Digital Commons Network^™

Mutational Analysis Of Area, A Transcriptional Activator Mediating Nitrogen Metabolite Repression In Aspergillus Nidulans And A Member Of The “Streetwise” Gata Family Of Transcription Factors, Richard A. Wilson, Herbert N. Arst Jr.

Fungal Molecular Plant-Microbe Interactions

Details a mutational analysis of AREA, a transcriptional activator mediating nitrogen metabolite repression in Aspergillus nidulans and a member of the “streetwise” GATA family of transcription factors.

The Mortenson Ranch: Cattle And Trees At Home On The Range. A Restoration Guidebook, Susan E. Boettcher, W. Carter Johnson, Scott Kronberg, F. Robert Gartner, Clarence Todd, Jeff Mortenson, Scott Fausti

Natural Resource Management Faculty Publications

Early agriculture on western rangeland met with little success and resulted in serious consequences including soil erosion, loss of native woodlands and wildlife, and economic ruin. The Mortenson family in Stanley County, South Dakota, has been engaged in restoring degraded rangeland on their ranch for more than 50 years. Their primary goal has been to return the land to its condition prior to white settlement while maintaining a profitable cattle ranching operation. In recent years the ranch has served as a model of successful ranching based on a conservation ethic. This guidebook summarizes the restoration techniques and grazing regime used …

Biodegradation Of Atrazine By Agrobacterium Radiobacter J14a And Use Of This Strain In Bioremediation Of Contaminated Soil, J. K. Struthers, K. Jayachandran, T. B. Moorman

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

We examined the ability of a soil bacterium, Agrobacterium radiobacter J14a, to degrade the herbicide atrazine under a variety of cultural conditions, and we used this bacterium to increase the biodegradation of atrazine in soils from agricultural chemical distribution sites. J14a cells grown in nitrogen-free medium with citrate and sucrose as carbon sources mineralized 94% of 50 μg of [¹⁴C-U-ring]atrazine ml^-1 in 72 h with a concurrent increase in the population size from 7.9 x 10⁵ to 5.0 x 10⁷ cells ml^-1. Under these conditions cells mineralized the [ethyl-¹⁴C]atrazine …

Residual Effects Of Crop Residues On Grain Production And Selected Soil Properties, James F. Power, Paul Koerner, John W. Doran, Wallace Wilhelm

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Returning crop residue improves water conservation and storage, nutrient availability, and crop yields. We have little knowledge, however, of the residual impacts of crop residues on soil and crop production. We hypothesized that residual impacts of crop residues vary with the amount of residues used. A 10-yr study near Lincoln, NE, evaluated the residual effects of an earlier 8-yr study of various crop residue amounts on crop growth and selected soil properties. From 1978 through 1985, crop residues were returned at 0,50, 100, and 150% of the quantity produced by the previous crop (averaging 0 to =6 Mg ha^{-1 …}

Agricultural Water Management In The Missippi Delta Region Of Arkansas, H. Don Scott, James A. Ferguson, Linda Hanson, Todd Fugitt, Earl Smith

AAES Research Reports and Research Bulletins

Agriculture is the largest use of soil and water resources in eastern Arkansas. This bulletin summarized the recent historical use of soil and water by agriculture and the impact of irrigation on yields of rice, soybeans and cotton. The experiments conducted in the field to quantitatively schedule irrigations of crops are summarized. The results show the close relationship between the irrigation of crops and the extraction of water from the Alluvial Aquifer. The implications of this relationaship for the future are discussed.

Plans For Crash-Tested Bridge Railings For Longitudinal Wood Decks On Low-Volume Roads, Michael A. Ritter, Ronald K. Faller, David B. Bunnell, Paula D. Hilbrich Lee, Barry Thomas Rosson

United States Department of Agriculture, Forest Service / University of Nebraska-Lincoln: Faculty Publications

The plans for crashworthy bridge railings for low-volume roads were developed through a cooperative research program involving the USDA Forest Service, Forest Products Laboratory (FPL); the Midwest Roadside Safety Facility, University of Nebraska-Lincoln (MwRSF); and the Forest Service, National Forest System, Engineering. Three railings were developed and successfully tested in accordance with National Cooperative Highway Research Program (NCHRP) Report 350 Test Level-1 requirements. The fourth system was developed for a lower test level based on criteria developed by the Forest Service for single-lane bridges on very low-volume roads. For the convenience of the user, full drawing sets are provided in …

Nebline, August 1998

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Harvesting Everlasting Flowers for Drying
Watering tips
Scorch—curse of plants on the plains
New grass faces the big test
Storing extra seeds
Control of ground ivy in the lawn
Audubon purchases 610-acre O'Brien Ranch
Household hazardous waste collection
Don't get stung!
Allergic to bee stings?
Dealing with unwanted guests
How animals stay cool when it's hot!
Fruit, vegetables and freedom to farm
Prescribed burning of CRP
What is LB1209 and does it affect you?
Toxic plants
How often to pump your septic tank
Caring for animals when gone
Flies
Field Day features high-value crops
Buildings
Festival of Color
Fresh, fabulous …

A Partial Host Range Of The High Plains Virus Of Corn And Wheat, Dallas L. Seifers, Tom L. Harvey, T. J. Martin, Stanley G. Jensen

Department of Plant Pathology: Faculty Publications

Barley (Hordeum vulgare), cheat (Bromus secalinus), corn (Zea mays), oat (Avena sativa), rye (Secale cereale), and wheat (Triticum aestivum) were infected by a Kansas isolate of the High Plains virus (HPV) in greenhouse experiments, but several other grass species were not. Infection of a host was dependent upon wheat curl mite numbers. Although both green foxtail (Setaria viridis) and yellow foxtail (S. glauca) are found naturally infected by HPV, only yellow foxtail could be infected in greenhouse experiments. Field sampling (1994 to 1996) of …

Plans For Crash-Tested Wood Bridge Railings For Concrete Decks, Michael A. Ritter, Ronald K. Faller, Paula D. Hilbrich Lee, Barry Thomas Rosson, Sheila Rimal Duwadi

United States Department of Agriculture, Forest Service / University of Nebraska-Lincoln: Faculty Publications

As part of a continuing cooperative research between the Midwest Roadside Safety Facility (MwRSF); the USDA Forest Service, Forest Products Laboratory (FPL); and the Federal Highway Administration (FHWA), several crashworthy wood bridge railings and approach railing transitions have been adapted for use on concrete bridge decks. These railings meet testing and evaluation criteria outlined in National Cooperative Research Program (NCHRP) Report 350, Recommended Procedures for the Safety Performance Evaluation of Highway Features, and include a glued-laminated timber (glulam) rail, with and without a curb, at Test Level- 2 (TL-2), a glulam rail with curb at TL-4, and a glulam …

Bumelia Lanuginosa Pers., David S. Seigler, John E. Ebinger

Specimens by Name

No abstract provided.

Phoradendron Serotinum (Raf.) M.C. Johnston, David Seigler, John E. Ebinger

Specimens by Name

No abstract provided.

Aloysia Gratissima (Gillies & Hook.) Troncoso, David S. Seigler, John E. Ebinger

Specimens by Name

No abstract provided.

Phylogenetic Relationships Within The Family Potyviridae: Wheat Streak Mosaic Virus And Brome Streak Mosaic Virus Are Not Members Of The Genus Rymovirus, Drake C. Stenger, Jeffrey S. Hall, Il-Ryong Choi, Roy C. French

Department of Plant Pathology: Faculty Publications

The complete nucleotide sequence of wheat streak mosaic virus (WSMV) has been determined based on complementary DNA clones derived from the 9,384-nucleotide (nt) RNA of the virus. The genome of WSMV has a 130-nt 5&#;&#;leader and 149-nt 3&#;-untranslated region and is polyadenylated at the 3&#;&#;end. WSMV RNA encodes a single polyprotein of 3,035 amino acid residues and has a deduced genome organization typical for a member of the family Potyviridae (5&#;-P1/HC-Pro/P3/6K1/CI/6K2/VPg-NIa/NIb/CP-3&#;). Because WSMV shares with ryegrass mosaic virus (RGMV) the biological property of transmission by eriophyid mites, WSMV has been assigned to the genus Rymovirus, of which RGMV is …

Center For Grassland Studies Newsletter, Summer 1998, Volume 4, No. 3

Center for Grassland Studies: Newsletters

Contents:

Nebraska's Wetlands by Ted LaGrange,Nebraska Game and Parks Commission

Ashfall Fossil Beds: Record of an Ancient Grassland Ecosystem Preserved in Rock by Michael Voorhies, University of Nebraska State Museum

Wet Meadow Restoration at The Nature Conservancy's Speidell Tract by Chris Helzer, The Nature Conservancy

CGS Advisory Committee Tours Golf Course, Burned Grasslands, Fossil Beds and Ranch

Forage News [1998-07], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• U.K. All Commodity Field Day
• Fall Grazing School at Princeton
• Hay Seminar at the State Fair
• Native Warm Season Grass Field Day
• Forage Council Gets Grazing School Grant from Department of Agriculture
• Joint Field Day is the Cream of the Crop
• Prepare Now for Fall and Winter Pastures
• The Thirty Yard Journey
• Leaves of Grass
• Upcoming Events

Gap Analysis Of The Vegetation Of The Intermountain Semi-Desert Ecoregion, David M. Stoms, Frank W. Davis, Kenneth L. Driese, Kelly M. Cassidy, Michael P. Murray

Great Basin Naturalist

A conservation gap analysis was conducted for the Intermountain Semi-Desert ecoregion to assess the representation of land-cover types within areas managed primarily for biodiversity objectives. Mapped distributions of plant communities were summarized by land-management status categories. The total amount of land permanently protected in the ecoregion is

Winter Macroinvertebrate Communities In Two Montane Wyoming Streams, Christopher M. Pennuto, Frank Denoyelles Jr., Mark A. Conrad, Frank A. Vertucci, Sharon L. Dewey

Great Basin Naturalist

Macroinvertebrate communities were examined on 4 winter dates over a 4-yr period in 2 high-altitude Rocky Mountain streams to document overwintering assemblages potentially experiencing spring acid pulses. Taxa richness values were comparable to other published lists for alpine and montane stream systems despite the fact that most literature reflected summer collections. Mean benthic density ranged from 1406 to 19,734 organisms/m², and drift rates ranged from 0 to 1740 organisms/100 m³. Benthic collections showed higher taxa richness than drift collections while the Ephemeroptera and Plecoptera occurred in greater proportions in drift than in benthos. The Nemouridae (Plecoptera), …

Waxy Wheats: Origin, Properties, And Prospects, Robert A. Graybosch

Department of Agronomy and Horticulture: Faculty Publications

Starch amylose is synthesized through the activity of the granule-bound starch synthase (GBSS). In wheat (Triticum aestivum L. ), there are three structural genes encoding iso- forms of GBSS. Naturally occurring mutations (null alleles) resulting in the loss of one or more GBSS isoforms have recently been identified. The presence of one or two GBSS null alleles results in the production of starch with reduced amylose content. Reduced amylose wheats have been termed `partial waxy'. Wheats with three GBSS null alleles produce essentially amylose-free, or waxy, starch. Partial waxy wheats are sources of flours with optimal quality characteristics in …

Expression Of Human Lactoferrin Cdna Confers Resistance To Ralstonia Solanacearum In Transgenic Tobacco Plants, Zhanyuan Zhang, Dermot P. Coyne, Anne K. Vidaver, Amitava Mitra

Department of Plant Pathology: Faculty Publications

A construct containing a human lactoferrin cDNA was used to transform tobacco (Nicotiana tabacum) using an Agrobacterium-mediated DNA-transfer system to express this human protein in transgenic plants. Transformants were analyzed by Southern, Northern, and Western blots to determine integration of the cDNA into the plant genome and lactoferrin gene expression levels. Most transgenic plants demonstrated significant delays of bacterial wilt symptoms when inoculated with the bacterial pathogen Ralstonia solanacearum. Quantification of the expressed lactoferrin protein by enzyme-linked immunosorbent assay in transgenic plants indicated a significant positive relationship between lactoferrin gene expression levels and levels of disease …

Identification Of A Family Of Zinc Transporter Genes From Arabidopsis That Respond To Zinc Deficiency, Natasha Grotz, Tama Fox, Erin Connolly, Walter Park, Mary Lou Guerinot, David Eide

Dartmouth Scholarship

Millions of people worldwide suffer from nutritional imbalances of essential metals like zinc. These same metals, along with pollutants like cadmium and lead, contaminate soils at many sites around the world. In addition to posing a threat to human health, these metals can poison plants, livestock, and wildlife. Deciphering how metals are absorbed, transported, and incorporated as protein cofactors may help solve both of these problems. For example, edible plants could be engineered to serve as better dietary sources of metal nutrients, and other plant species could be tailored to remove metal ions from contaminated soils. We report here the …

Physiologic Specialization Of Puccinia Recondita F. Sp. Tritici In Nebraska During 1995 And 1996, J. E. Watkins, S. S. Rutledge, P. Stephen Baenziger, W. Youngquist

Department of Plant Pathology: Faculty Publications

Field samples of Puccinia recondita f. sp. tritici, collected from four wheat-growing regions in Nebraska in 1995 and from three in 1996, were characterized for virulence. Twenty virulence phenotypes were identified in 1995 and 18 in 1996. Virulence phenotypes MBR-10,18 (virulent on Lr genes, 1, 3, 3ka, 10, 11, 18, and 30) and MDR-10,18 (virulent on Lr genes 1, 3, 3ka, 10, 11, 18, 24, and 30) were the most prevalent, with each phenotype comprising 21.6% of the isolates characterized in 1995. Of the 1995 isolates, 24% were virulent on 10 or more host genes. No virulence to Lr16 and …

The Prairie Naturalist Volume 30, No.2 June 1998

The Prairie Naturalist

REPRODUCTIVE SUCCESS OF SHARP-TAILED GROUSE IN CENTRAL MONTANA ▪ K. R. Bousquet and J. J. Rotella

EFFECTS OF DROUGHT ON RECLAIMED GRASSLANDS IN WESTERN NORTH DAKOTA ▪ D. R. Kirby, L. M. Stewart, K. D. Krabbenhoft, and D. J. Nilson

MALE COMMON NIGHTHAWK USE OF GRAVEL ROADS AT NIGHT ▪ R. G. Poulin, L. D. Todd, and R. M. Brigham

RESTORATION AND STATUS OF THE RUFFED GROUSE IN KANSAS ▪ R. D. Rodgers, R. W. Wells, K. E. Church, R. L. Whiteaker, M. T. McFadden, D. S. Lekie, R. A. Bergquist, H. J. Abel, and R. D. Applegate

PITFALL TRAPPING …

Polymerase Chain Reaction Detection And Phylogenetic Characterization Of An Agent Associated With Yellow Vine Disease Of Cucurbits, Francisco J. Avila, Benny D. Bruton, Jacqueline Fletcher, J. L. Sherwood, Sam D. Pair, Ulrich Melcher

Department of Plant Pathology: Faculty Publications

Diagnosis of yellow vine disease (YVD) in cucurbits, an important disease in the south-central United States, relies on external symptom appearance, phloem discoloration, and the presence of bacterium-like organisms (BLOs) in phloem. Polymerase chain reaction (PCR) amplification of BLO nucleotide sequences was explored as a means to improve diagnostic techniques. PCR, using a primer pair based on sequences of the citrus-greening BLO, amplified a 0.15-kilobase (kb) fragment from the DNA of symptomatic plants, but not from that of asymptomatic plants. Its nucleotide sequence suggested that the DNA amplified was of prokaryotic origin. A primer pair, designed to amplify nonspecific prokaryotic …

Opportunities For Growing Short-Rotation Woody Crops In Agroforestry Practices, Gary A. Kuhn, W. J. Rietveld

Agroforestry Notes (USDA-NAC)

Technologies are readily available for growing hybrid poplars and willows in block plantings as short-rotation woody crops (SRWC) for fuel and fiber. SRWC species and technologies can also be incorporated into agricultural systems as agroforestry practices. Examples are windbreaks, living snowfences, “timberbelts”, riparian buffer strips, and wastewater treatment plantings. Fast growth, convenient propagation (see AF Note - 11), and compatibility with conventional farming methods make SRWC suitable for use in agroforestry practices to provide multiple benefits such as solid wood and wood fiber products, water quality improvement, crop and soil protection, wildlife habitat, and buffers for agricultural/community interfaces.

Establishment And Cultural Guidelines For Using Hybrid Tree Species In Agroforestry Plantings, Gary A. Kuhn, W. J. Rietveld, Don E. Riemenschneider

Agroforestry Notes (USDA-NAC)

Hybrid poplars (and willows) are well-suited for agroforestry applications in many parts of the United States (See AFN-10). Their convenient propagation and fast growth allow them to establish and provide conservation benefits more quickly. This note provides general establishment and cultural guidelines to consider when using hybrid poplar cuttings for agroforestry plantings.

An Investigation Of Crystalline Intensity Of The Wood Of Poplar Clones Grown In Jiangsu Province, China, Bernard R. Parresol, Fuliang Cao

Aspen Bibliography

No abstract provided.

Management Of Microbial Nitrate Production In Agricultural Soils, Wei Shi

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Nitrate (NO₃^-) is of central importance in the internal soil nitrogen (N) cycle. While animal wastes and nitrification inhibitors have been used in modern agriculture for decades, their effects on soil NO₃^- concentrations in relation to microbial NO₃^- production have not been well characterized. The objective of this research was to determine microbial NO₃^- production in relation to ammonium (NH₄⁺) availability and nitrifier population activity in agricultural soils receiving animal wastes or nitrification inhibitors.

Several laboratory and field studies assessed the impacts of variously treated dairy wastes and …

Differentiation Of F18ab+ From F18ac+ Escherichia Coli By Single-Strand Conformational Polymorphism Analysis Of The Major Fimbrial Subunit Gene (Feda), Brad T. Bosworth, Evelyn A. Dean-Nystrom, Thomas A. Casey, Holly L. Neibergs

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Toxin-producing Escherichia coli expressing F18 fimbriae colonizes the small intestines of weaned pigs and causes diarrhea, edema disease, or both. The F18 family is composed of two antigenic variants, F18ab and F18ac. Because many strains do not express F18 fimbriae in vitro, identification and differentiation of these two variants are difficult. Single-strand conformational polymorphism (SSCP) analysis is a rapid method for identifying genetic mutations and polymorphisms. The F18 major fimbrial subunit genes (fedA) of 138 strains were amplified by PCR, and genetic differences were detected by SSCP analysis. The SSCP analysis of the fedA gene differentiated F18ab^{+ …}

Utilization Of Spatially Distributed Soil Resources By Several Species Common To The Great Basin, Sarah Duke

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Heterogeneous spatial and temporal distributions of soil resources important to plant growth have been documented in the sagebrush steppe ecosystem. There can exist as much variability in soil resources within the root zone of individual plants as exists across an entire field. The objective of this dissertation research was to evaluate how plants respond to, utilize and influence the spatial heterogeneity of soil resources. The three specific sets of questions addressed are outlined in the three main chapters of this dissertation.

My first study addressed how the number and concentration of phosphorus (P) patches in the root zone of an …

Perceived Fairness And Effectiveness Of Rangeland Collaborative Processes, Kimberly J. Richardson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Involvement in collaborative partnerships in natural resource management has become a popular method for natural resource management agencies to collect public input, cope with conflicts, and develop ecosystem management plans. This thesis evaluates various collaborative processes, emphasizing multiple-owner partnerships. Qualitative interviews of 46 landowners in Utah were conducted to reveal concerns and suggestions regarding multiple-owner landscape-level collaborative partnerships. Landowners were concerned about private property rights infringement and losing control of their private land. Landowners were primarily concerned about the fairness and effectiveness of any partnership in which they were involved.

However, they were willing to consider participating if certain procedural …