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Office Of The Vice Provost For Research Annual Report - 2010, Office Of The Vice Provost For Research, East Tennessee State University
Office Of The Vice Provost For Research Annual Report - 2010, Office Of The Vice Provost For Research, East Tennessee State University
Annual Reports
No abstract provided.
Mapping The Dorsal Skin Pigmentation Patterns Of Two Sympatric Populations Of Ambystomatid Salamanders, Ambystoma Opacum And A. Maculatum From Northeast Tennessee., Lok Raj Pokhrel
Electronic Theses and Dissertations
Because of growing concern of habitat fragmentation and its adverse effects on salamander communities in Appalachian region, sympatric populations of ambystomatid salamanders A. opacum and A. maculatum were studied in Northeast Tennessee to address a number of questions: i) the extent of sexual size dimorphism (SSD) in both species, ii) what traits influence the dorsal skin pigmentation and how, iii) whether gender differences in developmental stability occur, and iv) the extent of phenotypic variation within each species. The findings of this study revealed SSD in both species of salamanders. The most parsimonious statistical model was developed that explained the influence …
Nectin-1 Is Degraded In Chlamydia Trachomatis-Infected Genital Epithelial Cells And Is Required For Herpes Simplex Virus Co-Infection-Induced C. Trachomatis Persistence., Jingru Sun
Electronic Theses and Dissertations
The obligate intracellular bacterium Chlamydia trachomatis is the most common bacterial STD agent in the US. This bacterium has a unique biphasic developmental cycle in which the infectious elementary body (EB) infects a host mucosal epithelial cell and differentiates into the replicative form (the reticulate body or RB) within a modified vacuole called an inclusion. The RB later divides and develops back into an EB and is released, perpetuating the infectious cycle. When developing chlamydiae are exposed to unfavorable environmental conditions, they deviate from the normal developmental cycle into a non-infectious but viable state termed persistence. Previous data from our …
Office Of The Vice Provost For Research Annual Report - 2009, Office Of The Vice Provost For Research, East Tennessee State University
Office Of The Vice Provost For Research Annual Report - 2009, Office Of The Vice Provost For Research, East Tennessee State University
Annual Reports
No abstract provided.
2009-2010 - College Of Pharmacy Catalog, Office Of The Registrar, East Tennessee State University
2009-2010 - College Of Pharmacy Catalog, Office Of The Registrar, East Tennessee State University
Pharmacy Academic Catalogs
No abstract provided.
Characterization Of The Genes Involved In Biosynthesis And Transport Of Schizokinen, A Siderophore Produced By Rhizobium Leguminosarum Iari 917., David Jack Hammond
Characterization Of The Genes Involved In Biosynthesis And Transport Of Schizokinen, A Siderophore Produced By Rhizobium Leguminosarum Iari 917., David Jack Hammond
Electronic Theses and Dissertations
Iron is the 4th most abundant metal on the earth's crust and is required by most organisms as a cofactor for many enzymes; however, at physiological pH and aerobic conditions iron forms insoluble ferric oxyhydroxide polymers. Siderophores are low molecular weight compounds that scavenge ferric ions, bind with high affinity, and transport it into the cell via multicomponent transport systems. Rhizobia are soil dwelling organisms that form symbiotic relationships with host plants and fix atmospheric nitrogen, while the bacteria receive nutrients. R. leguminosarum IARI 917 produces a siderophore characterized as 'schizokinen'. In the present study, we have characterized the binding …
Drug-Associated Changes In Amino Acid Residues In Gag P2, P7Nc, And P6Gag/P6Pol In Human Immunodeficiency Virus Type 1 (Hiv-1) Display A Dominant Effect On Replicative Fitness And Drug Response, Sarah Ho, Roxana M. Coman, Joshua C. Bunger, Stephanie L. Rose, Patricia O'Brien, Isabel Munoz, Ben M. Dunn, John W. Sleasman, Maureen M. Goodenow
Drug-Associated Changes In Amino Acid Residues In Gag P2, P7Nc, And P6Gag/P6Pol In Human Immunodeficiency Virus Type 1 (Hiv-1) Display A Dominant Effect On Replicative Fitness And Drug Response, Sarah Ho, Roxana M. Coman, Joshua C. Bunger, Stephanie L. Rose, Patricia O'Brien, Isabel Munoz, Ben M. Dunn, John W. Sleasman, Maureen M. Goodenow
ETSU Faculty Works
Regions of HIV-1 gag between p2 and p6Gag/p6Pol, in addition to protease (PR), develop genetic diversity in HIV-1 infected individuals who fail to suppress virus replication by combination protease inhibitor (PI) therapy. To elucidate functional consequences for viral replication and PI susceptibility by changes in Gag that evolve in vivo during PI therapy, a panel of recombinant viruses was constructed. Residues in Gag p2/p7NC cleavage site and p7NC, combined with residues in the flap of PR, defined novel fitness determinants that restored replicative capacity to the posttherapy virus. Multiple determinants in Gag have a dominant effect on PR phenotype and …
Community Involvement Among Liberians In Johnson City, Tennessee: An Exploratory Pilot Study., Zon Gangbayee Quewea
Community Involvement Among Liberians In Johnson City, Tennessee: An Exploratory Pilot Study., Zon Gangbayee Quewea
Electronic Theses and Dissertations
This study examined predictors of community involvement among Liberians in Johnson City, Tennessee. This study was exploratory in nature and used a social survey employing closed-ended questions. Using cross-tabulation analysis, results derived from a random sample (n = 62) of respondents indicate that persons who were older, married with children, employed, more religious, members of the Mande Fu ethnic group, and/or tended toward very liberal or conservative views had the highest rates of community participation. Predictors of types of community participation were also analyzed, the most significant of which was the higher prevalence of males in leadership roles and females …
Antigen Trafficking Within Chlamydia Trachomatis-Infected Polarized Human Endometrial Epithelial Cells., David Kelley Giles
Antigen Trafficking Within Chlamydia Trachomatis-Infected Polarized Human Endometrial Epithelial Cells., David Kelley Giles
Electronic Theses and Dissertations
Chlamydia trachomatis serovars D-K are the leading cause of bacterially-acquired sexually transmitted infections in the United States. As an obligate intracellular pathogen, C. trachomatis infects columnar epithelial cells of the genital mucosae and can cause deleterious sequelae such as pelvic inflammatory disease, infertility, and ectopic pregnancy. Several chlamydial antigens reach the host cell cytosol prior to the natural release of chlamydiae at the end of the developmental cycle. While some of these extra-inclusion antigens traffic to the host cell surface, others remain intracellular where they are proposed to influence vital host cell functions and antigen trafficking and presentation. The research …
Etsu Today - Spring 2008, University Marketing And Communication, East Tennessee State University
Etsu Today - Spring 2008, University Marketing And Communication, East Tennessee State University
ETSU Today [magazine]
Providing an excellent education for today’s college student entails many aspects of life that generations of students before them only have dreamed. Beginning with the most basic requirement of high-quality instruction in the classroom, we could build a complete system around the student that includes access to the Internet for World Wide Web use, the full services of our university libraries, online “classrooms” for entire classes, and those places students can go after the traditional schedule of class time to continue their discussions, complete assignments, and more. Learning is engaging 24 hours a day and certainly accessible well beyond a …
Activation Of Cytosolic Phospholipase A2Α In Resident Peritoneal Macrophages By Listeria Monocytogenes Involves Listeriolysin O And Tlr2, Shahid Noor, Howard Goldfine, Dawn E. Tucker, Saritha Suram, Laurel L. Lenz, Shizuo Akira, Satoshi Uematsu, Milena Girotti, Joseph V. Bonventre, Kevin Breuel, David L. Williams, Christina C. Leslie
Activation Of Cytosolic Phospholipase A2Α In Resident Peritoneal Macrophages By Listeria Monocytogenes Involves Listeriolysin O And Tlr2, Shahid Noor, Howard Goldfine, Dawn E. Tucker, Saritha Suram, Laurel L. Lenz, Shizuo Akira, Satoshi Uematsu, Milena Girotti, Joseph V. Bonventre, Kevin Breuel, David L. Williams, Christina C. Leslie
ETSU Faculty Works
Eicosanoid production by macrophages is an early response to microbial infection that promotes acute inflammation. The intracellular pathogen Listeria monocytogenes stimulates arachidonic acid release and eicosanoid production from resident mouse peritoneal macrophages through activation of group IVA cytosolic phospholipase A2 (cPLA2α). The ability of wild type L. monocytogenes (WTLM) to stimulate arachidonic acid release is partially dependent on the virulence factor listeriolysin O; however, WTLM and L. monocytogenes lacking listeriolysin O (ΔhlyLM) induce similar levels of cyclooxygenase 2. Arachidonic acid release requires activation of MAPKs by WTLM and ΔhlyLM. The attenuated release of arachidonic acid that is observed in TLR2-/- …
Office Of The Vice Provost For Research Annual Report - 2008, Office Of The Vice Provost For Research, East Tennessee State University
Office Of The Vice Provost For Research Annual Report - 2008, Office Of The Vice Provost For Research, East Tennessee State University
Annual Reports
No abstract provided.
2008-2009 - College Of Pharmacy Catalog, Office Of The Registrar, East Tennessee State University
2008-2009 - College Of Pharmacy Catalog, Office Of The Registrar, East Tennessee State University
Pharmacy Academic Catalogs
No abstract provided.
Periplasmic Phosphorylation Of Lipid A Is Linked To The Synthesis Of Undecaprenyl Phosphate, Thierry Touzé, An X. Tran, Jessica V. Hankins, Dominique Mengin-Lecreulx, M. Stephen Trent
Periplasmic Phosphorylation Of Lipid A Is Linked To The Synthesis Of Undecaprenyl Phosphate, Thierry Touzé, An X. Tran, Jessica V. Hankins, Dominique Mengin-Lecreulx, M. Stephen Trent
ETSU Faculty Works
One-third of the lipid A found in the Escherichia coli outer membrane contains an unsubstituted diphosphate unit at position 1 (lipid A 1-diphosphate). We now report an inner membrane enzyme, LpxT (YeiU), which specifically transfers a phosphate group to lipid A, forming the 1-diphosphate species. 32P-labelled lipid A obtained from lpxT mutants do not produce lipid A 1-diphosphate. In vitro assays with Kdo2-[4′- 32P]lipid A as the acceptor shows that LpxT uses undecaprenyl pyrophosphate as the substrate donor. Inhibition of lipid A 1-diphosphate formation in wild-type bacteria was demonstrated by sequestering undecaprenyl pyrophosphate with the cyclic polypeptide antibiotic bacitracin, providing …
T-Cell Dysfunction By Hcv Core Protein Involves Pd-1/Pd-L1 Signaling., Billy Ellis King
T-Cell Dysfunction By Hcv Core Protein Involves Pd-1/Pd-L1 Signaling., Billy Ellis King
Electronic Theses and Dissertations
In 1989 the hepatitis C virus was identified as a significant cause of post-transfusion hepatitis. Nearly two decades later there is still no vaccine, inadequate treatment options, and limited understanding of how the virus establishes chronicity in the majority of the people it infects. Recent reports suggest that the interaction of a negative co-stimulatory pathway mediated by PD-1 and PDL-1 is associated with persistent viral infection. The role, if any, that PD-1/PDL-1 has in HCV infection is unknown. In this study we report that PD-1 is upregulated in T-cells from persons with chronic HCV infection when compared to healthy donors. …
Examination Of Microsporidia Spore Adherence And Host Cell Infection In Vitro., Timothy Robert Southern
Examination Of Microsporidia Spore Adherence And Host Cell Infection In Vitro., Timothy Robert Southern
Electronic Theses and Dissertations
Microsporidia are obligate intracellular pathogens that cause severe disease in immunocompromised humans. While albendazole is the treatment of choice, no therapy exists that effectively treats all forms or causes of human microsporidiosis. Recent studies show that the microsporidian Encephalitozoon intestinalis binds glycosaminoglycans (GAGs) associated with the host cell surface, and that the divalent cations manganese (Mn2+) and magnesium (Mg2+) augment spore adherence to host cells by activating a constituent on the spore surface. These studies also illustrate a direct relationship between spore adherence and host cell infection; inhibition of spore adherence leads to reduced host cell …
Studies On A 50s Ribosomal Precursor Particle As A Substrate For Erm E Methyltransferase Enzyme In Staphylococcus Aureus ., Indira Pokkunuri
Studies On A 50s Ribosomal Precursor Particle As A Substrate For Erm E Methyltransferase Enzyme In Staphylococcus Aureus ., Indira Pokkunuri
Electronic Theses and Dissertations
Erythromycin is a macrolide antibiotic that inhibits not only mRNA translation but also 50S ribosomal subunit assembly in bacterial cells. An important mechanism of erythromycin resistance is the methylation of 23S rRNA by erm methyl transferase enzymes. We are interested in investigating the true substrate for methylation because it is known from our work and the work of others that fully assembled 50S subunits are not substrates for methylation. We have published a model for 50S ribosomal subunit formation where, the precursor particle that accumulates in erythromycin treated cells is a target for methyl transferase activity. Current studies are aimed …
Office Of The Vice Provost For Research Annual Report - 2007, Office Of The Vice Provost For Research, East Tennessee State University
Office Of The Vice Provost For Research Annual Report - 2007, Office Of The Vice Provost For Research, East Tennessee State University
Annual Reports
No abstract provided.
Dectin-1 Expression Is Altered By Fungal Infection, Polymicrobial Sepsis, And Glucan Administration., Tammy Regena Ozment-Skelton
Dectin-1 Expression Is Altered By Fungal Infection, Polymicrobial Sepsis, And Glucan Administration., Tammy Regena Ozment-Skelton
Electronic Theses and Dissertations
Glucans are fungal cell wall PAMPs that promote survival in polymicrobial and candidal sepsis. Dectin-1 is the primary PRR for glucans. The goals of the present study were to characterize 1) the effects of fungal infection on Dectin-1; 2) the effects of polymicrobial sepsis in the presence and absence of glucan on Dectin-1; 3) the effects of systemic administration of glucans on Dectin-1; and 4) the intracellular trafficking of glucans. Mice were either systemically infected with Candida albicans, or made septic by CLP with and without glucan phosphate (GP) injection, or injected with GP. Flow cytometry was performed to …
Latex Of Sciadopitys Verticillata (Thunb.) Siebold And Zuccarini: Antibiotic Properties, Phytochemistry, And Inhibition Of Adventitious Rooting Of Stem Cuttings., David Ira Yates
Electronic Theses and Dissertations
Sciadopitys verticillata was subjected to three propagation treatments designed to inhibit coagulation of its latex-like sap at the cut ends of the stem cuttings. Twenty-four hour soaking in water prior to rooting hormone application significantly enhanced production of adventitious roots. Old wood stem cuttings from shade-grown trees rooted at higher proportions than stem cuttings collected from sun-grown trees. Height, age, and place of origin of the source trees were not important factors in successful rooting. Antibacterial activity against some human pathogens and soil bacterial species was detected in latex application trials but the antibiotic activity was not related to the …
Intestinal Strongyloidiasis And Hyperinfection Syndrome, Raja Vadlamudi, David S. Chi, Guha Krishnaswamy
Intestinal Strongyloidiasis And Hyperinfection Syndrome, Raja Vadlamudi, David S. Chi, Guha Krishnaswamy
ETSU Faculty Works
In spite of recent advances with experiments on animal models, strongyloidiasis, an infection caused by the nematode parasite Strongyloides stercoralis, has still been an elusive disease. Though endemic in some developing countries, strongyloidiasis still poses a threat to the developed world. Due to the peculiar but characteristic features of autoinfection, hyperinfection syndrome involving only pulmonary and gastrointestinal systems, and disseminated infection with involvement of other organs, strongyloidiasis needs special attention by the physician, especially one serving patients in areas endemic for strongyloidiasis. Strongyloidiasis can occur without any symptoms, or as a potentially fatal hyperinfection or disseminated infection. Th2 cell-mediated immunity, …
Conjugative Transfer Pathways Of High-Level Mupirocin Resistance And Conjugative Transfer Genes In Staphylococcus., Danielle Barnard
Conjugative Transfer Pathways Of High-Level Mupirocin Resistance And Conjugative Transfer Genes In Staphylococcus., Danielle Barnard
Electronic Theses and Dissertations
To combat widespread infections caused by Staphylococcus aureus, mupirocin was introduced at the Veterans Affairs Medical Center, Mountain Home, Tennessee. Soon after introduction, high-level mupirocin-resistance emerged. The rapid emergence was hypothesized to be due to conjugative transfer of the mupA resistance gene from S. epidermidis to S. aureus. Results have shown that transfer of high-level mupirocin-resistance from S. aureus donors commonly occurs. However, transfer from naturally-occurring S. epidermidis donors was not attainable. Staphylococcus epidermidis transconjugants, however, were capable of serving as donors. Further examination of non-transmissibility included PCR analysis of conjugative transfer genes (tra genes) in capable …
The Cost Of Mupirocin Resistance In Staphylococcus., Susan D. Reynolds
The Cost Of Mupirocin Resistance In Staphylococcus., Susan D. Reynolds
Electronic Theses and Dissertations
Control of antibiotic resistance in bacteria is based on the concept that resistance incurs a fitness cost in non-selective conditions. Fitness costs were assessed for low- and high-level mupirocin resistance in locally-derived Staphylococcus aureus and S. epidermidis. Costs of resistance were assessed in pure cultures by comparing growth curve characteristics and in mixed culture as the proportion of resistant cells surviving. Costs were not present in comparisons of growth rates among groups of naturally-occurring isolates from the different resistance categories. However, in S. aureus, growth rates within resistance categories differed by approximately 30 – 90%. Among near-isogenic pairs …
Office Of The Vice Provost For Research Annual Report - 2006, Office Of The Vice Provost For Research, East Tennessee State University
Office Of The Vice Provost For Research Annual Report - 2006, Office Of The Vice Provost For Research, East Tennessee State University
Annual Reports
No abstract provided.
Primary Care Nurse Attitudes, Beliefs And Confidence Levels Regarding Alcohol Abuse And Its Treatment: Impact Of Educational Intervention., Raja Sekhar Vadlamudi
Primary Care Nurse Attitudes, Beliefs And Confidence Levels Regarding Alcohol Abuse And Its Treatment: Impact Of Educational Intervention., Raja Sekhar Vadlamudi
Electronic Theses and Dissertations
Alcohol abuse has been a major burden on the society. In the fight against it a key issue the education of the primary care nurses has been ignored. This study evaluates the effect of education program on the attitudes, beliefs, and confidence levels of primary care nurses regarding alcohol abuse and its treatment. Data from the Project Mainstream educational intervention were used with permission from the investigators. Two hundred one students and faculty of nursing at Vanderbilt University participated in the intervention, which was designed to train primary care providers in the Brief Negotiated Intervention technique for early detection and …
The Beta-Glucan Receptor Dectin-1 Recognizes Specific Morphologies Of Aspergillus Fumigatus, Chad Steele, Rekha R. Rapaka, Allison Metz, Shannon M. Pop, David L. Williams, Siamon Gordon, Jay K. Kolls, Gordon D. Brown
The Beta-Glucan Receptor Dectin-1 Recognizes Specific Morphologies Of Aspergillus Fumigatus, Chad Steele, Rekha R. Rapaka, Allison Metz, Shannon M. Pop, David L. Williams, Siamon Gordon, Jay K. Kolls, Gordon D. Brown
ETSU Faculty Works
Alveolar macrophages represent a first-line innate host defense mechanism for clearing inhaled Aspergillus fumigatus from the lungs, yet contradictory data exist as to which alveolar macrophage recognition receptor is critical for innate immunity to A. fumigatus. Acknowledging that the A. fumigatus cell wall contains a high beta-1,3-glucan content, we questioned whether the beta-glucan receptor dectin-1 played a role in this recognition process. Monoclonal antibody, soluble receptor, and competitive carbohydrate blockage indicated that the alveolar macrophage inflammatory response, specifically the production of tumor necrosis factor-α (TNF-α), interleukin-1α (IL-1α), IL-1β, IL-6, CXCL2/macrophage inflammatory protein-2 (MIP-2), CCL3/macrophage inflammatory protein-1α (MIP-1α), granulocyte-colony stimulating factor …
Melatonin, Immune Function And Aging, V. Srinivasan, Georges J.M. Maestroni, Daniel P. Cardinali, A. I. Esquifino, S. R. Pandi Perumal, Sandra C. Miller
Melatonin, Immune Function And Aging, V. Srinivasan, Georges J.M. Maestroni, Daniel P. Cardinali, A. I. Esquifino, S. R. Pandi Perumal, Sandra C. Miller
ETSU Faculty Works
Aging is associated with a decline in immune function (immunosenescence), a situation known to correlate with increased incidence of cancer, infectious and degenerative diseases. Innate, cellular and humoral immunity all exhibit increased deterioration with age. A decrease in functional competence of individual natural killer (NK) cells is found with advancing age. Macrophages and granulocytes show functional decline in aging as evidenced by their diminished phagocytic activity and impairment of superoxide generation. There is also marked shift in cytokine profile as age advances, e.g., CD3+ and CD4+ cells decline in number whereas CD8+ cells increase in elderly individuals. A decline in …
Etsu Today - Fall 2005, University Marketing And Communication, East Tennessee State University
Etsu Today - Fall 2005, University Marketing And Communication, East Tennessee State University
ETSU Today [magazine]
That a time of change, excitement and tremendous energy at ETSU! In fact, I consider this to be one of the most rewarding years of my professional life. It was just a year ago when I introduced our intention to create a College of Pharmacy for ETSU. Now we are within a year of seeing our first class of 65 pharmacy students begin their studies. The campus and community response has been unprecedented. With gifts ranging from anonymous $20 bills to six-figure corporate commitments, we now have in hand well over $6 million for the new college. From the inspirational …
Etsu Today - Fall 2004, University Marketing And Communication, East Tennessee State University
Etsu Today - Fall 2004, University Marketing And Communication, East Tennessee State University
ETSU Today [magazine]
The legislative session that concluded in the spring of 2004 brought much-needed hope and some tangible results to higher education in Tennessee. Realizing that work still must be done, we are greatly appreciative of the salary improvements that were approved for our faculty and staff. ETSU also succeeded in acquiring some $11 million in capital outlay funding to renovate the old Sherrod Library, providing critically needed space for our College of Nursing. We have the largest nursing enrollment in Tennessee, and we have plans to increase enrollment in the B.S.N. program by 50 percent, a major step in our continued …
2003-2004 - Graduate Council Annual Report, College Of Graduate And Continuing Studies, East Tennessee State University
2003-2004 - Graduate Council Annual Report, College Of Graduate And Continuing Studies, East Tennessee State University
Graduate Council Agendas and Minutes
No abstract provided.