Digital Commons Network^™

Efficacy Evaluation Of Foliar Fungicide Application Timing In Field Corn In Nebraska, 2012, C. M. Schleicher, T. A. Jackson-Ziems

Department of Plant Pathology: Faculty Publications

A foliar fungicide efficacy trial was conducted at the University of Nebraska-Lincoln South Central Agricultural Laboratory near Clay Center, NE. DeKalb corn hybrid DKC 64-83, rating of “good” (6 out of 9) for gray leaf spot (GLS), “very good” (4 out of 9) for common rust (CR), and “good” (5 out of 9) for southern rust (SR), was planted on 26 Apr in 30-in. rows at a target population of 30,600 plants/A. The trial area was disked with corn as the previous year’s crop. Six foliar fungicide treatments and a non-treated control were replicated six times in a randomized complete …

What’S New In Plant Pathology, Loren Giesler, Tamra A. Jackson-Ziems, Casey Schleicher, Kevin Korus

Department of Plant Pathology: Faculty Publications

Extension Plant Pathology Team Update

Plant and Pest Diagnostic Clinic Update

Disease Management Products

Table 1. Foliar Fungicide Label Updates

Table 2. New Products

Impacts Of Drought On Disease Development And Management, Stephen Wegulo, Loren Giesler, Robert Harveson, Tamra A. Jackson-Ziems, Bo Liu, Kevin Korus

Department of Plant Pathology: Faculty Publications

Drought conditions such as those that occurred in Nebraska in 2012 can impact the development of plant diseases. In general drought slows down or prevents the development of plant diseases caused by pathogens that thrive under moist conditions. However, some diseases are favored by drought. This is because when plants are stressed due to lack of moisture or excessive heat, they become more susceptible to these diseases. This article presents examples of diseases of agronomic crops favored by drought and how to manage them. Data are provided on the effect of dry or wet conditions on the profitability of applying …

Grain Storage Management To Minimize Mold And Mycotoxins, Thomas W. Dorn, Tamra A. Jackson-Ziems

Department of Plant Pathology: Faculty Publications

As most dryland corn producers are aware, the dry and hot growing season in 2012 resulted in reduced corn yields with moderately lower test weights. Along with the reduced test weights are concerns about potential mycotoxin contamination in the drought-stressed grain.

The only way to know for sure if there are mycotoxins in your grain and which specific mycotoxins are present is to collect representative grain samples and have them tested by a certified laboratory.

Many species of fungi can cause ear rot diseases and molding of grain. Most of these fungi become associated with the grain in the field …

A Virus-Encoded Potassium Ion Channel Is A Structural Protein In The Chlorovirus Paramecium Bursaria Chlorella Virus 1 Virion, Giulia Romani, Adrianna Piotrowski, Stefan Hillmer, James Gurnon, James L. Van Etten, Anna Morani, Gerhard Thiel, Brigitte Hertel

James Van Etten Publications

Most chloroviruses encode small K+ channels, which are functional in electrophysiological assays. The experimental finding that initial steps in viral infection exhibit the same sensitivity to channel inhibitors as the viral K+ channels has led to the hypothesis that the channels are structural proteins located in the internal membrane of the virus particles. This hypothesis was questioned recently because proteomic studies failed to detect the channel protein in virions of the prototype chlorovirus Paramecium bursaria chlorella virus 1 (PBCV-1). Here, we used a mAb raised against the functional K+ channel from chlorovirus MA-1D to search for the viral K+ channel …

Potassium Ion Channels: Could They Have Evolved From Viruses?1[W], Gerhard Thiel, Anna Moroni, Guillaume Blanc, James L. Van Etten

James Van Etten Publications

Cells communicate among themselves by electrical activity. Sophisticated membrane-embedded proteins, called ion channels, catalyze rapid, selective, and regulated ion fluxes across membranes (Hille, 2001). The resulting membrane currents are responsible for neuronal activity and the systemic propagation of electrical signals in animals. The activity of some channels is important for muscle movement in animals or growth in plants; other channels sense the concentration of physiological signals and modulate key processes in all kinds of eukaryotic cells. Among the many diverse ion channels in higher organisms, K+ channels are among the most important. One feature of K+ channels is that they …

Tnt1 Retrotransposon Mutagenesis: A Tool For Soybean Functional Genomics, Yaya Cui, Shyam Barampuram, Minviluz G. Stacey, C. Nathan Hancock, Seth Findley, Melanie Mathieu, Zhanyuan Zhang, Wayne A. Parrott, Gary Stacey

Faculty Publications

Insertional mutagenesis is a powerful tool for determining gene function in both model and crop plant species. Tnt1, the transposable element of tobacco (Nicotiana tabacum) cell type 1, is a retrotransposon that replicates via an RNA copy that is reverse transcribed and integrated elsewhere in the plant genome. Based on studies in a variety of plants, Tnt1 appears to be inactive in normal plant tissue but can be reactivated by tissue culture. Our goal was to evaluate the utility of the Tnt1 retrotransposon as a mutagenesis strategy in soybean (Glycine max). Experiments showed that the …

Morphometric Study Of Variation In The Genus Galeopsis (Lamiaceae), Michael T. Hughes

Masters Theses

The genus Galeopsis L. of the family Lamiaceae, also known as hemp-nettle, grows naturally in Europe and Asia, with four species naturalized in North America. The number of real species is in doubt, because of conflict over whether certain taxa are species, subspecies, or synonyms. The genus consists of five to eight taxa. I studied flower and leaf size ratios to clarify the distinctions of taxa in Galeopsis. These data were analyzed statistically, using cluster analysis, Principal Components Analysis (PCA), and Analysis of Variance (ANOVA). The results indicated that although some measurements, such as leaf width in G. angustifolia, …

Cover Page

Aliso: A Journal of Systematic and Floristic Botany

No abstract provided.

Index

Aliso: A Journal of Systematic and Floristic Botany

No abstract provided.

Evaluating The Sustainability Of Four Organic Vegetable Production Systems, Delia W. Scott

Theses and Dissertations--Plant and Soil Sciences

A field study evaluating the sustainability of four organic vegetable production systems was conducted in Lexington, Kentucky in 2006 and 2007. The four systems included no-till, raised beds covered with biodegradable black mulch, bare ground with shallow cultivation, and bare ground with shallow cultivation and wood chip mulch. The two-year study compared yield, weed control, labor, and costs associated with each system, as well as physical, chemical, and microbiological soil characteristics. In 2006, tomatoes (Lycopersicon esculentum Mill.) were grown in the four systems, with no significant difference in yield. Summer squash (Cucurbita pepo L.) was grown in the …

Aspen Status Report And Recommendation For The Book Cliffs, Paul C. Rogers, Cody M. Mittanck, Ronald J. Ryel

Aspen Bibliography

Regionally quaking aspen (Populus tremuloides Michx.) forests are experiencing numerous impediments to resilience. In the West, recent drought, fire suppression, insects, diseases, climate trends, inappropriate management, and ungulate herbivory are impacting these high biodiversity forests. We conducted a landscape assessment of aspen communities in the Book Cliffs region, Vernal Field Office of the Bureau of Land Management, for the purposes of determining landscape-level status of aspen and making recommendations for future management of these forests. The study area consists of 268 distinct aspen polygons totaling approximately 70 ha (174 acres) of aspen forest surrounded by much larger tracts of …

Book Cliffs Roadless Area Aspen Study 2013 : Grand & Uintah Counties, Utah, Cody M. Mittanck

Aspen Bibliography

No abstract provided.

Threshold Responses Of Forest Birds To Landscape Changes Around Exurban Development, Todd R. Lookingbill, Marcela Suarez-Rubio, Scott Wilson, Peter Leimgruber

Geography and the Environment Faculty Publications

Low-density residential development (i.e., exurban development) is often embedded within a matrix of protected areas and natural amenities, raising concern about its ecological consequences. Forest-dependent species are particularly susceptible to human settlement even at low housing densities typical of exurban areas. However, few studies have examined the response of forest birds to this increasingly common form of land conversion. The aim of this study was to assess whether, how, and at what scale forest birds respond to changes in habitat due to exurban growth. We evaluated changes in habitat composition (amount) and configuration (arrangement) for forest and forest-edge species around …

A Land Data Assimilation System (Ldas) Based Dataset For Regional Agro-Climatic Assessments, Xing Liu

Open Access Theses

This study is part of a USDA sponsored project ----Useful to Usable (U2U): "Transforming Climate Variability and Change Information for Cereal Crop Producers". The broader objective includes improving farm resilience and profitability in the U.S. Corn Belt region by transforming existing climate/weather data into usable knowledge and tools for the agricultural community.

The specific tasks of this research are: (1) Build a high-resolution (4 km, daily) agro-climatic dataset using a Land Data Assimilation System (LDAS). (2) Estimate regional corn yield across the Corn Belt with crop models and the agro-climatic dataset. (3) Evaluate the impacts of climate variability due to …

Slurry Seeding Cover Crops, Edwin Alexander Suarez

Open Access Theses

Cover crops improve soil quality through increased organic matter, biological activity, aggregate stability, water infiltration, and nutrient cycling. Slurry seeding, the co-application of cover crop seed and swine (Sus domesticus) manure, is a new practice that saves fuel, time and labor. High salinity and/or ammonia, among other components in swine manure, may reduce cover crop seed germination and limit the use of slurry seeding. Ten cover crop species were evaluated to compare the effects of swine manure components on cover crop seed germination. Seed germination percentages and rates were affected by swine manure but not by equivalent salinity levels established …

Developing White Ash With Resistance To The Emerald Ash Borer, Kaitlin Joy Palla

Open Access Theses

Hypocotyls isolated from mature embryos germinated on Murashige and Skoog (MS) medium supplemented with 22.2 µM 6-benzyladenine (BA) and 0.5 µM thidiazuron (TDZ) were transformed using Agrobacterium tumefaciens strain EHA105 harboring the pBI121-8D2 vector containing the neomycin phosphotransferase (nptII) gene and a full length, codon-optimized Bacillus thuringiensis Cry8Da protoxin gene (cry8D2). Explants were transformed in a bacterial suspension with 100 µM acetosyringone using 90 s sonication and 10 min vacuum-infiltration. Four independent lines of transformed shoots were selectively regenerated on MS medium with 22.2 µM BA, 0.5 µM TDZ, 50 mg L^-1 adenine sulfate, 10% …

Effects Of Selected Pesticides On Calico Scale And Its Natural Enemies, Carlos Roberto Quesada Machigua

Open Access Theses

Calico scale (Eulecanium cerasorum) is an exotic pest of shade and ornamental trees. It feeds on phloem sap, reduces tree vigor and can ultimately kill trees. We observed effects of four foliar applied (bifenthrin, pyriproxyfen, chlorantraniliprole and cyantraniliprole) and two soil applied pesticides (dinotefuran, imidacloprid) on calico scales and their natural enemies on infested thornless honeylocust trees (Gleditsia triacanthos inermis) over three years. Bifenthrin, dinotefuran and cyantraniliprole provided the largest reductions in scale populations when they were applied to egg laying females on branches early in the season. In contrast, bifenthrin and pyriproxyfen provided the most …

Fate Of 17Α-Estradiol, 17Β-Estradiol, And Estrone In Agricultural Soils And Sediments, Michael L. Mashtare Jr

Open Access Dissertations

The shift to concentrated animal production facilities and increasing rural-urban migration has increased the localized land application of nearly 1 billion tons of manure and biosolids annually. Although these applications provide nutrients and contribute to soil tilth, they also serve as a source for an estimated 49 tons of the natural manure-borne estrogens, 17α-estradiol (17α-E2), 17β-estradiol (17β-E2), and estrone (E1). While these estrogens are critical to endocrine systems, the low concentrations observed in the environment can disrupt endocrine function in non-target organisms, e.g., altering secondary sex characteristics which can lead to changes in wildlife communities.

Research presented here focuses on …

Biology Department Newsletter, No. 3, Sacred Heart University

Biology Newsletter

No abstract provided.

Sub1a-Mediated Submergence Tolerance Response In Rice Involves Differential Regulation Of The Brassinosteroid Pathway, Aaron J. Schmitz, Jing J. Folsom, Yusuke Jikamaru, Pamela C. Ronald, Harkamal Walia

Department of Agronomy and Horticulture: Faculty Publications

Submergence 1A (SUB1A), is an ethylene response factor (ERF) that confers submergence tolerance in rice (Oryza sativa) via limiting shoot elongation during the inundation period. SUB1A has been proposed to restrict shoot growth by modulating gibberellic acid (GA) signaling.

Our transcriptome analysis indicated that SUB1A differentially regulates genes associated with brassinosteroid (BR) synthesis during submergence. Consistent with the gene expression data, the SUB1A genotype had higher brassinosteroid levels after submergence compared to the intolerant genotype. Tolerance to submergence can be activated in the intolerant genotype by pretreatment with exogenous brassinolide, which results in restricted shoot elongation …

Characterisation Of Synthetically Developed Cry1ab Gene In Transgenic Tobacco Chloroplasts, Safdar Ali Mirza, Muhammad Sarwar Khan

Turkish Journal of Botany

Synthetic cry1Ab gene was developed with optimised codons and expressed in the tobacco plastome. Characterisation of the synthetic cry1Ab gene was carried out on homoplasmic transgenic shoots. To purify the homoplasmic shoots, the proliferating heteroplasmic shoots were subjected to various rounds of selection and regeneration on spectinomycin-containing regeneration medium of plant medium. The PCR-screened homoplasmic shoots were placed on MS medium for root induction and proliferation. The functionally expressed CRY protein was detected using ImmunoStrips, and the amount of toxin protein in transplastomic tobacco plants was measured by DAS-ELISA, specific for cry1Ab gene product. The accumulation of Bacillus thuringiensis (Bt) …

Three Pyronemataceous Macrofungi Genera New To Turkish Mycota, Ilgaz Akata, Abdullah Kaya

Turkish Journal of Botany

Three pyronemataceous macrofungi genera, Acervus Kanouse, Neottiella (Cooke) Sacc., and Rhodoscypha Dissing & Sivertsen, are recorded for the first time from Turkey, based on the collection of Acervus epispartius (Berk. & Broome) Pfister, Neottiella rutilans (Fr.) Dennis, and Rhodoscypha ovilla (Peck) Dissing & Sivertsen. Short descriptions of the taxa are provided together with photographs related to macro- and micromorphologies.

Asperula Anatolica (Rubiaceae), A New Species From South-East Anatolia, Turkey, Meryem Şeker

Turkish Journal of Botany

A new species, Asperula anatolica M.Ozturk (Rubiaceae), is described from south-east Turkey. A. anatolica grows on limestone bedrock, in Uludere District (C9 Şırnak Province). It is an endemic confined to south-east Anatolia, and it is related to A. comosa Schönb.-Tem in the section Cruciana. Asperula anatolica is distinct from a closely allied species, A. comosa, by its inflorescence, indumentum, corolla lobes and shape, bracts, bracteoles, internodes, seed coat surface, and pollen grains. Photos of the new species are provided, and observations on the population are discussed. Special attention is also given to its ecology and conservation status. In addition, pollen …

Physiological Responses To Nitrate Stress Of Transgenic Tobacco Plants Harbouring The Cucumber Mitogen-Activated Protein Kinase Gene, Huini Xu, Xudong Sun, Xiaoyu Yang, Qinghua Shi, Xiufeng Wang

Turkish Journal of Botany

The physiological responses to nitrate stress of 2 transgenic tobacco lines containing the cucumber mitogen-activated protein kinase (CsNMAPK) gene were investigated. Seed germination rates of the transgenic tobacco lines were higher than that of the wild type (WT) tobacco under 150 mM nitrate treatment. The transgenic seedlings had higher root fresh weight (FW) and dry weight (DW) than the WT plants after 98 mM and 182 mM nitrate treatment. The malondialdehyde (MDA) content, electrolytic leakage (EL), and H_{2}O_2 content were higher in the WT than they were in the transgenic plants after 7-day nitrate stress treatment. The antioxidant enzyme (superoxide …

The Revision Of The Genus Oxytropis (Leguminosae) In Turkey, Seher Karaman Erkul, Zeki̇ Aytaç

Turkish Journal of Botany

A taxonomic revision of the genus Oxytropis DC. (Leguminosae) in Turkey is presented. According to the recent literature, the genus had 13 taxa in Turkey; however, at the end of our studies the number was reduced to 11. It was determined that Oxytropis engizekensis Duman & Vural is a synonym of O. persica Boiss., and O. fominii Grossh. is a synonym of O. argyroleuca Bornm. Red List categories of the taxa were re-evaluated according to our results based on IUCN Red List categories. The Red List categories of O. pallasii Pers. and O. pilosa (L.) DC., which are ascribed as …

Soil-Plant Relations In The Annual Gypsophila (Caryopyhllaceae) Taxa Of Turkey, Mustafa Korkmaz, Hasan Özçeli̇k

Turkish Journal of Botany

The soil properties of the annual Gypsophila L. taxa of Turkey and their effects on plant morphology were investigated. The taxa studied were Gypsophila heteropoda Freyn & Sint., G. parva Barkoudah, G. elegans M.Bieb., G. bitlisensis Barkoudah, G. viscosa Murray, G. antari Post & Beauverd, G. muralis L., G. tubulosa (Jaub. & Spach) Boiss., G. confertifolia Hub.-Mor., and G. pilosa Hudson. The soils of the plant taxa were medium textured, sand or sandy-loam, saltless or a little salty, neutral, limy, rich in potassium and with very little phosphorous, and with medium levels of nitrogen and organic matter. G. antari, which …

Patterns Of Aquatic Macrophyte Species Composition And Distribution In Bulgarian Rivers, Gana Gecheva, Lilyana Yurukova, Svetoslav Cheshmedjiev

Turkish Journal of Botany

The composition of aquatic bryophyte and vascular plant assemblages, and 4 environmental variables (water velocity, shading, mean depth, and substrate type), together with altitude, ecological status, and river type were monitored at 223 sites along 204 rivers during 2009 in the Pontic Province and Eastern Balkan Ecoregions, Bulgaria. The relationships between macrophytes themselves and the parameters studied were tested. Forty-nine bryophytes and 86 vascular plants were identified. Canonical correspondence analysis (CCA) showed that aquatic bryophytes and vascular plants are in inverse correlation. Bryophytes occurred mainly in conditions of higher altitude and ecological status; at the same time vascular plants were …

Spatial Pattern Analysis Of Peganum Harmala On The Salted Lower Chelif Plain, Algeria, Adda Ababou, Mohammed Chouieb, Abdelkader Bouthiba, Djamel Saidi, Mahfoud M'Hamedi Bouzina, Khalladi Mederbal

Turkish Journal of Botany

Peganum harmala L. (Zygophyllaceae), native to arid and semiarid rangeland, has been widely studied biochemically, pharmaceutically, and medicinally, but is largely unknown ecologically. In the lower Chelif, Algeria, this plant is largely present and shows a clear clumped pattern, which indicates the strong effects of environmental conditions. Our redundancy analysis showed that the geographic distribution of this species was positively correlated with sand and pH and negatively correlated with conductivity, Na^+, and clay. The Gaussian model suggested that 9.4 mmhos/cm conductivity and sand contents greater than 40% are optimum for this plant. The spatial distribution map obtained through the kriging …

Physiological And Ultrastructural Studies On Calcium Oxalate Crystal Formation In Some Plants, Fayza Faheed, Ahmed Mazen, Shereen Abd Elmohsen

Turkish Journal of Botany

Results from light, scanning, and transmission electron microscopic examination of CaOx crystal formation in 2 plant species, namely Corchorus olitorius L. and Malva parviflora L., revealed the presence of crystalline deposits. These deposits were of prismatic type in the case of C. olitorius and of druse type in the case of M. parviflora. Ultrastructurally, some differences were observed between crystal idioblast and noncrystal cells. Moreover, the effect of heavy metals and different calcium concentrations on the growth of calcium oxalate crystals in leaves of the 2 plants was investigated. Different analytical techniques were used to determine the influence of exogenous …