Digital Commons Network^™

Virginia Dental Journal (Vol. 79, No. 4, 2002)

Virginia Dental Journal

No abstract provided.

Comparison Of Hydrostatic Weighing And Plethysmography Techniques For The Development Of Skinfold Prediction Equations For Children, Tomoko Tozuka

Theses, Dissertations and Capstones

Regression equations between skinfold (SKF) measurements and air displacement plethysmography (ADP) were determined 6 to 18 year old athletes (80 males / 34 females) using Pearson r correlation coefficients. Triceps and mid-calf SKF and percent body fat (%BF) by ADP were measured during an Athletic Ability Assessment at The HIT Center (Huntington, WV). A high relationship was found between SKF and %BF by ADP (males; r² = .76, females; r² = .74, all; r² = .77). Data from the subjects were compared with similar correlations determined between SKF and %BF by hydrostatic weighing (HW) from another study …

Department Of Veterinary And Biomedical Sciences: 2002 Annual Report

School of Veterinary and Biomedical Sciences: Information and History

Foreword 2002
VBMS Faculty
VBMS Faculty and Staff, by Function and Unit
Honors. Awards and Recognitions. 2002
VBMS Committee Assignments. 2002
Faculty Profiles
VBMS Research Associates Profiles
VBMS Teaching Program
Great Plains Veterinary Educational Center (GPVEC)
VBMS Research Program
International Activities
Veterinary Extension Program
Nebraska Veterinary Diagnostic Laboratory Systems (NVDLS)
VBMS Grants and Contracts Program
Patents by VBMS Faculty in 2002
Publications by VBMS Faculty
Presentations by VBMS Faculty
Articles Regarding the Department
Selected Committees. Editorial and Other Appointments
Departmental Budget Summaries
Nebraska Agricultural Statistics

Pathogenesis Of Human And Bovine Cryptosporidium Parvum In Gnotobiotic Pigs, Sonia J. Pereira, Norma E. Ramirez, Lihua Xiao, Lucy A. Ward

United States Public Health Resources

To compare the pathogenesis of human genotype 1 (HuGl) and bovine genotype 2 (BoG2) Cryptosporidium parvum, neonatal gnotobiotic pigs were given 1-10 HuGl or BoG2 oocysts. The prepatent and patent periods were significantly longer for HuGl than for BoG2 C. parvum (prepatent, 8.6 vs. 5.6 days; patent, 16.6 vs. 10.3 days). BoG2-infected pigs developed signif- icantly more severe disease than did HuGl-infected pigs. BoG2 parasites were seen micro- scopically throughout the intestines during the prepatent and patent periods. HuGl parasites were only detected during the patent period in the ileum and colon but colonized the mucosal surface in significantly …

A Preliminary Linkage Map Of The Tick, Ixodes Scapularis, Amy J. Ullmann, Joseph Piesman, M.C. Dolan, William C. Black, Iv

United States Public Health Resources

A linkage map of the Ixodes scapularis genome was constructed based upon segregation amongst 127 loci. These included 84 random amplified polymorphic DNA (RAPD) markers, 32 Sequence-Tagged RAPD (STAR) markers, 5 cDNAs, and 5 microsatellites in 232 F₁ intercross progeny from a single, field-collected P₁ female. A preliminary linkage map of 616 cM was generated across 14 linkage groups with one marker every 10.8 cM. Assuming a genome size of ∼10⁹ bp, the relationship of physical to genetic distance is ∼300 kb/cM in the I. scapularis genome.

Anopheles Gambiae Genome: Completing The Malaria Triad, Kathryn S. Aultman, Michael Gottlieb, Maria Y. Giovanni, Anthony S. Fauci

United States Public Health Resources

The genome sequence of Anopheles gambiae, the major mosquito vector of the malaria parasite Plasmodium falciparum, as well as analyses of its gene content and organization, are reported by several teams of investigators in this issue. In addition, the sequence of the P. falciparum genome appears in this week’s issue of Nature. The genomes of these two organisms, along with that of the human, provide a triad of critical genetic information relevant to all stages of the malaria transmission cycle and offer unprecedented opportunities to the scientific and public health communities engaged in the fight against malaria, …

Real-Time Fluorescence Pcr Assays For Detection And Characterization Of Shiga Toxin, Intimin, And Enterohemolysin Genes From Shiga Toxin-Producing Escherichia Coli, Udo Reischl, Mohammad T. Youssef, Jochen Kilwinski, Norbert Lehn, Wen Lan Zhang, Helge Karch, Nancy Strockbine

United States Public Health Resources

PCR assays have proved useful for detecting and characterizing Shiga toxin-producing Escherichia coli (STEC). Recent advances in PCR technology have facilitated the development of real-time fluorescence PCR assays with greatly reduced amplification times and improved methods for the detection of amplified target sequences. We developed and evaluated two such assays for the LightCycler instrument: one that simultaneously detects the genes for Shiga toxins 1 and 2 (stx₁ and stx₂) and another that simultaneously detects the genes for intimin (eae) and enterohemolysin (E-hly). Amplification and sequence-specific detection of the two target genes were …

Increased Hippocampal Volume In Schizophrenics' Parents With Ancestral History Of Schizophrenia, Josette G. Harris, David A. Young, Donald C. Rojas, Ana Cajade-Law, Ann Scherzinger, Safia Nawroz, Lawrence E. Adler, C. Munro Cullum, Jack Simon, Robert Freedman

United States Public Health Resources

Objective: Decreased hippocampal volume is one of the hypothesized pathological features of schizophrenia, but it is not known if this abnormality is familially transmitted. The aim of this study was to measure the hippocampal volume of the parents of schizophrenic probands. in relationship to the apparent transmission of genetic risk.
Method: Eighteen subjects from families consisting of a schizophrenic proband and two clinically unaffected parents were studied. Probands were compared to six control subjects, matched for age, sex, and educational level. The six families were selected so that only one parent had an ancestral family history of schizophrenia. The volumes …

Inhibition Of Hepatic Stellate Cell Collagen Synthesis By N-(Methy1amino)Isobutyric Acid, Thomas L. Freeman, Kusum K. Kharbanda, Dean Tuma, Mark Milliard

United States Public Health Resources

The increased deposition of extracellular matrix by hepatic stellate cells following liver injury, in a process known as activation, is considered a key mechanism for increased collagen content of liver during the development of liver fibrosis. We report that N- (methylamino)isobutyric acid (MeAIB), a specific inhibitor of System A-mediated amino acid uptake, reduces the accumulation of collagen in CFSC-2G hepatic stellate cell cultures and in a rat model of liver injury and fibrosis. Rat CFSC-2G cells were cultured in 0-5 mM MeAIB, and the accumulation and synthesis of collagen were measured by binding to Sirius red F3B and pulse-labeling with …

Effect Of Malondialdehyde-Acetaldehyde-Protein Adducts On The Protein Kinase C-Dependent Secretion Of Urokinase-Type Plasminogen Activator In Hepatic Stellate Cells, Kusum K. Kharbanda, Kris A. Shubert, Todd A. Wyatt, Michael F. Sorrell, Dean J. Tuma

United States Public Health Resources

Previous studies from our laboratory have shown that malondialdehyde-acetaldehyde-protein adducts (MAA adducts) are formed in hepatocytes of ethanol-fed rats and directly influence the hepatic stellate cells (HSCs) to induce their secretion of chemokines and to up-regulate their expression of adhesion molecules. Since protein kinase C (PKC) is known to play a major role in many diverse intracellular signal transduction processes, we investigated whether MAA adducts influence the function of HSCs via a PKC-dependent pathway. HSCs in culture were exposed to MAA adducts, and PKC activity was determined. We observed a time- and concentration-dependent activation of PKC when cultures were exposed …

Ata2-Mediated Amino Acid Uptake Following Partial Hepatectomy Is Regulated By Redistribution To The Plasma Membrane, Thomas L. Freeman, Geoffrey M. Thiele, Dean J. Tuma, Tina K. Machu, Mark E. Mailliard

United States Public Health Resources

System A, the Na⁺-dependent amino acid transport activity, is encoded by the ATA2 gene and up-regulated following partial hepatectomy (PH), and its competitive inhibition interferes with liver regeneration. Rabbit polyclonal antibody was raised against a portion of the ATA2 gene product followed by immunodetection of ATA2 in isolated liver plasma membrane and lysate. The level of ATA2 increased in the plasma membrane following PH, while the relatively high quantity of ATA2 found in liver lysate remained constant. We also have shown that Northern analysis of steady-state ATA2 mRNA revealed no significant change following PH. These data show that …

Susceptibility Of Raccoons (Procyon Lotor) To Infection With Mycobacterium Bovis, Mitchell V. Palmer, W. Ray Waters, Diana L. Whipple

Michigan Bovine Tuberculosis Bibliography and Database

Tuberculosis due to Mycobacterium bovis infection is endemic in white-tailed deer (Odocoileus virginianus) in the northeastern portion of the lower Michigan peninsula (USA). Various wild carnivores and omnivores, including raccoons (Procyon lotor), are infected with M. bovis within the endemic area. To investigate the pathogenesis of tuberculosis in raccoons and the likelihood of M. bovis transmission from infected raccoons to other susceptible hosts, we experimentally inoculated raccoons with single oral doses of M. bovis (ranging from 30 to 1.7 x 10⁵ colony forming units [CFU]), five daily oral doses of M. bovis (ranging from 10 …

Co-Occurring Disorders Problem Gambling Integrated Treatment Workbook, Kathleen Moore, Chad O. Matthews, W. Michael Hunt, Laura Pape

Mental Health Law & Policy Faculty Publications

In this stage (which will be discussed in Module 8 in more detail) the client has achieved abstinence for at least six months. As relapse of substance use and gambling disorders is common, one important goal is to help maintain an awareness that relapse is possible and to take steps to minimize the chan ces of relapse occurring. A second goal of this stage is to expand the client’s recovery to other areas of functioning such as social relationships and health. Comm on strategies include developi ng a relapse prevention plan, participating in self-help groups , and working on rehabilitation.

Co-Occurring Disorders Treatment Manual, Kathleen A. Moore, Chad Matthews, W. Michael Hunt, Laura Pape

Mental Health Law & Policy Faculty Publications

This manual was created over a period of several months through the efforts of a working group comprised of substance abuse treatment practitioners and researchers affiliated with the Tampa PIC/Suncoast Practice and Research Collaborative project. The manual was field tested in several treatment agencies in the Tampa Bay area, and was then refined through feedback received by practitioners and clients regarding the manual’s utility, ease of comprehension, and perceived relevance of the material to their needs.

This manual provides a guide for conducting treatment groups related to co-occurring substance use and mental health disorders. Counselors should feel free to adapt …

(Women And) Children First: Applicable To Lifeboats? Applicable To Human Experimentation?, Lainie Friedman Ross, M. Justin Coffey

Journal of Health Care Law and Policy

No abstract provided.

Determination Of Alpha-I Antitrypsin Genetic Deficiency In Duodenal Ulcer By Polymerase Chain Reaction, A Shahid, H Qureshi, S J. Zuberi, Anwar Ali Siddiqui, T Sultana, M A. Waqar

Department of Biological & Biomedical Sciences

Objective: To confirm alpha-I-AT deficiency status in duodenal ulcer using a combination of PCR and restricted enzyme digestion.

Methods: Fifty patients with endoscopically proven duodenal ulcer and hundred controls with no signs of the disease were included. Alpha-i-AT phenotypes were confirmed by polymerase chain reaction followed by restriction enzyme digestion.

Results:Alpha-I-AT concentration in duodenal ulcer patients showed a mean value of 2.12 ± 0.11g/1 (range: 0.52-3.95 g/1, p

Conclusion: Alpha-1 AT deficiency was found in 10% of duodenal ulcer patients. DNA analysis more accurately resolved the phenotypes as S and Z mutations (JPMA 52:545; 2002).

Bioterrorism, Public Health, And International Law, David P. Fidler

Articles by Maurer Faculty

No abstract provided.

Hiv/Aids Prevention Guidance For Reproductive Health Professionals In Developing-Country Settings, Helen Epstein, Daniel Whelan, Janneke Van De Wijgert, Purnima Mane, Suman Mehta

HIV and AIDS

While the integration of information, technologies, and services to respond to HIV/AIDS may seem closely related, there are a variety of issues that continue to create obstacles to integration. Among these are the stigmatizing nature of HIV infection and AIDS and discrimination faced by those who are infected or perceived to be infected; sexual practices and identities that remain socially unacceptable; gender roles and relations that make it difficult for women and men to access information, services, and technologies on HIV prevention; reluctance to recognize the special needs of young people; and barriers to service delivery created by broader economic, …

Allocating Health Care Resources To The Elderly, George P. Smith Ii

Scholarly Articles

Health is not an absolute condition, but is assessed by reference to age and other factors. Therefore, a relative scale is necessary to determine whether an elderly person’s right to health care is being satisfied. Today, older Americans are increasingly affected by the growing need to regulate health care delivery. Since 1900, those over the age of 85 years have become twenty-one times as numerous in society, with an eightfold increase in the number of people over the age of 65. Given the need to curb rising health care costs — particularly the expenses related to caring for older individuals …

Regulation Of Research With Children: The Evolution From Exclusion To Inclusion, Duane Alexander

Journal of Health Care Law and Policy

No abstract provided.

Pediatric Priorities: Legislative And Regulatory Initiatives To Expand Research On The Use Of Medicines In Pediatric Patients, Michael S. Labson

Journal of Health Care Law and Policy

No abstract provided.

Activation Of Central Melanocortin Pathways By Fenfluramlne, Lora K. Heisler, Michael A. Cowley, Laurence H. Tecott, Wei Fan, Malcolm J. Low, James L. Smart, Marcelo Rubinstein, Jeffrey B. Tatro, Jacob N. Marcus, Henne Holstege, Charlotte E. Lee, Roger D. Cone, Joel K. Elmquist

Faculty Publications - Department of Biological & Molecular Science

D-fenfluramine (d-FEN) was once widely prescribed and was among the most effective weight loss drugs, but was withdrawn from clinical use because of reports of cardiac complications in a subset of patients. Discerning the neurobiology underlying the anorexic action of d-FEN may facilitate the development of new drugs to prevent and treat obesity. Through a combination of functional neuroanatomy, feeding, and electrophysiology studies in rodents, we show that d-FEN-induced anorexia requires activation of central nervous system melanocortin pathways. These results provide a mechanistic explanation of d-FEN's anorexic actions and indicate that drugs targeting these downstream melanocortin pathways may prove to …

Medical, Social And Legal Implications Of Treating Nausea And Vomiting Of Pregnancy, Robert L. Brent

The Selected Works of Robert Brent

This article will deal with medical, social, and legal implications of treating nausea and vomiting of pregnancy (NVP). Clinical problems occur when the symptoms become exaggerated and result in debilitation, dehydration, and hospitalization. The treatment of NVP in its early stages has the implication that it will prevent the more serious complications, including hospitalization. Therapeutic modalities discussed in this conference that have been used or are being tested are primarily symptomatic treatments (antihistamines, Bendectin (Merrell Dow; Cincinatti, Ohio), phenothiazines, hyponosis, accupressure, relaxation behavioral modification, audiogenic feedback training, newer medications, diet, and nutritional support). Bendectin is probably the most studied medication …

The Effects Of Magnetic Insoles On Lumbar Flexion, Kari Braaflat, Becky Cox, Angela Delacruz, Heather Skarsgard

Physical Therapy Scholarly Projects

Magnets have been used for therapeutic purposes for hundreds of years. Today, magnetic devices and other alternative therapies are more popular than ever among the general public. The effect of these magnetic devices is dependent on several variables including strength, depth of penetration, placement, time exposed to the magnetic field, and polarity. There are many proposed theories on how magnets affect the body, but there is little research to support them. One of the theories proposed is that magnets alter connective tissue elasticity and allow greater range of motion (ROM). Additional research needs to be conducted to validate or negate …

Reliability Of Shoulder Goniometric Measurements In Persons Who Are Elderly, Karina Koch, Lauri Paulsen, Heather White, Richard Williamson

Physical Therapy Scholarly Projects

The population of the United States is rapidly aging; this is exemplified by the cohort of "Baby-Boomers" who make up a large part of the population. This becomes relevant to physical therapists, as our clients will reflect this "graying" of America. There is a lack of research regarding normative values for shoulder range of motion (ROM) in the elderly population. Due to many different factors in the aging process, there may be a decline in ROM in elderly persons. Current normative values may not be applicable to this population.

Two pilot studies were conducted measuring active and passive shoulder ROM …

Biophysical Properties Of The Synucleins And Their Propensities To Fibrillate: Inhibition Of Α-Synuclein Assembly By Β- And Γ-Synucleins, Vladimir N. Uversky, Jie Li, Pierre O. Souillac, Ian S. Millett, Sebastian Doniach, Ross Jakes, Michael Goedert, Anthony L. Fink

Molecular Medicine Faculty Publications

The pathological hallmark of Parkinson's disease is the presence of intracellular inclusions, Lewy bodies, and Lewy neurites, in the dopaminergic neurons of the substantia nigra and several other brain regions. Filamentous α-synuclein is the major component of these deposits and its aggregation is believed to play an important role in Parkinson's disease and several other neurodegenerative diseases. Two homologous proteins, β- and γ-synucleins, are also abundant in the brain. The synucleins are natively unfolded proteins. β-Synuclein, which lacks 11 central hydrophobic residues compared with its homologs, exhibited the properties of a random coil, whereas α- and γ-synucleins were slightly more …

The Hiv Protease Inhibitor Saquinavir Impairs Lipid Metabolism And Glucose Transport In Cultured Adipocytes, S. Ranganathan, Philip A. Kern

Clinical and Translational Science Faculty Publications

Treatment of HIV infection using protease inhibitors is frequently associated with lipodystrophy and impaired lipid and glucose metabolism. We examined the effect of saquinavir, one of the protease inhibitors, on lipid metabolism and glucose transport in cultured adipocytes. Saquinavir inhibited lipoprotein lipase (LPL) activity in 3T3-F442A and 3T3-L1 adipocytes. The inhibition of LPL was 81% at a concentration of 20 μg/ml. Another closely related drug, indinavir, had a small inhibitory effect. Saquinavir also inhibited the biosynthesis of lipids from [¹⁴C]-acetate. Saquinavir increased the lipolysis. Saquinavir had no significant effect on the cellular protein synthesis or protein content. Saquinavir …

Occupational Therapy Program 2002, Nova Southeastern University

Health Professions Divisions Course Catalogs and Course Descriptions

No abstract provided.

College Of Osteopathic Medicine Student Handbook July 2002, Nova Southeastern University

Health Professions Divisions Course Catalogs and Course Descriptions

No abstract provided.

Social Networks And Hiv Transmission: The Contextual Dynamics Of Hiv Risk Behaviors, Karen L. Robinson

International Journal of Global Health

Despite advances in public health, biomedical, and social sciences, the Human Immunodeficiency Virus (HIV), which causes Acquired Immune Deficiency Syndrome (AIDS), continues to proliferate. HIV is spread by exposure to infected blood, semen, or vaginal secretions. HIV transmission most commonly occurs because of sexual activities, through the sharing of contaminated needles and other drug paraphernalia, and less frequently, from infected mothers to their newborns (CDC, 2001). From the first reported in case in June 1981 through June 2001, the Center for Disease Control and Prevention (CDC) has reported 793,026 cases of AIDS. The CDC estimates that 650,000 to 900,000 Americans …