Digital Commons Network^™

Uterine Lumen Fluid Is Metabolically Semi-Autonomous, Constantine A. Simintiras, Jessica N. Drum, Hongyu Liu, M. Sofia Ortega, Thomas E. Spencer

Faculty Publications

Uterine lumen fluid (ULF) is central to successful pregnancy establishment and maintenance, and impacts offspring wellbeing into adulthood. The current dogma is that ULF composition is primarily governed by endometrial glandular epithelial cell secretions and influenced by progesterone. To investigate the hypothesis that ULF is metabolically semi-autonomous, ULF was obtained from cyclic heifers, and aliquots incubated for various durations prior to analysis by untargeted semi-quantitative metabolomic profiling. Metabolite flux was observed in these ULF isolates, supporting the idea that the biochemical makeup of ULF is semi-autonomously dynamic due to enzyme activities. Subsequent integrative analyses of these, and existing, data predict …

Characterization Of A Stress-Enhanced Promoter From The Grass Halophyte, Spartina Alterniflora L., Sonali Sengupta, Necla Pehlivan, Venkata Mangu, Kanniah Rajasekaran, Niranjan Baisakh

Faculty Publications

Stress-inducible promoters are vital for the desirable expression of genes, especially transcription factors, which could otherwise compromise growth and development when constitutively overexpressed in plants. Here, we report on the characterization of the promoter region of a stress-responsive gene SaAsr1 from monocot halophyte cordgrass (Spartina alterniflora). Several cis-acting elements, such as ABRE (ABA-responsive element), DRE-CRT (dehydration responsive-element/C-Repeat), LTRE (low temperature-responsive element), ERE (ethylene-responsive element), LRE (light-responsive element), etc. contributed at varying degrees to salt-, drought- and ABA-enhanced expression of gusA reporter gene in Arabidopsis thaliana under the full-length promoter, pAsr11875 and its deletion derivatives with an assortment of cis-regulatory motifs. …

Health Physics Workforce In The United States, Michael A. Noska, Caridad Borrás, E. Vincent Holahan, Shaheen A. Dewji, Thomas E. Johnson, Jerry W. Hiatt, Wayne D. Newhauser, John W. Poston, Nolan Hertel

Faculty Publications

No abstract provided.

Medical Physics Workforce In The United States, Wayne D. Newhauser, Dustin A. Gress, Michael D. Mills, David W. Jordan, Steven G. Sutlief, Melissa C. Martin, Edward Jackson

Faculty Publications

No abstract provided.

Greater Evolutionary Divergence Of Thermal Limits Within Marine Than Terrestrial Species, Matthew Sasaki, Jordanna M. Barley, Sarah Gignoux-Wolfsohn, Cynthia G. Hays, Morgan W. Kelly, Alysha B. Putnam, Seema N. Sheth, Andrew R. Villeneuve, Brian S. Cheng

Faculty Publications

There is considerable uncertainty regarding which ecosystems are most vulnerable to warming. Current understanding of organismal sensitivity is largely centred on species-level assessments that do not consider variation across populations. Here we used meta-analysis to quantify upper thermal tolerance variation across 305 populations from 61 terrestrial, freshwater, marine and intertidal taxa. We found strong differentiation in heat tolerance across populations in marine and intertidal taxa but not terrestrial or freshwater taxa. This is counter to the expectation that increased connectivity in the ocean should reduce intraspecific variation. Such adaptive differentiation in the ocean suggests there may be standing genetic variation …

Interactions Of Cdkal1 Rs7747752 Polymorphism And Serum Levels Of L-Carnitine And Choline Are Related To Increased Risk Of Gestational Diabetes Mellitus, Hui Wang, Jing Li, Jinnan Liu, Junhong Leng, Weiqin Li, Zhijie Yu, Claudia H.T. Tam, Gang Hu, Ronald C.W. Ma, Zhongze Fang, Ying Wang, Xilin Yang

Population and Public Health Science Faculty Publications

Background: Interactions between genetic, metabolic, and environmental factors lead to gestational diabetes mellitus (GDM). We aimed to examine interactive effects of cyclin-dependent kinase 5 regulatory subunit-associated protein1-like 1(CDKAL1) rs7747752 polymorphism with low serum levels of L-carnitine, choline, and betaine for GDM. Methods: A nested case-control study of 207 GDM women and their one-to-one, age-matched controls was organized from a prospective cohort of pregnant women in Tianjin, China. Conditional logistic regressions were used to test associations between CDKAL1 rs7747752 and serum levels of L-carnitine, choline, and betaine, and the risk of GDM. Additive interactions were performed to examine interactive effects of …

Evaluating The Viscoelastic Properties Of Alligator Skin Gelatin, Alligel, Compared To Mammalian And Fish Gelatins, Sarah E. Jimenez

Honors Capstones

No abstract provided.

Variation In Human Water Turnover Associated With Environmental And Lifestyle Factors, Yosuke Yamada, Xueying Zhang, Mary E.T. Henderson, Hiroyuki Sagayama, Herman Pontzer, Daiki Watanabe, Tsukasa Yoshida, Misaka Kimura, Philip N. Ainslie, Lene F. Andersen, Liam J. Anderson, Lenore Arab, Issad Baddou, Kweku Bedu-Addo, Ellen E. Blaak, Stephane Blanc, Alberto G. Bonomi, Carlijn V.C. Bouten, Pascal Bovet, Maciej S. Buchowski, Nancy F. Butte, Stefan G. Camps, Graeme L. Close, Jamie A. Cooper, Richard Cooper, Sai Krupa Das, Lara R. Dugas, Simon Eaton, Ulf Ekelund, Sonja Entringer, Terrence Forrester, Barry W. Fudge, Annelies H. Goris

Faculty Publications

Water is essential for survival, but one in three individuals worldwide (2.2 billion people) lacks access to safe drinking water. Water intake requirements largely reflect water turnover (WT), the water used by the body each day. We investigated the determinants of human WT in 5604 people from the ages of 8 days to 96 years from 23 countries using isotope-tracking (2H) methods. Age, body size, and composition were significantly associated with WT, as were physical activity, athletic status, pregnancy, socioeconomic status, and environmental characteristics (latitude, altitude, air temperature, and humidity). People who lived in countries with a low human development …

Estimation Of Economic Risk From Coastal Natural Hazards In Louisiana, Rubayet Bin Mostafiz

LSU Doctoral Dissertations

Louisiana, U.S.A., is among the most vulnerable areas globally to coastal natural hazards, with risk vulnerability likely increasing. The risks associated with non-tropical-cyclone hazards in Louisiana’s coastal zone have been understudied. This research enhances present and future (i.e., 2050) Louisiana risk assessment using locally-weighted, model-based hazard frequency/intensity and population projections.

Results suggest that property risks associated with extreme cold temperature and tornado are and will remain costlier than those for hail and lightning. Property risks of extreme cold temperature and hail are projected to decrease with the expected warming temperatures, with those of all four of these hazards peaking in …

The Effects Of Tillage And Cover Crops On Crop Yield And Soil Properties In Wheat Double-Cropping Systems, Peters E. Egbedi

LSU Doctoral Dissertations

This three-year study was conducted on a commerce silt loam soil at the Northeast Research and Experiment Station near St. Joseph, Louisiana to evaluate the following objectives: 1) evaluate crop yield response to tillage, winter, and summer cover crops in wheat double-cropping systems. 2) evaluate the effects of tillage, winter and summer cover crops on soil properties under different cropping systems. 3) quantify the economic benefits of double-cropping, cover cropping and monoculture systems under conventional and no tillage practices. A winter cover crop (WCC) mix (winter wheat (Triticum aestivum and Austrian winter pea (Pisum sativum) and summer …

Studies Of Roseau Cane Dieback In The Lower Mississippi River Delta Based On Remote Sensing Data Including Landsat, Worldview, And Drone, Nan Shang

LSU Doctoral Dissertations

This research focused on the Roseau cane (Phragmites australis) dieback assessment in the lower MRD of Louisiana and introduced a comprehensive and systematic multi-source remote sensing method for assessing wetland and Roseau cane dieback and habitat dynamics analysis from three scale levels from large-scale to small scale.

Large-scale historical vegetation/land change analyses were conducted in the lower MRD based on Landsat in the past two decades (2001 - 2021). A strong increasing trend of vegetation was found since 2005. Around 51 km² ofdieback area was detected which accounts for 11% of the overall vegetation coverage. This research …

Numerical Investigation Of Heat Generation And Accumulation Contributing To Elevated Temperature In Msw Landfills, Alborz Fathinezhad

LSU Doctoral Dissertations

Landfills are complex geostructures which contains organic and inorganic municipal, and in some cases industrial, wastes and are expected to remain operational for long times. Due to the complex nature of physical, chemical, biological, and thermal reactions that carry on within the depths of a landfill, unexpected incidents such as elevated temperatures could become inevitable. While uncommon to happen, elevated temperatures cause health and environmental issues such as odors, rapid settlements, slope instabilities. In addition, elevated temperatures can negatively impact engineered components in composite bottom liners, cover systems, leachate collection, and gas extraction and recovery systems.

Air intrusion into municipal …

Spatiotemporal Transcriptome Diversity And Responses To Salinity Tolerance In The Extremophyte Schrenkiella Parvula, Chathura Wijesinghege

LSU Doctoral Dissertations

Schrenkiella parvula is an extremophyte model related to the most widely studied plant model, Arabidopsis thaliana and Brassica crops in the mustard family (Brassicaceae). It can thrive in highly saline environments where the soil is enriched in Na⁺, K⁺, Li⁺, borates, and chlorides. Understanding how this extremophyte can survive high salinity with genomic adaptations can provide insight into developing stress resilient crops in the future. Gene expression of S. parvula in response to salt has been investigated using shoot and root tissue from mature vegetative-phase plants. However, prior studies have not examined the transcript …

Environmental Controls On Carbon Biogeochemistry In Aquatic Systems, Lee Potter

LSU Master's Theses

Carbon dioxide (CO₂) outgassing from inland waters is a small but critical component of the global carbon cycle that is currently not well constrained. This thesis is composed of three interrelated studies with an overarching goal to further determine environmental factors impacting carbon dynamics in rivers and lakes. These studies utilized water samples and in situ measurements of pCO₂ and ambient water parameters from the Lower Mississippi River at Baton Rouge and University Lake in Baton Rouge Louisiana, USA collected between January 2021 and August 2022. Additional Meteorological and hydrological data were download from public sources. Results …

Dynamics Of Bottlebrush Polymers In Solution By Neutron Spin Echo Spectroscopy, Karin J. Bichler, Bruno Jakobi, Dirk Honecker, Laura R. Stingaciu, Thomas K. Weldeghiorghis, James H.P. Collins, Gerald J. Schneider

Faculty Publications

We present dynamical and morphological studies on poly(dimethylsiloxane) (PDMS)-based bottlebrush polymers in solution. A combination of small-angle neutron scattering (SANS), pulsed field gradient nuclear magnetic resonance (PFG NMR), and neutron spin echo (NSE) spectroscopy was used to identify structural changes inherent in changing the side-chain length while keeping the backbone constant. These morphological changes are mirrored in the diffusion coefficient determined by PFG NMR, resulting in two coefficients for the elongated and one for the more spherical sample. Faster motions are tracked by NSE using the advantage of time- and length-scale resolutions with the diffusion coefficient predetermined by PFG NMR. …

Defining Understudied Mechanisms Of Potassium Ion Flux In The Insect Nervous System, Rui Chen

LSU Doctoral Dissertations

To identify novel insecticide target sites that can address the evolution of insecticide resistance, our group has tested the insecticidal potential of two potassium flux pathways: potassium-chloride (K+/Cl−) cotransporter (KCC) and inward rectifier potassium (Kir) channels. GABAergic signaling is the cornerstone of fast synaptic inhibition for neural signaling in arthropods; thus, the γ-aminobutyric acid (GABA)-receptor-chloride-channel (GRCC) complex has been a longstanding target for insecticides. Despite the importance to neuronal homeostasis, the upstream mechanisms enabling GRCC function are poorly defined. The data presented in this dissertation provide strong support that KCC is an essential ion transport system for proper neural function …

The Applied Use Of Zein Nanoparticles In Agriculture, Colin Bonser

LSU Doctoral Dissertations

Nanotechnology is a multifaceted and a promising strategy in agricultural pest management for the development of insecticides or insecticide carriers. Research has shown that nanoparticles increase stability and efficacy of entrapped chemical pesticides to prevent active ingredient degradation while improving chemical stability and solubility. The following work evaluated nanoparticles synthesized from biopolymers, materials that originate from renewable sources also have an increased biodegradability.
Work was conducted under laboratory, greenhouse, and field conditions to test the biopoloymers as possible insecticide and insecticide carriers. The primary biopolymer evaluated in the following studies was zein, although lignin and chitosan were also evaluated. Overall, …

Paraquat-Resistant Italian Ryegrass (Lolium Multiflorum) Confirmed In Louisiana, Alphonse B. Coco Iii

LSU Master's Theses

Paraquat is the only herbicide labeled for postemergence control of Italian ryegrass in sugarcane. In 2017, sugarcane farmers in Louisiana reported Italian ryegrass control failures with paraquat applications. Seeds were collected from sugarcane fields in White Castle, St. Gabriel, and Bunkie, Louisiana where control was not achieved (reduced susceptibility). Additionally, Italian ryegrass seeds were collected from a sugarcane field in Welcome, LA with a history of paraquat use and with no reported control issues (susceptible population). A dose-response study was conducted at the Louisiana State University Agricultural Center’s Sugar Research Station in February 2021 and was repeated in November 2021. …

Esterase Mediated Insecticide Resistance In The Southern House Mosquito, Culex Quinquefasciatus, Vivek Pokhrel

LSU Doctoral Dissertations

Much is known about the development of insecticide resistance associated with targeted application against insect populations. However, off-target selection by applications of insecticides in agricultural and residential sites also impacts development of insecticide resistance and is understudied. Similarly, the impact of selecting one life stage of mosquitoes on the insecticide susceptibility of different life stages is largely unknown. The first part of this study shows that susceptibility to chlorantraniliprole, which is applied in rice and sugarcane fields in LA, decreased (5.7 to12-fold) in populations of Culex quinquefasciatus collected from near these fields compared to a reference field strain. In addition, …

Design, Synthesis, And Characterization Of Nqo1-Activatable Luminescent Probes, Ovini Vionika Weeratung Kankanamge

LSU Doctoral Dissertations

Being among the top ten causes of death, cancer has constantly been able to grab the attention of researchers all around the world. While several treatment methods prevail to cure cancer, the seriousness of this medical condition requires continuous work in the development of novel treatments as well as scientific advancements in order to achieve early detection of the disease. Luminescence imaging is an emerging field in both diagnosis of diseases, as well as a means to provide guidance in clinical surgeries. While there exist only a few contrasting agents approved by the Food and Drug Administration, the need for …

Role Of Cd38 In Pulmonary Host Defense Against Gram-Negative Pneumonia, Xiaoqian Shan

LSU Master's Theses

The Gram-negative bacterium, Klebsiella pneumoniae, is a major cause of hospital-acquired pneumonia in the U.S. In particular, the dramatic increase in carbapenem-resistant K. pneumoniae infections poses a serious threat to the public health both in the United States and worldwide. Clearance of bacteria in the lungs depends on effective pulmonary immune response. It may be possible to design improved therapies that augment host immune responses while attenuating excessive pulmonary inflammation through modulation of key innate immunity molecules during pneumonic infections. Cluster differentiation CD38 (CD38) has been detected on the surface of many immune cells or intracellular compartments, acting as …

Searching For The Soldier Pheromone: Behavioral Effects Of Soldiers And Soldier-Produced Chemicals In The Formosan Subterranean Termite, Coptotermes Formosanus (Blattodea: Rhinotermitidae)), Joseph Mccarthy

LSU Master's Theses

The Formosan subterranean termite, Coptotermes formosanus, is a eusocial insect and an invasive urban pest. Colonies function through a division of labor among castes (i.e., queens and kings, workers, and soldiers). Though soldiers are energetically expensive to maintain, C. formosanus has a relatively large soldier caste (~10% of the colony). Soldiers are physically unable to collect food or build tunnels but are regularly found with foraging workers. In some species, soldiers influence the worker foraging behavior. In C. formosanus, aside from defense, the roles soldiers play remains unknown. In this study, we determined if soldiers influence …

Effect Of Canopy Competition On Tree Growth And Development In Pine Hardwood Mixtures, Jacob Alexander Olichney

LSU Master's Theses

Mixed species forests may benefit from the reduction in intraspecific competition, although the benefit of species mixing has been shown to vary depending on mixture composition and environmental conditions. This study sought to test the effect of species composition in mixed forest stands on canopy development and growth of loblolly pine (Pinus taeda), cherrybark oak (Quercus pagoda), and sweetgum (Liquidambar styraciflua). Even-aged, grid planted monoculture and mixed species forest plots were established with hardwood monoculture plots at a 741 Trees Per Hectare (TPH), and pine monoculture and mixed species plots established at 1482 TPH. …

Enabling The Human Perception Of A Working Camera In Web Conferences Via Its Movement, Anish Shrestha

LSU Master's Theses

In recent years, video conferencing has seen a significant increase in its usage due to the COVID-19 pandemic. When casting user’s video to other participants, the videoconference applications (e.g. Zoom, FaceTime, Skype, etc.) mainly leverage 1) webcam’s LED-light indicator, 2) user’s video feedback in the software and 3) the software’s video on/off icons to remind the user whether the camera is being used. However, these methods all impose the responsibility on the user itself to check the camera status, and there have been numerous cases reported when users expose their privacy inadvertently due to not realizing that their camera is …

From Biowaste To Product: The Functionalization Of Biochar For Agricultural And Environmental Applications, Baoyue Zhou

LSU Doctoral Dissertations

Biochar is a porous carbonated substance that is manufactured through pyrolysis of biomass feedstocks in an oxygen-deficiency environment. The production of biochar from agricultural waste materials provides a potential solution for eliminating pollution while improving carbon sequestration. While biochar is shown to provide multiple benefits to soil health and fertility, its use in environmental remediation has been hampered by its low affinity for hazardous anionic species and the difficulty in isolating from water phase. To overcome these obstacles, various efforts have been made to modify biochar surface chemistry and morphology.

Fe/Mg co-doped biochar composites from sugarcane harvest residue were prepared …

Sers Platform For Single Fiber Endoscopic Probes, Debsmita Biswas

LSU Doctoral Dissertations

Molecular detection techniques have huge potential in clinical environments. In addition to many other molecular detection techniques, endoscopic Raman spectroscopy has great ability in terms of minimal invasiveness and real-time spectra acquisition. However, Raman Effect is low in sensitivity, limiting the application. Surface-Enhanced Raman Scattering (SERS), addresses this limitation. SERS brings rough nano-metallic surfaces in contact with specimen molecules which enormously enhances Raman signals. This provides Raman spectroscopy with immense capabilities for diverse fields of applications.

Generally, in clinical probe applications, the spectrometer is brought near the target molecules for detection. Typically, optical fibers are used to couple spectrometers to …

Assessing Turgor Loss Point In Bottomland Hardwood Trees Using Leaf Spectroscopy, Alexandra M. Eisley

LSU Master's Theses

Climate change is expected to radically alter our planet’s forests, with higher frequencies of drought- and flood-induced mortality events posing a challenge for forest managers and biologists. Research into the factors underlying plant tolerance to environmental stressors is therefore gaining popularity for incorporation into projective and earth system modeling using remote sensing measures. Leaf turgor loss point (TLP) is a key trait associated with drought tolerance among plants and is defined as the water potential at which leaf turgor pressure reaches zero, causing wilting. Here, I investigated patterns of TLP across the landscape and its role as an indicator of …

Spam Detection Using Machine Learning And Deep Learning, Olubodunde Agboola

LSU Doctoral Dissertations

Text messages are essential these days; however, spam texts have contributed negatively to the success of this communication mode. The compromised authenticity of such messages has given rise to several security breaches. Using spam messages, malicious links have been sent to either harm the system or obtain information detrimental to the user. Spam SMS messages as well as emails have been used as media for attacks such as masquerading and smishing ( a phishing attack through text messaging), and this has threatened both the user and service providers. Therefore, given the waves of attacks, the need to identify and remove …

The Role Of Kisspeptins In Trophoblast Cell Dynamics: Comparative Studies Using The Horse And The Preeclamptic-Like Bph/5 Mouse As Animal Models, Viviane Cristine Leite Gomes

LSU Doctoral Dissertations

Kisspeptins, a family of small peptides derived from the Kiss1 gene, have been described in trophoblast cell dynamics, placentation, and pregnancy outcomes. Furthermore, many other physiological functions have been attributed to kisspeptins, including neuroendocrine regulation of puberty and cyclicity, and peripheral and central regulation of metabolism and adipogenesis. Herein, studies were performed in the Blood Pressure High Subline 5 (BPH/5) mouse and the horse to further investigate the role of kisspeptins in pregnancy. The BPH/5 mouse recapitulates the main features of human preeclampsia (PE), a hypertensive disorder of pregnancy associated with maternal and fetal adverse outcomes. BPH/5 phenotypic characteristics include …

Interactive Industrial Energy Assessment Learning Platform Using 360-Degree Virtual Reality Contents, Laleh Ghanbari

LSU Doctoral Dissertations

The traditional passive industrial energy assessment training methods included low direct involvement of students, and they lacked an engaging learning environment. Since the main task for energy assessment is visual inspection and data collection, traditional passive training methods were not able to provide hands-on experience for the students. An active training method was needed to make the current industrial energy assessment training more efficient and effective, a method which provides the opportunity for students to have more interaction with the training environment. In this study, an interactive industrial energy assessment learning platform using 360ᵒ virtual reality contents was developed. The …