Digital Commons Network^™

2016-January-25-Antimicrobial Stewardship, University Marketing And Communications, East Tennessee State University

ETSU News

No abstract provided.

2016-01-15-Jennifer Hall, University Marketing And Communications, East Tennessee State University

Accent [newsletter]

No abstract provided.

Chlamydial Pre-Infection Protects From Subsequent Herpes Simplex Virus-2 Challenge In A Murine Vaginal Super-Infection Model, Jessica Slade, Jennifer V. Hall, Jennifer Kintner, Robert V. Schoborg

ETSU Faculty Works

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Chlamydia trachomatis and Herpes Simplex Virus-2 (HSV-2) genital tract co-infections have been reported in humans and studied in vitro but the clinical consequences are unknown. Limited epidemiologic evidence suggests that these co-infections could be more severe than single infections of either pathogen, but the host-pathogen interactions during co-infection remain uncharacterized. To determine whether disease progression and/or pathogen shedding differs between singly-infected and super-infected animals, we developed …

2016-2017 - College Of Pharmacy Catalog, Office Of The Registrar, East Tennessee State University

Pharmacy Academic Catalogs

No abstract provided.

Office Of The Vice Provost For Research Annual Report - 2016, Office Of The Vice Provost For Research, East Tennessee State University

Annual Reports

No abstract provided.

Diminished Quality Of Life Among Women Affected By Ebola, Jessi Hanson, Alexis Decosimo, Megan Quinn

ETSU Faculty Works

This article analyzes data collected from Liberian women afflicted by the Ebola virus disease, survivors of the virus and noninfected persons living in Ebola-affected homes. This research is one of the first statistical analyses examining factors diminishing quality of life: negative experiences, stigma, and psychosocial symptoms among females affected by the virus after the outbreak. The research presents a thorough literature review, including research related to other infectious diseases like HIV/AIDS, to inform the gap in studies on Ebola’s effects on quality of life. Women who are Ebola virus disease survivors demonstrate significant differences in stigma and psychosocial stress when …

Cell Wall N-Linked Mannoprotein Biosynthesis Requires Goa1p, A Putative Regulator Of Mitochondrial Complex I In Candida Aabicans, Xiaodong She, Richard Calderone, Michael Kruppa, Douglas Lowman, David Williams, Lili Zhang, Ying Gao, Kasra Khamooshi, Weida Liu, Dongmei Li

ETSU Faculty Works

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. The Goa1p of Candida albicans regulates mitochondrial Complex I (CI) activities in its role as a putative CI accessory protein. Transcriptional profiling of goa1Δ revealed a down regulation of genes encoding β-oligomannosyl transferases. Herein, we present data on cell wall phenotypes of goa1Δ (strain GOA31). We used transmission electron microscopy (TEM), GPC/MALLS, and NMR to compare GOA31 to a gene-reconstituted strain (GOA32) and parental cells. We …

Microrna-214 Protects Against Hypoxia/Reoxygenation Induced Cell Damage And Myocardial Ischemia/Reperfusion Injury Via Suppression Of Pten And Bim1 Expression, Xiaohui Wang, Tuanzhu Ha, Yuanping Hu, Chen Lu, Li Liu, Xia Zhang, Race Kao, John Kalbfleisch, David Williams, Chuanfu Li

ETSU Faculty Works

Background: Myocardial apoptosis plays an important role in myocardial ischemia/reperfusion (I/R) injury. Activation of PI3K/Akt signaling protects the myocardium from I/R injury. This study investigated the role of miR-214 in hypoxia/ reoxygenation (H/R)-induced cell damage in vitro and myocardial I/R injury in vivo. Methods and Results: H9C2 cardiomyoblasts were transfected with lentivirus expressing miR-214 (LmiR-214) or lentivirus expressing scrambled miR-control (LmiR-control) respectively, to establish cell lines of LmiR-214 and LmiR-control. The cells were subjected to hypoxia for 4 h followed by reoxygenation for 24 h. Transfection of LmiR-214 suppresses PTEN expression, significantly increases the levels of Akt phosphorylation, markedly attenuates …

Breathless At The Point Of A Sword, Pooja Sethi, Zia Ur Rahman, Terry Forest, Timir Paul

ETSU Faculty Works

Context: Scimitar syndrome is a congenital anomaly of pulmonary venous return where right pulmonary artery drains into right side other heart, instead of the left side, causing pulmonary hypertension resulting in shortness of breath, recurrent lower respiratory tract infections, chest pain, and fatigue. Early diagnosis and surgical intervention would correct this congenital anomaly reducing morbidity and complications in otherwise healthy young patients. Case Report: We present a case of an 18-year-old female who presented with exertional shortness of breath, fatigue, and recurrent lower respiratory tract infections. She had unremarkable physical examination but chest x-ray showed an abnormal opacity next to …

Demography And Disease Of The Rare Shrub Buckleya Distichophylla (Santalaceae) In Northeastern Tennessee, William Seth Ratliff

Undergraduate Honors Theses

Piratebush (Buckleya distichophylla (Nutt.) Torr.) is a rare, hemiparasitic shrub with the only extant populations in western North Carolina, northeastern Tennessee, and southwestern Virginia. The preferred natural hosts of piratebush, Carolina and eastern hemlocks, have seen sharp declines over the last decade due to the invasive hemlock woolly adelgid. Virginia pine, another important host of piratebush, is also susceptible to disease, specifically Cronartium appalachianum, a rust fungus for which piratebush is the secondary host. This study described and analyzed current demographic parameters of three Tennessee piratebush populations. Additionally, spatial patterns of disease and demographic characters were analyzed. These …

Orally Delivered Β-Glucans Aggravate Dextran Sulfate Sodium (Dss)-Induced Intestinal Inflammation, Sigrid E.M. Heinsbroek, David L. Williams, Olaf Welting, Sybren L. Meijer, Siamon Gordon, Wouter J. De Jonge

ETSU Faculty Works

β-Glucans have beneficial health effects due to their immune modulatory properties. Oral administration of β-glucans affects tumour growth, microbial infection, sepsis, and wound healing. We hypothesized that pre-treatment with orally delivered soluble and particulate β-glucans could ameliorate the development of aggravate dextran sulfate sodium (DSS) induced intestinal inflammation. To study this, mice were orally pre-treated with β-glucans for 14 days. We tested curdlan (a particulate β-(1,3)-glucan), glucan phosphate (a soluble β-(1,3)-glucan), and zymosan (a particle made from Saccharomyces cerevisiae, which contains around 55% β-glucans). Weight loss, colon weight, and feces score did not differ between β-glucan and vehicle treated groups. …

A Physiologically-Based Pharmacokinetic Model For Vancomycin, Rebekah White

Undergraduate Honors Theses

Vancomycin is an antibiotic used for the treatment of systemic infections. It is given

intravenously usually every twelve or twenty-four hours. This particular drug has a

medium level of boundedness, with approximately fty percent of the drug being free

and thus physiologically eective. A physiologically-based pharmacokinetic (PBPK)

model was used to better understand the absorption, distribution, and elimination of

the drug. Using optimal parameters, the model could be used in the future to test

how various factors, such as BMI or excretion levels, might aect the concentration

of the antibiotic.

2015-11-23-New Employees, University Marketing And Communications, East Tennessee State University

Accent [newsletter]

No abstract provided.

2015-November-13-Research Team Studying Link Between Genetics, Drug Side Effects, University Marketing And Communications, East Tennessee State University

ETSU News

No abstract provided.

2015-November-03-Etsu To Live-Stream 24 Hours Of Reality For Climate Change Roundtable, University Marketing And Communications, East Tennessee State University

ETSU News

No abstract provided.

2015-10-27-Professor Receives Nih Funds To Research Liver Disease, University Marketing And Communications, East Tennessee State University

Accent [newsletter]

No abstract provided.

2015-October-16-Professor Receives Federal Grant To Study Protein To Treat Cirrhosis Johnson City, University Marketing And Communications, East Tennessee State University

ETSU News

No abstract provided.

Illuminated Magazine, School Of Graduate Studies, East Tennessee State University

Illuminated: Graduate Student Research Magazine

From the School of Graduate Studies; Where Are They Now? [Featuring Wendee White, Ariel Ford, Dr. Gene Couch & Tasha Keys]; Planning for Success [Dr. Wendy Doucette Graduate Studies Librarian]; Oregon’s Right to Choose: Decisions About Dying with Dignity [Sociology, M.A. Graduate Student: Erin Mauck]; When Pathogens Don’t Share Well: Exploring How Chlamydial Infections Prevent Herpes Virus Disease [Biomedical Sciences, Ph.D. Graduate Student: Jessica Slade]; Improving Education Through Research [Elementary Education, M. Ed. Graduate Student: Kelly Stapleton]; Controlling Lipid Synthesis [Biomedical Sciences, Ph.D. Graduate Student: Muhammad Mahbubur Rahman]; English [English, M.A. Graduate Student: Inga Sarkodie]; The Mechanism of Atr and …

2015-08-24-Etsu Welcomes New Employees, University Marketing And Communications, East Tennessee State University

Accent [newsletter]

No abstract provided.

Microbial Ligand Costimulation Drives Neutrophilic Steroid-Refractory Asthma, Sabelo Hadebe, Frank Kirstein, Kaat Fierens, Kong Chen, Rebecca A. Drummond, Simon Vautier, Sara Sajaniemi, Graeme Murray, David L. Williams, Pierre Redelinghuys, Todd A. Reinhart, Beth A.Fallert Junecko, Jay K. Kolls, Bart N. Lambrecht, Frank Brombacher, Gordon D. Brown, Bernhard Ryffel

ETSU Faculty Works

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Asthma is a heterogeneous disease whose etiology is poorly understood but is likely to involve innate responses to inhaled microbial components that are found in allergens. The influence of these components on pulmonary inflammation has been largely studied in the context of individual agonists, despite knowledge that they can have synergistic effects when used in combination. Here we have explored the effects of LPS and β-glucan, …

Damage/Danger Associated Molecular Patterns (Damps) Modulate Chlamydia Pecorum And C. Trachomatis Serovar E Inclusion Development In Vitro, Cory Ann Leonard, Robert V. Schoborg, Nicole Borel

ETSU Faculty Works

Persistence, more recently termed the chlamydial stress response, is a viable but non-infectious state constituting a divergence from the characteristic chlamydial biphasic developmental cycle. Damage/danger associated molecular patterns (DAMPs) are normal intracellular components or metabolites that, when released from cells, signal cellular damage/ lysis. Purine metabolite DAMPs, including extracellular ATP and adenosine, inhibit chlamydial development in a species-specific manner. Viral co-infection has been shown to reversibly abrogate Chlamydia inclusion development, suggesting persistence/chlamydial stress. Because viral infection can cause host cell DAMP release, we hypothesized DAMPs may influence chlamydial development. Therefore, we examined the effect of extracellular ATP, adenosine, and cyclic …

Identification Of Genes Required To Synthesize An Antibiotic-Like Compound From The Soil Bacterium Rhodococcus Sp. Mtm3w5.2, Amber L. Ward

Electronic Theses and Dissertations

Rhodococcus is a soil bacterium, member of the Actinobacteria, and a close relative of the prolific small molecule producer Streptomyces. Recent interest in Rhodococcus as an under investigated source of possible bioactive secondary metabolites is sparked by the discovery of many polyketide synthase and non-ribosomal peptide synthetase genes of unknown function from sequenced Rhodococcus genomes. Rhodococcus species strain MTM3W5.2 was recently shown to produce a strong inhibitory compound with activity against most strains of Rhodococcus and closely related genera. A goal of this investigation is to discover the gene(s) required to synthesize this inhibitory molecule. The engineered Rhodococcus …

Minority College Students’ Attitudes And Beliefs Regarding The Profession Of Dental Hygiene In Comparison To Their Oral Health And Dental Knowledge, Trina J. Morgan

Electronic Theses and Dissertations

The purpose of this study was to find out the attitudes and beliefs of minority college students enrolled at Missouri College in Brentwood, Missouri in reference to the dental hygiene profession. In particular, does their oral health and dental knowledge relate to their knowledge of profession of dental hygiene? One hundred and six students gave their consent to participate in the study via Survey Monkey. The study was conducted in May 2015 for a period of four weeks. Four statements were designed to gauge minority students’ knowledge of dental hygiene as a career. No differences were found based on gender, …

2014-2015 - Graduate Council Annual Report, College Of Graduate And Continuing Studies, East Tennessee State University

Graduate Council Agendas and Minutes

No abstract provided.

Noroviruses As A Cause Of Diarrhea In Immunocompromised Pediatric Hematopoietic Stem Cell And Solid Organ Transplant Recipients, X. Ye, J. N. Van, F. M. Munoz, P. A. Revell, Claudia A. Korinetz, R. A. Krance, R. L. Atmar, M. K. Estes, H. L. Koo

ETSU Faculty Works

Case reports describe significant norovirus gastroenteritis morbidity in immunocompromised patients. We evaluated norovirus pathogenesis in prospectively enrolled solid organ (SOT) and hematopoietic stem cell transplant (HSCT) patients with diarrhea who presented to Texas Children's Hospital and submitted stool for enteric testing. Noroviruses were detected by real-time reverse transcription polymerase chain reaction. Clinical outcomes of norovirus diarrhea and non-norovirus diarrhea patients, matched by transplanted organ type, were compared. Norovirus infection was identified in 25 (22%) of 116 patients, more frequently than other enteropathogens. Fifty percent of norovirus patients experienced diarrhea lasting ≥14 days, with median duration of 12.5 days (range 1–324 …

2014-2015 Department Of Obstetrics And Gynecology Scholarly Activity Report, Quillen College Of Medicine, East Tennessee State University

Quillen College of Medicine Scholarly Activity Reports

No abstract provided.

2014-2015 Department Of Internal Medicine Scholarly Activity Report, Quillen College Of Medicine, East Tennessee State University

Quillen College of Medicine Scholarly Activity Reports

No abstract provided.

2014-2015 Department Of Biomedical Sciences Scholarly Activity Report, Quillen College Of Medicine, East Tennessee State University

Quillen College of Medicine Scholarly Activity Reports

No abstract provided.

Biological Sciences Newsletter - May 2015, Department Of Biological Sciences, East Tennessee State University

Biological Sciences Newsletters

No abstract provided.

Identification Of Transcription Factors Gzf3, Rfx1, Orf19.3928 As Being Implicated In Candida-Bacterial Interactions., Joni Watson

Undergraduate Honors Theses

Candida albicans is an opportunistic pathogen that is present in the normal flora in a majority of individuals. One key factor in C. albicans virulence is the ability to change its morphology from yeast to an elongated or hyphal form. The regulation of this morphogenesis relies in part upon quorum sensing (QS) molecules. C. albicans often exists as part of a mixed culture alongside other microbes and is influenced by their presence as well as the presence of QS molecules that they produce. In this study, a library of diploid homozygous transcriptional regulator knockout (TRKO) mutants were screened to identify …