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Electrogenic Uptake Of Gamma-Aminobutyric Acid By A Cloned Transporter Expressed In Xenopus Oocytes, Michael Kavanaugh, Jeffrey L. Arriza, R. Alan North, Susan G. Amara

Biomedical and Pharmaceutical Sciences Faculty Publications

GAT-1, a gamma-aminobutyric acid (GABA) transporter cloned from rat brain, was expressed in Xenopus oocytes. Voltage-clamp measurements showed concentration-dependent, inward currents in response to GABA (K0.5 4.7 microM). The transport current required extracellular sodium and chloride ions; the Hill coefficient for chloride was 0.7, and that for sodium was 1.7. Correlation of current and [3H]GABA uptake measurements indicate that flux of one positive charge occurs per molecule of GABA transported. Membrane hyperpolarization from -40 to -100 mV increased the transport current approximately 3-fold. The results indicate that the transport of one molecule of GABA involves the co-transport of two sodium …

Cooperative Interactions Among Subunits Of A Voltage-Dependent Potassium Channel. Evidence From Expression Of Concatenated Cdnas, Raymond S. Hurst, Michael Kavanaugh, Jerrel Yakel, John P. Adelman, R. Alan North

Biomedical and Pharmaceutical Sciences Faculty Publications

Four copies of the coding sequence for a voltage-dependent potassium channel (RBK1, rat Kv1.1) were ligated contiguously and transcribed in vitro. The resulting RNA encodes four covalently linked subunit domains ([4]RBK1). Injection of this RNA into Xenopus oocytes resulted in the expression of voltage-dependent potassium currents. A single amino acid substitution, Tyr-->Val, located within the outer mouth of the pore, introduced into the equivalent position of any of the four domains, reduced affinity for external tetraethylammonium by approximately the same amount. In constructs containing 0, 1, 2, 3, or 4 Tyr residues the free energy of binding tetraethylammonium was …

Effects Of Ecotropic Murine Retroviruses On The Dual-Function Cell Surface Receptor/Basic Amino Acid Transporter, Hao Wang, Esther Dechant, Michael Kavanaugh, R. Alan North, David Kabat

Biomedical and Pharmaceutical Sciences Faculty Publications

The widely expressed Na(+)-independent transporter for basic amino acids (system y+) is the cell surface receptor (ecoR) for ecotropic host-range mouse retroviruses (murine leukemia viruses (MuLVs)), a class of retroviruses that naturally infects only mice or rats. Accordingly, expression of mouse ecoR cDNA in mink CCL64 fibroblasts yields cells (CEN cells) that have y+ transporter activity above the endogenous background and that bind and are infected by ecotropic MuLVs. The effect of ecotropic MuLV infection on expression of y+ transporter was analyzed in mouse and in mink CEN fibroblasts. Chronic infection with ecotropic MuLVs caused 50-70% loss (down-modulation) of mouse …

University Of Texas At Tyler Catalog, 1992-1994, University Of Texas At Tyler

Catalogs

Archives Space instance: https://archivesspace.uttyler.edu/repositories/2/archival_objects/22366

Alcoholic Women's Relationships As Related To Intimacy And Trust, Cara Leona Forth

Theses Digitization Project

Community college women -- Variable : parental drinking -- The Personal Assessment of Intimacy in Relationship Inventory -- The Trust Scale.

The Effects Of Taste Quality And Spatial Location On The Potentiation Of An Aversion To A Non-Gustatory Stimulus, David Keith Kennedy

Theses Digitization Project

Taste potentiation--Spatial and temporal contiguity--No-choice suppression of ingestion--Palatable and unpalatable tastes--Distal auditory stimulus--Variant distal locations.

Outlook Magazine, Fall 1992

Outlook Magazine

No abstract provided.

Outlook Magazine, Summer 1992

Outlook Magazine

No abstract provided.

The Creation Of Transgenic Animal “Models” For Human Genetic Disease, Bernard Rollin

Biomedicine and Animal Models in Research Collection

Transgenic animals will be created to study human genetic disease as soon as the technological capability exists to do so. Extant laws permit such animals to be created. The mindset of the research community makes it inevitable. It is also clear that such diseases can cause enormous amounts of pain and suffering. Responsible researchers need to explore all possible avenues for controlling such pain and suffering. Thus far the research community has not engaged this issue vis a vis animals. The development of methodologies for controlling pain and suffering is likely to be exportable to numerous areas of animal research, …

Reproductive Characteristics Of Rodent Assemblages In Cultivated Regions Of Central Argentina, James N. Mills, Barbara A. Ellis, Kelly T. Mckee, Julio I. Maiztegui, James E. Childs

Zoonotics and Wildlife Disease: Publications

Small mammals were trapped for 2 years at 16 localities on the central-Argentine pampa. Six species (Akodon azarae, Calomys musculinus, C. laucha, Bolomys obscurus, Oligory-zomys flavescens and Mus musculus) accounted for >95% of captures. The major breeding season, as assessed by pregnancies, was September or October through April or May. Mild weather in late autumn and winter of the second season resulted in a relatively longer breeding season during the 2nd year of the study. Females of all six species comprised significantly 50% of captures during the height of the breeding season. For most species, there was …

Outlook Magazine, Spring 1992

Outlook Magazine

No abstract provided.

Outlook Magazine, Winter 1992

Outlook Magazine

No abstract provided.

Revival Of Metabolism In Rehydrated Marasmius Oreades, Lorie J. Warren

Honors Theses, 1963-2015

Mushrooms from the genus Marasmius have the ability to revive -- viz., dried specimens regain their original shape when moistened. The purpose of this study was to determine if the basidiocarps are metabolically active upon revival or whether they simply absorb moisture like a sponge. The reduction of tetrazolium chloride to a red, water-insoluble formazan pigment was used as a measure of respiratory activity. Basidiocarp samples were incubated in a phosphate buffer containing 1% tetrasolium chloride. After a suitable period, the samples were extracted with MeOH to remove any formazan pigment and the absorbance measured at 530 nm. Fresh M. …

The Present And Future Of Prevention: In Honor Of George W. Albee, Marc Kessler, Stephen E. Goldston, Justin M. Joffe

Mental Health Law & Policy Faculty Publications

No abstract provided.

Murder, She Wrote Or Was It Merely Selective Nontreatment?, George P. Smith Ii

Scholarly Articles

This article will both explore and thereby establish the medical, ethical, and legal validity of selective nontreatment of severely handicapped newborns. A construct for principled decision-making, tied to a basic recognition of the right of self-determination, as shaped by compassion and validated principles of triage and cost-benefit analysis, will be seen as the most effective means for the states-and not the federal government-to evaluate the intensely complex issues associated with allocating scarce medical resources to defective infants. Governmental intrusions into the familial decision- making forum in these circumstances must be kept to a minimum and allowed only in grave cases.

List Of Museum Type Repositories, Stephen L. Wood, Donald E. Bright Jr.

Great Basin Naturalist Memoirs

No abstract provided.

Efficacies Of Liposome-Encapsulated Streptomycin And Ciprofloxacin Against Mycobacterium Avium-M. Intracellulare Complex Infections In Human Peripheral Blood Monocyte/Macrophages, S. Majumdar, Diana L. Flasher, Daniel S. Friend, P. Nassos, D. Yajko, W. K. Hadley, Nejat Düzgüneş

All Dugoni School of Dentistry Faculty Articles

Current treatments of disseminated infection caused by the Mycobacterium avium-M. intracellulare complex (MAC) are generally ineffective. Liposome- mediated delivery of antibiotics to MAC-infected tissues in vivo can enhance the efficacy of the drugs (N. Duzgunes, V. K. Perumal, L. Kesavalu, J. A. Goldstein, R. J. Debs, and P. R. J. Gangadharam, Antimicrob. Agents Chemother. 32:1404-1411, 1988; N. Duzgunes, D. A. Ashtekar, D. L. Flasher, N. Ghori, R. J. Debs, D. S. Friend, and P. R. J. Gangadharam, J. Infect. Dis. 164:143-151, 1991). We investigated the therapeutic efficacies of liposome- encapsulated streptomycin and ciprofloxacin against growth of the MAC inside human …

Ms211: Background Examples Of Literature Searches On Topics Of Interest, Armin Weinberg

Works on Radiation Effects: 1990-2020

A zip file of various literature searches & some resources related to our work related to exposure after the Chernobyl accident and as we began looking at helping in Semey Kazakhstan----a collection of literature reviews on various topics we were interested in... eg. establishing a registry of those exposed for longterm follow-up, what we knew about certain areas like genetics and some resources like "A Guide to Environmental Resources" on the Internet by Carol Briggs-Erickson and Toni Murphy which could be found on the Internet and was written to be used by researchers, environmentalists, teachers and any person who is …

Phosphorylation Of The Rex Protein Of Human T-Cell Leukemia Virus Type 1, Yoshifumi Adachi, Terry D. Copeland, Chiaki Takahashi, Tetsuya Nosaka, Aftab Ahmed, Stephen Oroszlan, Masakazu Hatanaka

Pharmacy Faculty Articles and Research

Rex protein, the posttranscriptional regulator of human T-cell leukemia virus type I (HTLV-I), is required for the control of viral structural protein expression and virus replication. Rex is a phosphoprotein found predominantly in the cell nucleolus, whose function is thought to be regulated by its nucleolar localization and phosphorylation. Therefore, we investigated the in vivo phosphorylation of Rex protein in more detail. Phosphorylation of Rex occurred in all HTLV-I-infected cell lines examined in vivo, primarily at serine residues and to a very small extent at threonine residues. Treatment of cells with 12-O-tetradecanoylphorbol-13-acetate (TPA) led to significant but transient enhancement of …

A Study To Determine The Factors Associated With Generational Welfare And Long Term Welfare Dependency, Kennette A. Hughes

Retrospective Theses and Dissertations

No abstract provided.

Sequence Specificity Of Teniposide-Induced Deletion And Insertion Mutations At The Aprt Locus Of Chinese Hamster Cells, Yi-Hong Han

Theses and Dissertations

Previous studies suggested that teniposide is a strong clastogen, and that the DNA breakage effect of this drug is mediated by the nuclear enzyme topoisomerase II. Ripley et al found evidence for a correspondence between sites of acridine-induced frameshift mutations in bacteriophage T4 and sites of in Vitro DNA cleavage by T4 topoisomerase II. To identify the sequence specificity of teniposide-induced deletion and insertion mutations in mammalian cells, the CHO-D422 cell line, which is hemizygous at the aprt locus, was employed in this study. Sixty-eight teniposide-induced and 42 spontaneous aprt mutants were analyzed at the DNA sequence level. Compared with …

The Effect Of Body Position Changes On Arterial Oxygen Saturation In Obesity As Measured Noninvasively By Pulse Oximetry, Anna Marie Angela Klosterman

Theses and Dissertations

This study utilized a quasi-experimental pretest-posttest design that compared the effect of body position changes on arterial oxygen saturation in obesity as measured noninvasively by pulse oximetry. The null hypothesis, that there would be no difference between oxygen saturation values measured by pulse oximetry with position change from sitting to supine in obese subjects as compared to nonobese subjects, was tested. Fourteen obese, American Society of Anesthesiologist (ASA) physical class status II subjects and 14 nonobese ASA physical class status I subjects were included in the sample population. Thirteen of the obese subjects were further classified as morbidly obese. After …

Key To Subject Areas, Stephen L. Wood, Donald E. Bright Jr.

Great Basin Naturalist Memoirs

No abstract provided.

An Analysis Of The Values Influencing Neonatal Nurses' Perceptions And Behaviors In Selected Ethical Dilemmas, Deborah A. Raines

Theses and Dissertations

The purpose of this research was to identify the values influencing the nurse's perception and choice of behavior in a hypothetical clinical situation. The theoretical framework was Rokeach's (1973) Theory on the Nature of Human Values and Value Systems. A descriptive study using a mailed survey was conducted on a random sample of 331 members of the National Association of Neonatal Nurses. Data on individual nurses' values, perception of information and behavioral choices were collected with an investigator developed questionnaire, consisting of a values scale (alpha =.82) and an information scale and choice alternatives related to three hypothetical vignettes: a …

Synapsis: Philadelphia Campus (1992), Philadelphia College Of Osteopathic Medicine

All Yearbooks

This yearbook includes Phi Sigma Gamma, PSG, (ΦΣΓ), Dinner Dance, Founders' Day Follies, Follies, Halloween, White Water Rafting, Wheal of Function, Show, Rugby, Eastern Pennsylvania Rugby Union Professional School Division Champions, Soccer, Basketball, Golf, Rowing, Yearbook Staff, Student Council, Class Officers

Bibliography, George M. Austin (1992), George M. Austin

The Dr. George M. Austin Neurology Research Collection

Bibliography, 169 entries 14 pg., last entry is for 1992

Feelings And Concerns Of New Fathers When Their Spouses Have Epidural Anesthesia During Childbirth, Susan M. Monger

Honors Projects

Over the past years, the use of epidural anesthesia as an intervention for pain relief has increased in the labor and delivery setting. Exploration of the literature has not identified what effects the addition of this technology has had on the normal childbirth process and those participating in childbirth, specifically the new father. The purpose of this study was to explore first-time fathers' perceptions of their feelings and concerns during the various phases of the labor and delivery experience in which epidural anesthesia is used. The study addresses the following research questions: (a) What are the perceptions of first-time fathers …

Oncolog, Volume 37, Issue 03, July-September 1992, Scott M. Lippman Md, Mark A. Schusterman M.D., Eva Singletary Md, Merrick I. Ross M.D., Daryl Ogden Ms

OncoLog MD Anderson's Report to Physicians (All issues) Archives

• Retinoic acid/interferon combination shows promising response rates
• Cross-matching test helps find donors for patients in dire need of platelets
• Silicone gel-filled implants: Women should have the option to choose

Oncolog, Volume 37, Issue 02, April-June 1992, Ralph S. Freedman Md, Phd, Bartlett D. Moore Iii,Phd, Nicholas H.A. Terry Phd

OncoLog MD Anderson's Report to Physicians (All issues) Archives

• The primacy of patient welfare
• Potential doubling time of tumors may be the key to accurate prognosis, appropriate treatment
• Cognitive deficits in survivors of childhood cancers

Oncolog, Volume 37, Issue 01, January-March 1992, Roman Perez-Soler Md, Waldemar Priebe Md, Michael Needle, Sidney Wallace Md

OncoLog MD Anderson's Report to Physicians (All issues) Archives

• Clinician and chemist forge new approach to liposome research
• Computerized network enhances interdisdplinary patient management
• Neurofibromatosis: a new clinic brings new opportunities