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Bacillus Anthracis Virulence In Guinea Pigs Vaccinated With Anthrax Vaccine Adsorbed Is Linked To Plasmid Quantities And Clonality, Pamala R. Coker, Kimothy L. Smith, Patricia F. Fellows, Galena Rybachuck, Konstantin G. Kousoulas, Martin E. Hugh-Jones Mar 2003

Bacillus Anthracis Virulence In Guinea Pigs Vaccinated With Anthrax Vaccine Adsorbed Is Linked To Plasmid Quantities And Clonality, Pamala R. Coker, Kimothy L. Smith, Patricia F. Fellows, Galena Rybachuck, Konstantin G. Kousoulas, Martin E. Hugh-Jones

Faculty Publications

Bacillus anthracis is a bacterial pathogen of great importance, both historically and in the present. This study presents data collected from several investigations and indicates that B. anthracis virulence is associated with the clonality and virulence of plasmids pXO1 and pXO2. Guinea pigs vaccinated with Anthrax Vaccine Adsorbed were challenged with 20 B. anthracis isolates representative of worldwide genetic diversity. These same isolates were characterized with respect to plasmid copy number by using a novel method of quantitative PCR developed for rapid and efficient detection of B. anthracis from environmental samples. We found that the copy numbers for both pXO1 …

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Plasma Membrane Topology Of Syncytial Domains Of Herpes Simplex Virus Type 1 Glycoprotein K (Gk): The Ul20 Protein Enables Cell Surface Localization Of Gk But Not Gk-Mediated Cell-To-Cell Fusion, Timothy P. Foster, Xavier Alvarez, Konstantin G. Kousoulas Jan 2003

Plasma Membrane Topology Of Syncytial Domains Of Herpes Simplex Virus Type 1 Glycoprotein K (Gk): The Ul20 Protein Enables Cell Surface Localization Of Gk But Not Gk-Mediated Cell-To-Cell Fusion, Timothy P. Foster, Xavier Alvarez, Konstantin G. Kousoulas

Faculty Publications

Most spontaneously occurring mutations that cause extensive herpes simplex virus type 1 (HSV-1)-induced cell fusion are single amino acid changes within glycoprotein K (gK). Despite the strong genetic association of gK with virus-induced cell fusion, its direct involvement in cellular membrane fusion has been controversial, largely due to previously unsuccessful efforts to detect gK expression on virion and cellular surfaces. Recently, we showed that gK is expressed on HSV-1 virions and functioned in virus entry (T. P. Foster, G. V. Rybachuk, and K. G. Kousoulas, J. Virol. 75:12431-12438, 2001). To determine whether gK is expressed on cellular surfaces, as well …

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Natural Bovine Lentiviral Type 1 Infection In Holstein Dairy Cattle. I. Clinical, Serological, And Pathological Observations, T. G. Snider, B. F. Jenny, P. G. Hoyt, K. S. Coats, K. F. Graves, C. R. Cooper, R. W. Storts, D. G. Luther Jan 2003

Natural Bovine Lentiviral Type 1 Infection In Holstein Dairy Cattle. I. Clinical, Serological, And Pathological Observations, T. G. Snider, B. F. Jenny, P. G. Hoyt, K. S. Coats, K. F. Graves, C. R. Cooper, R. W. Storts, D. G. Luther

Faculty Publications

Clinical, serological, and pathological abnormalities observed in Holstein cows naturally infected with bovine lentivirus 1 bovine immunodeficiency virus (BIV) and other infections were progressive and most commonly associated with weight loss, lymphoid system deficiency, and behavioral changes. Clinical evidence of meningoencephalitis was dullness, stupor, and occasional head or nose pressing postures. The polymerase chain reactions associated the BIV provirus with the lesions in the central nervous system and lymphoid tissues. Multiple concurrent infections developed in retrovirally infected cows undergoing normal stresses associated with parturition and lactation. A major functional correlate of the lymphoreticular alterations was the development of multiple secondary …

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Heat Transfer In Outdoor Aquaculture Ponds, Jonathan Lamoureux Jan 2003

Heat Transfer In Outdoor Aquaculture Ponds, Jonathan Lamoureux

LSU Master's Theses

An energy balance was developed for heated and unheated earthen aquaculture ponds to 1) determine the relative importance of energy transfer mechanism affecting pond temperature; 2) predict pond temperatures; 3) estimate the energy required to control pond temperatures, and 4) recommend efficient heating and cooling methods. PHATR (Pond Heating and Temperature Regulation), a computer program using 4th order Runge-Kutta numerical method was developed to solve the energy balance using weather, flow rate and pond temperature data. By comparing measured and modeled pond temperatures, the average difference (the average bias) was 0.5°C for unheated ponds and 2.4°C for heated ponds. The …

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The Body Politic: Burial And Post-War Reconciliation In Baton Rouge, Leah Wood Jewett Jan 2003

The Body Politic: Burial And Post-War Reconciliation In Baton Rouge, Leah Wood Jewett

LSU Master's Theses

Historians typically agree that reconciliation between the white North and South took place between the period of 1898 (Spanish-American War) and 1913 (before World War I). To test this hypothesis and identify when reconciliation took place in Baton Rouge, Louisiana, I will use the burial of R. L. Pruyn in the Baton Rouge National Cemetery. Pruyn served as a U.S. soldier during the Mexican War and a Confederate soldier during the Civil War. Anthropologists have studied rituals, beliefs, and practices associated with death since early in the discipline. Archaeologists, in particular, have focused on this aspect of culture, in large …

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Use Of Urine Ph To Predict Incidence Of Ketosis In Transition Dairy Cows, Amy Elizabeth Beem Jan 2003

Use Of Urine Ph To Predict Incidence Of Ketosis In Transition Dairy Cows, Amy Elizabeth Beem

LSU Master's Theses

Forty-one (10 primiparous and 31 multiparous) Holstein cows were used in an experiment to determine the effects of prepartum dietary energy (PDE) and supplemental Ca-propionate on the incidence of ketosis in transition dairy cows and to evaluate the usefulness of urine pH as a predictor for ketosis. Treatment factors were prepartum dietary energy concentration (normal vs. high) with or without 113.5g Ca-Propionate/day (Nutro Cal®, Kemin Industries, Inc., Des Moines, IA). All cows were fed the same basal diet postpartum. Ca-propionate supplementation continued postpartum. Cows were individually fed and dry matter intakes (DMI) were measured daily. Milk production was measured and …

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The Effect Of Aging On The Immune Response To Vaccination In The Horse, Daniel H. Fermaglich Jan 2003

The Effect Of Aging On The Immune Response To Vaccination In The Horse, Daniel H. Fermaglich

LSU Master's Theses

Vaccination programs are designed to protect an animal from infection, however, depending upon the age and health of the animal vaccination may not stimulate a protective humoral response. It is possible that, as in the human and mouse models, geriatric equines may be less responsive than their younger counterparts to current vaccination protocols. The purpose of this study was to identify an age related diminution in the primary and secondary immune responses of geriatric horses in response to vaccination. Two groups of horses were sampled. The first group consisted of an open herd of 39 privately owned horses, varying in …

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An Anthropological Examination Of Classic Maya Burials From Moho Cay, Belize: Skeletal And Dental Evidence Of Demography, Diet And Health, Erin Suzanne Lund Jan 2003

An Anthropological Examination Of Classic Maya Burials From Moho Cay, Belize: Skeletal And Dental Evidence Of Demography, Diet And Health, Erin Suzanne Lund

LSU Master's Theses

In the Maya area, archaeological excavations have yielded an abundance of skeletal material from a multitude of sites throughout Belize, Guatemala, Mexico, El Salvador and Honduras. In 1979, a series of excavations led by Heather McKillop at Moho Cay, Belize, revealed a number of human interments. This thesis analyzes the Moho Cay skeletal collection composed of remains from eight discrete Late Classic (A.D. 600-800) burials and nine other archaeological units. Skeletal and dental analysis of the remains included assessing minimum number of individuals per burial and establishing age and sex for each individual. The bones were also examined for signs …

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Equine Immunity To Cyathostome Infections, Marie Alexandra Baudena Jan 2003

Equine Immunity To Cyathostome Infections, Marie Alexandra Baudena

LSU Doctoral Dissertations

To study the protective responses of cyathostome-infected ponies, two challenges were performed employing animals with different histories of exposure to these parasites. The hypothesis developed and to be tested in these experiments was that ponies that had longer exposure to cyathostome contaminated pastures would express acquired resistance to infection. The assumption behind this hypothesis was that helminth-naïve ponies infected with cyathostomes would eliminate the infection using only innate immune responses. Whereas previously exposed ponies would eliminate the infection with acquired immune responses, and these would be more effective in ponies with longer exposure to cyathostomes. Thus, helminth-naïve animals would acquire …

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Immunological, Hematological, And Serum Biochemical Effects Of High Level Dietary Fish Oil And Vitamin E Supplementation In The Dog, Casey J. Leblanc Jan 2003

Immunological, Hematological, And Serum Biochemical Effects Of High Level Dietary Fish Oil And Vitamin E Supplementation In The Dog, Casey J. Leblanc

LSU Doctoral Dissertations

Inflammation is a component of the innate immune response. However, severe or prolonged inflammation can be detrimental. Dietary n-3 fatty acid supplementation has proven clinical benefits in chronic inflammatory diseases, most likely due to reduced synthesis of inflammatory mediators and inhibition of lymphocyte proliferation and function. The purposes of this study were to characterize alterations in inflammatory mediator production, and lymphocyte proliferation, in dogs fed a diet consisting of 0.65% n-3 fatty acids (DMB) with an n-6:n-3 fatty acid ratio of 3.4:1. Fifteen dogs were randomly assigned to one of three dietary groups for twelve weeks. Group Sunflower oil received …

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The Epidemiology Of Shiga-Toxigenic Escherichia Coli O157:H7 In Louisiana Cattle, And White-Tailed Deer, John Robert Dunn Jan 2003

The Epidemiology Of Shiga-Toxigenic Escherichia Coli O157:H7 In Louisiana Cattle, And White-Tailed Deer, John Robert Dunn

LSU Doctoral Dissertations

Shiga-toxigenic Escherichia coli (STEC) O157:H7 is an important human pathogen. Dairy cattle, beef cattle, and white-tailed deer (WTD) are involved in STEC O157:H7 transmission to humans. We conducted cross-sectional studies in Louisiana, using sensitive microbiological methods, in dairy cattle, beef cattle, and WTD to investigate the epidemiology of STEC O157:H7. Results of the dairy studies are as follows. In the point prevalence study, summer-time prevalence in herds was 38.5%, with a cow-level prevalence of 6.5%. Among positive herds, cow-level prevalence ranged from 3%-34.6%. Three of five herds sampled in the longitudinal study were positive. Cow-level prevalence increased during spring and …

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Optimization And Automation Of A Thermal Oyster Shucking Process, Daniel Edgar Martin Jan 2003

Optimization And Automation Of A Thermal Oyster Shucking Process, Daniel Edgar Martin

LSU Doctoral Dissertations

Louisiana Gulf Coast oysters (Crassostrea virginica) were subjected to thermal shucking treatments to effect adductor muscle release from both left and right valves. The oysters were instrumented with thermocouples to monitor and record process temperatures in the oysters and on the shell. Following treatment, the oysters were evaluated for relaxation and release of the adductor muscle, meat quality and texture, and for the effect of the treatments on the storage life of the oysters as measured by total microbial plate counts. The treatments of many oysters resulted in a complete release of the adductor muscle from the shell while maintaining …

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Susceptibility Of Bacillus Anthracis To Gamma And Cherry Bacteriophage, Preston A. Fulmer Jan 2003

Susceptibility Of Bacillus Anthracis To Gamma And Cherry Bacteriophage, Preston A. Fulmer

LSU Master's Theses

Bacillus anthracis is a bacterium that causes severe disease mainly in ruminants, but can affect any mammal, including humans. A popular method for the detection of this organism is susceptibility of the bacterial isolate to g bacteriophage. However, to date no study on the resistance of a wide variety of B. anthracis isolates has been conducted. The following study examines the rate of resistance of a wide range of B. anthracis isolates to g phage as well as another phage specific for B. anthracis known as Cherry phage. We also compared susceptibility to phage with another detection method, susceptibility to …

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Psychosocial Predictors Of Visceral Adiposity, Paula C. Rhode Jan 2003

Psychosocial Predictors Of Visceral Adiposity, Paula C. Rhode

LSU Doctoral Dissertations

Psychosocial factors are thought to influence health through primarily direct physiological mechanisms or the alteration of health related behaviors. Three factors hypothesized to negatively impact health include arousal, life stress, and depressive symptomatology. One recent theorist suggests that the interaction between psychological stress and stress hormones on the neuroendocrine system may result in adverse changes to body composition, most notably the increased deposition of visceral adipose tissue (Bjorntorp, 1993). The current study prospectively examined the relationship between self-reported stressful life events, depressive symptoms and trait arousal on the deposition of visceral fat, as measured by computerized tomography (CT). Subjects were …

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Developing Risk Assessment Maps For Schistosoma Haematobium In Kenya Based On Climate Grids And Remotely Sensed Data, Kelsey Lee Mcnally Jan 2003

Developing Risk Assessment Maps For Schistosoma Haematobium In Kenya Based On Climate Grids And Remotely Sensed Data, Kelsey Lee Mcnally

LSU Master's Theses

It is important to be able to predict the potential spread of water borne diseases when building dams or redirecting rivers. This study was designed to test whether the use of a growing degree day (GDD) climate model and remotely sensed data (RS) within a geographic information system (GIS), could be used to predict both the distribution and severity of Schistosoma haematobium. Growing degree days are defined as the number of degrees centigrade over the minimum temperature required for development. The base temperature and the number of GDD required to complete one generation varies for each species. A monthly climate …

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Identification Of A Streptolysin S-Associated Gene Cluster And Its Role In The Pathogenesis Of Streptococcus Iniae Disease, Jeffrey D. Fuller, Alvin C. Camus, Carla L. Duncan, Victor Nizet, Darrin J. Bast, Ronald L. Thune, Donald E. Low, Joyce C.S. De Azavedo Oct 2002

Identification Of A Streptolysin S-Associated Gene Cluster And Its Role In The Pathogenesis Of Streptococcus Iniae Disease, Jeffrey D. Fuller, Alvin C. Camus, Carla L. Duncan, Victor Nizet, Darrin J. Bast, Ronald L. Thune, Donald E. Low, Joyce C.S. De Azavedo

Faculty Publications

Streptococcus iniae causes meningoencephalitis and death in cultured fish species and soft-tissue infection in humans. We recently reported that S. iniae is responsible for local tissue necrosis and bacteremia in a murine subcutaneous infection model. The ability to cause bacteremia in this model is associated with a genetic profile unique to strains responsible for disease in fish and humans (J. D. Fuller, D. J. Bast, V. Nizet, D. E. Low, and J. C. S. de Azavedo, Infect. Immun. 69:1994-2000, 2001). S. iniae produces a cytolysin that confers a hemolytic phenotype on blood agar media. In this study, we characterized the …

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Comparative Proteome Analysis Of Brucella Melitensis Vaccine Strain Rev 1 And A Virulent Strain, 16m, Michel Eschenbrenner, Mary Ann Wagner, Troy A. Horn, Jo Ann Kraycer, Cesar V. Mujer, Sue Hagius, Philip Elzer, Vito G. Delvecchio Sep 2002

Comparative Proteome Analysis Of Brucella Melitensis Vaccine Strain Rev 1 And A Virulent Strain, 16m, Michel Eschenbrenner, Mary Ann Wagner, Troy A. Horn, Jo Ann Kraycer, Cesar V. Mujer, Sue Hagius, Philip Elzer, Vito G. Delvecchio

Faculty Publications

The genus Brucella consists of bacterial pathogens that cause brucellosis, a major zoonotic disease characterized by undulant fever and neurological disorders in humans. Among the different Brucella species, Brucella melitensis is considered the most virulent. Despite successful use in animals, the vaccine strains remain infectious for humans. To understand the mechanism of virulence in B. melitensis, the proteome of vaccine strain Rev 1 was analyzed by two-dimensional gel electrophoresis and compared to that of virulent strain 16M. The two strains were grown under identical laboratory conditions. Computer-assisted analysis of the two B. melitensis proteomes revealed proteins expressed in either 16M …

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Xeriscape Guidelines Adapted To Residential Gardens In Cyprus, Elli George Georgiou Jan 2002

Xeriscape Guidelines Adapted To Residential Gardens In Cyprus, Elli George Georgiou

LSU Master's Theses

One of the major problems that my country, Cyprus, faces is water scarcity. In the last five to ten years, the problem has become more serious because of a series of droughts that have left the island with limited water. As a result, Cypriots keep their gardens to a minimum or they abandon their gardens due to water shortage. This attitude of Cypriots toward their gardens was the inspiration of this thesis topic. The idea of Xeriscape and the seven guidelines of Xeriscape that were first introduced and organized by the Denver Water Department in Colorado are directly related to …

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The Changing Face Of Hiv/Aids: An Anthropological And Epidemiological Study Of The Baton Rouge Area, Erica Brooke Gibson Jan 2002

The Changing Face Of Hiv/Aids: An Anthropological And Epidemiological Study Of The Baton Rouge Area, Erica Brooke Gibson

LSU Master's Theses

The Human Immunodeficiency Virus (HIV) and the resulting Acquired Immune Deficiency Syndrome (AIDS) became widespread in the early 1980’s. At the beginning of the epidemic, HIV/AIDS was affecting mainly gay men. As the disease began to spread, more diverse populations were affected. Now, two decades later, the face of HIV and AIDS has changed. In the year 2000, the Baton Rouge area (which includes the parishes of Ascension, East Baton Rouge, East Feliciana, Iberville, Pointe Coupee, West Baton Rouge, and West Feliciana) had the highest detected rate of HIV/AIDS cases in the state, and the 16th highest detected rate of …

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Fecal Coliform Concentration In Surface Runoff From Pastures With Applied Dairy Manure, Alyson Kristine Bertges Hubbs Jan 2002

Fecal Coliform Concentration In Surface Runoff From Pastures With Applied Dairy Manure, Alyson Kristine Bertges Hubbs

LSU Master's Theses

This study examined the magnitude of release of fecal coliform indicator bacteria in surface runoff from pasture amended with dairy manure. The experiment was conducted at Southeast Research Station in Franklinton, Louisiana between March and June, 2001. The experiment was designed to compare the effects of two methods of manure application on fecal coliform release. Manure application simulating natural deposition by cattle (Treatment A) was compared to that of land application of manure as fertilizer (Treatment B). Application of inorganic fertilizer was used as the control. Each Treatment was applied to three field plots. Simulated rainfalls were conducted on plots …

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Nursing Students' Knowledge, Observation Of Environmental Risk Factors And Compliance With Recommended Precautions For The Prevention Of Transmission Of Infectious Diseases By Needlestick Injury, Cynthia Ann Logan Jan 2002

Nursing Students' Knowledge, Observation Of Environmental Risk Factors And Compliance With Recommended Precautions For The Prevention Of Transmission Of Infectious Diseases By Needlestick Injury, Cynthia Ann Logan

LSU Doctoral Dissertations

The primary purpose of this study was to determine the relationships between selected institutional and personal demographic factors, knowledge, observation of environmental risk factors, and the degree of nursing student compliance with standard and transmission-based precautions for the prevention of transmission of infectious diseases. A four part, 89 item questionnaire was administered to students enrolled in clinical courses at three associate degree and four baccalaureate nursing programs in order to measure knowledge, compliance, environmental risk factors, and demographics. Useable data was collected from 710 nursing students enrolled in clinical courses. The typical respondent was female, between the ages of 20 …

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Nursing As Social Responsibility: Implications For Democracy From The Life Perspective Of Lavinia Lloyd Dock (1858-1956), Soledad Mujica Smith Jan 2002

Nursing As Social Responsibility: Implications For Democracy From The Life Perspective Of Lavinia Lloyd Dock (1858-1956), Soledad Mujica Smith

LSU Doctoral Dissertations

This dissertation focuses on Lavinia Lloyd Dock's (1858-1956) re-envisioning of nursing and caring as social responsibility and the implications of this conceptualization for democracy. Dock was an American nurse, educator, settlement worker, suffragist, pacifist, social activist, writer, and historian. Her conception of holistic welfare embodied a 'new ideal' of society (Dock 1907, p. 899), a new understanding of democracy, and an expression of citizenship based on social responsibility for the welfare of others. Dock's idea of democracy embraced women's values and ways of being in the world; disputed universal, individual rights; and privileged communal values, collaboration, inclusion, and diversity. Moreover, …

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Production Of Transgenic Goats By Somatic Cell Nuclear Transfer, Brett C. Reggio Jan 2002

Production Of Transgenic Goats By Somatic Cell Nuclear Transfer, Brett C. Reggio

LSU Doctoral Dissertations

A series of experiments were conducted to produce transgenic goats by somatic cell nuclear transfer (NT). In all experiments, donor cells were electrically fused to enucleated metaphase II oocytes, then chemically activated. In a preliminary study to evaluate embryonic development following NT with proliferating or quiescent fibroblast or cumulus cells, significantly more embryos reconstructed with quiescent cumulus cells fused (77%) compared with proliferating cumulus cells (41%), proliferating fibroblasts (36%) or quiescent fibroblasts (37%). Improved development to the eight- to sixteen-cell stage was observed when fibroblast cells were serum starved (serum starved 39% vs. serum fed 15%). However, there was no …

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Analysis Of Death Trends In Coroner Cases In East Baton Rouge Parish From January 1, 1991 To December 31, 2001, Gina Ann Dimattia Jan 2002

Analysis Of Death Trends In Coroner Cases In East Baton Rouge Parish From January 1, 1991 To December 31, 2001, Gina Ann Dimattia

LSU Master's Theses

Analysis of death trends in a geographic area can provide valuable information regarding the health of the people in that area. Additionally, evaluation of variables such as sex, race, age, and cause of death can aid local health officials in serving the needs of the population. East Baton Rouge Parish is located in southern Louisiana, includes the capital city of Baton Rouge, and has a population of 412,852. An analysis of death cases handled by the Coroner's Office in East Baton Rouge Parish has not been performed until now. This thesis presents results from an analysis of all deaths in …

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It Came From Outer Space: The Virus, Cultural Anxiety, And Speculative Fiction, Anne-Marie Thomas Jan 2002

It Came From Outer Space: The Virus, Cultural Anxiety, And Speculative Fiction, Anne-Marie Thomas

LSU Doctoral Dissertations

This study seeks to explore and interrogate the “viral reality” of the 1990s, in which the virus, heavily indebted to representations of AIDS for its metaphorical power, emerged as a prominent agent in science and popular culture. What becomes apparent in both fictional and non-fictional texts of this era, however, is that the designation of “virus” transcends specific and material viral phenomena, making the virus itself a touchstone for modern preoccupations with self and other. As constituted by the human body’s interaction with pathogenic agents, the binary of self and other may be deconstructed by an interrogation of the virus …

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Somatic Complaints And Health Care Utilization In Children Exposed To Violence, Kellie Ann Hilker Jan 2002

Somatic Complaints And Health Care Utilization In Children Exposed To Violence, Kellie Ann Hilker

LSU Doctoral Dissertations

In the United States, violence has been referred to as a public health epidemic and violence exposure of our youth is a particularly serious national concern. Numerous negative outcomes are associated with both child victimization and violence exposure including externalizing problems, internalizing symptoms, and poor academic performance. Somatic complaints, which have been associated with internalizing symptoms, have been found as well; however, physical complaints have not been studied in depth. Additionally, health care utilization is an area that is not widely studied in pediatric psychology, but positive associations between posttraumatic stress disorder and increased health care utilization has been found …

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Antipredator Conditioning In Mississippi Sandhill Cranes (Grus Canadensis Pulla), Jennifer Jill Heatley Jan 2002

Antipredator Conditioning In Mississippi Sandhill Cranes (Grus Canadensis Pulla), Jennifer Jill Heatley

LSU Master's Theses

The Mississippi Sandhill Crane (Grus canadensis pulla), the most endangered North American crane, is considered critically endangered and is protected by Federal and State law. Substantial funding has established the Mississippi Sandhill Crane National Wildlife Refuge in Southern Mississippi and an artificial insemination / breeding facility at the Audubon Center for Research of Endangered Species in New Orleans, Louisiana to promote species recovery. In spite of extensive time, labor, and money invested in captive propagation, juvenile Mississippi Sandhill Cranes suffer substantial mortality due to predation by bobcats (Lynx rufus), coyotes (Canis latrans) and red tailed hawks (Buteo jamaicensis) upon reintroduction …

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Glycoprotein K Specified By Herpes Simplex Virus Type 1 Is Expressed On Virions As A Golgi Complex-Dependent Glycosylated Species And Functions In Virion Entry, T. P. Foster, G. V. Rybachuk, K. G. Kousoulas Dec 2001

Glycoprotein K Specified By Herpes Simplex Virus Type 1 Is Expressed On Virions As A Golgi Complex-Dependent Glycosylated Species And Functions In Virion Entry, T. P. Foster, G. V. Rybachuk, K. G. Kousoulas

Faculty Publications

To facilitate detection of glycoprotein K (gK) specified by herpes simplex virus, a 12-amino-acid epitope tag was inserted within gK domain III. Recombinant virus gKprotC-DIII, expressing the tagged gK, was isolated. This virus formed wild-type plaques and replicated as efficiently as the wild-type KOS virus in Vero cells. Anti-protein C MAb detected high-mannose and Golgi complex-dependent glycosylated gK within cells as well as on purified virions. The gK-null virus ΔgK (gK-/-) entered Vero cells substantially more slowly than the wild-type KOS (gK+/+), while ΔgK virus grown in complementing VK302 cells (gK-/+) entered with entry kinetics similar to those of the …

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Fusobacteriosis In Captive Wild-Caught Pronghorns (Antilocapra Americana), J. F. Edwards, D. S. Davis, T. J. Roffe, F. Ramiro-Ibañez, P. H. Elzer Dec 2001

Fusobacteriosis In Captive Wild-Caught Pronghorns (Antilocapra Americana), J. F. Edwards, D. S. Davis, T. J. Roffe, F. Ramiro-Ibañez, P. H. Elzer

Faculty Publications

An outbreak of Fusobacterium necrophorum-induced septicemia occurred in a group of 40 captive wild-caught pronghorns (Antilocapra americana). Primary pododermatitis or necrotic stomatitis progressed to produce fatal septicemia with metastatic lesions in the forestomachs, lung, liver, and cecum in 38 of the animals. Two remaining animals were euthanatized because of chronic pododermatitis. Housing the animals in a pasture previously used by bovids and heavy rains with persistence of ground water pools in the pasture were contributing factors in the pathogenesis of this outbreak.

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An Α-Helical Domain Within The Carboxyl Terminus Of Herpes Simplex Virus Type 1 (Hsv-1) Glycoprotein B (Gb) Is Associated With Cell Fusion And Resistance To Heparin Inhibition Of Cell Fusion, Timothy P. Foster, Jeff M. Melancon, Konstantin G. Kousoulas Aug 2001

An Α-Helical Domain Within The Carboxyl Terminus Of Herpes Simplex Virus Type 1 (Hsv-1) Glycoprotein B (Gb) Is Associated With Cell Fusion And Resistance To Heparin Inhibition Of Cell Fusion, Timothy P. Foster, Jeff M. Melancon, Konstantin G. Kousoulas

Faculty Publications

Previous studies from our laboratory indicated that a 28-amino-acid carboxyl-terminal truncation of gB caused extensive virus-induced cell fusion (Baghian et al., 1993, J Virol 67, 2396-2401). We tested the ability of additional truncations and mutations within gB to cause cell fusion in the recently established virus-free cell fusion assay (Turner et al., 1998, J. Virol. 72, 873-875). Deletion of the carboxyl-terminal 28 amino acids of gB (gBΔ28), which removed part of the predicted α-helical structure H17b, caused extensive cell fusion. A gB truncation specified by gBΔ36, which removed the entire H17b domain, caused as much cell fusion as the gBΔ28 …

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