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Complete Genome Analyses Of A Novel Narlivirus Identified In The Ectomycorrhizal Fungus Amanita Vidua, Ilgaz Akata, Eda Kumru, Gülce Edi̇ş, Ertuğrul Kaya, Ergi̇n Şahi̇n

Turkish Journal of Botany

The widespread utilisation of deep sequencing has dramatically enhanced and revolutionised our understanding of virus diversity, particularly in complex environments such as soil and biological samples. This research explored mycovirus sequence analyses in the ectomycorrhizal fungus Amanita vidua, utilising deep sequencing alongside total double-stranded RNA (dsRNA) extractions. Our investigation unveiled a unique member of the proposed family, Narliviridae, designated as Amanita vidua narlivirus 1 (AvNV1), in an A. vidua specimen. AvNV1, classified under Amabiliviricetes, possesses a distinct genome structure and sequence attributes. T horough analyses indicated that AvNV1 exhibits sequence similarities with other mycoviruses from the proposed family Narliviridae and …

Unveiling The Physical Interaction Between Gsnor1 And Trx-H Isoforms, Anika Tabassum, Gary J. Loake

Turkish Journal of Botany

This study aims to investigate any possible interaction between two highly conserved regulators of cellular SNO levels: S-nitrosoglutathione reductase (GSNOR1) and thioredoxin (TRX). These two enzymes control global S-nitrosylation levels and thus impact the cellular redox state during immune responses. A mutant line (Δtrx1trx2sfa1) of Saccharomyces cerevisiae MaV203 strain was generated to explore this possibility rather than using a classical strain. We identified and characterized the physical interaction between GSNOR1 and Arabidopsis cytosolic h isoforms in a yeast two-hybrid screen. Out of the seven TRX isoforms, AtGSNOR1 displayed a high interaction with AtTRXh3, AtTRXh4, and AtTRXh5 (AtTRXh5>AtTRXh4 & AtTRXh3). …

A New Species From Türkiye: Erigeron Akaydinii (Asteraceae), Batikan Günal, Gürkan Semi̇z, Sultan Akyol

Turkish Journal of Botany

Erigeron akaydinii Günal & Semiz sp. nov., belonging to the Trimorpha Cass. (DC) section (Asteraceae), is described as a new species from Uludağ Mountain (Bursa, Türkiye). This study examines the diagnostic traits of E. akaydinii and related species, including morphological, palynological, and molecular analyses. Notes on the ecology, distribution, and conservation status of the new species are also provided, along with a diagnostic key for relevant taxa.

Taxonomic Revision Of Korean Salix Section Subviminales Based On Comparative Morphology And Complete Plastome Analysis, Young Moon Kim, Shukherdorj Baasanmunkh, Nudkhuu Nyamgerel, Won Seok Lee, Dong Chan Son, In Kyu Park, Bom Bi Jin, Hyo Sig Won, Hyeok Jae Choi

Turkish Journal of Botany

Salix section Subviminales, including only three species, is endemic to East Asia. In this study, we revised three species, S. gracilistyla, S. graciliglans, and S. blinii in Korea based on their plastomes and extensive morphological analyses to provide a comprehensive understanding of their taxonomic relationships. Based on our findings, we identified the morphological characteristics of S. blinii endemic to Korea, including its small size and distinct leaf shapes, which are well distinguished from both S. gracilistyla and S. graciliglans. Most of the morphological characteristics of S. gracilistyla and S. graciliglans overlapped, particularly leaf shape, floral structure, and growth habits. Next, …

On The Images Of N-Ellipses Under The Möbius Transformations, Ni̇hal Özgür

Turkish Journal of Mathematics

In this paper, we examine the images of n-ellipses under the Möbius transformations (linear fractional transformations) for n ≥ 3. As a result of this investigation, we present a new and generalized version (called n-generalized Apollonius circle) of the concept of an Apollonius circle in the plane.

Lancret-Type Results For A New Family Of Curves In Minkowski 3-Space, Héctor Guerrero Mora, Willy Sierra

Turkish Journal of Mathematics

In this article, using the concept of a Killing vector field along a curve, we extend certain results concerning a family of curves—previously studied in three-dimensional Euclidean space—to the setting of three-dimensional Minkowski space. Depending on the causal character of the curve, we derive Lancret-type results that establish the necessary and sufficient conditions for the existence of this family in three-dimensional Minkowski space. We determine the intrinsic equation of this family in three cases: (i) when the curve is nonnull with a nonnull acceleration vector, (ii) when the curve is spacelike with a null acceleration vector, and (iii) when the …

Cover And Contents

Turkish Journal of Biology

No abstract provided.

A Tetracycline-Inducible Split Turboid System For Specific Biotinylation And Identification Of Nuclear Proteins From Hek293t Cells, Mehmet Sarihan, Fatma Zehra Özen, Murat Kasap, Gürler Akpinar

Turkish Journal of Biology

Background/aim: To overcome the limitations of conventional organelle isolation methods including low purity, low yield, sample degradation, scalability and the need for multiple centrifugation steps, an improved nuclear protein enrichment approach was developed using the modified Split TurboID biotin ligase enzyme.

Materials and methods: A construct was created in which the N-terminal domain of TurboID, fused to the FK506-binding protein (FKBP) was targeted to the nucleus. This construct was incorporated into a tetracycline-inducible gene expression vector. Similarly, the C-terminal domain of TurboID was fused to the rapamycin-binding domain of mTOR (FRB) and directed to the nucleus. This construct …

Prooxidant Effect Of Uric Acid On Human Leukocytic Dna: An In Vitro And Ex Vivo Study, Yim Tong Savio Szeto, Vincy Sze Wing Li, Yuen Lam Pon

Turkish Journal of Biology

Background/aim: Uric acid is a major contributor to the total antioxidant capacity of human plasma. However, this endogenous substance’s antioxidant and prooxidant properties have not yet been reported.

Conclusion: In this study, the comet assay was employed in vitro to determine the effect of uric acid on DNA damage in human lymphocytes and leukocytic DNA damage in hyperuricemia patients with and without renal failure.

Results: DNA damage in lymphocytes occurred at uric acid concentrations of ≥600 μM. Adding catalase to the uric acid solution diminished the damaging effect, indicating that hydrogen peroxide mediated the prooxidant activity. Moreover, …

Intrinsic Apoptotic Effect Of Anatolian Honeybee (Apis Mellifera Anatoliaca) Venom Promoted With Mesoporous Silica Nanocarriers, Batuhan Orman, Ayli̇n Koç, Di̇dem Şen Karaman, Ayşe Nalbantsoy

Turkish Journal of Biology

Background/aim: The use of bee products or treatment with bees, as a complement to conventional medicine is attracting considerable attention in cancer research. Although discoveries related to the potential anticancer effects of bee venom are increasing, the unstable nature of venom biomolecules remains a limiting factor for their usage. In this study, we employed mesoporous silica nanocarriers (MSNs) to provide precise dosing and prevent carriers from biomolecule degradation thanks to the outstanding loading capacity provided by the pores, excellent chemical and biological robustness, and ability to improve bioavailability.

Materials and methods: MSNs were synthesized and physicochemical characterizations were …

N-Cadherin Mimetic Hydrogels Drive Superior Regenerative And Paracrine Responses In 3d Cultures Of Adipose-Derived Mesenchymal Stem Cells, Demet Kaçaroğlu, Alper Murat Ulaşli, Aybüke Di̇denur Sak, Seher Yaylaci

Turkish Journal of Biology

Background: Cadherin-based biomaterials play a pivotal role in influencing the fate of mesenchymal stem cells (MSC). Enhancing the adhesion of adipose tissue-derived MSCs has been shown to augment their paracrine effects while N-cadherin biomaterials have been suggested to regulate the paracrine effects of MSCs via specific growth factors although the precise mechanisms underlying this regulation remain insufficiently understood. This study aims to compare the effects of a 3D N-cadherin mimetic environment on cell viability, apoptosis, extracellular matrix regulation, and growth factor expression with those observed in traditional 2D and 3D spheroid cultures. Additionally, the study seeks to evaluate the …

Apigenin Attenuates Ischemia–Reperfusion-Induced Pulmonary Ferroptosis And Fibrosis By Activating The Nrf2/Ho-1/Gpx4 Axis In Mice, Liang Zhang, Haojie Li, Shichen Xu, Hao Wen, Chaoxiao Yu

Turkish Journal of Biology

Background/aim: Acute lung injury (ALI) is a major cause of morbidity and mortality after lung ischemia-reperfusion injury (LIRI). In recent years, pulmonary ferroptosis and its associated fibrosis have been recognized as important causes of LIRI. The purpose of this study is to investigate apigenin (APG) as a potential therapeutic target for treating LIRI-induced pulmonary ferroptosis and fibrosis.

Materials and methods: A rat model of LIRI was established and the rats were randomly divided into three groups, a sham group, a LIRI group, and an APG group. The pathological changes of the lung tissue were evaluated using hematoxylin-eosin staining and …

Progression Of Electrophysiological Impairments In Diabetic Cardiomyopathy And Intervention Using Enicostemma Axillare, Victor Arokia Doss, Dharaniyambigai Kuberapandian

Turkish Journal of Biology

Earlier studies widely reported advanced ventricular impairments only at the later stages of diabetic cardiomyopathy (DCM). The present study aimed to understand the duration-based early to late stages of cardiac electrophysiological impairments using electrocardiography (ECG) and intervention using Enicostema axillare subsp. littorale. The experimental rats were streptozotocin (SZ)-induced diabetic rats that were administered E. axillare formulations as follows: (1) normal; (2) SZ - 40 mg/kg, single, i.p.); (3) SZ + insulin (2 U/day) + losartan (10 mg/kg); (4) SZ + insulin + losartan + E. axillare decoction (2 mL/day); (5) SZ + E. axillare decoction; (6) SZ + E. axillare …

Resveratrol Modulates Triosephosphate Isomerase And Mineralization In Osteosarcoma Cells: Potential Target For Novel Therapeutic Strategies, Gonca Tuna, Si̇bel Çinar Asa, Eli̇f Ertürk, Yaren Yildiz, Ferda Ari

Turkish Journal of Biology

Background/aim: Osteosarcoma, a primary malignant bone tumor, is challenging to treat due to its aggressive nature and limited therapies. Resveratrol (RES), a natural polyphenol, has potential anticancer properties. Hence, we investigated RES’s impact on osteosarcoma cells, focusing on triosephosphate isomerase (TPI) and related mechanisms.

Materials and methods: RES was applied to osteosarcoma (SaOS-2) and healthy fetal osteoblast (hFOB 1.19) cells for 48 h, and cell viability was measured by SRB assay. The mode of cell death was examined using Hoechst 33342/annexin V/propidium iodide. TPI and methylglyoxal (MG) enzyme levels were determined by ELISA. The effect of RES on …

Knockdown Of Plexin C1 Induces Epithelial-To-Mesenchymal Transition And Confers Resistance To Multikinase Inhibitors In Hepatocellular Carcinoma Cells, Gamze Güngör Topcu, Arzu Aysan, Şevval Di̇k, Mai̇de Şeker, Meli̇ke Bi̇nnur Bahçekapili, Sude Topkaraoğlu, Di̇lek Yavuzer, Tamer Yağci

Turkish Journal of Biology

Background/aim: HCC is a common and lethal malignancy and multi-kinase inhibitors (MKIs) are among the therapeutic options for unresectable cases. However, response rates to MKIs remained variable, necessitating the identification of predictive biomarkers. Plexin C1 (PLXNC1), a receptor involved in cell signaling, has emerged as a potential candidate to regulate tumor responses. This study aims to evaluate the impact of PLXNC1 expression on the sensitivity of HCC cells to MKI therapy.

Materials and methods: shRNA-mediated PLXNC1 knock-down and control clones of HCC cell lines PLC/PRF/5 and Hep3B were generated, and downregulation of PLXNC1 was confirmed using Western blotting. …

Investigation Of The Interaction Conditions Of As2s5 And Cucl2 Compounds In Water And Ethylene Glycol Medium, Huseyn Imanov, Mahnur Jafarli

Turkish Journal of Chemistry

The article presents the results of interaction conditions of CuCl2 and As2S5 in ethylene glycol and aquatic medium. Besides, results of the obtained samples' physicochemical properties are given by X-ray diffraction (XRD), differential thermogravimetric (DTG), thermogravimetric (TG), elemental and scanning electron microscope (SEM) methods. It was found that when ethylene glycol and water are used as a solvent and CuCl2 and As2S5 as the initial components, Cu3AsS4 is obtained at a temperature of 323-353 K. According to the results of DTA analysis, it was found that the melting point of obtained samples was 964.4 K contrary to the reactions carried …

Synthesis Of Thiazole-Integrated Pyrrolotriazinones: Evaluations Of Cytotoxicity And Effects On Pi3k Levels In Cancer Cells, Eylem Kuzu, Ege Arzuk, Fuat Karakuş, Burak Kuzu, Hasan Genç

Turkish Journal of Chemistry

The synthesis of novel heterocyclic compounds, particularly those targeting critical signaling pathways in cancer, represents a promising approach to drug development. In this study, we designed and synthesized a series of thiazole-integrated pyrrolotriazinone derivatives, aiming to combine the antiproliferative properties of thiazole with the PI3K inhibitory activity of pyrrolotriazinones. The PI3K pathway, which plays a critical role in regulating cell growth, proliferation, and survival, is frequently dysregulated in cancer, making it an attractive target for therapeutic intervention. The synthesized derivatives were evaluated for their cytotoxic activities against MCF-7, A549, and HepG2 cancer cell lines. Their effect on PI3K protein levels …

A New Turn Off Fluorescent Nir Probe For Hypochlorous Acid And Its Applications, Ayten Ibrahimova, Ayşe Nur Önem, Mehmet Altun, Emi̇n Ahmet Yeşi̇l, Asli Baysal, Hasan Saygin, Ahu Soyocak, Mustafa Özyürek

Turkish Journal of Chemistry

Hypochlorous acid (HOCl) is a potent nonradical oxidant involved in various physiological processes, particularly within the human immune system. In this study, we introduce a novel, rapid, and highly efficient fluorometric method for the detection of HOCl. The method utilizes a near-infrared (NIR)-based fluorescent probe, NIR-QBH, which is characterized by its high sensitivity and chemical stability. NIR-QBH, containing olefinic C=C bonds, exhibits strong NIR emission at 660 nm (λex = 618 nm). The detection mechanism relies on the oxidation of the C=C bond in the NIR-QBH structure by HOCl, resulting in the formation of non-fluorescent products. With a detection limit …

Ecofriendly Synthesis And Characterization Of Oxygen-Enriched G-C3n4 From Diverse Precursors For Efficient Organic Dye Decontamination, Muchammad Tamyiz

Turkish Journal of Chemistry

Industrial wastewater from sectors such as textiles, printing, and pharmaceuticals contains harmful pollutants, including nonbiodegradable dyes, which pose significant challenges for environmental safety. Neutral red, a cationic dye commonly found in wastewater, obstructs photosynthesis in aquatic ecosystems and carries potential toxicity. Traditional methods of dye removal often prove ineffective due to the chemical stability of these compounds. In this study, oxygen-doped graphitic carbon nitride (O-doped g-C3N4) was synthesized as an innovative photocatalyst for the degradation of neutral red dye under visible light. The material was synthesized through a sustainable process involving the calcination of urea, dicyandiamide, and oxalic acid, and …

Fabrication Of Au–Pd Bimetallic Dendrites And Their Catalytic Application For4-Nitrophenol Reduction, Jun Liu, Xuanyan Liu, Hongguang Liao

Turkish Journal of Chemistry

Bimetal nanocatalysts hold significant application potential in catalytic reactions due to their compositions, morphologies, and electronic structures. In the present work, a series of AuPd alloy dendrites were successfully prepared using sodium formate, without surfactants or templates. A systematic investigation was then conducted to analyze the composition of the AuPd bimetallic dendrites and the effects of preparation conditions on their structure and catalytic performance. The AuPd alloy dendrites were characterized through various techniques, including scanning electron microscopy, transmission electron microscopy, X-ray energy scattering spectroscopy, X-ray diffraction, and X-ray photoelectron spectroscopy. The results showed that the Au:Pd ratio in the AuPd …

Sustainable Forage Yield Improvement Through Perennial Grass–Legume Intercropping In Degraded Grasslands, Alina-Ștefania Stanciu

Turkish Journal of Agriculture and Forestry

Grasslands constitute one of the largest terrestrial biomes, providing essential ecosystem services such as biodiversity preservation, food production, and climate change mitigation. Globally, the desertification and degradation of grasslands have been accelerated by intensified land use and climate change. It is crucial to conserve and restore grasslands to address climate and biodiversity challenges. However, due to their diverse nature and undervalued benefits for humans, grasslands have often been overlooked in terms of preservation, conservation, and restoration compared to forests. In sustainable agriculture, intercropping grasses with legumes can enhance crop nutritional quality, increase productivity, and impact soil microbiological activity, ultimately leading …

Effect Of Different Mulch Materials Applications On Weed Control, Plant Characteristics, And Yield In Garlic (Allium Sativum L.), Ayşe Usanmaz Bozhüyük

Turkish Journal of Agriculture and Forestry

This study was conducted in plots under controlled conditions in the Silvan district of Diyarbakır Province in 2023. The local garlic genotype (Allium sativum L.) from Diyarbakır was used as the plant material. Three different organic mulching materials (sawdust, pine, and straw) were applied at a thickness of 5 cm. The effects of these mulching materials on leaf number, root length, plant height, number of cloves per plant, plant density, bulb diameter, dry and fresh weight of garlic, dry and fresh weight of weeds, weed density, and yield were analysed. A total of 34 weed species from 15 families were …

Appraisal Of Germination Behavior And Activity In Newly Developed Rice Lines, Muhammad Usman Akbar, Muhammad Aqeel, Maryam M. Alomran, Ijaz Haider, Omar Mahmoud Al-Zoubi, Ali Noman

Turkish Journal of Agriculture and Forestry

Rice is a fundamental food source for more than half of the global population. This study evaluated the germination capacity of four rice lines [Sakh (V1), Campena (V2), Kharamana (V3), and Binicol (V5)] and one local variety, KSK-282 (V5). The findings revealed significant differences in the germination attributes among the rice lines. V1 and V3 showed superior germination performance, while V5 consistently exhibited the lowest values. The one-way analysis of variance (ANOVA) showed significant differences (p ≤ 0.05) across multiple parameters, including the coefficient of velocity of germination (CVG), germination index, mean germination rate (MGR), and synchronization index. A significant …

Digital Image Processing In Determining The Degree Of Turnip Yellows Virus Infection In Oilseed Rape, Alina-Ștefania Stanciu, Teodor Rusu, Alin Cristian Teușdea, Olimpia Smaranda Mintaș, Mihai Marcel Cărbunar, Cristian Oneț

Turkish Journal of Agriculture and Forestry

In agricultural crops, leaves play crucial roles in plant physiology, providing information on both the quantity and quality of future production. They also serve as indicators for diagnosing foliar diseases. Detecting plant diseases through image reading and computer vision in a specific format allows for both quantitative and qualitative studies, which is a key step in diagnosing diseases using images of leaves exhibiting symptoms specific to a pathogen. In this study, we present an approach that integrates digital image processing to assess the extent of turnip yellows virus (genus Polerovirus, family Solemoviridae) infection in oilseed rape. This study focuses on …

Seasonal Dynamics Of Biomass Productivity, Combustion Quality, And Nutrient Translocation In Bulbous Canary Grass (Phalaris Aquatica L.) In A Semiarid Mediterranean Environment, Recep İrfan Nazli, Mi̇hrab Polat, Asi̇ye Sena Çavdar, Veyi̇s Tansi

Turkish Journal of Agriculture and Forestry

In order to achieve sustainable biomass production from perennial grasses, seasonal changes in biomass yield, combustion quality, and nutrient translocation need to be monitored in detail. In this study, the effects of six distinct disruptive harvests (November, January, March, May, July, and September) on the dry biomass yield, ash, moisture, and mineral contents, as well as the composition of the cell walls and the translocation of nutrients in bulbous canary grass, were investigated in a semiarid Mediterranean climate. The September harvest significantly reduced aboveground ash and mineral contents, while their contents in belowground biomass exhibited a significantly increasing trend from …

From Ancient Wisdom To Modern Medicine: The Cultural And Therapeutic Significance Of Ficus Carica L. (Moraceae) Extracts In Colorectal Cancer Treatment, Esra Hacimüftüoğlu, Azizeh Shadidizaji, Kağan Tolga Ci̇ni̇sli̇, Songül Karakaya

Turkish Journal of Agriculture and Forestry

Figs, mentioned in the Torah, the Bible, and the Quran, are of historical and cultural significance and have been used for medical treatment, prompting extensive research. Colorectal cancer (CRC), the second leading cause of cancer-related death worldwide, is expected to significantly increase in terms of mortality rates by 2035, with its progression closely linked to inflammatory processes. Cyclooxygenase 2 (COX-2), an enzyme encoded by the Ptgs2 gene, plays a key role in these processes by converting arachidonic acid into prostaglandin E2 (PGE2), a mediator of inflammation. In the present study, the potential of natural antiinflammatory compounds, specifically fumaric acid and …

Erratum To “Coriander (Coriandrum Sativum L.) Diversity Analysis: Insights From Germplasm Resources Leading To Crop Improvement”[Turkish Journal Of Agriculture And Forestry 48 (6) 2024 876-887], Amit Baran Sharangi, Solanki Bal, Anirban Maji, Tarun Kumar Upadhyay, Reem Binsuwaidan, Nawaf Alshammari, Nadiyah M. Alabdallah, Adesh Kolapkar, Mohd Saeed

Turkish Journal of Agriculture and Forestry

We hereby issue this erratum to address a concern regarding the affiliation section in the manuscript titled “Coriander (Coriandrum sativum L.) diversity analysis: insights from germplasm resources leading to crop improvement.” A correction is issued regarding the affiliation detail in the originally published manuscript. The affiliation number for the ninth author, Mohd SAEED, was listed as 7, whereas it should have been 6. To amend this, the affiliation section in the manuscript has been revised as follows:

Link to the original article: https://journals.tubitak.gov.tr/agriculture/vol48/iss6/6/

Adapting To Climate Change In Semiarid Regions Via Conservation Measures: Climate-Smart Crop Rotations Of Food Legumes In Cool Seasons, Velibor Spalevic, Mohsen Janmohammadi, Naser Sabaghnia, Shuraik Kader

Turkish Journal of Agriculture and Forestry

The high potential of evapotranspiration, unsuitable soil conditions, irregular distribution of rainfall, and ineffective techniques of soil moisture conservation are among the limitations of semiarid regions of Central and West Asia and North Africa (CWANA), and they have intensified during recent decades with climate change. CWANA’s agroecosystems are fragile and highly vulnerable to the consequences of global warming and aggravation of water scarcity, and need to redefine agroclimatic zoning. Winter cereals are the predominant crops in the region of CWANA. The highest amount of cultivation is allocated to societies with dominant rainfed agriculture, which is often not economically profitable. The …

Remote Sensing Estimation Of Forest Ecosystem Service Values, Yu Zhang, Zhichao Guo, Guoshuang Tian, Minghua Lei

Turkish Journal of Agriculture and Forestry

A precise estimation of the value of forest ecosystem services (FESVs) has become essential due to the growing significance of the influence of global climate change on them. The primary objective of this study was to enhance the precision of forest service value monitoring and evaluation, thereby providing a scientific foundation for the promotion of sustainable forest resource management and ecological civilization construction. The study presented a remote sensing estimation (RSE) technique that combined time series characterization (TSC) and double-logistic regression (DLR) in order to achieve this. Through the integration of TSC analysis and a substantial amount of remote sensing …

Postharvest Application Of 1-Methylcyclopropene And Modified Atmosphere Packaging Extends Shelf Life And Maintains Eating Quality Of Mango Fruit Cv. Sufaid Chaunsa, Hafiz Muhammad Usman, Sajid Hussain, Raheel Anwar, Ahmad Sattar Khan, Aman Ullah Malik, Muhammad Moaaz Ali, Khurram Ziaf, Arooba Abbas

Turkish Journal of Agriculture and Forestry

The mango (Mangifera indica L.) fruit is globally relished for its sweet taste, unique aromatic profile, and enriched nutritional value. However, its short postharvest life is a serious challenge for export to long-distance markets. 1-Methylcyclopropene (1-MCP) is an ethylene inhibitor known to delay ripening in many fruits, including mango. However, comparative research suggesting an appropriate method to treat mangoes with 1-MCP is limited. In this study, mango fruit cv. Sufaid Chaunsa were fumigated with 0.25 ppm 1-MCP or treated aqueously with 1.2 ppm 1-MCP for 5 min and then packed in modified atmosphere packaging (MAP). The control fruits were not …