Digital Commons Network^™

Dnak And The Heat Stress Response Of Pseudomonas Syringae Pv. Glycinea, Lisa M. W. Keith, James E. Partridge, Carol L. Bender

Department of Plant Pathology: Faculty Publications

The dnaK gene from Pseudomonas syringae pv. glycinea PG4180 was cloned and sequenced. The dnaK coding region was 1,917 bp and contained a putative O³² heat shock promoter 86 bp upstream of the translational start site. grpE, another heat shock gene, was found immediately upstream of the putative dnaK promoter. The predicted amino acid sequence of dnaK showed relatedness to the ATPase and substrate binding domains commonly found in heat shock proteins, as well as the highly conserved signature sequence motifs belonging to the Hsp70 protein family. Furthermore, the PG4180 dnaK gene complemented an Escherichia coli dnaK mutant …

Bulletin 4369 - Ord River Irrigation Area - Kununurra Western Australia, Agriculture Western Australia

Bulletins 4000 -

The Ord River Irrigation Area (ORIA) in the Kimberley Province of Western Australia is the product of a bold and imaginative plan to develop part of the nation's tropical north for intensive agriculture by harnessing the waters of the Ord River. The principal features of the scheme are:

* A major storage reservoir formed by a dam constructed where the Ord River enters the Carr-Boyd Ranges, 160 kilometres south-east of Wyndham.

* A diversion dam, 50 kilometres further downstream, to raise the river level and divert water to be released from the major storage reservoir onto the irrigable lands.

* …

Morphogenesis Is Coordinated With Nuclear Division In Germinating Aspergillus Nidulans Conidiospores, Steven D. Harris

Department of Plant Pathology: Faculty Publications

Germinating Aspergillus nidulans conidiospores switch to polarized apical growth following an initial period of isotropic expansion. At the same time, they re-enter the nuclear division cycle. The relationship between spore polarization and nuclear division was investigated by testing the effect of cell cycle inhibitors and temperature-sensitive cell cycle mutations on spore morphogenesis. On rich media, it was found that spore polarization is delayed if completion of the first mitosis is blocked. The observed delay may be dependent upon the activity of the mitosis-promoting NIMA kinase. An additional mechanism appears to prevent polarization as the spore progresses through its first S …

Reproductive Biology Of Melilotus Officinalis (L.) Lam (Fabaceae: Papilionoideae): Morphology, Seed/Ovule Ratios And Ovule Abortion Patterns, Susan Lee Schouten

Dissertations and Theses

Melilotus officinalis, an introduced herbaceous species within the Papilionoideae, has a fixed ovule abortion pattern. In its primarily single-seeded, indehiscent fruits, with an average of 6.98 ovules/fruit, this species matured the basal-most ovule in 67.63% and the second basal-most ovule in 24.22% of the fruits studied. This pattern contrasts with other legumes studied, which abort the basal-most ovules or those at either end of the fruit. Workers propose interaction between two opposing gradients--that of maternal resources from the basal end of the fruit and of fertilization from the stylar end. Several lines of evidence indicate that all ovules are …

Isolated Spinach Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase Large Subunit ΕN-Methyltransferase And Method Of Inactivating Ribulose-1,5-Bisphosphatase Carboxylase/Oxygenase Large Subunit ΕN-Methyltransferase Activity, Robert L. Houtz

Horticulture Faculty Patents

The gene sequence for ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) large subunit (LS) .sup.ε N-methyltransferase (protein methylase III or Rubisco LSMT) from a plant which has a des(methyl) lysyl residue in the LS is disclosed. In addition, the full-length cDNA clones for Rubisco LSMT are disclosed. Transgenic plants and methods of producing same which have the Rubisco LSMT gene inserted into the DNA are also provided. Further, methods of inactivating the enzymatic activity of Rubisco LSMT are also disclosed.

The Prairie Naturalist Volume 31, No.2 June 1999

The Prairie Naturalist

SPATlAL VARIATION IN POPULATION CHARACTERISTICS OF SHOVELNOSE STURGEON IN THE KANSAS RIVER ▪ M. C. Quist and C. S. Guy

INFLUENCE OF FIRE AND TRAPPING EFFORT ON GROUND BEETLES IN A RECONSTRUCTED TALLGRASS PRAIRIE ▪ K. J. Larsen and J. B. Williams

FISH ASSEMBLAGES AND HABITAT RELATIONSHIPS IN A SMALL NORTHERN GREAT PLAINS STREAM ▪ C. A. Barfoot and R. G. White

CHRISTMAS BIRD COUNTS FOR NORTH DAKOTA 1998 ▪ R. N. Randall

NEW RECORDS OF THE PYGMY SHREW IN SOUTHEAST SOUTH DAKOTA ▪ C. B. King, G. M. Wilson, and P. D. Sudman

BUFFLEHEAD BROOD IN NORTHEASTERN SOUTH DAKOTA …

An Engineered Closterovirus Rna Replicon And Analysis Of Heterologous Terminal Sequences For Replication, Satyanarayana Tatineni, Siddarame Gowda, V. P. Boyko, Maria R. Albiach-Marti, Munir Mawassi, J. Navas-Castillo, Alexander V. Karasev, V. Dolja, Mark E. Hilf, D. J. Lewandowski, P. Moreno, Moshe Bar-Joseph, S. M. Garnsey, William O. Dawson

Department of Plant Pathology: Faculty Publications

Citrus tristeza virus (CTV) populations in citrus trees are unusually complex mixtures of viral genotypes and defective RNAs developed during the long-term vegetative propagation of the virus and by additional mixing by aphid transmission. The viral replication process allows the maintenance of minor amounts of disparate genotypes and defective RNAs in these populations. CTV is a member of the Closteroviridae possessing a positive-stranded RNA genome of ≈20 kilobases that expresses the replicase-associated genes as an ≈400-kDa polyprotein and the remaining 10 3' genes through subgenomic mRNAs. A full-length cDNA clone of CTV was generated from which RNA transcripts capable of …

Novel Application Of Phastsystem Polyacrylamide Gel Electrophoresis Using Restriction Fragment Length Polymorphism ± Internal Transcribed Spacer Patterns Of Individuals For Molecular Identification Of Entomopathogenic Nematodes, Horolma Pamjav, Dimitra Triga, Zsuzsanna Buzµs, Tibor Vellai, Attila Lucskai, Byron Adams, Alexander P. Reid, Ann Burnell, Christine Griffin, Itamar Glazer, Michael G. Klein, Andras Fodor

Department of Plant Pathology: Faculty Publications

A relatively rapid and economic way of identifying and assigning nematodes to taxons, which had already been determined either by comparative sequence analysis of nuclear rDNA internal transcribed spacer (ITS) region or by other methods of molecular or conventional taxonomy, is provided. Molecular identification of entomopathogenic nematodes (EPN) can be upgraded by basing it on PhastSystem polyacrylamide gel electrophoresis (PAGE) analysis of restriction fragment length polymorphism (RFLP) patterns of polymerase chain reaction (PCR)-amplified DNA derived from single nematodes of Steinernema or Heterorhabditis spp. Although analysis from single worms has previously been made on agarose gel, the resolution on PhastSystem PAGE …

Field And Laboratory Evaluation Of Soil Quality Changes Resulting From Injection Of Liquid Sewage Sludge, S. Stamatiadis, John W. Doran, Timothy A. Kettler

Department of Agronomy and Horticulture: Faculty Publications

Soil quality changes resulting from repeated or single injection of liquid municipal sewage sludge were evaluated in terraced cropland in eastern Nebraska, USA. Differences in soil properties among sampling locations were explained primarily by two factors, landscape position and sludge injection. Selected chemical properties (pH, EC, NO ₃-N) did not generally differ between landscape positions, but soil organic matter (organic C and N) and microbial activity indices (soil respiration, biomass N, available N, mineralization and nitrification rates) were more sensitive indicators of change. Values of these indicators generally increased down-slope from the upper terrace to the grassed waterway on …

Ecological Intensification Of Cereal Production Systems: Yield Potential, Soil Quality, And Precision Agriculture, Kenneth G. Cassman

Department of Agronomy and Horticulture: Faculty Publications

Wheat (Triticum aestivum L.), rice (Oryza sativa L.), and maize (Zea mays L.) provide about two-thirds of all energy in human diets, and four major cropping systems in which these cereals are grown represent the foundation of human food supply. Yield per unit time and land has increased markedly during the past 30 years in these systems, a result of intensified crop management involving improved germplasm, greater inputs of fertilizer, production of two or more crops per year on the same piece of land, and irrigation. Meeting future food demand while minimizing expansion of cultivated area primarily …

Genetic Variation In Geographical Populations Of Western And Mexican Corn Rootworm, A. L. Szalanski, R. L. Roehrdanz, D. B. Taylor, L. Chandler

Department of Plant Pathology: Faculty Publications

Genetic variation in the nuclear rDNA ITS1 region of western corn rootworm, Diabrotica virgifera virgifera (WCR), and Mexican corn rootworm, D. v. zeae (MCR) was studied. Two sites were detected which differentiated WCR and MCR in the 642-base sequence. Polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) analysis of the first internal transcribed spacer region (ITS1) sequence revealed no variation within or among the twelve WCR and two MCR populations. PCR-RFLP of 75% of the mitochondrial DNA genome detected one significant polymorphic site out of the approximately 190 restriction sizes observed in WCR. The polymorphism did not differentiate geographical populations of …

Nebline, May 1999

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

“Welcome to the Real World”
Perennials with interesting flowers
Sweet potatoes
Gardening for children
Use baits for sweet-loving ants
Dandelions: bad or good?
Did you know?
Clean water: do you take it for granted?
What are rain forests?
Pesticide container recycling program
Nitrogen can increase soybean yields
Controlled traffic in fields
Tips to wash pesticide-contaminated clothing
Poison ivy – an irritating plant
Tractor safety tips (part 5)
Butterfly gardening
WIC (Women Infants and Children) celebrates 25 years
Healthy Eating: Variable Vegetable Strata
The disappearing lunch hour
You CAN gain weight eating low fat foods!
Focus on Food
Family & Community …

Sucrose Metabolism In Two Saccharum Species, Monica Ida Valdez-Garza

Theses and Dissertations - UTB/UTPA

S. officinarum is a thick-stemmed sugarcane which stores large amounts of sucrose, and S. spontaneum is thin-stemmed with very little sucrose. Sucrose synthase (SS), sucrose phosphate synthase (SPS), and invertase enzymes are thought to affect the accumulation of sucrose in the stems of sugarcane. This study was performed to determine if (i) differences between SPS and acid invertase activities account for the difference in sucrose content of sugarcane stems, and (ii) differences in SS activity influence sucrose concentration. The difference between SPS and acid invertase activities did not appear related to the low sucrose content in S. spontaneum. However, the …

Effects Of Safflower (A Spring Crop), And Wheat Planting Date On Controlling Jointed Goatgrass (Aegilops Cylindrica) In Winter Wheat, Caleb Dale Dalley

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

To improve management and control of jointed goatgrass (Aegilops cylindrica Host.) on traditional winter wheat (Triticum aestivum L.) cropland, a better understanding of the effects spring crop and wheat planting date have on weed populations and wheat yield is needed. A study of the effects of safflower (a spring crop) and wheat planting dates (early vs late) was conducted over a 2-yr period. Long term effects will be examined over a 5-yr period. The effects these treatments had on yield, weed seed contamination, jointed goatgrass population density, and soil seedbank concentration were measured. Two identical experiments were initiated, …

Influence Of Grazing, Fire, And Rainfall Regime On Plant Species Dynamics In An Ethiopian Perennial Grassland, Michael J. Jacobs

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The dominant habitat-type of Omo National Park (ONP), Ethiopia, is grassland. This grassland supports a variety of wild herbivores and indigenous people; the latter hunt large herbivores for subsistence or graze their domestic livestock in the Park. Therefore, an understanding of grassland dynamics is a high priority for ONP management. Grazing and fire are major factors influencing species composition and vegetation change in East African grasslands. Rainfall regime, in turn, can influence both grassland response to grazing and fire. The ONP grasslands occur along a rainfall gradient ranging from subhumid to semiarid. Research objectives were to: (1) test the Milchunas, …

Ultraviolet-B Radiation: Effects On Pollen Of 34 Taxa, And Inheritance Patterns And Carryover Of Radiation Response In Arabidopsis, Javad Torabinejad

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Although considerable research has addressed effects of elevated ultraviolet-B (UV-8) radiation on vegetative plant structures and processes, the reproductive biology and patterns of inheritance of UV-B tolerance have received much less attention. I examined the effects of UV-B radiation on pollen of 34 taxa. I also addressed questions concerning the patterns of inheritance of UV-B tolerance in Arabidopsis thaliana and examined potential cumulative carryover effects of UV-B exposure through multiple generations of this species.

In the first study, a significant reduction in pollen germination occurred in only five species, but pollen tube growth in more than half of the species …

Uptake And Transformation Of Trichloroethylene By Hybrid Poplar: Laboratory Studies, Julie K. Chard

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Trichloroethylene (TCE) was widely used as an industrial solvent and degreasing agent for most of the twentieth century. It is now a widespread groundwater contaminant. Phytoremediation may be a cost-effective cleanup method for TCE-contaminated soils and groundwater. Studies of environmental TCE fate are complicated by its volatility. The literature reports both significant and insignificant plant uptake of TCE. Conflicting findings may be due to differences in exposure level, conditions, and duration of the studies, or to experimental artifacts from laboratory systems.

This research quantified plant uptake and volatilization of TCE using a unique laboratory system. Hybrid poplar trees were exposed …

Influence Of Crop Rotation, Tillage, And Management Inputs On Weed Seed Production, George O. Kegode, Frank Forcella, Sharon Clay

Agronomy, Horticulture and Plant Science Faculty Publications

Approaches to crop production that successfully reduce weed seed production can benefit farming systems by reducing management inputs and costs. A 5-yr rotation study was conducted in order to determine the effects that interactions between crop rotation, tillage, and amount of herbicide and fertilizer (management inputs) have on annual grass and broad-leaved weed seed production and fecundity. There were 10 crop rotation and tillage system combinations and three levels of management inputs (high, medium, and low). Green and yellow foxtail were the major weed species, and together they yielded between 76 and 93% of collected weed seeds. From 1990 to …

Development And Reproduction Of A Population Of Eretmocerus Eremicus (Hymenoptera: Aphelinidae) On Bemisia Argentifolii (Homoptera: Aleyrodidae), David H. Headrick, Thomas S. Bellows, Jr., Thomas M. Perring

Horticulture and Crop Science

Although the aphelinid parasitoid Eretmocerus eremicus Rose & Zolnerowich is the most abundant naturally occurring parasitoid of Bemisia argentifolii Bellows & Perring in the U.S. desert southwest, its effectiveness in different cropping systems varies. Development and reproduction of a population of this parasitoid attacking B. argentifolii infesting cotton, Gossypium hirsutum L., and sweet potato, Ipomoea batatas L., were quantified and compared. Females potato; there were no significant differences of these parameters between host plant species. A preoviposition period of 0.61 d was recorded, and a maximum number of eggs laid in a day was 69 on cotton and 13 on …

Center For Grassland Studies Newsletter, Spring 1999, Volume 5, No. 2

Center for Grassland Studies: Newsletters

Contents:

Spring Creek Prairie Near Lincoln Provides Outdoor Classroom Other Preserved Virgin Prairies in Southeast Nebraska

Algae Control in Ponds With Barley Bales: On-site Results in Nebraska by Roch Gaussoin, Department of Horticulture, UNL

CGS Offers New Grazing Livestock Systems Major

Grassland Ecology Theme of Fall 1999 CGS Seminar Series

Fair 2000 Symposium Sets Food Animal Research Priorities

Executive Order on Invasive Species

Forage News [1999-04], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Kentucky Alfalfa Conference to Celebrate 20^TH Anniversary
• Correct Web Address for Variety Testing Publications
• Spring Into Kentucky Grazing Schools
• Kentucky Forage & Grassland Council President’s – Corner
• Lime Coating Affects
• How Many Alfalfa Plants Are Needed?
• Pasture Aeration Tools
• Fence and Water Systems for Management-Intensive Grazing
• Upcoming Events

Farm And Home Research: 50-2, Larry Tennyson, Jerry Leslie, Jaimi Reimer, Stephanie Misar

Farm and Home Research: 1999 - 2007

In this Issue:

[Page] 2- Director’s comments

[Page] 3- President’s comments

[Page] 4- ‘Hope’: Edgar McFadden’s legacy: a bountiful harvest and bread for the world

[Page] 8- Break the sell-cheap, buy-high syndrome: State yearly loses millions in wages and other incomes by shipping out raw commodities

[Page] 10- Lessons and labs: Students jump-start their careers by working in SDSU labs

[Page] 12- Our ‘helping hands’ : A salute to technicians, students, secretaries—the research work crew

[Page] 14- Forewarned to forearmed: In climatology, knowing the past is key to predicting the future

[Page] 17- Wheat streak mosaic virus: In normal year, …

Real-Time Monitoring Of Escherichia Coli O157:H7 Adherence To Beef Carcass Surface Tissues With A Bioluminescent Reporter, Gregory R. Siragusa, Kevin Nawotka, Stanley D. Spilman, Pamela R. Contag, Christopher H. Contag

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

A method for studying bacteria that are attached to carcass surfaces would eliminate the need for exogenous sampling and would facilitate understanding the interaction of potential human food-borne pathogens with food animal tissue surfaces. We describe such a method in which we used a bioluminescent reporter strain of Escherichia coli O157:H7 that was constructed by transformation with plasmid pCGLS1, an expression vector that contains a complete bacterial luciferase (lux) operon. Beef carcass surface tissues were inoculated with the bioluminescent strain, and adherent bacteria were visualized in real time by using a sensitive photon-counting camera to obtain in situ …

Horticulture Research Report 1994 - 1998, Frank Wise Institute, Kununurra, Department Of Agriculture, Western Australia, D. Robinson, M. Howes, R. Randell

Horticulture research reports

This report summarises horticultural research, development and extension activities undertaken by staff from the Kununurra District Office during the period 1993 to 1998 and reported annually at the Frank Wise Institute Horticultural Research Review. The range and extent of activities presented and supported by Agriculture Western Australia with industry collaboration indicates the importance of horticulture in the economies of the Kimberley Region and Western Australia. Further support by funding bodies and agribusiness, and collaboration with other research organizations is also acknowledged. Since the early 1980's, horticultural output and value of production from the Ord River Irrigation Area (ORIA) have risen …

The Prairie Naturalist Volume 31, No.1 March 1999

The Prairie Naturalist

NEST SITE CHARACTERISTICS OF RING-NECKED PHEASANTS IN EASTERN SOUTH DAKOTA ▪ J. R. Purvis, A. E. Gabbert, and L. D. Flake

FIELD EVALUATION OF RADIOTRANSMITTERS FOR NORTHERN POCKET GOPHERS ▪ G. W. Witmer and M. J. Pipas

DISTRlBllTiON OF A MOlJND-BUILDING ANT ON NATIVE AND RESTORED PRAIRIES IN NORTHEASTERN KANSAS ▪ J. Foster and W. D. Kettle

RESULTS OF A WILD TURKEY RELEASE IN KANSAS TALLGRASS PRAIRIE ▪ J. F. Cully, Jr., P. R. Lemons, II, and R. D. Applegate

BODY-SIZE AND AGE'-RELATED MASTICATORY RELATIONSHIPS IN TWO SPECIES OF BLARINA ▪ B. J. Verts, L. N. Carraway, and R. A. …

David Grandy's Review Of David Philip Miller And Peter Hanns Reill's Visions Of Empire: Voyages, Botany, And Representations Of Nature, David Grandy

Pacific Studies

No abstract provided.

Switchgrass Seedling Development As Affected By Seed Size, Alexander J. Smart, Lowell E. Moser

Department of Agronomy and Horticulture: Faculty Publications

Seed size has been associated with early seedling vigor (i.e., germination rate, emergence rate, and growth) in grasses. This study was conducted to compare seedling development over a 60-d period in the field as affected by heavy seed (HS) (0.19 to 0.21 g 100 seed^-1) and light seed (LS) (0.13 to 0.16 g 100 seed^-1) of 'Blackwell' and 'Trailblazer' switchgrass (Panicum virgatum L.). The experiment was conducted in 1995 and 1996 at Lincoln, NE, on a Kennebec silt loam (fine-silty, mixed, superactive, mesic Cumnlic Hapludolls). The experimental design was a randomized complete block with four …

Herbicide Evaluation In Arkansas Rice, 1997, Ron Talbert, Ford Baldwin, David Gealy, Eric Webster, Tomilea Dillon, Lance Schmidt, Jeff Rutledge, Celeste Wheller, Leopoldo Estorninos Jr.

Arkansas Agricultural Experiment Station Research Series

Weed control is economically important for production of rice, a major crop in Arkansas. These findings summarize efforts of the team of Arkansas scientists working on weed control strategies for rice during 1997. Various technologies were evaluated in field studies at five locations involving the major weed problems and rice production systems used in the state. Results from these studies will add to the arsenal of weed control options for producers. Highlights include synergists and safeners for herbicides to aid in control of propanil-resistant barnyardgrass; herbicides and flooding techniques for control of red rice and other weeds; and the use …

Cloning And Developmental Expression Of Pea Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase Large Subunit Epsilon N-Methyltransferase, Robert L. Houtz

Horticulture Faculty Patents

The gene sequence for ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) large subunit (LS) .sup.ε N-methyltransferase (protein methylase III or Rubisco LSMT) is disclosed. This enzyme catalyzes methylation of the ε-amine of lysine-14 in the large subunit of Rubisco. In addition, a full-length cDNA clone for Rubisco LSMT is disclosed. Transgenic plants and methods of producing same which (1) have the Rubisco LSMT gene inserted into the DNA, and (2) have the Rubisco LSMT gene product or the action of the gene product deleted from the DNA are also provided. Further, methods of using the gene to selectively deliver desired agents to a plant …

Exploring Cost Constraints On Stem Elongation In Plants Using Phenotypic Manipulation, Don Cipollini, Jack C. Schultz

Biological Sciences Faculty Publications

Negative associations between individual life‐history traits of an organism are referred to as life‐history trade‐offs (Stearns 1992; Zera et al. 1998). The existence of costly trade‐offs is thought to have favored the evolution of phenotypic plasticity as a mechanism through which organisms can account for environmental heterogeneity while modulating costs and benefits incurred by fixed allocation to competing functions (Bradshaw 1965; Stearns 1992; Sultan 1995; Dudley and Schmitt 1996; Pigliucci 1996). In plants, the ability to modify stem elongation in response to environmental cues appears to be a classic form of adaptive phenotypic plasticity (Sultan 1995; Gedroc et al. 1996). …