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Effects Of Metformin On Glucose Metabolism And Mitochondrial Function In Patients With Obstructive Sleep Apnea: A Pilot Randomized Trial, Elizabeth R.M. Zunica, Elizabeth C. Heintz, Wagner S. Dantas, R. Caitlin Hebert, Ma Kayla Tanksley, Robbie A. Beyl, Edward C. Mader, John P. Kirwan, Christopher L. Axelrod, Prachi Singh

Division of Basic Science Faculty Publications

Obstructive sleep apnea (OSA) is associated with increased risk for diabetes, and standard treatment with positive airway pressure (PAP) device shows inconsistent effects on glucose metabolism. Metformin is known to treat and prevent diabetes, but its effects on skeletal muscle mitochondrial function are not completely understood. Here, we evaluate the effects of metformin on glucose metabolism and skeletal muscle mitochondrial function in patients with OSA. Sixteen adults with obesity (50.9 ± 6.7 years, BMI: 36.5 ± 2.9 kg/m2) and moderate-to-severe OSA were provided with PAP treatment and randomized to 3 months of placebo (n = 8) or metformin (n = …

Embryonic Exposure To The Benzotriazole Ultraviolet Stabilizer 2-(2h-Benzotriazol-2-Yl)-4-Methylphenol Decreases Fertility Of Adult Zebrafish (Danio Rerio), Yamin Raza, Emily Mertens, Lauren Zink, Zhe Lu, Jon A. Doering, Steve Wiseman

Faculty Publications

Benzotriazole ultraviolet stabilizers (BUVSs) are emerging contaminants of concern. They are added to a variety of products, including building materials, personal care products, paints, and plastics, to prevent degradation caused by ultraviolet (UV) light. Despite widespread occurrence in aquatic environments, little is known regarding the effects of BUVSs on aquatic organisms. The aim of the present study was to characterize the effects of exposure to 2-(2H-benzotriazol-2-yl)-4-methylphenol (UV-P) on the reproductive success of zebrafish (Danio rerio) following embryonic exposure. Embryos were exposed, by use of microinjection, to UV-P at < 1.5 (control), 2.77, and 24.25 ng/g egg, and reared until sexual maturity, when reproductive performance was assessed, following which molecular and biochemical endpoints were analyzed. Exposure to UV-P did not have a significant effect on fecundity. However, there was a significant effect on fertilization success. Using UV-P-exposed males and females, fertility was decreased by 8.75% in the low treatment group and by 15.02% in the high treatment group relative to control. In a reproduction assay with UV-P-exposed males and control females, fertility was decreased by 11.47% in the high treatment group relative to the control. Embryonic exposure to UV-P might have perturbed male sex steroid synthesis as indicated by small changes in blood plasma concentrations of 17β-estradiol and 11-ketotestosterone, and small statistically nonsignificant decreases in mRNA abundances of cyp19a1a, cyp11c1, and hsd17b3. In addition, decreased transcript abundances of genes involved in spermatogenesis, such as nanos2 and dazl, were observed. Decreases in later stages of sperm development were observed, suggesting that embryonic exposure to UV-P impaired spematogenesis, resulting in decreased sperm quantity. The present study is the first to demonstrate latent effects of BUVSs, specifically on fish reproduction. Environ Toxicol Chem 2024;43:385–397. © 2023 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.

Opposing Genetic Patterns Of Range Shifting Temperate And Tropical Gastropods In An Area Undergoing Tropicalisation, Karolina M. Zarzyczny, Michael E. Hellberg, Elena B. Lugli, Moira Maclean, David A. Paz-García, Marc Rius, Ethan G. Ross, Erick X. Treviño Balandra, James Vanstone, Suzanne T. Williams, Phillip B. Fenberg

Faculty Publications

Aim: The poleward range expansion of tropical species, and range contraction of temperate species (known as tropicalisation) has mainly been studied from an ecological perspective, with little research on its genetic consequences. Here, we used distributional and genetic data to document the consequences of tropicalisation in rocky shore gastropods and assess more broadly the future implications of tropicalisation on phylogeographic patterns. Location: Nineteen sampling sites along >3000 km of the eastern Pacific rocky intertidal zone, from the tip of the Baja California Peninsula to southern California. Taxon: Temperate gastropods: Lottia conus, L. strigatella, Fissurella volcano and Tegula gallina. Tropical gastropods: …

Simultaneous Detection And Quantification Of Multiple Pathogen Targets In Wastewater, Gouthami Rao, Drew Capone, Kevin Zhu, Abigail Knoble, Yarrow Linden, Ryan Clark, Amanda Lai, Juhee Kim, Ching Hua Huang, Aaron Bivins, Joe Brown

Faculty Publications

Wastewater-based epidemiology has emerged as a critical tool for public health surveillance, building on decades of environmental surveillance work for pathogens such as poliovirus. Work to date has been limited to monitoring a single pathogen or small numbers of pathogens in targeted studies; however, few studies consider simultaneous quantitative analysis of a wide variety of pathogens, which could greatly increase the utility of wastewater surveillance. We developed a novel quantitative multi-pathogen surveillance approach (35 pathogen targets including bacteria, viruses, protozoa, and helminths) using TaqMan Array Cards (TAC) and applied the method on concentrated wastewater samples collected at four wastewater treatment …

Energy And Spectrum Efficient Federated Learning Via High-Precision Over-The-Air Computation, Liang Li, Chenpei Huang, Dian Shi, Hao Wang, Xiangwei Zhou, Minglei Shu, Miao Pan

Faculty Publications

Federated learning (FL) enables mobile devices to collaboratively learn a shared prediction model while keeping data locally. However, there are two major research challenges to practically deploy FL over mobile devices: (i) frequent wireless updates of huge size gradients v.s. limited spectrum resources, and (ii) energy-hungry FL communication and local computing during training v.s. battery-constrained mobile devices. To address those challenges, in this paper, we propose a novel multi-bit over-the-air computation (M-AirComp) approach for spectrum-efficient aggregation of local model updates in FL and further present an energy-efficient FL design for mobile devices. Specifically, a high-precision digital modulation scheme is designed …

Parental Environnement Influences Offspring Gene Expression In The Olympia Oyster, Ostrea Lurida., Mark S. Yeats

LSU Master's Theses

The Olympia Oyster is the only native oyster species on the Pacific coast of North America. Seasonality in water temperatures across this threatened species’ broad and fragmented range has been linked to its development, reproductive timing, reproductive activity, growth, and survival rates. Recent evidence suggests parental environments can have carry-over effects on larval growth and respiration rates in response to temperature. In this study, we utilized transcriptomic methods to identify the underlying mechanisms of this cross-generation plasticity. Sequence libraries of adult ctenidia tissue and pooled larval replicates were extracted and spawned from hatchery raised oysters of the same parentage immediately …

In Search Of The Armorial Aesthetic: The Counter-Reform Of Ariano Suassuna And The Compositional Techniques In Clovis Pereira’S Suite Macambira, Wagner De Oliveira Duarte

LSU Doctoral Dissertations

Throughout the first half of the twentieth century, in the wake of the Week of Modern Art in São Paulo in 1922, Brazil grappled with defining its national identity, distinct from European influences. While eminent figures such as Villa-Lobos, Camargo Guarnieri, and Francisco Mignone provided foundational narratives in this quest, the Armorial movement emerged as a unique and emblematic response to these nationalistic aspirations.

Originating from the creative vision of Ariano Suassuna, the Armorial movement, though often perceived as a regional initiative, epitomized a broader endeavor to represent Brazil's diverse cultural tapestry. Central to understanding this movement is the work …

Death, Dreaming, And Diaspora: Achieving Orientation Through Afro-Spirituality, Liz Johnston, Jaime Elizabeth Johnston

LSU Doctoral Dissertations

Enslavement, colonization, and the systems that uphold racial injustice were and still are a series of new, unfathomable, and challenging experiences that prompt individuals within the diaspora to seek orientation. How does a human cope with centuries of attempts at the systematic destruction of their humanity, culture, and identity? How can they reclaim that identity, especially when so much of it seems lost? I address these questions by utilizing texts from the expansive body of work regarding ethnographic-historical-religious studies on Afro-spiritual practices to better analyze instances in literature in the ongoing practice of diasporic orientation. In this project, I argue …

Incubating Education: The Lived Experiences Of Louisiana Elementary And Middle School Teachers When Piloting A 4-H Stem School Enrichment Curriculum, Crystal Rose Ahrens

LSU Doctoral Dissertations

This study addressed a deficiency in the agricultural literacy of the U.S. population by focusing on the challenges that core subject teachers face when integrating agriculture into their curriculum. Using a phenomenological research approach, this investigation explored the lived experiences of Louisiana teachers who piloted a 4-H STEM curriculum, focusing on the growth in agricultural literacy of the teachers while instructing third and eighth-grade students on the agricultural-based and STEM-focused topic of embryology. Employing a transcendental phenomenological approach, I acknowledged my biases through reflexivity and adhered to ethical considerations, including informed consent and confidentiality. Key findings emerged through four themes …

Time, Place, & Purpose: The Performance Of Creole Identity In Louisiana, Rachel N. Aker

LSU Doctoral Dissertations

Though much of the early development of Louisiana Creole culture can be found in New Orleans, the culture spread and continued to grow throughout the rest of South Louisiana in both similar and different ways. Expanding beyond Joseph Roach’s treatment of Creole cultural performances in New Orleans in Cities of the Dead (1996) and journeying across land and water, this project identifies more Creole cultural performance as they emerge across place and time. I present Louisiana and the Gulf South as a kind of inland archipelago, with the currents of culture-creation moving in and around distinct community enclaves. The flow …

Soil Improvement Using Fungal Mycelium Of Trichoderma Virens And Construction Techniques Of Mud Dauber, Park Joon Soo

LSU Doctoral Dissertations

Traditional ground improvement techniques involve high energy consumption and expensive and invasive earthworks. To develop sustainable ground improvement techniques, extensive research has explored biological processes and strategies. This dissertation identifies two organisms, fungal mycelium and mud dauber wasp, as sources of utilization and inspiration for the development of sustainable soil improvement techniques.

Filamentous fungi can form a root-like interconnected complex network known as fungal mycelium. Fungal mycelium can extend through soil pores and secrete hydrophobic compounds, binding soil particles and increasing soil water repellency. This dissertation conducted experimental studies to investigate the effects of fungal mycelium on hydraulic and mechanical …

Habitat Use And Movements Of Parrotfishes In A Hawaiian Coral Reef Seascape, Senifa F. Annandale, Jason P. Turner, Daniel L. Lippi, Michael Dance, R. J.David Wells, Jay R. Rooker

Faculty Publications

Parrotfishes (family Scaridae) are important to coral reef ecosystems and highly prized by both commercial and recreational fishers in the Hawaiian Islands. Although parrotfishes are known to be strictly diurnal, our knowledge of their habitat use and movement patterns across daily timescales in tropical systems is still somewhat limited. Here, acoustic telemetry was used to determine parrotfish habitat use and movements within a coral reef seascape at Puakō, Hawai‘i. An array of acoustic receivers was deployed in a nearshore reef to track fine-scale movements of two common species: ember parrotfish (Scarus rubroviolaceus) and palenose parrotfish (Scarus psittacus). Transmitters were mounted …

Seaweed As A Resilient Food Solution After A Nuclear War, Florian Ulrich Jehn, Farrah Jasmine Dingal, Aron Mill, Cheryl Harrison, Ekaterina Ilin, Michael Y. Roleda, Scott C. James, David Denkenberger

Faculty Publications

Abrupt sunlight reduction scenarios such as a nuclear winter caused by the burning of cities in a nuclear war, an asteroid/comet impact or an eruption of a large volcano inject large amounts of particles in the atmosphere, which limit sunlight. This could decimate agriculture as it is practiced today. We therefore need resilient food sources for such an event. One promising candidate is seaweed, as it can grow quickly in a wide range of environmental conditions. To explore the feasibility of seaweed after nuclear war, we simulate the growth of seaweed on a global scale using an empirical model based …

Serine Proteinase Inhibitors From Nicotiana Benthamiana, A Nonpreferred Host Plant, Inhibit The Growth Of Myzus Persicae (Green Peach Aphid), Honglin Feng, Georg Jander

Faculty Publications

BACKGROUND: The green peach aphid (Myzus persicae) is a severe agricultural crop pest that has developed resistance to most current control methods, requiring the urgent development of novel strategies. Plant proteinase inhibitors (PINs) are small proteins that protect plants against pathogens and/or herbivores, likely by preventing efficient protein digestion. RESULTS: We identified 67 protease genes in the transcriptomes of three M. persicae lineages (USDA-Red, G002 and G006). Comparison of gene expression levels in aphid guts and whole aphids showed that several proteases, including a highly expressed serine protease, are significantly overexpressed in the guts. Furthermore, we identified three genes encoding …

Diving At High Altitude: O2 Transport And Utilization In The Ruddy Duck And Torrent Duck In The Andes, Kevin G. Mccracken, Graham R. Scott, Luis Alza, Andrea Astie, Ciska Bakkeren, Emil Bautista, Mariana Bulgarella, Rebecca Cheek G. Cheek, Beverly A. Chua, Neal J. Dawson, Alexis Diaz, Catherine M. Ivy, Peter B. Frappell, Cecilia Kopuchian, Sabine L. Laguë, John N. Maina, Violeta Muñoz-Fuentes, Elizabeth R. Schell, Matthew M. Smith, Ryan J. Sprenger, Pablo L. Tubaro, Elizabeth R. Schell, Matthew M. Smith, Ryan J. Sprenger, Pablo L. Tubaro, Thomas Valqui, Roy E. Weber, Daniela Wilner, Robert E. Wilson, Julia M. York, William K. Milsom

Occasional Papers of the Museum of Natural Science, Louisiana State University

Hypoxia and cold temperatures create unique physiological challenges for high-altitude organisms that can vary depending on lifestyle. While nearly all studies of air-breathing animals at high altitude are from terrestrial species, species that breath-hold dive underwater at high altitude encounter a very different set of selective pressures influencing their phenotype. The goal of this publication is to highlight the changes in O2 transport and utilization in high-altitude diving birds relative to divers at sea level, and the extent to which these changes are qualitatively distinct from phenotypic changes in non-diving species at high altitude. For example, while high capacities for …

Louisiana Agriculture Magazine, Winter 2024, Lsu Agcenter

Louisiana Agriculture

Research at the LSU AgCenter extends well beyond the farm gate. Across 150 acres at the Hammond Research Station, experts in landscape horticulture test the best ways to grow ornamental plants and trees. In Baton Rouge, researchers study how to protect homes in the path of a hurricane. Across the state the AgCenter Healthy Communities team works to empower food pantries and improve sidewalks and other pedestrian infrastructure in rural areas.

This issue of Louisiana Agriculture focuses on these topics along with new research on soil health and high-tech delivery systems for sugarcane ripening agents. Other articles focus on the …

Captivity’S Collections: Science, Natural History, And The British Transatlantic Slave Trade, Kennon H. Keiser Jr.

Civil War Book Review

Grounded in extensive archival research on both sides of the Atlantic, Captivity's Collections mines scientific treatises, slaving companies' records, naturalists' correspondence, and museum catalogs to recover in rich detail the scope of the slave trade's collecting operations. The book reveals the scientific and natural historical profit derived from these activities and the crucial role of specimens gathered along the routes of the slave trade on emerging ideas in natural history.

Another Look: Animal Histories Of The Civil War Era, Henry Motty

Civil War Book Review

The chaos and suffering that swept through the United States during the Civil War affected not only humans—but also the animal world. In Animal Histories of the Civil War, a team of Civil War scholars argue how animal contributions, such as providing mobility, food, and companionship, not only impacted the way the war was fought, but also determined its outcome and the way it was remembered. Edited by Earl J. Hess, author of Civil War Logistics: A Study of Military Transportation and the former Stewart W. McClelland Chair in History at Lincoln Memorial University, Animal Histories highlights the relationship and …

Environmental History In Focus, Kennon H. Keiser Jr.

Civil War Book Review

The book reviews in this winter 2024 issue of the Civil War Book Review emphasize the role of the environment during the Civil War Era. While our two feature essays take us out of the realm of environmental history and into the world of politics, the primary focus of this winter issue is the role of nature in history. In recent years, historians have added to our knowledge of the environment’s role in the past. Nowhere is this more clear than in the Civil War Era. As historian Stephen Berry explained, “Like the Columbian Exchange, the Civil War was a …

Scars On The Land: An Environmental History Of Slavery In The American South, Rich Newman

Civil War Book Review

In Scars on the Land, David Silkenat offers an authoritative and expansive treatment of slavery’s environmental impact on the American south. Based on a wide range of sources – from slave narratives to slaveholders’ reports on nature – and a deep reading of recent scholarship on early environmental reform, the rise of capitalism, and Black resistance to bondage, the book expertly examines the ecological transformation of southern landscapes between the colonial era and the Civil War.

The Key To The Shenandoah Valley: Geography And The Civil War Struggle For Winchester, Johnathan M. Ward

Civil War Book Review

The Key to the Shenandoah Valley offers a substantial military study of the topographical and environmental features surrounding the town of Winchester, Virginia during the Civil War. Edward McCaul focuses on six specific battles for control over the town of Winchester, but he examines these military struggles in a much larger context to indicate how the geography of the Valley was crucial to Civil War strategy. In so doing, he effectively combines environmental history, military history, and political history to reveal the importance of Winchester and its terrain.

Empire Of Brutality: Enslaved People And Animals In The British Atlantic World, Owen Clow

Civil War Book Review

Empire of Brutality: Enslaved People and Animals in the British Atlantic World, Christopher Michael Blakley’s first book and a revision of their 2019 dissertation, traces how British slaveowners distinguished between people, animals, and those persons who, through enslavement, occupied a position “in-between,” and how that process of dehumanization was molded by the material human-animal relationships that structured Atlantic slavery.

Women, Animals, Food: Planetary Perspectives On The Non-(Hu)Man, Samu/Elle Striewski

Comparative Woman

The paper comparatively reads Mahasweta Devi’s Pterodactyl, Pirtha, and Puran Sahay (1995) and Margaret Atwood’s The Year of the Flood (2009) to trace the ways in which both novels show the complex intertwinement of the climate crisis with gender, class, race, subalternity, anthropocentrism, and veganism. Bringing together Gayatri C. Spivak’s notion of “planetarity” with ecofeminist philosophy and literary criticism, the article proposes a planetary ecogender reading of the two texts and their representation of the non-man, non-human, and non-subject. Building up further on Jacques Derrida’s critique of carno-phallogocentrism, the pedagogy of a relational ethics of “nurturing” is hence presented …

Establishment Of A Practical Sperm Cryopreservation Pathway For The Axolotl (Ambystoma Mexicanum): A Community-Level Approach To Germplasm Repository Development, Nicholas Coxe, Yue Liu, Lucía Arregui, Rose Upton, Sarah Bodenstein, Steven Randal Voss, Maria T. Gutierrez-Wing, Terrence R. Tiersch

Faculty Publications

The axolotl (Ambystoma mexicanum) draws great attention around the world for its importance as a biomedical research model, but housing and maintaining live animals is increasingly expensive and risky as new transgenic lines are developed. The goal of this work was to develop an initial practical pathway for sperm cryopreservation to support germplasm repository development. The present study assembled a pathway through the investigation of axolotl sperm collection by stripping, refrigerated storage in various osmotic pressures, cryopreservation in various cryoprotectants, and in vitro fertilization using thawed sperm. By the stripping of males, 25–800 µL of sperm fluid was collected at …

Transcriptomic Changes In Glomeruli In Response To A High Salt Challenge In The Dahl Ss Rat, Marharyta Semenikhina, Daria V. Lysikova, Denisha R. Spires, Mark Domondon, Krisztian Stadler, Oleg Palygin, Daria V. Ilatovskaya

Division of Basic Science Faculty Publications

Salt sensitivity impacts a significant portion of the population and is an important contributor to the development of chronic kidneydisease. One of the significant early predictors of salt-induced damage is albuminuria, which reflects the deterioration of the renal filtration barrier: the glomerulus. Despite significant research efforts, there is still a gap in knowledge regarding the molecularme chanisms and signaling networks contributing to instigating and/or perpetuating salt-induced glomerular injury. To address this gap, we used 8-wk-old male Dahl salt-sensitive rats fed a normal-salt diet (0.4% NaCl) or challenged with a high-salt diet (4%NaCl) for 3 wk. At the end of the …

Renal Epithelial Mitochondria: Implications For Hypertensive Kidney Disease, Krisztian Stadler, Daria V. Ilatovskaya

Division of Basic Science Faculty Publications

According to the Centers for Disease Control and Prevention, 1 in 2 U.S. adults have hypertension, and more than 1 in 7 chronic kidney disease. In fact, hypertension is the second leading cause of kidney failure in the United States; it is a complex disease characterized by, leading to, and caused by renal dysfunction. It is well-established that hypertensive renal damage is accompanied by mitochondrial damage and oxidative stress, which are differentially regulated and manifested along the nephron due to the diverse structure and functions of renal cells. This article provides a summary of the relevant knowledge of mitochondrial bioenergetics …

Covid-19 As A Chronic Stressor And The Importance Of Individual Identity: A Data-Driven Look At Academic Productivity During The Pandemic, Breanna N. Harris, Carolyn M. Bauer, James A. Carr, Caitlin R. Gabor, Jennifer L. Grindstaff, Caleigh Guoynes, Jennifer J. Heppner, Cris C. Ledon-Rettig, Patricia C. Lopes, Sharon E. Lynn, Carla B. Madelaire, Lorin A. Neuman-Lee, Maria G. Palacios, Paul Soto, Jennifer Terry

Faculty Publications

The COVID-19 pandemic impacted personal and professional life. For academics, research, teaching, and service tasks were upended and we all had to navigate the altered landscape. However, some individuals faced a disproportionate burden, particularly academics with minoritized identities or those who were early career, were caregivers, or had intersecting identities. As comparative endocrinologists, we determine how aspects of individual and species-level variation influence response to, recovery from, and resilience in the face of stressors. Here, we flip that framework and apply an integrative biological lens to the impact of the COVID-19 chronic stressor on our endocrine community. We address how …

Alcohol And Cannabis Dual Use Among Black Adults: Associations With Alcohol Use, Use-Related Problems, And Race-Based Discrimination, Julia D. Buckner, Michael J. Zvolensky, Caroline R. Scherzer

Faculty Publications

Background and Objectives: Black adults who drink alcohol appear at risk for poor alcohol-related outcomes, yet little research has examined whether cannabis use among those who consume alcohol (alcohol-cannabis dual use) is related to worse alcohol-related consequences, as observed in predominantly White samples. Further, it may be that experiencing more race-based discrimination may be related to using multiple substances to cope with such experiences; however, no known studies have examined the impact of race-based discrimination on alcohol-cannabis dual use. Methods: Participants were 270 Black undergraduates who endorsed past-month drinking, 112 of whom endorsed alcohol-cannabis dual use. Results: The dual use …

Male Mouse Skeletal Muscle Lacking Hur Shows Enhanced Glucose Disposal At A Young Age, Robert C. Noland, Sujoy Ghosh, Carlos J. Crisanto, Antonio Aleman, Mckenna K. Chaney, Maitri K. Chauhan, Layla G. Loftis, Ally C. Goad, Christin F. Rickman, Samuel E. Velasquez, Jaycob D. Warfel

Division of Basic Science Faculty Publications

Introduction: Metabolic flexibility is the ability of a system to switch between metabolic substrates. Human and murine skeletal muscle tissues and cells with decreased activity of the regulatory RNA-binding protein, human antigen R (HuR), have decreased capacity for fat oxidation, and thus decreased metabolic flexibility. In this study, we aimed to assess the preference for carbohydrates in mice lacking HuR in skeletal muscle. Methods: Experiments were performed on weight-matched control and HuR knockout mice of both sexes. Palmitate and pyruvate oxidation were performed in mouse muscle following the release of 14CO2. In vivo glucose and lipid uptake were assayed in …

Strategic Environmental Consumer Segmentation: An Exploratory Case Study In Slovakia, Hana Maťová, Miroslava Oravcová Triznová, Vladislav Kaputa, Erika Loučanová, Richard P. Vlosky

Faculty Publications

The desire to live in a healthy “green” environment is becoming increasingly important to global populations. The term “green” is overused, confusing, and inconsistently used among and between consumers, corporate enterprises, environmental groups, government entities, and other segments globally. Research studies suggest that defining and applying the term “green” to behaviors and decision-making is constantly changing, further complicating the study of corporate and consumer environmental responsibility. In this study, we focus on the consumer. Not all consumers behave the same or have the same beliefs and perceptions regarding what is environmentally “green.” As such, we segmented consumers in Slovakia into …