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Population Genomic Characterization Of Cercospora Janseana On Rice In The Southern United States, Jacob Searight

LSU Master's Theses

Cercospora janseana is the causative agent of narrow brown leaf spot (NBLS). Effort had been undertaken since the 1940’s to control this disease through resistance breeding in the Southern United States. However, a severe epidemic of NBLS in 2006 inspired renewed focus on managing this disease. One tool for managing this disease has been identified among resistant cultivars, the CRSP-2.1 resistance locus to NBLS. One thing limiting the investment and deployment of CRSP-2.1, and other unknown resistance genes, is the historical observation that resistance to NBLS rapidly breaks-down in the field. Together, the rapid breakdown of resistance to NBLS …

The Applied Use Of Zein Nanoparticles In Agriculture, Colin Bonser

LSU Doctoral Dissertations

Nanotechnology is a multifaceted and a promising strategy in agricultural pest management for the development of insecticides or insecticide carriers. Research has shown that nanoparticles increase stability and efficacy of entrapped chemical pesticides to prevent active ingredient degradation while improving chemical stability and solubility. The following work evaluated nanoparticles synthesized from biopolymers, materials that originate from renewable sources also have an increased biodegradability.
Work was conducted under laboratory, greenhouse, and field conditions to test the biopoloymers as possible insecticide and insecticide carriers. The primary biopolymer evaluated in the following studies was zein, although lignin and chitosan were also evaluated. Overall, …

Impact Of Rma’S Projected Price On Prevent Planting Claims, Nicolas Enrique Alvarez Mena

LSU Master's Theses

This research studies the presence of ex-post moral hazard in the prevent planting provision from the crop insurance program in cotton, corn, and soybeans for the Plains Region and Southeast Regions of the U.S. Three fixed effect models are developed using the proportion of prevent plant acres as the dependent variable, as well as weather, input price, expected harvest price and a break-even point for the independent variables. In addition, a fourth fixed effect (FE) model is introduced using the logit transformation and the same variables as in previous models. The results show the number of PP claims has been …

From Biowaste To Product: The Functionalization Of Biochar For Agricultural And Environmental Applications, Baoyue Zhou

LSU Doctoral Dissertations

Biochar is a porous carbonated substance that is manufactured through pyrolysis of biomass feedstocks in an oxygen-deficiency environment. The production of biochar from agricultural waste materials provides a potential solution for eliminating pollution while improving carbon sequestration. While biochar is shown to provide multiple benefits to soil health and fertility, its use in environmental remediation has been hampered by its low affinity for hazardous anionic species and the difficulty in isolating from water phase. To overcome these obstacles, various efforts have been made to modify biochar surface chemistry and morphology.

Fe/Mg co-doped biochar composites from sugarcane harvest residue were prepared …

The Role Of Kisspeptins In Trophoblast Cell Dynamics: Comparative Studies Using The Horse And The Preeclamptic-Like Bph/5 Mouse As Animal Models, Viviane Cristine Leite Gomes

LSU Doctoral Dissertations

Kisspeptins, a family of small peptides derived from the Kiss1 gene, have been described in trophoblast cell dynamics, placentation, and pregnancy outcomes. Furthermore, many other physiological functions have been attributed to kisspeptins, including neuroendocrine regulation of puberty and cyclicity, and peripheral and central regulation of metabolism and adipogenesis. Herein, studies were performed in the Blood Pressure High Subline 5 (BPH/5) mouse and the horse to further investigate the role of kisspeptins in pregnancy. The BPH/5 mouse recapitulates the main features of human preeclampsia (PE), a hypertensive disorder of pregnancy associated with maternal and fetal adverse outcomes. BPH/5 phenotypic characteristics include …

Identification Of Quantitative Trait Loci (Qtls) For Resistance To Bacterial Leaf Streak: Xanthomonas Translucens Using Qtl And Association Mapping In Three Populations Of Soft Red Winter Wheat, Benjamin Tyler Meritt

LSU Doctoral Dissertations

Bacterial leaf streak (BLS) and black chaff, caused by Xanthomonas translucens pv. undulosa (Xtu), can be a very destructive disease of wheat, especially in the warmer, wetter areas of the Southeastern U.S. Yield losses of up to 40 percent have been recorded in some cases in southern wheat growing regions. With no effective agronomic or chemical method of disease control, identification of genetic resistance is seen as a promising solution. Three soft red winter wheat populations (GAWN, ARK-SNP, and AGS 2060- AGS 2035 DH) representative of soft red winter wheat germplasm in the southeastern U.S. developed by …

Lethal Concentration And Sporulation By Contact And Direct Spray Of The Entomopathogenic Fungus Beauveria Bassiana On Different Stages Of Nezara Viridula (Heteroptera: Pentatomidae), Maribel Portilla, Minling Zhang, James Paul Glover, Gadi V.P. Reddy, Chris Johnson

Faculty Publications

The southern green stink bug, Nezara viridula (L.) (Heteroptera: Pentatomidae) is the most significant pest of soybean worldwide. The present study was conducted to compare the effectiveness of a Delta native strain NI8 of Beauveria bassiana by contact and direct spray on nymphs (2nd to 5th instar) and adults of N. viridula. Water control and four concentrations of B. bassiana were used to evaluate the survival, mortality, and molting percentage and to estimate median lethal concentration (LC50), median lethal sporulation (LS50), and resistance ratio (RR50). Direct spray at all concentrations observed the greatest reduction in survival on all life stages. …

Global Matrix 4.0 Physical Activity Report Card Grades For Children And Adolescents: Results And Analyses From 57 Countries, Salomé Aubert, Joel D. Barnes, Iryna Demchenko, Myranda Hawthorne, Chalchisa Abdeta, Patrick Abi Nader, José Carmelo Adsuar Sala, Nicolas Aguilar-Farias, Susana Aznar, Peter Bakalár, Jasmin Bhawra, Javier Brazo-Sayavera, Mikel Bringas, Jonathan Y. Cagas, Angela Carlin, Chen Kang Chang, Bozhi Chen, Lars Breum Christiansen, Candice Jo Anne Christie, Gabriela Fernanda De Roia, Christine Delisle Nyström, Yolanda Demetriou, Visnja Djordjic, Arunas Emeljanovas, Liri Findling Endy, Aleš Gába, Karla I. Galaviz, Silvia A. González, Kylie D. Hesketh, Wendy Yajun Huang, Omphile Hubona, Justin Y. Jeon, Danijel Jurakić, Jaak Jürimäe

Population and Public Health Science Faculty Publications

Background: The Global Matrix 4.0 on physical activity (PA) for children and adolescents was developed to achieve a comprehensive understanding of the global variation in children’s and adolescents’ (5–17 y) PA, related measures, and key sources of influence. The objectives of this article were (1) to summarize the findings from the Global Matrix 4.0 Report Cards, (2) to compare indicators across countries, and (3) to explore trends related to the Human Development Index and geo-cultural regions. Methods: A total of 57 Report Card teams followed a harmonized process to grade the 10 common PA indicators. An online survey was conducted …

Phytochemical Targeting Of Mitochondria For Breast Cancer Chemoprevention, Therapy, And Sensitization, Elizabeth R.M. Zunica, Christopher L. Axelrod, John P. Kirwan

Division of Basic Science Faculty Publications

Breast cancer is a common and deadly disease that causes tremendous physical, emotional, and financial burden on patients and society. Early-stage breast cancer and less aggressive subtypes have promising prognosis for patients, but in aggressive subtypes, and as cancers progress, treatment options and responses diminish, dramatically decreasing survival. Plants are nutritionally rich and biologically diverse organisms containing thousands of metabolites, some of which have chemopreventive, therapeutic, and sensitizing properties, providing a rich source for drug discovery. In this study we review the current landscape of breast cancer with a central focus on the potential role of phytochemicals for treatment, management, …

Multiscale Analysis Of Cancer Service Areas In The United States, Changzhen Wang, Tracy Onega, Fahui Wang

Faculty Publications

The purpose of delineating Cancer Service Areas (CSAs) is to define a reliable unit of analysis, more meaningful than geopolitical units such as states and counties, for examining geographic variations of the cancer care markets using geographic information systems (GIS). This study aims to provide a multiscale analysis of the U.S. cancer care markets based on the 2014–2015 Medicare claims of cancer-directed surgery, chemotherapy, and radiation. The CSAs are delineated by a scale-flexible network community detection algorithm automated in GIS so that the patient flows are maximized within CSAs and minimized between them. The multiscale CSAs include those comparable in …

Snapshots Of Coastal Ecology During Glacial And Deglacial Intervals: A Multiproxy Assessment Of Northern Gulf Of Mexico Sediment Cores, Kendall Emilie Brome

LSU Master's Theses

Terrestrial ecosystems along exposed continental shelves during times of relatively low sea level and glaciation are rarely preserved due to the mechanically erosive nature of marine transgression and regression. In this study, I investigate the geochemical and palynological characteristics of two well-preserved sites in the northern Gulf of Mexico (NGOM) to determine the ecologic settings and subsequent mechanisms of preservation in this region. Both sites contain preserved terrigenous sediments of previously exposed coastal margins; the first is located at the Alabama Underwater Forest (~13 km south of Gulf Shores, AL, at ~15 mbsl), and optically stimulated luminescence dates to 72–56 …

Polymer Mimetics For Soil Modeling And Detection Of Biomarkers, Md Ragib Hasan

LSU Doctoral Dissertations

The population of the world is increasing day by day and is expected to reach 9.8 billion by the year 2050. The ever-increasing demand for agricultural products is putting an unprecedented strain on the world's soils as the human population continues to expand. Soil degradation caused by over-farming and the agrochemicals (fertilizers, pesticides, etc.) used in agriculture is a growing problem, although its causes remain murky. In addition, little is understood about the molecular-level interactions of substances that are subsequently introduced to soils, such as agricultural chemicals (ACs). Therefore, it is expected that these constraints may be circumvented by the …

Understanding Motivations Among Sustained Cooperative Extension Partners For Obesity Prevention In Rural Louisiana, Catherine R. Losavio

LSU Master's Theses

Louisiana consistently has one of the highest rates of obesity in the nation with higher concentrations of obesity in many rural parishes (i.e., counties). Due to over a century of visibility and engagement in rural communities, Cooperative Extension (Extension) is uniquely poised to reach remote rural communities disproportionately impacted by obesity. As Extension increases its use of community coalitions and implements its new National Framework for Health Equity and Well-Being, understanding motivations and communication preferences among long-term rural coalition partners for obesity prevention and health promotion has become essential for duplicating successful local-level policy, system, and environmental (PSE) changes. This …

Influence Of Hesperidin On The Physico-Chemical, Microbiological, And Sensory Characteristics Of Frozen Yogurt, Roberto Antonio Cedillos Hernández

LSU Master's Theses

Frozen yogurts contain yogurt culture bacteria which might impart health benefits to its consumers. Global frozen yogurt market sales are expected to grow 4.8% by 2028 which represents an important opportunity for the industry, consumers, and researchers. Polyphenols are metabolites found in plants which have antioxidant and anti-inflammatory properties and might prevent chronical diseases such as cancer, diabetes, and cardiovascular diseases. The objective of this study was to elucidate the effect of the polyphenol hesperidin on the physico-chemical, microbiological, and sensory characteristics of frozen yogurts. Hesperidin was incorporated in frozen yogurt at three concentrations (500, 250 and 125 mg/90g of …

Evaluation Of Noise Regression Techniques In Resting-State Fmri Studies Using Data Of 434 Older Adults, Norman Scheel, Jeffrey N. Keller, Ellen F. Binder, Eric D. Vidoni, Jeffrey M. Burns, Binu P. Thomas, Ann M. Stowe, Linda S. Hynan, Diana R. Kerwin, Wanpen Vongpatanasin, Heidi Rossetti, C. Munro Cullum, Rong Zhang, David C. Zhu

Clinical Research Faculty Publications

Subject motion is a well-known confound in resting-state functional MRI (rs-fMRI) and the analysis of functional connectivity. Consequently, several clean-up strategies have been established to minimize the impact of subject motion. Physiological signals in response to cardiac activity and respiration are also known to alter the apparent rs-fMRI connectivity. Comprehensive comparisons of common noise regression techniques showed that the “Independent Component Analysis based strategy for Automatic Removal of Motion Artifacts” (ICA-AROMA) was a preferred pre-processing technique for teenagers and adults. However, motion and physiological noise characteristics may differ substantially for older adults. Here, we present a comprehensive comparison of noise-regression …

Novel Texture-Based Probabilistic Object Recognition And Tracking Techniques For Food Intake Analysis And Traffic Monitoring, Robert Jacob Dibiano

LSU Doctoral Dissertations

More complex image understanding algorithms are increasingly practical in a host of emerging applications. Object tracking has value in surveillance and data farming; and object recognition has applications in surveillance, data management, and industrial automation. In this work we introduce an object recognition application in automated nutritional intake analysis and a tracking application intended for surveillance in low quality videos. Automated food recognition is useful for personal health applications as well as nutritional studies used to improve public health or inform lawmakers. We introduce a complete, end-to-end system for automated food intake measurement. Images taken by a digital camera are …

Probiotic Characteristics Of Streptococcus Thermophilus And Lactobacillus Delbrueckii Ssp. Bulgaricus As Influenced By Carao (Cassia Grandis), David Paz, Ricardo S. Aleman, Roberto Cedillos, Douglas W. Olson, Kayanush Aryana, Jhunior Marcia, Charles Boeneke

Faculty Publications

Carao is considered a functional ingredient since its bioactive compounds are meaningful in nutritional, pharmacological, and medicinal applications. The objective of this study was to determine the effects of carao pulp powder on the bacterial viability, acid tolerance, bile tolerance, and protease activity of S. thermophilus STI-06 and L. bulgaricus LB-12. M17 broth with 0.5% lactose and MRS broth were used for S. thermophilus and L. bulgaricus, respectively, for determining bacterial viability, acid tolerance, and bile tolerance. Skim milk was used to study the protease activity of both bacteria. The carao was added at 0 (control), 1.3, 2.6, and 5.3 …

Single-Screen Bark Particle Separation Can Be Used To Engineer Stratified Substrate Systems, Jeb S. Fields, Kristopher S. Criscione, Ashley Edwards

Faculty Publications

Substrate stratification is an emerging substrate management strategy involving layering multiple substrate materials within a single container to modify physiochemical characteristics of the substrate system. Specifically, stratifying allows growers and researchers to rearrange the air–water balance within a container to modify hydraulic characteristics. Moreover, fertilizer can be incorporated into just the upper strata to reduce leaching. Research to date has shown benefits associated with resource efficiency, production timing, and weed control. With the associated benefits for substrate stratification, interested growers will need pragmatic solutions for onsite trials. Therefore, the objective of this study was to identify a cost-effective solution for …

Characterization Of Resistance To The Mexican Rice Borer (Lepidoptera: Crambidae) Among Sugarcane Cultivars, Leonardo D. Salgado, Blake E. Wilson, Hannah J. Penn, Randy T. Richard, Michael O. Way

Faculty Publications

Cultivar resistance is an essential management strategy for the Mexican rice borer, Eoreuma loftini (Dyar), in sugarcane in the USA, but resistance mechanisms are poorly understood. Resistance was evaluated among Louisiana’s (USA) commercial sugarcane cultivars and experimental clones through field screenings, greenhouse trials, and a diet incorporation assay. Cultivars L 01-299 and HoCP 85-845 had the lowest borer injury levels, while HoCP 00-950 and L 12-201 were among the most heavily injured in field and greenhouse trials. The variability of results between the two field trials suggests that a genotype × environment interaction might affect the expression of resistance. Oviposition …

Variability In Energy Expenditure Is Much Greater In Males Than Females, Lewis G. Halsey, Vincent Careau, Herman Pontzer, Philip N. Ainslie, Lene F. Andersen, Liam J. Anderson, Lenore Arab, Issad Baddou, Kweku Bedu-Addo, Ellen E. Blaak, Stephane Blanc, Alberto G. Bonomi, Carlijn V.C. Bouten, Pascal Bovet, Maciej S. Buchowski, Nancy F. Butte, Stefan G.J.A. Camps, Graeme L. Close, Jamie A. Cooper, Sai Krupa Das, Richard Cooper, Lara R. Dugas, Ulf Ekelund, Sonja Entringer, Terrence Forrester, Barry W. Fudge, Annelies H. Goris, Michael Gurven, Catherine Hambly, Asmaa El Hamdouchi, Marije B. Hoos, Sumei Hu, Noorjehan Joonas

Faculty Publications

In mammals, trait variation is often reported to be greater among males than females. However, to date, mainly only morphological traits have been studied. Energy expenditure represents the metabolic costs of multiple physical, physiological, and behavioral traits. Energy expenditure could exhibit particularly high greater male variation through a cumulative effect if those traits mostly exhibit greater male variation, or a lack of greater male variation if many of them do not. Sex differences in energy expenditure variation have been little explored. We analyzed a large database on energy expenditure in adult humans (1494 males and 3108 females) to investigate whether …

The Small Ras Superfamily Gtpase Rho4 Of The Maize Anthracnose Fungus Colletotrichum Graminicola Is Required For Β-1,3-Glucan Synthesis, Cell Wall Integrity, And Full Virulence, Ely Oliveira-Garcia, Lala Aliyeva-Schnorr, Alan De Oliveira Silva, Seif El Din Ghanem, Kathrin Thor, Edgar Peiter, Holger B. Deising

Faculty Publications

Small Ras superfamily GTPases are highly conserved regulatory factors of fungal cell wall biosynthesis and morphogenesis. Previous experiments have shown that the Rho4-like protein of the maize anthracnose fungus Colletotrichum graminicola, formerly erroneously annotated as a Rho1 protein, physically interacts with the β-1,3-glucan synthase Gls1 (Lange et al., 2014; Curr. Genet. 60:343–350). Here, we show that Rho4 is required for β-1,3-glucan synthesis. Accordingly, Δrho4 strains formed distorted vegetative hyphae with swellings, and exhibited strongly reduced rates of hyphal growth and defects in asexual sporulation. Moreover, on host cuticles, conidia of Δrho4 strains formed long hyphae with hyphopodia, rather than short …

Louisiana Agriculture Fall, 2022

Louisiana Agriculture

Livestock industries contribute $2.5 billion to the state’s economy, and beef cattle production is one of the most important sectors of the state’s agricultural enterprise. LSU AgCenter researchers study how to improve cattle production at research stations across the state. AgCenter Extension agents in every parish connect producers with their findings.

In this issue of Louisiana Agriculture magazine, livestock agents and research faculty describe the many programs they have developed to educate cattle producers and help 4-H’ers raise and show animals. Producers discuss what they have learned from Master Cattleman classes, and writer V. Todd Miller details how some cattle …

Social Influences On Physical Activity For Establishing Criteria Leading To Exercise Persistence, Ensela Mema, Everett S. Spain, Corby K. Martin, James O. Hill, R. Drew Sayer, Howard D. Mcinvale, Lee A. Evans, Nicholas H. Gist, Alexander D. Borowsky, Diana M. Thomas

Clinical Research Faculty Publications

Despite well-documented health benefits from exercise, a study on national trends in achieving the recommended minutes of physical activity guidelines has not improved since the guidelines were published in 2008. Peer interactions have been identified as a critical factor for increasing a population’s physical activity. The objective of this study is for establishing criteria for social influences on physical activity for establishing criteria that lead to exercise persistence. A system of differential equations was developed that projects exercise trends over time. The system includes both social and non-social influences that impact changes in physical activity habits and establishes quantitative conditions …

A Physical Activity Intervention In Older African Americans: The Paace Pilot Randomized Controlled Trial, Robert L. Newton, Robbie Beyl, Callie Hebert, Melissa Harris, Leah Carter, William Gahan, Owen Carmichael

Clinical Research Faculty Publications

Introduction Older African Americans have low levels of physical activity, which increases the risk of aging-related health conditions. This study aimed to determine the effectiveness of, and satisfaction with, a community-engaged physical activity intervention targeting older African Americans. Methods Fifty-six older African Americans were randomized to a successful aging group (SAG) or a physical activity group (PAG) for 12 wk. The PAG consisted of two weekly group exercise sessions and two to three home-based exercise sessions per week. The SAG consisted of weekly group educational sessions related to various aspects of healthy aging. Physical activity was measured by ActiGraph accelerometers …

Trends In Obesity Prevalence Among Children And Adolescents Aged 2 To 19 Years In The Us From 2011 To 2020, Kathy Hu, Amanda E. Staiano

Population and Public Health Science Faculty Publications

No abstract provided.

Contrasts Among Cationic Phytochemical Landscapes In The Southern United States, Luis Y. Santiago-Rosario, Kyle E. Harms, Dylan Craven

Faculty Publications

Understanding the phytochemical landscapes of essential and nonessential chemical elements to plants provides an opportunity to better link biogeochemical cycles to trophic ecology. We investigated the formation and regulation of the cationic phytochemical landscapes of four key elements for biota: Ca, Mg, K, and Na. We collected aboveground tissues of plants in Atriplex, Helianthus, and Opuntia and adjacent soils from 51, 131, and 83 sites, respectively, across the southern United States. We determined the spatial variability of these cations in plants and soils. Also, we quantified the homeostasis coefficient for each cation and genus combination, by using mixed-effect models, with …

Effects Of Soil And Foliar Applied Fertilizers On Growth, Yield And Sugar Quality Of Two Sugarcane Cultivars Under Rainfed Conditions, J. Bamrungrai, A. Polthanee, B. Tubana, V. Tre-Loges, A. Promkhambut

Faculty Publications

Background: In north-eastern Thailand, sugarcane is planted normally in late rainy season wherein the plants may experience drought stress during its early growth stage in dry season and waterlogging stress during late growth stage at peak of rainy season. Hence, the objective of the present study was to investigate the effects of soil application alone and soil combined with foliar application of nutrients on growth, yield and sugar quality of sugarcane grown under rainfed conditions. Methods: The field experiment was conducted during November 2016 to December 2017. A split-plot design with three replications was laid out. The two sugarcane cultivars …

Pest Population Dynamics Are Related To A Continental Overwintering Gradient, Douglas Lawton, Anders S. Huseth, George G. Kennedy, Amy C. Morey, William D. Hutchison, Dominic D. Reisig, Seth J. Dorman, De Shae Dillard, Robert C. Venette, Russell L. Groves, John J. Adamczyk, Izailda Barbosa Dos Santos, Tracey Baute, Sebe Brown, Eric Burkness, Ashley Dean, Galen P. Dively, Hélène B. Doughty, Shelby J. Fleischer, Jessica Green, Jeremy K. Greene, Krista Hamilton, Erin Hodgson, Thomas Hunt, David Kerns, Billy Rogers Leonard, Sean Malone, Fred Musser, David Owens, John C. Palumbo, Silvana Paula-Moraes, Julie A. Peterson, Ricardo Ramirez

Faculty Publications

Overwintering success is an important determinant of arthropod populations that must be considered as climate change continues to influence the spatiotemporal population dynamics of agricultural pests. Using a long-term monitoring database and biologically relevant overwintering zones, we modeled the annual and seasonal population dynamics of a common pest, Helicoverpa zea (Boddie), based on three overwintering suitability zones throughout North America using four decades of soil temperatures: the southern range (able to persist through winter), transitional zone (uncertain overwintering survivorship), and northern limits (unable to survive winter). Our model indicates H. zea population dynamics are hierarchically structured with continental-level effects that …

Effects Of Sensory Cues On Product Acceptability And Consumer Perceptions, Emotions, And Behavior, Cristhiam Gurdian Curran

LSU Doctoral Dissertations

Visual and cognitive cues can affect overall liking (OL) and consumer perceptions, emotions, and behavior. The first study explored the effect of product color difference on the liking, perception, and purchase intent (PI) of cheese-flavored-tortilla chips (CFTC) formulations (A and B) on serving plates (plastic, foam, and paper). Color differences between formulations influenced crunchiness and saltiness liking and perception, which together with overall flavor liking and formulation, mainly determined CFTC OL. Although having similar fracturability (N) and sodium content, formulation A had higher crunchiness and saltiness likings. PI was influenced by crunchiness, saltiness, and OF liking with 37, 49, and …

Endothelin-1 As A Novel Target For The Prevention Of Metabolic Dysfunction With Intermittent Hypoxia In Male Participants, Jacqueline K. Limberg, Sarah E. Baker, Humphrey G. Petersen-Jones, Winston Guo, An Huang, Michael D. Jensen, Prachi Singh

Clinical Research Faculty Publications

We examined the effect of intermittent hypoxia (IH, a hallmark feature of sleep apnea) on adipose tissue lipolysis and the role of endothelin-1 (ET-1) in this response. We hypothesized that IH can increase ET-1 secretion and plasma free fatty acid (FFA) concentrations. We further hypothesized that inhibition of ET-1 receptor activation with bosentan could prevent any IH-mediated increase in FFA. To test this hypothesis, 16 healthy male participants (32 ± 5 yr, 26 ± 2 kg/m2) were exposed to 30 min of IH in the absence (control) and presence of bosentan (62.5 mg oral twice daily for 3 days prior). …