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Elaeagnus Angustifolial. As A Biomonitor Of Heavy Metal Pollution, Ahmet Aksoy, Uğur Şahi̇n Jan 1999

Elaeagnus Angustifolial. As A Biomonitor Of Heavy Metal Pollution, Ahmet Aksoy, Uğur Şahi̇n

Turkish Journal of Botany

The leaves of Elaeagnus angustifolia L. (Elaeagnaceae) were tested as a possible biomonitor of heavy metal pollution in Kayseri. Concentration of Pb, Cd and Zn were determined in unwashed and washed leaves and soils. Differences between the unwashed and washed samples varied according to the metal pollutant levels. Significant correlations were obtained between the heavy metal concentrations in surface soil and washed leaf samples. E. angustifoliawas found to be a useful biomonitor of the heavy metals investigated.


Callus Production And Plant Regeneration From Anther Culture Of Some Turkish Barley Cultivars, Çi̇ğdem Savaşkan, Ivona Szarejko, M. Ci̇hat Toker Jan 1999

Callus Production And Plant Regeneration From Anther Culture Of Some Turkish Barley Cultivars, Çi̇ğdem Savaşkan, Ivona Szarejko, M. Ci̇hat Toker

Turkish Journal of Botany

Anther culture of four different barley cultivars ( Hordeum vulgareL. cvs. Anadolu, Cumhuriyet-50, Obruk-86 and Tokak-157/37) currently cultivated in Turkey has been investigated. Two different culture media (BAC3 and FHG) were used and the effect of a 21-day cold pretreatment was studied. Androgenesis on BAC3 medium was evaluated statistically in all genotypes and the percentage of anther response and the frequency of calli production were found significantly different (P


The Use Of Rapd To Detect Genetic Variation In Populations Of Liatris Aspera Michx., Duane Joseph Kitchen Jan 1999

The Use Of Rapd To Detect Genetic Variation In Populations Of Liatris Aspera Michx., Duane Joseph Kitchen

Dissertations and Theses @ UNI

The tall grass prairie is one of the most degraded and fragmented ecosystems on earth. Interest in conserving, restoring, and reconstructing tallgrass prairie began early in the 1900s, and has increased dramatically since the 1960s. Early restoration and reconstruction efforts relied primarily on seed collected from prairie remnants near the planting site, but with the increase in prairie planting efforts came a greater demand for large quantities of affordable, viable seed of native prairie species. Following an increase in the use of cultivated varieties (cultivars) of seed, concerns over the loss of genetic integrity of local populations and low genetic …


Prof.Dr. Hüsnü Demi̇ri̇z (1920-1999), Turhan Baytop Jan 1999

Prof.Dr. Hüsnü Demi̇ri̇z (1920-1999), Turhan Baytop

Turkish Journal of Botany

No abstract provided.


New Floristic Records For The Grid Squares C7 And C8 In Turkey From The Fabaceae, A. Selçuk Erteki̇n Jan 1999

New Floristic Records For The Grid Squares C7 And C8 In Turkey From The Fabaceae, A. Selçuk Erteki̇n

Turkish Journal of Botany

No abstract provided.


B.R. Wells Rice Research Studies 1997, R. J. Norman, T. H. Johnston Jan 1999

B.R. Wells Rice Research Studies 1997, R. J. Norman, T. H. Johnston

Arkansas Agricultural Experiment Station Research Series

No abstract provided.


Rhodospirillum Rubrum Poly-Β-Hydroxyalkanoate Synthase, Thomas E. Clemente, Ganish M. Kishore, Timothy A. Mitsky, David M. Stark Dec 1998

Rhodospirillum Rubrum Poly-Β-Hydroxyalkanoate Synthase, Thomas E. Clemente, Ganish M. Kishore, Timothy A. Mitsky, David M. Stark

Center for Plant Science Innovation: Faculty Publications

Isolated DNA fragments encoding a Rhodospirillum rubrum (ATCC 25903) polyhydroxyalkanoate (PHA) synthase, or biologically functional equivalents thereof, are provided. Also provided is the deduced amino acid sequence of the R. rubrum PHA synthase. These molecules are useful in the production of PHAs in bacteria and plants.


Promotor (Flt) For The Full-Length Transcript Of Peanut Chlorotic Streak Caulimovirus (Pclsv) And Expression Of Chimeric Genes In Plants, Indu B. Maiti, Robert J. Shepherd Dec 1998

Promotor (Flt) For The Full-Length Transcript Of Peanut Chlorotic Streak Caulimovirus (Pclsv) And Expression Of Chimeric Genes In Plants, Indu B. Maiti, Robert J. Shepherd

Kentucky Tobacco Research and Development Center Faculty Patents

The isolation, modification and use of wild-type and modified viral FLt promoters of peanut chlorotic streak caulimovirus (PClSV) in the expression of chimeric genes in plant cells. The FLt promoter from PClSV has been modified to have duplicated enhancer domains.


Central Crops And Soils Research Station Highmore, South Dakota: Annual Progress Report, 1998, Agricultural Experiment Station Dec 1998

Central Crops And Soils Research Station Highmore, South Dakota: Annual Progress Report, 1998, Agricultural Experiment Station

Agricultural Experiment Station and Research Farm Annual Reports

This report of the Central Crops and Soils Research Station at Highmore, South Dakota is a progress report and, therefore, the results presented are not necessarily complete nor conclusive. Any interpretation given is tentative because additional data from continuation of these experiments may produce conclusions different from those of any one year. The data presented in this report reflect the 1998 growing season.


Bulletin No. 36: Amphibians And Reptiles Of The Connecticut College Arboretum, Jill Devito, Joseph Markow Dec 1998

Bulletin No. 36: Amphibians And Reptiles Of The Connecticut College Arboretum, Jill Devito, Joseph Markow

Bulletins

No abstract provided.


The Prairie Naturalist Volume 30, No.4 December 1998 Dec 1998

The Prairie Naturalist Volume 30, No.4 December 1998

The Prairie Naturalist

FISH COMMUNITY AND WATER QUALITY CHANGES IN THE BIG SIOUX RIVER ▪ D. Dieterman, and C. R. Berry, Jr.

EFFECTS OF PARASITOIDS AND COMPETITION ON CLUTCH SIZE OF A GALLING MIDGE ▪ D. J. Kinateder and S. J. Stein

RESPONSE OF BREEDING SEASON BLUE-WINGED TEAL TO DECOY TRAPPING ▪ P. R. Garrettson

COMPARISON OF POINT-COUNT AND WADE-FLUSH METHODS FOR COUNTING DUCKS ▪ G. M. Linz, C. P McMurl, H. J. Homan, and D. L. Bergman

Reviewers 1998

Author Index - Volume 30

Subject Index - Volume 30

Announcements


Southeast South Dakota Experiment Farm Annual Progress Report, 1998, Agricultural Experiment Station Dec 1998

Southeast South Dakota Experiment Farm Annual Progress Report, 1998, Agricultural Experiment Station

Agricultural Experiment Station and Research Farm Annual Reports

This thirty-eighth annual report of the research program at the Southeast South Dakota Experiment Farm has special significance for those engaged in agriculture and the agriculturally related businesses in the nine county area of southeast South Dakota. Reports in this document include information on: temperatures and precipitation data, corn production and performance, soybean research and planting, soil testing, alfalfa yield test, fertilizer testing, herbicide research, crop rotation, sorghum, small grains, livestock research, and pest and weed control.


Nebline, December 1998 Dec 1998

Nebline, December 1998

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Holiday legends,traditions & fun
Gardening resolutions
Keep trees fresh
Cyclamen care
Painting with plants
Pruning vines and groundcovers
Combing—a non-toxic method that can eliminate head lice
Our Christmas tree
A mouse in the house
A “must read” book
It’s a hit–20,000 hits!
Use and care of home humidifiers
Deadline near for requesting free inspection in compliance with Livestock Waste Management Act
Private applicators training
Crop Protection Clinic
The Power of a PTO
Beef home study course begins fifth year
Landlords need to reduce cash crop rental rates for '99
Developing your marketing plan
Chain saw operation
Commercial handling of fresh …


Genetic Variation In Chrysopsis Floridana Small, The Endangered Florida Golden Aster, As Revealed By Random Amplification For Polymorphism Detection (Rapd), Laurie Lysle Walker Markham Nov 1998

Genetic Variation In Chrysopsis Floridana Small, The Endangered Florida Golden Aster, As Revealed By Random Amplification For Polymorphism Detection (Rapd), Laurie Lysle Walker Markham

USF Tampa Graduate Theses and Dissertations

Chrysopsis fioridana Small (Asteraceae) is a federally endangered plant endemic to the Tampa Bay area of west central Florida. It is confined to the sand pine scrub community, growing in open, sunny, and sandy areas or in disturbed areas at the edges of scrub. As a means ofassessing the genetic variability ofthis species, eight populations in Hillsborough County were compared with a seed stock collection housed at Bok Tower Gardens in Lake Wales using random amplification for polymorphism detection (RAPD). The objectives of this study were to (I) describe the overall genetic variation among and within populations and subpopulations of …


Field Scale Variability Of Nitrogen And Δ15n In Soil And Plants, D. E. Clay, J. Chang, S. A. Clay, M. Ellsbury, C. G. Carlson, D. D. Malo, D. Woodson, T. Desttur Nov 1998

Field Scale Variability Of Nitrogen And Δ15n In Soil And Plants, D. E. Clay, J. Chang, S. A. Clay, M. Ellsbury, C. G. Carlson, D. D. Malo, D. Woodson, T. Desttur

Agronomy, Horticulture and Plant Science Faculty Publications

Understanding the factors that influence soil and plant nitrogen (N) spatial variability may improve our ability to develop management systems that maximize productivity and minimize environmental hazards. The objective of this study was to determine the field (65 ha) scale spatial variability of N and δ15N in soil and corn (Zea mays). Soil, grain, and stover samples were collected from grids that ranged in size from 30 by 30 m to 60 by 60 m. Plant samples, collected following physiological maturity in 1995, were analyzed for total N and δ15N. Soil samples, collected prior …


Nebline, November 1998 Nov 1998

Nebline, November 1998

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Auntie Bacteria's holiday advice
Gifts for the gardener
Forcing bulbs
Attracting birds with the right plants
Rodent damage
Winter care for strawberry plants
Dealing with head lice? Even for experts, it's a head scratcher
Good reasons to recycle aluminum
Milk carton bird feeder
Migrating birds face many hazards
Achieving cleaner indoor air
Holding wet corn with aeration
Grain storage information on the web
Pesticide container recycling a success
Farm finances tight? Fertilize with biosolids and save $$
Acreage reply
Native prairie preservation
United States using less water
Reduce orchard woes
Mealtime tips for the preschool child
Healthy Eating: Holiday Turkey …


Comparisons Of Soil Physical Characteristics In Long-Term Tillage Winter Wheat-Fallow Tillage Experiments, L. N. Mielke, Wallace Wilhelm Nov 1998

Comparisons Of Soil Physical Characteristics In Long-Term Tillage Winter Wheat-Fallow Tillage Experiments, L. N. Mielke, Wallace Wilhelm

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Soil physical characteristics resulting from tillage of fallow-wheat (Triticurn aestivurn L.) cropping systems were compared for two soils in western Nebraska. The soil physical environment influences the amount of water entering soil and the microenvironment that influences soil biological processes important to plant response. Effects of tillage on physical properties varied with soil type and depth of soil tillage. Generally, the 0-76 mm surface layer has the largest number of physical properties that differ as a result of tillage; however, only a few properties differed at greater depths. The Alliance silt loam (fine silty, mixed, mesic, Aridic Arguistoll) soil …


Potential Of The Entomopathogenic Fungus, Metarhizium Anisopliae (Metsch.) Sorokin For Control Of The Legume Flower Thrips, Megalurothrips Sjostedti (Trybom) On Cowpea In Kenya, S. Ekesi, N. K. Maniania, K. Ampong-Nyarko, I. Onu Nov 1998

Potential Of The Entomopathogenic Fungus, Metarhizium Anisopliae (Metsch.) Sorokin For Control Of The Legume Flower Thrips, Megalurothrips Sjostedti (Trybom) On Cowpea In Kenya, S. Ekesi, N. K. Maniania, K. Ampong-Nyarko, I. Onu

All Peer-Reviewed Publications

Field experiments were conducted at Mbita, western Kenya for two seasons to evaluate the potential of the entomopathogenic fungus, Metarhizium anisopliae (Metsh.) Sorokin, for biological control of the legume flower thrips, Megalurothrips sjostedti (Trybom), on cowpea. An ultra-low-volume (ULV) oil/aqueous formulation and a high-volume (HV) aqueous formulation of conidia were applied three times each at two concentrations of 1 x 1011 and 1 x 1013 conidia ha-1. Compared with the untreated controls, both formulations significantly reduced thrips populations and plant damage in both seasons. Adult insects collected from fungal-treated plots and reared in the laboratory showed a high mortality due …


Bird Use Of Riparian Vegetation Along The Truckee River, Califonia And Nevada, Suellen Lynn, Michael L. Morrison, Amy J. Kuenzi, Jennifer C. C. Neale, Benjamin N. Sacks, Robin Hamlin, Linnea S. Hall Oct 1998

Bird Use Of Riparian Vegetation Along The Truckee River, Califonia And Nevada, Suellen Lynn, Michael L. Morrison, Amy J. Kuenzi, Jennifer C. C. Neale, Benjamin N. Sacks, Robin Hamlin, Linnea S. Hall

Great Basin Naturalist

The Truckee River in California and Nevada is subject to diverse water regimes and a corresponding variety of flow rates. Original riparian vegetation has been altered by these variable flow rates and by a variety of human uses resulting in loss of native riparian vegetation from its historic extent. We conducted bird surveys along the Truckee River during spring 1993 to (1) determine relationships between birds and the present vegetation; (2) determine the importance of different vegetation types to sensitive bird species that have declined recently in the western United States due to competition from exotic plant species, cowbird ( …


The Pseudomonas Syringae Pv. Tomato Hrpw Protein Has Domains Similar To Harpins And Pectate Lyases And Can Elicit The Plant Hypersensitive Response And Bind To Pectate, Amy O. Charkowski, James R. Alfano, Gail Preston, Jing Yuan, Sheng Yang He, Alan Collmer Oct 1998

The Pseudomonas Syringae Pv. Tomato Hrpw Protein Has Domains Similar To Harpins And Pectate Lyases And Can Elicit The Plant Hypersensitive Response And Bind To Pectate, Amy O. Charkowski, James R. Alfano, Gail Preston, Jing Yuan, Sheng Yang He, Alan Collmer

Center for Plant Science Innovation: Faculty Publications

The host-specific plant pathogen Pseudomonas syringae elicits the hypersensitive response (HR) in nonhost plants and secretes the HrpZ harpin in culture via the Hrp (type III) secretion system. Previous genetic evidence suggested the existence of another harpin gene in the P. syringae genome. hrpW was found in a region adjacent to the hrp cluster in P. syringae pv. tomato DC3000. hrpW encodes a 42.9-kDa protein with domains resembling harpins and pectate lyases (Pels), respectively. HrpW has key properties of harpins. It is heat stable and glycine rich, lacks cysteine, is secreted by the Hrp system, and is able to elicit …


Center For Grassland Studies Newsletter, Fall 1998, Volume 4, No. 4 Oct 1998

Center For Grassland Studies Newsletter, Fall 1998, Volume 4, No. 4

Center for Grassland Studies: Newsletters

Contents:

Subirrigated Meadow Management Pratices by Jerry Volesky, West Central Research and Extension Center, UNL

Wildlife Habitat Incentive Program by Craig Derickson and Gerald Jasmer, USDA, Natural Resources Conservation Service

The Effect of Planting Date on Turfgrass Seed Production in the High Plains by Rebecca Harms, Turfgrass Project Coordinator, UNL

Beef Home Study Courses Begin Fifth Year

Plan Now to Attend SRM/AFGC Joint Meeting


Morphogenesis Of Douglas Fir Buds Is Altered At Elevated Temperature But Not At Elevated Co2, Martha E. Apple, Melissa S. Lucash, David M. Olszyk, David T. Tingey Oct 1998

Morphogenesis Of Douglas Fir Buds Is Altered At Elevated Temperature But Not At Elevated Co2, Martha E. Apple, Melissa S. Lucash, David M. Olszyk, David T. Tingey

Environmental Science and Management Faculty Publications and Presentations

Global climatic change as expressed by increased CO2 and temperature has the potential for dramatic effects on trees. To determine what its effects may be on Pacific Northwest forests, Douglas-fir (Pseudotsuga menziesii ) seedlings were grown in sun-lit controlled environment chambers at ambient or elevated (+4°C above ambient) temperature, and at ambient or elevated (+200 ppm above ambient) CO2. In 1995–1996 and 1996–1997, elevated CO2 had no effect on vegetative bud morphology, while the following unusual morphological characteristics were found with greater frequency at elevated temperature than at ambient: rosetted buds with reflexed and loosened outer …


Vegetation Analysis: A Graphical Analysis Of Plant Succession In Desert Communities Affected By Fire, Jeff Lantow Oct 1998

Vegetation Analysis: A Graphical Analysis Of Plant Succession In Desert Communities Affected By Fire, Jeff Lantow

UNLV Theses, Dissertations, Professional Papers, and Capstones

The Mojave Desert is affected by fire every year. With each fire comes the removal of old growth and, in its place, new growth – consisting primarily of those species which thrive in disturbed areas. The focus of my research is to look at plant communities that have been disturbed by fire, and examine the successional pathway of these disturbed environments. The seven environments I analyzed were burned within the last twenty years and are found in the Coleogyne ramosissima ecotone throughout the Spring Mountain range near Las Vegas, Nevada. The data was collected with randomly chosen circle plots in …


Can Cover Crops Reduce Leafhopper Abundance In Vineyards?, Kent M. Daane, Michael J. Costello Sep 1998

Can Cover Crops Reduce Leafhopper Abundance In Vineyards?, Kent M. Daane, Michael J. Costello

Horticulture and Crop Science

In 3 of 4 vineyards we studied, late-season leafhopper density was lower on vines in cover cropped plots than in plots with no cover crops. However, the level of leafhopper reduction (about 15%) was rarely economically important and the mechanisms leading to reduction were not clear. For example, there were few differences in the number of leaf hopper predators or parasitoids on the vines in cover cropped versus no cover plots. However, there were significant between-treatment differences in vine growth. Plots with seasonwide maintenance of a cover crop and resident grasses had a reduction in vine vigor. Lower vine vigor …


Diversity And Dominant Species Of Ground Beetle Assemblages (Coleoptera: Carabidae) In Crop Rotation And Chemical Input Systems For The Northern Great Plains, Michael M. Ellsbury, Janine E. Powell, Frank Forcella, W. David Woodson, Sharon A. Clay, Walter E. Riedell Sep 1998

Diversity And Dominant Species Of Ground Beetle Assemblages (Coleoptera: Carabidae) In Crop Rotation And Chemical Input Systems For The Northern Great Plains, Michael M. Ellsbury, Janine E. Powell, Frank Forcella, W. David Woodson, Sharon A. Clay, Walter E. Riedell

Agronomy, Horticulture and Plant Science Faculty Publications

Dominant carabid species present in crops and crop rotation sequences commonly used in the northern Great Plains were assessed as an initial step toward the management of carabids as natural control agents. Ground beetle populations were determined by pitfall trapping in 4 crop rotation treatments maintained under high, managed, and low levels of chemical fertilizer and pesticide inputs. Diversity and species richness among crops, rotations, and input levels were compared using 3 indices—the Shannon-Weaver Index, relative diversity, and the Hierarchical Richness Index (HRI). Four carabid species, Cyclotrachelus altemans (Casey), Poecilvs lucublandus Say, Harpalns pensylvanicus (DeGeer), and Bembidion quadrimaculatum L., comprising …


Diversity And Dominant Species Of Ground Beetle Assemblages (Coleoptera: Carabidae) In Crop Rotation And Chemical Input Systems For The Northern Great Plains, Michael M. Ellsbury, Janine E. Powell, Frank Forcella, W, David Woodson, Sharon A. Clay, Walter E. Riedell Sep 1998

Diversity And Dominant Species Of Ground Beetle Assemblages (Coleoptera: Carabidae) In Crop Rotation And Chemical Input Systems For The Northern Great Plains, Michael M. Ellsbury, Janine E. Powell, Frank Forcella, W, David Woodson, Sharon A. Clay, Walter E. Riedell

Agronomy, Horticulture and Plant Science Faculty Publications

Dominant carabid species present in crops and crop rotation sequences commonly used in the northern Great Plains were assessed as an initial step toward the management of carabids as natural control agents. Ground beetle populations were determined by pitfall trapping in 4 crop rotation treatments maintained under high, managed, and low levels of chemical fertilizer and pesticide inputs. Diversity and species richness among crops, rotations, and input levels were compared using 3 indices—the Shannon-Weaver Index, relative diversity, and the Hierarchical Richness Index (HRI). Four carabid species, Cyclotrachelus altemans (Casey), Poecilvs lucublandus Say, Harpalns pensylvanicus (DeGeer), and Bembidion quadrimaculatum L., comprising …


Ecological Evaluation For The West Ash Creek Potential Research Natural Area, Pine Ridge Ranger Unit, Nebraska National Forest, Steven B. Rolfsmeier Sep 1998

Ecological Evaluation For The West Ash Creek Potential Research Natural Area, Pine Ridge Ranger Unit, Nebraska National Forest, Steven B. Rolfsmeier

Nebraska Game and Parks Commission: White Papers, Conference Presentations, and Manuscripts

The West Ash Creek Potential Research Natural Area is located within pastures 6, 7, and 11 of the Pine Ridge Unit of the Nebraska National Forest ca 18 mi. (30 km) southwest of Chadron, Nebraska. The area consists of ca 1020 acres of mostly successional pine forest burned in a 1985 wildfire, with unburned forest, some grassland, and riparian canyon bottoms. The area is within the Pine Ridge ecosystem of the White River watershed in northwestern Nebraska. The area has remained ungrazed nearly continuously since 1986.

This document was prepared to provide the Forest Service with information to determine the …


The Prairie Naturalist Volume 30, No. 3 September 1998 Sep 1998

The Prairie Naturalist Volume 30, No. 3 September 1998

The Prairie Naturalist

GROWTH OF SPOTIED BASS IN KANSAS STREAMS AND IMPOUNDMENTS ▪ J. S. Tillma and C. S. Guy

SIZE SELECTION OF RAINBOW SMELT BY SMALL MESH GILL NETS ▪ C. E. Freiburger and W. G. Duffy

ABUNDANCE AND HABITAT AFFINITIES OF Cyclotracheius substriatus (Coleoptera: Carabidae) ON NORTHERN SHORTGRASS PRAIRIE ▪ N. E. Mcintyre 157

THE REPRODUCTIVE BIOLOGY OF CANDLE ANEMONE ▪ B. Molano-Flores and S. D. Hendrix

NOTES

Noteworthy Records of Bats from Southern Colorado ▪ E. W. Valdez

Eggshell Removal by Duck Hens Following

Partial Nest Depredation by Striped Skunk ▪ S. Lariviere and L. R. Walton

New Records of …


Interactions Between The Structural Domains Of The Rna Replication Proteins Of Plant-Infecting Rna Viruses, Erin K. O'Reilly, Zhaohui Wang, Roy C. French, C. Cheng Kao Sep 1998

Interactions Between The Structural Domains Of The Rna Replication Proteins Of Plant-Infecting Rna Viruses, Erin K. O'Reilly, Zhaohui Wang, Roy C. French, C. Cheng Kao

Department of Plant Pathology: Faculty Publications

Brome mosaic virus (BMV), a positive-strand RNA virus, encodes two replication proteins: the 2a protein, which contains polymerase-like sequences, and the 1a protein, with N-terminal putative capping and C-terminal helicase-like sequences. These two proteins are part of a multisubunit complex which is necessary for viral RNA replication. We have previously shown that the yeast two-hybrid assay consistently duplicated results obtained from in vivo RNA replication assays and biochemical assays of protein-protein interaction, thus permitting the identification of additional interacting domains. We now map an interaction found to take place between two 1a proteins. Using previously characterized 1a mutants, a perfect …


A Sensitive Elisa For Pythium Ultimum Using Polyclonal And Species-Specific Monoclonal Antibodies, G. Y. Yuen, J. Q. Xia, C. L. Sutula Sep 1998

A Sensitive Elisa For Pythium Ultimum Using Polyclonal And Species-Specific Monoclonal Antibodies, G. Y. Yuen, J. Q. Xia, C. L. Sutula

Department of Plant Pathology: Faculty Publications

A double-antibody sandwich indirect enzyme-linked immunosorbent assay (ELISA) was developed for the detection and quantification of Pythium ultimum. A polyclonal antibody produced to cell walls of P. ultimum was used as the capture antibody, while a P. ultimum-specific monoclonal antibody (MAb E5) was used for recognition of the fungus. In the ELISA, culture extracts of 7 isolates of P. ultimum exhibited strong positive reactions, whereas none of the 37 isolates of other Pythium spp. and fungal genera had positive reactions. P. ultimum was detected by ELISA in roots of bean, cabbage, and sugar beet seedlings grown in pathogen-infested …