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Evolution Of Yy1, Yy2, Rex1 And Dna-Binding Motifs In Vertebrate Genomes, Christopher Don Faulk

LSU Doctoral Dissertations

Transcription factors are important for many aspects of gene regulation in eukaryotes. YY1 (Yin-Yang 1) is a particularly interesting example of a highly conserved zinc-finger transcription factor, involved in transcriptional activation, repression, initiation, and in chromatin modification. YY1 is ubiquitously expressed in mammals, and its binding sites are found in ~10% of human genes as well as in repetitive elements. It is a targeting protein of the Polycomb complex and is involved in mammalian genomic imprinting. First, we explored the evolutionary history of YY1 using 62 species and formation of its paralogs, YY2 and REX1, which are found in mammals, …

A Study Of The Elongation Of Fibrils Of The Alzheimer's Amyloid Beta Protein With A Coarse-Grained Force Field, Ana Rojas

LSU Doctoral Dissertations

Alzheimer’s disease (AD) is a brain disorder named after the German psychiatrist Alois Alzheimer, who first described the disease in 1906. AD is a form of dementia, characterized by impairment of memory and other intellectual abilities, caused by the loss of neurons and synapses (the structures that permit communication between neurons) in certain regions of the brain. Although the mechanism by which neurons are affected is not yet well understood, research points to a small protein, the beta-amyloid peptide (Abeta), as the first suspect. Ab chains are present in the brains of healthy individuals, but in AD patients, they associate …

Iron (Iii) Oxide And Copper (Ii) Oxide Mediated Formation Of Pcdd/Fs From Thermal Degradation Of 2-Mcp And 1,2-Dcbz, Shadrack Kirwa Nganai

LSU Doctoral Dissertations

Formation of polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) which occurs inexorably from most thermal and combustion processes constitutes a major toxic component of environmental pollutants. Generally, it is well established that transition metal-mediated reactions account for the majority of PCDD/F emissions from combustion sources. Specifically, both copper and iron ions, which occur abundantly in combustion generated particulate matter, are considered probably the most active in promoting PCDD/F formation typically in the low temperature post-combustion zone and flue gas pollution control devices. It has also been demonstrated that chlorinated phenols are key intermediates in essentially all pathways of PCDD/F formation. Chlorinated benzenes …

Artisanal Whaling In The Atlantic: A Comparative Study Of Culture, Conflict, And Conservation In St. Vincent And The Faroe Islands, Russell Fielding

LSU Doctoral Dissertations

Whalers from the Caribbean island of St. Vincent and the North Atlantic archipelago of the Faroe Islands hunt pilot whales and a variety of other small cetaceans for food. Vincentian whalers use harpoons, thrown by hand or fired from a modified shotgun mounted on the boat. Faroese whalers, using several dozen boats, work cooperatively to drive an entire pod of whales ashore, where shore-based whalers are waiting to complete the kill with traditional whaling knives. Vincentian whaling traces its origins to the late nineteenth century. Records of Faroese whaling date to the late sixteenth century but the practice is thought …

The Conserved Deda Family Of E. Coli Membrane Proteins: Genetic And Topological Analysis, Kandi Sheral Thompkins

LSU Doctoral Dissertations

Ludox density gradients were used to enrich for Escherichia coli mutants with conditional growth defects and alterations in membrane composition. A temperature-sensitive mutant named Lud135 was isolated with mutations in two related, nonessential genes: yghB and yqjA. yghB harbors a single missense mutation (G203D) and yqjA contains a nonsense mutation (W92TGA) in Lud135. Both mutations are required for the temperature-sensitive phenotype: targeted deletion of both genes in a wild-type background results in a strain with a similar phenotype and expression of either gene from a plasmid restores growth at elevated temperatures. The mutant has altered membrane phospholipid levels, with elevated …

Population Genetics And Systematics Of A Species-Rich Clade Of Neotropical Reef Fishes, The Tubeblenny Genus Acanthemblemaria, Ron Israel Eytan

LSU Doctoral Dissertations

Neotropical coral reef fish communities are species-poor compared to those of the Indo-West Pacific. An exception to that pattern is the blenny clade Chaenopsidae, one of only three coral reef fish families endemic to the Neotropics. Within the chaenopsids, the genus Acanthemblemaria is the most species-rich. To understand the origin and maintenance of genetic and species diversity in these fishes, I characterized the population genetics for two Acanthemblemaria species, reconstructed the phylogeny of the group, and identified suites of correlated morphological characters responsible for the distinctive skull morphology of these fishes. By combining nuclear and mitochondrial sequence data I was …

Effects Of Habitat Structural Complexity On Nekton Assemblages: Lab And Field Observations In Southern Louisiana, Austin T. Humphries

LSU Master's Theses

Greater structural complexity is often associated with more diverse and abundant species assemblages. Biogenic reefs formed by the eastern oyster (Crassostrea virginica) are structurally complex in nature and have been recognized for their potential habitat value in estuarine systems along the Atlantic and Gulf of Mexico coasts. To determine how the structural complexity of newly created oyster reefs may influence the abundance and distribution of species, three objectives were established. First, to examine spatial and temporal patterns of nekton use at newly created oyster reefs, as well as the impact of wave exposure, six paired oyster reef and mud-bottom treatments …

Implementation And Analysis Of A Top-K Retrieval System For Strings, Sabrina Chandrasekaran

LSU Master's Theses

Given text which is a union of d documents of strings, D = d1, d2,...., dd, the emphasis of this thesis is to provide a practical framework to retrieve the K most relevant documents for a given pattern P, which comes as a query. This cannot be done directly, as going through every occurrence of the query pattern may prove to be expensive if the number of documents that the pattern occurs in is much more than the number of documents (K) that we require. Some advanced query functionality will be required, as compared to listing the documents that the …

The Feminization Of Private Investigation: A Sociological Analysis, Jessica Simpson Pearce

LSU Doctoral Dissertations

This dissertation outlines the occupation of private investigation and the role that females play within that profession. The difficulties women experience in male-oriented occupations remains noteworthy in sociological research today. Progress has been made, yet many barriers still exist for women. These include structural, social, and cultural factors that influence women and/or the jobs that they hold. With the completion of interviews with twenty-six female private investigators, I was able to analyze the existing structural, interactional, and gender barriers which moderate the number of women that work as private investigators. Few studies have examined this occupation and this gap may …

Edaphic And Vegetative Controls On Mercury Cycling In Oligohaline Wetlands, Jonathan M. Willis

LSU Doctoral Dissertations

With the expansion of the human population and associated industries there is a concomitant increase in both resource utilization and the production of waste and deleterious by-products. Mercury is a naturally-occurring toxic metal with a complicated and unique biogeochemical cycle, and is often a contaminant of ecotoxicological concern in unindustrialized aquatic habitats. The research described herein was designed to elucidate multiple aspects regarding the behavior of mercury in oligohaline wetland habitats, particularly with respect to edaphic and vegetative effects, through a monitoring and characterization study as well as a series of manipulative experiments. The observational study, conducted in the wetlands …

Parent Education In A Child Welfare Setting: Understanding Maltreatment Following An Intervention For Parents And Their Infants, Toddlers, And Pre-School Children, Rhenda Hotard Hodnett

LSU Doctoral Dissertations

Child abuse and neglect is a complex, multi-faceted problem that often has lifelong, negative consequences for its victims; most significantly affecting infants, toddlers and pre-school age children. Parenting classes are the most common intervention used by child welfare agencies as a means to prevent repeat maltreatment, yet there is very little research involving these targeted families. Prior research has primarily focused on the prevalence of and risk factors for child maltreatment, with much less attention on specific parenting program outcomes as implemented in a child welfare setting. In 2005, focusing on a more deliberate and systematic approach in the use …

Model Based Analysis Of Some High Speed Network Issues, Suman Kumar

LSU Doctoral Dissertations

The study of complex problems in science and engineering today typically involves large scale data, huge number of large-scale scientific breakthroughs critically depends on large multi-disciplinary and geographically-dispersed research teams, where the high speed network becomes the integral part. To serve the ongoing bandwidth requirement and scalability of these networks, there has been a continuous evolution of different TCPs for high speed networks. Testing these protocols on a real network would be expensive, time consuming and more over not easily available to the researchers worldwide. Network simulation is well accepted and widely used method for performance evaluation, it is well …

Seventh-Grade Curriculum In Probability (A Guide For Teachers), David Nganga Njenga

LSU Master's Theses

A review of the literature indicates that teachers try to make connections between experimental and theoretical probabilities while teaching their students, but these connections are often not very clear. This greatly increases misconceptions students have about probability. This thesis presents a treatment of teaching probability that is geared for seventh grade and intended to minimize the misconceptions that both teachers and students may have. We present a concise mathematical exposition of finite probability models as well as collection of examples and activities, so as to help teachers and students organize their thinking and minimize misconceptions. The various examples and activities …

Effects Of Serum Addition To Culture Medium On Gene Expression In Day-7 And Day-14 Bovine Embryos, Jaime Manuel Angulo Campos

LSU Master's Theses

The addition of serum to embryo culture media may alter gene expression and trigger development of Large Offspring Syndrome. The objectives of this study were to determine gene expression levels in embryos cultured in the absence or presence of 5% calf serum and compare these expression patterns to in vivo derived embryos (IVD), and to determine the effects of serum on the length of day-14 embryos. Abattoir derived oocytes were fertilized and cultured in mSOFaa. At 72 hours post-insemination (hpi), embryos were randomly allocated into two treatments: mSOFaa without and with 5% calf serum. Embryos were then cultured to 168 …

Macular Pigment Optical Density During Pregnancy And Its Relationship To The Diet, Alicia Page

LSU Master's Theses

Women, compared to men, are at higher risk for age-related macular degeneration (AMD), the leading cause of blindness in the elderly. The accumulation of dietary carotenoids, lutein (L) and zeaxanthin (Z) within the macula make up macular pigment and provide important protection to the retina. Macular pigment optical density (MPOD) gives a measure of macula pigment density. MPOD has been shown to be influenced by fruit and vegetable L and Z and docosahexanoic acid (DHA, 22:6n-3), cold water marine fish being the dietary source. A high MPOD may protect against AMD. Intake of DHA by women is low (40-120 mg/d) …

Design And Development Of Chiral And Achiral Molecularly Imprinted Stationary Phases, Jason Paul Lejeune

LSU Doctoral Dissertations

Although the cross-linker can comprise over 80% of the polymer composition, improving the nature of the cross-linker in molecularly imprinted polymers has not been studied extensively. The goal of this research is to develop novel cross-linking monomers to either use in the One MoNomer Molecularly Imprinted Polymer system (OMNiMIP) or use in conjunction with other commercially available cross-linkers and functional monomers. Chapter 2 contains research into the understanding of the performance of a new cross-linking monomer (N, O - bismethacryloyl ethanolamine, NOBE) discovered in the Spivak Research Group. The ability of this monomer to outperform traditional two monomer systems in …

Urate Responsive Marr Homologs, Inoka Chinthana Perera

LSU Doctoral Dissertations

Differential gene expression in response to internal and external stimuli is studied in detail to understand the intricate mechanisms underlying response to various environmental stressors in microorganisms. MarR family transcriptional regulators have been studied for their involvement in such mechanisms. This work elucidates the mechanism of urate-induced attenuation of DNA binding of HucR, a MarR homolog, and extends this mechanism to describe a novel subfamily of MarR homologs responsive to urate, proposing a physiological relevance of utilizing urate as a signaling molecule.

HucR (hypothetical urate regulator) binds to the shared promoter region between uricase and hucR genes. It has high …

The Role Of Old-Fashioned Racism: Disaggregating Symbolic Racism In The United States, Leslie Curtis Cox

LSU Master's Theses

Old-fashioned, biological, or "Jim Crow" racism is viewed by many in the political science and psychology literature to be largely a relic of the past. In the post-segregation era it has been replaced as a political force by symbolic racism, although its residual effect still operates within symbolic racism as negative racial affect. Symbolic racism is thought of as a coherent belief system that describes whites‘ attitudes not only in the United States, but in some European democracies as well. This conceptualization of symbolic racism ignores the differences in the historical legacy of racism across different regional and demographic contexts. …

Revealing The Role Of Receptor Wsx1: A Double-Edged Sword In Tumor Progession, Denada Dibra

LSU Doctoral Dissertations

Tumor initiation and progression are dependent on both aberrant gene expression in tumor cells and the communication between tumor cells and its micro- and systemic microenvironment. Many tumor suppressor genes and oncogenes have been characterized to suppress or promote tumor growth, but fewer genes in tumors are well-characterized as interacting with immune cells in the host to promote or inhibit tumor growth. The interleukin (IL) 27 receptor WSX1 is expressed in immune cells and induces an IL27-dependent immune response. Opposing this conventional dogma, our initial results reveal a much higher level of WSX1 expression in multiple types of epithelial tumor …

Solid-Liquid Separation Technologies In The Conversion Of Bagasse To Liquid Fuel, Keith Miller

LSU Master's Theses

Development of liquid biofuels has entered a new phase of large scale pilot demonstrations. A number of plants in operation or under construction face the engineering challenges of creating a viable plant design, scaling up, and optimizing various unit operations. It is well-known that separation technologies account for 50-70% of both capital and operating costs. Processes vary in terms of selection of unit operations; however, solid-liquid separations are likely to be a major contributor to the overall project costs. A typical process for ethanol production from biomass includes several solid-liquid separation steps. The nature of biomass derived materials makes it …

Effects Of Meal Timing On Anabolic Hormone Status And Energy Metabolism In Neonatal Dairy Calves, Katherine Claire Simon

LSU Master's Theses

Twelve neonatal Holstein bull calves (38.52 ± 5.87 kg) were fed milk replacer at a fixed or varied meal time to determine the effects on metabolic hormone secretion, average daily intake, growth, and energy metabolism. Body weights were measured every two weeks from birth to 8 weeks. Rumen fluid was collected every two weeks from week 2 through 8. Serial blood collections were conducted every two weeks from week 2 through 8. Blood was collected, beginning at 0530, at 0, 15, 30, 45, 60, 75, 90, 105, 120, 135, and 150 minutes. Plasma was analyzed for ghrelin, leptin, growth hormone …

Hydrogen Production From Molasses Using Chlorinated Alkane-Contaminated Groundwater As An Inoculum, Hector Narez

LSU Master's Theses

The Scenic Highway area of the PetroProcessors of Louisiana, Inc. (PPI) Superfund site located near Baton Rouge, LA, has groundwater contaminated with a variety of chlorinated alkanes (e.g., 1,2-dichloroethane, 1,2-dichloropropane) and alkenes (e.g., trichloroethene and vinyl chloride). In March 2009, a field-scale pilot test was conducted to evaluate the efficacy of injecting diluted agricultural feed grade molasses into the groundwater in an effort to stimulate in-situ reductive dechlorination of these chlorinated contaminants. Because H2 plays an important role as an electron donor in the metabolism of reductively dechlorinating bacteria, in support of the pilot-scale effort, experiments described in this thesis …

Modulation Of Cholinergic Signaling In Embryonic Zebrafish: Anatomical And Behavioral Consequences, Evdokia Menelaou

LSU Doctoral Dissertations

Smoking during pregnancy remains a major health concern in the United States. Nicotine is one of the main ingredients of cigarettes and a neurotoxicant which has been purported to affect many aspects of nervous system development causing long-term abnormalities in the offspring. We took advantage of the zebrafish model to investigate the effects of nicotine in nervous system anatomy and function. It was previously reported that embryonic nicotine exposure causes pathfinding errors in secondary motoneuron (SMN) axons through the activation of nicotine acetylcholine receptors (nAChRs). However, the nicotine-induced effects on SMN pathfinding coincided with muscle degeneration. We hypothesized that nicotine …

Sorption Mechanisms Of Zinc In Different Clay Minerals And Soil Systems As Influenced By Various Natural Ligands, Mohammed Hashem Stietiya

LSU Doctoral Dissertations

The bioavailability and fate of Zinc (Zn) in soils is influenced by reactions occurring at the water-mineral interface. Understanding Zn interaction with mineral surfaces is essential to the understanding of Zn fate and toxicity. In this study, adsorption experiments investigated the impact of ligands and pH on the adsorption of Zn to mineral surfaces. X-Ray Absorption Fine Structure Spectroscopy (XAFS) was used to elucidate the adsorption mechanisms of Zn to mineral surfaces as impacted by ligands. Impact of ligands on Zn adsorption was dependent on mineral type and pH of the system. XAFS analysis showed that adsorption mechanisms of Zn …

Synthesis And Characterization Of Rare Earth-Nickel-Gallium Ternary Intermetallics, Kandace R. Thomas

LSU Doctoral Dissertations

The structural and physical characterization of several early and latter rare earth Ln-Ni-Ga systems, which include Ln2NiGa12 (Ln = Pr, Nd, Sm), alpha-LnNiGa4 (Ln = Y, Gd – Yb) and beta-LnNiGa4 (Ln = Tb – Ho) will be presented in this work. These systems are thermodynamically located within a copious, robust phase space and provide a rich understanding of how slight modifications to synthetic preparations can yield the adoption of different structure types in a Ga-rich regime. Each of these phases is made up of well-studied substructures which lend an additional angle of apperception as to how their structure and …

Pharmaceutical Compounds In Treatment Wetlands: Potential Removal And Effects On Microbial Processes, Jeremy Landon Conkle

LSU Doctoral Dissertations

active compounds (PhACs) have been detected in the aquatic environment as a result of loading from various sources. In Louisiana, USA, many municipalities treat wastewater using natural systems, such as lagoons and wetlands, rather than using conventional wastewater treatment technologies and may discharge PhACs into the environment. These treatment systems are not designed to remove PhACs from wastewater, nor is it currently a regulatory requirement. Research on the fate of PhACs in the environment is needed to understand impacts on Louisiana’s important coastal system. Wastewater sampling for PhACs at the Mandeville, LA wastewater treatment plant (WWTP) determined that this system …

Structure And Organization Of Canopy Bird Assemblages In Lowland Neotropical Rainforests, David Lawrence Anderson

LSU Doctoral Dissertations

Although birds of the forest canopy are an important component of tropical forest ecosystems, difficulty accessing the forest canopy has limited the advancement of knowledge pertaining to this group of species. Here I test methods for the study of canopy birds in lowland Neotropical rainforests, and identify recurring patterns of community structure in canopy bird assemblages as well as processes potentially responsible for these patterns. I used three methods to assess differences in ground-based and canopy-based methods for detecting forest birds in a 100-ha plot of lowland rainforest in northern Honduras: (1) point counts from the ground; (2) repeat censuses …

Caring Attitudes Among Child Welfare Caseworkers: Associations With Client Participation In Services, Karen Faulk

LSU Doctoral Dissertations

The purpose of this study was to determine if there is an association between the caring attitudes and commitment toward clients of child welfare workers and their clients’ completion of a parenting education program. This line of inquiry is intended to expand the scope of research on caring attitudes associated with child welfare workers intent to remain employed. A logical extension of identifying characteristics associated with child welfare workforce retention is to determine if those characteristics are also associated with positive client outcomes. Part of the examination of worker caring attitudes involved testing the Child Welfare Inventory (CWI), a modified …

The Roles Of Transcription Factors In Nucleotide Excision Repair In Yeast, Baojin Ding

LSU Doctoral Dissertations

Nucleotide excision repair (NER) is a conserved DNA repair mechanism capable of removing a variety of helix-distorting lesions, such as UV-induced cyclobutane pyrimidine dimers (CPDs). NER can be grouped into two pathways: global genomic NER (GGR), which refers to repair throughout the genome, and transcription coupled NER (TCR), which refers to a repair mechanism that is dedicated to the transcribed strand (TS) of actively transcribed genes. In yeast S. cerevisiae, Rad7, Rad16, and Elc1 are specifically required for GGR. TCR is believed to be initiated by RNA polymerase II (Pol II) stalled at a lesion in the TS of a …

The Care Plan As An Indicator Of Change In Nursing Science Instruction: A Textbook-Based Analysis, Lindsay Bratton-Mullins

LSU Doctoral Dissertations

Sciences are critical in nursing education to aid the nurse in understanding health and disease processes. Accrediting bodies for nursing education have emphasized that educators teach in ways that encourage critical thinking and, therefore, produce safe-practicing, competent nurse graduates. Nursing care plans best reflect nursing education’s central goals. Because of its longevity of use and familiarity, in this study, the nursing care plan was used as a proxy for nursing science’s learning objectives. This research was a study of the nursing care plan as an indicator for change in nursing science education in the United States to determine if change …