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Micropropagación De Hedeoma Drummondii Benth, Su Lin Zamora-Hierro, Ana Laura López-Escamilla

Estudios científicos en el estado de Hidalgo y zonas aledañas

La especie Hedeoma drummondii Benth (Lamiaceae) es una planta útil de Hidalgo, México, con uso medicinal, comestible y con potencial de insecticida por el contenido de pulegona en sus aceites esenciales extraídos. En parte por su valor, la especie ha sido sobrecolectada en el medio silvestre, por lo que es necesario establecer estrategias como la micropropagación para la conservación de la misma y para la obtenció sus aceites esenciales. Este trabajo es un reporte de los avances en el cultivo de H. drummondii. Semillas de H. drummondii se desinfectaron superficialmente y se colocaron en cuatro medios de cultivo diferentes: …

Characterization And Investigation Of At2g42005 In Arabidopsis Thaliana, A Nematode-Induced Transporter, Rachel Medina

Undergraduate Review

Meloidogyne incognita is an obligate parasitic roundworm that infects the root tips of a wide variety of host plants. This infection directly affects the crop production globally with the loss of crops such as tomatoes, carrots, and potatoes. Nematode infection manipulates the plant root by altering gene expression for nematode benefit. Genes such as At2g42005, a putative amino acid transporter, have been found to be upregulated by nematode infection. Characterizing the role of the At2g42005 gene in Arabidopsis thaliana is the first step to understanding its function in nematode infection. The role of At2g42005 were explored by creation of a …

Comparative Studies Of Differential Gene Calling Using Rna-Seq Data, Ximeng Zheng, Etsuko N. Moriyama

Center for Plant Science Innovation: Faculty Publications

Background: With its massive amount of data, gene-expression profiling by RNA-Seq has many advantanges compared with microarray experiments. RNA-Seq analysis, however, is fundamentally different from microarray data analysis. Techniques developed for analyzing microarray data thus cannot be directly applicable for the digital gene expression data. Several statistical methods have been developed for identifying differentially expressed genes specifically from RNA-Seq data over the past few years.
Results: In this study, we examined the performance of differential gene-calling methods using RNA-Seq data in practical situations. We focused on two representative methods: one parametric method, DESeq, and one nonparametric method, NOISeq. …

A Novel Function Prediction Approach Using Protein Overlap Networks, Shide Liang, Dandan Zheng, Daron M. Standley, Huarong Guo, Chi Zhang

Center for Plant Science Innovation: Faculty Publications

Background: Construction of a reliable network remains the bottleneck for network-based protein function prediction. We built an artificial network model called protein overlap network (PON) for the entire genome of yeast, fly, worm, and human, respectively. Each node of the network represents a protein, and two proteins are connected if they share a domain according to InterPro database.
Results: The function of a protein can be predicted by counting the occurrence frequency of GO (gene ontology) terms associated with domains of direct neighbors. The average success rate and coverage were 34.3% and 43.9%, respectively, for the test genomes, …

A Thraustochytrid Diacylglycerol Acyltransferase 2 With Broad Substrate Specificity Strongly Increases Oleic Acid Content In Engineered Arabidopsis Thaliana Seeds, Chunyu Zhang, Umidjon Iskandarov, Elliott T. Klotz, Robyn L. Stevens, Rebecca E. Cahoon, Tara J. Nazarenus, Suzette L. Pereira, Edgar B. Cahoon

Center for Plant Science Innovation: Faculty Publications

Diacylglycerol acyltransferase (DGAT) catalyses the last step in acyl-CoA-dependent triacylglycerol (TAG) biosynthesis and is an important determinant of cellular oil content and quality. In this study, a gene, designated TaDGAT2, encoding a type 2 DGAT (DGAT2)-related enzyme was identified from the oleaginous marine protist Thraustochytrium aureum. The deduced TaDGAT2 sequence contains a ~460 amino acid domain most closely related to DGAT2s from Dictyostelium sp. (45–50% identity). Recombinant TaDGAT2 restored TAG biosynthesis to the Saccharomyces cerevisiae H1246 TAG-deficient mutant, and microsomes from the complemented mutant displayed DGAT activity with C16 and C18 saturated and unsaturated fatty acyl-CoA and diacylglycerol …

Oaks For Nebraska, Justin R. Evertson

Nebraska Statewide Arboretum: Publications

R = belongs to red oak group—acorns mature over two seasons & leaves typically have pointed lobes.
W = belongs to white oak group— acorns mature in one season & leaves typically have rounded lobes.

Estimated size range is height x spread for trees growing in eastern Nebraska.

A few places to see oaks: Indian Cave State Park; Krumme Arboretum in Falls City; Peru State College; Fontenelle Nature Center in Bellevue; Elmwood Park in Omaha; Wayne Park in Waverly; University of Nebraska Lincoln; Lincoln Regional Center Arboretum; Ehman Park in Gothenburg; Highland Park in Hastings; UNL Extension Center & Cody …

Late Gestation Supplementation Of Beef Cows Differing In Body Condition Score: Effects On Cow And Calf Performance, D. W. Bohnert, L. A. Stalker, R. R. Mills, A. Nyman, S. J. Falck, R. F. Cooke

West Central Research and Extension Center, North Platte

A 2-yr study utilizing 120 mature, crossbred (Angus × Herford) cows/year, evaluated the influence of cow BCS and dried distillers grains with solubles (DDGS) supplementation during late gestation on cow performance and productivity of subsequent offspring. Treatments were arranged as a 2 × 2 factorial in a randomized complete block design with 2 BCS and with or without DDGS supplementation. Cows were nutritionally managed to enter the last trimester of gestation with a BCS of approximately 4 (LBCS) or 6 (HBCS) and were thereafter managed in a single herd (initial BCS were 4.4 and 5.7 for LBCS and HBCS treatments, …

Identification Of Sources Of Resistance To Damping-Off And Early Root/Hypocotyl Damage From Rhizoctonia Solani In Common Bean (Phaseolus Vulgaris L.), P. A. Peña, James R, Steadman, Kent M. Eskridge, Carlos A. Urrea

Panhandle Research and Extension Center

Rhizoctonia solani causes economically important root and hypocotyl diseases in common bean throughout the world. Root health is a vital factor in plant development and root diseases would negatively influence water and nutrient uptake as well as cause direct stand reduction and root rot damage to the crop. An efficient common bean screening method to evaluate damping-off and early root/ hypocotyl damage from R. solani was developed and used to identify dry bean lines with levels of resistance to this disease. Two sets of 163 and 111 lines previously evaluated for drought tolerance in Nebraska and Puerto Rico were evaluated …

Fuzzy Clustering Of Cpp Family In Plants With Evolution And Interaction Analyses, Tao Lu, Yongchao Dou, Chi Zhang

Center for Plant Science Innovation: Faculty Publications

Background: Transcription factors have been studied intensively because they play an important role in gene expression regulation. However, the transcription factors in the CPP family (cystein-rich polycomb-like protein), compared with other transcription factor families, have not received sufficient attention, despite their wide prevalence in a broad spectrum of species, from plants to animals. The total number of known CPP transcription factors in plants is 111 from 16 plants, but only 2 of them have been studied so far, namely TSO1 and CPP1 in Arabidopsis thaliana and soybean, respectively.
Methods: In this work, to study their functions, we applied …

Acyl-Lipid Metabolism, Younghua Li-Beisson, Basil Shorrosh, Fred Beisson, Mats X. Andersson, Vincent Arondel, Philip D. Bates, Sébastien Baud, David Bird, Allan Debono, Timothy P. Durrett, Rochus B. Franke, Ian A. Graham, Kenta Katayama, Amélie A. Kelly, Tony Larson, Jonathan E. Markham, Martine Miquel, Isabel Molina, Ikuo Nishida, Owen Rowland, Lacey Samuels, Katherine M. Schmid, Hajime Wada, Ruth Welti, Changcheng Xu, Rémi Zallot, John Ohlrogge

Scholarship and Professional Work - LAS

Acyl lipids in Arabidopsis and all other plants have a myriad of diverse functions. These include providing the core diffusion barrier of the membranes that separates cells and subcellular organelles. This function alone involves more than 10 membrane lipid classes, including the phospholipids, galactolipids, and sphingolipids, and within each class the variations in acyl chain composition expand the number of structures to several hundred possible molecular species. Acyl lipids in the form of triacylglycerol account for 35% of the weight of Arabidopsis seeds and represent their major form of carbon and energy storage. A layer of cutin and cuticular waxes …

Direct And Indirect Selection On Floral Pigmentation By Pollinators And Seed Predators In A Color Polymorphic South African Shrub, Jane E. Carlson, Kent E. Holsinger

EEB Articles

The coexistence of different color morphs is often attributed to variable selection pressures across space, time, morph frequencies or selection agents, but the routes by which each morph is favored are rarely identified. In this study we untangle the interactions and trait pleiotropisms that influence floral color polymorphisms on a local scale in Protea, within which ~40% of species are polymorphic. Previous work shows that seed predators and reproductive differences likely influence polymorphism maintenance in four Protea species. Here, we explore whether selection acts on floral color directly or indirectly in Protea aurea, using path analysis of pollinator …

Pollen Morphology Of Hymenosphace And Aethiopis Sections Of The Genus Salvia (Lamiaceae) In Turkey, Hülya Özler, Sevi̇l Pehli̇van, Ferhat Celep, Musa Doğan, Ahmet Kahraman, Ahter Fi̇şne, Bi̇rol Başer, Safi Bagherpour

Turkish Journal of Botany

Palynological characteristics of 30 Salvia taxa in sections Hymenosphace and Aethiopis from Turkey were investigated by light and scanning electron microscopy. S. aethiopis (sect. Aethiopis) has the smallest pollen while S. blepharochlaena (sect. Hymenosphace) has the largest pollen. The basic shape of the pollen grains in most taxa is suboblate, oblate-spheroidal, or prolate-spheroidal to spheroidal; however, subprolate pollen grains are occasionally are found in S. cassia of sect. Aethiopis. Hexacolpate pollen is dominant in all studied taxa, but heptacolpate and octacolpate pollen grains are mixed together in S. palaestina (heptacolpate, 20%) and S. candidissima subsp. candidissima (heptacolpate, 2% and octacolpate, …

The Value And Propriety Of Reintroduction As A Conservation Tool For Rare Plants, Edward O. Guerrant Jr.

Environmental Science and Management Faculty Publications and Presentations

Three recent reviews of reintroduction for conservation purposes, which draw on substantial and largely non-overlapping data sets, have come to strikingly different conclusions about its value. One concludes that “reintroduction is generally unlikely to be a successful conservation strategy as currently conducted.” Another that “…this review cannot conclusively comment on the effectiveness of re-introductions…” The third concludes there is “strong evidence in support of the notion that reintroduction, especially in combination with ex situ conservation, is a tool that can go a long way toward meeting the needs it was intended to address.” The argument over the conservation value of …

Uses Of Phage Display In Agriculture: Sequence Analysis And Comparative Modeling Of Late Embryogenesis Abundant Client Proteins Suggest Protein-Nucleic Acid Binding Functionality, Rekha Kushwaha, A. Bruce Downie, Christina M. Payne

Horticulture Faculty Publications

A group of intrinsically disordered, hydrophilic proteins-Late Embryogenesis Abundant (LEA) proteins-has been linked to survival in plants and animals in periods of stress, putatively through safeguarding enzymatic function and prevention of aggregation in times of dehydration/heat. Yet despite decades of effort, the molecular-level mechanisms defining this protective function remain unknown. A recent effort to understand LEA functionality began with the unique application of phage display, wherein phage display and biopanning over recombinant Seed Maturation Protein homologs from Arabidopsis thaliana and Glycine max were used to retrieve client proteins at two different temperatures, with one intended to represent heat stress. From …

Bioactive Natural Products, A. Hamid A. Hadi, Mehmet Emin Duru, Ana Belen Martin-Diana

Other resources

Bioactive natural products have never ceased to play an important role in the search of novel therapeutic agents. Natural products from the nature such as the plant and marine sources form an integral part of the living system, and they have existed ever since the beginning of life. Therefore, it is undeniable that works related to natural products continue to develop in many aspects involving researchers from various scientific backgrounds. This special issue is dedicated to compile twelve articles on chemical and biological aspects of research developments in natural products.

Critical Research Needs For Successful Food Systems Adaptation To Climate Change, Michelle Miller, Molly Anderson, Charles A. Francis, Chad Kruger, Carol Barford, Jacob Park, Brent H. Mccown

Department of Agronomy and Horticulture: Faculty Publications

There is a growing sense of the fragility of agricultural production in the Global North and South and of increasing risks to food security, as scientific observations confirm significant changes in the Gulf Stream, polar ice, atmospheric CO₂, methane release, and other measures of climate change. This sense is heightened as each of us experiences extreme weather, such as the increasing frequency of droughts, floods, unseasonal temperatures, and erratic seasonality. The central research challenge before us is how global, national, regional, and local food systems may adapt to accelerating climate change stresses and uncertainties to ensure the availability, …

Arbuscular Mycorrhizal Fungi Differ In Their Ability To Regulate The Expression Of Phosphate Transporters In Maize (Zea Mays L.), Hui Tian, Rhae A. Drijber, Xiaolin Li, Daniel N. Miller, Brian J. Wienhold

Department of Agronomy and Horticulture: Faculty Publications

Previous studies have found that some phosphate (Pi) starvation inducible transporter genes are downregulated and arbuscular mycorrhizal (AM) inducible Pi transporter genes are upregulated in maize roots associated with the fungus Glomus intraradices. However, little is known about the functional diversity of different AM fungal species in influencing the expression of Pi transporters in maize roots. Here, we studied the expression of two Pi transporter genes ZEAma:Pht1;3 (Pi starvation inducible) and ZEAma:Pht1;6 (AM inducible) in maize root colonized by different AM fungal inoculants. Non-mycorrhizal maize, maize colonized by Glomus deserticola (CA113), Glomus intraradices (IA506), Glomus mosseae (CA201), Gigaspora gigantea …

Distribución De La Herpetofauna En Cuatro Tipos De Vegetación Del Estado De Hidalgo, México, Uriel Hernández-Salinas, Aurelio Ramírez-Bautista

Estudios científicos en el estado de Hidalgo y zonas aledañas

Este estudio se realizó en un periodo de un año (2007-2008), con el propósito de elaborar una lista de especies de anfibios y reptiles que habitan en cuatro tipos de vegetación: bosque mesófilo de montaña (BMM), bosque de pino-encino (BPE), matorral xerófilo (MX) y bosque tropical perennifolio (BTP) del estado de Hidalgo, y comparar la riqueza de especies entre éstos. Se encontró que el MX fue el tipo de vegetación con la menor riqueza de especies (H = 2.942), mientras que el BMM presentó la mayor de riqueza (H = 3.267). El BTP y el BPE fue la combinación vegetal …

Germinación In Vitro De Laelia Speciosa (Kunth) Schltr., Una Herramienta Para Su Conservación Ex Situ, María Ascención Aguilar-Morales, Ana Laura López-Escamilla

Estudios científicos en el estado de Hidalgo y zonas aledañas

Dentro de la familia Orchideaceae, Laelia speciosa (Kunth) Schltr. es probablemente la orquídea más explotada en México, por lo que es importante establecer estrategias de conservación. El objetivo del presente trabajo fue determinar el mejor medio de cultivo para la germinación in vitro de semillas. Cápsulas maduras e inmaduras de L. speciosa, fueron colectadas en la Reserva de la Biosfera Barranca de Metztitlán, Hidalgo, México y las semillas se sembraron en condiciones in vitro en cuatro tratamientos: Murashige y Skoog (MS) (1962) al 100%; MS al 50%; Knudson C (KC) (1946) al 50% y KC al 100%. A los …

Evaluación De Diferentes Tratamientos Pregerminativos Y Osmóticos En La Germinación De Semillas Prosopis Laevigata (Humb. & Bonpl. Ex Willd) M. C. Johnston, Jorge Alejandro Sobrevilla-Solís, Maritza López-Herrera, Ana Laura López-Escamilla, Leticia Romero-Bautista

Estudios científicos en el estado de Hidalgo y zonas aledañas

El mezquite es un elemento importante en la vegetación de ambientes semiáridos ya que tiene un gran valor ambiental. Biológicamente, las semillas de Prosopis laevigata presentan dificultades de germinación debido a la presencia de una testa impermeable causando una latencia física. El objetivo de esta investigación fue evaluar 10 diferentes tratamientos de escarificación y la combinación de los tratamientos que obtuvieron los mayores porcentajes de germinación, con cuatro soluciones osmóticas para aumentar la germinación de P. laevigata. Los índices de germinación demuestran que los tratamientos de escarificación de índole mecánico, son los de mayor efectividad ya que estos dañan …

Propagación In Vitro De Mammillaria Schiedeana Schiedeana (Cactaceae), Subespecie Endémica Y Amenazada De Extinción De La Barranca De Metztitlán, Hidalgo, Daniela Soria-Campos, Ana Laura López-Escamilla, Laura Patricia Olguín-Santos

Estudios científicos en el estado de Hidalgo y zonas aledañas

Se reporta por primera vez la micropropagación de la subespecie Mammillaria schiedeana schiedeana, cactácea mexicana considerada como amenazada de extinción por la Norma Oficial Mexicana. Se obtuvo la formación de brotes por activación areolar utilizando explantes longitudinales procedentes de brotes regenerados de un ciclo previo de cultivo y a partir de plántulas germinadas in vitro los cuales se sembraron en medio basal Murashige y Skoog (MS) suplementado con 6-bencilaminopurina (BA) y ácido α-naftalenacético (ANA) a diferentes concentraciones. La producción de brotes en el medio de proliferación se evaluó después de dos meses. La mejor concentración para la formación de …

Plantas Medicinales De Omitlán, Hidalgo, México, I. Fuentes-Cervantes, Miguel Ángel Villavicencio-Nieto, Blanca Estela Pérez-Escandón

Estudios científicos en el estado de Hidalgo y zonas aledañas

Se seleccionó la cabecera municipal de Omitlán, Hidalgo, con el objetivo de evaluar el uso de plantas medicinales, documentar la importancia de dichas especies y estimar el estado actual del conocimiento tradicional en la comunidad. Por medio de entrevistas se detectaron las especies de plantas de uso medicinal, las cuales fueron recolectadas e identificadas, y se llevó a cabo el análisis de datos. Se registraron 134 etnoespecies de plantas, se identificaron 102 especies, las cuales están distribuidas en 53 familias y 89 géneros. La familia y género mejor representados son Asteraceae y Sedum. En el promedio de menciones por …

Transcriptome Characterization And Detection Of Gene Expression Differences In Aspen (Populus Tremuloides), Hardeep S. Rai, Karen E. Mock, Bryce A. Richardson, Richard C. Cronn, Katherine J. Hayden, Jessica W. Wright, Brian J. Knaus, Paul G. Wolf

Aspen Bibliography

No abstract provided.

Wolf Creek Ranch Aspen Monitoring Report, Paul C. Rogers, Allison Jones, James Catlin, James Shuler, Arthur Morris, Michael Kuhns

Aspen Bibliography

In the summer of 2012 we undertook a landscape assessment of aspen forest conditions at Wolf Creek Ranch (WCR) near Kamas, Utah. Using a geographic information system (GIS) coverage of predicted aspen coverage, fifty systematically selected field "plots" were located and mapped with aspen stands around WCR. Seven plots were eventually discarded from our survey due to lack of aspen cover. Volunteer field crews, mostly WCR homeowners, collected aspen data documenting tree sizes, height/age groups, diameters, reproduction, mortality, browse levels, and browse animal use levels.

Differential Allocation To Photosynthetic And Non-Photosynthetic Nitrogen Fractions Among Native And Invasive Species, Jennifer L. Funk, Lori A. Glenwinkle, Lawren Sack

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Invasive species are expected to cluster on the “high-return” end of the leaf economic spectrum, displaying leaf traits consistent with higher carbon assimilation relative to native species. Intra-leaf nitrogen (N) allocation should support these physiological differences; however, N biochemistry has not been examined in more than a few invasive species. We measured 34 leaf traits including seven leaf N pools for five native and five invasive species from Hawaii under low irradiance to mimic the forest understory environment. We found several trait differences between native and invasive species. In particular, invasive species showed preferential N allocation to metabolism (amino acids) …

Synthesis, Characterization, And Properties Of Mononuclear And Dinuclear Ruthenium(Ii) Complexes Containing Phenanthroline And Chlorophenanthroline, Anwar A. Bhuiyan, X. Du

Journal of the Arkansas Academy of Science

The study of photophysical and photochemical properties of ruthenium complexes is of great interest for fundamental practical reasons. Ruthenium complexes have been investigated for use in artificial photosynthesis. This paper deals with the synthesis and spectroscopic investigation of custom-designed ruthenium complexes containing phenanthroline and chloro-phenanthroline ligands. These complexes maybe useful for biological electron-transfer studies. The heteroleptic ruthenium monomer complex Ru(phen)2(Cl-phen) (where phen = 1,10-phenanthroline and Cl-phen=5-chloro-1,10-phenanthroline) was prepared in a two-step procedure previously developed in our laboratory. This monomer complex was used to prepare the ruthenium homometallic dimer complex, (phen)2Ru(phen-phen)Ru(phen)2, by utilizing the Ni-catalyzed coupling reaction. Both complexes were purified …

Impacts Of Spring-Interseeded Cover Crops On Late-Emerging Weed Suppression And Ground Cover In Corn (Zea Mays L.) Production Systems, Alex D. Bich

Electronic Theses and Dissertations

Any alternative crop management strategy will only be adopted by growers if yield is not negatively impacted, fits within a current management practice, is easily implementable with minimal cost, or provides other beneficial features such as weed control or suppression. Corn production that incorporates a cover crop as an alternative weed control strategy and ground cover may support a sustainable system that is less dependent on herbicidal weed control. In addition, the cover crop may be used as a fall forage, act as a slow release fertilizer source the following year, and provide ground cover to reduce soil erosion. In …

Efficacy Of Foliar Fungicides And Application Timing Of Stratego Yld On Field Corn In Nebraska, 2013, C. M. Schleicher, T. A. Jackson-Ziems

Department of Plant Pathology: Faculty Publications

A foliar fungicide efficacy timing trial was conducted at the University of Nebraska-Lincoln South Central Agricultural Laboratory near Clay Center, NE. Corn hybrid NK ‘N68B’, rating of “fair” (7 out of 9) for gray leaf spot (GLS), not rated for common rust (CR), and “excellent” (2 out of 9) for southern rust (SR), was planted on 15 May in 30-in. rows at a target population of 31,763 plants/A. The trial area was disked with corn as the previous year’s crop. Nine treatments and a nontreated control were replicated six times in a randomized complete block design. Each plot was four …

Evaluation Of Foliar Fungicide And Application Timing On Field Corn Following Hail Event At Scal In Nebraska, 2013, C. M. Schleicher, T. A. Jackson-Ziems

Department of Plant Pathology: Faculty Publications

A foliar fungicide efficacy timing trial was conducted at the University of Nebraska-Lincoln South Central Agricultural Laboratory near Clay Center, NE to determine the potential effect of foliar fungicides and their application timing on lodging and yield after a late season hail event. Novartis corn hybrid N6800 GT with CruiserMaxx 250 seed treatment was planted on 24 May in 30 in. rows with a target population of 32,000 plants/A. Force 3G (4 oz/1,000 row ft) was applied in-furrow at planting. Lexar (3 qt/A) + Roundup UltraMax (32 fl oz/A) was applied on 5 Jun. The trial area had corn as …

Efficacy Evaluation Of Fortix And Headline Amp Application Timing In Field Corn In Nebraska, 2013, C. M. Schleicher, T. A. Jackson-Ziems

Department of Plant Pathology: Faculty Publications

A foliar fungicide efficacy timing trial was conducted at the University of Nebraska-Lincoln South Central Agricultural Laboratory near Clay Center, NE. Corn hybrid NK ‘N68B’, rating of “fair” (7 out of 9) for gray leaf spot (GLS), not rated for common rust (CR), and “excellent” (2 out of 9) for southern rust (SR), was planted on 15 May in 30-in. rows at a target population of 31,763 plants/A. The trial area was disked with corn as the previous year’s crop. Nine treatments were replicated six times in a randomized complete block design. Each plot was four rows (10 ft) wide …