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Developing Transmissible Vaccines For Animal Infections: Intrinsically Safe Designs And A Staged Transparent Development Process Will Be Essential, Daniel G. Streiker, Megan E. Griffiths, Rustom Antia, Laura Bergner, Peter Bowman, Maria Vitoria Dos Santos De Moraes, Kevin Esvelt, Mike Famulare, Amy T. Gilbert, Biao He, Michael A. Jarvis, David A. Kennedy, Jennifer Kuzma, Carolyne Nasi,Iyu Wanyonyi, Christopher Remien, Tonie Rocke, Kyle Rosenke, Courtney Schreiner, Justin Sheen, David Simons, Ivet A. Yordanova, James J. Bull, Scott L. Nuismer

United States Department of Agriculture Wildlife Services: Staff Publications

Developing Transmissible Vaccines for Animal Infections: Intrinsically Safe Designs and a Staged Transparent Development Process Will Be Essential

Intrinsically safe, biologically compelling vaccine designs

Pahsed development must incorporate checkpoints and engagement

Staged development with established checkpoints

Wquitable partnerships with international governance

Toward deployment

Workshop organization

Seven proposed commitm ents for the responsible development of transmissible vaccines for infectious disease control in animals

Evolutionary outcomes of vaccine transmission depend on the viral vector and the genetic modifications introduced

Animal Health Matters, Russ Daly, Jane Hennings

Animal Health MATTERS Newsletter

[Page] 1- Porcine Epidemic Diarrhea Virus Testing and Research in High Demand at SDSU’s ADRDL [Page] 1- Clinical Signs Associated with Swine Deltacoronavirus in a Northeastern Nebraska Sow Herd [Page] 2- Director's Message: ADRDL Contributes to Animal Health Locally, Nationally, and Globally [Page] 3- Dr. Step Highlights Successful Bailey Herd Health Conference [Page] 4- Detecting and Discovering Viruses at SDSU’s ADRDL [Page] 6- Post Exposure Rabies Treatment for People Currently Rabies-Vaccinated [Page] 7- Antimicrobial Resistance the Subject of Latest South Dakota “One Health” Workshop Group Meeting [Page] 6- New Hires [Page] 6- New Hire: Grant Miller [Page] 7- Continuing Education …

Cd8Α Dendritic Cells Drive Establishment Of Hsv-1 Latency, Kevin R. Mott, Sariah J. Allen, Mandana Zandian, Bindu Konda, Behrooz G. Sharifi, Clinton Jones, Steven L. Wechsler, Terrence Town, Homayon Ghiasi

School of Veterinary and Biomedical Sciences: Faculty Publications

It is generally accepted that CD8 T cells play the key role to maintain HSV-1 latency in trigeminal ganglia of ocularly infected mice. Yet, comparably little is known about the role of innate immunity in establishment of viral latency. In the current study, we investigated whether CD8α DCs impact HSV-1 latency by examining latency in the trigeminal ganglia (TG) of wildtype (WT) C57BL/6 versus CD8α^‒/‒ (lack functional CD8 T cells and CD8α⁺ DCs), CD8β^‒/‒ (have functional CD8α⁺ T cells and CD8α⁺ DCs), and β2m^‒/‒ (lack functional CD8 T cells but have CD8α^{+ …}

Overexpression Of Thioredoxin Binding Protein (Tbp-2) Increases Oxidation Sensitivity And Apoptosis In Human Lens Epithelial Cells, Yibo Yu, Kuiyi Xing, Rilwan Badamas, Charles A. Kuszynski, Hongli Wuand, Marjorie F. Lou

School of Veterinary and Biomedical Sciences: Faculty Publications

Thioredoxin (Trx) is an important redox regulator with cytosolic Trx1 and mitochondrial Trx2 isozymes. Trx has multi-physiological functions in cells and its bioavailability is negatively controlled through active site binding to a specific thioredoxin binding protein (TBP-2). This paper describes the delicate balance between TBP-2 and Trx, and the effect of overexpression of TBP-2 in the human lens epithelial cells. Cells overexpressing TBP-2 (TBP-2 OE) showed a 7- fold increase of TBP-2, and a nearly 40% suppression of Trx activity but no change in Trx expression. The TBP-2 OE cells grew slower and their population decreased to 30% by day …

Forecasting The Spread Of Raccoon Rabies Using A Purpose-Specific Group Decision-Making Process., Aaron M. Anderson, Stephanie A. Shwiff, Richard B. Chipman, Todd C. Atwood, Tyler Cozzens, Frank Fillo, Robert Hale, Brody Hatch, Joanne Maki, Olin E. Rhodes Jr., Erin E. Rees, Charles E. Rupprecht, Rowland Tinline, Kurt C. Vercauteren, Dennis Slate

United States Department of Agriculture Wildlife Services: Staff Publications

The Centers for Disease Control (CDC) and USDA Wildlife Services (WS) have been involved in an oral rabies vaccination (ORV) program for raccoons (Procyon lotor) that has slowed the westward spread of raccoon rabies. The objective of this study was to forecast the spread of the disease if an ORV zone was not maintained. A group decision-making process was designed to address the forecasting problem and was implemented using a group of 15 experts and 4 support personnel at a meeting at the USDA National Wildlife Research Center. Ten expansion regions were constructed that described the spread of …

Evaluation Of Novel Oral Vaccine Candidates And Validation Of A Caprine Model Of Johne’S Disease, Murray E. Hines Ii, Sue E. Turnquist, Marcia R.S. Ilha, Sreekumari Rajeev, Arthur L. Jones, Lisa Whittington, John P. Bannantine, Raul G. Barletta, Yrjö T. Gröhn, Robab Katani, Adel M. Talaat, Lingling Li, Vivek Kapur

School of Veterinary and Biomedical Sciences: Faculty Publications

Johne’s disease (JD) caused by Mycobacterium avium subspecies paratuberculosis (MAP) is a major threat to the dairy industry and possibly some cases of Crohn’s disease in humans. A MAP vaccine that reduced of clinical disease and/or reduced fecal shedding would aid in the control of JD. The objectives of this study were (1) to evaluate the efficacy of 5 attenuated strains of MAP as vaccine candidates compared to a commercial control vaccine using the protocol proposed by the Johne’s Disease Integrated Program (JDIP) Animal Model Standardization Committee (AMSC), and (2) to validate the AMSC Johne’s disease go at challenge model. …

Temperature, Hypoxia, And Mycobacteriosis: Effects On Adult Striped Bass Morone Saxatilis Metabolic Performance, Dominique Lapointe, Wolfgang K. Vogelbein, Mary C. Fabrizio, David T. Gauthier, Richard W. Brill

Biological Sciences Faculty Publications

Mycobacteriosis, a chronic bacterial disease of fishes, is prevalent in adult striped bass from Chesapeake Bay (USA). Although environmental factors may play a role in disease expression, the interaction between the disease and environmental stress remains unexplored. We therefore examined the individual and interactive effects of elevated temperature, hypoxia, and mycobacteriosis on the metabolism of wild-caught adult striped bass from Chesapeake Bay using respirometry. Because the spleen is the primary target organ of mycobacteriosis in striped bass, we hypothesized that the disease interferes with the ability of fish to increase their hemato crit in the face of increasing oxygen demands. …

Nebraska Beef Quality Assurance: Focused On The Safety, Wholesomeness And Quality Of Beef

Nebraska Beef Quality Assurance Program

Across the nation, beef producers face the challenge of making a living from the land, while producing safe, wholesome beef. Beef that will provide a great eating experience each and every time for American and International consumers.

South Dakota Animal Disease Research & Diagnostic Laboratory: Annual Report 2014, Animal Disease Research And Diagnostic Laboratory, South Dakota State University

South Dakota Animal Disease Research and Diagnostic Laboratory (ADRDL)

This document is the 2014 annual report of the South Dakota Veterinary and Biomedical Science Department Animal Disease Research and Diagnostic Laboratory.

Light Stimulates The Mouse Adrenal Through A Retinohypothalamic Pathway Independent Of An Effect On The Clock In The Suprachiasmatic Nucleus, Silke Kiessling, Patricia J. Sollars, Gary E. Pickard

School of Veterinary and Biomedical Sciences: Faculty Publications

The brain’s master circadian pacemaker resides within the hypothalamic suprachiasmatic nucleus (SCN). SCN clock neurons are entrained to the day/night cycle via the retinohypothalamic tract and the SCN provides temporal information to the central nervous system and to peripheral organs that function as secondary oscillators. The SCN clock-cell network is thought to be the hypothalamic link between the retina and descending autonomic circuits to peripheral organs such as the adrenal gland, thereby entraining those organs to the day/night cycle. However, there are at least three different routes or mechanisms by which retinal signals transmitted to the hypothalamus may be conveyed …

Dens Agenesis And Cervical Vertebral Malformation In A Labrador Retriever Puppy, Elisa Salas, Henry E. Cerny, Seth P. Harris

School of Veterinary and Biomedical Sciences: Faculty Publications

Cervical vertebral malformations can cause acute or progressive pain, paralysis, and potentially death. Herein we present a case report of dens agenesis; cervical vertebral malformations of C1, C2, C6, and C7; subluxation of C1 and C2 and C4 and C5; and associated degenerative myelopathy in a 5-month-old female Labrador Retriever puppy. We additionally review current knowledge regarding pathogenesis and treatment.

Relationship Between Degree Of Anemia And Blood Gases In Cattle With Theileriosis, Mehmet Temi̇z, Nuri̇ Altuğ, Nazmi̇ Yüksek

Turkish Journal of Veterinary & Animal Sciences

This study was conducted to determine the relationship between the degrees of anemia and blood gases in cattle with theileriosis. It included 28 cattle with theileriosis and 7 healthy animals as the control group. The cattle with theileriosis were divided into 4 groups according to their hematocrit (Hct) values: cattle with Hct of >26 were considered as nonanemic (Group 1), Hct of 20-26 as mildly anemic (Group 2), Hct of 14-19 as moderately anemic (Group 3), and Hct of 10-13 as severely anemic (Group 4). The PCO_2, HCO_3, and TCO_2 levels in the study groups were lower than those of …

The Effects Of Dietary Glutamine Supplementation On Growth Performance And Intestinal Morphology Of Broiler Chickens Reared Under Hot Conditions, Farhad Jazideh, Parviz Farhoomand, Mohsen Daneshyar, Gholamreza Najafi

Turkish Journal of Veterinary & Animal Sciences

Dietary supplementation effects of 0%, 0.25%, 0.5%, and 1% glutamine were investigated on the performance and development of the gastrointestinal tract in broilers reared under hot conditions. No significant differences were observed between the treatments for feed intake and feed conversion ratio during the starter period, grower period, or entire experiment (P > 0.05). During the grower period, only 0.5% glutamine-fed birds had higher body weight gain than others (P < 0.05). For the entire period, body weight gain of 0.5% glutamine-fed birds was greater than that of 0.00% and 0.25% glutamine-fed ones (P < 0.05). Furthermore, 0.5% glutamine-fed birds showed longer villus height than other birds (P < 0.05). In the jejunum, both 0.5% and 1% glutamine consumption caused longer villus height as compared to 0.00% and 0.25% glutamine (P < 0.05). Villus height of 0.25% glutamine-fed birds was higher than that of 0.00% glutamine-fed ones (P < 0.05). None of the ileum morphological parameters were affected by glutamine supplementation (P > 0.05). In conclusion, supplementation of 0.5% glutamine improved the intestinal morphology and body weight gain of broilers under heat stress. Higher dietary glutamine (1%) increased the villus height in the jejunum …

Serological Survey Of Equid Herpesvirus 3 Infection In Turkish Horses, Veysel Soydal Ataseven, Firat Doğan, Seval Bi̇lge Dağalp, Mehmet Tolga Tan, Köksal Altinbay, Cafer Tayyar Ateş

Turkish Journal of Veterinary & Animal Sciences

Sera from 1133 Turkish horses were obtained and tested for equid herpesvirus 3 (EHV-3) antibodies using a sero-neutralisation test. The sample consisted of 420 privately owned working horses from 2 regions of Turkey, 428 brood horses from private stud farms and breeding stations, and 285 racehorses from private stud farms. Neutralising antibodies against EHV-3 were found in 287 (25.3%) of the 1133 analysed sera. The highest seroprevalence for EHV-3 was detected in the brood horses (51.2%), followed by the racehorses (10.2%), and the working horses (9.3%). The seroprevalence rates tended to be higher in female horses than in male horses …

Nmr-Based Metabolomic Evaluation In Dairy Cows With Displaced Abomasum, Abdullah Başoğlu, Nuri̇ Başpinar, Alparslan Coşkun

Turkish Journal of Veterinary & Animal Sciences

This study is the first to evaluate nuclear magnetic resonance (NMR)-based metabolomics in cows with displaced abomasum (DA), which is an internationally recognized problem in dairy cows. Some biochemical parameters have been used for monitoring DA. However, to date, few data have been available on the blood metabolomic profile of dairy cows. Forty Holstein multiparous cows with DA (30 left, 10 right) and 10 clinically healthy Holstein multiparous cows were the subjects of the study. All the animals had similar dry matter intake. An NMR-based metabolomics approach and hematological and biochemical analyses were performed. Some changes in biochemical parameters were …

Severe Beak Deformity In Melopsittacus Undulatus Caused By Knemidocoptes Pilae, Pedro María Alarcón Elbal, Victor Jesus Carmona Salido, José Marín Sánchez Murillo, Rafael Calero Bernal, Javier Lucientes Curdi

Turkish Journal of Veterinary & Animal Sciences

An outbreak of knemidocoptic mange caused by Knemidocoptes pilae is described in caged Australian budgerigars (Melopsittacus undulatus) in a public park in L´Eliana (Valencia, Spain). Five individuals (5 out of 70) displayed severe malformation of the beak, with the rhamphotheca, rhinotheca, and gnathotheca affected and a slight lateral deviation of the jawbone, which did not, however, impede feeding or grooming.

Novel Gammaherpesvirus Functions 1 Encoded By Bovine Herpesvirus (Bovine Lymphotropic Virus), J. Jia, G. Delhon, E. R. Tulman, D. G. Diel, Fernando A. Osorio, X. Wen, G. F. Kutish, D. L. Rock

School of Veterinary and Biomedical Sciences: Faculty Publications

The genus Macavirus of the subfamily Gammaherpesvirinae includes viruses that infect lymphoid cells of domestic and wild ruminants and swine, causing asymptomatic latent infections in reservoir hosts. Here, we describe the genome of bovine herpesvirus 6 (BoHV-6), a

macavirus ubiquitous in healthy cattle populations. The BoHV-6 genome exhibited architecture conserved in macaviruses, including a repetitive H-DNA region and unique, 141 kilobase pair L

DNA region predicted to encode 77 genes. BoHV-6 encoded in variable genomic regions a novel complement of genes relative to other characterized macaviruses, likely contributing to distinctive aspects of BoHV-6 infection biology and host range. Most notably, …

Role Of Viral And Host Factors In Influenza Virus Mediated Inhibition Of Interleukin-23, Ashish Tiwari

Theses and Dissertations--Veterinary Science

Influenza virus is one of the major respiratory pathogens of humans as well as animals, including equines. There is an increasing evidence that bacterial infections are the most common cause of the death during influenza. In horses also, secondary bacterial pneumonia can lead to death, and surviving horses may take up to six months for the complete recovery resulting in heavy economic loss to the equine industry. Interleukin (IL)-23 mediated innate immune response has been shown to protect the host from various respiratory bacterial infections. However, studies to investigate the role of host and viral factors in the regulation of …

Examination Of The Snsag Surface Antigen Gene Family In Sarcocystis Neurona, Ablesh Gautam

Theses and Dissertations--Veterinary Science

Sarcocystis neurona is a protozoan parasite that causes the serious neurologic disease equine protozoal myeloencephalitis (EPM). The life cycle of S. neurona progresses through multiple developmental stages that differ morphologically and molecularly. The S. neurona merozoite surface is covered by multiple related proteins, which are orthologous to the surface antigen (SAG) gene family of Toxoplasma gondii. The SAG surface antigens in T. gondii and another related parasite Neospora caninum are life cycle stage-specific and seem necessary for parasite transmission and persistence of infection. The present research was conducted to explore the gene family of SnSAGs in S. …

The Midgut Bacterial Flora Of The Hard Tick Hyalomma Anatolicum (Acari: Ixodidae) From South India As Determined By Molecular Analyses, Sankarappan Anbalagan, Vimalanathan Arun Prasanna, Mani Kannan, Muthukalingan Krishnan

Turkish Journal of Veterinary & Animal Sciences

The midgut bacterial flora of post-blood meal and starved or digested blood meal of hard ticks (Hyalomma anatolicum) from goats were analyzed using BOX PCR, ERIC PCR and 16S rDNA gene sequencing. BOX PCR and ERIC PCR showed dissimilar band patterns between microbial isolates from the midgut of the ticks. In total, 9 bacterial species belonging to 2 genera (Bacillus and Staphylococcus) were identified in the midgut of both types (post-blood meal and digested blood meal) of tick by using 16S rDNA gene sequencing. The majority of the midgut bacterial species were Staphylococcus sp. (90%) and there was a high …

Clinical Presentation And Ultrasonographic Findings In Buffaloes With Congestive Heart Failure, Hussein Awad Hussein, Rudolf Staufenbiel

Turkish Journal of Veterinary & Animal Sciences

The objective of this retrospective study was to describe the clinical presentation and ultrasonographic findings in water buffaloes suffering from congestive heart failure. Fifty-three buffaloes with congestive heart failure, which was confirmed postmortem, were included. The final diagnoses were traumatic pericarditis (n = 38) and vegetative endocarditis (n = 15). Ten healthy buffaloes were enrolled in this study as controls. The main clinical presentations were those related to right-sided congestive heart failure including peripheral edema, venous distension and jugular pulsation, and a sharp drop in milk yield. Only 5 buffaloes exhibited signs of left-sided heart failure. Ultrasonographic examinations showed cardiac …

Evaluating The Clinical And Physiological Effects Of Long Term Ultraviolet B Radiation On Guinea Pigs (Cavia Porcellus), Megan K. Watson, Adam W. Stern, Amber L. Labelle, Stephen Joslyn, Timonthy M. Fan, Katie Leister, Micah Kohles, Kemba Marshall, Mark A. Mitchell

School of Veterinary and Biomedical Sciences: Faculty Publications

Vitamin D is an important hormone in vertebrates. Most animals acquire this hormone through their diet, secondary to exposure to ultraviolet B (UVB) radiation, or a combination thereof. The objectives for this research were to evaluate the clinical and physiologic effects of artificial UVB light supplementation on guinea pigs (Cavia porcellus) and to evaluate the long-term safety of artificial UVB light supplementation over the course of six months. Twelve juvenile acromelanic Hartley guinea pigs were randomly assigned to one of two treatment groups: Group A was exposed to 12 hours of artificial UVB radiation daily and Group B …

Bovine Herpesvirus 1 Productive Infection Stimulates Inflammosome Formation And Caspase 1 Activity, Jianlin Wang, Jeffrey Alexander, Matthew S. Wiebe, Clinton J. Jones

Nebraska Center for Virology: Faculty Publications

Bovine herpesvirus 1 (BoHV-1), a significant viral pathogen of cattle, causes inflammation in affected tissue during acute infection. Consequently, we tested whether productively infected bovine cells stimulate inflammasome formation. Expression of two components required for inflammasome formation, the DNA sensor IFI16 (gamma-interferon-inducible protein 16) and NLRP3 (NOD-like receptor family, pyrin domain containing 3), were induced in bovine kidney cells by eight hours after infection. IFI16 was detected in punctate granules localized to the cytoplasm and nucleus. During productive infection, more than ten times more cells were caspase 1 positive, which is activated following inflammasome formation. Two caspase 1 inhibitors had …

Experimental Infection Of Pregnant Goats With Bovine Viral Diarrhea Virus (Bvdv) 1 Or 2, Thomas Passler, Kay P. Riddell, Misty A. Edmondson, Manuel F. Chamorro, John D. Neill, Bruce W. Brodersen, Heather L. Walz, Patricia K. Galik, Yijing Zhang, Paul H. Walz

School of Veterinary and Biomedical Sciences: Faculty Publications

Infections with bovine viral diarrhea virus (BVDV) of the genus pestivirus, family Flaviviridae, are not limited to cattle but occur in various artiodactyls. Persistently infected (PI) cattle are the main source of BVDV. Persistent infections also occur in heterologous hosts such as sheep and deer. BVDV infections of goats commonly result in reproductive disease, but viable PI goats are rare. Using 2 BVDV isolates, previously demonstrated to cause PI cattle and white-tailed deer, this study evaluated the outcome of experimental infection of pregnant goats. Pregnant goats (5 goats/group) were intranasally inoculated with BVDV 1b AU526 (group 1) or BVDV 2 …

Glutaredoxin-2 Is Required To Control Oxidative Phosphorylation In Cardiac Muscle By Mediating Deglutathionylation Reactions, Mary-Ellen Harper, Ryan J. Mailloux, Jian Ying Xuan, Skye Mcbride, Wael Maharsy, Stephanie Thorn, Chet E. Holterman, Christopher R.J. Kennedy, Peter Rippstein, Robert Dekemp, Jean Da Silva, Mona Nemer, Marjorie Lou

School of Veterinary and Biomedical Sciences: Faculty Publications

Glutaredoxin-2 (Grx2) modulates the activity of several mitochondrial proteins in cardiac tissue by catalyzing deglutathionylation reactions. However, it remains uncertain whether Grx2 is required to control mitochondrial ATP output in heart. Here, we report that Grx2 plays a vital role modulating mitochondrial energetics and heart physiology by mediating the deglutathionylation of mitochondrial proteins. Deletion of Grx2 (Grx2−/−) decreased ATP production by complex I-linked substrates to half that in wild type (WT) mitochondria. Decreased respiration was associated with increased complex I glutathionylation diminishing its activity. Tissue glucose uptake was concomitantly increased. Mitochondrial ATP output and complex I activity could be recovered …

Altered Entrainment To The Day/Night Cycle Attenuates The Daily Rise In Circulating Corticosterone In The Mouse, Patricia J. Sollars, Michael J. Weiser, Andrea E. Kudwa, Jayne R. Bramley, Malcolm D. Ogilvie, Robert L. Spencer, Robert J. Handa, Gary E. Pickard

School of Veterinary and Biomedical Sciences: Faculty Publications

The suprachiasmatic nucleus (SCN) is a circadian oscillator entrained to the day/night cycle via input from the retina. Serotonin (5-HT) afferents to the SCN modulate retinal signals via activation of 5-HT_1B receptors, decreasing responsiveness to light. Consequently, 5-HT_1B receptor knockout (KO) mice entrain to the day/night cycle with delayed activity onsets. Since circulating corticosterone levels exhibit a robust daily rhythm peaking around activity onset, we asked whether delayed entrainment of activity onsets affects rhythmic corticosterone secretion. Wheel-running activity and plasma corticosterone were monitored in mice housed under several different lighting regimens. Both duration of the light:dark cycle (T …

The Catabolite Control Protein E (Ccpe) Affects Virulence Determinant Production And Pathogenesis Of Staphylococcus Aureus, Torsten Hartmann, Grégory Baronian, Nadine Nippe, Meike Voss, Bettina Schulthess, Christiane Wolz, Janina Eisenbeis, Kerstin Schmidt-Hohagen, Rosmarie Gaupp, Cord Sunderkötter, Christoph Beisswenger, Robert Bals, Greg A. Somerville, Mathias Herrmann, Virginie Molle, Markus Bischoff

School of Veterinary and Biomedical Sciences: Faculty Publications

Carbon metabolism and virulence determinant production are often linked in pathogenic bacteria, and several regulatory elements have been reported to mediate this linkage in Staphylococcus aureus. Previously, we described a novel protein, catabolite control protein E (CcpE) that functions as a regulator of the tricarboxylic acid cycle. Here we demonstrate that CcpE also regulates virulence determinant biosynthesis and pathogenesis. Specifically, deletion of ccpE in S. aureus strain Newman revealed that CcpE affects transcription of virulence factors such as capA, the first gene in the capsule biosynthetic operon; hla, encoding α-toxin; and psmα, encoding the phenol-soluble modulin cluster α. Electrophoretic mobility …

Sumoylation Differentially Regulates Sp1 To Control Cell Differentiation, Lili Gong, Wei-Ke Ji, Xiao-Hui Hu, Wen-Feng Hu, Xiang-Cheng Tang, Zhao-Xia Huang, Ling Li, Mugen Liu, Shi-Hua Xiang, Erxi Wu, Zachary Woodward, Yi-Zhi Liu, Quan Dong Nguyen, David Wan-Cheng Li

School of Veterinary and Biomedical Sciences: Faculty Publications

The mammalian small ubiquitin-like modifiers (SUMOs) are actively involved in regulating differentiation of different cell types. However, the functional differences between SUMO isoforms and their mechanisms of action remain largely unknown. Using the ocular lens as a model system, we demonstrate that different SUMOs display distinct functions in regulating differentiation of epithelial cells into fiber cells. During lens differentiation, SUMO1 and SUMO2/3 displayed different expression, localization, and targets, suggesting differential functions. Indeed, overexpression of SUMO2/3, but not SUMO1, inhibited basic (b) FGF-induced cell differentiation. In contrast, knockdown of SUMO1, but not SUMO2/3, also inhibited bFGF action. Mechanistically, specificity protein 1 …

Protective Humoral Immune Response Induced By An Inactivated Porcine Reproductive And Respiratory Syndrome Virus Expressing The Hypo-Glycosylated Glycoprotein 5, Jung-Ah Lee, Byungjoon Kim, Fernando A. Osorio, Asit K. Pattnaik, Nak-Hyung Lee, Sang-Won Lee, Seung-Yong Park, Chang-Seon Song, In-Soo Choi, Joong-Bok Lee

School of Veterinary and Biomedical Sciences: Faculty Publications

Porcine reproductive and respiratory syndrome (PRRS) causes significant economic losses to the swine industry worldwide. Although inactivated and live vaccines are commercially available for the control of PRRS, both types of vaccine have not always proven successful in terms of generating a protective immune response, particularly in the case of inactivated vaccines. In this study, we tested whether an inactivated vaccine could induce a humoral immune response to PRRS during a homologous challenge. Amino acid substitutions were introduced into glycoprotein (GP) 5 of the FL12 strain of the PRRS virus (PRRSV) using site-directed mutagenesis with a pFL12 infectious clone. The …

Light Stimulates The Mouse Adrenal Through A Retinohypothalamic Pathway Independent Of An Effect On The Clock In The Suprachiasmatic Nucleus, Silke Kiessling, Patricia J. Sollars, Gary E. Pickard

School of Veterinary and Biomedical Sciences: Faculty Publications

The brain’s master circadian pacemaker resides within the hypothalamic suprachiasmatic nucleus (SCN). SCN clock neurons are entrained to the day/night cycle via the retinohypothalamic tract and the SCN provides temporal information to the central nervous system and to peripheral organs that function as secondary oscillators. The SCN clock-cell network is thought to be the hypothalamic link between the retina and descending autonomic circuits to peripheral organs such as the adrenal gland, thereby entraining those organs to the day/night cycle. However, there are at least three different routes or mechanisms by which retinal signals transmitted to the hypothalamus may be conveyed …