accurately capturing 3-Dimensional information of the target surface. Traditional structured light methods require several different patterns to recover the depth, without ambiguity or albedo sensitivity, and are corrupted by object movement during the projection/capture process. This thesis work presents and discusses a color multiplexed structured light technique for recovering object shape from a single image thus being insensitive to object motion.

This method uses single pattern whose RGB channels are each encoded with a unique subpattern. The pattern is projected on to the target and the reflected image is captured …

Recent Advances In Medicago Truncatula Genomics, Jean-Michel Ané, Hongyan Zhu, Julia Frugoli

Plant and Soil Sciences Faculty Publications

Legume rotation has allowed a consistent increase in crop yield and consequently in human population since the antiquity. Legumes will also be instrumental in our ability to maintain the sustainability of our agriculture while facing the challenges of increasing food and biofuel demand. Medicago truncatula and Lotus japonicus have emerged during the last decade as two major model systems for legume biology. Initially developed to dissect plant-microbe symbiotic interactions and especially legume nodulation, these two models are now widely used in a variety of biological fields from plant physiology and development to population genetics and structural genomics. This review highlights …

Thou Shall Not Hunt: A Historical Introduction To And Discussion Of The Modern Debate Over Sunday Hunting Laws, Mike Balestra

Kentucky Law Journal

No abstract provided.

Let Us Eat Lettuce (And Spinach): Is The Bioterrorism Preparedness Act Enough Or Is An Independent Food Safety Agency Required?, Robert M. Yoke

Kentucky Law Journal

No abstract provided.

Organizational Learning In A High-Risk Environment: Responding To An Anthrax Outbreak, Shari R. Veil, Timothy L. Sellnow

Communication Faculty Publications

The National Center for Food Protection and Defense, a Department of Homeland Security-sponsored Center of Excellence, identified and endorses 10 best practices for risk and crisis communication. The best practices model is designed to aid organizations of all types in preparing for and learning from crisis events. This analysis applied the best practices model to a case study of an anthrax outbreak that fostered a full-blown crisis response. This study analyzes the learning experience of the crisis cohort group that responded to the crisis. The researchers contend that by evaluating a crisis situation using best practices as a benchmarking procedure, …

Micrornas (Mirnas) In Neurodegenerative Diseases, Peter T. Nelson, Wang-Xia Wang, Bernard W. Rajeev

Sanders-Brown Center on Aging Faculty Publications

Aging-related neurodegenerative diseases (NDs) are the culmination of many different genetic and environmental influences. Prior studies have shown that RNAs are pathologically altered during the inexorable course of some NDs. Recent evidence suggests that microRNAs (miRNAs) may be a contributing factor in neurodegeneration. miRNAs are brain-enriched, small (~22 nucleotides) non-coding RNAs that participate in mRNA translational regulation. Although discovered in the framework of worm development, miRNAs are now appreciated to play a dynamic role in many mammalian brain-related biochemical pathways, including neuroplasticity and stress responses. Research about miRNAs in the context of neurodegeneration is accumulating rapidly, and the goal of …

Molecular, Genetic And Biochemical Characterization Of Oleic Acid- And Glycerol-Mediated Signaling In Plant Defense, Srivathsa C. Venugopal

Theses and Dissertations--Plant Pathology

Oleic acid (18:1) is one of the important monounsaturated fatty acids, which is synthesized upon desaturation of stearic acid and this reaction is catalyzed by the SSI2 encoded stearoyl-acyl-carrier-protein-desaturase. A mutation in SSI2 leads to constitutive activation of salicylic acid (SA)-mediated defense responses. Consequently, these plants accumulate high levels of SA and show enhanced resistance to bacterial and oomycete pathogens. Replenishing 18:1 levels in ssi2 plants, via a second site mutation in GLY1 encoded glycerol-3-phosphate (G3P) dehydrogenase, suppresses all the ssi2-triggered phenotypes. Study of mechanism(s) underlying gly1-mediated suppression of ssi2 phenotypes showed that 18:1 levels are regulated via …

Sr-Bi Protects Against Endotoxemia In Mice Through Its Roles In Glucocorticoid Production And Hepatic Clearance, Lei Cai, Ailing Ji, Frederick C. De Beer, Lisa R. Tannock, Deneys R. Van Der Westhuyzen

Internal Medicine Faculty Publications

Septic shock results from an uncontrolled inflammatory response, mediated primarily by LPS. Cholesterol transport plays an important role in the host response to LPS, as LPS is neutralized by lipoproteins and adrenal cholesterol uptake is required for antiinflammatory glucocorticoid synthesis. In this study, we show that scavenger receptor B-I (SR-BI), an HDL receptor that mediates HDL cholesterol ester uptake into cells, is required for the normal antiinflammatory response to LPS-induced endotoxic shock. Despite elevated plasma HDL levels, SR-BI–null mice displayed an uncontrollable inflammatory cytokine response and a markedly higher lethality rate than control mice in response to LPS. In addition, …

Using Microhistological Techniques To Predict Botanical Composition Of Horse Diets On Cool-Season Grass Pasture, Jesse Ira Morrison

University of Kentucky Master's Theses

Microhistological analysis is a highly effective microscopic technique of determining botanical composition of animal diets by visual recognition of fecal plant fragments, and has been widely used in range studies. The objective of this study was to use microhistological techniques to predict the botanical composition of domesticated horse diets when grazing mixed cool-season pasture. Samples of tall fescue [Lolium arundinaceum (Schreb.) S.J. Darbyshire], Kentucky bluegrass (Poa pratensis L.) and orchardgrass (Dactylis glomerata L.) were evaluated for microscopically unique characteristics. Cool season pasture grazing studies were conducted in October 2006 and May 2007 in Lexington, KY. Eight thoroughbred mares were placed …

Reciprocal Regulation Of Par-4 And Caspase-8 In The Trail Signaling Pathway, Padhma Ranganathan

University of Kentucky Doctoral Dissertations

Par‐4 is a pro‐apoptotic tumor suppressor that is mutated, suppressed or inactivated in cancer. Par‐4 exploits components of the extrinsic pathway to cause apoptosis selectively of cancer cells. This study identified Par‐4 as an essential component of the apoptotic pathway induced by TRAIL, which selectively targets cancer cells. RNA interference‐mediated knockdown of Par‐4 rendered cancer cells unresponsive to TRAIL‐induced apoptosis. Cells with knocked‐down levels of Par‐4 were deficient in the activation of the apoptosis‐initiator caspase‐8 and the apoptosis‐effector caspase‐3 in response to TRAIL. Par‐4 was identified as a critical mediator of membrane translocation of caspase‐8 and the adapter protein FADD. …

Effect Of The SmartstepTm Stabilization System On Balance In Older Adults In An Independent Living Residence, Ann L. Livengood

University of Kentucky Doctoral Dissertations

An increase in postural sway is one of the risk factors that have been linked to an increased incidence of falls in the older adult population. Researchers have shown that peripheral sensation is crucial in maintaining a static posture for adults of all ages. It has been reported that older adults have decreased tactile sensation of the plantar surface of their feet. and when the sensory feedback was increased older adults had improved postural control. It was hypothesized that facilitation of the sole of the foot with the use of a semirigid foot orthotic would result in improved postural stability …

The Influence Of Veterinary Schools On The Veterinary Labor Market, Kyle Bosh

MPA/MPP/MPFM Capstone Projects

Problem Statement:

Does the presence of a veterinary school in a state influence the supply of veterinarians practicing in that state? Citizens and animals in states without a veterinary school are at a disadvantage if the presence of a veterinary school significantly improves public health and access to medical services in those states that have a veterinary school.

Research Strategy:

The unit of analysis in this study is 50 states and the District of Columbia. The study is designed as a cross-sectional analysis of 2006 pet, 2002 livestock, 2006 human, labor, and economic data. The study uses simultaneous equations to …

Role Of The Reactive Oxygen Species Peroxynitrite In Traumatic Brain Injury, Ying Deng

University of Kentucky Doctoral Dissertations

Reactive oxygen species (ROS) is cytotoxic to the cell and is known to contribute to secondary cell death following primary traumatic brain injury (TBI). We described in our study that PN is the main mediator for both lipid peroxidation and protein nitration, and occurred almost immediately after injury. As a downstream factor to oxidative damage, the peak of Ca2+-dependent, calpainmediated cytoskeletal proteolysis preceded that of neurodegeneration, suggesting that calpain-mediated proteolysis is the common pathway leading to neuronal cell death. The time course study clearly elucidated the interrelationship of these cellular changes following TBI, provided window of opportunity for pharmacological intervention. …

Determination Of The Extravascular Burden Of Carbon Monoxide (Co) On Human Heart, Kinnera Erupaka

University of Kentucky Master's Theses

Noninvasive measurements of myocardial carboxymyoglobin levels (%MbCO) and oxygen tensions (PtO2) are difficult to obtain experimentally. We have developed a compartmental model which allows prediction of myocardial %MbCO levels and PtO2 for varied carbon monoxide (CO) exposures. The cardiac compartment in the model consists of vascular subcompartments which contain two tissue subcompartments varying in capillary density. Mass-balance equations for oxygen (O2) and CO are applied for all compartments. Myocardial oxygen consumption and blood flow are quantified from predictive formulas based on heart rate. Model predictions are validated with experimental data at normoxia, hypoxia, exercise and hyperoxia. CO exposures of varying …

Challenges And Opportunities To Rural Nutrition Education Programs In Kentucky's Superfund Communities, Carolyn L. Hofe

University of Kentucky Master's Theses

The National Electric Coil Company/Cooper Industries, Inc. plant in Harlan County, Kentucky was a mining support operation primarily engaged in the cleaning and repair of mining equipment from 1951 to 1987. Trichloroethylene (TCE) and degradation byproducts, polychlorinated biphenyls (PCBs), and vinyl chloride were released into the areas surrounding the plant periodically for decades. Routine water sampling of area wells by the Kentucky Department of Environmental Protection Division of Water revealed significant levels of TCE, PCBs, and vinyl chloride. The toxicology of these chemicals implicates various systems, including cardiovascular, dermal, endocrine, and neurological.

University of Kentucky’s Superfund Basic Research Program’s (UK-SBRP) …

Content Preparation Of Pre-Service Agricultural Education Teachers And Its Influence On Their Content Knowledge, Amber Houck

University of Kentucky Master's Theses

Content knowledge preparation for teachers is a crucial component of the modal curriculum model for education. The purpose of this study was to determine if the amount and quality of coursework preparation of pre-service University of Kentucky agriculture teachers influences their content knowledge as defined by the Praxis II agriculture exam scores. This study concluded that there was variability in coursework preparation of pre-service agriculture teachers at the University of Kentucky. Praxis II exam scores of pre-service teachers indicated that most students are meeting an adequate content knowledge level based on the exam material. It can also be concluded that …

An Analysis Of Kentucky Equestrian Trail Riders: Determining Rider Behaviors And Valuing Site Amenities That Contribute To Repeat Visits, Katharine Auchter

University of Kentucky Master's Theses

The purpose of this travel cost study is to determine how rider behaviors and site characteristics influence repeat visits for equestrian trail riding in Kentucky. Primary data was collected via a survey developed and administered to trail riders in person and online. The average surveyed trail rider tends to be female, about 46 years old, with some higher education, and an annual household income of $65,000. She makes 11 trips to a specified site per year, 8 of which are daytrips, usually in the fall, and traveling 132 miles round trip. From other information gathered, an index of trail characteristics …

Microbial Community Structure Dynamics In Ohio River Sediments During Reductive Dechlorination Of Pcbs, Andres Enrique Nunez

University of Kentucky Doctoral Dissertations

The entire stretch of the Ohio River is under fish consumption advisories due to contamination with polychlorinated biphenyls (PCBs). In this study, natural attenuation and biostimulation of PCBs and microbial communities responsible for PCB transformations were investigated in Ohio River sediments.

Natural attenuation of PCBs was negligible in sediments, which was likely attributed to low temperature conditions during most of the year, as well as low amounts of available nitrogen, phosphorus, and organic carbon. Moreover, surface sediments were relatively oxidized, as indicated by the prevalence of aerobic bacteria such as beta- Proteobacteria, alpha-Proteobacteria, Sphingobacteria, and Nitrospira in 16S rRNA sediment …

Parametrization And Shape Reconstruction Techniques For Doo-Sabin Subdivision Surfaces, Jiaxi Wang

University of Kentucky Master's Theses

This thesis presents a new technique for the reconstruction of a smooth surface from a set of 3D data points. The reconstructed surface is represented by an everywhere -continuous subdivision surface which interpolates all the given data points. And the topological structure of the reconstructed surface is exactly the same as that of the data points. The new technique consists of two major steps. First, use an efficient surface reconstruction method to produce a polyhedral approximation to the given data points. Second, construct a Doo-Sabin subdivision surface that smoothly passes through all the data points in the given data set. …

Hypothalamic Mediation Of Acute Increases In Arterial Blood Pressure And Renal Sympathetic Nerve Activity During Electrical Stimulation Of The Lamina Temrinalis, Samuel Paterson Carmichael Ii

University of Kentucky Master's Theses

Discrete electrical stimulation of the organum vasculosum of the lamina terminalis (OVLT) produces sympathetically-mediated increases in peripheral resistance and arterial blood pressure (ABP). Since efferent fibers from the lamina terminalis innervate the kidney through polysynaptic connections, the present study determined whether electrical stimulation of the OVLT increased sympathetic outflow to the kidney. In anesthetized male, Sprague-Dawley rats (n=5) electrical stimulation of OVLT neurons produced frequency and current intensity dependent increases in renal sympathetic nerve activity (RSNA) and ABP that were abolished by ganglionic blockade with the nicotinic antagonist chlorisondamine (5mg/kg,i.v.). Since neurons from the OVLT terminate within the hypothalamic paraventricular …

Removal Of Manganese From An Alkaline Mine Drainage Using A Bioreactor With Different Organic Carbon Sources, Jared D. Edwards

University of Kentucky Master's Theses

The treatment of Mn and SO₄^2- contaminated mine drainage via a sulfate reducing bioreactor is expected to result in near-permanent immobilization of significant amounts of Mn and a portion of the sulfates within the matrix. This study tested several different combinations of organic amendments and inorganic substrates in an attempt to optimize sulfate reducing conditions and Mn removal capacity. Five different organic carbon sources, including corn mash, wood mulch, biosolids, soybean oil, and sorghum syrup in combination with five different inorganic substrates, including creek sediment, marble and limestone chips, polished gravel, and sand were tested in batch experiments. …

Cloning, Characterization And Subcellular Localization Of The N (Nucleocapsid) And P (Phosphoprotein) Protein Of The Sydv (Potato Yellow Dwarf Virus Sanguinolenta Strain), Debasish Ghosh

University of Kentucky Doctoral Dissertations

Potato yellow dwarf virus (PYDV) is the type member of the genus Nucleorhabdovirus. The virus replicates in the nuclei of infected cells and mature virions accumulate in the perinuclear space after viral cores bud through the inner nuclear membrane. The virus was first described as an extremely destructive pathogen of potato (Solanum tuberosum) and other members of family Solanaceae. There are two different strains of PYDV based on their insect-vector specificity, namely SYDV (sanguinolenta strain) and CYDV (constricta strain). PYDV is considered a model system to study virus-vector relationship, particularly for agriculturally harmful rhabdoviruses. However, very little is known about …