Digital Commons Network^™

Insights Into Abscisic Acid And Phosphatidic Acid Signal Transduction Dynamics In Arabidopsis Thaliana, Ruth Wanjiru Ndathe

LSU Doctoral Dissertations

Abscisic acid (ABA) is a plant hormone that triggers a signaling transduction in response to drought, salinity, and cold. The core components in the ABA signaling pathway that lead to downstream ABA responses have been identified.

Phosphatidic acid (PA) is a membrane phospholipid that is involved in the signaling pathway for ABA-induced stomatal closure. We tested whether PA is involved in the signaling pathway for ABA-induced gene expression as well, using model plant Arabidopsis thaliana. We selected the RD29A gene which is a canonical drought gene that is induced by ABA. We found that PA is not involved in …

Genetic Structuring And Community Assembly In Neotropical Birds, Oscar Johnson

LSU Doctoral Dissertations

The factors that influence how species form is of fundamental interest to biology. Under the framework of the speciation cycle, the process begins by population subdivision creating structured populations that then diverge until two new species form. Following speciation, however, continued divergence is necessary for these new species to be able to coexist without adverse interactions, known as the transition to secondary sympatry. At this point the speciation cycle is complete and the process can begin anew. Many of the factors influencing these processes remain poorly understood. Here, I explore the factors that influence speciation and community assembly in Neotropical …

Temperature Affects The Time Required To Discern The Relationship Between Primary Production And Export Production In The Ocean, Edward Laws, Kanchan Maiti

Faculty Publications

No abstract provided.

Temperature Affects The Time Required To Discern The Relationship Between Primary Production And Export Production In The Ocean, Edward Laws, Kanchan Maiti

Faculty Publications

No abstract provided.

Consumers' Perception Of Food Containing Insect Protein Affected By Serving Condition And Questionnaire Protocol, Yupeng Gao

LSU Master's Theses

Edible insects are a new sustainable protein source compared to meat. Western consumers show aversion to the prospect of insects as food due to unfamiliarity, food neophobia, and negative emotions. This dissertation research explored consumers’ emotional perception of insect-based food products presented under different testing conditions and examined the influence of other people’s emotional expressions towards consumers’ purchase decisions. To identify and rate emotion intensities, tests were conducted using three presenting formats including text, image, and an actual product; all of which were applied for both cookies containing insects and without insects. In addition to these presenting formats, news of …

High Precision Tissue Sampling For Mass Spectrometry, Chao Dong

LSU Doctoral Dissertations

A high precision infrared laser ablation sampling system was developed for localized tissue proteomics. A Schwarzschild reflective objective with 10 cm working distance and 0.3 numerical aperture was used to focus a mid-IR laser at 3 µm wavelength to a beam diameter of approximately 6 µm. A laser ablation spot size of 10 µm was achieved on thin ink films and tissue sections. The sampling system was used to ablate and capture rat brain tissue sections in transmission geometry. Captured tissue protein was extracted, digested, and analyzed with offline liquid chromatography tandem mass spectrometry (LC-MS/MS) for peptide and protein identification. …

Genome-Wide Analysis Of Peg3 Downstream Genes, Subash Ghimire

LSU Doctoral Dissertations

Paternally expressed gene 3 (Peg3) is an imprinted gene encoding a DNA-binding protein that is a predicted transcriptional repressor. The two studies presented here demonstrate the repressive functions of PEG3 in regulating different cellular processes and pathways, which were observed by analyzing the ChIP-seq results systematically using both bioinformatics tools and manual inspection of significant peaks. In the first part of the work, genome-wide features of ChIP-seq peaks were characterized and the potential de novo binding motif was reevaluated. Results suggested the transcriptional repressor role of PEG3 by binding to the conserved sequence motif of the target genes. …

Ontogeny And Resource Use Of Invasive Lionfish, Pterois Volitans, In Shallow Water Habitats Surrounding Summerland Key, Emily Shallow

LSU Master's Theses

The invasive red lionfish, Pterois volitans, has been shown to considerably reduce reef biomass via predation and competition in non-native ranges. Primary maintenance of lionfish is through human removal (e.g., culling), which does not sufficiently control the population due to deep-water spawning, recolonization of recently culled reefs, and ontogenetic migrations to mesophotic waters. Here, I investigated the diets and primary energy pathways during ontogeny in lionfish from the Florida Keys using stomach contents and stable isotope (δ¹³C and δ¹⁵N) analysis to better understand their role in the trophic food web. Additionally, isotopic overlap between lionfish …

An Erp Study Of The Effects Of Iconic And Nonsense Gestures On Memory Formation, Brianna E. Cairney

LSU Master's Theses

Co-speech gesture is an important part of human communication and aids in comprehension, learning, and memory. The addition of iconic gestures to speech has been shown in prior work to enhance memory for the speech. However, it remains unclear as to whether this benefit requires gestures to be meaningful, or, conversely, if any attentionally-engaging gesture will enhance memory. In the current study, we tested two theories to explain the mnemonic benefits of co-speech gesture: Dual Coding Theory, which attributes these benefits to multimodal encoding and enhanced imageability, and Attentional Highlighting Theory, which posits that gestures draw more attention to concurrent …

Protein Appetite At The Interface Between Nutrient Sensing And Physiological Homeostasis, Md Shahjalal Khan, Redin A. Spann, Heike Münzberg, Sangho Yu, Vance L. Albaugh, Yanlin He, Hans Rudolf Berthoud, Christopher D. Morrison

Division of Basic Science Faculty Publications

Feeding behavior is guided by multiple competing physiological needs, as animals must sense their internal nutritional state and then identify and consume foods that meet nutritional needs. Dietary protein intake is necessary to provide essential amino acids and represents a specific, distinct nutritional need. Consistent with this importance, there is a relatively strong body of literature indicating that protein intake is defended, such that animals sense the restriction of protein and adaptively alter feeding behavior to increase protein intake. Here, we argue that this matching of food consumption with physiological need requires at least two concurrent mechanisms: the first being …

Tau-Atrophy Variability Reveals Phenotypic Heterogeneity In Alzheimer's Disease, Sandhitsu R. Das, Xueying Lyu, Michael Tran Duong, Long Xie, Lauren Mccollum, Robin De Flores, Michael Dicalogero, David J. Irwin, Bradford C. Dickerson, Ilya M. Nasrallah, Paul A. Yushkevich, David A. Wolk, Michael Weiner, Paul Aisen, Ronald Petersen, Clifford R. Jack, William Jagust, John Q. Trojanowki, Arthur W. Toga, Laurel Beckett, Robert C. Green, Andrew J. Saykin, John Morris, Leslie M. Shaw, Enchi Liu, Tom Montine, Ronald G. Thomas, Michael Donohue, Michael Donohue, Sarah Walter, Devon Gessert, Tamie Sather, Gus Jiminez, Danielle Harvey

Clinical Research Faculty Publications

Objective: Tau neurofibrillary tangles (T) are the primary driver of downstream neurodegeneration (N) and subsequent cognitive impairment in Alzheimer's disease (AD). However, there is substantial variability in the T-N relationship – manifested in higher or lower atrophy than expected for level of tau in a given brain region. The goal of this study was to determine if region-based quantitation of this variability allows for identification of underlying modulatory factors, including polypathology. Methods: Cortical thickness (N) and 18F-Flortaucipir SUVR (T) were computed in 104 gray matter regions from a cohort of cognitively-impaired, amyloid-positive (A+) individuals. Region-specific residuals from a robust linear …

A Reference Genome For The Nectar-Robbing Black-Throated Flowerpiercer (Diglossa Brunneiventris), A E. Hiller, R T. Brumfield, B C. Faircloth

Faculty Publications

Black-throated Flowerpiercers (Diglossa brunneiventris) are one species representing a phenotypically specialized group of tanagers (Thraupidae) that have hooked bills which allow them to feed by stealing nectar from the base of flowers. Members of the genus are widely distributed in montane regions from Mexico to northern Argentina, and previous studies of Diglossa have focused on their systematics, phylogenetics, and interesting natural history. Despite numerous studies of species within the genus, no genome assembly exists to represent these nectivorous tanagers. We described the assembly of a genome sequence representing a museum-vouchered, wild, female D. brunneiventris collected in Peru. By combining Pacific …

Genetic Responses And Aflatoxin Inhibition During Co-Culture Of Aflatoxigenic And Non-Aflatoxigenic Aspergillus Flavus, Rebecca R. Sweany, Brian M. Mack, Geromy G. Moore, Matthew K. Gilbert, Jeffrey W. Cary, Matthew D. Lebar, Kanniah Rajasekaran, Kenneth E. Damann

Faculty Publications

Aflatoxin is a carcinogenic mycotoxin produced by Aspergillus flavus. Non-aflatoxigenic (Non-tox) A. flavus isolates are deployed in corn fields as biocontrol because they substantially reduce aflatoxin contamination via direct replacement and additionally via direct contact or touch with toxigenic (Tox) isolates and secretion of inhibitory/degradative chemicals. To understand touch inhibition, HPLC analysis and RNA sequencing examined aflatoxin production and gene expression of Non-tox isolate 17 and Tox isolate 53 mono-cultures and during their interaction in co-culture. Aflatoxin production was reduced by 99.7% in 72 h co-cultures. Fewer than expected unique reads were assigned to Tox 53 during co-culture, indicating its …

Analysis Of The Photodegradation Of The Pro-Herbicide Benzobicyclon Hydrolysate Using A Seawater Gradient And Constituent Ions, Margaret A. Knight

Honors Capstones

No abstract provided.

Use Of Racial And Ethnic Categories In Medical Testing And Diagnosis: Primum Non Nocere, Jay S. Kaufman, Joanna Merckx, Richard S. Cooper

Faculty Publications

BACKGROUND: Use of race and ethnicity is common in medical tests and procedures, even though these categories are defined by sociological, historical, and political processes, and vary considerably in their definitions over time and place. Because all societies organize themselves around these constructs in some way, they are undeniable facets of the human experience, with myriad health consequences. In the biomedical literature, they are also commonly interpreted as representing biological heterogeneity that is relevant for health and disease. CONTENT: We review the use of race and ethnicity in medical practice, especially in the USA, and provide 2 specific examples to …

Physical Activity And Fat-Free Mass During Growth And In Later Life, Klaas R. Westerterp, Yosuke Yamada, Hiroyuki Sagayama, Philip N. Ainslie, Lene F. Andersen, Liam J. Anderson, Lenore Arab, Issaad Baddou, Kweku Bedu-Addo, Ellen E. Blaak, Stephane Blanc, Alberto G. Bonomi, Carlijn V.C. Bouten, Pascal Bovet, Maciej S. Buchowski, Nancy F. Butte, Stefan G.J.A. Camps, Graeme L. Close, Jamie A. Cooper, Sai K. Das, Richard Cooper, Lara R. Dugas, Ulf Ekelund, Sonja Entringer, Terrence Forrester, Barry W. Fudge, Annelies H. Goris, Michael Gurven, Catherine Hambly, Asmaa E. El Hamdouchi, Marije B. Hoos, Sumei Hu, Noorjehan Joonas

Faculty Publications

Background: Physical activity may be a way to increase and maintain fat-free mass (FFM) in later life, similar to the prevention of fractures by increasing peak bone mass. Objectives: A study is presented of the association between FFM and physical activity in relation to age. Methods: In a cross-sectional study, FFM was analyzed in relation to physical activity in a large participant group as compiled in the International Atomic Energy Agency Doubly Labeled Water database. The database included 2000 participants, age 3-96 y, with measurements of total energy expenditure (TEE) and resting energy expenditure (REE) to allow calculation of physical …

Resistance To Aflatoxin Accumulation In Maize Mediated By Host-Induced Silencing Of The Aspergillus Flavus Alkaline Protease (Alk) Gene, Olanike Omolehin, Yenjit Raruang, Dongfang Hu, Zhu Qiang Han, Qijian Wei, Kan Wang, Kanniah Rajasekaran, Jeffrey W. Cary, Zhi Yuan Chen

Faculty Publications

Aspergillus flavus is a fungal pathogen that infects maize and produces aflatoxins. Host-Induced Gene Silencing (HIGS) has been shown to reduce host infection by various fungal pathogens. Here, the A. flavus alkaline protease (alk) gene was targeted for silencing through HIGS. An RNAi vector carrying a portion of the alk gene was incorporated into the B104 maize genome. Four out of eight transformation events containing the alk gene, Alk-3, Alk-4, Alk-7 and Alk-9, were self-pollinated to T4/T6 generations. At T3, the Alk-transgenic lines showed up to 87% reduction in aflatoxin accumulation under laboratory conditions. T4 transgenic Alk-3 and Alk-7 lines, …

Effects Of Cover Crops On Beetle Assemblages In Tea Plantations, Li Lin Chen, Gabor Pozsgai, Xiang Yu Li, Lei Li, Gadi V.P. Reddy, Min Sheng You

Faculty Publications

Coleoptera are visible members of food webs in tea plantations, with high species richness and abundance. Many tea pests, as well as natural enemies, are members of this order, so a knowledge of how groundcovers affect beetles can aid pest management. We collected beetles in a replicated field experiment in the Wuyi Mountains, Fujian Province, China. Tea was intercropped with Paspalum notatum or Chamaecrista rotundifolia, or rows were cleared to bare ground, or in the control they were left unmanaged to allow weeds to grow naturally. Sampling, done by sweep netting and vegetation beating, was conducted monthly, between May 2006 …

Juul 3% Menthol Aerosol Extracts Exhibit Toxicity And Alter Gene Expression Of Murine Macrophages, Samantha Jindia

Honors Capstones

No abstract provided.

Analysis Of Young, Lineage Specific Alu Elements In Cebus, Catherine Rockwell

Honors Capstones

No abstract provided.

Next-Generation Ensemble Projections Reveal Higher Climate Risks For Marine Ecosystems, Derek P. Tittensor, Camilla Novaglio, Cheryl S. Harrison, Ryan F. Heneghan, Nicolas Barrier, Daniele Bianchi, Laurent Bopp, Andrea Bryndum-Buchholz, Gregory L. Britten, Matthias Büchner, William W. L. Cheung, Villy Christensen, Marta Coll, John P. Dunne, Tyler D. Eddy, Jason D. Everett, Jose A. Fernandes-Salvador, Elizabeth A. Fulton, Eric D. Galbraith, Didier Gascuel, Jerome Guiet, Jasmin G. John, Jason S. Link, Heike K. Lotze, Olivier Maury, Kelly Ortega-Cisneros, Juliano Palacios-Abrantes, Colleen M. Petrik, Hubert Du Pontavice, Jonathan Rault, Anthony J. Richardson, Lynne Shannon, Yunne-Jai Shin

Faculty Publications

AbstractProjections of climate change impacts on marine ecosystems have revealed long-term declines in global marine animal biomass and unevenly distributed impacts on fisheries. Here we apply an enhanced suite of global marine ecosystem models from the Fisheries and Marine Ecosystem Model Intercomparison Project (Fish-MIP), forced by new-generation Earth system model outputs from Phase 6 of the Coupled Model Intercomparison Project (CMIP6), to provide insights into how projected climate change will affect future ocean ecosystems. Compared with the previous generation CMIP5-forced Fish-MIP ensemble, the new ensemble ecosystem simulations show a greater decline in mean global ocean animal biomass under both strong-mitigation …

Development Of An Open Hardware 3-D Printed Conveyor Device For Continuous Cryopreservation Of Non-Batched Samples, Yue Liu, Melissa Eskridge, Amy Guitreau, Jacob Beckham, Megan Chesnut, Leticia Torres, Terrence R. Tiersch, William Todd Monroe

Faculty Publications

A great challenge among communities participating in germplasm repository development is to obtain suitable cryopreservation equipment and devices. Commercial programmable freezers are costly and thus unaffordable to many users. Self-made devices have substantial variability, resulting in few opportunities for standardization across communities. The development of open hardware with the increasing accessibility of three-dimensional (3-D) printing offers rapid prototyping and easy fabrication of devices by users around the world at low cost. The present study explored the feasibility of developing operational prototypes of 3-D printed motorized cryopreservation devices for continuous freezing of non-batched samples. A controlled cooling conveyor device (CCCD) was …

Rickettsia Conorii Survival In Thp-1 Macrophages Involves Host Lipid Droplet Alterations And Active Rickettsial Protein Production, Paige E. Allen, Robert C. Noland, Juan J. Martinez

Division of Basic Science Faculty Publications

Rickettsia conorii is a Gram-negative, cytosolic intracellular bacterium that has classically been investigated in terms of endothelial cell infection. However, R. conorii and other human pathogenic Rickettsia species have evolved mechanisms to grow in various cell types, including macrophages, during mammalian infection. During infection of these phagocytes, R. conorii shifts the host cell's overall metabolism towards an anti-inflammatory M2 response, metabolically defined by an increase in host lipid metabolism and oxidative phosphorylation. Lipid metabolism has more recently been identified as a key regulator of host homeostasis through modulation of immune signalling and metabolism. Intracellular pathogens have adapted mechanisms of hijacking …

Leptin Treatment Prevents Impaired Hypoglycemic Counterregulation Induced By Exposure To Severe Caloric Restriction Or Exposure To Recurrent Hypoglycemia, Marina A. Duvall, Carolyn E. Coulter, Jasmin L. Gosey, Matthew J. Herrera, Cristal M. Hill, Rajvi R. Jariwala, Lauren E. Maisano, Laura A. Moldovan, Christopher D. Morrison, Ngozi V. Nwabueze, Hunter X. Sikaffy, David H. Mcdougal

Division of Basic Science Faculty Publications

Hypoglycemia-associated autonomic failure (HAAF) is a maladaptive failure in glucose counterregulation in persons with diabetes (PWD) that is caused by recurrent exposure to hypoglycemia. The adipokine leptin is known to regulate glucose homeostasis, and leptin levels fall following exposure to recurrent hypoglycemia. Yet, little is known regarding how reduced leptin levels influence glucose counterregulation, or if low leptin levels are involved in the development of HAAF. The purpose of this study was to determine the effect of hypoleptinemia on the neuroendocrine responses to hypoglycemia. We utilized two separate experimental paradigms known to induce a hypoleptinemic state: 60% caloric restriction (CR) …

Factors Associated With Being Overweight And Obesity In People Living With Human Immunodeficiency Virus On Antiretroviral Therapy: Socioclinical, Inflammation, And Metabolic Markers, Jeanne Goupil De Bouillé, Corinne Vigouroux, Lorraine Plessis, Mathilde Ghislain, Jean Paul Teglas, Faroudy Boufassa, Cécile Goujard, Dorothée Vignes, Olivier Bouchaud, Dominique Salmon, Laurence Meyer, Sophie Abgrall, O. B., O. B., F. B., F. B., Robert Carlier, Rosemary Dray-Spira, M. G., C. G., C. G., C. G., L. P., L. P., Lucie Marchand, L. M., Ventzislava Petrov-Sanchez, Christine Rouzioux, Rémonie Seng, Rémonie Seng, Rodolphe Thiebaut, Jean Paul Viard, Jean Paul Viard, S. A.

Division of Basic Science Faculty Publications

Background: We investigated the association between socioclinical, inflammatory, and metabolic markers and weight gain in people with human immunodeficiency virus (HIV) on combination antiretroviral therapy (cART). Methods: Individuals from the COPANA cohort of normal weight (body mass index [BMI], 18.5-24.9 [calculated as weight in kilograms divided by height in meters squared) at cART initiation who achieved virological suppression (viral load, < 50 copies/mL) and maintained it through 36 months of treatment were selected. Clinical, immunovirological, and socioeconomic data and inflammation (high-sensitivity C-reactive protein, CXCL10, CXCL8, interleukin 6, soluble tumor necrosis factor receptors 1 and 2, soluble CD14, and soluble CD16) and serum metabolic (glucose, insulin, lipid profile, adiponectin, and leptin) markers were assessed. Factors associated with becoming overweight (BMI, 25-29.9) or obese (BMI, ≥30) at 36 months were assessed using multivariate logistic regression models. Results: After 36 months of cART, 32 of 158 people with HIV (20%) became overweight or obese (21% female; 65% born in France and 23% born in sub-Saharan Africa; median BMI at cART initiation, 22 [interquartile range, 21-23]). After adjustment, higher BMI, originating from sub-Saharan Africa, living in a couple, and higher soluble tumor necrosis factor receptor 2 and lower adiponectin concentrations at cART initiation were associated with becoming overweight or obese. Conclusion: Weight gain on cART is multifactorial. Special attention should be given to migrants from sub-Saharan Africa. Monocyte activation and adipocyte dysfunction at cART initiation affect weight regulation.

Grow Fast, Die Young: Does Compensatory Growth Reduce Survival Of Juvenile Blacktip Sharks (Carcharhinus Limbatus) In The Western Gulf Of Mexico?, Philip Matich, Jeffrey D. Plumlee, Mark Fisher

Faculty Publications

Effective conservation and management necessitate an understanding of the ecological mechanisms that shape species life histories in order to predict how variability in natural and anthropogenic impacts will alter growth rates, recruitment, and survival. Among these mechanisms, the interaction between parturition timing and prey availability frequently influences offspring success, particularly when postnatal care is absent. Here, we assess how parturition timing and nursery conditions, including prey abundance and environmental conditions, influence the growth and potential survival of blacktip sharks (Carcharhinus limbatus) in western Gulf of Mexico (GOM) estuaries over their first year. Catch data from long-term gillnet monitoring allowed for …

Survival Of Western Gulf Coast Mottled Ducks (Anas Fulvigula) In The Path Of A Category 4 Hurricane, Kevin M. Ringelman, Elizabeth S. Bonczek, Joseph R. Marty, Ashley R. Booth, Alexandre L. Dopkin

Faculty Publications

Tropical cyclones are the most powerful storms on earth, causing catastrophic damage to human lives and infrastructure. Hurricanes also cause wildlife mortality when they make landfall, but the severity of these effects is difficult to quantify because data collection is either logistically impossible or deprioritized in the wake of human tragedy. On August 27, 2020, Hurricane Laura made landfall in southwestern Louisiana with maximum sustained winds of 241 kph (150 mph), making it one of the most powerful storms to strike the mainland United States. Hurricane Laura passed directly over the core breeding range of the western Gulf Coast population …

Morphological Consequences Of Climate Change For Resident Birds In Intact Amazonian Rainforest, Vitek Jirinec, Ryan C. Burner, Bruna R. Amaral, Richard O. Bierregaard, Gilberto Fernández-Arellano, Angélica Hernández-Palma, Erik I. Johnson, Thomas E. Lovejoy, Luke L. Powell, Cameron L. Rutt, Jared D. Wolfe, Philip C. Stouffer

Faculty Publications

Warming from climate change is expected to reduce body size of endotherms, but studies from temperate systems have produced equivocal results. Over four decades, we collected morphometric data on a nonmigratory understory bird community within Amazonian primary rainforest that is experiencing increasingly extreme climate. All 77 species showed lower mean mass since the early 1980s-nearly half with 95% confidence. A third of species concomitantly increased wing length, driving a decrease in mass:wing ratio for 69% of species. Seasonal precipitation patterns were generally better than temperature at explaining morphological variation. Short-term climatic conditions affected all metrics, but time trends in wing …

Erratum: Range-Wide Population Genetic Analysis Of Seaside Sparrows (Ammospiza Maritima) Supports At Least Five Distinct Population Segments That Do Not Align With Current Subspecies Descriptions (The Condor Doi: 10.1093/Ornithapp/Duab019), Kathryn E.C. Davis, Amie E. Settlecowski, Mackenzie R. Roeder, Carolyn Enloe, Thomas Virzi, Margaret E. Hunter, Stefan Woltmann, Sabrina S. Taylor

Faculty Publications

In the originally published version of this manuscript, the author Stefan Woltmann was erroneously given affiliation 3, Florida Fish and Wildlife Conservation Commission, Lovett E. Williams Jr. Wildlife Research Lab, Gainesville, Florida, USA. Dr. Woltmann s correct information is affiliation 2, Department of Biology and Center of Excellence for Field Biology, Austin Peay State University, Clarksville, Tennessee, USA, and this has been updated in the original article. The publisher regrets this error.

Association Between The Fto Rs9939609 Single Nucleotide Polymorphism And Dietary Adherence During A 2-Year Caloric Restriction Intervention: Exploratory Analyses From Calerie™ Phase 2, James L. Dorling, Daniel W. Belsky, Susan B. Racette, Sai Krupa Das, Eric Ravussin, Leanne M. Redman, Christoph Höchsmann, Kim M. Huffman, William E. Kraus, Michael S. Kobor, Julia L. Macisaac, David T.S. Lin, David L. Corcoran, Corby K. Martin

Clinical Research Faculty Publications

Caloric restriction (CR) improves markers of aging in humans; but it is not known if the fat mass and obesity-associated gene (FTO) rs9939609 single nucleotide polymorphism (SNP), which is associated with appetite and energy intake, influences adherence to prolonged CR. Utilizing data from the two-year Comprehensive Assessment of Long-term Effects of Reducing Intake of Energy (CALERIE™) phase 2 randomized controlled trial, we tested whether the FTO rs9939609 SNP was associated with adherence to CR in healthy adults without obesity. As secondary aims, we assessed whether the FTO rs9939609 SNP was associated with changes in body composition, biomarkers of aging, and …