Digital Commons Network^™

Synergistic Effects Of Nanosecond Pulsed Electric Fields Combined With Low Concentration Of Gemcitabine On Human Oral Squamous Cell Carcinoma In Vitro, Jing Wang, Jinsong Guo, Shan Wu, Hongqing Feng, Shujun Sun, Jie Pan, Jue Zhang, Stephen J. Beebe

Bioelectrics Publications

Treatment of cancer often involves uses of multiple therapeutic strategies with different mechanisms of action. In this study we investigated combinations of nanosecond pulsed electric fields (nsPEF) with low concentrations of gemcitabine on human oral cancer cells. Cells (Cal-27) were treated with pulse parameters (20 pulses, 100 ns in duration, intensities of 10, 30 and 60 kV/cm) and then cultured in medium with 0.01 mu g/ml gemcitabine. Proliferation, apoptosis/necrosis, invasion and morphology of those cells were examined using MTT, flow cytometry, clonogenics, transwell migration and TEM assay. Results show that combination treatments of gemcitabine and nsPEFs exhibited significant synergistic activities …

Old Dominion University Climate Change And Sea Level Rise Initiative, Summer 2012, Larry P. Atkinson (Editor)

CCSLRI Newsletters

Summer 2012 Newsletter of the Old Dominion University Climate Change and Sea Level Rise Intiative

The Likelihood Of Extinction Of Iconic And Dominant Herbivores And Detritivores Of Coral Reefs: The Parrotfishes And Surgeonfishes, Mia T. Comeros-Raynal, John H. Choat, Beth A. Polidoro, Kendall D. Clements, Rene Abesamis, Matthew T. Craig, Muhammad E. Lazuardi, Jennifer Mcilwain, Andreas Muljadi, Robert F. Myers, Cleto L. Nanola Jr., Shinta Pardede, Luiz A. Rocha, Barry Russell, Jonnell C. Sanciangco, Brian Stockwell, Heather Harwell, Kent E. Carpenter

Biological Sciences Faculty Publications

Parrotfishes and surgeonfishes perform important functional roles in the dynamics of coral reef systems. This is a consequence of their varied feeding behaviors ranging from targeted consumption of living plant material (primarily surgeonfishes) to feeding on detrital aggregates that are either scraped from the reef surface or excavated from the deeper reef substratum (primarily parrotfishes). Increased fishing pressure and widespread habitat destruction have led to population declines for several species of these two groups. Species-specific data on global distribution, population status, life history characteristics, and major threats were compiled for each of the 179 known species of parrotfishes and surgeonfishes …

Sensitivity Of Circumpolar Deep Water Transport And Ice Shelf Basal Melt Along The West Antarctic Peninsula To Changes In The Winds, Michael S. Dinniman, John M. Klinck, Eileen E. Hofmann

CCPO Publications

Circumpolar Deep Water (CDW) can be found near the continental shelf break around most of Antarctica. Advection of this relatively warm water (up to 2 degrees C) across the continental shelf to the base of floating ice shelves is thought to be a critical source of heat for basal melting in some locations. A high-resolution (4 km) regional ocean-sea ice-ice shelf model of the west Antarctic Peninsula (WAP) coastal ocean was used to examine the effects of changes in the winds on across-shelf CDW transport and ice shelf basal melt. Increases and decreases in the strength of the wind fields …

Performance Optimization Over Wireless Links With Operating Constraints, Shiny Abraham

Electrical & Computer Engineering Theses & Dissertations

Wireless communication is one of the most active areas of technological innovations and groundbreaking research ranging from simple cellular phones to highly complex military monitoring devices. The emergence of radios with cognitive capabilities like software defined radios has revolutionized modern communication systems by providing transceivers which can vary their output waveforms as well as their demodulation methods. This adaptability plays a pivotal role in efficient utilization of radio spectrum in an intelligent way while simultaneously not interfering with other radio devices operating on the same frequency band. Thus, it is safe to say that current and future wireless systems and …

Diamond This Film Patterning Technique Using Selective Nucleation Growth, Characterization And Application, Weican Xiao

Electrical & Computer Engineering Theses & Dissertations

Diamond is the hardest bulk material with the highest thermal conductivity. It is also an excellent wide band gap material; hence, diamond is suitable for electronic, mechanical and biological applications. Unfortunately, natural diamond is prohibitively expensive for most technological applications. Polycrystalline diamond, with properties close to those of natural diamond, has been synthesized since the 1950s. Among many methods employed for diamond thin film synthesis, chemical vapor deposition (CVD) using microwave plasma (MW) is quite popular for several reasons. Well-confined plasma that does not touch the reaction chamber walls can be produced directly on the substrate. Hence, contamination in the …

Investigating The Roles Of Time Perspective And Emerging Adulthood In Predicting Driving Behavior, Kristie L. Johnson

Psychology Theses & Dissertations

In the U.S., motor vehicle crashes are the leading cause of death for individuals 2 to 34 years of age (National Highway Traffic Safety Administration, 2009c). Of particular interest are 18 to 25 year olds or emerging adults because of their increased crash risk. The prevalence of crashes attributable to the combination of driving inexperience and risky behaviors creates the necessity to identify predictors of crash likelihoods. While there are known personality variables that predict risky driving, time perspective as an additional one was suggested. Time perspective pertains to how the past, present, and future influence an individual's actions. Zimbardo, …

The Nature And Role Of Physical Models In Enhancing Sixth Grade Students' Mental Models Of Groundwater And Groundwater Processes, Debra Lynne Foster Duffy

Teaching & Learning Theses & Dissertations

Through a non-experimental descriptive and comparative mixed-methods approach, this study investigated the experiences of sixth grade earth science students with groundwater physical models through an extended SE learning cycle format. The data collection was based on a series of quantitative and qualitative research tools intended to investigate students' ideas and changes in ideas rather than measure their achievement. The measures included a groundwater survey, classroom observations, and one-on-one follow-up student interviews for triangulation of data sources. The research was carried out at a K-12 independent school in eastern Virginia using two classes of sixth grade earth science students (n=30).

The …

Teacher Beliefs, Knowledge, And Practice Of Metacognition And Self-Regulated Learning, Robin S. Spruce

Teaching & Learning Theses & Dissertations

Teacher beliefs and knowledge about metacognition and self-regulated learning (SRL) as well as their knowledge for teaching metacognition and SRL were examined in this mixed-methods study. Questionnaires targeting teacher beliefs about SRL, the Self-Regulated Teacher Beliefs Scale (SRLTBS), and teacher knowledge of metacognition and knowledge for teaching metacognition, Teacher Metacognition Scale (TMS), were distributed to elementary and middle school teachers in two school districts, one urban, the other suburban. A total of 84 teachers responded to the questionnaires, and from those a criterion sample of ten were selected for observation and interview. Results from the questionnaires indicate teachers felt somewhat …

Spectroscopic Characterization Of Dissolved Organic Matter: Insights Into Composition, Photochemical Transformation And Carbon Cycling, John Robert Helms

Chemistry & Biochemistry Theses & Dissertations

This dissertation explores processes affecting the composition of dissolved organic matter (DOM) and how DOM composition changes in sunlit surface waters and in the dark interior ocean. Simulated solar irradiations were used to investigate the impact of photochemistry on terrestrial waters and deep ocean DOM. The photochemically mediated processes observed in Dismal Swamp samples included (i) light induced flocculation of up to 12% of the organic matter and 84% of the dissolved iron originally present; (ii) 74-88% mineralization of dissolved organic carbon (DOC) and 95-99% bleaching of chromophoric DOM (CDOM) during 110 days of irradiation; and (iii) nearly complete loss …

The Effects Of An Online Sleep Hygiene Intervention On Students' Sleep Quality, Giuliana Farias

Psychology Theses & Dissertations

Students in college or in their first year of medical school undergo increased educational and social pressure. To cope, students may sacrifice sleep to meet demands. Poor sleep affects learning, performance, and health. Studies have been successful at improving sleep quality through the use of in-person recruitment or cognitive-behavioral therapy delivered over the internet (Trockel, Manber, Chang, Thurston, & Tailor, 2011). The purpose of the current study was to investigate whether an online sleep hygiene intervention could improve sleep quality. One hundred thirty-eight students from one undergraduate institution in Southeast Virginia completed this study. Students were divided into groups; one …

Emotion Management Skills Of School-Age Children In The Context Of Risk: The Role Of Parent Socialization Strategies, Carla Damiani Correia

Psychology Theses & Dissertations

Exposure to risk in childhood can disrupt social and emotional processes (Leventhal & Brooks-Gunn, 2000) and lead to the development of physical and mental health issues across the lifespan (Flouri, 2008). This study sought to better understand the associations between contextual risk, parent socialization of emotion, and children's emotion regulation skills in an at-risk sample. Information about risk was obtained from U.S. Census data and a family questionnaire. Parent socialization strategies and children's ER skills were measured using self-report and interview methods. Though not to the degree that was expected, results indicate that contextual risk relates to both parent socialization …

Evaluation Of Autonomous Robotic Milling Methodology For Natural Tooth-Shaped Implants Based On Sko Optimization, Yongki Yoon

Mechanical & Aerospace Engineering Theses & Dissertations

Robotic surgery is one of the most demanding and challenging applications in the field of automatic control. One of the conventional surgeries, the dental implantation, is the standard methodology to place the artificial tooth root composed of titanium material into the upper or lower jawbone. During the dental implant surgery, mechanical removal of the bone material is the most critical procedure because it may affect the patient's safety including damage to the mandibular canal nerve and/or piercing the maxillary sinus. With this problem, even though short term survival rates are greater than 95%, long term success rate of the surgery …

A Statistical Model To Determine Multiple Binding Sites Of A Transcription Factor On Dna Using Chip-Seq Data, Rasika Jayatillake

Mathematics & Statistics Theses & Dissertations

Protein-DNA interaction is vital to many biological processes in cells such as cell division, embryo development and regulating gene expression. Chromatin Immunoprecipitation followed by massively parallel sequencing (ChIP-seq) is a new technology that can reveal protein binding sites in genome with superior accuracy. Although many methods have been proposed to find binding sites for ChIP-seq data, they can find only one binding site within a short region of the genome. In this study we introduce a statistical model to identify multiple binding sites of a transcription factor within a short region of the genome using the ChIP-seq data. Mapped sequence …

An Exploration Of The State-Trait Continuum In Counseling And Positive Psychology, Michael A. Keefer

Counseling & Human Services Theses & Dissertations

Counselors value remaining positive in the face of adversity. Consequently, positive psychology has placed an emphasis on uncovering how long-enduring positive traits (e.g., hope, wisdom, and creativity) can be developed from short-term positive states. This search has resulted in positive psychology's conceptualization of a state-trait continuum. This study explores the state-trait continuum by examining possible quantitative relationships between a state instrument (the Learning Environment Preferences) and a trait instrument (the Myers-Briggs Type Indicator). Research question 1 found a significant predictive relationship between two MBTI scales (S-N and J-P) and the LEP's cognitive complexity index. Research question 2 found a significant …

De Novo Protein Structure Modeling From Cryoem Data Through A Dynamic Programming Algorithm In The Secondary Structure Topology Graph, Kamal H. Al Nasr

Computer Science Theses & Dissertations

Proteins are the molecules carry out the vital functions and make more than the half of dry weight in every cell. Protein in nature folds into a unique and energetically favorable 3-Dimensional (3-D) structure which is critical and unique to its biological function. In contrast to other methods for protein structure determination, Electron Cryorricroscopy (CryoEM) is able to produce volumetric maps of proteins that are poorly soluble, large and hard to crystallize. Furthermore, it studies the proteins in their native environment. Unfortunately, the volumetric maps generated by current advances in CryoEM technique produces protein maps at medium resolution about (~5 …

Multidentate Resorcinarene Surfactants For The Phase Transfer Of Metal Nanoparticles And Nanodiamonds: Applications In Catalysis And Diamond Film Growth, Vara Prasad Sheela

Chemistry & Biochemistry Theses & Dissertations

One main objective of the present work is to functionalize cuboctahedral platinum nanoparticles with various multidentate resorcinarene surfactants and study their influence in determining their catalytic activity. We hypothesized that catalytically active and recyclable catalysts can be achieved by incomplete yet strong passivation of the nanoparticle surfaces by using multidentate resorcinarene surfactants. We have developed phase transfer protocols for functionalizing cuboctahedral platinum nanoparticles with resorcinarene thiol or amine. Fluorescence assay confirmed that both these nanoparticles contained almost comparable unpassivated metal area. The activity of such phase transferred nanoparticles was tested in the catalytic hydrogenation of allyl alcohol. The conversion of …

Development And Validation Methodology Of The Nuss Procedure Surgical Planner, Krzysztof J. Rechowicz

Computational Modeling & Simulation Engineering Theses & Dissertations

Pectus excavatum (PE) is a congenital chest wall deformity which is characterized, in most cases, by a deep depression of the sternum. A minimally invasive technique for the repair of PE (MIRPE), often referred to as the Nuss procedure, has been proven to be more advantageous than many other PE treatment techniques. The Nuss procedure consists of placement of a metal bar(s) underneath the sternum, thereby forcibly changing the geometry of the ribcage. Because of the prevalence of PE and the popularity of the Nuss procedure, the demand to perform this surgery is greater than ever. Therefore, a Nuss procedure …

Real-Time Virtual Pathology Using Signal Analysis And Synthesis, Dennis L. Bergin

Computational Modeling & Simulation Engineering Theses & Dissertations

This dissertation discusses the modeling and simulation (M& S) research in the area of real-time virtual pathology using signal analysis and synthesis. The goal of this research is to contribute to the research in the M&S area of generating simulated outputs of medical diagnostics tools to supplement training of medical students with human patient role players.

To become clinically competent physicians, medical students must become skilled in the areas of doctor-patient communication, eliciting the patient's history, and performing the physical exam. The use of Standardized Patients (SPs), individuals trained to realistically portray patients, has become common practice. SPs provide the …

Assessing Movement Of Fish Through Spectral Analysis Of Otolith Life History Scans, Renee Reilly Hoover

OES Theses and Dissertations

The ability to accurately measure movement timing across environmental gradients is fundamental for testing hypotheses in marine ecology that deal with ingress, egress, and migration of fish. Timing and patterns of movement have been estimated using life-history scans of the chemical signatures encoded in fish otoliths (ear stones). I provide a quantitative approach to examining life history scan data using spectral analysis, which retrospectively measures the movement timing for individual fish. Sagittal otoliths from juvenile Atlantic croaker (Micropogonias undulates) and adult black sea bass (Centropristis striata) were sampled using laser ablation inductively coupled plasma mass spectrometry …

Identification Of Persistent Long Range Interactions In GA95 And GB95 Through Thermal Unfolding Simulations, Milen Redai Tesfamariam

Chemistry & Biochemistry Theses & Dissertations

For over five decades, different experiments have been performed to research how proteins attain their native three dimensional structures. However, the folding problem continues to be a puzzle in modern science. The design of two proteins that have maximal sequence identity but different folds and functions is one method that is being used to study the relationship between protein structure and amino acid sequence. In particular, mutant proteins of Streptococcus protein G, G_A and G_B, have 95% sequence identity and a 3a helix fold and β4/a fold, respectively. Molecular dynamics simulations of G_A95 …

All Is Fair In War---Violent Conflict And The Securitization Of Rape, Sabine Hirschauer

Graduate Program in International Studies Theses & Dissertations

Systematic mass rape during conflict has for centuries met with global ignorance and political complacency despite its atrocious character. The conflicts in Bosnia and Rwanda in the 1990s altered the silence surrounding this long tolerated, dark underbelly of war. Applying Barry Buzan's and Ole Waever'sSecuritization Theory to the cases of Bosnia and Rwanda, this dissertation finds that rape during both wars was successfully securitized. Securitization Theory as a model and indicator of an effective shifting of rape from an apolitical or political perspective into the security realm was appropriate. Bosnia initiated the first convictions of rape as a …

Multilevel Analysis Of Physical Activity Among Us Adults Across Us Census Regions: The Role Of Environmental Contexts, Sariyamon Tiraphat

Health Services Research Dissertations

This study uses nationally representative data to explain the variation in physical activity among U.S. adults across four geographic regions of Northeast, Midwest, South, and West. The purpose of the study is to investigate whether environmental conditions inclusive of natural amenity, built environment, urbanization, crime, and social economic neighborhood are associated with physical activity and whether these associations are modified by geographic region. The study also investigates whether the influences of environmental contexts vary by subgroup and whether they are modified by geographic region. Determining regional factors that influence physical activity is essential to planning appropriate physical activity interventions.

The …

Population Structure And Connectivity Of An Important Pelagic Forage Fish In The Antarctic Ecosystem, Pleuragramma Antarcticum In Relation To Large Scale Circulation, Jason W. Ferguson

OES Theses and Dissertations

Ocean circulation has been identified as a major process controlling the distribution of biological material in marine systems. Large-scale transport by the Antarctic Circumpolar Current (ACC), the Ross and Weddell Gyres, and the Antarctic Coastal Current can promote spatially complex population structure in the Southern Ocean through advection. Antarctic silverfish (Pleuragramma antarcticum), a pelagic, neutrally buoyant notothenioid fish species, are distributed around the shelf systems of Antarctica and are considered an important species rivaling krill as prey for many birds, seals, whales, and other fish. We asked whether silverfish are distributed in independent, discrete populations along the shelf systems of …

90th Annual Meeting Of The Virginia Academy Of Science: Proceedings

Virginia Journal of Science

Full proceedings of the 90th Annual Meeting of the Virginia Academy of Science, Norfolk State University, Norfolk Virginia, May 23-25, 2012

Securing South Africa's Future Grandmothers Against Poverty And Aids As A Model For Social Development Change, Savannah Lynn Eck

Graduate Program in International Studies Theses & Dissertations

Focusing on the role of elder women in South Africa as a lens to understand the central connections among HIV/ AIDS, poverty and Human Security provides a distinct approach to analyze women's contributions to community development and social change. Drawing from the theories of Gender and Development and Human Security, this research aims to highlight HIV/ AIDS as a social and political security issue, while underscoring the vitality of the inclusion of women in the processes of peace building, reconciliation, education and social development. Furthermore, the influential role of elder women in South Africa will serve as a model in …

Evaluation Of Electrophoretic Protein Extraction And Database-Driven Protein Identification From Marine Sediments, Eli K. Moore, Brook L. Nunn, Jessica F. Faux, David R. Goodlett, H. Rodger Harvey

OES Faculty Publications

Intact proteins comprise a major component of organic carbon and nitrogen produced globally and are likely an important fraction of organic matter in sediments and soils. Extracting the protein component from sediments and soils for mass spectral characterization and identification represents a substantial challenge given the range of products and functionalities present in the complex matrix. Multiple forms of gel electrophoresis were evaluated as a means of enhancing recovery of sedimentary protein before proteomic characterization and compared with a direct enzymatic digestion of proteins in sediments. Resulting tryptic peptides were analyzed using shotgun proteomics and tandem mass spectra were evaluated …

Art Therapy Assessment Of Adult Incarcerated Offenders: A Research Proposal For The Assessment Of Locus Of Control, Alecia Perreault

EVMS School of Health Professions Theses & Dissertations

There is a lack of research that combines both art therapy assessment and prison inmates as it relates to locus of control. This is a research proposal that attempts to highlight the need for research with art therapy assessments among the offender population. More specifically, it attempts to correlate art therapy assessments with the assessment of specific traits, like locus of control, as they are typically found in offenders. This thesis reviews literature that is related to the field of art therapy assessment, projective drawing assessment, and the offender population, as well as discusses implications for further study.

Effects Of Temperature On Emergence And Seasonality Of West Nile Virus In California, David M. Hartley, Christopher M. Barker, Arnaud Le Menach, Tianchan Niu, Holly Gaff, William K. Reisen

Biological Sciences Faculty Publications

Temperature has played a critical role in the spatiotemporal dynamics of West Nile virus transmission throughout California from its introduction in 2003 through establishment by 2009. We compared two novel mechanistic measures of transmission risk, the temperature-dependent ratio of virus extrinsic incubation period to the mosquito gonotrophic period (BT), and the fundamental reproductive ratio (R₀) based on a mathematical model, to analyze spatiotemporal patterns of receptivity to viral amplification. Maps of BT and R₀ were created at 20-km scale and compared throughout California to seroconversions in sentinel chicken flocks at half-month intervals. Overall, estimates of BT and …

Exploring Hope And Quality Of Life: A Proposal For A Group Art Therapy Hope Intervention For Individuals Diagnosed With Lupus, Paige Scheinberg

EVMS School of Health Professions Theses & Dissertations

As a result of the importance of hope and quality of life in disease management for individuals who have been diagnosed with lupus, there is support in the literature for the need for an effective intervention that targets these constructs with this population. The efficacy of art therapy in meeting the physical, mental, and emotional needs of diverse populations is also supported in existing research, making this an ideal form of treatment for lupus patients. In an effort to fill a gap in the literature on the relationship(s) between lupus, hope, and art therapy, this study was created. The original …