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Synthesis, Characterization, And Biological Activity Studies Of (E)-N'-((Thiophen-2-Yl)Methylene)Benzofuran-2-Carbohydrazide And Its Metal(Ii) Complexes, Madappa B. Halli, Vijayalaxmi B. Patil, Sumathi R. Bevinamarada Jan 2011

Synthesis, Characterization, And Biological Activity Studies Of (E)-N'-((Thiophen-2-Yl)Methylene)Benzofuran-2-Carbohydrazide And Its Metal(Ii) Complexes, Madappa B. Halli, Vijayalaxmi B. Patil, Sumathi R. Bevinamarada

Turkish Journal of Chemistry

Metal complexes of the type MLX_2, where M = Co(II), Ni(II), Cu(II), Zn(II), Cd(II), and Hg(II), and X = Cl and L = TMBC, Schiff base derived from the condensation of benzofuran-2-carbohydrazide with thiophene-2-aldehyde were synthesized. The structure of the complexes was proposed in light of analytical, IR, UV-Vis, ^1H-NMR, FAB-MS, ESR spectral data, and magnetic studies. The complexes were soluble in DMF and DMSO. The measured molar conductance values indicated that the complexes were nonelectrolytic in nature. On the basis of these studies, 6 coordinated octahedral polymeric structures were assigned to the Co(II), Ni(II), and Cu(II) complexes, and 4 …


Cover And Contents Jan 2011

Cover And Contents

Turkish Journal of Chemistry

No abstract provided.


Analysis Of A Differential Equation Model Of Hiv Infection Of Cd4^+ T-Cells With Saturated Reverse Function, Xiangyun Shi, Gang Li, Xueyong Zhou, Xinyu Song Jan 2011

Analysis Of A Differential Equation Model Of Hiv Infection Of Cd4^+ T-Cells With Saturated Reverse Function, Xiangyun Shi, Gang Li, Xueyong Zhou, Xinyu Song

Turkish Journal of Mathematics

In this paper, an ordinary differential equation model of HIV infection of CD4^+ T-cells with saturated reverse function is studied. We prove that if the basic reproduction number R_0


Synthesis And Evaluation Of The Analgesic Activity Of Some New Isoxazolo[4,5-D]Pyridazin-4(5h)-One Derivatives, Oya Ünsal Tan, Keri̇man Özadali, Özgür Yeşi̇lyurt, Hakan Kayir, İsmai̇l Tayfun Uzbay, Ayla Balkan Jan 2011

Synthesis And Evaluation Of The Analgesic Activity Of Some New Isoxazolo[4,5-D]Pyridazin-4(5h)-One Derivatives, Oya Ünsal Tan, Keri̇man Özadali, Özgür Yeşi̇lyurt, Hakan Kayir, İsmai̇l Tayfun Uzbay, Ayla Balkan

Turkish Journal of Chemistry

Some isoxazolo[4,5-d]pyridazin4(5H)-one derivatives were synthesized and tested for their analgesic activity. The analgesic activities of the compounds were determined by hot-plate and acetic acid writhing tests using morphine and diclofenac as references. Compounds 4a, 4f, 4g, and 4i (25 mg/kg) showed analgesic profiles similar to that of morphine (5 mg/kg).


Synthesis, Crystal Structure, And Antioxidant Properties Of Novel 1,2,4-Triazol-5-Ones Containing 3,4-Dimethoxyphenyl And 3,4-Dihydroxyphenyl Moiety, Yasemi̇n Ünver, Sevgi̇ Meydanal, Kemal Sancak, Di̇lek Ünlüer, Reşat Ustabaş, Esra Düğdü Jan 2011

Synthesis, Crystal Structure, And Antioxidant Properties Of Novel 1,2,4-Triazol-5-Ones Containing 3,4-Dimethoxyphenyl And 3,4-Dihydroxyphenyl Moiety, Yasemi̇n Ünver, Sevgi̇ Meydanal, Kemal Sancak, Di̇lek Ünlüer, Reşat Ustabaş, Esra Düğdü

Turkish Journal of Chemistry

A series of new 4-(3,4-dimethoxyphenethyl)-5-akyl/aryl-2H- 1,2,4-triazol-3(4H)-ones (3a-g) was obtained by the reaction of ethyl 2-(ethoxy)(alkylidene/arylidene)hydrazinecarboxylate (1) and 2-(3,4-dimethoxyphen- yl)ethanamine (2). Compounds 4a-f and 5 were synthesized from the reaction of corresponding compounds 3a-f and 3g with BBr_3, respectively. With elemental analysis, IR, ^1H-NMR, and ^{13C-NMR spectral data, 14 newly synthesized compounds were characterized. The structure of compound 3a was inferred through IR, ^1H- and ^{13}C-NMR, elemental analysis, and X-ray spectral techniques. In addition, the newly synthesized chemicals were screened for their antioxidant properties. Among the chemicals tested, 4a, 4c, 4d, 4f, and 5 exhibited the highest degree of antioxidant activity.


Antioxidant Phenolics From Lobaria Pulmonaria (L.) Hoffm. And Usnea Longissima Ach. Lichen Species, Fadi̇me Atalay, Mesut Bünyami̇ Halici, Ahmet Mavi̇, Ahmet Çakir, Fehmi̇ Odabaşoğlu, Cavi̇t Kazaz, Ali̇ Aslan, Ömer İrfan Küfrevi̇oğlu Jan 2011

Antioxidant Phenolics From Lobaria Pulmonaria (L.) Hoffm. And Usnea Longissima Ach. Lichen Species, Fadi̇me Atalay, Mesut Bünyami̇ Halici, Ahmet Mavi̇, Ahmet Çakir, Fehmi̇ Odabaşoğlu, Cavi̇t Kazaz, Ali̇ Aslan, Ömer İrfan Küfrevi̇oğlu

Turkish Journal of Chemistry

In this study, 1 steroidal and 8 phenolic compounds from Lobaria pulmonaria (L.) Hoffm. and Usnea longissima Ach. were isolated and their chemical structures were characterized by ^1H-NMR, ^{13}C-NMR, DEPT, ^1H-^1H COSY, HMQC, HMBC, IR, UV, and MS spectroscopic methods as stictic acid, isidiophorin, rhizonaldehyde, rhizonyl alcohol, pulmonarianin, vesuvianic acid, ergosterol peroxide, usnic acid, and diffractaic acid. Among these compounds, pulmonarianin, isolated from L. pulmonaria, was characterized for the first time as a natural compound. In addition to isolation and characterization, the lipid peroxidation inhibition and DPPH radical scavenging activities of the isolated compounds were investigated. Among these compounds, isidiophorin, …


Phylogenetic Relationships Between Malcolmia, Strigosella, Zuvanda, And Some Closely Related Genera (Brassicaceae) From Turkey Revealed By Inter-Simple Sequence Repeat Amplification, Beki̇r Doğan, Murat Ünal, Fevzi̇ Özgökçe, Esra Marti̇n, Ayla Kaya Jan 2011

Phylogenetic Relationships Between Malcolmia, Strigosella, Zuvanda, And Some Closely Related Genera (Brassicaceae) From Turkey Revealed By Inter-Simple Sequence Repeat Amplification, Beki̇r Doğan, Murat Ünal, Fevzi̇ Özgökçe, Esra Marti̇n, Ayla Kaya

Turkish Journal of Botany

The genus Malcolmia W.T.Aiton is taxonomically problematic, and some of its species have recently been transferred to the genera Strigosella Boiss. and Zuvanda Dvorák. Three species of this genus are native to Turkey. The revision studies based on molecular data, which were collected in the past few years, display the phylogenetic relations and the systematic positions of the taxa more reliably and apparently. Thus taxonomic problems of species are resolved through DNA-based molecular analyses, which are not affected by environmental factors compared to phenotypic studies. In the present study, the amplifications of the DNA fragments were carried out using ISSR …


Verbascum Ergin-Hamzaoglui (Scrophulariaceae), A New Species From South Anatolia, Turkey, Fai̇k Ahmet Karaveli̇oğullari, Sezgi̇n Çeli̇k, Bi̇rol Başer, Ahter Fi̇şne Jan 2011

Verbascum Ergin-Hamzaoglui (Scrophulariaceae), A New Species From South Anatolia, Turkey, Fai̇k Ahmet Karaveli̇oğullari, Sezgi̇n Çeli̇k, Bi̇rol Başer, Ahter Fi̇şne

Turkish Journal of Botany

Verbascum ergin-hamzaoglui Karavel. sp. nova (Sect. Bothrosperma Murb.), is described for the first time to the scientific community from South Anatolia in Turkey. The diagnostic morphological characters of these new species and other closely related species (V. diversifolium Hochst. and V. cymigerum Hub.-Mor.) are discussed. In addition, distribution maps of V. ergin-hamzaoglui and its related species are provided. The pollen morphologies of 3 taxa belonging to Verbascum L. from the family Scrophulariaceae from Turkey were investigated using a light microscope (LM) and scanning electron microscope (SEM). This family's pollens show similarities to the eurypalynous family. The pollens of these taxa …


Simultaneous Determination Of Copper And Cadmium In Environmental Water And Tea Samples By Adsorptive Stripping Voltammetry, Mohammad Bagher Gholivand, Alireza Pourhossein, Mohsen Shahlaei Jan 2011

Simultaneous Determination Of Copper And Cadmium In Environmental Water And Tea Samples By Adsorptive Stripping Voltammetry, Mohammad Bagher Gholivand, Alireza Pourhossein, Mohsen Shahlaei

Turkish Journal of Chemistry

A sensitive and selective method for the simultaneous determination of copper and cadmium in seawater, river water, and tea samples is described. The method is based on the adsorptive accumulation of 2,2--dithiosalicylic acid complexes of these elements onto a hanging mercury drop electrode, followed by reduction of adsorbed species by voltammetric scan using differential pulse modulation. Optimal analytical conditions were as follows: ligand concentration of 11 nM, pH of 9.5, and an adsorption potential at --300 mV versus Ag/AgCl, with an accumulation time of 60 s. The peak currents are proportional to the concentration of copper and cadmium over the …


The Sorption Of Cadmium(Ii) Ions On Mercerized Rice Husk And Activated Carbon, Mas Rosemal Hakim Mas Haris, Nor Aimi Abdul Wahab, Chong Wan Reng, Baharin Azahari, Kathiresan Sathasivam Jan 2011

The Sorption Of Cadmium(Ii) Ions On Mercerized Rice Husk And Activated Carbon, Mas Rosemal Hakim Mas Haris, Nor Aimi Abdul Wahab, Chong Wan Reng, Baharin Azahari, Kathiresan Sathasivam

Turkish Journal of Chemistry

This work describes the removal of Cd(II) ions in aqueous solution by unmodified and base-modified rice husk (RH) as compared to that by activated carbon (AC). Mercerization of RH was carried out by using dilute solutions (0.013 M) of NaOH, KOH, and Ca(OH)_2. The experiments were conducted in duplicate under 1-batch and 2-batch treatments. In the 1-batch treatment, a fresh 200 mg of each adsorbent was used to study the sorption of the Cd(II) ions from 100-mL solutions at an initial concentration of 5 mg L^{-1}. The adsorption percentage increased in the order of unmodified RH (80.13 \pm 0.46%) < Ca(OH)_2-modified RH (90.74 \pm 0.18%) < NaOH-modified RH (93.36 \pm 0.23%) \le KOH-modified RH (93.78 \pm 0.27%) < AC (98.4 \pm 0.18%). In the 2-batch treatment, a fresh 100 mg of each adsorbent was placed in 100-mL solutions at an initial concentration of 5 mg L^{-1} of Cd(II) ions for 30 min, and the resulting solutions were filtered. The filtrates were subjected to another fresh 100 mg of each adsorbent for an additional 30 min. The percentage removal of the metal ion using the 2-batch treatment for unmodified RH, base-modified RH, and AC was 97.71%, 98.00 \pm 1.00%, and nearly 100%, respectively.


Assessment Of Potential Plastic-Degrading Fungi In Jordanian Habitats, Ibrahim N. Ibrahim, Anwar Maraqa, Khalid M. Hameed, Ismail M. Saadoun, Hamzah M. Maswadeh Jan 2011

Assessment Of Potential Plastic-Degrading Fungi In Jordanian Habitats, Ibrahim N. Ibrahim, Anwar Maraqa, Khalid M. Hameed, Ismail M. Saadoun, Hamzah M. Maswadeh

Turkish Journal of Biology

Out of 70 fungal isolates recovered from soil, wall paints (Latex), and pieces of plastic debris from different habitats in Jordan and plastic shields of street light posts on campus, 35 isolates showed varied potential to degrade polyester-polyurethane (PS-PUR). Six of these isolates (Fusarium solani, Alternaria solani, Spicaria spp., Aspergillus fumigatus, Aspergillus terreus, and Aspergillus flavus) were selected on the basis of their growth rates on basal salt media amended with PS-PUR as the sole source of carbon. Isolates were further evaluated utilizing 3 different methods: 1) direct plating, 2) clear zone in a 2-layered agar media, and 3) liquid …


Isolation, Production, And Characterization Of An Extracellular Lipase From Trichoderma Harzianum Isolated From Soil, Serdar Ülker, Arzu Özel, Ahmet Çolak, Şengül Alpay Karaoğlu Jan 2011

Isolation, Production, And Characterization Of An Extracellular Lipase From Trichoderma Harzianum Isolated From Soil, Serdar Ülker, Arzu Özel, Ahmet Çolak, Şengül Alpay Karaoğlu

Turkish Journal of Biology

This is the first report about the characterization of Trichoderma harzianum lipase. A novel strain of Trichoderma harzianum IDM14D was isolated from soil. The isolated strain was cultivated for lipase production in shake flasks at 30 °C for 7 days. For lipase production, it was determined that the best carbon source was glucose and the best nitrogen source was peptone. Maximum biomass was produced at a concentration of 1.25 g/L, in 7 days. The optimum pH and temperature for activity of the enzyme were 8.5 and 40 °C, respectively. The lipase was stable at a pH range of 8.0-10.0 and …


Acid Proteinase Enzyme Activity In Candida Albicans Strains: A Comparison Of Spectrophotometry And Plate Methods, Sevi̇m Akçağlar, Beyza Ener, Okan Töre Jan 2011

Acid Proteinase Enzyme Activity In Candida Albicans Strains: A Comparison Of Spectrophotometry And Plate Methods, Sevi̇m Akçağlar, Beyza Ener, Okan Töre

Turkish Journal of Biology

In recent years, the incidence of fungal infections has been rising all over the world. Although the amount of research in the field of pathogenic fungi has also increased, there is still a need for studies on fungal virulence. In this investigation, we focused on testing bloodstream-identified Candida albicans in experimental in vitro virulence assays. The proteinase enzyme activities of 30 C. albicans strains isolated from the bloodstream and sterile body fluids were investigated (15 colonizations, 15 infections). The spectrophotometric and plate methods were used to determine proteinase activity. The correlations between the 2 methods were compared. This study indicates …


Isolation And Characterization Of Xylanolytic New Strains Of Anoxybacillus From Some Hot Springs In Turkey, Kadri̇ye İnan, Sabri̇ye Çanakçi, Ali̇ Osman Beldüz Jan 2011

Isolation And Characterization Of Xylanolytic New Strains Of Anoxybacillus From Some Hot Springs In Turkey, Kadri̇ye İnan, Sabri̇ye Çanakçi, Ali̇ Osman Beldüz

Turkish Journal of Biology

Some hot springs located in the west of Turkey were investigated regarding the presence of xylanolytic thermophilic microorganisms. Based on phenotyping characteristics and 16S rRNA gene sequence analysis, 9 of these xylanolytic isolates belonged to the genus Anoxybacillus, and grew optimally at about 50-60 °C on nutrient agar. The 16S rRNA gene sequence analysis showed that these isolates resembled the Anoxybacillus species =>97 and these isolates are members of the genus Anoxybacillus. Based on the DNA-DNA hybridization study, SDS-PAGE profile, and biochemical and physiological features, I3, CT1Sari, and BT2.1 isolates are new strains of Anoxybacillus gonensis; I4.2 and B9.3 …


In Vitro Propagation Of Diyarbakır Watermelons And Comparison Of Direct-Seeded And Transplanted Watermelon, Veysi̇ Okumuş, Vedat Pi̇ri̇nç, Ahmet Onay, Davut Başaran Jan 2011

In Vitro Propagation Of Diyarbakır Watermelons And Comparison Of Direct-Seeded And Transplanted Watermelon, Veysi̇ Okumuş, Vedat Pi̇ri̇nç, Ahmet Onay, Davut Başaran

Turkish Journal of Biology

A rapid protocol using shoot tip explants for micropropagation of Diyarbakır watermelon [Citrullus lanatus (Thunb.) Matsum. & Nakai] types Sürme, Beyazkış and Karakış was achieved. Shoot tips from 5-day-old in vitro germinated seedlings were cultured on shoot regeneration medium for 3 weeks. The effects of the different concentrations of benzyl adenine (BA) and carbohydrate types on shoot proliferation were examined. The results confirmed that a range of 0.5-1.0 mg/L of BA was almost equally effective in promoting the shoot length of cultures in the 3 genotypes. Sucrose, in the presence of BA, was superior to other carbohydrates for the 3 …


Characterization Of Poly Phenol Oxidase In Two In Vitro Regenerated Cultivars Of Mucuna: Mucuna Pruriens L. And Mucuna Prurita H., Raghavendra Sathyanarayana, Vadlapudi Kumar, Chapeyil Kumaran Ramesh, Mahadevappa Parmesha, Mahaboob Habeebulla Moinuddin Khan Jan 2011

Characterization Of Poly Phenol Oxidase In Two In Vitro Regenerated Cultivars Of Mucuna: Mucuna Pruriens L. And Mucuna Prurita H., Raghavendra Sathyanarayana, Vadlapudi Kumar, Chapeyil Kumaran Ramesh, Mahadevappa Parmesha, Mahaboob Habeebulla Moinuddin Khan

Turkish Journal of Biology

Polyphenol oxidases (PPOs EC 1.14.18.1) were isolated from Mucuna pruriens and Mucuna prurita and confirmed as tyrosinases involved in L-DOPA production. PPOs were extracted by using a 0.05 M phosphate buffer, pH 7.0. The purified enzyme was resolved into a single band by PAGE, the enzyme was confirmed as PPO by activity staining, and SDS-PAGE analysis revealed that the purified PPO of both the species is a tetramer. Substrate specificity experiments were carried out with catechol, L-DOPA, L-tyrosine, and p-cresol. Of these, catechol was evaluated as the most suitable substrate based on the determined K_{m} and V_{max} values. The optimum …


Antioxidant Capacity Of Juice From Different Papaya (Carica Papaya L.) Cultivars Grown Under Greenhouse Conditions In Turkey, Aysun Özkan, Hami̇de Gübbük, Esma Güneş, Ayşe Erdoğan Jan 2011

Antioxidant Capacity Of Juice From Different Papaya (Carica Papaya L.) Cultivars Grown Under Greenhouse Conditions In Turkey, Aysun Özkan, Hami̇de Gübbük, Esma Güneş, Ayşe Erdoğan

Turkish Journal of Biology

The fruits of Carica papaya L. (Caricaceae) are valuable as food and are also used in traditional medicine. The present study was designed to assess the antioxidant potential of the juices of 3 papaya cultivars (PCJ): Sunrise Solo, Red Lady, and Tainung. The antioxidant capacity of PCJ obtained from fully ripened fruit was determined by the following methods: scavenging of the free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH), reducing power assay, scavenging of superoxide radicals, 2-deoxyribose oxidation assay, and thiocyanate assay. Total phenolic content (TPC) of PCJ was determined by the Folin-Ciocalteu method. In the DPPH assay (IC_{50} values), the effect of Sunrise …


Chemical Composition And Nutritional Value Of A Wild Edible Ectomycorrhizal Mushroom, Tricholoma Anatolicum, Erbi̇l Kalmiş, Hasan Yildiz, Bülent Ergönül, Fati̇h Kalyoncu, Mehmet Hali̇l Solak Jan 2011

Chemical Composition And Nutritional Value Of A Wild Edible Ectomycorrhizal Mushroom, Tricholoma Anatolicum, Erbi̇l Kalmiş, Hasan Yildiz, Bülent Ergönül, Fati̇h Kalyoncu, Mehmet Hali̇l Solak

Turkish Journal of Biology

The chemical composition and nutritional value of a wild edible ectomycorrhizal mushroom from southwestern Anatolia, Tricholoma anatolicum, were analyzed. Moisture, crude oil, protein, ash, total carbohydrate content, and mineral composition of the mushrooms studied, including Fe, Na, K, Zn, Cu, Ca, Cd, and Pb, were determined. The energy values of the samples were also calculated. The analyses were conducted during the 3 different growing stages of the mushrooms: mycelium, young fruiting bodies, and mature fruiting bodies. The highest values for moisture and crude oil contents were found to be in the mycelium, ash, and carbohydrate content of young fruiting bodies. …


Biological Characterization Of Iranian Walnut (Juglans Regia) Leaves, Reza Sharafati Chaleshtori, Farhad Sharafati Chaleshtori, Mahmoud Rafieian Jan 2011

Biological Characterization Of Iranian Walnut (Juglans Regia) Leaves, Reza Sharafati Chaleshtori, Farhad Sharafati Chaleshtori, Mahmoud Rafieian

Turkish Journal of Biology

In recent years, emphasis has been placed on the use of natural materials in the control and treatment of various infections, as some chemically synthesized drugs have undesirable side effects. In this study, the ethanol extract of Iranian walnut leaves was examined for antibacterial activities on Streptococcus mutans, Streptococcus salivarius, Streptococcus sanguinis, and Actinomyces viscosus using the microdilution method. Total phenols, flavonoids, and flavonols were also determined colorimetrically. The minimum inhibitory concentrations (MIC) for ethanolic extract ranged between 15.6 and 187.5 mg/mL and minimum bactericidal concentrations (MBC) ranged between 31.25 and 250 mg/mL. Total phenols were 410 ± 14.43 mg/g …


Determination Of Volatile Compounds Of Pine Honeys, Si̇bel Si̇li̇ci̇ Jan 2011

Determination Of Volatile Compounds Of Pine Honeys, Si̇bel Si̇li̇ci̇

Turkish Journal of Biology

The volatile compounds of 13 Turkish pine (Pinus brutia Ten.) honey samples were characterized by solid phase microextraction (SPME) analysis, followed by gas chromatography-mass spectrometry (GC-MS) analysis. A total of 42 volatile compounds were identified, but volatile compounds such as nonana1, benzene, 4-hexen-3-ol, alpha-pinene, and 2-heptanone were recognized to be specific floral origin markers of the pine honey. The SPME extraction method was proposed as an alternative way to carry out pollen analysis for floral source detection, especially for products such as pine honey, by characterization of honeydew elements.


Cover And Contents Jan 2011

Cover And Contents

Turkish Journal of Biology

No abstract provided.


Cover And Contents Jan 2011

Cover And Contents

Turkish Journal of Biology

No abstract provided.


C- And Nor Stained Karyotypes Of Mole Rat, Nannospalax Xanthodon (2n = 54) From Kırıkkale, Turkey, Ati̇lla Arslan, Kubi̇lay Toyran, Serdar Gözütok, Tarkan Yorulmaz Jan 2011

C- And Nor Stained Karyotypes Of Mole Rat, Nannospalax Xanthodon (2n = 54) From Kırıkkale, Turkey, Ati̇lla Arslan, Kubi̇lay Toyran, Serdar Gözütok, Tarkan Yorulmaz

Turkish Journal of Biology

In the present study, the 2n = 54 chromosomal race of blind mole rats, Nannospalax xanthodon superspecies, from Kırıkkale Province in Turkey was investigated. Conventional chromosome staining, Ag-NOR (Nucleolus Organizer Region) staining, and C-banding analysis were carried out on specimens of mole rats. The karyotype including 3 metacentric pairs (nos. 1-3), 3 submetacentric pairs (nos. 4-6), 3 subtelocentric pairs (nos. 7-9), and 17 acrocentric pairs (nos. 10-26) of autosomes (NFa = 70). C-heterochromatin regions were found in the centromeric and pericentromeric region and the short arms of some bi-armed autosomal pairs, and C-heterochromatin was localized in pericentromeric areas of a …


Cytogenetic And Biochemical Effects Of Imazethapyr In Wheat (Triticum Durum), Manijeh Hoseiny Rad, Ali Ashraf Aivazi, Shobha Jagannath Jan 2011

Cytogenetic And Biochemical Effects Of Imazethapyr In Wheat (Triticum Durum), Manijeh Hoseiny Rad, Ali Ashraf Aivazi, Shobha Jagannath

Turkish Journal of Biology

Mitotic abnormalities, chromosomal behavior, and protein content in wheat (Triticum durum) due to imazethapyr (IM) treatment were determined. Wheat seeds (variety HI-8663) were exposed to 5 IM concentrations (0.5, 0.89, 1.58, 2.81, and 5 ppm) according to the between paper method, in laboratory conditions. The protein content in the root-shoot axis and endosperm decreased significantly with the dosage increase. Cytological observations showed that the mitotic frequency in the root meristematic cells decreased parallel to the increase in concentrations. The most common types of the observed anomalies were sticky chromosomes, lagging chromosomes, scattered chromosomes, chromatin bridges, and micronuclei. The results revealed …


Investigation Of Legionella Pneumophila And Free Living Amoebas In The Domestic Hot Water Systems In İstanbul, Di̇lara Meryem Burak, Zuhal Zeybek Jan 2011

Investigation Of Legionella Pneumophila And Free Living Amoebas In The Domestic Hot Water Systems In İstanbul, Di̇lara Meryem Burak, Zuhal Zeybek

Turkish Journal of Biology

Legionella pneumophila (L. pneumophila), known to cause legionnaires' disease, has been found in various aquatic environments and man-made water systems. Free living amoebas (FLAs) live in the same environments and can cause infections such as encephalitis, keratitis. Moreover, FLAs play a major role in L. pneumophila transmission. In the present study, the presence of L. pneumophila and FLAs was examined in water and swab samples obtained from shower heads (hot water) in 61 houses in İstanbul. Nonnutrient agar (NNA) was used for isolation of FLAs in all samples and buffered charcoal yeast extract (BCYE) agar with addition of glycine, vancomycine, …


Detection Of Prokaryotic Microbial Communities Of Çamaltı Saltern, Turkey, By Fluorescein In Situ Hybridization And Real-Time Pcr, Mehmet Burçi̇n Mutlu, Kiymet Güven Jan 2011

Detection Of Prokaryotic Microbial Communities Of Çamaltı Saltern, Turkey, By Fluorescein In Situ Hybridization And Real-Time Pcr, Mehmet Burçi̇n Mutlu, Kiymet Güven

Turkish Journal of Biology

Knowledge of the functions, interactions, and diversity of extremely halophilic microorganisms mostly comes from the results of studies performed in different salterns throughout the world. As model habitats, salterns allow the comparison of different techniques used for qualitative and quantitative analysis of halophilic communities in these hypersaline environments. Çamaltı Saltern is the biggest coastal solar saltern located on the Aegean coast of Turkey, and it produces most of the salt consumed in the country. In the present study, detection of prokaryotic communities of the Çamaltı Saltern was performed using 2 culture-independent methods. Real-time polymerase chain reaction (RT-PCR) and fluorescein in …


Production, Purification, And Characterization Of \Alpha-Amylase By Bacillus Subtilis And Its Mutant Derivates, Eli̇f Demi̇rkan Jan 2011

Production, Purification, And Characterization Of \Alpha-Amylase By Bacillus Subtilis And Its Mutant Derivates, Eli̇f Demi̇rkan

Turkish Journal of Biology

The effects of various carbon and nitrogen sources on production of \alpha-amylase by Bacillus subtilis and its mutant derivates were investigated. The maximum production of \alpha-amylase by all strains was obtained in the presence of mesoinositol as the carbon source. There was no more significant increase in enzyme yield in the case of the supplementation of nitrogen sources, whereas malt extract and tryptone were preferred nitrogen sources for amylase production by Bacillus subtilis and mutant U 2-6 strain, respectively. \alpha-Amylases of B. subtilis and its mutant strain (EBUE 5-3) were purified through a series of steps, and characterized. The optimum …


Utilization Of Cytochrome Oxidase I In Cephus Pygmeus (L.) (Hymenoptera: Cephidae), Ertan Mahi̇r Korkmaz, Mahi̇r Budak, Hasan Hüseyi̇n Başibüyük Jan 2011

Utilization Of Cytochrome Oxidase I In Cephus Pygmeus (L.) (Hymenoptera: Cephidae), Ertan Mahi̇r Korkmaz, Mahi̇r Budak, Hasan Hüseyi̇n Başibüyük

Turkish Journal of Biology

The mitochondrial cytochrome oxidase I (COI) gene region of Cephus pygmeus (L.) (Hymenoptera: Cephidae) was characterized at the nucleotide and amino acid levels. The partial sequence (687 bp) of the gene was determined and compared with other cephids and insect species at both the nucleic acid and amino acid sequence levels. The examined fragment was placed at an equal distance to both ends of the gene and consisted of 13 structural regions. The 5' end of the partial COI gene was more informative than the 3' end at the intraspecific level. Pairwise sequence divergences ranged from 0.15% to 1.17% at …


The Effects Of Leptin Hormone On Locomotor Activity In Syrian Hamsters (Mesocricetus Auratus), Bülent Gündüz, Alper Karakaş Jan 2011

The Effects Of Leptin Hormone On Locomotor Activity In Syrian Hamsters (Mesocricetus Auratus), Bülent Gündüz, Alper Karakaş

Turkish Journal of Biology

The suprachiasmatic nucleus (SCN) generates and controls the circadian rhythms in mammals including the rhythm of locomotor activity. Leptin is a hormone secreted by adipose tissue that informs the brain about the fat stores. SCN neurons express leptin receptors. Here we investigated the effects of 3 different leptin administrations on the locomotor activity of the Syrian hamsters maintained in constant darkness. Animals were intraperitoneally (ip) injected (4 µg/kg), subcutaneously (sc) infused (4 µg/kg), or intra-SCN infused (0.4 µg/kg) with leptin for 3 days at circadian time 10 whereas the controls received saline (0.9% NaCl) at the same time in order …


A Comparative Evaluation Of Antioxidant And Anticancer Activity Of Essential Oil From Origanum Onites (Lamiaceae) And Its Two Major Phenolic Components, Aysun Özkan, Ayşe Erdoğan Jan 2011

A Comparative Evaluation Of Antioxidant And Anticancer Activity Of Essential Oil From Origanum Onites (Lamiaceae) And Its Two Major Phenolic Components, Aysun Özkan, Ayşe Erdoğan

Turkish Journal of Biology

The objectives of the present study were to determine the effects of essential oil from Turkish oregano (Origanum onites L.) and its 2 major phenolic components, carvacrol and thymol, on cell viability, and to investigate their cytoprotective (antioxidant) effects against hydrogen peroxide-induced cytotoxicity and membrane damage in hepatoma G2 (Hep G2) cells. Their antioxidant properties were also evaluated with 2 in vitro complementary test systems: DPPH radical scavenging activity and linoleic acid oxidation inhibition. The aerial parts of the essential oil were isolated using a Clevenger-type apparatus (hydrodistillation) and its components were analyzed by gas chromatography/mass spectrometry (GC/MS). The essential …