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Evaluating The Spatial Ecology Of Anthrax In North America: Examining Epidemiological Components Across Multiple Geographic Scales Using A Gis-Based Approach, Jason Kenna Blackburn Jan 2006

Evaluating The Spatial Ecology Of Anthrax In North America: Examining Epidemiological Components Across Multiple Geographic Scales Using A Gis-Based Approach, Jason Kenna Blackburn

LSU Doctoral Dissertations

This dissertation explores the spatial ecology and potential pathways of infection of anthrax, Bacillus anthracis, in North America. A multi-scale approach was used to evaluate the components required for disease agent survival in the environment, interactions with wildlife, and the potential role that vectors play in anthrax transmission. First, ecological niche modeling with the Genetic Algorithm for Rule-set Production (GARP) was used to predict the geographic distribution of anthrax in the continental U.S. using case data from outbreaks between 1957 and 2005. These results were then used to produce the first quantitative, continental scale predictions of anthrax in Mexico. At …

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Host-Parasitoid Spatial Ecology: A Plea For A Landscape-Level Synthesis, James T. Cronin, John D. Reeve Nov 2005

Host-Parasitoid Spatial Ecology: A Plea For A Landscape-Level Synthesis, James T. Cronin, John D. Reeve

Faculty Publications

A growing body of literature points to a large-scale research approach as essential for understanding population and community ecology. Many of our advances regarding the spatial ecology of predators and prey can be attributed to research with insect parasitoids and their hosts. In this review, we focus on the progress that has been made in the study of the movement and population dynamics of hosts and their parasitoids in heterogeneous landscapes, and how this research approach may be beneficial to pest management programs. To date, few studies have quantified prey and predator rates and ranges of dispersal and population dynamics …

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The Case For Selection At Ccr5-Δ32, Pardis C. Sabeti, Emily Walsh, Steve F. Schaffner, Patrick Varilly, Ben Fry, Holli B. Hutcheson, Mike Cullen, Tarjei S. Mikkelsen, Jessica Roy, Nick Patterson, Richard Cooper, David Reich, David Altshuler, Stephen O'Brien, Eric S. Lander Nov 2005

The Case For Selection At Ccr5-Δ32, Pardis C. Sabeti, Emily Walsh, Steve F. Schaffner, Patrick Varilly, Ben Fry, Holli B. Hutcheson, Mike Cullen, Tarjei S. Mikkelsen, Jessica Roy, Nick Patterson, Richard Cooper, David Reich, David Altshuler, Stephen O'Brien, Eric S. Lander

Faculty Publications

The C-C chemokine receptor 5, 32 base-pair deletion (CCR5-Δ32) allele confers strong resistance to infection by the AIDS virus HIV. Previous studies have suggested that CCR5-Δ32 arose within the past 1,000 y and rose to its present high frequency (5%-14%) in Europe as a result of strong positive selection, perhaps by such selective agents as the bubonic plague or smallpox during the Middle Ages. This hypothesis was based on several lines of evidence, including the absence of the allele outside of Europe and long-range linkage disequilibrium at the locus. We reevaluated this evidence with the benefit of much denser genetic …

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A Role For Airway Remodeling During Respiratory Syncytial Virus Infection, David Becnel, Dahui You, Joshua Erskin, Dawn M. Dimina, Stephania A. Cormier Oct 2005

A Role For Airway Remodeling During Respiratory Syncytial Virus Infection, David Becnel, Dahui You, Joshua Erskin, Dawn M. Dimina, Stephania A. Cormier

Faculty Publications

Background: Severe respiratory syncytial virus infection (RSV) during infancy has been shown to be a major risk factor for the development of subsequent wheeze. However, the reasons for this link remain unclear. The objective of this research was to determine the consequences of early exposure to RSV and allergen in the development of subsequent airway hyperreactivity (AHR) using a developmental time point in the mouse that parallels that of the human neonate. Methods: Weanling mice were sensitized and challeged with ovalbumin (Ova) and/or infected with RSV. Eight days after the last allergen challenge, various pathophysiological endpoints were examined. Results: AHR …

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Louisiana Agriculture Fall, 2005, Lsu Agcenter Oct 2005

Louisiana Agriculture Fall, 2005, Lsu Agcenter

Louisiana Agriculture

No abstract provided.

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Fuzzifying [Sic] Markov Decision Process, Ahmed Syed Irshad Jan 2005

Fuzzifying [Sic] Markov Decision Process, Ahmed Syed Irshad

LSU Master's Theses

Markov decision processes have become an indispensable tool in applications as diverse as equipment maintenance, manufacturing systems, inventory control, queuing networks and investment analysis. Typically we have a controlled Markov chain on a suitable state space in which transitional probabilities depend on the policy (or decision maker) which comes from a set of possible actions. The main problem of interest would be to find an optimal policy that minimizes the associated cost. Linear Programming has been widely used to find the optimal Markov decision policy. It requires solutions of large systems of simultaneous linear equations. By the fact that the …

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Dna Supercoiling With A Twist, Edwin Kamau Jan 2005

Dna Supercoiling With A Twist, Edwin Kamau

LSU Doctoral Dissertations

The level of torsion in double-stranded DNA regulates base-pair stability and DNA conformation. It is important in initiation and regulation of specific DNA metabolic processes as well as chromatin assembly. High mobility group proteins (HMGB) are architectural proteins whose HMG DNA binding domains confer significant preference for distorted DNA, such as supercoiled DNA and 4-way junctions. HMGB proteins play a role in transiently regulating or conserving DNA torsion. Topoisomerases regulate DNA supercoiling, which has been argued to provide a coherent explanation for the main modes of transcriptional control - stringent control, growth-rate control and growth-phase control during the normal cell …

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Health And Growth, Petia Stoianova Stoytcheva Jan 2005

Health And Growth, Petia Stoianova Stoytcheva

LSU Doctoral Dissertations

Inarguably the most important question is about the unequal distribution of income among countries. Development economists have recently turned to health for an answer. This dissertation investigates the e®ect of health on cross-country income. The first essay sheds new light on the impact of AIDS on cross-country income levels. We control for a variety of factors that are potentially related to income as suggested by our empirical model and existing related literature. Using the extended (for human capital) Solow model as our baseline empirical specification, we consider cross-sectional and panel estimation. For the full sample it is shown that AIDS …

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Characterizing The Health Status Of The Louisiana Gopher Tortoise (Gopherus Polyphemus), Orlando Diaz-Figueroa Jan 2005

Characterizing The Health Status Of The Louisiana Gopher Tortoise (Gopherus Polyphemus), Orlando Diaz-Figueroa

LSU Master's Theses

Gopher tortoise (Gopherus polyphemus) populations have experienced precipitous declines from habitat loss, and human and disease related mortality. The goal of this study was to characterize the health status of free-ranging Louisiana gopher tortoises. Gopher tortoises were collected during two distinct trapping seasons: fall (August-October 2002) and spring (April-June 2003). Captured tortoises were given a physical exam and the carapace and plastron length and width, weight, and body temperature were recorded. Blood was collected from the subcarapacial vein and submitted for the following testing procedures: complete blood count, plasma chemistry, infectious disease serology (Mycoplasma), and toxicologic screen (copper, mercury, zinc, …

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Seasonal Abundance And Detection Of West Nile Virus In Ceratopogonids (Diptera: Ceratopogonidae) In East Baton Rouge Parish, Louisiana, Isidra Joselina Sabio Jan 2005

Seasonal Abundance And Detection Of West Nile Virus In Ceratopogonids (Diptera: Ceratopogonidae) In East Baton Rouge Parish, Louisiana, Isidra Joselina Sabio

LSU Master's Theses

The seasonal abundance and species composition of ceratopogonids (Diptera: Ceratopogonidae) in East Baton Rouge parish was described from light trap collections at 15 sites. A total of 4,968 collections were processed, and 48,667 ceratopogonids were collected from 20 November, 2002 through 25 November, 2004. Three genera of ceratopogonids (Forcipomyia Meigen, Atrichopogon Kieffer, and Culicoides Latrielle) and a total of 18 species of the genus Culicoides were identified. Ceratopogonids had distinctive spring and fall population peaks. Ceratopogonids were collected in every month during the study, with the exception of February 2004. These results suggest that certain species of Culicoides may …

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Analysis Of The Talus And Calcaneus Bones From The Poole-Rose Ossuary: A Late Woodland Burial Site In Ontario, Canada, Adrienne Elizabeth Penney Jan 2005

Analysis Of The Talus And Calcaneus Bones From The Poole-Rose Ossuary: A Late Woodland Burial Site In Ontario, Canada, Adrienne Elizabeth Penney

LSU Master's Theses

This study reports on the demography and osteological profile of the Poole-Rose Ossuary. Excavated in 1990 under the direction of Heather McKillop and at the request of the Alderville First Nation, the Poole-Rose Ossuary is a Late Woodland burial site in southern Ontario, Canada. Lack of European artifacts in the burial suggests that this site predates European contact. The Poole-Rose Ossuary is radiocarbon dated to A.D. 1550 ± 50 years. The skeletal remains were commingled. This ossuary likely represents the mass re-burial known as the “Feast of the Dead” or the “Kettle.” For the most part, closely related individuals were …

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Ecotoxicology Of Metal-Hydrocarbon Mixtures In Benthic Invertebrates, Kurt A. Gust Jan 2005

Ecotoxicology Of Metal-Hydrocarbon Mixtures In Benthic Invertebrates, Kurt A. Gust

LSU Doctoral Dissertations

Metal-hydrocarbon mixtures are becoming increasingly prevalent in natural environments due to expanding industrial activity and urbanization. The ecotoxicology of metal-hydrocarbon mixtures in benthic environments is of particular concern because both classes of contaminants partition to sediments and can thereby exert toxic effects in benthic organisms. Mixtures of dissimilar chemicals (including metals and hydrocarbons) are broadly hypothesized to elicit independent toxic effects however; this hypothesis has little supporting data. The purpose of this dissertation was to test this hypothesis for metal-hydrocarbon mixtures using environmentally relevant exposures and to determine mechanisms for observed interactive effects. Sediment and water-only bioassays were conducted employing …

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Control Of Vibrio Vulnificus And Vibrio Parahaemolyticus In Oysters, Ligia Virginia Antonia Da Silva Jan 2005

Control Of Vibrio Vulnificus And Vibrio Parahaemolyticus In Oysters, Ligia Virginia Antonia Da Silva

LSU Doctoral Dissertations

Vibrio vulnificus and Vibrio parahaemolyticus are gram-negative halophilic bacteria found in the natural aquatic environment. V. vulnifcus and V. parahaemolyticus have been implicated in foodborne illness and can cause gastroenteritis that has been associated with consumption of raw or undercooked seafood. V. vulnificus can cause primary septicemia after its ingestion, and secondary septicemia through skin lesions in individuals with preexisting conditions such as elevated serum iron levels. Bacteriophages, viruses that invade and lyse bacteria, specific to V. vulnificus and V. parahaemolyticus are naturally found in seawater and oysters. Every summer, the oyster industry is threatened by recall of oysters due …

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Patient Satisfaction With Nurse Practitioner Delivered Primary Health Care Services, Lucie J. Agosta Jan 2005

Patient Satisfaction With Nurse Practitioner Delivered Primary Health Care Services, Lucie J. Agosta

LSU Doctoral Dissertations

The purpose of this study was to explore and determine the degree of client satisfaction with utilization of primary healthcare services delivered by a nurse practitioner in the Employee Health Services department of a not for profit hospital in the Southern United States. The Nurse Practitioner Satisfaction Survey (NPSS), a 28-item Likert-type survey instrument was specifically developed for this study and administered to a sample of 300 clients. Overall high levels of patient satisfaction with nurse practitioner delivered health care services were demonstrated. The mean general satisfaction score was determined to be 86.86 / 90, with mean communication and scheduling …

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Development And Characterization Of Antimicrobial Edible Films From Crawfish Chitosan, Kandasamy Nadarajah Jan 2005

Development And Characterization Of Antimicrobial Edible Films From Crawfish Chitosan, Kandasamy Nadarajah

LSU Doctoral Dissertations

Inherent antibacterial/antifungal properties and film-forming ability of chitosan make it ideal for use as a biodegradable antimicrobial packaging material. This study was attempted to develop antimicrobial films from crawfish chitosan. Traditional chitosan production involves: deproteinization (DP), demineralization (DM), decolorization (DC), and deacetylation (DA). Modification of chitosan production affects film properties. Effects of chitosan production protocols, film-casting solvents, and plasticizer contents on physicochemical, mechanical and antibacterial properties were investigated. Four chitosans were prepared from traditional (DPMCA) and modified processes [excluding either DP, DC or both DP and DC]. Chitosan (1%w/v) was dissolved in 1% acetic, ascorbic, citric, formic, lactic and/or malic …

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The Psychosocial Vulnerability Model Of Hostility As A Predictor Of Coronary Heart Disease, Karen Grothe Jan 2005

The Psychosocial Vulnerability Model Of Hostility As A Predictor Of Coronary Heart Disease, Karen Grothe

LSU Doctoral Dissertations

Coronary heart disease (CHD) is the number one killer for both men and women in the United States today. African Americans are particularly at risk, due to higher prevalence rates and mortality related to CHD (American Heart Association, 2003). Less than half of the new cases of CHD can be predicted with known risk factors (tobacco use, obesity, hypertension), which suggests the possible influence of personality or additional behavioral factors. Hostility is a psychological variable that has been consistently related to health outcomes, particularly to CHD. While the exact mechanism linking hostility to health is currently unknown, a psychosocial vulnerability …

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Urban Yellow Fever Diffusion Patterns And The Role Of Micro-Environmental Factors In Disease Dissemination: A Temporal-Spatial Analysis Of The Memphis Epidemic Of 1878, William Brennan Arden Jan 2005

Urban Yellow Fever Diffusion Patterns And The Role Of Micro-Environmental Factors In Disease Dissemination: A Temporal-Spatial Analysis Of The Memphis Epidemic Of 1878, William Brennan Arden

LSU Master's Theses

Most investigations that examine diffusion of disease through urban areas give relatively scant attention to the 'micro-environment.' Employing the many methods of macro-environmental analysis, most studies do not attempt to identify street-level or even residence-level temporal-spatial patterns of disease diffusion. As a result, the mechanisms that lead to diffusion on such small scales are left relatively undefined. There is no doubt that cumulatively these small-scale mechanisms contribute to the diffusion of disease over the urban environment. However, the level of contribution and the specific details of their dynamics remain unclear. This thesis investigated some of these unexplored areas by presenting …

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Sacred Trust: The Voluntary Removal And Reburial Of Human Remains From A Historic Cemetery In Louisiana, Christian Williamson Jan 2005

Sacred Trust: The Voluntary Removal And Reburial Of Human Remains From A Historic Cemetery In Louisiana, Christian Williamson

LSU Master's Theses

Members of the Randolph family, a prominent plantation family that has lived in Louisiana since the late eighteenth century, contacted a team of anthropologists from Louisiana State University to help recover thirteen individuals from St. Mary’s Cemetery in Bayou Goula, Louisiana. The cemetery had been abandoned since 1970, its graves overgrown with weeds and desecrated by vandals. Of the thirteen individuals recovered, three sets of remains did not have associated grave markers. These three were taken back to the lab and analyzed using standard forensic procedures. Documentary research on the history of the cemetery, the once associated church, and the …

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Genetic Analysis Of The Herpes Simplex Virus Type 1 Ul20 Protein Domains Involved In Cytoplasmic Virion Envelopment And Virus-Induced Cell Fusion, Jeffrey M. Melancon, Timothy P. Foster, Konstantin G. Kousoulas Jul 2004

Genetic Analysis Of The Herpes Simplex Virus Type 1 Ul20 Protein Domains Involved In Cytoplasmic Virion Envelopment And Virus-Induced Cell Fusion, Jeffrey M. Melancon, Timothy P. Foster, Konstantin G. Kousoulas

Faculty Publications

The herpes simplex virus type 1 UL20 protein (UL20p) is an important determinant for cytoplasmic virion morphogenesis and virus-induced cell fusion. To delineate the functional domains of the UL20 protein, we generated a panel of single and multiple (cluster) alanine substitutions as well as UL20p carboxyl-terminal truncations. The UL20 mutant genes could be broadly categorized into four main groups: Group I UL20 mutant genes complemented for both virus production and virus-induced cell fusion; Group II UL20 mutant genes did not complement for either virus-induced cell fusion or infectious virus production; Group II UL20 mutant genes complemented for virus-induced cell fusion …

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The Herpes Simplex Virus Type 1 Ul20 Protein Modulates Membrane Fusion Events During Cytoplasmic Virion Morphogenesis And Virus-Induced Cell Fusion, Timothy P. Foster, Jeffrey M. Melancon, Joel D. Baines, Konstantin G. Kousoulas May 2004

The Herpes Simplex Virus Type 1 Ul20 Protein Modulates Membrane Fusion Events During Cytoplasmic Virion Morphogenesis And Virus-Induced Cell Fusion, Timothy P. Foster, Jeffrey M. Melancon, Joel D. Baines, Konstantin G. Kousoulas

Faculty Publications

The herpes simplex virus type 1 (HSV-1) UL20 protein is an important determinant for virion morphogenesis and virus-induced cell fusion. A precise deletion of the UL20 gene in the HSV-1 KOS strain was constructed without affecting the adjacent UL20.5 gene. The resultant KOS/UL20-null virus produced small plaques of 8 to 15 cells in Vero cells while it produced wild-type plaques on the complementing cell line G5. Electron microscopic examination of infected cells revealed that the KOS/UL20-null virions predominantly accumulated capsids in the cytoplasm while a small percentage of virions were found as enveloped virions within cytoplasmic vacuoles. Recently, it was …

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Assessing The Identity Of Black Indians In Louisiana: A Quantitative And Qualitative Analysis, Francis J. Powell Jan 2004

Assessing The Identity Of Black Indians In Louisiana: A Quantitative And Qualitative Analysis, Francis J. Powell

LSU Doctoral Dissertations

This study shows the existence of Black Indians in Louisiana and investigates whether differences exist between Black Indians who are members of officially recognized tribes and those who do not have any type of recognition. The study examined if a relationship exist between tribal recognition and ethnic identity, subjective well-being, and social support. A cross-sectional survey design was used. Semi-structured interviews were conducted to obtain qualitative data. The sample consisted of 60 participants. 30 were from recognized tribal groups and 30 were from non-recognized tribal communities. The study specifically examined variables related to the perceptions of Black Indians in Louisiana …

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A Spatial Analysis Of The Smallpox Epidemic In Sheffield, United Kingdom, 1887-1888, Ann-Marie Cain Jan 2004

A Spatial Analysis Of The Smallpox Epidemic In Sheffield, United Kingdom, 1887-1888, Ann-Marie Cain

LSU Master's Theses

One of today's most fecund fields of research in medical geography involves using historical datasets from past epidemics and contemporary cadastre maps to plot the course of the contagion. The purpose is to study the spatial patterns of diseases that are rare or extinct today but still have large research value. The best example of this problem is smallpox. This project involves taking one of the most complete records of a smallpox epidemic, plotting the cases in a geographic information system (GIS), and exploring the spatial patterns using statistical software. As one of the most contagious and damaging diseases to …

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The Control, Survival, And Growth Of Listeria Monocytogenes On Food Products, Richelle Lynn Beverly Jan 2004

The Control, Survival, And Growth Of Listeria Monocytogenes On Food Products, Richelle Lynn Beverly

LSU Doctoral Dissertations

Listeria monocytogenes, a ubiquitous foodborne pathogen, was recognized over 70 years ago. It is the source of the human disease listeriosis. The majority of Listeria monocytogenes that have been isolated from food product or human cases are of the serotypes ½ a, ½ b and 4b. Due to the recent outbreaks, recalls and deaths associated with Listeria monocytogenes in ready-to-eat meat products, the United States Department of Agriculture (USDA), Food Safety and Inspection Service (FSIS) on October 2, 2003 issued a directive for the control of Listeria monocytogenes on ready-to-eat products. The ready-to-eat food industry must impose a post-lethality treatment …

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Chromosomal Localization Of A Proinsulin Transgene Inserted With A Transposon-Based Vector Into Japanese Quail, Coturnix Coturnix, Lacey R. Mcnally Jan 2004

Chromosomal Localization Of A Proinsulin Transgene Inserted With A Transposon-Based Vector Into Japanese Quail, Coturnix Coturnix, Lacey R. Mcnally

LSU Doctoral Dissertations

The overall goals of this research were to develop a reproducible method of detecting stable DNA insertion into Japanese quail and provide a method for gene location on avian chromosomes. This research resulted in the development of a different method of obtaining chromosome spreads in Japanese quail, the establishment of primed in situ hybridization as a method for the chromosomal gene detection in birds, development of Teflon-coated coverslip slides to facilitate laser microdissection of 0.5 ƒÝm samples, and chromosomal identification of proinsulin transgene insertions by laser microdissection and nucleotide sequence from G2 Japanese quail. The 28S rDNA was found on …

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Estimation Of The Effect Of Misclassifications On Diagnostic Test Performance In Two Persistent Bovine Viral Infections, Kimberly Orr Jan 2004

Estimation Of The Effect Of Misclassifications On Diagnostic Test Performance In Two Persistent Bovine Viral Infections, Kimberly Orr

LSU Doctoral Dissertations

Validation of diagnostic assay performance is hampered where no gold standard exists. Bayesian estimates of the sensitivity and specificity were applied to studies of two persistent bovine infections: bovine immunodeficiency virus (BIV), and bovine viral diarrhea virus (BVDV). BVDV pathogenesis is well described. Losses of $20 million in 1992 alone led the Danish government to mandate eradication. Two enzyme-linked immunosorbent assay (ELISA) tests were developed; one antibody-based to detect exposure, one antigen-based to detect persistent infection. Bayesian estimates of the sensitivity and specificity were 96.6% and 99.2%, and 98.2% and 99.8% for the antibody and antigen ELISA, respectively. Maximum Likelihood …

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Photo-Control Of Dna Oligonucleotides With Cage Compounds, Bilal Ghosn Jan 2004

Photo-Control Of Dna Oligonucleotides With Cage Compounds, Bilal Ghosn

LSU Master's Theses

Many biochemical processes in which DNA and other nucleic acids participate are central to functions in both living cells and in molecular biology assays. While many compounds have been used to regulate the activity of DNA, these strategies are limited to the aqueous-based diffusion of the activator to the target DNA molecule. An improvement to the induction of DNA bioactivity is to move to a light-based modulation. This research demonstrates a light-based technique using a photo-cleavable cage compound to transiently inactivate DNA hybridization. Function can be restored with exposure to near-UV light, allowing for temporal control of DNA oligonucleotide (ODN) …

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Overexpression Of Gk In Gk-Transformed Cells Collapses The Golgi Apparatus Into The Endoplasmic Reticulum Inhibiting Virion Egress, Glycoprotein Transport, And Virus-Induced Cell Fusion, Timothy P. Foster, Galena V. Rybachuk, Xavier Alvarez, Olga Borkhsenious, Konstantin G. Kousoulas Dec 2003

Overexpression Of Gk In Gk-Transformed Cells Collapses The Golgi Apparatus Into The Endoplasmic Reticulum Inhibiting Virion Egress, Glycoprotein Transport, And Virus-Induced Cell Fusion, Timothy P. Foster, Galena V. Rybachuk, Xavier Alvarez, Olga Borkhsenious, Konstantin G. Kousoulas

Faculty Publications

Intracellular transport and egress of alphaherpesviruses require the coordinate function of multiple proteins and glycoproteins. Recently, we showed that gK is expressed on infected cell surfaces and that gK cell-surface expression required the presence of the UL20 protein [J. Virol. 77 (2003), 499]. Overexpression of gK by gK-transformed cells blocked transport of enveloped virions from perinuclear spaces and inhibited virus-induced cell fusion caused by gK syncytial mutants [J. Virol. 69 (1995), 5401]. Therefore, we investigated whether altered synthesis and transport of gK was responsible for the observed gK-mediated interference phenomena. HSV-1 infection of the gK-transformed cell line Vero (gK9) caused …

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Louisiana Agriculture Fall, 2003, Lsu Agcenter Oct 2003

Louisiana Agriculture Fall, 2003, Lsu Agcenter

Louisiana Agriculture

No abstract provided.

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The Effect Of Density-Dependent Catastrophes On Population Persistence Time, Chris Wilcox, Bret Elderd Oct 2003

The Effect Of Density-Dependent Catastrophes On Population Persistence Time, Chris Wilcox, Bret Elderd

Faculty Publications

1. There has been increasing recognition that catastrophes are an important factor in modelling threatened populations. However, density dependence has generally been omitted from models of threatened populations on the assumption that this omission yields conservative predictions. We explore the significance of including density-dependent catastrophes in models of threatened populations. 2. Using an analytical model, we show that density-dependent catastrophes have a significant effect on population persistence, decreasing mean persistence time at large population sizes and causing a relative increase at intermediate sizes. 3. We illustrate our results with empirical data from a disease outbreak in crabeater seals Lobodon carcinophagus …

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Maxizyme-Mediated Specific Inhibition On Mutant-Type P53 In Vitro, Xin Juan Kong, Yu Hu Song, Ju Sheng Lin, Huan Jun Huang, Nan Xia Wang, Nan Zhi Liu, Bin Li, You Xin Jin Jul 2003

Maxizyme-Mediated Specific Inhibition On Mutant-Type P53 In Vitro, Xin Juan Kong, Yu Hu Song, Ju Sheng Lin, Huan Jun Huang, Nan Xia Wang, Nan Zhi Liu, Bin Li, You Xin Jin

Faculty Publications

Aim: To evaluate the specific inhibition of maxizyme directing against mutant-type p53 gene (mtp53) at codon 249 in exon 7 (AGG→AGT) in vitro. Methods: Two different monomers of anti-mtp53 maxizyme (maxizyme right MzR, maxizyme left MzL) and control mutant maxizyme (G5→A5) were designed by computer and cloned into vector pBSKU6 (pBSKU6MzR, pBSKU6MzL). After being sequenced, the restrictive endonuclease site in pBSKU6MzR was changed by PCR and then U6MzR was inserted into pBSKU6MzL, the recombinant vector was named pU6Mz and pU6asMz (mutant maxizyme). Mtp53 and wild-type p53 (wtp53) gene fragments were cloned into pGEM-T vector under the T7 promoter control. The …

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