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College Of Osteopathic Medicine Student Handbook July 2002, Nova Southeastern University

Health Professions Divisions Course Catalogs and Course Descriptions

No abstract provided.

Social Networks And Hiv Transmission: The Contextual Dynamics Of Hiv Risk Behaviors, Karen L. Robinson

International Journal of Global Health

Despite advances in public health, biomedical, and social sciences, the Human Immunodeficiency Virus (HIV), which causes Acquired Immune Deficiency Syndrome (AIDS), continues to proliferate. HIV is spread by exposure to infected blood, semen, or vaginal secretions. HIV transmission most commonly occurs because of sexual activities, through the sharing of contaminated needles and other drug paraphernalia, and less frequently, from infected mothers to their newborns (CDC, 2001). From the first reported in case in June 1981 through June 2001, the Center for Disease Control and Prevention (CDC) has reported 793,026 cases of AIDS. The CDC estimates that 650,000 to 900,000 Americans …

Are You My Parent? Are You My Child? The Role Of Genetics And Race In Defining Relationships After Reproductive Technological Mistakes, 5 Depaul J. Health Care L. 15 (2002), Raizel Liebler

UIC Law Open Access Faculty Scholarship

Imagine that you are a married woman who wants to have a genetically related child with your husband. Your doctor tells you that you are infertile, and therefore you and your husband go to XYZ fertility clinic to receive in vitro treatment. You have your eggs harvested, your husband supplies sperm, and ten embryos are created. Five embryos are implanted in your uterus and five are frozen and kept by the fertility clinic for your later use. You successfully conceive and give birth to twins. You notice that the children you give birth to are of a different race than …

Improved Energy Model For Membrane Electroporation In Biological Cells Subjected To Electrical Pulses, R. P. Joshi, Q. Hu, K. H. Schoenbach, H. P. Hjalmarson

Bioelectrics Publications

A self-consistent model analysis of electroporation in biological cells has been carried out based on an improved energy model. The simple energy model used in the literature is somewhat incorrect and unphysical for a variety of reasons. Our model for the pore formation energy E(r) includes a dependence on pore population and density. It also allows for variable surface tension, incorporates the effects of finite conductivity on the electrostatic correction term, and is dynamic in nature. Self-consistent calculations, based on a coupled scheme involving the Smoluchowski equation and the improved energy model, are presented. It is shown that E(r) becomes …

Mechanisms Counteracting Swelling In Brain Cells During Hyponatremia, Herminia Pasantes-Morales, Rodrigo Franco, Benito Ordaz, Lenin D. Ochoa

School of Veterinary and Biomedical Sciences: Faculty Publications

Water gain in the brain consequent to hyponatremia is counteracted by mechanisms that initially include a compensatory displacement of liquid from the interstitial space to cerebrospinal fluid and systemic circulation and subsequently an active reduction in cell water accomplished by extrusion of intracellular osmolytes to reach osmotic equilibrium. Potassium (K⁺), chloride (Cl^–), amino acids, polyalcohols, and methylamines all contribute to volume regulation, with a major contribution of ions at the early phase and of organic osmolytes at the late phase of the regulatory process. Experimental models in vitro show that osmolyte fluxes occur via leak pathways …

The Effect Of Endodontic Solutions On Resorcinol-Formalin Paste, Ronald Nicholas Vranas

Theses and Dissertations

This study determined if any of four endodontic solutions would have a softening effect on resorcinol-formalin paste and if there were any differences in the solvent action between these solutions. Resorcinol, formalin and zinc oxide were mixed and allowed to set for 30 days. The solutions tested were 0.9% sodium chloride, 5.25% NaOCl, chloroform and Endosolv R. Seven samples per solution were tested and an additional seven samples served as controls. Each sample was saturated with one of the solutions and the depth of penetration was tested at 1 min, 2 min, 5 min, 10 min and 20 min using …

Stem Cells, Cloning, And Abortion: Making Careful Distinctions, Dena S. Davis

Law Faculty Articles and Essays

The current controversy over federal funding for research involving stem cells derived from very early embryos is situated between two other equally difficult issues: abortion and cloning. As Laurie Zoloth (2002) says, talk about stem cells is "directly proximate" to the abortion debate. Nonetheless, a settled position in favor of abortion rights does not necessarily lead to support for research that involves the death of embryos. Nor should opposition to reproductive cloning necessarily entail opposition to therapeutic cloning. There are important ways in which our attitudes toward research with embryonic stem cells ought to be entwined with our thinking about …

High-Resolution Physical Map For Chromosome 16q12.1-Q13, The Blau Syndrome Locus, Xiaoju Wang, Helena Kuivaniemi, Gina Bonavita, Charlene J. Williams, Gerard Tromp

Wayne State University Associated BioMed Central Scholarship

Abstract

Background

The Blau syndrome (MIM 186580), an autosomal dominant granulomatous disease, was previously mapped to chromosome 16p12-q21. However, inconsistent physical maps of the region and consequently an unknown order of microsatellite markers, hampered us from further refining the genetic locus for the Blau syndrome. To address this problem, we constructed our own high-resolution physical map for the Blau susceptibility region.

Results

We generated a high-resolution physical map that provides more than 90% coverage of a refined Blau susceptibility region. The map consists of four contigs of sequence tagged site-based bacterial artificial chromosomes with a total of 124 bacterial artificial …

Determining The Level Of Knowledge Of Und College Women In Their Childbearing Years Regarding Exercise During Pregnancy, Tricia Flohr, Sara Hoerner, Kari J. Melby

Physical Therapy Scholarly Projects

With an increase in the number of pregnant women exercising, there is an increased need for patient education and the distribution of updated guidelines to promote appropriate exercise activities.

PURPOSE: The purpose of this study was to determine the level of knowledge in English-speaking, college women in their childbearing years attending the University of North Dakota regarding the risks, precautionary measures and recommended modes of exercise during pregnancy.

SUBJECTS & METHODS: Eighty-four women between the ages of 18 and 40 enrolled in the summer school session completed a thirty-one question survey. The survey was used to gather information about the …

The Herbicide Paraquat Causes Up-Regulation And Aggregation Of Α-Synuclein In Mice: Paraquat And Α-Synuclein, Amy B. Manning-Bog, Alison L. Mccormack, Jie Li, Vladimir N. Uversky, Anthony L. Fink, Donato A. Di Monte

Molecular Medicine Faculty Publications

α-Synuclein-containing aggregates represent a feature of a variety of neurodegenerative disorders, including Parkinson's disease (PD). However, mechanisms that promote intraneuronal α-synuclein assembly remain poorly understood. Because pesticides, particularly the herbicide paraquat, have been suggested to play a role as PD risk factors, the hypothesis that interactions between α-synuclein and these environmental agents may contribute to aggregate formation was tested in this study. Paraquat markedly accelerated the in vitro rate of α-synuclein fibril formation in a dose-dependent fashion. When mice were exposed to the herbicide, brain levels of α-synuclein were significantly increased. This up-regulation followed a consistent pattern, with higher α-synuclein …

Elucidation Of The Molecular Mechanism During The Early Events In Immunoglobulin Light Chain Amyloid Fibrillation: Evidence For An Off-Pathway Oligomer At Acidic Ph, Pierre O. Souillac, Vladimir N. Uversky, Ian S. Millett, Ritu Khurana, Sebastian Doniach, Anthony L. Fink

Molecular Medicine Faculty Publications

Light chain amyloidosis involves the systemic pathologic deposition of monoclonal light chain variable domains of immunoglobulins as insoluble fibrils. The variable domain LEN was obtained from a patient who had no overt amyloidosis; however, LEN forms fibrils in vitro, under mildly destabilizing conditions. The in vitro kinetics of fibrillation were investigated using a wide variety of probes. The rate of fibril formation was highly dependent on the initial protein concentration. In contrast to most amyloid systems, the kinetics became slower with increasing LEN concentrations. At high protein concentrations a significant lag in time was observed between the conformational changes …

How Community Structures Of Time And Opportunity Shape Adolescent Sexual Behavior In South Africa, Carol E. Kaufman, Shelley Clark, Ntsiki Manzini, Julian May

Poverty, Gender, and Youth

Recently, South Africa has led the world in rates of HIV/AIDS infection. Particularly among young adults, the prevalence of HIV has soared, now reaching 25 percent. The statistics suggest a high level of unprotected sex and risky sexual behavior even in the midst of high levels of knowledge. Research has focused on the dynamics of individuals’ and partners’ sexual decisionmaking. Little is known, however, about the larger context in which those decisions are made. What are the everyday experiences that influence risk-taking? Are young people with little to do more likely to engage in risky behavior? Do community opportunities of …

Pregnant Or Positive: Adolescent Childbearing And Hiv Risk In South Africa, Naomi Rutenberg, Carol E. Kaufman, Kate Macintyre, Lisanne Brown, Ali Mehyrar Karim

Poverty, Gender, and Youth

In communities where early age of childbearing is common and HIV prevalence is high, adolescent boys and girls may place themselves at risk of HIV to realize their childbearing preferences. In this paper, we analyze survey data from Kwa-Zulu-Natal province that explores whether an association exists between pregnancy preferences and behavioral and perceptual measures of HIV risk among adolescents in South Africa. Our analysis is based on data from 1,426 sexually active respondents aged 14-22 who participated in wave 1 of the “Transitions to Adulthood in the Context of AIDS in South Africa” study. We use logistic regression to model …

Understanding The Infectious Agent In Nvcjd: Role Of Copper, Edi̇z Demi̇rpençe, Fi̇li̇z Akbiyik

Turkish Journal of Veterinary & Animal Sciences

No abstract provided.

Prevalence Of Asthma And Asthma-Like And Allergic Symptoms In The Urban Adult Population Of Elazığ, Tuncer Tuğ, Yasemi̇n Açik, Esra Tuğ

Turkish Journal of Medical Sciences

The aim of this study was to determine the prevalence of asthma, asthma-like symptoms and atopy in the adult population of the city of Elazığ. A specific questionnaire, adopted from the European Community Respiratory Health Survey including social status and smoking tendencies of people was filled in by 2454 randomly chosen (1088 male, 1366 female) subjects. The cumulative prevalence of asthma and asthma-like symptoms for males, females and the general population was 22.0%, 28.7%, and 25.7%, respectively. The periodic prevalences of them were 17.3%, 20.5%, and 19.1%, while rates of previously diagnosed asthma were 2.6%, 3.5%, and 3.1%. Subjects suffering …

Digest Of The Philadelphia College Of Osteopathic Medicine (Winter 2002), Philadelphia College Of Osteopathic Medicine

Digest

The Winter 2002 issue of the Digest of the Philadelphia College of Osteopathic Medicine includes the following articles:

• Opening Remarks
• PCOM Updates
• Founders’ Day 2002: Presidential Inauguration
• College Bestows Highest Award on Joseph M. Pellettiere, Jr., DO ’48
• Mason W. Pressly Memorial Medal Awarded
• The Inflammation Puzzle
• Kenya Trip Opens Student’s Eyes
• Class Notes
• In Memoriam
• My Turn
• Alumni Weekend 2002
• PCOM Events

Dynamics Of Bovine Tuberculosis In Wild White-Tailed Deer In Michigan, Graham J. Hickling

Michigan Bovine Tuberculosis Bibliography and Database

In mid-2001, the author of this report was invited to undertake an exploratory analysis of Michigan Department of Natural Resources’ data on bovine tuberculosis (TB) infection in wild white-tailed (Odocoileus virginianus) in the northeastern Lower Peninsula of the state. The aims of this analysis were:
• to quantify the geographic spread of tuberculous deer in Michigan;
• to investigate key factors influencing the prevalence of disease in the core of the infected area;
• to interpret the results in light of overseas experience with wildlife TB, with a view to
recommending how current management of the disease might …

Tonsillar Lesions In White-Tailed Deer (Odocoileus Virginianus) Naturally Infected With Mycobacterium Bovis, M. V. Palmer, D. L. Whipple, K. L. Butler, S. D. Fitzgerald, C. S. Bruning-Fann, S. M. Schmitt

Michigan Bovine Tuberculosis Bibliography and Database

In 1994, a free-living white-tailed deer (Odocoileus virginianus) in Michigan was diagnosed with tuberculosis caused by Mycobacterium bovis (Schmitt and others 1997). Subsequent surveys conducted by the Michigan Department of Natural Resources and Michigan State University Animal Health Diagnostic Laboratory identified an epidemic of M bovis infection in free-living white-tailed deer in northeast Michigan (Schmitt and others 1997, O'Brien and others 2001). This represents the first known reservoir of M bovis in free-living wildlife in the USA, and the first known epidemic of tuberculosis in white-tailed deer anywhere in the world.

Mycobacterium Bovis–Infected White-Tailed Deer (Odocoileus Virginianus): Detection Of Immunoglobulin Specific To Crude Mycobacterial Antigens By Elisa, W. Ray Waters, Mitchell V. Palmer, Diana L. Whipple

Michigan Bovine Tuberculosis Bibliography and Database

White-tailed deer (Odocoileus virginianus) have recently emerged as a source of Mycobacterium bovis infection for cattle within North America. The objective of this study was to evaluate the antibody response of M. bovis–infected deer to crude mycobacterial antigens. Deer were experimentally inoculated with M. bovis strain 1315 either by intratonsilar instillation or by exposure to M. bovis–infected (i.e., in contact) deer. To determine the time course of the response, including the effects of antigen administration for comparative cervical skin testing, serum was collected periodically and evaluated by enzyme-linked immunosorbent assay (ELISA) for immunoglobulin (i.e., IgG heavy …

Nitric Oxide Production As An Indication Of Mycobacterium Bovis Infection In White-Tailed Deer (Odocoileus Virginianus), W. R. Waters, M. V. Palmer, R. E. Sacco, D. L. Whipple

Michigan Bovine Tuberculosis Bibliography and Database

White-tailed deer ( Odocoileus virginianus ) are reservoirs for Mycobacterium bovis in northeast Michigan, USA. Production of nitric oxide (NO) by activated macrophages is a potent mechanism of mycobacterial killing. The capacity of macrophages to produce NO, however, varies among mammalian species. The objective of this study was to determine if mononuclear cells from white-tailed deer produce nitrite as an indication of NO production and, if so, is NO produced in response to stimulation with M. bovis antigens. Supernatants were harvested from adherent peripheral blood mononuclear cell (PBMC) cultures that had been stimulated with either Mannheimia haemolytica lipopolysaccharide (LPS) or …

Bovine Tuberculosis In Michigan Wildlife And Livestock, Stephen M. Schmitt, Daniel J. O'Brien, Colleen S. Brunning-Fann, Scott D. Fitzgerald

Michigan Bovine Tuberculosis Bibliography and Database

Since 1994, the state of Michigan has recognized a problem with bovine tuberculosis (TB), caused by Mycobacterium bovis, in wild white-tailed deer from a 12-county area in northeastern Lower Michigan. A total of 65,000 free-ranging deer have been tested, and 340 have been found to be positive for M. bovis. The disease has been found in other wildlife species, and, in 1998, in domestic cattle, where to date 13 beef cattle and 2 dairy cattle herds have been diagnosed with bovine TB. Unfortunately, the situation is unique in that there have never been reports of self-sustaining bovine TB …

Mycobacterium Smegmatis D-Alanine Racemase Mutants Are Not Dependent On D-Alanine For Growth, Ofelia Chacon, Zhengyu Feng, N. Beth Harris , Nancy E. Caceres, Garry Adams, Raul G. Barletta

School of Veterinary and Biomedical Sciences: Faculty Publications

Mycobacterium smegmatis is a fast-growing nonpathogenic species particularly useful in studying basic cellular processes of relevance to pathogenic mycobacteria. This study focused on the D-alanine racemase gene (alrA), which is involved in the synthesis of D-alanine, a basic component of peptidoglycan that forms the backbone of the cell wall. M. smegmatis alrA null mutants were generated by homologous recombination using a kanamycin resistance marker for insertional inactivation. Mutants were selected on Middlebrook medium supplemented with 50 mM D-alanine and 20 μg of kanamycin per ml. These mutants were also able to grow in standard and minimal media without …

Derivation Of Risk Based Wipe Surface Screening Levels For Industrial Scenarios, Lisa M. May, Bonnie Gaborek, Tony Pitrat, Laura Peters

Uniformed Services University of the Health Sciences

The environmental characterization of building interiors and other surfaces has generally been performed with wipe-sampling because it is a non-destructive technique. There is no consensus, however, as to the interpretation of the results of wipe-sampling. Specifically, there is not a standardized method to determine if chemicals found at sampled levels pose a threat to human health. A methodology was developed, based on acceptable health risk levels, to derive screening levels for evaluating wipe-sampling results pertaining to industrial scenarios. The methodology was based on the United States Environmental Protection Agency (USEPA) Region IX Preliminary Remediation Goal (PRG) approach; a multi-exposure methodology …

Mutations In The Yeast Lcb1 And Lcb2 Genes, Including Those Corresponding To The Hereditary Sensory Neuropathy Type I Mutations, Dominantly Inactivate Serine Palmitoyltransferase, Ken Gable, Gongshe Han, Erin Monaghan, Dagmar Bacikova, Mukil Natarajan, Robert W. Williams, Teresa M. Dunn

Uniformed Services University of the Health Sciences

It was recently demonstrated that mutations in the human SPTLC1 gene, encoding the Lcb1p subunit of serine palmitoyltransferase (SPT), cause hereditary sensory neuropathy type I (1, 2). As a member of the subfamily of pyridoxal 5'-phosphate enzymes known as the α-oxoamine synthases, serine palmitoyltransferase catalyzes the committed step of sphingolipid synthesis. The residues that are mutated to cause hereditary sensory neuropathy type I reside in a highly conserved region of Lcb1p that is predicted to be a catalytic domain of Lcb1p on the basis of alignments with other members of the α-oxoamine synthase family. We found that the corresponding mutations …

Increased Production Of Pro-Inflammatory Cytokines And Enhanced T Cell Responses After Activation Of Human Dendritic Cells With Il-1 And Cd40 Ligand, Amy Wesa, Anne Galy

Wayne State University Associated BioMed Central Scholarship

Abstract

Background

Various microbial, inflammatory and immune signals regulate the activation of dendritic cells (DC), determining their ability to interact with naïve T cells and to produce cytokines that direct T cell development. In particular, CD40L and IL-1 cooperatively activate DC to secrete high levels of IL-12. The immuno-stimulatory capacity of such DC is otherwise not well-defined prompting further characterization of the effects of IL-1 and family members on DC activation in comparison with other pro-inflammatory stimuli.

Results

Human DC co-activated in vitro by CD40L and IL-1β expressed numerous cytokine genes including IL-12β, IL-23 p19, IL-1β, IL-1α, IL-1Ra, IL-10, IL-6, …

Search For Intracranial Aneurysm Susceptibility Gene(S) Using Finnish Families, Jane M. Olson, Sompong Vongpunsawad, Helena Kuivaniemi, Antti Ronkainen, Juha Hernesniemi, Markku Ryynã¤Nen, Lee-Lian Kim, Gerard Tromp

Wayne State University Associated BioMed Central Scholarship

Abstract

Background

Cerebrovascular disease is the third leading cause of death in the United States, and about one-fourth of cerebrovascular deaths are attributed to ruptured intracranial aneurysms (IA). Epidemiological evidence suggests that IAs cluster in families, and are therefore probably genetic. Identification of individuals at risk for developing IAs by genetic tests will allow concentration of diagnostic imaging on high-risk individuals. We used model-free linkage analysis based on allele sharing with a two-stage design for a genome-wide scan to identify chromosomal regions that may harbor IA loci.

Methods

We previously estimated sibling relative risk in the Finnish population at between …

Comparison Of Antibiotic Resistance Patterns Of Uropathogenic Escherichia Coli In Outpatient And Inpatient Populations From 1990 To 1996 In Bemidji, Mn, Gail R. Conway, Patrick Guilfoile

Journal of the Minnesota Academy of Science

A retrospective study of antibiotic susceptibility was conducted on data from all uropathogenic Escherichia coli submitted to the MeritCare Clinic-Bemidji, MN and the North Country Regional Hospital-Bemidji, MN during 1990, 1993, and 1996. The proportion of antibiotic resistant strains was generally equal or higher in E. coli isolates from community-acquired infections than with E. coli isolates from nosocomial infections. Comparison with antibiotic prescriptions during 1990, 1993, and 1996 provided no clear relation between antibiotics prescribed for outpatient urinary tract infections and the proportion of E. coli strains resistant to those antibiotics. This suggests that factors other than selection for resistance …

Synapsis: Philadelphia Campus (2002), Philadelphia College Of Osteopathic Medicine

All Yearbooks

This yearbook includes Trips, Formal, Halloween, Parties, Clinics, AIDS Walk, Clubs, A Capella, American Holistic Medical Association, AHMA, American College Of Osteopathic Family Practitioners, ACOFP, Association Of Military Osteopathic Physicians and Surgeons, AMOPS, American Medical Association Medical Student Society, AMA-MSS, American Medical Women's Association, AMWA, Asian Pacific American Medical Student Association, APAMSA, American Medical Student Association, AMSA, Christian Medical And Dental Society, CMDS, Emanuel Fliegelman Obstetrics and Gynecology, Ob/Gyn Club, Daniel Wisely Surgery, Emergency Medicine, Founders' Day Follies, Follies, Geriatrics, Internal Medicine, Phi Sigma Gamma, PSG, (ΦΣΓ), National Boricua Latino Health Organization, NBLHO, Physicians for Social Responsibility. PSR, Public Health, …

Serotonin Transporter Promoter Polymorphism, Peripheral Indexes Of Serotonin Function, And Personality Measures In Families With Alcoholism, Scott F. Stoltenberg, Geoffrey R. Twitchell, Gregory L. Hanna, Edwin H. Cook, Hiram E. Fitzgerald, Robert A. Zucker, Karley Y. Little

Department of Psychology: Faculty Publications

A functional polymorphism in the regulatory region of the serotonin transporter gene (5-HTTLPR) is considered to be a plausible candidate gene for anxiety-related personality traits and for alcoholism. Empirical support for the association between 5-HTTLPR and psychological traits has been somewhat inconsistent; however, observations of the functional dominance of the low-activity s-allele over the l-allele have been more consistent. When studying the influence of particular genes on psychological traits, it seems useful also to assess more biological intermediate traits that may mediate the effects of those genes on the traits of interest. The present study examined relationships between 5-HTTLPR genotype, …

Antisocial Alcoholism And Serotonin-Related Polymorphisms: Association Tests, Elizabeth M. Hill, Scott F. Stoltenberg, Katherine Harris Bullard, Sheng Li, Robert A. Zucker, Marget Burmeister

Department of Psychology: Faculty Publications

Central serotonin dysfunction appears to be related to a subtype of alcoholism with antisocial impulsive features (type II; antisocial alcoholism). The serotonergic deficit may be associated with greater impulsivity, which in turn facilitates both alcohol dependence and antisocial behavior. The present study tested association of antisocial impulsive alcoholism with candidate genes related to serotonergic neurotransmission, using families. Eight markers were assayed using polymerase chain reaction: tryptophan hydroxylase (intron 7), the serotonin transporter SLC6A4 (VNTR 9/12), HTTLPR, the three serotonin receptor types HTR1B (G861C), HTR2A (T102C) and HTR2C (Cys23Ser), monoamine oxidase A (T1460C), and (CA)_n. Eligible probands had early …