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Imprinting Of An Evolutionarily Conserved Antisense Transcript Gene Apeg3, Jung Ha Choo, Jeong Do Kim, Joomyeong Kim Feb 2008

Imprinting Of An Evolutionarily Conserved Antisense Transcript Gene Apeg3, Jung Ha Choo, Jeong Do Kim, Joomyeong Kim

Faculty Publications

APeg3 is an antisense transcript gene of Peg3, which has been recently identified from rat brain. Careful analyses of EST databases indicated that a homologous transcript also exists in other mammalian species, including mouse, cow and human. 5′-and 3′-RACE experiments have subsequently identified a 900-bp cDNA sequence of APeg3 from mouse brain. Mouse APeg3 is localized in the 3′UTR of Peg3 with an intronless genomic structure. The expression of mouse APeg3 is derived mainly from the paternal allele, indicating the imprinting of this antisense transcript gene in brain. Strand-specific RNA analyses also revealed the expression of both human and cow …

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The Psb27 Protein Facilitates Manganese Cluster Assembly In Photosystem Ii, Johnna L. Roose, Himadri B. Pakrasi Feb 2008

The Psb27 Protein Facilitates Manganese Cluster Assembly In Photosystem Ii, Johnna L. Roose, Himadri B. Pakrasi

Faculty Publications

Photosystem II (PSII) is a large membrane protein complex that uses light energy to convert water to molecular oxygen. This enzyme undergoes an intricate assembly process to ensure accurate and efficient positioning of its many components. It has been proposed that the Psb27 protein, a lumenal extrinsic subunit, serves as a PSII assembly factor. Using a psb27 genetic deletion strain (Δpsb27) of the cyanobacterium Synechocystis sp. PCC 6803, we have defined the role of the Psb27 protein in PSII biogenesis. While the Psb27 protein was not essential for photosynthetic activity, various PSII assembly assays revealed that the Δpsb27 mutant was …

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Origin Of The Eastern Brownsnake, Pseudonaja Textilis (Duméril, Bibron And Duméril) (Serpentes: Elapidae: Hydrophiinae) In New Guinea: Evidence Of Multiple Dispersals From Australia, And Comments On The Status Of Pseudonaja Textilis Pughi Hoser 2003, David J. Williams, Mark O'Shea, Roland L. Daguerre, Catharine E. Pook, Wolfgang Wüster, Christopher J. Hayden, John D. Mcvay, Owen Paiva, Teatu Lohi Matainaho, Kenneth D. Winkel, Christopher C. Austin Feb 2008

Origin Of The Eastern Brownsnake, Pseudonaja Textilis (Duméril, Bibron And Duméril) (Serpentes: Elapidae: Hydrophiinae) In New Guinea: Evidence Of Multiple Dispersals From Australia, And Comments On The Status Of Pseudonaja Textilis Pughi Hoser 2003, David J. Williams, Mark O'Shea, Roland L. Daguerre, Catharine E. Pook, Wolfgang Wüster, Christopher J. Hayden, John D. Mcvay, Owen Paiva, Teatu Lohi Matainaho, Kenneth D. Winkel, Christopher C. Austin

Faculty Publications

Pseudonaja textilis is a widespread and common snake in eastern parts of Australia, but its distribution in New Guinea is poorly understood, and the origin of the New Guinea populations and its timing have been the subject of much speculation. Phylogenetic analysis of mitochondrial DNA sequences from three New Guinea populations of P. textilis indicates that New Guinea was colonised from two independent eastern and western migration routes most likely in the Pleistocene. One dispersal event from northern Queensland led to the populations in eastern New Guinea (Milne Bay, Oro and Central Provinces, Papua New Guinea), whereas another, from Arnhem …

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A Unified Association Analysis Approach For Family And Unrelated Samples Correcting For Stratification, Xiaofeng Zhu, Shengchao Li, Richard S. Cooper, Robert C. Elston Feb 2008

A Unified Association Analysis Approach For Family And Unrelated Samples Correcting For Stratification, Xiaofeng Zhu, Shengchao Li, Richard S. Cooper, Robert C. Elston

Faculty Publications

There are two common designs for association mapping of complex diseases: case-control and family-based designs. A case-control sample is more powerful to detect genetic effects than a family-based sample that contains the same numbers of affected and unaffected persons, although additional markers may be required to control for spurious association. When family and unrelated samples are available, statistical analyses are often performed in the family and unrelated samples separately, conditioning on parental information for the former, thus resulting in reduced power. In this report, we propose a unified approach that can incorporate both family and case-control samples and, provided the …

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In Vivo Yy1 Knockdown Effects On Genomic Imprinting, Joomyeong Kim, Jeong Do Kim Feb 2008

In Vivo Yy1 Knockdown Effects On Genomic Imprinting, Joomyeong Kim, Jeong Do Kim

Faculty Publications

The YY1 transcription factor is predicted to control several imprinted domains, including the Peg3, Gnas and Xist/Tsix regions. To test this possibility, we have used RNA interference strategies to generate transgenic mouse lines that express reduced levels of the cellular YY1 protein. As predicted, lowering YY1 levels resulted in global expression changes in these three imprinted domains. In neonatal brains, most imprinted genes of the Peg3 domain were up-regulated. In the Gnas domain, Nespas was down-regulated, whereas three other imprinted transcripts were up-regulated, including Nesp, Gnasxl and Exon1A. In the Xist/Tsix domain, no obvious change was detected in the expression …

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Interactions Between Bacterial Carbon Monoxide And Hydrogen Consumption And Plant Development On Recent Volcanic Deposits, Gary M. King, Carolyn F. Weber Feb 2008

Interactions Between Bacterial Carbon Monoxide And Hydrogen Consumption And Plant Development On Recent Volcanic Deposits, Gary M. King, Carolyn F. Weber

Faculty Publications

Patterns of microbial colonization and interactions between microbial processes and vascular plants on volcanic deposits have received little attention. Previous reports have shown that atmospheric CO and hydrogen contribute significantly to microbial metabolism on Kilauea volcano (Hawaii) deposits with varied ages and successional development. Relationships between CO oxidation and plant communities were not clear, however, since deposit age and vegetation status covaried. To determine plant-microbe interactions in deposits of uniform ages, CO and hydrogen dynamics have been assayed for unvegetated tephra on a 1959 deposit at Puu Puai (PP-bare), at the edge of tree islands within the PP deposit (PP-edge) …

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Cellular Uptake And Lysosomal Delivery Of Galactocerebrosidase Tagged With The Hiv Tat Protein Transduction Domain, Xian Yang Zhang, Annie Dinh, James Cronin, Su Chen Li, Jakob Reiser Feb 2008

Cellular Uptake And Lysosomal Delivery Of Galactocerebrosidase Tagged With The Hiv Tat Protein Transduction Domain, Xian Yang Zhang, Annie Dinh, James Cronin, Su Chen Li, Jakob Reiser

Faculty Publications

A number of studies have shown that a short peptide, the protein transduction domain (PTD) derived from the HIV-1 Tat protein (Tat-PTD) improved cellular uptake in vitro and distribution in vivo of recombinant proteins bearing such PTDs when administered systemically. To investigate the effects of Tat-PTD addition on the subcellular localization of the lysosomal enzyme galactocerebrosidase (GALC, EC 3.2.2.46) and with a view towards designing improved therapeutic strategies for Krabbe disease (globoid cell leukodystrophy), mouse GALC was tagged C-terminally with the Tat-PTD. Compared with unmodified GALC, GALC bearing a Tat-PTD, a myc epitope and 6 consecutive His residues [GALC-TMH (Tat-PTD, …

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Determination Of Pharmacologically Active Compounds In Root Extracts Of Cassia Alata L. By Use Of High Performance Liquid Chromatography, Vivian E. Fernand, David T. Dinh, Samuel J. Washington, Sayo O. Fakayode, Jack N. Losso, Rick O. Van Ravenswaay, Isiah M. Warner Jan 2008

Determination Of Pharmacologically Active Compounds In Root Extracts Of Cassia Alata L. By Use Of High Performance Liquid Chromatography, Vivian E. Fernand, David T. Dinh, Samuel J. Washington, Sayo O. Fakayode, Jack N. Losso, Rick O. Van Ravenswaay, Isiah M. Warner

Faculty Publications

A simple high performance liquid chromatography (HPLC) method was developed and validated for the determination of six phenolic compounds, five anthraquinones (rhein, aloe-emodin, emodin, chrysophanol and physcion) and a flavonoid (kaempferol), in root extracts from Cassia alata L. Solid-phase extraction, using C18 cartridges, was used to remove interfering substances from the root extracts. The extracts were analyzed on a C18 column using an isocratic mobile phase which consisted of acetonitrile, methanol, and 10 mM aqueous ammonium acetate (25:55:20, v/v). Identification of the analytes was performed by use of standards and on-line mass spectrometric detection using atmospheric pressure chemical ionization. The …

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Bis(1-Tosyl-2-Pyrrolyl)Ethyne, Hillary K. Tanui, Frank R. Fronczek, M. Graça H. Vicente Jan 2008

Bis(1-Tosyl-2-Pyrrolyl)Ethyne, Hillary K. Tanui, Frank R. Fronczek, M. Graça H. Vicente

Faculty Publications

The title mol-ecule, C24H20N2O4S2, has crystallographic inversion symmetry with a triple-bond distance of 1.206 (2) Å. The alkyne is not quite linear, with a C - C C angle of 175.78 (16)°. The planar pyrrole rings are parallel but offset from coplanarity by 0.318 (1) Å. The conformation of the sulfonyl group with respect to the pyrrole ring is such that an O atom is nearly eclipsed with this ring, having an O - S - N - C torsion angle of 3.48 (11)°. C - H⋯O inter-actions [C⋯O 3.278 (2) Å, 136° about H] between pyrrole H and sulfonyl …

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A Threading-Based Method (Findsite) For Ligand-Binding Site Prediction And Functional Annotation, Michal Brylinski, Jeffrey Skolnick Jan 2008

A Threading-Based Method (Findsite) For Ligand-Binding Site Prediction And Functional Annotation, Michal Brylinski, Jeffrey Skolnick

Faculty Publications

The detection of ligand-binding sites is often the starting point for protein function identification and drug discovery. Because of inaccuracies in predicted protein structures, extant binding pocketdetection methods are limited to experimentally solved structures. Here, FINDSITE, a method for ligand-binding site prediction and functional annotation based on binding-site similarity across groups of weakly homologous template structures identified from threading, is described. For crystal structures, considering a cutoff distance of 4 Å as the hit criterion, the success rate is 70.9% for identifying the best of top five predicted ligand-binding sites with a ranking accuracy of 76.0%. Both high prediction accuracy …

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Effects Of Eutrophication On Salt Marsh Root And Rhizome Biomass Accumulation, F A. Darby, R. Eugene Turner Jan 2008

Effects Of Eutrophication On Salt Marsh Root And Rhizome Biomass Accumulation, F A. Darby, R. Eugene Turner

Faculty Publications

The root:shoot ratio of grassland plants may be lower in high fertility sites than in low fertility sites as plants modify their root, rhizome and shoot morphologies (and masses) to suit prevailing nutrient availability. We conducted geographically diverse and regionally specific field sampling and measured above- and belowground plant biomasses in western Atlantic and Gulf of Mexico salt marshes to determine whether there is a similar morphological response in Spartina alterniflora, the dominant salt marsh plant. Coastal nutrient addition/enrichment, which is widespread and ongoing, may lower root and rhizome biomass, belowground production and organic accumulation in this species. Higher soil …

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Ocean Urea Fertilization For Carbon Credits Poses High Ecological Risks, P.M. Glibert, R. Azanza, M. Burford, K. Furuya, E. Abal, A. Al-Azri, F. Al-Yamani, P. Andersen, D.M. Anderson, J. Beardall, G.M. Berg, L. Brand, D. Bronk, J. Brookes, J.M. Burkholder, A. Cembella, W.P. Cochlan, J.L. Collier, Y. Collos, R. Diaz, M. Doblin, T. Drennen, S. Dyhrman, Y. Fukuyo, M. Furnas, J. Galloway, E. Granéli, D.V. Ha, G. Hallegraeff, J. Harrison, P.J. Harrison, C.A. Heil, K. Heimann Jan 2008

Ocean Urea Fertilization For Carbon Credits Poses High Ecological Risks, P.M. Glibert, R. Azanza, M. Burford, K. Furuya, E. Abal, A. Al-Azri, F. Al-Yamani, P. Andersen, D.M. Anderson, J. Beardall, G.M. Berg, L. Brand, D. Bronk, J. Brookes, J.M. Burkholder, A. Cembella, W.P. Cochlan, J.L. Collier, Y. Collos, R. Diaz, M. Doblin, T. Drennen, S. Dyhrman, Y. Fukuyo, M. Furnas, J. Galloway, E. Granéli, D.V. Ha, G. Hallegraeff, J. Harrison, P.J. Harrison, C.A. Heil, K. Heimann

Faculty Publications

No abstract provided.

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Gumbo Yearbook, Class Of 2008, Louisiana State University And Agricultural And Mechanical College Jan 2008

Gumbo Yearbook, Class Of 2008, Louisiana State University And Agricultural And Mechanical College

Gumbo Yearbook

The Gumbo yearbook chronicles the entire academic year at LSU. In words and especially photos, the Gumbo shows the people, places, and events that make each year unique. In addition to formal portraits of schools and departments, the book contains hundreds of snapshots of students with their friends and dozens of pages showcasing organizations.

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Multiple Yy1 And Ctcf Binding Sites In Imprinting Control Regions, Joomyeong Kim Jan 2008

Multiple Yy1 And Ctcf Binding Sites In Imprinting Control Regions, Joomyeong Kim

Faculty Publications

Known imprinting control regions (ICRs) contain unusual tandem arrays of DNA-binding sites for transcription factors, including YY1 for the Peg3, Gnas and Xist/Tsix domains and CTCF for the H19/Igf2 domain. These multiple DNA-binding sites are known to be the only functionally shared and evolutionarily selected feature among these ICRs. However, it is not well understood why the imprinting control regions tend to maintain a high density of a particular transcription factor-binding site. We hypothesize that the multiplicity associated with the YY1 and CTCF binding sites may be designed for attracting and maintaining the relatively high levels of YY1 and CTCF …

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Beaf Regulates Cell-Cycle Genes Through The Controlled Deposition Of H3k9 Methylation Marks Into Its Conserved Dual-Core Binding Sites., Eldon Emberly, Roxane Blattes, Bernd Schuettengruber, Magali Hennion, Nan Jiang, Craig M. Hart, Emmanuel Käs, Olivier Cuvier Jan 2008

Beaf Regulates Cell-Cycle Genes Through The Controlled Deposition Of H3k9 Methylation Marks Into Its Conserved Dual-Core Binding Sites., Eldon Emberly, Roxane Blattes, Bernd Schuettengruber, Magali Hennion, Nan Jiang, Craig M. Hart, Emmanuel Käs, Olivier Cuvier

Faculty Publications

Chromatin insulators/boundary elements share the ability to insulate a transgene from its chromosomal context by blocking promiscuous enhancer-promoter interactions and heterochromatin spreading. Several insulating factors target different DNA consensus sequences, defining distinct subfamilies of insulators. Whether each of these families and factors might possess unique cellular functions is of particular interest. Here, we combined chromatin immunoprecipitations and computational approaches to break down the binding signature of the Drosophila boundary element-associated factor (BEAF) subfamily. We identify a dual-core BEAF binding signature at 1,720 sites genome-wide, defined by five to six BEAF binding motifs bracketing 200 bp AT-rich nuclease-resistant spacers. Dual-cores are …

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School Of Renewable Natural Resources Newsletter, Fall 2008, Louisiana State University And Agricultural & Mechanical College Jan 2008

School Of Renewable Natural Resources Newsletter, Fall 2008, Louisiana State University And Agricultural & Mechanical College

Research Matters & RNR Newsletters

The annual newsletter from the School of Renewable Natural Resources was a publication which aimed to update and entertain interested readers with the news and research from RNR faculty, students and staff.

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Description Of The Larva Of Pselaphophus Atriventris (Staphylinidae: Pselaphinae: Pselaphini) With Notes On Its Life History And A List Of Described Pselaphine Immature Stages, Christopher E. Carlton, Richard A.B. Leschen Jan 2008

Description Of The Larva Of Pselaphophus Atriventris (Staphylinidae: Pselaphinae: Pselaphini) With Notes On Its Life History And A List Of Described Pselaphine Immature Stages, Christopher E. Carlton, Richard A.B. Leschen

Faculty Publications

Approximately 8,900 species of the staphylinid beetle subfamily Pselaphinae have been described, based on adults, but the larvae of only 18 species and 14 genera have been described in sufficient detail for systematic study. The larva of Pselaphophus atriventris (Westwood) (Staphylinidae: Pselaphinae: Pselaphini) is described herein based on a series collected in a pasture habitat in New Zealand. Larvae are distinguished from other described larvae of pselaphines based on the following combination of characters: antennal segment two bearing two trifid setiform sensoria and labrum with irregularly arranged short spines and setiferous tubercles. It is most similar to the larva of …

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Integrating Phylogenetic And Population Genetic Analyses Of Multiple Loci To Test Species Divergence Hypotheses In Passerina Buntings, Matt D. Carling, Robb T. Brumfield Jan 2008

Integrating Phylogenetic And Population Genetic Analyses Of Multiple Loci To Test Species Divergence Hypotheses In Passerina Buntings, Matt D. Carling, Robb T. Brumfield

Faculty Publications

Phylogenetic and population genetic analyses of DNA sequence data from 10 nuclear loci were used to test species divergence hypotheses within Passerina buntings, with special focus on a strongly supported, but controversial, sister relationship between Passerina amoena and P. caerulea inferred from a previous mitochondrial study. Here, a maximum-likelihood analysis of a concatenated 10-locus data set, as well as minimize-deep-coalescences and maximum-likelihood analyses of the locus-specific gene trees, recovered the traditional sister relationship between P. amoena and P. cyanea. In addition, a more recent divergence time estimate between P. amoena and P. cyanea than between P. amoena and P. caerulea …

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Optimization Of 12 Chiral Analytes With 8 Polymeric Surfactants, Eugene Billiot, Fereshteh Billiot, Isiah M. Warner Jan 2008

Optimization Of 12 Chiral Analytes With 8 Polymeric Surfactants, Eugene Billiot, Fereshteh Billiot, Isiah M. Warner

Faculty Publications

This manuscript discusses the results of studies that were performed to determine optimum capillary electrophoresis (CE) conditions for the enantiomeric resolution of twelve chiral analytes with eight amino acid based polymeric surfactants. The parameters that were optimized include pH, buffer type, and concentration of surfactant. The results indicated that the optimum conditions for enantiomeric separations with the amino acid based polymeric surfactants examined in this study using CE were analyte dependent, not surfactant dependent. In other words, the optimum conditions for a particular analyte were the same for all the amino acid based polymeric surfactants examined in this study. The …

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Entry Approach Into Pristine Ice-Sealed Lakes-Lake Vida, East Antarctica, A Model Ecosystem, Peter T. Doran, Christian H. Fritsen, Alison E. Murray, Fabien Kenig, Chris P. Mckay, Jay D. Kyne Jan 2008

Entry Approach Into Pristine Ice-Sealed Lakes-Lake Vida, East Antarctica, A Model Ecosystem, Peter T. Doran, Christian H. Fritsen, Alison E. Murray, Fabien Kenig, Chris P. Mckay, Jay D. Kyne

Faculty Publications

Ice-sealed lakes, potentially home to novel microbiota and microbial processes, can provide a window into isolated and geologically ancient systems. These habitats are earth analogs for extraterrestrial systems that have yet to be sampled, though potentially harbor, or have harbored life at some time during their past. They are also small-scale models of the numerous sub-glacial lake systems, which have been identified across Antarctica and in Iceland. Methods are needed to sample these ecosystems with environmental stewardship in mind, in which human impact on the ecosystem is mitigated before and during sampling. This report describes an entry and sampling approach …

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Dna Inhibits Catalysis By The Carboxyltransferase Subunit Of Acetyl-Coa Carboxylase: Implications For Active Site Communication, Brian K. Benson, Glen Meades, Anne Grove, Grover L. Waldrop Jan 2008

Dna Inhibits Catalysis By The Carboxyltransferase Subunit Of Acetyl-Coa Carboxylase: Implications For Active Site Communication, Brian K. Benson, Glen Meades, Anne Grove, Grover L. Waldrop

Faculty Publications

Acetyl-CoA carboxylase (ACC) catalyzes the first committed step in the synthesis of long-chain fatty acids. The crystal structure of the Escherichia coli carboxyltransferase component of ACC revealed an α2b 2 subunit composition with two active sites and, most importantly, a unique zinc domain in each ab pair that is absent in the eukaryotic enzyme. We show here that carboxyltransferase binds DNA. Half-maximal saturation of different single-stranded or double-stranded DNA constructs is seen at 0.5-1.0 μM, and binding is cooperative and nonspecific. The substrates (malonyl-CoA and biocytin) inhibit DNA:carboxyltransferase complex formation. More significantly, single-stranded DNA, double-stranded DNA, and heparin inhibit the …

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Temperature And Vegetation Effects On Soil Organic Carbon Quality Along A Forested Mean Annual Temperature Gradient In North America, Cinzia Fissore, Christian P. Giardina, Randall K. Kolka, Carl C. Trettin, Gary M. King, Martin F. Jurgensen, Christopher D. Barton, S. Douglas Mcdowell Jan 2008

Temperature And Vegetation Effects On Soil Organic Carbon Quality Along A Forested Mean Annual Temperature Gradient In North America, Cinzia Fissore, Christian P. Giardina, Randall K. Kolka, Carl C. Trettin, Gary M. King, Martin F. Jurgensen, Christopher D. Barton, S. Douglas Mcdowell

Faculty Publications

Both climate and plant species are hypothesized to influence soil organic carbon (SOC) quality, but accurate prediction of how SOC process rates respond to global change will require an improved understanding of how SOC quality varies with mean annual temperature (MAT) and forest type. We investigated SOC quality in paired hardwood and pine stands growing in coarse textured soils located along a 22 °C gradient in MAT. To do this, we conducted 80-day incubation experiments at 10 and 30 °C to quantify SOC decomposition rates, which we used to kinetically define SOC quality. We used these experiments to test the …

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Central And Peripheral Administration Of Kisspeptin Activates Gonadotropin But Not Somatotropin Secretion In Prepubertal Gilts, Clay A. Lents, Neely L. Heidorn, C. Richard Barb, J. Joe Ford Jan 2008

Central And Peripheral Administration Of Kisspeptin Activates Gonadotropin But Not Somatotropin Secretion In Prepubertal Gilts, Clay A. Lents, Neely L. Heidorn, C. Richard Barb, J. Joe Ford

Faculty Publications

It is well established that kisspeptin signaling is necessary for the onset of puberty in laboratory animals. However, the role that kisspeptin may have in regulating puberty in large domestic animals is unknown. We tested the hypothesis that either central or peripheral infusion of kisspeptin would stimulate gonadotropin and GH secretion in prepubertal gilts. In experiment 1, prepubertal gilts were fitted with i.c.v. cannula and indwelling jugular catheters. Animals were randomly assigned to receive 0, 10, or 100 μg kisspeptin in saline. In experiment 2, prepubertal gilts, fitted with indwelling jugular catheters, randomly received 0, 1, 2.5, or 5 mg …

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Components, Volume 4 (2008), Lsu Center For Computation & Technology Jan 2008

Components, Volume 4 (2008), Lsu Center For Computation & Technology

Components

No abstract provided.

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Phonotaxis To Amphibian Vocalizations In Culex Territans (Diptera: Culicidae), Kristen Bartlett-Healy, Wayne Crans, Randy Gaugler Jan 2008

Phonotaxis To Amphibian Vocalizations In Culex Territans (Diptera: Culicidae), Kristen Bartlett-Healy, Wayne Crans, Randy Gaugler

Faculty Publications

Culex territans Walker (Diptera: Culicidae) acquires bloodmeals from amphibian hosts. Females overwinter as inseminated adults and exit diapause in New Jersey when spring peepers (Pseudacris crucifer) are calling. We tested the hypothesis that Cx. territans uses amphibian vocalizations as a long-distance attractant. Two thirds of females oriented toward sound across all experiments. Females allowed to orient toward or away from a frog call, bird song, live frog, or control (a plugged in compact disc player) exhibited positive phonotaxis only to the frog call. Females exhibited positive phonotaxis to calls of P. crucifer, Hyla versicolor (northern gray tree frog), Bufo americanus …

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The Termite Menace In New Orleans: Did They Cause The Floodwalls To Tumble?, Gregg Henderson Jan 2008

The Termite Menace In New Orleans: Did They Cause The Floodwalls To Tumble?, Gregg Henderson

Faculty Publications

No abstract provided.

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Uncovering A Macrophage Transcriptional Program By Integrating Evidence From Motif Scanning And Expression Dynamics, Stephen A. Ramsey, Sandy L. Klemm, Daniel E. Zak, Kathleen A. Kennedy, Vesteinn Thorsson, Bin Li, Mark Gilchrist, Elizabeth S. Gold, Carrie D. Johnson, Vladimir Litvak, Garnet Navarro, Jared C. Roach, Carrie M. Rosenberger, Alistair G. Rust, Natalya Yudkovsky, Alan Aderem, Ilya Shmulevich Jan 2008

Uncovering A Macrophage Transcriptional Program By Integrating Evidence From Motif Scanning And Expression Dynamics, Stephen A. Ramsey, Sandy L. Klemm, Daniel E. Zak, Kathleen A. Kennedy, Vesteinn Thorsson, Bin Li, Mark Gilchrist, Elizabeth S. Gold, Carrie D. Johnson, Vladimir Litvak, Garnet Navarro, Jared C. Roach, Carrie M. Rosenberger, Alistair G. Rust, Natalya Yudkovsky, Alan Aderem, Ilya Shmulevich

Faculty Publications

Macrophages are versatile immune cells that can detect a variety of pathogen-associated molecular patterns through their Toll-like receptors (TLRs). In response to microbial challenge, the TLR-stimulated macrophage undergoes an activation program controlled by a dynamically inducible transcriptional regulatory network. Mapping a complex mammalian transcriptional network poses significant challenges and requires the integration of multiple experimental data types. In this work, we inferred a transcriptional network underlying TLR-stimulated murine macrophage activation. Microarray-based expression profiling and transcription factor binding site motif scanning were used to infer a network of associations between transcription factor genes and clusters of co-expressed target genes. The time-lagged …

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"Almost Impossible In Praise" Dedicatory Criticism In English Dramatic Texts Of The Seventeenth Century, David Roberts Jan 2008

"Almost Impossible In Praise" Dedicatory Criticism In English Dramatic Texts Of The Seventeenth Century, David Roberts

1650-1850: Ideas, Aesthetics, and Inquiries in the Early Modern Era

No abstract provided.

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Unnatural Conjugation In Tristram Shandy, Book Vii, Arnd Bohm Jan 2008

Unnatural Conjugation In Tristram Shandy, Book Vii, Arnd Bohm

1650-1850: Ideas, Aesthetics, and Inquiries in the Early Modern Era

No abstract provided.

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Strategies For Commercialization Of Cryopreserved Fish Semen, Terrence R. Tiersch Jan 2008

Strategies For Commercialization Of Cryopreserved Fish Semen, Terrence R. Tiersch

Faculty Publications

Initial success in sperm cryopreservation occurred at about the same time for aquatic species and livestock. However, in the 50 plus years since then cryopreserved sperm of livestock has grown into a billion-dollar global industry, while cryopreserved sperm of aquatic species remains a research activity with little commercial application despite work in more than 90 species and more than 200 published reports. Most research work has focused on large-bodied culture and sport fishes, such as salmon, trout, carp, and catfish, and mollusks such as commercially important oyster and abalone species. However, only a few studies have addressed sperm cryopreservation in …

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