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Highly Efficient Selection, Enumeration, Enrichment, And Molecular Profiling Of Low-Abundance Biological Cells, Udara R. Dharmasiri Rasika Dharmasiri

LSU Doctoral Dissertations

After brief overviews of low-abundance cell selection techniques in chapter 1 and circulating tumor cells in chapter 2, this dissertation initially focuses on the development of aptamer incorporated high-throughput microfluidic techniques to select rare circulation prostate cancer cells (LNCaP) directly from whole blood with subsequent quantification of these rare cells using a non-labeling approach. Then, I extended the technology to environmental samples in an effort around time, sensitivity, and portability of traditional groundwater assessment. As a model bio- pathogen, E. coli O157:H7 was chosen due to its toxicity and its adverse impact on recreational waters. Low-abundance (<100 cells mL-1) E. coli O157:H7 cells were isolated and enriched from environmental water samples using a microfluidic chip that its capture beds were covalently decorated with E.coli O157:H7 specific polyclonal antibodies. The selected cells were enumerated using RT-qPCR technique. Finally, I have integrated HTMSU with electrokinetic enrichment microfluidic unit for performance of single recombinant low-abundance CTC cell-based assay. A series of analytical processes were carried out, including immunoaffinity selection of rare CTCs, quantification of selected cells via conductivity impedance and electrophoretic enrichment of selected cells for PCR/LDR/CE interrogation for detection of low-abundance point mutations in genomic DNA.

The Effect Of Anisotropic Surface Energy On The Stability Of Micro And Nano Wires, Ping Du

LSU Doctoral Dissertations

A liquid thread of radius R will break up into drops if the axial wavelength of the surface perturbation L > 2πR. If L < 2πR, the thread is stable and will remain intact. This is Rayleigh’s stability criterion based on a continuum model. We use molecular dynamics to simulate the evolution of Lennard-Jones liquid threads with equilibrium radius R = 2.3-6.6, where R has been non-dimensionalized by the distance at which the Lennard-Jones potential equals zero. We find that if R is fixed, the wavelength L is bounded by L_min and L_max. For L > L_max the thread always breaks up and stays as drops, and for L < L_min the thread remains intact. However, for L_min < L < L_max, the thread oscillates continuously among several shapes. The appearance of various shapes can be explained by the energy fluctuation of the system.

We also simulate the evolution of Lennard-Jones nanowires with equilibrium radius R = 1.57, 2.58, 3.59, and 4.60 by molecular dynamics. The wires …

Fabrication Of Receptor-Modified Microfluidic Surfaces For Applications In Glycoprotein Screening, Jennifer Macalindong De Guzman

LSU Doctoral Dissertations

Glycoproteins have long been identified to have a profound association with human pathological processes, and they are much sought after as potential biomarkers to aid in the early diagnosis and clinical prognosis of cancers and diseases. There is currently high demand for high-throughput and low–limit–of–detection techniques that can afford profiling of the glycoproteome. Micro-total analysis systems (µTAS) based on microfluidics have the potential to fulfill these requirements, but in order to reduce the complexity of the protein pool, the µTAS devices must contain a pre-isolation and enrichment component. The research project undertaken here involved derivatization of microfluidic …

Positron Autoradiography For Intravascular Vulnerable Plaque Imaging, Peter Petrek

LSU Master's Theses

Purpose: To develop and investigate an intravascular storage phosphor detector for imaging and detection of vulnerable coronary artery plaques. Methods: The concept of the detection of vulnerable plaques (i.e., plaques with inflammation) is based on accumulation of fluorodeoxyglucose (FDG) in inflammation sites. The FDG is labeled with a radioactive isotope 18F (positron source), injected to patient, and accumulated in vulnerable plaques of coronary arteries. The radiation detector is then inserted into coronary artery, records positrons from FDG accumulated in vulnerable plaque, which allows for imaging and detection of the vulnerable plaque. A prototype detector was developed and tested. The detector …

Revitalizing The Suburban Dream: Disaster, Displacement And Resilience In Eastern Orleans Parish, Meredith Morgan Feike

LSU Doctoral Dissertations

Hurricane Katrina tested the disaster resiliency of communities throughout the city of New Orleans. More than four years after breaches in levees led to the flooding of one hundred forty square miles of the Crescent City, some residents are still struggling to reclaim their neighborhoods one block at a time. Eastern Orleans Parish is a portion of the local landscape whose long-term sustainability remains uncertain. Rebuilding is extremely slow with only a fraction of residents returning to the area. Development in New Orleans East began after World War II as a result of urban sprawl. Many New Orleanians desired a …

Reliability And Validity Study Of The Motiviation For Fear (Motif) Survey, Marie S. Nebel-Schwalm

LSU Doctoral Dissertations

The aim of this study was to determine psychometric properties of a newly created, 24-item functional measure of fear and anxiety for typically-developing adults (the Motivation for Fear; MOTIF). Participants initially included 1,277 college students ranging in age from 18-35. Participants were asked to complete the MOTIF, the Questions About Behavioral Function (QABF), the Sensation Seeking Scale- Form V, and the Depression Anxiety Stress Scales (DASS). Analyses were conducted on those scoring above a minimum threshold on a well-normed measure of anxiety (the DASS). An exploratory factory analysis, using scree plot and parallel analysis, as well as oblique rotation was …

Diatom Stratigraphy Defining Ice Sheet Grounding Chronology, Ross Sea, Antarctica, Juan Manuel Chow

LSU Doctoral Dissertations

Evidence of Antarctic ice sheet behavior and paleoenvironment is often approached from proxy records because there is a dearth of direct records from Antarctica itself. The studies presented here seek to augment our knowledge of translations of the West Antarctic Ice Sheet’s (WAIS) marine termination based on direct biostratigraphic evidence. Diatom analyses were conducted from two areas of Ross Sea, Antarctica. In the first study, diatoms were used to constrain the age of diamictons from five seismic units in the Eastern Basin and thus expand our knowledge of WAIS behavior during the Southern Hemisphere Last Glacial Maximum (LGM). All five …

Reading Out Of Doors: How Nature Becomes Text And Vice-Versa, Richmond Minor Eustis

LSU Doctoral Dissertations

The tensions between city and country, the artificial and the natural, the real and the fake are at the heart of attempts to render nature in writing. In many such texts, nature—especially wilderness—is the realm of the real, authentic, and pure, while the city is the realm of the artificial and corrupt. This placement of value in nature, members of the critical theory camp tend to counter, is misguided. Any effort to render nature in text is by its “nature” artificial—far more about human values embedded in language itself than about some extra-textual world. With an approach derived from theorists …

Other Tomorrows: Postcoloniality, Science Fiction And India, Suparno Banerjee

LSU Doctoral Dissertations

In this dissertation I argue that science fiction as a genre intervenes in the history-oriented discourse of postcolonial Anglophone Indian literature and refocuses attention on the nation’s future—its position in global politics, its shifting religious and social values, its rapid industrialization, the clash between orthodoxy and modernity, and ultimately the dream of a multicultural nation. Anglophone Indian science fiction also indicates India’s movement away from a nation trying to negotiate the stigma of colonialism to a nation emerging as a new world power. Thus, this genre reconstructs the Indian identity not only in the domestic sphere, but also in a …

Modeling And Simulation For Shape Memory Polymer Based Self-Healing Syntactic Foam, Wei Xu

LSU Doctoral Dissertations

Syntactic foams, renowned for their low density and high mechanical properties, are enjoying continuing growth in various civilian and military sectors. However, like laminated composites, foam cored structures are vulnerable to impact damages and suffering from inabilities in repairing macro-scale cracks. A self-healing mechanism for structural damage is genuinely desired. A recent development in self-healing structural damage is a two-step close then heal (CTH) scheme proposed by Li and Nettles [1] and elucidated by Li and Uppu [2], by mimicking the self-healing process of human skin. This concept has been further demonstrated in Nji and Li’s work [3] that a …

Oxidation Of Reduced Sulfur Compounds: A Triple-Oxygen-Isotope Perspective, Issaku E. Kohl

LSU Doctoral Dissertations

The Earth’s geochemical evolution is recorded in the rocks that compose its lithosphere. Specifically, sulfate minerals have been identified as being repositories of information concerning the past hydrosphere, atmosphere and biosphere. This is due to the non-labile nature of SO42- and its ability to store a record of the oxidative reactions and oxygen sources involved in its formation. Microbial dissimilatory sulfate reduction (MDSR) and sulfide oxidation cause oxygen from H2O and O2 to be trapped to varying degrees in ambient, dissolved SO42-. In order to better interpret the H2O and O2 signals in SO42-, we must deepen our understanding of …

Modeling And Multiresolution Characterization Of Micro/Nano Surface For Novel Tailored Nanostructures, Rajib Mukherjee

LSU Doctoral Dissertations

Nanofabrication is state of the art technology. Various chemical, mechanical, biochemical and semiconductor products have characteristics controlled by the nanostructures of the surface and interphase. Surface microscopic imaging is generally used to capture different surface features. By properly analyzing the surface image, valuable information regarding manufacturing process and product performance can be extracted. While microscopy measurements can offer very accurate qualitative information about surface features, for many applications, it is critical to obtain a quantitative description of the surface morphology. Various statistical features can be used to characterize the surface in quantitative way. Such an analysis can be done by …

The Effect Of War On Art: The Work Of Mark Rothko, Elizabeth Leigh Doland

LSU Master's Theses

My goal for this thesis was to adequately illustrate the effect war can have on art and artists. I chose to single out one particular artist who lived and worked during a time of war and explore his life and work. My choice of artist was not random: I chose an individual who was particularly concerned about his external environment, and was active in the political and social issues of the time. My subject is Mark Rothko, a Russian-Jewish artist who immigrated from Russia as a boy and spent his life in the hotspot of artistic inspiration, New York City. …

Study Of Host-Fungus Interactions Between Soybean And Phakopsora Pachyrhizi Using Proteomics, Sunjung Park

LSU Doctoral Dissertations

Asian soybean rust, caused by Phakopsora pachyrhizi, is an emerging disease in the continental U. S. and resistant commercial varieties have not been reported. In an effort to understand the interactions during rust infection of soybean, protein profile changes were examined over a 14-day period in soybean leaves of one susceptible commercial line (Pioneer 93M60) with or without soybean rust inoculation using proteomics in this study. Forty protein spots differentially expressed after rust inoculation were identified and fourteen of them were recovered and sequenced. These included proteins involved in plant defense, stress, metabolism, and other biological processes. During the time-course …

In Vivo And In Vitro Pathogenesis Of Francisella Asiatica In Tilapia Nilotica (Oreochromis Niloticus), Esteban Soto

LSU Doctoral Dissertations

Francisella asiatica is a Gram negative, facultative intracellular pathogen that causes fish francisellosis. In this project, homologues to the F. tularensis iglABCD genes were found present in F. asiatica. As few as 23 F. asiatica bacteria injected in the peritoneum were found capable of causing mortalities in tilapia nilotica (Oreochromis niloticus), and even fewer were enough to cause pathological changes. We examined the in vivo and in vitro interaction of F. asiatica wild type (WT) and a ÄiglC strain with tilapia and tilapia head kidney derived macrophages (HKDM). The ÄiglC was found to be attenuated following intraperitoneal and immersion challenges …

Writing About The South "In Her Own Way": Gender And Region In The Work Of Southern Women Playwrights, Casey Kayser

LSU Doctoral Dissertations

This dissertation examines how identity—gender, race, sexuality, regional affiliation—intersects with considerations of the dramatic genre, commercial and critical factors in the American theatre, and understandings about the American South to complicate how contemporary southern women playwrights represent region. In light of the always-already "performative" nature of the South, and geographical, commercial, and ideological factors that set the South in opposition to the North, southern women playwrights face additional difficulties in navigating issues of authenticity and simulacra, the universal versus the specific, ideas about southern "backwardness" versus northern sophistication, and audience participation in fetishizing or distancing the South. Using drama as …

A Taxometric Analysis Of Autism Spectrum Disorders In Toddlers, Jessica Ann Boisjoli

LSU Doctoral Dissertations

Autism Spectrum Disorders (ASD) are a group of neurodevelopmental disorders that are generally first diagnosed in childhood. With the advances in technology to identify this group of disorders, children are being identified at younger and younger ages. Early identification of ASD is critical due to the beneficial effects of early intensive behavioral interventions. While children are being diagnosed with the disorder at very high rates, etiology and definitions of the disorders are still being investigated. Great variability exists with regard to symptoms between individuals. Additionally, less is known about symptom expression in individuals without ASD and symptoms of ASD occurring …

Landscape Patterns Of Community Structure, Biomass And Net Primary Productivity Of Mangrove Forests In The Florida Coastal Everglades As A Function Of Resource, Regulators, Hydroperiod, And Hurricane Distrubance, Edward Castaneda

LSU Doctoral Dissertations

Spatial and temporal patterns of mangrove vegetation in the Florida Coastal Everglades (FCE) reflect a major interplay of resources, regulators and hydroperiod gradients. I investigated landscape patterns of community structure, biomass and NPP of mangroves along two FCE estuaries: Shark River and Taylor River. I also evaluated whether pulsing events such as Hurricane Wilma are significant to soil nutrient inventories and vertical accretion of mangroves in FCE. There was a higher forest structural complexity of mangroves in Shark River relative to Taylor River. The biomass root:shoot ratio was 17 times higher in Taylor River relative to Shark River, indicating that …

Joep Franssens' Harmony Of The Spheres: A Conductor's Analysis, David Andrew Hobson

LSU Doctoral Dissertations

Harmony of the Spheres is Dutch composer Joep Franssens’ most extensive choral work to date, exhibiting a substantial possibility to enter the international repertoire as a complete work; however, several of the movements can stand alone effectively. Presented in five symmetrically conceived movements, Franssens scores the piece for SSAATTBB chorus with only the addition of full string orchestra for Movement III. The composition seeks to explore profound connections between science, music, philosophy, religion, and human relationships, intertwining excerpts from Benedict de Spinoza’s magnum opus, Ethica, allusions to the ancient idea of the music of the spheres, and European minimalism. Franssens …

In Vitro And In Vivo Evaluation Of A Brucella Putative Hemagglutinin, Lauren E. Duhon

LSU Doctoral Dissertations

Brucellosis, a zoonotic disease caused by Brucella spp., presents both health and economic difficulties for livestock, wildlife, and humans. While brucellosis is nearly eradicated in the United States, the disease remains detrimental in many countries worldwide. Attempts to produce a safe and effective small ruminant vaccine have been met with limited success. The current vaccine for bovine brucellosis in the United States is B. abortus RB51. This strain transiently colonizes the host and induces a cell-mediated immune response. Levels of protection have not been demonstrated in goats and thus it is considered a relatively poor caprine vaccine that probably does …

"Teach Us Incessantly": Lessons And Learning In The Antebellum Gulf South, Sarah L. Hyde

LSU Doctoral Dissertations

Before 1860 people in the Gulf South valued education and sought to extend schooling to residents across the region. Southerners learned in a variety of different settings – within their own homes taught by a family member or hired tutor, at private or parochial schools as well as in public free schools. Regardless of the venue, the ubiquity of learning in the region reveals the importance of education in Southern culture. In the 1820s and 1830s, legislators in Louisiana, Mississippi, and Alabama sought to increase access to education by offering financial assistance to private schools in order to offset tuition …

Dissolution Of Chitosan In Ionic Liquids For Acylation Chemistry And Formation Of Cellulose-Chitosan Blends, Cristina Stefanescu

LSU Doctoral Dissertations

Dissolution of chitosan in ionic liquids was accomplished. It has been shown that 1-butyl-3-methylimidazolium acetate (BMIMAc) is a better solvent than 1-butyl-3-methylimidazolium chloride (BMIMCl). Dissolution of chitosan in BMIMCl required a prior regeneration of chitosan from 1% acetic acid solution. In the case of BMIMAc, both dried chitosan and regenerated chitosan from acetic acid solution have been dissolved in a relatively short amount of time. While concentrations up to 2 wt% of chitosan in BMIMCl could be obtained, concentrations of 10 wt % of chitosan in BMIMAc were realized. Homogeneous phthalation and benzoylation of chitosan were achieved in these ionic …

The Effects Of A Freshwater Diversion Of Nekton Species Biomass Distributions, Food Web Pathways, And Community Structure In A Louisiana Estuary, Kim De Mutsert

LSU Doctoral Dissertations

A current method to restore Louisiana’s estuaries includes reintroducing freshwater and sediments to wetlands that are hydrologically isolated from the Mississippi River due to the construction of levees. In this dissertation, I examined effects of the second largest freshwater diversion in Louisiana, the Caernarvon Freshwater Diversion (CFD), on estuarine nekton in Breton Sound. Before focusing on Breton Sound, I examined the status of nekton communities in the northern Gulf of Mexico (GOM), and Louisiana wetlands in particular, using the mean trophic level index (MTLI). I demonstrated that commercial targeting caused the previously reported low and declining MTLI from the GOM. …

Effect Of Resistant Starch On Microbial Content Of The Intestinal Tract, Reshani Nisansala Senevirathne

LSU Doctoral Dissertations

Resistant starch (RS) increases beneficial gastro-intestinal bacterial populations simultaneously increasing short-chain fatty acids (SCFA) such as butyrate. Butyrate acts as energy source for epithelial cells of colonic mucosa which stimulates intestinal cell proliferation and has been implicated as important in reducing obesity, diabetes and cancer. The Lactobacillus spp, Bifidobacterium spp, Bacteroides thetaiotaomicron, Clostridium cluster IV and Clostridium cluster XIV are mainly involved in the production of butyrate by fermenting RS in the large intestine. With age there is a decline in these gut bacteria, but in the present study RS and diet restriction both enhanced the bacteria involved in butyrate …

Probing The Finance Gap Theory: Does Gender Affect Campaign Contributions?, Rhonda Wrzenski

LSU Doctoral Dissertations

In our field, scholarly literature contends that women win a similar percentage of the vote and raise a similar amount of money as their male opponents when they campaign (Newman 1994; Burrell 1994; Seltzer et al. 1997; Dolan 2006; Darcy et al. 1994; Wilhite and Theilmann 1986; Burrell 1985; Uhlaner and Schlozman 1986; Welch et al. 1985). However, women are still underrepresented at all levels of government and this may be a function of biases in the campaign finance network that have gone largely undetected. Differences may be more readily apparent at the sub-national level and among particular classes of …

Optimized Extraction Of Soluble Defatted Rice Bran Fiber And Its Application For Microencapsulation Of Fish Oil, Yuting Wan

LSU Master's Theses

Defatted rice bran (DRB) is a byproduct of rice milling and rice bran oil extraction. Soluble rice bran fiber (SRBF) extracted from defatted rice bran is known for its antioxidant activity and hypocholesterolemic effects in human, while purified menhaden oil (PMO) is a good source of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). The goal of the study was to estimate optimum extraction conditions to extract SRBF from DRB, develop a cost effective method to purify SRBF and produce microencapsulated PMO with SRBF. The response surface methodology showed that an estimated optimum yield of SRBF (7.89%) could be extracted from …

Dietary Resistant Starch Improved Maternal Glycemic Control In Goto-Kakizaki Rat, Li Shen

LSU Doctoral Dissertations

Treatment for maternal hyperglycemia is limited on account of safety concerns for fetus. Our previous work has shown that inclusion of resistant starch in the diet decreases body fat accumulation in rodents, increases GLP-1 at both the gene expression level and plasma levels, and improves glucose tolerance in STZ-induced diabetic mice. However, studies concerning dietary resistant starch and maternal hyperglycemia are scarce. In this project, we examined the effects of dietary resistant starch in pregnant Goto-Kakizaki (GK) rats to improve glycemic control. Animal experiments were conducted to test the hypotheses. In study 1, the female GK rats were randomly grouped …

Interaction Of Tumor Necrosis Factor-Alpha And The Renin Angiotensin System In The Pathogenesis Of Hypertension, Srinivas Sriramula

LSU Doctoral Dissertations

Hypertension is a major predisposing factor for the development of cardiovascular and renal diseases. The renin-angiotensin system (RAS) plays a pivotal role in the pathogenesis of cardiovascular diseases such as hypertension, myocardial infarction, heart failure, and stroke. Angiotensin II (Ang II), the effector peptide of the RAS, activates a wide spectrum of signaling responses via the Ang II type-I receptor that mediate its physiological control of blood pressure, thirst and sodium balance. For the past two decades, increasing evidence has demonstrated that the circulatory RAS and local/tissue RAS components (heart and brain) may contribute to the development of hypertensive response. …

Maladies Of Modernity: Scientism In Politics, David Nathan Whitney

LSU Doctoral Dissertations

This dissertation explores the complex relationship between science and politics. More specifically, it focuses on the problem of scientism. Features of scientism include the dogmatic faith in the methods of the natural sciences (and the accompanying assumption that those methods can be successfully imported into the social sciences), a materialistic worldview, the rejection of the bios theoretikos, the prohibition of philosophical questions, and an emphasis on immanent fulfillment through the power of science. I seek to demonstrate that scientism is intellectually impoverishing and politically dangerous. Given the lack of full-length studies on the subject, I trace the development of scientism …

Texasweed (Caperonia Palustris (L.) St. Hil.) Interference And Management In Drill-Seeded Rice, Rakesh K. Godara

LSU Doctoral Dissertations

Field research was conducted from 2006 to 2009 to study Texasweed [Caperonia palustris (L.) St. Hil.] interference and management in Cocodrie rice. Texasweed interference at 10 plants/m2 caused 24 to 31% rice yield reduction. The maximum possible yield loss was estimated to be 81%. Rice yield reduction was primarily due to a reduction in culms/m2 and filled grains per panicle. For maximum yield, Texasweed must be removed by two weeks after emergence and managed until permanent flood establishment. Shade had no effect on Texasweed emergence but significantly reduced growth and reproduction. At 100 days after emergence, 50, 70, and 90% …