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Plasma Membrane Insertion Of Kca2.3 (Sk3) Is Dependent Upon The Snare Proteins, Syntaxin-4 And Snap23, Claudia A. Bertuccio, Tony T. Wang, Kirk L. Hamilton, Diego J. Rodriguez-Gil, Steven B. Condliffe, Daniel C. Devor

ETSU Faculty Works

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. We previously demonstrated endocytosis of KCa2.3 is caveolin-1-, dynamin II- and Rab5-dependent. KCa2.3 then enters Rab35/EPI64C- and RME-1-containing recycling endosomes and is returned to the plasma membrane (PM). Herein, we report on the mechanism by which KCa2.3 is inserted into the PM during recycling and following exit from the Golgi. We demonstrate KCa2.3 colocalizes with SNAP-23 and Syntaxin-4 in the PM of HEK and endothelial cells …

Does Spaceflight Increase The Chance Of Female Astronauts Developing Uterine Cancer?, Areej Mosa

Electronic Theses and Dissertations

One of the main questions put forth by NASA and the European Space Agency (ESA) is whether or not an organism, especially mankind, can complete an entire life cycle in space. With this in mind, it is essential to study the effect of spaceflight on reproductive tissues. Using simulated microgravity techniques and whole-body radiation we sought to determine if females subjected to a simulated spaceflight environment have increased incidences of uterine cancer. Uterine tissue from mice subjected to simulated spaceflight was analyzed using immunohistochemical staining and western blot analysis. Two pathways commonly activated in cancer were investigated. Additionally, the uterine …

Hiv-Related Knowledge, Attitudes, And Behaviors In Two Low Resource Settings, Candice Lynn Collins

Electronic Theses and Dissertations

Two Human Immunodeficiency Virus (HIV) outbreaks occurred almost simultaneously in the United States (US) (2014-2015) and in Cambodia (2015). Information is lacking on HIV-related knowledge, stigmatizing attitudes, and high-risk behaviors (KAB) among the general population, which may affect the transmission of HIV and lead to outbreaks. The current study aimed to: 1) assess KAB among the general population in a high-risk county in the US, 2) analyze KAB among the general population of Cambodia, and 3) compare KAB across samples from a high-risk county in Northeast Tennessee and a province in Cambodia. Tennessee data were collected in 2017 and Cambodian …

Myths, Risks, And Ignorance: Western Media And Health Experts’ Representations Of Cultures In Ebola-Affected West African Communities, Samson Wonnah

Electronic Theses and Dissertations

The 2014 Ebola outbreak, mostly affecting Liberia, Sierra Leone, and Guinea, is the largest ever recorded. The Ebola response encountered resistance in some affected communities, where some residents accused relief agencies from the Global North of denigrating local cultures. This thesis examines mainstream Western media and health experts’ representation of culture in the Ebola-affected region and employed Foucauldian analysis of discursive power to discuss the impact of such a representation on the concerned communities. Through a content analysis of selected journal and news articles by Western scholars and media and official reports by some relief agencies involved with the Ebola …

The Inhibitory Effects Of A Novel Gel On Staphylococcus Aureus Biofilms, Lindsey Vance

Undergraduate Honors Theses

Antibiotic resistance is an ever-growing topic of concern within the medical field causing researchers to examine the mechanisms of resistance to develop new antimicrobials. Bacteria’s ability to form biofilms is one mechanism which aids in antimicrobial resistance. Staphylococcus aureus is of special interest as it is one of the most frequent biofilm-forming bacteria found on medical devices causing infections and posing dangerous threats in a clinical setting. A recently developed antimicrobial gel has been shown to have profound effects on treating bacterial infections and wound healing. This research is centered upon examining the antimicrobial effects of this gel on the …

Interdisciplinary Knowledge Of Etsu Dental Hygiene And Nutrition Students, Jade Merritt

Undergraduate Honors Theses

There is a significant relationship between nutrition, systemic disease, and oral health. The integration of both nutrition and oral health information into educational programs for dental hygiene and nutrition students is vital for each profession. The purpose of this study was to determine the knowledge levels of dental hygiene and nutrition students in regards to content material pertaining to each discipline. This study attempted to provide baseline data for those teaching in ETSU’s Dental Hygiene and Nutrition programs to determine if modifications in the curricula are needed to better prepare future students. Results indicated that there was not a significant …

2018-April-30-Liver Disease Screening Grant, University Marketing And Communications, East Tennessee State University

ETSU News

No abstract provided.

2018 April 27 - Board Of Trustees Meeting, Board Of Trustees, East Tennessee State University

Board of Trustees Agendas and Minutes

No abstract provided.

2018 April 27 - Board Of Trustees Academic And Student Affairs Committee, Board Of Trustees, East Tennessee State University

Board of Trustees Agendas and Minutes

No abstract provided.

2018-April-11-Liver Disease Screening, University Marketing And Communications, East Tennessee State University

ETSU News

No abstract provided.

Differential Diagnosis Of Pan-Uveitis: Behçet’S Disease, Peter Blosser, Remil Simon, Courtney Ridner

Appalachian Student Research Forum

This report describes the case of a 56-year-old man who presented with blurry vision, increased intraocular pressure, and conjunctival injection after posterior chamber intraocular lens implantation. Initially post-operative endophalmitis and foreign body inflammation were considered as differential diagnoses, but after further examination pan-uveitis was diagnosed. Uveitis is an ocular finding that may indicate several diseases, one of which is Behçet’s Disease. During the interview, the patient mentioned a history of apthous ulcers and genital ulcers which then lead to the clinical diagnosis of Behçet’s Disease. This report emphasizes that Behçet’s Disease is rare in Caucasians. Therefore, is frequently misdiagnosed in …

Nf2 Signaling Pathway Plays A Pro-Apoptotic Role In Β-Adrenergic Receptor Stimulated Cardiac Myocyte Apoptosis, Suman Dalal, Barbara Connelly, Mahipal Singh, Krishna Singh

ETSU Faculty Works

This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication. β-adrenergic receptor (β-AR) stimulation induces cardiac myocyte apoptosis in vitro and in vivo. Neurofibromin 2 (NF2) is a member of the ezrin/radixin/moesin (ERM) family of proteins. Post-translational modifications such as phosphorylation and sumoylation affect NF2 activity, subcellular localization and function. Here, we tested the hypothesis that β-AR stimulation induces post-translational modifications of NF2, and NF2 plays a pro-apoptotic …

2018-February-15-Dr. Ling Wang, University Marketing And Communications, East Tennessee State University

ETSU News

No abstract provided.

2018-February-05-A Better Treatment, University Marketing And Communications, East Tennessee State University

ETSU News

No abstract provided.

2018-February-05-A Better Treatment, University Marketing And Communications, East Tennessee State University

ETSU News

No abstract provided.

Lactate And Immunosuppression In Sepsis, Benjamin Nolt, Fei Tu, Xiaohui Wang, Tuanzhu Ha, Randi Winter, David L. Williams, Chuanfu Li

ETSU Faculty Works

Serum lactate levels are traditionally interpreted as a marker of tissue hypoxia and often used clinically as an indicator of severity and outcome of sepsis/septic shock. Interestingly, recent studies involving the effects of tumor-derived lactate suggest that lactate itself may have an immunosuppressive effect in its local environment. This finding adds to the recent advances in immunometabolism that shed light on the importance of metabolism and metabolic intermediates in the regulation of innate immune and inflammatory responses in sepsis. In this article, we summarize recent studies, showing that the activation of immune cells requires aerobic glycolytic metabolism and that lactate …

2018-January-04-Graduate Research Award, University Marketing And Communications, East Tennessee State University

ETSU News

No abstract provided.

An Investigation Into Tetrodotoxin (Ttx) Levels Associated With The Red Dorsal Spots In Eastern Newt (Notophthalmus Viridescens) Efts And Adults, Mackenzie M. Spicer, Amber N. Stokes, Trevor L. Chapman, Edmund D. Brodie, Edmund D. Brodia, Brian G. Gall

ETSU Faculty Works

We investigated the concentration of tetrodotoxin (TTX) in sections of skin containing and lacking red dorsal spots in both Eastern newt (Notophthalmus viridescens) efts and adults. Several other species, such as Pleurodeles waltl and Echinotriton andersoni, have granular glands concentrated in brightly pigmented regions on the dorsum, and thus we hypothesized that the red dorsal spots of Eastern newts may also possess higher levels of TTX than the surrounding skin. We found no difference between the concentrations of TTX in the red spots as compared to neighboring skin lacking these spots in either efts or adults. However, efts with more …

The Rise Of Noncommunicable Diseases In Kenya: An Examination Of The Time Trends And Contribution Of The Changes In Diet And Physical Inactivity, Edward Michieka Onyango, Benjamin Moranga Onyango

ETSU Faculty Works

This study examined correlations of historical changes in diet and physical inactivity with the rise of noncommunicable diseases (NCDs) in Kenya. Historical data on diet, wage jobs by industry, urbanization, gross domestic product (GDP), and morbidity due to NCDs were extracted from Kenya Statistical Abstracts, Food and Agriculture Organization (FAOSTAT), and the World Bank online database. These data were plotted and correlations between these factors and the incidence of different NCDs over time were evaluated. There was a rapid rise in the incidence of circulatory disease starting in 2001, and of hypertension and diabetes starting in 2008. The rise of …

Etsu Health Annual Report - 2018, 2018

ETSU Health Annual Reports

No abstract provided.

Niche Modeling For The Genus Pogona (Squamata: Agamidae) In Australia: Predicting Past (Late Quaternary) And Future (2070) Areas Of Suitable Habitat, Julie E. Rej, T. Andrew Joyner

ETSU Faculty Works

Background: As the climate warms, many species of reptiles are at risk of habitat loss and ultimately extinction. Locations of suitable habitat in the past, present, and future were modeled for several lizard species using MaxEnt, incorporating climatic variables related to temperature and precipitation. In this study, we predict where there is currently suitable habitat for the genus Pogona and potential shifts in habitat suitability in the past and future. Methods: Georeferenced occurrence records were obtained from the Global Biodiversity Information Facility, climate variables (describing temperature and precipitation) were obtained from WorldClim, and a vegetation index was obtained from AVHRR …

Limd1 Is Induced By And Required For Lmp1 Signaling, And Protects Ebv-Transformed Cells From Dna Damage-Induced Cell Death, Ling Wang, Mary E. A. Howell, Brooke Mcpeak, Katrina Riggs, Carissa Kohne, Jether Uel Yohanon, Daniel E. Foxler, Tyson V. Sharp, Jonathon P. Moorman, Zhi Q. Yao, Shunbin Ning

ETSU Faculty Works

LIMD1 (LIM domain-containing protein 1) is considered as a tumor suppressor, being deregulated in many cancers to include hematological malignancies; however, very little is known about the underlying mechanisms of its deregulation and its roles in carcinogenesis. Epstein-Barr Virus (EBV) is associated with a panel of malignancies of lymphocytic and epithelial origin. Using high throughput expression profiling, we have previously identified LIMD1 as a common marker associated with the oncogenic transcription factor IRF4 in EBV-related lymphomas and other hematological malignancies. In this study, we have identified potential conserved IRF4- and NFκB-binding motifs in the LIMD1 gene promoter, and both are …

2017-December-20-Dr. Qian Xie, University Marketing And Communications, East Tennessee State University

ETSU News

No abstract provided.

Playing With Biofeedback: A Practical, Playful Approach To Using Biofeedback In Pediatric Health, Jason L. Steadman, Michael E. Feeney

ETSU Faculty Works

No abstract provided.

Loss Of Sympathetic Nerves In Spleens From Patients With End Stage Sepsis, Donald B. Hoover, Thomas Christopher Brown, Madeleine K. Miller, John B. Schweitzer, David L. Williams

ETSU Faculty Works

The spleen is an important site for central regulation of immune function by noradrenergic sympathetic nerves, but little is known about this major region of neuroimmune communication in humans. Experimental studies using animal models have established that sympathetic innervation of the spleen is essential for cholinergic anti-inflammatory responses evoked by vagal nerve stimulation, and clinical studies are evaluating this approach for treating inflammatory diseases. Most data on sympathetic nerves in spleen derive from rodent studies, and this work has established that remodeling of sympathetic innervation can occur during inflammation. However, little is known about the effects of sepsis on spleen …

Acute Kidney Injury, Immune Thrombocytopenic Purpura, And The Infection That Binds Them Together: Disseminated Histoplasmosis, Pooja Sethi, Jennifer Treece, Chidinma Onweni, Vandana Pai, Sowminya Arikapudi, Lakshmi Kallur, Varun Kohli, Jonathan Moorman

ETSU Faculty Works

Untreated human immunodeficiency virus (HIV) can be complicated by opportunistic infections, including disseminated histoplasmosis (DH). Although endemic to portions of the United States and usually benign, DH can rarely act as an opportunistic infection in immunocompromised patients presenting with uncommon complications such as acute kidney injury and idiopathic thrombocytopenic purpura. We report a rare presentation of DH presenting with acute kidney injury and immune thrombocytopenic purpura in an immunocompromised patient with HIV.

Prescription Drug Abuse And Provider-Patient Communication: A Qualitative Analysis Of The Perspectives Of Prescribers And Patients, Stephanie M. Mathis

Electronic Theses and Dissertations

Prescription drug abuse is a public health problem of epidemic proportions in the United States. Provider-patient communication underpins many initiatives aimed at preventing and reducing the public health burden of prescription drug abuse. The characteristics of and factors contributing to this interpersonal process, however, have not been fully explored.

The purpose of this research was to examine: 1) the overall problem of prescription drug abuse and provider-patient communication about prescription drug abuse from the patient perspective; and 2) provider-patient communication about prescription drug abuse from the prescriber perspective. In 2014-2015, semi-structured interviews were conducted with 20 patients from primary care …

Exogenous Ubiquitin: Role In Myocardial Inflammation And Remodeling Post- Ischemia/Reperfusion Injury, Stephanie Scofield

Electronic Theses and Dissertations

Sympathetic stimulation occurs in the heart after injuries such as ischemia/reperfusion (I/R) and myocardial infarction and affects myocardial remodeling. Prolonged sympathetic stimulation can result in myocardial dysfunction through its effects on cardiac myocyte apoptosis and myocardial fibrosis. Ubiquitin (UB) is well known for its role of tagging old or damaged proteins for degradation via the UB-proteosome pathway. The role of exogenous UB however, is not fully understood. Previously, our lab showed that β-adrenergic receptor (β-AR) stimulation increased levels of extracellular UB in the conditioned media of adult rat ventricular myocytes and that UB inhibits β-AR-stimulated apoptosis. This study investigates the …

The Importance Of Human Population Characteristics In Modeling Aedes Aegypti Distributions And Assessing Risk Of Mosquito-Borne Infectious Diseases, Julie F. Obenauer, T. Andrew Joyner, Joseph B. Harris

ETSU Faculty Works

Background: The mosquito Aedes aegypti has long been a vector for human illness in the Southeastern United States. In the past, it has been responsible for outbreaks of dengue, chikungunya, and yellow fever and, very recently, the Zika virus that has been introduced to the region. Multiple studies have modeled the geographic distribution of Ae. aegypti as a function of climate factors; however, this ignores the importance of humans to the anthropophilic biter. Furthermore, Ae. aegypti thrives in areas where humans have created standing water sites, such as water storage containers and trash. As models are developed to examine the …

2017 November 10 - Board Of Trustees Meeting, Board Of Trustees, East Tennessee State University

Board of Trustees Agendas and Minutes

No abstract provided.