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Randomly Amplified Polymorphic Dna Analysis (Rapd) Of Artemisia Subgenus Tridentatae Species And Hybrids, E. Durant Mcarthur, Joann Mudge, Renée Van Buren, W. Ralph Andersen, Stewart C. Sanderson, David G. Babbel

Great Basin Naturalist

Species of Artemisia (subgenus Tridentatae) dominate much of western North America. The genetic variation that allows this broad ecological adaptation is facilitated by hybridization and polyploidization. Three separate studies were performed in this group using randomly amplified polymorphic DNA (RAPD). Fifty-seven 10-mer primers generated nearly 400 markers from genomic DNA obtained from leaf tissue. These studies were (1) a measure of the variability of plants within and between populations and between subspecies using 5 A. tridentata ssp. wyomingensis populations, 2 A. cana ssp. cana populations, and 1 A. cana ssp. viscidula population; (2) an examination of the hypothesis that …

A Vacuolar V–T-Snare Complex, The Predominant Form In Vivo And On Isolated Vacuoles, Is Disassembled And Activated For Docking And Fusion, Christian Ungermann, Benjamin J. Nichols, Hugh R. B. Pelham, William Wickner

Dartmouth Scholarship

Homotypic vacuole fusion in yeast requires Sec18p (N-ethylmaleimide-sensitive fusion protein [NSF]), Sec17p (soluble NSF attachment protein [alpha-SNAP]), and typical vesicle (v) and target membrane (t) SNAP receptors (SNAREs). We now report that vacuolar v- and t-SNAREs are mainly found with Sec17p as v-t-SNARE complexes in vivo and on purified vacuoles rather than only transiently forming such complexes during docking, and disrupting them upon fusion. In the priming reaction, Sec18p and ATP dissociate this v-t-SNARE complex, accompanied by the release of Sec17p. SNARE complex structure governs each functional aspect of priming, as the v-SNARE regulates the rate of Sec17p release and, …

Oppositional Defiant Disorder And Conduct Disorder : Different Names For The Same Condition?, Daryl L. Hawkins

Graduate Research Papers

The purpose of this paper is to present a review of the literature related to the proposition that oppositional defiant disorder and conduct disorder are, in essence, the same disorder, with varying degrees of severity. The author of this paper believes that it would make more sense diagnostically to combine these two diagnoses into one category.

This paper will cover a variety of areas related to oppositional defiant disorder and conduct disorder. First, a section on the formulation of the diagnostic criteria is presented. Brief definitions and descriptions of the two disorders follow the diagnostic criteria section. In addition to …

Differences In Lean And Obese Skeletal Muscle Myoblasts Of Adult And Post-Natal La/N (Faf) Rats: A Confocal Description Of The Skeletal Muscle Myoblast, J. Adam Noah

Theses, Dissertations and Capstones

Mutant strains of obese rats are currently being studied to reveal the mechanisms of increased lipid synthesis and decreased lipid oxidation in these rats. It was originally thought that much of the fat production that leads to obesity is the result of metabolic processes in the liver and adipose tissue. Studies by Kahle et al.³ showed that the liver and adipose tissue account for only 22% and 7% respectively, of total fatty acid synthesis. It was also shown that 21 % of total de novo fatty acid was localized in the postural skeletal muscle.

The dry weight of the …

Land Use Patterns In Relation To Lake Water Quality In The Great Pond Watershed, Problems In Environmental Science Course (Biology 493), Colby College, Colby Environmental Assessment Team, Colby College

Colby College Watershed Study: Great Pond (2012, 2010, 1998)

Lakes are natural resources, which have many effects on the land surrounding them, They support adjacent communities by providing water and regulating temperatures, helping to define the surrounding ecosystem, and serving as sources of drinking water as well as recreation. The prolonged presence of human activity in a watershed can disturb the physical and chemical cycles of the lake and its surrounding ecosystems (Henderson-Sellers and Markland 1987). Over time, lakes undergo a process called eutrophication, a natural aging process during which the nutrient levels increase and dissolved oxygen levels decrease (Smith and Smith 1998). As the lake ages or becomes …

Progestin Stimulation Of The Protooncogene C-̲M̲Y̲C In T47d Human Breast Cancer Cells, Kristy Ann Blankenship

Theses, Dissertations and Capstones

Moore and co-workers have previously demonstrated that progestins stimulate the growth of T47D human breast cancer cells. We now investigate the possibility that progestins might transactivate the protooncogcne c-myc as part of the mechanism for growth stimulation. Treatment of T47D cells with the synthetic progestin R5020 results in a rapid, dose-dependent increase in c-myc mRNA. This stimulation is evident as early as 5 minutes, increases up to 4-fold at 1 hour, and then returns toward basal levels. The optimal concentration of R5020 for induction of c-myc gene expression is 10 nM, which is within the physiologically relevant range of hormone …

Characterization Of The Promoter And Upstream Activation Sequences Of Sporulation-Specific Genes Spr1 (Ssg1) And Spr2 In Saccharomyces Cerevisiae, Sharmini S. Soosaithasan

Theses, Dissertations and Capstones

Sporulation is a developmental process in which temporally distinct sets of genes regulate the processes of meiosis and ascospore formation. Sporulation-specific genes are classified into early, middle, and late genes based on their peak time of expression during sporulation. Regulation of two late genes, SPR1 and SPR2, was studied. The function of SPR2 is unknown. Its regulatory region contains two Mid Sporulation Elements (MSE) repeated in tandem that are sufficient to confer sporulation-specific regulation to a CYCl-lacZ reporter. These sequences have UAS (upstream activation sequence) properties, as each 14 base pair element is sufficient to confer sporulation-specific regulation …

Managing The Iatrogenic Risks Of Risk Management, Jonathan Baert Wiener

RISK: Health, Safety & Environment (1990-2002)

Analogizing to concerns that led the practice of medicine to shift from a specialist to a team-based approach, Dr. Wiener suggests that public and environmental health objectives would be better served if, e.g., regulatory jurisdiction were less atomized.

College Of Dental Medicine-For The 1998 Entering Class, Nova Southeastern University

Health Professions Divisions Course Catalogs and Course Descriptions

No abstract provided.

Neurotransmitter Transport: Models In Flux, Michael Kavanaugh

Biomedical and Pharmaceutical Sciences Faculty Publications

Physiologists say that ions and neutral solutes can cross biological membranes via “transporters” and “channels.” We tend to think about the difference between transporters and channels in terms of gating mechanisms. Ion channels exhibit a wide range of selectivity properties and permeation rates, but their gating at the most basic level can be thought of in terms of a single barrier or gate acting as a switch. When the gate is closed, ions can’t permeate; when the gate is opened, a permeation pathway for ions allows flux, often at very high rates (up to 10⁸/sec). Transporters and ion …

Excitatory Amino Acid Transporters Of The Salamander Retina: Identification, Localization, And Function, Scott Eliasof, Jeffrey L. Arriza, Barbara H. Leighton, Michael Kavanaugh, Susan G. Amara

Biomedical and Pharmaceutical Sciences Faculty Publications

The rapid re-uptake of extracellular glutamate mediated by a family of high-affinity glutamate transporter proteins is essential to continued glutamatergic signaling and neuronal viability, but the contributions of individual transporter subtypes toward cellular physiology are poorly understood. Because the physiology of glutamate transport in the salamander retina has been well described, we have examined the expression and function of glutamate transporter subtypes in this preparation. cDNAs encoding five distinct salamander excitatory amino acid transporter (sEAAT) subtypes were isolated, and their molecular properties and distributions of expression were compared. We report evidence that at least four distinct sEAAT subtypes are expressed …

Cysteine Scanning Of The Surroundings Of An Alkali-Ion Binding Site Of The Glutamate Transporter Glt-1 Reveals A Conformationally Sensitive Residue, Ruth Zarbiv, Myriam Grunewald, Michael Kavanaugh, Baruch I. Kanner

Biomedical and Pharmaceutical Sciences Faculty Publications

Glutamate transporters remove this transmitter from the extracellular space by cotransport with three sodium ions and a proton. The cycle is completed by translocation of a potassium ion in the opposite direction. Recently we have identified two adjacent amino acid residues of the glutamate transporter GLT-1 that influence potassium coupling. Using the scanning cysteine accessibility method we have now explored the highly conserved region surrounding them. Replacement of each of the five consecutive residues 396–400 by cysteine abolished transport activity but at several other positions the substitution is tolerated. One residue, tyrosine 403, was identified where cysteine substitution renders the …

Gp120 Envelope Glycoproteins Of Human Immunodeficiency Viruses Competitively Antagonize Signaling By Coreceptors Cxcr4 And Ccr5, Navid Madani, Susan L. Kozak, Michael Kavanaugh, David Kabat

Biomedical and Pharmaceutical Sciences Faculty Publications

Signal transductions by the dual-function CXCR4 and CCR5 chemokine receptors/HIV type 1 (HIV-1) coreceptors were electrophysiologically monitored in Xenopus laevis oocytes that also coexpressed the viral receptor CD4 and a G protein-coupled inward-rectifying K⁺ channel (Kir 3.1). Large Kir 3.1-dependent currents generated in response to the corresponding chemokines (SDF-1α for CXCR4 and MIP-1α; MIP-1β and RANTES for CCR5) were blocked by pertussis toxin, suggesting involvement of inhibitory guanine nucleotide-binding proteins. Prolonged exposures to chemokines caused substantial but incomplete desensitization of responses with time constants of 5–7 min and recovery time constants of 12–19 min. CXCR4 and CCR5 exhibited heterologous …

Arachidonic Acid Activates A Proton Current In The Rat Glutamate Transporter Eaat4, Anastassios V. Tzingounis, Chien-Liang Lin, Jeffrey D. Rothstein, Michael Kavanaugh

Biomedical and Pharmaceutical Sciences Faculty Publications

The excitatory amino acid transporter EAAT4 is expressed predominantly in Purkinje neurons in the rat cerebellum (1-3), and it participates in postsynaptic reuptake of glutamate released at the climbing fiber synapse (4). Transporter-mediated currents in Purkinje neurons are increased more than 3-fold by arachidonic acid, a second messenger that is liberated following depolarization-induced Ca²⁺ activation of phospholipase A₂ (5). In this study we demonstrate that application of arachidonic acid to oocytes expressing rat EAAT4 increased glutamate-induced currents to a similar extent. However, arachidonic acid did not cause an increase in the rate …

Occupational Therapy Program 1998, Nova Southeastern University

Health Professions Divisions Course Catalogs and Course Descriptions

No abstract provided.

Postoperative Infection Rates With Instrumented Lumbar Fusion: A Retrospective Review Of 129 Consecutive Cases, Nicole G. Kurzynske, Scott D. Hodges, Stephen C. Humphreys, Jason C. Eck, Laurie C. Covington

Capstone Research Projects

Introduction: The use of internal fixation devices in spinal surgery is common to achieve reduction, maintain alignment, and stabilize the spine while fusion occurs. Infection rates of less than 2% (0-2%) were reported in the late 1960's without the use of instrumentation. Reported rates of infection following instrumented fusion are generally around 6% (range 0-11% ). The purpose of this study was to retrospectively review the incidence of postoperative infection following instrumented spinal fusion to determine if infection rates related to patient type and surgical procedure.

Methods: A retrospective review was conducted of 129 consecutive cases of instrumented lumbar spinal …

College Of Optometry-For The 1998 Entering Class, Nova Southeastern University

Health Professions Divisions Course Catalogs and Course Descriptions

No abstract provided.

Physical Therapy Program 1998, Nova Southeastern University

Health Professions Divisions Course Catalogs and Course Descriptions

No abstract provided.

College Of Osteopathic Medicine-For The 1998 Entering Class, Nova Southeastern University

Health Professions Divisions Course Catalogs and Course Descriptions

No abstract provided.

Patent: Male Contraceptive Implant, Alfred J. Moo-Young, Saleh I. Saleh

Formulation/Product Development

The present invention relates to implantable male contraceptive devices. An ethylene vinyl acetate copolymer based implant is described for delivery of an androgen and a system including an ethylene vinyl acetate copolymer based implant as well as a second implantare described for the administration of both androgen and a sterilitant. These implants may be used to provide contraception for men, as well as, for hormone therapy, treatment of enlarged prostate and other aliments.

Tumor Necrosis Factor-\Alpha And Interleukin-6 Inperitoneal Adhesion Formation, Mahmut Başoğlu, Ahmet Kiziltunç, Fatih Akçay, Sait Keleş, Cemal Gündoğdu, Durkaya Ören

Turkish Journal of Medical Sciences

This study was carried out to assess the role of tumor necrosis factor (TNF-\alpha) and interleukin-6 (IL-6 ) in plasma and peritoneal fluid in higher rates of adhesion formation following standard bowel injury. Forty-five albino rabbits were divided into three equal groups. Blood was obtained from all the rabbits preoperatively. All the rabbits were subjected to a laparotomy. In group 1(control group), the peritoneal cavity was irrigated with normal saline. In group 2 the cecal serosa was abraded, while the rabbits in group 3 had a resection in small bowel of 2 cm. A peritoneal catheter was placed in all …

Glycoprotein Iib/Iiia Inhibitors In Acute Coronarysyndromes: A New Paradigm In Cardiology, Mehmet Ağirbaşli, David M. Kerins

Turkish Journal of Medical Sciences

No abstract provided.

Characteristics And Treatment Of Conduct Disorders, Vicky Jo Kukral

Graduate Research Papers

Conduct Disorder is the most diagnosed condition in both outpatient and inpatient mental health facilities for children in the United States. Behavior problems such as aggression, destruction of property, theft and violation of basic rules are concerns for parents, teachers, peers and other adults. In an effort to help increase the reader's understanding of the Conduct Disorder population and their treatment, the author of this paper will describe and characteristics of this population. The author will also address specific mental health concerns as found in a review of the literature, and the status of mental health treatment.

Adolescent Depression : A School Perspective, Kayann Lilja

Graduate Research Papers

The path from adolescence to adulthood is ever-changing and often extremely challenging. There is growing consensus that today it is an even more difficult journey than ever before. The purpose of this paper is to synthesize current knowledge regarding adolescent depression and its relationship to normal adolescent development. In addition, the origin, nature and characteristics of adolescent depression will be discussed. The paper will conclude with suggested interventions for educators to address the issue of adolescent depression in a preventative as well as treatment manner.

Hamstring Tendons As Autograft Replacements In Acl Reconstructions: A Review Of The Literature, Jason Anderson

Physical Therapy Scholarly Projects

Hamstring tendons have gained a great deal of acceptance in anterior cruciate ligament (ACL) reconstruction procedures in the 1990's, but some misconceptions concerning the ability of hamstring grafts to successfully replace ruptured ACLs still exist. More surgeons are using hamstring tendons to reconstruct ACL-deficient knees because their harvest does not disrupt the extensor mechanism of the knee. Enthusiasts of the hamstring graft believe that it promotes quicker return of quadriceps strength and knee range of motion without a prevalence of postoperative pateIIofemoral symptoms.

The purpose of this review is to dispute the concerns that exist concerning the initial tensile strength, …

Identifying Community Specific Barriers To Prenatal Care Services, Linda Pearl Helsper

Theses Digitization Project

The intent of this research project was to discover the barriers that exist in this community when a woman attempts to access prenatal care. A concern for the well being of the children in the community and a belief in the importance of early intervention to enhance outcomes inspired the idea for this project.

Rehabilitation Of Obesity, Patricia Ann Thomas

Theses Digitization Project

This project investigates the many influences throughout the life span that interact to cause obesity. Heredity factors, overfeeding in infancy and childhood, repeated dieting, inactivity, lifestyle and psychosocial conditions all contribute to the incidence of obesity.

Chronic Childhood Disease And Child Abuse, Michelle Marie Lindholm

Theses Digitization Project

The purpose of the present research is to investigate whether or not chronically ill children are victims of child abuse more frequently than healthy children. The gender of the child and of the parent will also be examined for differences in the treatment children receive.

A Constructive View Of The Non-Compliant Patient: Understanding Barriers To Compliance And Proposed Solutions, Barbara Smith

Theses Digitization Project

The health care industry is currently experiencing many changes. Managed care is altering the way health care is delivered through advances in technology and new approaches in financing. As part of this transition, importance will be placed on patients becoming better consumers and participants in determining their health care service needs. The focus of this study is on the non-compliant patient and the many factors that revolve around this topic.

Outlook Magazine, Spring 1998

Outlook Magazine

No abstract provided.