Digital Commons Network^™

The Circulome: A Dynamic Reservoir Of Genetic Heterogeneity In Agricultural Pests, Hailey Spier Camposano

All Theses

Weed and insect pests cost the agricultural sector billions in losses each year across the world, despite distributing over 1 billion pounds of potentially harmful chemicals into agroecosystems to manage these pests. Two major agricultural pests that are rapidly adapting to multiple xenobiotics are Amaranthus palmeri and Musca domestica. A. palmeri, or Palmer amaranth, is highly productive in terms of vegetative structures and reproductive propagules. Palmer amaranth has evolved multiple resistance mechanisms to several herbicidal modes of action. House fly (M. domestica) presents issues as a vector for pathogenic microbes among livestock, poultry, and humans. This …

Habitat Selection And Breeding Ecology Of Bachman’S Sparrow (Peucaea Aestivalis) In A Wiregrass-Free Ecosystem, Mikayla Thistle

All Theses

Through much of its range, Bachman’s Sparrow (Peucaea aestivalis) uses the wiregrass (Aristida spp.) dominant understory typical of longleaf pine (Pinus palustris) forest. The central South Carolina Coastal Plain, however, lies within the “wiregrass gap” where longleaf pine understories are absent of wiregrass and instead are dominated by bluestem grasses (Schizachyrium spp. and Andropogon spp.), bracken fern (Pteridium aquilinum), and shrubs. Habitat use of Bachman’s Sparrow in this region has yet to be studied and declining Bachman’s Sparrow populations necessitate a better understanding of habitat selection processes and population dynamics …

Effects Of Invasive Wild Pigs And Supplemental Feeding On Wildlife In The Piedmont Region Of South Carolina, Elizabeth A. Saldo

All Theses

Wild pigs (Sus scrofa), which are invasive in much of the world, can alter ecosystems and compete with native species through interference competition and resource exploitation. We assessed the potential for interspecific interactions between invasive wild pigs and other wildlife in the Piedmont region of South Carolina by examining their spatiotemporal overlap in a correlational field study and an experimental field study. Our correlational study used data from wildlife cameras in seasonal occupancy, N-mixture, and temporal overlap analyses. Both deer (Odocoileus virginianus) and coyote (Canis latrans) site use were negatively associated with wild pig …

"A Beauty In The Kitchen": The Introduction Of The Cookstove As A Mechanism Of Change In Charleston's Historic Kitchens, Maria Short

All Theses

While Charleston’s historic houses have long captivated visitors, scholars, and preservationists, the architecture of these properties’ kitchens and the ways people cooked in these historic spaces have long been overlooked, in part because their historic fabric has often been obscured by later alterations or demolition. While interpretation of these historic spaces in certain house museums, such as the Nathaniel Russell House or Heyward-Washington House, now include information on the lives of the enslaved who cooked in these kitchens, the understanding of cooking technology, specifically the transition from hearth cooking to cooking on cookstoves, in Charleston remains largely unstudied. In the …

At Home In The Garden, Aviana Wells

All Theses

This series of photographs serves as an expression of my personal search and longing for a sense of at homeness in varied garden landscapes across the United States. My interest in photographing gardens is derived from personal history in family gardens and grew in my frequent visits to the South Carolina Botanical Garden. The Botanical Garden was central in my life during the first year of the COVID-19 pandemic, and it was there that I found an interest in how a shared public space could offer us a place for privacy and reflection. My photographs bear witness to the spaces …

Evaluating The Accuracy And Quality Of Microgreens Training Materials Available On The Internet: A Content Analysis, Supun Chathuranga Nabadawa Hewage

All Theses

Microgreens are the young, edible seedlings of various vegetables, spices, herbs, and considered as the intermediate stage of sprouts and mature greens, suggesting microgreens may share similar food safety risks with both of these produce. Even though there are no known outbreaks due to contaminated microgreens, multiple product recalls have been reported, indicating food safety risks associated with microgreens should not be underemphasized. A recent national survey of the U.S. microgreens industry reported that almost half of growers (48.3% of 176) learned to grow microgreens by viewing websites and videos on the internet.¹ However, it is unknown whether the …

Characterization Of A Potential Glucose Transporter In Trypanosoma Brucei, Matthew Morgan

All Theses

Trypanosoma brucei, the African trypanosome, is an organism heavily dependent on glucose for ATP production during the infectious stage of its life cycle. Here, we have explored the role of an uncharacterized protein designated “novel glucose transporter” (NGT) as a potential glucose transporter. Sequence analyses suggests that NGT shares similarities (either at the primary sequence level or structurally) with Trypanosome Hexose Transporters 1 (TbTHT1), and human GLUT3, both of which are membrane sugar transporters. NGT was localized by fluorescence microscopy to subcellular structures consistent with lysosomes. Silencing NGT expression with RNA interference in parasites resulted in a growth defect …

Pasture Biomass Estimation Using Crop Surface Modeling And Vegetation Indices, Brendan Macinnis

All Theses

Biomass estimations are a critical function of any grazing or haylage system. Determination of ideal harvesting or grazing times help optimize the quality and quantity of above-ground biomass (AGB). The objective of this study was to evaluate the use of unmanned aerial vehicles (UAVs) for Alfalfa and Tall Fescue biomass estimations. Using a DJI Mavic Pro, RGB and NDVI images were taken and used to create orthomosaic images. Structure-from-motion (SfM) techniques were used to developed digital elevation models to evaluate the change in canopy height (∆H) between pre- and post-harvests. Change in canopy height (∆H) was shown to provide the …

The Role Of Consumers In The Adoption Of Alternatively Fueled Vehicles And Mitigation Of Vulnerabilities Associated With Electric Vehicle Charging, Elaina D. Stuckey

All Theses

Electric vehicles have the potential to replace traditional automobiles as the primary form of transportation. Despite major improvements in technology and an expanding focus on climate change making electric vehicles more practical than ever before, consumers are still wary of adopting them for legitimate reasons such as costs and charging infrastructure. Therefore, a concerted effort must be made to persuade individuals and companies to adopt this beneficial technology to reduce the carbon footprint and catalyze the construction of important charging infrastructure.

Though there are a multitude of benefits to adopting electric vehicles, there will be some negative effects on the …

Quantifying Shear Stresses In Tissue Engineered Aortic Heart Valves, Raj Nitin Dave

All Theses

Present heart valve prosthesis have limitations such as capability to grow, repair and remodel post implantation. Tissue engineering offers to be a promising alternative to overcome these limitations. Maturation of seeded human cells on the valve subjected to favorable growth conditions in the bioreactor is critical to the success of tissue engineered heart valves. Mechanical stress and strain which results from the pressure and flow conditions in the bioreactor plays a critical role on the developing valve tissue and are currently unknown. The goal of this research is to relate the magnitude of wall shear stress (WSS) within the heart …

Economic Revitalization Through Preservation: A Study Of The Effects Of Preservation And Economic Initiatives In Five Small Cities In Virginia, Isabella Rae Gordineer

All Theses

Some preservation and economic based initiatives in small cities strive to be the driving force behind revitalization and economic growth. Small cities, especially those distant from major cities, experience periods of shrinkage. These places often struggle to stabilize and grow. Interventions from the local, state, and national levels can assist revitalization efforts with help from planners and redevelopment specialists. These interventions include initiatives with both preservation and economic objectives. Preservation initiatives include revolving funds, tax credits, foundations and advocacy organizations, historic districts, architectural review boards, ordinances, and the Main Street Program. Economic initiatives that include preservation principles and practices include …

Fabrication Of Hydrogel Microparticles For Acute Treatment Of Penetrating Brain Injuries, Isabell Foulger

All Theses

Traumatic brain injuries (TBIs) are a type of acquired head trauma that results from an external force to the head. The development and severity of the secondary injury increases the risk of neurodegeneration and reduced cognitive recovery. Glucocorticoids are potent antiinflammatory therapeutics that have been investigated for potential treatment of TBIs, strokes, and other neuroinflammatory pathologies. However, their clinical application has been limited due to their ability to induce a variety of side effects, including adrenal suppression, edema, and reduced plasticity of the hippocampus. The use of a localized drug delivery system would overcome these systemic concerns. In previous studies, …

The Old Harbor: A Diachronic Study Of Charleston's Cooper River Waterfront, 1884-1990, Branden Gunn

All Theses

For the better part of three centuries, Charleston’s Cooper River waterfront functioned as an important commercial seaport complete with wharves, warehouses, offices, workshops, and other related buildings. These resources defined the area for nearly three centuries, yet today, most connections to the maritime past have been severed. Revitalization efforts and modern developments have redefined the area and filled voids created by the waterfront’s steady decline throughout the 20th century. With an aim to better understand the Cooper River waterfront’s developmental history, this thesis utilized historic Sanborn Fire Insurance maps to track the harbor’s physical and spatial changes from 1884 to …

Identification Of Genetic Variants Underlying Maxillary Canine-Tooth Mesioversion And Small Body Size In Shetland Sheepdogs, Sydney R. Abrams

All Theses

The domesticated dog (Canis lupus familiaris) exhibits a unique population structure, with high levels of genetic homogeneity within breeds due to selective breeding. These closed breeding populations can result in breed-specific inherited disorders. Maxillary canine-tooth mesioversion (MCM) is a genetically complex dental anomaly observed nearly exclusively in the small Shetland Sheepdog breed. Here, we utilized a genome-wide approach to discover a locus of major effect on chromosome 9. Using whole-genome resequencing data from a bilaterally affected dog, we identified variants in two genes: FTSJ3, encoding an RNA methyltransferase, and GH1, encoding growth hormone. Independent genome-wide association …

Elucidating The Cellular Physiology Of Glyphosate Resistance In Palmer Amaranth (Amaranthus Palmeri) Using Integrated Omics Approaches, Pawanjit Kaur Sandhu

All Theses

The evolution of resistance to herbicides in weeds poses a major threat to agricultural production systems. To date, herbicide resistance has been reported against 21 modes of action in 266 weed species across 71 countries. More than 50 weed species have developed resistance against glyphosate, the most widely used herbicide worldwide. Although several mechanisms of glyphosate resistance have been discovered, our understanding of alterations in the cellular physiology of glyphosate-resistant weed biotypes, and the induction of the resistance mechanisms remains limited. This knowledge is critical to developing sustainable weed management practices and for a comprehensive understanding of plant stress adaptations. …

Use Of 14c-Pfoa To Study Uptake And Biological Effects Of Pfoa In Brassica Juncea, Bryanna Dawn Wattier

All Theses

Perfluorooctanoic acid (PFOA), a surfactant, is a member of the perfluoroalkyl acids (PFAAs) family and is a contaminant of emerging concern for human and environmental health. PFOA is a persistent organic pollutant but currently little is known about (1) the potential ecological and toxicological effects of PFOA and (2) how PFOA moves in the environment. This study employs a radiotracer (¹⁴C-PFOA) to study the uptake and effects of PFOA in hydroponically grown Brassica juncea, a representative crop species. Plants were exposed in quadruplicate over the course of seven days (with plants sampled on day 4 and day 7) …

A Novel Wearable Device For Distributed Sensing Of Elastomeric Strain Gauges, Jacob Read Garland

All Theses

Throughout the past decade, the field of wearable technology has exploded in both consumer and researcher interest, to the point that its market size is anticipated to grow by nearly 150% by 2028 [1]. Sensor-embedded devices such as the Apple Watch, Fitbit, certain smart shoe insoles, and more have become more commonplace in our daily lives. Partially due to the growth of this market, interest in conductive flexible and/or elastic materials has also increased, with fields such as printed electronics, conductive polymers, and elastomers embedded with conductive percolation networks. Although they have made their debut into foldable/rollable screens recently, flexible …

Portable U-Bent Optical Fiber Biosensor For Detecting Microcystin-Lr, Quan Dio Anh Le

All Theses

Cyanobacteria are the only known prokaryotes that can photosynthesize. Due to their unique ability, they are important entities in the global carbon and nitrogen cycles. However, two factors can turn cyanobacteria into a nuisance. In general, eutrophication is the process that supplies external nutrients that are needed for cyanobacteria blooms into bodies of water from the surrounding environment. Climate warming intensifies this process by producing carbon dioxide, methane, and nitrous oxide. With cyanobacteria blooms occurring worldwide, they are becoming a bigger threat to human health, and there has been increased interest in detecting cyanobacteria and their toxic byproducts.

For instance, …

Female Portrayal In Games Through Costumes: Designing And Creating Appealing Armor For Female Players, Nancey Fang

All Theses

This thesis details the design, development, and creation of variations of more functional female armor designed for games with the female audience in mind.

The goal is to empower female players by designing and creating strong armor that is not sexualized and that women can enjoy. I want to show that women can be portrayed as strong and powerful in games by designing armor that is equivalent to their male counterparts. Due to the gender imbalance in the video game industry and player base, it seems that female players are not catered to when most games are created. Many studies …

Rewriting The Narrative That Surrounds Mental Illness, Ashley Johnson

All Theses

The focus of this work is to change the narrative that surrounds mental health issues by portraying mental battles through animation. Buddhism heavily influenced the crux of this project, which is the conscious decision to accept oneself and choose to work towards inner balance instead of battles. 3D animation is used to distill complex thoughts into simplified characters that drive the narrative through performance and choreographed fight scenes.

Covid-19 And Canine Travelers: Determining Likelihood To Travel With Dogs, Angela Rowan

All Theses

During the Covid-19 pandemic, dog adoption rates skyrocketed, restaurants focused on outdoor seating, and travelers pivoted away from tightly packed planes out of safety concerns. This study surveyed dog owners in the United States to determine whether pet attachment levels could predict dog owners’ likelihood of traveling with their dogs. In addition, it used Um and Crompton’s (1992) facilitators and inhibitors to establish how different factors affect a dog owner’s likelihood of traveling with their dog. These facilitators and inhibitors were split into three dimensions: needs satisfaction, social agreement, and travelability. Finally, this study sought to learn what effect the …

Effects Of Prescribed Fire Seasonality On Forest Structure And Bird Populations In The Southern Appalachians, Allison Melcher

All Theses

Decades of fire suppression have contributed to the loss of historical ecosystems and to the decline of wildlife populations throughout the Southern Appalachian region. Recognizing the importance of fire in enhancing habitat and wildlife diversity, forest managers in recent years have begun implementing fire as a management tool to recover traditional disturbance regimes. Most of these burns take place during the dormant season, but some research has indicated dormant season burns are not effective in restoring ecosystem heterogeneity, and there has been a push to expand the use of fire into the growing season. However, much is still unknown about …

Agency In Tourism: A Narrative Of Reclamation Found In The Public History Of The Eastern Band Of The Cherokee Indian, Caroline M. Ross

All Theses

This thesis covers the development of the tourism industry surrounding the Eastern Band of the Cherokee Indian in Western North Carolina, specifically the evolution of the outdoor historical drama Unto These Hills written by Kermit Hunter. The chapters to follow examine the development of the production in conjunction with the broader historical context, analyze Kermit Hunter’s original script contextualizing the material and Hunter’s narrative choices, and elucidate the history and representation of one of the production’s protagonists. All of this to demonstrate not only the misrepresentation and inaccuracy of the original production, but to discuss the production’s role on the …

Co-Habitation And Migration: Urban Design For Bird Migratory Pathways, Steve C. Kurtz

All Theses

Urban landscapes are intricate systems interacting throughout the globe. Over the past decades, urban development has widely sprawled and fragmented flyway systems and corridors. This has led to many changes in species composition and has even caused the extinction of some animals (Marzluff 1997). Recent studies have indicated that there exists a protective or restorative design to create stepping-stone connectivity in urban areas. This study looks to understanding potential benefits in urban biodiversity, animal species protection, and urban benefits of green roof designs.

Connecting Process Performance And Microbial Community Dynamics During Anaerobic Wastewater Treatment To Improve Microbial Resource Management, Jessica Anne Deaver

All Dissertations

Anaerobic wastewater treatment technologies produce renewable energy and recover resources through microbial conversion of organics. Anaerobic digestion is commonly utilized for municipal wastewater sludge treatment, and co-digestion with other organic-rich substrates like fats, oils, and grease (FOG) can improve methane production. Methane can be captured for combined heat and power production. Microbial electrochemical cells (MXCs) convert organic waste to electrical current to produce electricity or other valuable chemicals. Both these technologies would benefit from understanding the microbial conversion processes more thoroughly. FOG co-digestion sometimes inhibits rather than bolsters methane production. MXCs have potential for blackwater (urine and feces) treatment in …

Fate And Transport Of Toxoplasma Gondii Oocysts In Saturated Porous Media: Effects Of Electrolytes And Natural Organic Matter, Christian Pullano

All Theses

Toxoplasma gondii is a pathogenic microorganism that is currently a threat to public health. Understanding the fate and transport of T. gondii through the soil and groundwater is vital in determining the risk it poses to water resources and human health. The physico-chemical interactions between the groundwater and the bio colloid within an aquifer will dictate its mobility and its ability to infect humans. This research examines how various naturally occurring groundwater chemistries containing organic compounds and monovalent and divalent salt solutions will alter the fate and transport of T. gondii. Solutions containing various concentrations of humic acid, fulvic …

Effects Of Tannin-Free Grain Sorghum Varieties On The Metabolizable Energy, Performance, And Intestinal Health Of Poultry, Alissa Moritz

All Dissertations

The single largest cost of poultry production is the cost of feed, and it is favorable to seek more sustainable, alternative feed ingredients to reduce the competition between human, animal, and industrial users. Grain sorghum is a potential alternative feedstuff to minimize the dependence of corn by the poultry industry. Not only is it nutritionally similar to corn, but it is tolerant to dry, sandy soils, which allows it to grow successfully in arid climates. This is highly desirable because of its potential to alleviate the production pressure of growing other cereal grains like corn that are less tolerant to …

Incorporating Human Effects In Quantifying Mechanisms Of Stream Fish Community Structure Using Metacommunity Theory, Lauren E. Stoczynski

All Dissertations

Metacommunity theory incorporates local and regional factors to understand how biotic communities are structured across the landscape. Despite established knowledge of how humans impact aquatic systems, inclusion of anthropogenic factors in metacommunity studies have been largely ignored. Additionally, alpha, beta, and gamma diversity can all be explored at the metacommunity level to investigate mechanistic drivers of community structure. Beta diversity can be further partitioned into turnover and richness difference components, each with different mechanistic drivers. Streams provide an excellent study system for metacommunity research because of the dendritic structure of watersheds and the natural delineation that watershed boundaries provide. Large-extent …

Characterization Of Mef2c-Related Disorders: Genotype, Phenotype, And Gene Pathway Dysregulation, Jessica Ann Cooley Coleman

All Dissertations

MEF2C­-related disorders are characterized by intellectual disability, developmental delay, lack of speech, seizures, stereotypic movements, hypotonia, and brain abnormalities and are caused by pathogenic alterations involving the MEF2C gene. Despite published cases, MEF2C­-related disorders are difficult to recognize clinically. These studies sought to further characterize MEF2C­-related disorders by investigating the genotypes, phenotypes, and gene functions (or dysfunctions) associated with the disorder.

Tremors have been reported in some patients with MEF2C­-related disorders, but the concept of tremors has been complicated by vague definitions and numerous categorization methods. We performed a concept analysis following the Walker and Avant …

Identification Of Genetic Factors Underlying Megaesophagus And Startle Disease In Dogs, Sarah M. Bell

All Dissertations

Dogs provide an excellent model for human hereditary disease research; thus, the development of canine genomic tools has been prioritized in recent years. Today, SNP arrays, multiple genome assemblies, and multi-breed reference panels containing whole genome resequencing (WGS) data from hundreds of canids are available to facilitate genome-wide genotyping in the dog. Herein, a variety of genome-wide techniques are employed to identify the genetic factors underlying congenital idiopathic megaesophagus (CIM) in two breeds, German shepherd dogs (GSDs) and Great Danes, and startle disease in Spanish greyhounds.

CIM is a complex canine esophageal motility disorder characterized by ineffective peristalsis and esophageal …