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Preparation Of Neuraminidase-Specific Antiserum From The H9n2 Subtype Of Avian Influenza Virus, Ali Nazari Shirvan, Mehran Moradi, Mehdi Aminian, Rasool Madani
Preparation Of Neuraminidase-Specific Antiserum From The H9n2 Subtype Of Avian Influenza Virus, Ali Nazari Shirvan, Mehran Moradi, Mehdi Aminian, Rasool Madani
Turkish Journal of Veterinary & Animal Sciences
Avian influenza virus stocks (A/chicken/Iran/259/1998/(H9N2)) were propagated in the allantoic cavities of 10-day-old embryonated chicken eggs. The harvested suspension was concentrated by polyethylene glycol 6000. Concentrated samples were layered onto sucrose gradient (30%-60%). Both hemagglutinin and neuraminidase were solubilized from purified viruses with Triton X-100 across 30% sucrose gradient. NA was isolated from HA and other viral proteins by affinity chromatography on N-(p-aminophenyl)oxamic acid. Fractions that had high NA activity and did not show HA activity were pooled and analyzed by neuraminidase inhibition and SDS-PAGE. No viral protein bands were detected, except for a single band in the position of …
Verotoxin Production In Strains Of Escherichia Coli Isolated From Cattle And Sheep, And Their Resistance To Antibiotics, Altan Aksoy, Murat Yildirim, Bi̇rgül Kaçmaz, Teoman Zafer Apan, J. Sedef Göçmen
Verotoxin Production In Strains Of Escherichia Coli Isolated From Cattle And Sheep, And Their Resistance To Antibiotics, Altan Aksoy, Murat Yildirim, Bi̇rgül Kaçmaz, Teoman Zafer Apan, J. Sedef Göçmen
Turkish Journal of Veterinary & Animal Sciences
In this study, 130 Escherichia coli strains isolated from 306 cattle and sheep fecal samples were studied for their resistance to 10 antibiotics, verotoxin production, and hemolyzation. The antibiotic resistance rates of the E. coli strains were as follows: tetracycline, 51.6%; streptomycin, 24.2%; ampicillin, 13.1%; amoxicillin/clavulanic acid, 5.2%; gentamycin, 4.6%; ciprofloxacin, 4.6%; trimethoprim-sulfamethoxazole, 4.3%; cefotaxime, 0.7%. None of the strains were resistant to cefepime or ceftazidime. Of all the antibiotics tested, only resistance to streptomycin was higher in the strains isolated from cattle than in the sheep strains (P = 0.043). The evaluation of the cattle strains, based on gender …
Protein Profile And Plasmid Content Of Lactococcus Lactis Subsp. Lactis Ll52 And Lactococcus Lactis Subsp. Cremoris Lc79 Strains Under Several Stress Conditions, Rahmi̇ Lale, Çağla Tükel, Mustafa Akçeli̇k
Protein Profile And Plasmid Content Of Lactococcus Lactis Subsp. Lactis Ll52 And Lactococcus Lactis Subsp. Cremoris Lc79 Strains Under Several Stress Conditions, Rahmi̇ Lale, Çağla Tükel, Mustafa Akçeli̇k
Turkish Journal of Veterinary & Animal Sciences
Differences in the protein and plasmid content of 2 Lactococcus lactis strains, L. lactis subsp. lactis LL52 and L. lactis subsp. cremoris LC79, under the stresses of high and low temperature, osmotic shock, and low pH were determined. We identified 3 new proteins with molecular masses of 16.0, 29.4, and 45.0 kDa as high temperature stress response specific in strain LL52. High temperature stress did not cause any changes in the protein content of strain LC79. Proteins that were specific for salt stress and low pH stress responses ranged between 16.0 and 40.5 kDa, and 24.8 and 107.5 kDa, respectively, …
Detection Of Antibodies Produced In Quails (Coturnix Coturnix Japonica) Against Mycoplasma Gallisepticum With Different Serological Tests, Süheyla Türkyilmaz, Fethi̇ye Çöven, Seza Eski̇i̇zmi̇rli̇ler
Detection Of Antibodies Produced In Quails (Coturnix Coturnix Japonica) Against Mycoplasma Gallisepticum With Different Serological Tests, Süheyla Türkyilmaz, Fethi̇ye Çöven, Seza Eski̇i̇zmi̇rli̇ler
Turkish Journal of Veterinary & Animal Sciences
The objectives of this study were to isolate and identify Mycoplasma gallisepticum in quails with clinical and pathological signs of chronic respiratory disease (CRD), and to compare the sensitivity and specificity of commonly used serological tests that are used for the detection of M. gallisepticum antibodies. The study included 20 Japanese quails 10-weeks old with respiratory distress, nasal discharge, mortality and swollen infraorbital sinuses. There was no bacteriological agent isolated. All of the 20 sera samples obtained from the quails were evaluated with AGID, RSA, HI, and ELISA; 2 (10%), 5 (25%), 10 (50%), and 17 (85%) sera samples were …
The Seroprevalence Of Listeria Monocytogenes In Sport Horses Bred In Ankara Province, H. Zeynep Güçlü, K. Zafer Karaer, Cahi̇t Babür, Selçuk Kiliç
The Seroprevalence Of Listeria Monocytogenes In Sport Horses Bred In Ankara Province, H. Zeynep Güçlü, K. Zafer Karaer, Cahi̇t Babür, Selçuk Kiliç
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to determine the seroprevalence of Listeria monocytogenes in horses bred for sport in the province of Ankara. Sera collected from 100 clinically healthy horses were tested for the presence of L. monocytogenes antibodies using the Osebold absorption test. Agglutination titers >= 1:100 were accepted as positive results and a seropositivity rate was determined. Of the 100 serum samples, 62 were seropositive for L. monocytogenes antibodies detected at dilutions of 1:100 (n = 29, 46.7%), 1:200 (n = 31, 50%), and 1:400 (n = 2, 3.2%).
Anatomical And Histological Studies Of Accessory Adrenal Nodules In Caspian Miniature Horses, Javad Ahmadpanahi
Anatomical And Histological Studies Of Accessory Adrenal Nodules In Caspian Miniature Horses, Javad Ahmadpanahi
Turkish Journal of Veterinary & Animal Sciences
Microscopic and macroscopic structures of accessory adrenal nodules in Caspian miniature horses (5 males and 5 females) were studied. The accessory adrenal nodules were round or oval and yellow to red. Their maximum diameters varied from 0.1 to 0.5 cm. These nodules were found near the kidneys and adrenal glands, but they were in close contact with the adrenal gland. The accessory adrenal nodules were similar to the adrenal cortex microscopically, and from the outer layer to the inner layer they contained glomerulosa, intermedia, and fasciculata. The adrenal nodules were surrounded by a capsule of collagenous dense connective tissue as …
Cover And Contents
Turkish Journal of Veterinary & Animal Sciences
No abstract provided.
Effect Of Feeding Supplemental Tallow On The Performance Of Lactating Nili-Ravi Buffaloes, Haq Nawaz, Muhammad Yaqoob, Muhammad Abdullah
Effect Of Feeding Supplemental Tallow On The Performance Of Lactating Nili-Ravi Buffaloes, Haq Nawaz, Muhammad Yaqoob, Muhammad Abdullah
Turkish Journal of Veterinary & Animal Sciences
In order to determine the feeding value of tallow for lactating Nili-Ravi buffaloes, 4 buffaloes were fed 0%, 2%, 4%, or 6% tallow in a 4 X 4 Latin square experiment. The intakes of dry matter (DM), organic matter (OM), crude protein (CP), acid detergent fiber (ADF), and neutral detergent fiber (NDF) decreased (P < 0.01), but the intakes of ether extract (EE) (P < 0.01) and digestible energy (DE) (P < 0.05) increased with increasing levels of tallow in the diets. Intake of net energy for lactation (NE_L) did not differ (P < 0.01) with varying levels of supplemental tallow. Daily milk yield increased (P < 0.01) from 11.00 to 13.2 kg/day. Production of 4% fat corrected milk (FCM), solid corrected milk (SCM), and energy corrected milk (ECM) increased quadratically (P < 0.05) with the increasing level of tallow in the diets. Milk fat content and total milk fat increased linearly (P < 0.01) and quadratically (P < 0.05), respectively, as the level of tallow in the diet increased. There was no difference in protein and lactose content due to feeding tallow. Total solid content increased quadratically (P < 0.05) from 16.45% to 17.67% but solid not fat (SNF) percentages did not vary with varying levels of tallow. The gross energy (GE) of the milk increased from 1096 to 1160 Kcal/kg (P < 0.01) with increasing levels of tallow in the diets. Energetic efficiency of milk production improved in a quadratic (P < 0.05) manner with 2%, 4%, and 6% tallow, with the highest being 47.6% with 4% supplemental tallow. The proportion of C_{8:0} to C_{16:1} fatty acids (FA) decreased from 53.66% to 35.52%, whereas the concentration of C_{18:0} to C_{20:0} increased (44.93% to 62.84%) in the milk fat of buffaloes fed diets containing different levels of tallow. Gains in body weight (BW) were higher (P < 0.05) in buffaloes fed diets containing supplemental tallow as compared to those fed the control diet. This study suggests that tallow up to 6% of diet DM is a suitable fat supplement as an economical energy source for lactating buffaloes.
Allelic Frequency Of Kappa-Casein And Beta-Lactoglobulin In Indian Crossbred (Bos Taurus X Bos Indicus) Dairy Bulls, Rajesh K. Patel, Jenabhai B. Chauhan, Krishna M. Singh, Kalpesh J. Soni
Allelic Frequency Of Kappa-Casein And Beta-Lactoglobulin In Indian Crossbred (Bos Taurus X Bos Indicus) Dairy Bulls, Rajesh K. Patel, Jenabhai B. Chauhan, Krishna M. Singh, Kalpesh J. Soni
Turkish Journal of Veterinary & Animal Sciences
A polymerase chain reaction - restriction fragment length polymorphism test was performed on DNA samples extracted from blood samples of 256 Holstein Friesian crossbred and 112 from Jersey crossbred bulls/bull calves to detect allelic frequency of the bovine kappa-casein and beta-lactoglobulin gene responsible for milk production traits. A 350 bp fragment of kappa-casein and a 247 bp fragment of beta-lactoglobulin were amplified and digested with Hinf I and Hae III restriction enzymes, respectively. In the study, 2 types of alleles A and B and 3 types of genotypes AA, BB, and AB for kappa-casein and beta-lactoglobulin were observed. The results …
Left Abdominal Cryptorchidism In A Native Goat, Gülsüm Özyi̇ği̇t
Left Abdominal Cryptorchidism In A Native Goat, Gülsüm Özyi̇ği̇t
Turkish Journal of Veterinary & Animal Sciences
An 18-month-old native goat with 25 kg bodyweight submitted to the Department of Anatomy from a farm for use in student practicals was diagnosed with cryptorchidism. In macroscopical examination, the cryptorchid testis was near the left kidney. The cryptorchid testis was small, firm, and 31 x 30 mm in size and weighed 11 g, while the right testis, located in the scrotum, was 53 x 29 mm in size and weighed 37 g. Microscopically there was tubular hypoplasia and the capsule of the testis (tunica albuginea) was thicker in the left cryptorchid testis. Cryptorchidism is seen more often in horses, …
Effects Of Ulva Rigida And Cystoseira Barbata Meals As A Feed Additive On Growth Performance, Feed Utilization, And Body Composition Of Nile Tilapia, Oreochromis Niloticus, Betül Kut Güroy, Şükran Ci̇ri̇k, Derya Güroy, Feyza Sanver, Ahmet Adem Teki̇nay
Effects Of Ulva Rigida And Cystoseira Barbata Meals As A Feed Additive On Growth Performance, Feed Utilization, And Body Composition Of Nile Tilapia, Oreochromis Niloticus, Betül Kut Güroy, Şükran Ci̇ri̇k, Derya Güroy, Feyza Sanver, Ahmet Adem Teki̇nay
Turkish Journal of Veterinary & Animal Sciences
In a 12-week feeding trial, the effect of 2 algae meals (Ulva rigida or Cystoseira barbata) on feed intake, growth, and nutrient utilization of juvenile Nile tilapia, Oreochromis niloticus, was investigated. This was the first attempt to utilize Cystoseira barbata as a feed supplement for Nile tilapia. The fish were fed to apparent satiation with formulated diets supplemented with various levels of Ulva meal (5%, 10%, or 15%) or Cystoseira meal (5%, 10%, or 15%). A diet without algae meal served as a control diet. The highest values for weight gain were for fish fed the 5% Cystoseira diet, control …
Protein And Lipid Content And Fatty Acid Composition Of Anchovy Meal Produced In Turkey, Hülya Turan, Yalçin Kaya, İbrahi̇m Erkoyuncu
Protein And Lipid Content And Fatty Acid Composition Of Anchovy Meal Produced In Turkey, Hülya Turan, Yalçin Kaya, İbrahi̇m Erkoyuncu
Turkish Journal of Veterinary & Animal Sciences
The aim of this research was to determine the fatty acid composition and lipid and protein levels of anchovy meal produced during the commercial fishing period. The protein content was 74.69%, 76.17% and 74.40% in November, December and January, respectively. The lipid content ranged from 8.57% to 9.14% for anchovy meal produced during the fishing season. The total saturated fatty acid content was 33.25%, 33.46%, and 33.61% in November, December and January, respectively. Within the saturated fraction, the major fatty acid was palmitic acid (C_{16:0}), the content of which was 20.30%, 19.96% and 20.66%, respectively. The monounsaturated fatty acid contents …
Biotyping And Serotyping Of Mannheimia (Pasteurella) Haemolytica Isolated From Lung Samples Of Slaughtered Sheep In The Van Region, Zi̇ya İlhan, İhsan Keleş
Biotyping And Serotyping Of Mannheimia (Pasteurella) Haemolytica Isolated From Lung Samples Of Slaughtered Sheep In The Van Region, Zi̇ya İlhan, İhsan Keleş
Turkish Journal of Veterinary & Animal Sciences
A total of 584 lung samples of slaughtered sheep having clinical symptoms of pneumonia were investigated microbiologically. Out of the 584 samples, 66 (11.3%) Mannheimia (Pasteurella) haemolytica strains were isolated on the basis of cultural, morphological and biochemical characteristics. Sixty-six isolates of M. haemolytica were biotyped by carbohydrate fermentation tests and serotyped by coagglutination and indirect haemagglutination (IHA) tests. Out of the 66 M. haemolytica strains, 57 (86.3%) were detected as biotype A, and 9 (13.6%) were biotype T. The 66 M. haemolytica isolates were serotyped as follows by coagglutination test: 13 (19.6%) A2, 11 (16.6%) A1, 9 (13.6%) A6, …
Unusual Case Of Senecio Sceleratus (Ragwort) Poisoning In An Ostrich (Struthio Camelus) In Zimbabwe, Ross G. Cooper
Unusual Case Of Senecio Sceleratus (Ragwort) Poisoning In An Ostrich (Struthio Camelus) In Zimbabwe, Ross G. Cooper
Turkish Journal of Veterinary & Animal Sciences
No abstract provided.
Cover And Contents
Turkish Journal of Veterinary & Animal Sciences
No abstract provided.
Protective Potential Of Glimepiride And Nerium Oleander Extract On Lipid Profile, Body Growth Rate, And Renal Function In Streptozotocin-Induced Diabetic Rats, Maged M. Yassin, Saleh N. Mwafy
Protective Potential Of Glimepiride And Nerium Oleander Extract On Lipid Profile, Body Growth Rate, And Renal Function In Streptozotocin-Induced Diabetic Rats, Maged M. Yassin, Saleh N. Mwafy
Turkish Journal of Biology
This study aimed to assess the protective potential of glimepiride and Nerium oleander extract on lipid profile, body growth rate, and renal function in streptozotocin-induced diabetic rats. The animals were divided into control and experimental groups. The experimental group was rendered diabetic by intraperitoneal injection of a single dose of 50 mg/kg body weight streptozotocin. Rats with glucose levels >200 mg/dl were subdivided into 3 sub-groups. Rats in the first sub-group remained without treatment and were considered diabetic. Those in the second and third subgroups were orally administered 0.1 mg/kg body weight daily glimepiride and 250 mg/kg body weight daily …
Seroprevalence Of Brucellosis In Human, Sheep, And Cattle Populations In Kırıkkale (Turkey), Teoman Zafer Apan, Murat Yildirim, Ersi̇n İstanbulluoğlu
Seroprevalence Of Brucellosis In Human, Sheep, And Cattle Populations In Kırıkkale (Turkey), Teoman Zafer Apan, Murat Yildirim, Ersi̇n İstanbulluoğlu
Turkish Journal of Veterinary & Animal Sciences
The seroprevalence of Brucellosis in human, sheep, and cattle populations was investigated in rural regions of Kırıkkale in Turkey. Serum samples were obtained from 1436 humans, and 3.2% (46) of the population was found to be positive by Rose Bengal Plate test (RBPT) and 3.0% (43) gave positive results with the standard tube agglutination test (STAT). Immunoglobulin class analysis of RBPT-positive sera using the ELISA test gave positive results for 33 people (75%) with Brucella ELISA IgG and for 11 people (25%) with Brucella ELISA IgM. The evaluation of 804 serum samples, which were obtained from 301 cattle and 503 …
Evaluation Of Two Vaccination Schemes Using Live Vaccines Against Newcastle Disease In Chickens, Mansur-Ud-Din Ahmad, Mamoona Chaudhry, Muhammad Farooq Rai, Hamad Bin Rashid
Evaluation Of Two Vaccination Schemes Using Live Vaccines Against Newcastle Disease In Chickens, Mansur-Ud-Din Ahmad, Mamoona Chaudhry, Muhammad Farooq Rai, Hamad Bin Rashid
Turkish Journal of Veterinary & Animal Sciences
The use of different types of Newcastle disease (ND) vaccine in different vaccination schemes has decreased the incidence of velogenic Newcastle disease (VND) in commercial poultry worldwide. In under-developed countries like Pakistan, these vaccination schemes are not successful due to free-range and backyard poultry production. This trial was conducted in 90 experimental chickens to develop an effective control against ND. The level of antibody response, detected by haemagglutination-inhibition (HI) test, and the degree of protection against the virulent strain of Newcastle disease virus (NDV) were studied. The chickens were immunized with commercially available ND vaccines. In scheme A, primary vaccination …
First Case Of Anaplasma (Ehrlichia) Platys Infection In A Dog In Turkey, Bülent Ulutaş, Göksel Bayramli, Tüli̇n Karagenç
First Case Of Anaplasma (Ehrlichia) Platys Infection In A Dog In Turkey, Bülent Ulutaş, Göksel Bayramli, Tüli̇n Karagenç
Turkish Journal of Veterinary & Animal Sciences
A male pinscher dog weighing 4.2 kg with a history of intermittent fever, inappetence, weight loss, and weakness occurring periodically for 5 months showed Anaplasma (Ehrlichia) platys inclusions within the platelets of peripheral blood smears. The diagnosis was confirmed using a nested polymerase chain reaction. The dog was treated with doxycycline at a dose of 5 mg/kg twice daily for 21 days. This study is the first case of A. platys infection in Turkey.
Estimates Of Genetic Parameters For Racing Times Of Thoroughbred Horses, Bülent Eki̇z, Ömür Koçak
Estimates Of Genetic Parameters For Racing Times Of Thoroughbred Horses, Bülent Eki̇z, Ömür Koçak
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to estimate the genetic parameters for racing times, which are needed for a selection program of Thoroughbred horses in Turkey. The racing records used in the study were obtained from the Turkish Jockey Club. The trait used in the study was racing time for racing distances of 1200, 1300, 1400, 1500, 1600, 1700, 1800, 1900, 2000, 2100, 2200, and 2400 m. The data from each racing distance were analyzed separately. Genetic parameters were estimated by REML procedure using the DFREML program. Estimates of heritability ranged from 0.177 to 0.353, depending on the racing distance. …
Pigmentation Of Rainbow Trout (Oncorhynchus Mykiss) With Carotenoids From Marigold Flower (Tagetes Erecta) And Red Pepper (Capsicum Annum), Hakan Murat Büyükçapar, Mahmut Yanar, Yasemen Yanar
Pigmentation Of Rainbow Trout (Oncorhynchus Mykiss) With Carotenoids From Marigold Flower (Tagetes Erecta) And Red Pepper (Capsicum Annum), Hakan Murat Büyükçapar, Mahmut Yanar, Yasemen Yanar
Turkish Journal of Veterinary & Animal Sciences
The effects of diets including various natural carotenoid sources and synthetic astaxanthin on the pigmentation and growth of rainbow trout (Oncorhynchus mykiss) were investigated. Rainbow trout weighing about 111 g were fed diets containing 1.6%, 2.4% and 3.2% marigold flower; 4.4%, 6.6% and 8.8% red pepper; 100 mg kg^{-1} synthetic astaxanthin; and a control diet for 60 days. At the end of the experiment, synthetic astaxanthin provided the highest carotenoid accumulation in the fish flesh (6.42 mg kg^{-1}) in the groups. This was followed by red pepper (5.78 mg kg^{-1}) and marigold flower (5.59 mg kg^{-1}), each of which included …