Digital Commons Network^™

Advances In Cystic Fibrosis, Courtney Utley, Kristen L. Mchenry

ETSU Faculty Works

The purpose of this review was to identify the history of and advances in cystic fibrosis (CF). New treatment plans, medication developments, and a historical perspective of airway clearance therapy (ACT) will be presented. The importance of treatment compliance and time management in the care of cystic fibrosis patients will also be discussed. Furthermore, the development of cystic fibrosis clinics and the pivotal role they play in the treatment of the disease will be addressed. Lastly, a brief discussion concerning the need for and process of lung transplantation will be reported.

The Eco-Smart Can, Darack B. Nanto

Undergraduate Honors Theses

I noticed that maintenance workers had the same itinerary when emptying trashcans, meanwhile some trashcans needed to be emptied urgently. Traditionally, ETSU maintenance operate on daily routes to pick trash on designated time, regardless the level of the containers. The time, resources and labor invested in collecting the trash could be saved. Therefore, I decided to use the Internet of Things (IoT) to create a device that will optimize trash collection, to reduce costs and pollution.

2016-November-10-Beaumont Research, University Marketing And Communications, East Tennessee State University

ETSU News

No abstract provided.

2016-October-27-Rdc Study, University Marketing And Communications, East Tennessee State University

ETSU News

No abstract provided.

Illuminated Magazine, School Of Graduate Studies, East Tennessee State University

Illuminated: Graduate Student Research Magazine

From the School of Graduate Studies; Research Grant Award Recipients; Cesium Adsorption in the Environment [Kenneth Seaton Chemistry, M.S.]; Social Media and Health Information [Anthony Peluso Public Health, M.P.H]; Where Are They Now? [Samuel Kakraba Mathematical Sciences, M.S., 2015 Colton Watts Biology, M.S., 2014]; The Forgotten Law Enforcement Officers [Caitlin Botelho, Sociology, M.A.]; Asking the Right Questions [Jedaidah Chilufya Biology, M.S]; Where Are They Now? [Saundra Reynolds Liberal Studies, M.A.L.S., 2016 Kathleen Klik Psychology, Ph.D., 2015]; The Epidemiology of Hepatitis C [Yang Chen Public Health, Dr.P.H]; Criminal Justice and Sex Offenders [Maria Aparcero-Seuro Criminal Justice and Criminology, M.A.]; Student Affairs: …

2016-August-19-2016 Distinguished Faculty Awards, University Marketing And Communications, East Tennessee State University

ETSU News

No abstract provided.

Using Scientific Inquiry To Increase Knowledge Of Vaccine Theory And Infectious Diseases, Zachary Walls, John B. Bossaer, David Cluck

ETSU Faculty Works

Background: The aim of this study was to design and evaluate a laboratory activity based on scientific inquiry to educate first-year pharmacy students in the U.S. about vaccination theory and the attributes of common pathogens. Methods: The laboratory activity had two principal sections. The first consisted of an interactive game during which students rolled a die to determine outcomes based on a set of pre-determined criteria. In the second section, students generated and tested hypotheses about vaccine theory using a computer simulation that modeled disease transmission within a large population. In each section students were asked to evaluate epidemiological data …

Inactivation Of Type I Ifn Jak-Stat Pathway In Ebv Latency, Shunbin Ning, Ling Wang

ETSU Faculty Works

Epstein-Barr Virus (EBV) latent infection is associated with a variety of lymphomas and carcinomas. Interferon (IFN) Regulatory Factors (IRFs) are a family of transcription factors, among which IRF7 is the “master” regulator of type I IFNs (IFN-I) that defends against invading viruses. Robust IFN-I responses require a positive feedback loop between IRF7 and IFN-I. In recent years, we have discovered that IRF7 is significantly induced and activated by the principal EBV oncoprotein--Latent Membrane Protein 1 (LMP1); however, IRF7 fails to trigger robust IFN-I responses in EBV latency. We believe this intriguing finding is critical for EBV latency and oncogenesis, yet …

Erratum: Erratum To “A 78 Years Old Male With Painful Erythematous Skin Lesions In Lower Extremity”: [International Journal Of Infectious Diseases, 2016 48, Pages 32] International Journal Of Infectious Diseases 2016 49 170, Gilbert Bader, Mohammed Al-Tarawneh, Mohsen Pourmorteza, Wael Shams

ETSU Faculty Works

The publisher regrets that the paper was published without Figures 1, 2 and 3. The publisher would like to apologise for any inconvenience caused.

Host Nectin-1 Promotes Chlamydial Infection In The Female Mouse Genital Tract, But Is Not Required For Infection In A Novel Male Murine Rectal Infection Model, Jessica A. Slade, Jennifer V. Hall, Jennifer Kintner, Regenia Phillips-Campbell, Robert V. Schoborg

ETSU Faculty Works

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Chlamydia trachomatis is the most common bacterial sexually transmitted pathogen, but more than 70% of patients fail to seek treatment due to the asymptomatic nature of these infections. Women suffer from numerous complications from chronic chlamydial infections, which include pelvic inflammatory disease and infertility. We previously demonstrated in culture that host cell nectin-1 knockdown significantly reduced chlamydial titers and inclusion size. Here, we sought to determine …

Endothelial Hspa12b Is A Novel Protein For The Preservation Of Cardiovascular Function In Polymicrobial Sepsis Via Exosome Mir-126, Xia Zhang

Electronic Theses and Dissertations

Sepsis is the most frequent cause of mortality in most intensive care units. Cardiovascular dysfunction is a major complication associated with sepsis, with high mortality rates up to 70%. Currently, there is no effective treatment approach for sepsis.

The integrity of the endothelium is fundamental for the homeostasis of the cardiovascular system. Sepsis induces endothelial cell injury which is the key factor for multiple organ failure. The increased expression of adhesion molecules and chemokines in endothelial cell promotes leukocytes infiltration into the tissue. The loss of tight junction proteins and increased permeability of the endothelial cells will provoke tissue hypoxia …

Extraction, Purification And Characterization Of An Antibiotic-Like Compound Produced By Rhodococcus Sp. Mtm3w5.2, Pushpavathi Reddyvari Manikindi

Electronic Theses and Dissertations

The bacterium Rhodococcus is a potential source for novel antimicrobial metabolites. Recently, the Rhodococcus strain MTM3W5.2 was isolated from a soil sample collected from Morristown, East Tennessee and was found to produce an inhibitor molecule that is active against similar Rhodococcus species. The aim of this research is to extract, purify, and characterize the active compound. The compound was obtained from both agar and broth cultures of strain MTM3W5.2 and purified by primary fractionation of crude extract on a Sephadex LH-20 column, followed by semi-preparative reversed phase column chromatography. Final purification was achieved using multiple rounds of an analytical C …

A 78-Year-Old Male With Painful Erythematous Skin Lesions On The Lower Extremity, Gilbert Bader, Mohammed Al-Tarawneh, Mohsen Pourmorteza, Wael Shams, Eskild Petersen

ETSU Faculty Works

No abstract provided.

2015-2016 Department Of Internal Medicine Scholarly Activity Report, Quillen College Of Medicine, East Tennessee State University

Quillen College of Medicine Scholarly Activity Reports

No abstract provided.

2015-2016 Department Of Biomedical Sciences Scholarly Activity Report, Quillen College Of Medicine, East Tennessee State University

Quillen College of Medicine Scholarly Activity Reports

No abstract provided.

2016-June-24-Ciidi Update, University Marketing And Communications, East Tennessee State University

ETSU News

No abstract provided.

Expansion Of Myeloid-Derived Suppressor Cells Promotes Differentiation Of Regulatory T Cells In Hiv-1+ Individuals, Ling Wang, Juan Zhao, Junping P. Ren, Xiao Y. Wu, Zheng D. Morrison, Mohamed A. El Gazzar, Shunbin Ning, Jonathan P. Moorman, Zhi Q. Yao

ETSU Faculty Works

Objective: Regulatory T cells (Tregs) contribute to HIV-1 disease progression by impairing antiviral immunity; however, the precise mechanisms responsible for the development of Tregs in the setting of HIV-1 infection are incompletely understood.

Design: In this study, we provide evidence that HIV-induced expansion of monocytic myeloid-derived suppressor cells (M-MDSCs) promote the differentiation of Foxp3+ Tregs.

Methods: We measured MDSC induction and cytokine expression by flow cytometry and analyzed their functions by coculturing experiments.

Results: We observed a dramatic increase in M-MDSC frequencies in the peripheral blood of HIV-1 seropositive (HIV-1+) individuals, even in those on antiretroviral therapy with undetectable viremia, …

2016-May-26-Trained Immunity, University Marketing And Communications, East Tennessee State University

ETSU News

No abstract provided.

Protein Phosphatase 1 Abrogates Irf7-Mediated Type I Ifn Response In Antiviral Immunity, Ling Wang, Juan Zhao, Junping Ren, Kenton H. Hall, Jonathan P. Moorman, Zhi Q. Yao, Shunbin Ning

ETSU Faculty Works

Interferon (IFN) regulatory factor 7 (IRF7) plays a key role in the production of IFN‐α in response to viral infection, and phosphorylation at IRF7 C‐terminal serine sites is prelude to its function. However, phosphatases that negatively regulate IRF7 phosphorylation and activity have not been reported. In this study, we have identified a conserved protein phosphatase 1 (PP1)‐binding motif in human and mouse IRF7 proteins, and shown that PP1 physically interacts with IRF7. Exogenous expression of PP1 subunits (PP1α, β, or γ) ablates IKKε‐stimulated IRF7 phosphorylation and dramatically attenuates IRF7 transcriptional activity. Inhibition of PP1 activity significantly increases IRF7 phosphorylation and …

The Rise And Fall Of Efavirenz, David Cluck, Paul Lewis, Spencer H. Durham, E. Kelly Hester

ETSU Faculty Works

No abstract provided.

Ecological Niche Modeling Of The North American Giant Salamander: Predicting Current And Future Potential Distributions And Examining Environmental Influences, Selena S. Roark

Electronic Theses and Dissertations

North American Giant Salamanders (Cryptobranchus alleganiensis), commonly known as hellbenders, have been experiencing a population decline for decades due to human influences, such as pollution and habitat destruction. Many efforts are underway to save the hellbender but their entire potential geographical range has not been well-studied. Currently, hellbender populations are delineated by county boundaries and are on the International Union for Conservation of Nature Red List. The Genetic Algorithm for Rule-set Production, an Ecological Niche Model, was used to model the current hellbender potential distribution at a macro-scale under two different environmental scenarios. Additionally, future potential distributions were …

Comparison Of Anti-Pneumococcal Functions Of Native And Modified Forms Of C-Reactive Protein, Donald Neba Ngwa

Electronic Theses and Dissertations

The anti-pneumococcal function of native C-reactive protein (CRP) involves its binding to phosphocholine molecules present on Streptococcus pneumoniae and subsequent activation of the complement system. However, when pneumococci recruit complement inhibitory protein factor H on their surface, they escape complement attack. Non-native forms of CRP have been shown to bind immobilized factor H. Accordingly, we hypothesized that modified CRP would bind to factor H on pneumococci, masking its complement inhibitory activity, allowing native CRP to exert its anti-pneumococcal function. As reported previously, native CRP protected mice from lethal pneumococcal infection when injected 30 minutes before infection but not when injected …

Alignment Of Epidemiology Practice And Academic Competencies Through Effective Collaboration, Kimberly R. Glenn, Paula R. Masters, Megan Quinn

ETSU Faculty Works

Background: Online learning has recently garnered increased attention as technology use in the classroom grows. However, most of the published approaches regarding this topic in postgraduate education centers on clinical environments. Models of partnerships between applied public health agencies and academic centers to produce mutually beneficial online learning opportunities for graduate-level public health courses have not been explored in the literature.

Methods: East Tennessee State University (ETSU) and the Tennessee Department of Health (TDH) partnered to build three online, asynchronous epidemiology modules for an interdisciplinary audience of graduate students. The goals of the modules were to (1) introduce students to …

2016-March-24-Inflammation And Aging, University Marketing And Communications, East Tennessee State University

ETSU News

No abstract provided.

Hcv-Induced Mir146a Controls Socs1/Stat3 And Cytokine Expression In Monocytes To Promote Regulatory T-Cell Development, Junping Ren, Rue S. Ying, Yong Q. Cheng, Ling Wang, Mohamed A. El Gazzar, Guang Y. Li, Shun B. Ning, Jonathon P. Moorman, Zhi Q. Yao

ETSU Faculty Works

Host innate and adaptive immune responses must be tightly regulated by an intricate balance between positive and negative signals to ensure their appropriate onset and termination while fighting pathogens and avoiding autoimmunity; persistent pathogens may usurp these regulatory machineries to dampen host immune responses for their persistence in vivo. Here, we demonstrate that miR146a is up‐regulated in monocytes from hepatitis C virus (HCV )‐infected individuals compared to control subjects. Interestingly, miR146a expression in monocytes without HCV infection increased, whereas its level in monocytes with HCV infection decreased, following Toll‐like receptor (TLR ) stimulation. This miR146a induction by HCV infection …

Chlamydial Antibiotic Resistance And Treatment Failure In Veterinary And Human Medicine, Nicole Borel, Cory Leonard, Jessica Slade, Robert V. Schoborg

ETSU Faculty Works

The Chlamydiaceae are widespread pathogens of both humans and animals. Chlamydia trachomatis infection causes blinding trachoma and reproductive complications in humans. Chlamydia pneumoniae causes human respiratory tract infections and atypical pneumonia. Chlamydia suis infection is associated with conjunctivitis, diarrhea, and failure to gain weight in domestic swine. Chlamydial infections in humans and domesticated animals are generally controlled by antibiotic treatment—particularly macrolides (usually azithromycin) and tetracyclines (tetracycline and doxycycline). Tetracycline-containing feed has also been used to limit infections and promote growth in livestock populations, although its use has decreased because of growing concerns about antimicrobial resistance development. Because Sandoz and Rockey …

2016-February-29-Research Recognized, University Marketing And Communications, Est Tennessee State University

ETSU News

No abstract provided.

2016-February-05, University Marketing And Communications, East Tennessee State University

ETSU News

No abstract provided.

Tslp Production By Dendritic Cells Is Modulated By Il-1Β And Components Of The Endoplasmic Reticulum Stress Response, Matthew J. Elder, Steven J. Webster, David L. Williams, J. S.Hill Gaston, Jane C. Goodall

ETSU Faculty Works

Thymic stromal lymphopoietin (TSLP) produced by epithelial cells acts on dendritic cells (DCs) to drive differentiation of TH2-cells, and is therefore important in allergic disease pathogenesis. However, DCs themselves make significant amounts of TSLP in response to microbial products, but little is known about the key downstream signals that induce and modulate this TSLP secretion from human DCs. We show that human monocyte derived DC (mDC) secretion of TSLP in response to Candida albicans and β-glucans requires dectin-1, Syk, NF-κB, and p38 MAPK signaling. In addition, TSLP production by mDCs is greatly enhanced by IL-1β, but not TNF-α, in contrast …

2016-January-26-Etsu Scientist Researching Impact Of Hormones On Chlamydial Infections, University Marketing And Communications, East Tennessee State University

ETSU News

No abstract provided.