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Articles 4711 - 4740 of 10521
Full-Text Articles in Entire DC Network
Effects Of Whole Blood Viscosity And Plasma Nox On Cardiac Function And Cerebral Blood Flow In Children With Chronic Kidney Disease, Necla Buyan, Meltem Akçaboy, Tayfun Göktaş, Serdar Kula, Bi̇jen Nazliel, Ni̇lgün Çakar, Nermi̇n Uncu, Bülent Çeli̇k, Deni̇z Erbaş
Effects Of Whole Blood Viscosity And Plasma Nox On Cardiac Function And Cerebral Blood Flow In Children With Chronic Kidney Disease, Necla Buyan, Meltem Akçaboy, Tayfun Göktaş, Serdar Kula, Bi̇jen Nazliel, Ni̇lgün Çakar, Nermi̇n Uncu, Bülent Çeli̇k, Deni̇z Erbaş
Turkish Journal of Medical Sciences
Background/aim: The aim of the study was to investigate the effects of whole blood viscosity and plasma nitric oxide on cerebral and cardiovascular risks associated with chronic kidney disease. Materials and methods: The study group consisted of 40 pediatric patients and 21 healthy control subjects. Hematologic and biochemical variables, viscosity and plasma nitric oxide levels, echocardiographic findings, and middle cerebral artery blood flow velocity were examined. Results: Viscosity values of patients were significantly lower than those of the control group. Lower values of hematocrit, total protein, and albumin and higher values of ferritin in all patient groups resulted in significantly …
Multiplex Pcr Detection Of Problematic Pathogens Of Clinically Heterogeneous Bacterial Vaginosis In Bulgarian Women, Konstantsa Tosheva-Daskalova, Tanya Vasileva Strateva, Ivan Gergov Mitov, Raina Tzvetanova Gergova
Multiplex Pcr Detection Of Problematic Pathogens Of Clinically Heterogeneous Bacterial Vaginosis In Bulgarian Women, Konstantsa Tosheva-Daskalova, Tanya Vasileva Strateva, Ivan Gergov Mitov, Raina Tzvetanova Gergova
Turkish Journal of Medical Sciences
Background/aim: This study aimed to investigate the correlation between the prevalence of problematic pathogens and the clinical status of women with bacterial vaginosis (BV). Materials and methods: Gardnerella vaginalis, Atopobium vaginae, and Mobiluncus spp. were detected using a multiplex PCR assay, and their role in the infection of Bulgarian women with clinically heterogeneous BV was evaluated. Results: The predominant BV-associated pathogen identified was G. vaginalis with an incidence of 98.39%, followed by A. vaginae (68.05%) and Mobiluncus spp. at 17.01%. The coexistence of A. vaginae and G. vaginalis was more common in women with discharge (in 72.04%) and in patients …
The Histopathological And Stereological Assessment Of The Effect Of Topically Administered Leptin On Cerebral Vasospasm In An Experimental Subarachnoid Hemorrhage Model, Cem Demi̇rel, Cengi̇z Çokluk, Abdurrahman Aksoy, Kerameddi̇n Aydin, Mehmet Emi̇n Önger, Eni̇s Kuruoğlu, Abdullah Hi̇lmi̇ Marangoz, Ömür Gülsüm Deni̇z, Süleyman Kaplan
The Histopathological And Stereological Assessment Of The Effect Of Topically Administered Leptin On Cerebral Vasospasm In An Experimental Subarachnoid Hemorrhage Model, Cem Demi̇rel, Cengi̇z Çokluk, Abdurrahman Aksoy, Kerameddi̇n Aydin, Mehmet Emi̇n Önger, Eni̇s Kuruoğlu, Abdullah Hi̇lmi̇ Marangoz, Ömür Gülsüm Deni̇z, Süleyman Kaplan
Turkish Journal of Medical Sciences
Background/aim: Cerebral vasospasm is a term that refers to prolonged, slowly progressing but reversible pathological narrowing of cerebral arteries occurring several days after subarachnoid hemorrhage (SAH), usually accompanied by a decrease in perfusion distal to the affected artery. Leptin is an endogenous polypeptide hormone that can be carried freely and bound to protein in the blood. Materials and methods: We investigated the superiority of topical application of leptin that may make a contribution to the development of new treatment modalities for unconscious patients in brain injury intensive care units and its preventive effect, which is considered to have multifactorial pathogenesis …
Tnf-Α And Tgf-Ss Level After Intraoperative Allogeneic Red Blood Cell Transfusion In Orthopedic Operation Patients, Hamed Hassani, Alireza Khoshdel, Seyed Reza Sharifzadeh, Mohammad Foad Heydari, Shaban Alizadeh, Ali Noroozi Aghideh
Tnf-Α And Tgf-Ss Level After Intraoperative Allogeneic Red Blood Cell Transfusion In Orthopedic Operation Patients, Hamed Hassani, Alireza Khoshdel, Seyed Reza Sharifzadeh, Mohammad Foad Heydari, Shaban Alizadeh, Ali Noroozi Aghideh
Turkish Journal of Medical Sciences
Background/aim: Blood transfusion is associated with immunosuppression, referred to as transfusion-related immunomodulation (TRIM). In this study, for the first time, changes in the concentration of TGF-ß and TNF-α were measured postoperatively in orthopedic patients with intraoperational allogeneic red blood cell transfusion. Considering the use of packed cell units with different ages, it is possible to suggest the more appropriate product for clinical applications.Materials and methods: Two groups of 35 orthopedic surgery patients (with or without transfusion as case and control groups, respectively) were involved. Serum levels of TNF-α and TGF-ß were measured by ELISA.Results: The data suggested significant differences in …
Isolation And Culture Of Adult Mouse Vestibular Nucleus Neurons, Aydin Hi̇m, Serap Altuntaş, Gürkan Öztürk, Ender Erdoğan, Nureddi̇n Cengi̇z
Isolation And Culture Of Adult Mouse Vestibular Nucleus Neurons, Aydin Hi̇m, Serap Altuntaş, Gürkan Öztürk, Ender Erdoğan, Nureddi̇n Cengi̇z
Turkish Journal of Medical Sciences
Background/aim: Isolated cell cultures are widely used to study neuronal properties due to their advantages. Although embryonic animals are preferred for culturing, their morphological or electrophysiological properties may not reflect adult neurons, which may be important in neurodegenerative diseases. This paper aims to develop a method for preparing isolated cell cultures of medial vestibular nucleus (MVN) from adult mice and describe its morphological and electrophysiological properties.Materials and methods: Vestibular nucleus neurons were mechanically and enzymatically isolated and cultured using a defined medium with known growth factors. Cell survival was measured with propidium iodide, and electrophysiological properties were investigated with current-clamp …
In Vitro Evaluation Of Plla/Pbs Sponges As A Promisingbiodegradable Scaffold For Neural Tissue Engineering, İrem Ayşe Kanneci̇ Altinişik, Fatma Neşe Kök, Deni̇z Yücel, Gamze Köse
In Vitro Evaluation Of Plla/Pbs Sponges As A Promisingbiodegradable Scaffold For Neural Tissue Engineering, İrem Ayşe Kanneci̇ Altinişik, Fatma Neşe Kök, Deni̇z Yücel, Gamze Köse
Turkish Journal of Biology
In tissue engineering, the use of poly-L-lactic acid (PLLA)/polybutylene succinate (PBS) blend for the construction of scaffold is very limited. Moreover, polymeric sponges fabricated from PLLA/PBS have not been studied for neural tissue engineering. In the present study, the potential of the utility of PLLA/PBS polymeric sponges seeded with Schwann cells was investigated. PLLA and PBS were blended in order to increase the processability and tune the crystallinity, porosity, and degradation rate of the resulted polymeric sponges. These sponges were then seeded with Schwann cells. Porosity analysis showed that there were no significant differences between different compositions of PLLA/PBS blends; …
Targeted Delivery Of Etoposide To Osteosarcoma Cells Using Poly(3-Hydroxybutyrate-Co-3-Hydroxyvalerate) (Phbv) Nanoparticles, Esma Alp, Tamer Çirak, Murat Demi̇rbi̇lek, Mustafa Türk, Eylem Güven
Targeted Delivery Of Etoposide To Osteosarcoma Cells Using Poly(3-Hydroxybutyrate-Co-3-Hydroxyvalerate) (Phbv) Nanoparticles, Esma Alp, Tamer Çirak, Murat Demi̇rbi̇lek, Mustafa Türk, Eylem Güven
Turkish Journal of Biology
Folic acid (FA)-functionalized poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nanoparticles were prepared to enhance the delivery efficiency of the anticancer drug etoposide for the clinical treatment of osteosarcoma. PHBV nanoparticles were synthesized by emulsification/solvent evaporation technique and obtained in the size range of 200-250 nm and zeta potential range of -21 and -27 mV. Encapsulation efficiency and in vitro drug release were studied. The cytotoxic, apoptotic, and necrotic effects of PHBV nanoparticles were also investigated using Saos-2 osteosarcoma cells. The results obtained in this study demonstrate that etoposide-loaded and FA-functionalized PHBV nanoparticles can be successfully used for targeted treatment of osteosarcoma.
N-Acetylglucoseamine Modified Alginate Sponges As Scaffolds For Skin Tissue Engineering, Murat Demi̇rbi̇lek, Neli̇sa Türkoğlu, Selçuk Aktürk
N-Acetylglucoseamine Modified Alginate Sponges As Scaffolds For Skin Tissue Engineering, Murat Demi̇rbi̇lek, Neli̇sa Türkoğlu, Selçuk Aktürk
Turkish Journal of Biology
In the treatment of dermal wounds, wound-dressing materials prepared from natural mucopolysaccharides are widely used because of their advantages such as nonirritation, nontoxicity, and ease in topical application. In the present study, alginate hydrogels modified with N-acetyl glucose amine (NAG) were prepared as wound-dressing material. Physical, chemical, thermal, and mechanical properties of the hydrogels were studied. Cytotoxicity of the hydrogels on endothelial (HUVEC) and keratinocyte (HaCaT) cells were examined. Anti- and proinflammatory cytokine levels of human monocyte-macrophage cells (THP-1) stimulated with hydrogels were determined. According to the results, increasing the NAG concentration led to an increase in the swelling and …
Accumulation Of Phenolics In Natural And Micropropagated Plantlets Of Thymus Pseudopulegioides Klokov & Des.-Shost. With Their Antioxidant Potentials, Mustafa Günaydin, Abdul Hafeez Laghari, Ersan Bektaş, Münevver Sökmen, Atalay Sökmen
Accumulation Of Phenolics In Natural And Micropropagated Plantlets Of Thymus Pseudopulegioides Klokov & Des.-Shost. With Their Antioxidant Potentials, Mustafa Günaydin, Abdul Hafeez Laghari, Ersan Bektaş, Münevver Sökmen, Atalay Sökmen
Turkish Journal of Biology
Thymus pseudopulegioides plantlets were propagated in vitro via direct organogenesis by using Murashige and Skoog (MS) media containing kinetin, thidiazuron, and 6-benzyladenine (BA) individually. Methanol extracts obtained both from plantlets and wild plants were analyzed for their total phenolics and flavonoid contents, then quantified by HPLC. The highest total phenolic (8.83 mg/g as gallic acid equivalent) and total flavonoid (0.92 mg/mL as rutin equivalent) values were from the MS media supplemented with 1.0 mg/L kinetin and 0.5 mg/L BA, respectively. The plantlets grown in those media also showed remarkable antioxidant activities with an IC50 value of 4.77 \mug/mL in DPPH …
In Vitro Transcription And Validation Of Human Pancreatic Transcription Factors' Mrnas, Ersi̇n Akinci, Mehmet Yildiz, Peli̇n Ünal, Gamze Badakul
In Vitro Transcription And Validation Of Human Pancreatic Transcription Factors' Mrnas, Ersi̇n Akinci, Mehmet Yildiz, Peli̇n Ünal, Gamze Badakul
Turkish Journal of Biology
Direct reprogramming of pancreatic beta cells is of great interest due to its possible use in diabetes treatment. Various studies have demonstrated direct reprogramming of insulin expressing beta cells from other somatic cells by overexpressing important pancreatic transcription factors through viral vectors. However, concerns about using viral vectors in the clinic have prompted scientists to find new approaches for gene delivery and expression. In vitro synthesis and transfection of mRNAs is a safer strategy for ectopic gene expression. In this study we aimed to design and in vitro synthesize human pancreatic transcription factors' mRNAs: Pdx1, Ngn3, and MafA. We also …
Effects Of Celecoxib And L-Name On Apoptosis And Cell Cycle Ofmcf-7 Cd44+/Cd24-/Low Subpopulation, Maryam Majdzadeh, Shima Aliebrahimi, Melody Vatankhah, Seyed Nasser Ostad
Effects Of Celecoxib And L-Name On Apoptosis And Cell Cycle Ofmcf-7 Cd44+/Cd24-/Low Subpopulation, Maryam Majdzadeh, Shima Aliebrahimi, Melody Vatankhah, Seyed Nasser Ostad
Turkish Journal of Biology
Recent studies have reported that cancer stem cells (CSCs) play a pivotal role in treatment failure, causing cancer recurrence. Here, we investigated the effects of L-NAME (an iNOS inhibitor) and celecoxib (a selective COX-2 inhibitor) on CSC-like cells (CSC-LCs) and their parental cells. Breast CSC-LCs derived from the MCF-7 cell line were sorted and characterized with the CD44+/CD24-/low phenotype. After isolation, the percentage of the subpopulation expressing CD44+/CD24-/low biomarkers increased considerably from 0.96% to 28.6%. Use of L-NAME and celecoxib showed antiproliferative activity towards both MCF-7 and CSC-LCs. Although celecoxib enhanced apoptotic cell death, the CSC-LC population was more resistant …
Analysis Of Ca125 Antigen In Normal Human Seminal Plasma Highlightsthe Molecular Heterogeneity Of Underlying Glycosylated Species, Ninoslav Mitic, Bojana Milutinovic, Miroslava Jankovic
Analysis Of Ca125 Antigen In Normal Human Seminal Plasma Highlightsthe Molecular Heterogeneity Of Underlying Glycosylated Species, Ninoslav Mitic, Bojana Milutinovic, Miroslava Jankovic
Turkish Journal of Biology
Cancer antigen CA125 represents an extracellular part of transmembrane mucin MUC16 and may be expressed in tissues of the male reproductive tract. It reaches human seminal plasma (hSP) after undergoing the main processes involved in protein speciation: synthesis, posttranslational modifications, compartmentalization, and auto/proteolytic degradation. This study was aimed at profiling CA125-immunoreactive species in hSP from healthy subjects as a specific and unexplored source of this tumor-associated antigen expressed under normal physiological conditions. High molecular mass components from total hSP and corresponding acid-soluble hSP preparations were analyzed. The results indicated that antibodies recognizing two distinct immunogenic areas of CA125 antigen exhibited …
Characterization Of The Promoter Region Of The Glycerol-3-Phosphate-O-Acyltransferase Gene In Lilium Pensylvanicum, Lijing Chen, Li Zhang, Weikang Qi, Muhammad Irfan, Jing-Wei Lin, Hui Ma, Zhi-Fu Guo, Ming Zhong, Tian-Lai Li
Characterization Of The Promoter Region Of The Glycerol-3-Phosphate-O-Acyltransferase Gene In Lilium Pensylvanicum, Lijing Chen, Li Zhang, Weikang Qi, Muhammad Irfan, Jing-Wei Lin, Hui Ma, Zhi-Fu Guo, Ming Zhong, Tian-Lai Li
Turkish Journal of Biology
Cold environmental conditions influence the growth and development of plants, causing crop reduction or even plant death. Under stress conditions, cold-inducible promoters regulate cold-related gene expression as a molecular switch. Recent studies have shown that the chloroplast-expressed GPAT gene plays an important role in determining cold sensitivity. However, the mechanism of the transcriptional regulation of GPAT is ambiguous. The 5'-flanking region of GPAT with length of 1494 bp was successfully obtained by chromosome walking from Lilium pensylvanicum. The cis-elements of GPAT promoters were predicted and analyzed by a plant cis-acting regulatory DNA element database. There exist core promoter regions including …
Ampicillin Activates Mpk1 Phosphorylation In Saccharomyces Cerevisiaeand Erk1/2 Phosphorylation In Hepg2 Cells, Yukyong Shin, Kiyoung Kim
Ampicillin Activates Mpk1 Phosphorylation In Saccharomyces Cerevisiaeand Erk1/2 Phosphorylation In Hepg2 Cells, Yukyong Shin, Kiyoung Kim
Turkish Journal of Biology
Ampicillin has been widely used to treat bacterial infections. When we used ampicillin to eliminate bacterial contamination in yeast cultures, we observed induction of phosphorylation of MAP kinase 1 (Mpk1), a previously unknown function of ampicillin. We therefore investigated whether ampicillin activates the signal transduction pathway. Phosphorylation of Saccharomyces cerevisiae Mpk1 was induced by ampicillin in a dose- and time-dependent manner through the PKC1-CWI pathway. Mpk1 phosphorylation was maximal after treatment with 3 mM ampicillin for 90 min. Despite activation of Mpk1 phosphorylation, ampicillin did not influence yeast cell growth. Ampicillin reduced miconazole antifungal activity; miconazole had a minimum inhibitory …
A R2r3 Myb Transcription Factor From Ash Positively Regulates Salt Response In Tobacco, Tian Li, Jingkuan Sun, Yuping Bi
A R2r3 Myb Transcription Factor From Ash Positively Regulates Salt Response In Tobacco, Tian Li, Jingkuan Sun, Yuping Bi
Turkish Journal of Biology
The roles of MYB transcription factors are diverse and important in regulating environmental stress in plants. In this study, the R2R3 MYB gene FvMYB2 (GenBank Accession No. KY767843) and its promoter region were cloned from ash (Fraxinus velutina Torr.). Phylogenetic analysis revealed that FvMYB2 was clustered with VvMYB60 from Vitis vinifera. PlantCARE software analysis showed that its promoter contained different cis elements involved in diverse abiotic stresses. The expression patterns of FvMYB2 were investigated under different abiotic stress conditions. FvMYB2 subcellular localization was mainly localized in the nucleus. Overexpression of FvMYB2 in tobacco showed enhanced salt resistance and upregulation of …
Optimization Of Monoclonal Antibody Expression In Chocells By Employing Epigenetic Gene Regulation Tools, Fatemeh Nematpour, Fereidoun Mahboudi, Vahid Khalaj, Behrouz Vaziri, Samira Ahmadi, Maryam Ahmadi, Saedeh Ebadat, Fatemeh Davami
Optimization Of Monoclonal Antibody Expression In Chocells By Employing Epigenetic Gene Regulation Tools, Fatemeh Nematpour, Fereidoun Mahboudi, Vahid Khalaj, Behrouz Vaziri, Samira Ahmadi, Maryam Ahmadi, Saedeh Ebadat, Fatemeh Davami
Turkish Journal of Biology
Monoclonal antibodies (mAbs) are crucial in pharmaceutical biotechnology. Mammalian cell lines are the most preferred for their production. One of the significant challenging issues of mammalian expression systems is epigenetic gene silencing. Employing epigenetic gene regulation tools can increase the productivity of the mammalian cell lines. Sodium butyrate (NaBut) and valproic acid (VPA) regulate gene expression by inhibiting histone deacetylase. A ubiquitous chromatin-opening element (UCOE) can improve expression by reducing DNA methylation. Here, the separate and combined effects of NaBut and VPA histone deacetylase inhibitors (iHDACs) and UCOE on mAb synthesis were studied. Stable cell lines were generated by non-UCOE …
Aggregation Of A Parthenogenetic Diploid Embryo And A Male Embryo Improves The Blastocyst Development And Parthenogenetic Embryonic Stem Cell Derivation, Xiaoyan Qiu, Nan Li, Xiong Xiao, Yuemin Li
Aggregation Of A Parthenogenetic Diploid Embryo And A Male Embryo Improves The Blastocyst Development And Parthenogenetic Embryonic Stem Cell Derivation, Xiaoyan Qiu, Nan Li, Xiong Xiao, Yuemin Li
Turkish Journal of Biology
Parthenogenetically derived mammalian embryos, with no paternal genome, are not viable and die, largely from defective placental growth attributed to a lack of paternal effect, resulting in the low blastulation rate and low derivation efficiency of parthenogenetic embryonic stem cells (pESCs). Therefore, the present study, by the optimization of parthenogenetic embryo production and the aggregation of the parthenogenetic diploid embryo and the identified male embryo, aims to investigate a method to improve the development of parthenogenetic embryo and pESC derivation in mice. Using different chemical combinations for the optimization, we found that the heterozygous diploid type had a significantly higher …
Comparative Phytotoxicity Of Undoped And Er-Doped Zno Nanoparticles Onlemna Minor L.: Changes In Plant Physiological Responses, Samaneh Torbati, Alireza Khataee, Shabnam Saadi
Comparative Phytotoxicity Of Undoped And Er-Doped Zno Nanoparticles Onlemna Minor L.: Changes In Plant Physiological Responses, Samaneh Torbati, Alireza Khataee, Shabnam Saadi
Turkish Journal of Biology
The present study is the first research on the phytotoxicological effects of Er-doped zinc oxide nanoparticles (NPs) on duckweed (Lemna minor L.), as a model aquatic floating macrophyte. It concentrated on the comparison of the physiological effects of different concentrations of undoped and Er-doped zinc oxide NPs on L. minor. Pure and Er-doped zinc oxide samples were synthesized through a sonochemical method and their morphology and chemical composition were studied by scanning electron microscopy and energy dispersive X-ray analysis. Plant growth, photosynthetic pigment contents, and antioxidant enzyme activities were investigated as indices to assess the effects and toxicity of the …
Regulation Of Functional Activities And Essential Oil Production In Vetiveria Zizanioides L. Nash After γ-Irradiated Sodium Alginate Elicitation, Asfia Shabbir, Mohammad Masroor Akhtar Khan, Yawar Sadiq, Hassan Jaleel, Bilal Ahmad, Moin Uddin
Regulation Of Functional Activities And Essential Oil Production In Vetiveria Zizanioides L. Nash After γ-Irradiated Sodium Alginate Elicitation, Asfia Shabbir, Mohammad Masroor Akhtar Khan, Yawar Sadiq, Hassan Jaleel, Bilal Ahmad, Moin Uddin
Turkish Journal of Biology
Upon irradiation, marine polysaccharides undergo depolymerization, leading to formation of oligosaccharides that elicit various biological activities in plants. Taking a step further on the previously established growth-promoting activity of irradiated sodium alginate (ISA), structural rearrangements in ISA were analyzed using complementary techniques to develop an understanding of the structure-property relationship. The essential oil (EO) of vetiver (Vetiveria zizanioides L. Nash) immensely benefits the perfumery industry, proving itself as an economically important crop. A pot experiment was designed to test the effect of water-soluble ISA fractions on the growth, physiology, and EO production of vetiver. The structural characterization of radiation-induced sodium …
Changes In Apoptosis-Related Gene Expression Profiles In Cancer Cell Lines Exposed To Usnic Acid Lichen Secondary Metabolite, Adnan Berk Di̇nçsoy, Demet Cansaran Duman
Changes In Apoptosis-Related Gene Expression Profiles In Cancer Cell Lines Exposed To Usnic Acid Lichen Secondary Metabolite, Adnan Berk Di̇nçsoy, Demet Cansaran Duman
Turkish Journal of Biology
The presence of uninhibited side effects of cancer drugs often used in cancer treatment has stimulated the search for alternative therapeutic approaches. Therefore, anticarcinogenic effects of synthetic, herbal, and fungal drugs have been investigated for the treatment of various cancer types in recent studies. Lichens, symbiotic organisms of fungi and algae, synthesize metabolites with significant biological activities. The aim of the current study was to screen the anticancer potential of usnic acid on various types of nonmalignant cell lines (Vero, L929) and cancer cell lines (CaCo2, RD, Hep2C, HepG2, Wehi). The growth inhibitory effect of usnic acid was determined by …
Characterization Of Proteinase Inhibitor-Ii Gene Under Osrglp2promoter For Salt Stress In Transgenic Nicotiana Benthamiana, Shazia Rehman, Bodil Jørgensen, Soren Rasmussen, Tariq Mahmood
Characterization Of Proteinase Inhibitor-Ii Gene Under Osrglp2promoter For Salt Stress In Transgenic Nicotiana Benthamiana, Shazia Rehman, Bodil Jørgensen, Soren Rasmussen, Tariq Mahmood
Turkish Journal of Biology
Proteinase inhibitors in plants are mainly involved in conferring resistance against herbivores and pathogens. However, their role in abiotic stress resistance has also been reported. Therefore, transgenic tobacco plants were generated constitutively expressing the tomato proteinase inhibitor-II (PI-II) under control of the rice root germin-like protein 2 (OsRGLP2) promoter using Agrobacterium tumefaciens. T1 transgenic seedlings were subjected to stress treatments with different concentrations of sodium chloride (NaCl) (100 mM, 200 mM, 300 mM). Quantitative real-time PCR revealed considerable levels of expression and upregulation of PI-II in transgenic lines under salt stress. Considering the phenotypic effects, the transgenic seedlings showed better …
Cell-Compatible Phb/Silk Fibroin Composite Nanofibermat For Tissue Engineering Applications, Zeynep Karahali̇loğlu
Cell-Compatible Phb/Silk Fibroin Composite Nanofibermat For Tissue Engineering Applications, Zeynep Karahali̇loğlu
Turkish Journal of Biology
Cell compatibility is one of the prominent requirements for the fabrication of tissue engineering materials. Silk fibroin (SF) is an excellent material for biomedical applications and shows desirable properties such as good compatibility, minimal tissue reaction, and tailorable degradability. Poly[(R)-3-hydroxybutyrate] (PHB) has a high percentage of crystallinity and a high melting temperature. In this study, PHB and SF were blended together to improve the mechanical properties of the SF fibrous structure and the crystallinity of PHB. Furthermore, the cell-biomaterial interaction on the PHB/SF composite scaffold was expected to be enhanced via the SF. For this purpose, PHB/SF composite scaffolds were …
Role Of Snf5 In Rheumatoid Arthritis By Upregulation Ofp16 And Inactivation Of Jnk Pathway, Shupeng Wu, Jing Wang, Fang Li
Role Of Snf5 In Rheumatoid Arthritis By Upregulation Ofp16 And Inactivation Of Jnk Pathway, Shupeng Wu, Jing Wang, Fang Li
Turkish Journal of Biology
This study aims to explore the role and the possible underlying molecular mechanism of SNF5 in the pathogenesis of rheumatoid arthritis (RA). MH7A cells were respectively transfected with pc-SNF5 (pcDNA3.1 containing the Brg1 coding sequence), short hairpin RNA against SNF5 (shSNF5), and their negative controls (pcDNA3.1 and shNC). The alterations of SNF5 expression were assessed by qRT-PCR and western blot analysis. MTT assay, flow cytometry, and western blot analysis were performed to evaluate proliferation, apoptosis, and expression levels of p16 and JNK pathway associated proteins, respectively. Finally, the effect of SNF5 was verified in fibroblast-like synoviocytes (FLSs) obtained from a …
High-Fat Diet And Glucose And Albumin Circadian Rhythms' Chronodisruption In Rats, Rafael Bravo, Americo Chini, Lierni Ugartemendia, Lourdes Franco, Mónica Mesa, Ana B. Rodriguez, Javier Cubero, Carmen Barriga
High-Fat Diet And Glucose And Albumin Circadian Rhythms' Chronodisruption In Rats, Rafael Bravo, Americo Chini, Lierni Ugartemendia, Lourdes Franco, Mónica Mesa, Ana B. Rodriguez, Javier Cubero, Carmen Barriga
Turkish Journal of Biology
Obesity is one of the most widespread nutritional diseases in developed societies and it is considered a cardiovascular disease risk factor. The aim of the present work was to evaluate how a high-fat diet may influence the chronobiology of glucose and albumin circadian rhythms. Eighty-four male common Wistar rats were separated into two groups: a control group (n = 42) and a group fed a high-fat diet (n = 42); both for the control and the obesity-induced group we stablished 7 subgroups (6 rats per subgroup) to take blood samples at 0000, 0200, 0400, 1000, 1400, 1800, and 2200 hours. …
The Comparison Of Antioxidant Capacity And Cytotoxic, Anticarcinogenic,And Genotoxic Effects Of Fe@Au Nanosphere Magnetic Nanoparticles, Hande Yegenoglu, Belma Aslim, Burcu Guven, Adem Zengin, İsmai̇l Hakki Boyaci, Zeki̇ye Suludere, Uğur Tamer
The Comparison Of Antioxidant Capacity And Cytotoxic, Anticarcinogenic,And Genotoxic Effects Of Fe@Au Nanosphere Magnetic Nanoparticles, Hande Yegenoglu, Belma Aslim, Burcu Guven, Adem Zengin, İsmai̇l Hakki Boyaci, Zeki̇ye Suludere, Uğur Tamer
Turkish Journal of Biology
Magnetic gold nanoparticles are used in various biomedical, biochemistry, and biotechnology applications due to their controllable size distribution, long-term stability, reduced toxicity, and biocompatibility. Different coating materials, such as proteins, carbohydrates, lipids, and polyphenols, are applied to enhance the biocompatibility of nanoparticles. In this study, the effects of surface coatings of core-shell structured Fe@Au nanosphere magnetic nanoparticles with regard to antioxidant capacity and cytotoxic, anticarcinogenic, and genotoxic properties were investigated. The obtained results demonstrated that avidin-coated Fe@Au nanospheres had higher antioxidant capacities than uncoated nanospheres. Neither avidin-coated nor uncoated nanoparticles had a cytotoxic effect on normal cells (human gingival fibroblast …
Amperometric Biosensor For Detection Of Triglyceride Tributyrinbased On Zero Point Charge Of Activated Carbon, Mohsen Rezvani, Ghasem Najafpour, Maedeh Mohammadi, Hossein Zare
Amperometric Biosensor For Detection Of Triglyceride Tributyrinbased On Zero Point Charge Of Activated Carbon, Mohsen Rezvani, Ghasem Najafpour, Maedeh Mohammadi, Hossein Zare
Turkish Journal of Biology
A simple and robust single enzyme biosensor was fabricated for triglyceride (TG) detection. Graphite rods were modified with activated carbon (AC) and used as support for lipase immobilization. Chitosan (CHIT) was eventually used to create film on the bioelectrode and retain the immobilized enzyme. To extend the linear range of TG detection, AC was functionalized with carboxyl and then amine groups (AAC) to enhance the isoelectric point of AC. The constructed graphite/AAC/lipase /CHIT bioelectrode was characterized using cyclic voltammetry and field emission scanning electron microscopy (FE-SEM). The accuracy of the developed biosensor was assessed through determination of different concentrations of …
Exogenously Sourced γ-Irradiated Chitosan-Mediated Regulation Of Growth, Physiology, Quality Attributes And Yield In Mentha Piperita L., Bilal Ahmad, Mohammad Masroor Akhtar Khan, Hassan Jaleel, Yawar Sadiq, Asfia Shabbir, Moin Uddin
Exogenously Sourced γ-Irradiated Chitosan-Mediated Regulation Of Growth, Physiology, Quality Attributes And Yield In Mentha Piperita L., Bilal Ahmad, Mohammad Masroor Akhtar Khan, Hassan Jaleel, Yawar Sadiq, Asfia Shabbir, Moin Uddin
Turkish Journal of Biology
In view of the increasing demand for essential oils from medicinal and aromatic plants, the application of irradiated polysaccharides like chitosan is a promising option. A pot experiment was performed to study the influence of various concentrations of irradiated chitosan (ICN) on growth, physiological, and yield parameters of Mentha piperita L., a plant with considerable pharmaceutical importance. The treatments were foliar spray of deionized water only (control), foliar spray of unirradiated chitosan (40 mg L-1), and ICN at 40, 80, 120, and 160 mg L-1. Among these, 80 mg L-1 ICN (treatment 4) proved to be the best for most …
Microrna-34a Regulates Brain-Derived Neurotrophic Factorin An Intracerebral Hemorrhage Model, Jin Hee Kim, Jae-Sun Choi
Microrna-34a Regulates Brain-Derived Neurotrophic Factorin An Intracerebral Hemorrhage Model, Jin Hee Kim, Jae-Sun Choi
Turkish Journal of Biology
Intracerebral hemorrhages (ICHs) are devastating neurological events frequently resulting in a serious negative prognosis. The exact physiological and disease processes involved in ICHs are complex, but are thought to involve microRNAs (miRNAs), 22 nucleotide small noncoding RNAs that control a variety of normal physiological and disease processes. In this study, we show that a miRNA, miR-34a, regulates BDNF in a model of ICH injury. In particular, we assessed the impact of AM34a, an inhibitor of miR-34a, on the toxicity of thrombin-induced apoptosis and on BDNF-mediated signaling. We investigated the increased expression of miR-34a after an ICH-induced thrombin toxicity injury using …
The Role Of Predicted Spermidine Family Transporters In Stressresponse And Cell Cycle In Schizosaccharomyces Pombe, Aslihan Gevrekci̇
The Role Of Predicted Spermidine Family Transporters In Stressresponse And Cell Cycle In Schizosaccharomyces Pombe, Aslihan Gevrekci̇
Turkish Journal of Biology
Fission yeast Schizosaccharomyces pombe has a variety of stress-signaling proteins that protect cells against environmental or intracellular stress. These proteins help the cells to respond to stress conditions and regulate intracellular functions such as cell division or gene expression. Polyamines (spermidine, spermine, and putrescine) are known to be important in the regulation of stress response and cell division. In this study, we tried to experimentally characterize novel S. pombe genes that are involved in the polyamine pathway and understand their potential roles. Sequence analysis revealed four genes that code for (predicted) spermidine family transporters in S. pombe. In an attempt …
Histomorphometric Evaluation Of The Testicular Parenchyma Of Rats Submittedto Protein Restriction During Intrauterine And Postnatal Life, Jessica Oliveira, Alluanan Silva, Sandra Souza, Rosana Morais, Elizabeth Neves Melo, Frederico Maia, Valdemiro Silva Junior
Histomorphometric Evaluation Of The Testicular Parenchyma Of Rats Submittedto Protein Restriction During Intrauterine And Postnatal Life, Jessica Oliveira, Alluanan Silva, Sandra Souza, Rosana Morais, Elizabeth Neves Melo, Frederico Maia, Valdemiro Silva Junior
Turkish Journal of Biology
The critical period of development is highly susceptible to disorders. Environmental contaminants, stress, and poor nutrition may permanently affect structurally and functionally an organism during adulthood. Protein restriction in intrauterine and neonatal periods may impair testicular cells and reduce steroidogenic activity. The current study investigated the effect of low protein diet during intrauterine and postnatal life on testicular function in immature and adult rats. Pregnant Wistar rats were fed either a normal protein diet or a low protein diet during pregnancy until birth and during lactation until weaning. The results showed that undernutrition during intrauterine life reduced epithelium height, tubular …