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A Model To Integrate Sustainability Into The User-Centered Design Process, Adrienne Shevonne Brown Jan 2011

A Model To Integrate Sustainability Into The User-Centered Design Process, Adrienne Shevonne Brown

Electronic Theses and Dissertations

With concerns for the environment becoming more prevalent in business and the government, it is increasingly important to re-evaluate and update processes to include sustainability considerations early in the design process. In response to this charge, this research effort was designed to integrate sustainability factors into the usercentered design process. The results of this research highlight the benefits of sustainability requirement planning, as well as those derived from integrating sustainability into the current user-centered design model

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Spectrum Sharing And Service Pricing In Dynamic Spectrum Access Networks, Swastik Kumar Brahma Jan 2011

Spectrum Sharing And Service Pricing In Dynamic Spectrum Access Networks, Swastik Kumar Brahma

Electronic Theses and Dissertations

Traditionally, radio spectrum has been statically allocated to wireless service providers (WSPs). Regulators, like FCC, give wireless service providers exclusive long term licenses for using specific range of frequencies in particular geographic areas. Moreover, restrictions are imposed on the technologies to be used and the services to be provided. The lack of flexibility in static spectrum allocation constrains the ability to make use of new technologies and the ability to redeploy the spectrum to higher valued uses, thereby resulting in inefficient spectrum utilization [23, 38, 42, 62, 67]. These limitations have motivated a paradigm shift from static spectrum allocation towards …

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Landfill Gas To Energy Incentives And Benefits, Hamid R. Amini Jan 2011

Landfill Gas To Energy Incentives And Benefits, Hamid R. Amini

Electronic Theses and Dissertations

Municipal solid waste (MSW) management strategies typically include a combination of three approaches, recycling, combustion, and landfill disposal. In the US approximately 54% of the generated MSW was landfilled in 2008, mainly because of its simplicity and cost-effectiveness. However, landfills remain a major concern due to potential landfill gas (LFG) emissions, generated from the chemical and biological processes occurring in the disposed waste. The main components of LFG are methane (50-60%) and carbon dioxide (40-50%). Although LFG poses a threat to the environment, if managed properly it is a valuable energy resource due to the methane content. Currently there are …

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Stress Shielding Minimized In Femoral Hip Implants A Finite Element Model Optimized By Virtual Compatibility, Christian E. Feldt Jan 2011

Stress Shielding Minimized In Femoral Hip Implants A Finite Element Model Optimized By Virtual Compatibility, Christian E. Feldt

Electronic Theses and Dissertations

Bone mechanics and traditional implant materials produce a recurring problem for patients of total hip arthroplasty (THA): the bone is “shielded” from the loading it has become accustomed to over many years of development. Bone adheres to what is called “Wolff’s Law”, meaning it is an adaptive structure which adjusts its geometry based on the loads experienced over its life (Pearson; Goldstein). As the new femoral hip implant transmits reduced stresses to the remaining bone, bone tissue atrophies at the interface, permitting loosening of the implant, pain, and thereby obliging additional surgery to correct the issue (Meade). In the present …

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Mass Conservation Analysis For The Lower St. Johns River Using Continuous And Discontinuous Galerkin Finite Element Methods, Lillie E. Thomas Jan 2011

Mass Conservation Analysis For The Lower St. Johns River Using Continuous And Discontinuous Galerkin Finite Element Methods, Lillie E. Thomas

Electronic Theses and Dissertations

This thesis provides a mass conservation analysis of the Lower St. Johns River for the purpose of providing basis for future salinity transport modeling. The analysis provides an assessment of the continuous (CG) and discontinuous (DG) Galerkin finite element methods with respect to their mass conservation properties. The following thesis also presents a rigorous literature review pertaining to salinity transport in the Lower St. Johns River, from which this effort generates the data used to initialize and validate numerical simulations. Two research questions are posed and studied in this thesis: can a DG-based modeling approach produce mass conservative numerical solutions; …

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Nutrient Removal From Urban Stormwater Using Floating Treatment Wetland System, Md Kamrul Islam Jan 2011

Nutrient Removal From Urban Stormwater Using Floating Treatment Wetland System, Md Kamrul Islam

Electronic Theses and Dissertations

Despite the technology advancement, degradation of water quality due to stormwater continues to be a significant threat to the water and ecosystems due to the exponential growth of industries and agricultural enterprises that discharge stormwater. These anthropogenic activities are the sources of high nitrogen and phosphorus quantities in stormwater, which is responsible for eutrophication phenomena and deterioration of public health. Floating Treatment Wetlands (FTWs) are a potential solution to this problem. Both microcosm and mesocosm level studies were conducted for the effective removal of nutrients in stormwater wet detention ponds with different sorption media under varying nutrient concentrations and weather …

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Intelligent Design, Jillian Amistoso Dudziak Jan 2011

Intelligent Design, Jillian Amistoso Dudziak

Electronic Theses and Dissertations

As human beings we are designed and created in a fabric that is profound and complex. We are built with a framework where mind and body work in a concerted effort to maintain our lives automatically. A deep and defining part of our existence as humans is not just the innate desire to live but to live in consistent well-being--emotionally, physically, and mentally. I believe when we incorporate our knowledge of human physiology into our creative process then we allow ourselves a greater opportunity to create an authentic connection with our intended audience. My work during the past three years …

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Bayesian Model Selection For Classification With Possibly Large Number Of Groups, Justin Kyle Davis Jan 2011

Bayesian Model Selection For Classification With Possibly Large Number Of Groups, Justin Kyle Davis

Electronic Theses and Dissertations

The purpose of the present dissertation is to study model selection techniques which are specifically designed for classification of high-dimensional data with a large number of classes. To the best of our knowledge, this problem has never been studied in depth previously. We assume that the number of components p is much larger than the number of samples n, and that only few of those p components are useful for subsequent classification. In what follows, we introduce two Bayesian models which use two different approaches to the problem: one which discards components which have “almost constant” values (Model 1) and …

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Self-Assembly Of Amyloid Aggregates Simulated With Molecular Dynamics, Workalemahu Mikre Berhanu Jan 2011

Self-Assembly Of Amyloid Aggregates Simulated With Molecular Dynamics, Workalemahu Mikre Berhanu

Electronic Theses and Dissertations

Amyloids are highly ordered cross-β sheet aggregates that are associated with many diseases such as Alzheimer‟s, type II diabetes and prion diseases. Recently a progress has been made in structure elucidation, environmental effects and thermodynamic properties of amyloid aggregates. However, detailed understanding of how mutation, packing polymorphism and small organic molecules influence amyloid structure and dynamics is still lacking. Atomistic modeling of these phenomena with molecular dynamics (MD) simulations holds a great promise to bridge this gap. This Thesis describes the results of MD simulations, which provide insight into the effects of mutation, packing polymorphism and molecular inhibitors on amyloid …

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Sage Collection, Ucf Libraries Jan 2011

Sage Collection, Ucf Libraries

Technology Fee Funded Projects

The UCF Libraries is requesting $124,450 to purchase the 2011 Sage Deep Backfile Collection. Sage Publications, Inc. is a respected academic publisher with strong content in business, humanities, social sciences, science, technology, and medicine. Sage journals, books, and electronic media are used by scholars, practitioners, researchers, and students. The UCF Libraries continues to pursue complete access to content from major academic publishers to meet current needs and to ensure long term access to the content. The Sage Deep Backfile Collection, with its strong humanities content, will complement the Complete Springer Archives, purchased with 2010 Technology Fee funding, which has a …

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Analytic & Numerical Study Of A Vortex Motion Equation, Daniel Bueller Jan 2011

Analytic & Numerical Study Of A Vortex Motion Equation, Daniel Bueller

Electronic Theses and Dissertations

A nonlinear second order differential equation related to vortex motion is derived. This equation is analyzed using various numerical and analytical techniques including finding approximate solutions using a perturbative approach.

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Algebraic Aspects Of (Bio) Nano-Chemical Reaction Networks And Bifurcations In Various Dynamical Systems, Teng Chen Jan 2011

Algebraic Aspects Of (Bio) Nano-Chemical Reaction Networks And Bifurcations In Various Dynamical Systems, Teng Chen

Electronic Theses and Dissertations

The dynamics of (bio) chemical reaction networks have been studied by different methods. Among these methods, the chemical reaction network theory has been proven to successfully predicate important qualitative properties, such as the existence of the steady state and the asymptotic behavior of the steady state. However, a constructive approach to the steady state locus has not been presented. In this thesis, with the help of toric geometry, we propose a generic strategy towards this question. This theory is applied to (bio)nano particle configurations. We also investigate Hopf bifurcation surfaces of various dynamical systems.

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Development Of Molecular And Cellular Imaging Tools To Evaluate Gene And Cell Based Therapeutic Strategies In Vivo, Jixiang Xia Jan 2011

Development Of Molecular And Cellular Imaging Tools To Evaluate Gene And Cell Based Therapeutic Strategies In Vivo, Jixiang Xia

Electronic Theses and Dissertations

Molecular imaging modalities are important tools to evaluate the efficacy of gene delivery systems and cell-based therapies. Development and application of these modalities will advance our understanding of the mechanism of transgene expression and cell fate and functions. Physical gene transfer methods hold many advantages over viral vectors among gene therapeutic strategies. Here, we evaluated the efficacy of biolistic (“gene gun”) gene targeting to tissues with non-invasive bioluminescence imaging (BLI) methods. Plasmids carrying the firefly luciferase reporter gene were transfected into mouse skin and liver using biolistics, and BLI was measured at various time points after transfer. With optimized DNA …

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Homologous Pairing Through Dna Driven Harmonics-- A Simulation Investigation, Richard J. Calloway Jan 2011

Homologous Pairing Through Dna Driven Harmonics-- A Simulation Investigation, Richard J. Calloway

Electronic Theses and Dissertations

The objective of this research is to determine if a better understanding of the “molecule of life”, deoxyribonucleic acid or DNA can be obtained through Molecular Dynamics (MD) modeling and simulation (M&S) using contemporary MD M&S. It is difficult to overstate the significance of the DNA molecule. The now-completed Human Genome Project stands out as the most significant testimony yet to the importance of understanding DNA. The Human Genome Project (HGP) enumerated many areas of application of genomic research including molecular medicine, energy sources, environmental applications, agriculture and livestock breeding to name just a few. (Science, 2008) In addition to …

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Transitions How Individuals In The Transgender Community Use Impression Management, Candice S. Campanaro Jan 2011

Transitions How Individuals In The Transgender Community Use Impression Management, Candice S. Campanaro

Electronic Theses and Dissertations

There exists an extensive body of literature focusing on impression management, with numerous studies addressing the effects of gender performance on members of the GLBT community. Despite a growing body of literature that centers on the differences between the genders, there exists a lack of research regarding the transgender community‟s ability to use impression management. This exploratory study addresses subjective experiences regarding transgender identity, and concentrates on self-definition and impression management in everyday life. It was found through interviews that “transition” is a temporary state that relies heavily on “passing” through impression management to create a new gender status. Once …

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Florida Superintendents' Views Related To The Involuntary Removal Of School Principals, Christopher S. Bernier Jan 2011

Florida Superintendents' Views Related To The Involuntary Removal Of School Principals, Christopher S. Bernier

Electronic Theses and Dissertations

This study sought to determine how external influences designed to improve student achievement and school performance such as No Child Left Behind Act of 2001, The Florida A+ Program, and the Differentiated Accountability model impacted Florida school superintendents’ prioritization of the Florida Principal Leadership Standards. In the study, major questions addressed concerned (a) the demographic characteristics of the school principals and superintendents involved in the removal of a school principal within the state of Florida; (b) the relationship between the problems a principal encountered and the Florida Principal Leadership Standards; (c) the Florida Principal Leadership Standards that were most often …

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Framing Racial Inequality Reassessing The Effect Of Religion On Racial Attitudes, Jerrold C. Kaufman Ii Jan 2011

Framing Racial Inequality Reassessing The Effect Of Religion On Racial Attitudes, Jerrold C. Kaufman Ii

Electronic Theses and Dissertations

Building on previous work on racial attitudes among the religious, this study reassesses the effects of religion on individuals’ beliefs about racial inequality. This study relies on recent developments in the sociology of culture, which conceives of culture as a frame through which individuals interpret the world in which they inhabit (Benford and Snow 2000; Harding 2007; Small 2002, 2004). Religion is held to be an important social institution that provides substance to the frames that individuals employ for interpreting racial inequality. Two particular developments from this literature inform this study: first, that individuals can employ different, even contradictory, frames …

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Pretend Land, Detrachia M. Neely Jan 2011

Pretend Land, Detrachia M. Neely

HIM 1990-2015

The bond between a mother and child is thought to be sacred. It is a phenomenon that society seems to expect as axiomatic based on the single biological fact that a woman carries her child, creating an inseparable bond; even for non-biological mothers, the bond is perceived as one of supreme importance. What happens to the mother and to the child, if this sacred bond is broken? The intent of this thesis was to focus on the perceived bond between mother and child and turn it on its head. As a work of fiction, Pretend Land is a series of …

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The Effects Of Attention Deficit/Hyperactivity Disorder On Fixations And Saccades During A Simulated Driving Task, Jessica R. Michaelis Jan 2011

The Effects Of Attention Deficit/Hyperactivity Disorder On Fixations And Saccades During A Simulated Driving Task, Jessica R. Michaelis

HIM 1990-2015

Individuals who have Attention Deficit/Hyperactivity Disorder (ADHD) experience adverse effects relating to driving; in addition, they experience deficits in scanning ability (Barkely et.al, 1996; Fischer et al., 2007; Munoz et al., 2003; Naja-Raja et al., 2007). The present study examined the effects of ADHD on eye tracking while driving. Ten participants consisting of both ADHD and individuals who do not have ADHD were included in this study. It was hypothesized that individuals who have ADHD will make more saccadic eye movements and thus shorter fixations than individuals who do not have ADHD. Furthermore, it was hypothesized that despite the fact …

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Art In The Light Of Knowing A Cognitive Approach To The Creative Process, Victor M. Knoe Jan 2011

Art In The Light Of Knowing A Cognitive Approach To The Creative Process, Victor M. Knoe

HIM 1990-2015

Art can be an elusive concept. Neither an outsider nor a professional artist is immune to abstractions in the attempt to describe it. Every individual must necessarily come from their own, unique perspective. The obstacles that we experience in defining the essence of art can be better understood if we see them as a gauge for our historical period. Given the limits of our contemporary conditions, it seems impossible that we may ever overcome the vast chasms that imprison us. We are discouraged to build bridges, at almost every turn, by the suggestive hopelessness of the abysmal distances between us. …

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Direct Patterning Of Coplanar Polyethylene Glycol Alkylsilane Monolayers By Deep-Ultraviolet Photolithography As A General Method For High Fidelity, Long-Term Cell Patterning And Culture, Kerry Wilson, Maria Stancescu, Mainak Das, John Rumsey, James Hickman Jan 2011

Direct Patterning Of Coplanar Polyethylene Glycol Alkylsilane Monolayers By Deep-Ultraviolet Photolithography As A General Method For High Fidelity, Long-Term Cell Patterning And Culture, Kerry Wilson, Maria Stancescu, Mainak Das, John Rumsey, James Hickman

Faculty Bibliography 2010s

This manuscript details a general method for patterning coplanar alkylsilane monolayers using deep-ultraviolet photolithography that has broad application for high fidelity patterning of cells of varying phenotype in long-term cultures. A polyethylene glycol monolayer was formed on a silica substrate and then patterned using 193 nm light from an ArF excimer laser. The regions of photoablation were then rederivatized with (3-trimethoxysilyl propyl) diethyltriamine (DETA), yielding high contrast cytophilic islands that promoted cell adhesion and growth. Rat hippocampal neurons, motoneurons, and myoblasts were then cultured in a defined, serum-free medium on the patterned surfaces for periods in excess of 40 days. …

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The Accuracy Of Climate Models' Simulated Season Lengths And The Effectiveness Of Grid Scale Correction Factors, Wade E. Winterhalter Jan 2011

The Accuracy Of Climate Models' Simulated Season Lengths And The Effectiveness Of Grid Scale Correction Factors, Wade E. Winterhalter

Faculty Bibliography 2010s

Global climate change is expected to impact biological populations through a variety of mechanisms including increases in the length of their growing season. Climate models are useful tools for predicting how season length might change in the future. However, the accuracy of these models tends to be rather low at regional geographic scales. Here, I determined the ability of several atmosphere and ocean general circulating models (AOGCMs) to accurately simulate historical season lengths for a temperate ectotherm across the continental United States. I also evaluated the effectiveness of regional-scale correction factors to improve the accuracy of these models. I found …

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Crmp5 (Collapsin Response Mediator Protein 5) Regulates Dendritic Development And Synaptic Plasticity In The Cerebellar Purkinje Cells, Naoya Yamashita, Bedrich Mosinger, Arpita Roy, Mari Miyazaki, Kozue Ugajin, Fumio Nakamura, Yukio Sasaki, Kazuhiko Yamaguchi, Pappachan Kolattukudy, Yoshio Goshima Jan 2011

Crmp5 (Collapsin Response Mediator Protein 5) Regulates Dendritic Development And Synaptic Plasticity In The Cerebellar Purkinje Cells, Naoya Yamashita, Bedrich Mosinger, Arpita Roy, Mari Miyazaki, Kozue Ugajin, Fumio Nakamura, Yukio Sasaki, Kazuhiko Yamaguchi, Pappachan Kolattukudy, Yoshio Goshima

Faculty Bibliography 2010s

Collapsin response mediator protein 5 (CRMP5) is one of the CRMP members that expresses abundantly in the developing brain. To examine the in vivo function of CRMP5, we generated crmp5-deficient (crmp5(-/-)) mice. Anti-calbindin immunofluorescence studies of crmp5(-/-) mice revealed aberrant dendrite morphology; specifically, a decrease in the size of soma and diameter of primary dendrite of the cerebellar Purkinje cells at postnatal day 21 (P21) and P28, but not at P14. Coincidentally, CRMP5 is detected in Purkinje cells at P21 and P28 from crmp5(+/-) mice. In cerebellar slices of crmp5(-/-) mice, the induction of long-term depression of excitatory synaptic transmission …

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Half-Dead Colonies Of Montastraea Annularis Release Viable Gametes On A Degraded Reef In The Us Virgin Islands, Ilsa B. Kuffner, T. Don Hickey, Valerie J. Paul, Jennifer M. Morrison, Linda J. Walters, Katie R. Grablow, Teresa Turner, Edward R. Parish Jan 2011

Half-Dead Colonies Of Montastraea Annularis Release Viable Gametes On A Degraded Reef In The Us Virgin Islands, Ilsa B. Kuffner, T. Don Hickey, Valerie J. Paul, Jennifer M. Morrison, Linda J. Walters, Katie R. Grablow, Teresa Turner, Edward R. Parish

Faculty Bibliography 2010s

No abstract provided.

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Characterization Of The Retrocyclin Analogue Rc-101 As A Preventative Of Staphylococcus Aureus Nasal Colonization, Ryan P. Lamers, Colleen R. Eade, Alan J. Waring, Amy L. Cole, Alexander M. Cole Jan 2011

Characterization Of The Retrocyclin Analogue Rc-101 As A Preventative Of Staphylococcus Aureus Nasal Colonization, Ryan P. Lamers, Colleen R. Eade, Alan J. Waring, Amy L. Cole, Alexander M. Cole

Faculty Bibliography 2010s

Nasal colonization of Staphylococcus aureus is a risk factor for pathogenic autoinfection, particularly in postoperative patients and the immunocompromised. As such, standardized preoperative nasal decolonization of S. aureus has become a major consideration for the prevention of nosocomial infection. However, only a few treatment options for nasal decolonization are currently available, with resistance to these approaches already a concern. Here we have identified the macrocyclic theta-defensin analogue RC-101 as a promising anti-S. aureus agent for nasal decolonization. RC-101 exhibits bactericidal effects against S. aureus with the use of in vitro epithelium-free systems, while also preventing the pathogen's proliferation and attachment …

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Evolutionary Analyses Of Staphylococcus Aureus Identify Genetic Relationships Between Nasal Carriage And Clinical Isolates, Ryan P. Lamers, Jason W. Stinnett, Gowrishankar Muthukrishnan, Christopher L. Parkinson, Alexander M. Cole Jan 2011

Evolutionary Analyses Of Staphylococcus Aureus Identify Genetic Relationships Between Nasal Carriage And Clinical Isolates, Ryan P. Lamers, Jason W. Stinnett, Gowrishankar Muthukrishnan, Christopher L. Parkinson, Alexander M. Cole

Faculty Bibliography 2010s

Nasal carriage of Staphylococcus aureus has long been hypothesized to be a major vector for the transmission of virulent strains throughout the community. To address this hypothesis, we have analyzed the relatedness between a cohort of nasal carriage strains and clinical isolates to understand better the genetic conformity therein. To assess the relatedness between nasal carriage and clinical isolates of S. aureus, a genetic association study was conducted using multilocus sequence typing (MLST) and typing of the hypervariable regions of clumping factor and fibronectin binding protein genes. At all loci analyzed, genetic associations between both nasal carriage and clinical isolates …

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Expression And Characterization Of Antimicrobial Peptides Retrocyclin-101 And Protegrin-1 In Chloroplasts To Control Viral And Bacterial Infections, Seung-Bum Lee, Baichuan Li, Shuangxia Jin, Henry Daniell Jan 2011

Expression And Characterization Of Antimicrobial Peptides Retrocyclin-101 And Protegrin-1 In Chloroplasts To Control Viral And Bacterial Infections, Seung-Bum Lee, Baichuan Li, Shuangxia Jin, Henry Daniell

Faculty Bibliography 2010s

Retrocyclin-101 (RC101) and Protegrin-1 (PG1) are two important antimicrobial peptides that can be used as therapeutic agents against bacterial and/or viral infections, especially those caused by the HIV-1 or sexually transmitted bacteria. Because of their antimicrobial activity and complex secondary structures, they have not yet been produced in microbial systems and their chemical synthesis is prohibitively expensive. Therefore, we created chloroplast transformation vectors with the RC101 or PG1 coding sequence, fused with GFP to confer stability, furin or Factor Xa cleavage site to liberate the mature peptide from their fusion proteins and a His-tag to aid in their purification. Stable …

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Abandoning Objectives: Evolution Through The Search For Novelty Alone, Joel Lehman, Kenneth O. Stanley Jan 2011

Abandoning Objectives: Evolution Through The Search For Novelty Alone, Joel Lehman, Kenneth O. Stanley

Faculty Bibliography 2010s

In evolutionary computation, the fitness function normally measures progress toward an objective in the search space, effectively acting as an objective function. Through deception, such objective functions may actually prevent the objective from being reached. While methods exist to mitigate deception, they leave the underlying pathology untreated: Objective functions themselves may actively misdirect search toward dead ends. This paper proposes an approach to circumventing deception that also yields a new perspective on open-ended evolution. Instead of either explicitly seeking an objective or modeling natural evolution to capture open-endedness, the idea is to simply search for behavioral novelty. Even in an …

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Application Of Adaptive Design And Decision Making To A Phase Ii Trial Of A Phosphodiesterase Inhibitor For The Treatment Of Intermittent Claudication, Roger J. Lewis, Jason T. Connor, John R. Teerlink, James R. Murphy, Leslie T. Cooper, William R. Hiatt, Eric P. Brass Jan 2011

Application Of Adaptive Design And Decision Making To A Phase Ii Trial Of A Phosphodiesterase Inhibitor For The Treatment Of Intermittent Claudication, Roger J. Lewis, Jason T. Connor, John R. Teerlink, James R. Murphy, Leslie T. Cooper, William R. Hiatt, Eric P. Brass

Faculty Bibliography 2010s

Background: Claudication secondary to peripheral artery disease (PAD) is associated with substantial functional impairment. Phosphodiesterase (PDE) inhibitors have been shown to increase walking performance in these patients. K-134 is a selective PDE 3 inhibitor being developed as a potential treatment for claudication. The use of K-134, as with other PDE 3 inhibitors, in patients with PAD raises important safety and tolerability concerns, including the induction of cardiac ischemia, tachycardia, and hypotension. We describe the design, oversight, and implementation of an adaptive, phase II, dose-finding trial evaluating K-134 for the treatment of stable, intermittent claudication. Methods: The study design was a …

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Post-Transcriptional Regulation Of Igf1r By Key Micrornas In Long-Lived Mutant Mice, Ruqiang Liang, Amit Khanna, Senthilkumar Muthusamy, Na Li, Harshini Sarojini, John J. Kopchick, Michal M. Masternak, Andrzej Bartke, Eugenia Wang Jan 2011

Post-Transcriptional Regulation Of Igf1r By Key Micrornas In Long-Lived Mutant Mice, Ruqiang Liang, Amit Khanna, Senthilkumar Muthusamy, Na Li, Harshini Sarojini, John J. Kopchick, Michal M. Masternak, Andrzej Bartke, Eugenia Wang

Faculty Bibliography 2010s

Long-lived mutant mice, both Ames dwarf and growth hormone receptor genedisrupted or knockout strains, exhibit heightened cognitive robustness and altered IGF1 signaling in the brain. Here, we report, in both these long-lived mice, that three up-regulated lead microRNAs, miR-470, miR-669b, and miR-681, are involved in posttranscriptional regulation of genes pertinent to growth hormone/IGF1 signaling. All three are most prominently localized in the hippocampus and correspond to reduced expression of key IGF1 signaling genes: IGF1, IGF1R, and PI3 kinase. The decline in these genes expression translates into decreased phosphorylation of downstream molecules AKT and FoxO3a. Cultures transfected with either miR-470, miR-669b, …

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